CN103435714A - Preparation and purification methods and application of fig crude polysaccharide - Google Patents
Preparation and purification methods and application of fig crude polysaccharide Download PDFInfo
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Abstract
The invention discloses a preparation method of fig crude polysaccharide, which comprises the following steps: taking dried fig, pulverizing, refluxing with 95% ethanol to remove grease and pigments, carrying out vacuum filtration, and refluxing the residues with 70% ethanol to remove small molecule impurities; taking the residues as the raw material for extracting fig polysaccharide; and taking the residues, extracting under microwave conditions by using water as a solvent, carrying out vacuum filtration, concentrating the filtrate to 100Ml by rotary evaporation, adding 95% ethanol, standing for 24 hours, centrifugating, taking the precipitate, removing proteins from the precipitate by a Sevage process, and carrying out freeze-drying to obtain fig crude polysaccharide. In the extraction process, the ratio of the residues to the water is 1:(20-23), the microwave power is 550W, and the extraction time is 13 minutes; and the ethanol for alcohol precipitation is 3.50-3.60 times by volume of the extracting solution. The invention also discloses a purification method and application of the fig crude polysaccharide.
Description
Technical field
The present invention relates to a kind of preparation method of polysaccharide, particularly a kind of preparation method of Fructus Fici Crude polysaccharides; The invention still further relates to the purification process of the Fructus Fici Crude polysaccharides that preceding method makes; The invention still further relates to Fructus Fici Crude polysaccharides or the purposes of fig polysaccharide sterling in preparing alpha-glucosidase inhibitor.
Background technology
Fructus Fici Ficus carica. L is subordinate to the Moraceae Ficus, and edible is partly its dry holder, not only has a sweet taste, and also has nutritive value and pharmaceutical use widely.It is reported, Fructus Fici can be used for strengthening the stomach and promoting bowel movement, subduing swelling and detoxicating, and can suppress tumour, raising immunity of organisms.Fructus Fici is rich in polysaccharide, and polysaccharide has multiple physiologically active, as antitumor, immunomodulatory, antibiotic, anti-oxidant, Inhibiting α-glucosidase etc.All can from document, find experimental basis about the biological activity such as antitumor, but it suppresses effect nobody research of glucuroide, alpha-glucosidase energy catalysis α-1, the 4-hydrolysis of glycoside bond, it is the enzyme of the oligosaccharides hydrolysis such as maltose in small intestine, sucrose, the activity of Inhibiting α-glucosidase, can make the generation of glucose and absorption slow down, and reduces Postprandial peak glucose.Therefore the alpha-glucosidase inhibitor novel type-II diabetes medication that is a class, can be used for postprandial blood sugar and regulate, and keeps patient's postprandial blood sugar steady.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, and the preparation method of a kind of method advantages of simple, the Fructus Fici Crude polysaccharides that easy to operate, yield is high is provided.
Another technical problem to be solved by this invention has been to provide the purification process of Fructus Fici Crude polysaccharides prepared by aforesaid method.
The fig polysaccharide sterling that the Fructus Fici Crude polysaccharides that the method for the invention makes or described purification process purifying obtain can be for the preparation of alpha-glucosidase inhibitor.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is a kind of preparation method of Fructus Fici Crude polysaccharides, is characterized in, its step is as follows: get that pulled figs is some to be shredded, with 95% alcohol reflux degrease and pigment, after suction filtration, residue removes small molecular weight impurity with 70% alcohol reflux; Then get residue, as the extraction raw material of fig polysaccharide; Get residue, after water extracts under microwave condition as solvent, suction filtration, get filtrate and be concentrated into 100Ml with rotary evaporation, adds standing 24h after 95% ethanol, gets precipitation after centrifugal, and precipitation is by the Sevage method except after Deproteinization, and lyophilize, obtain the Fructus Fici Crude polysaccharides; During extraction, the solid-liquid ratio 1:20-23 of residue and water; Microwave power is 550W, and extraction time is 13min, and the 3.50-3.60 that alcohol precipitation is extracting liquid volume by amount of alcohol doubly.
In the preparation method of Fructus Fici Crude polysaccharides of the present invention, most preferred technical characterictic is: during extraction, and the solid-liquid ratio 1:22.45 of residue and water; Alcohol precipitation is extracting liquid volume by amount of alcohol 3.55 times.
The invention also discloses a kind of purification process of the Fructus Fici Crude polysaccharides made as method as described in above technical scheme, be characterized in, its step is as follows:
(1) ion-exchange cellulose DEAE-52 is after the abundant swelling of distilled water, use again the distilled water repetitive scrubbing with the NaOH of 0.5 M and the HCl alternate treatment of 0.5 M for several times, until its pH to 6 is filled post, adopt 2.0cm * 30 cm normal pressure core glass columns, it is stand-by that the ion column installed carries out balance 24 hours with distilled water again;
(2) getting the Fructus Fici Crude polysaccharides take distilled water and is mixed with the solution that mass concentration is 1%; Then by DEAE-52 ion exchange column that on fig polysaccharide solution, balance is good, the loading volume is 5 mL, cross post with 0-1M NaCl 200 mL with the flow velocity of 50 mL/h, with automatic Fraction Collector, with the amount of every pipe 5mL, collected, total sugar concentration with the every pipe of phenolsulfuric acid colorimetric method for determining is made elution curve, collect identical component according to elution curve, in 40 ℃ of concentrating under reduced pressure lyophilize, obtain fig polysaccharide;
(3) Sephacryl S-300HR softly washs for several times, reduces pressure after degassed through distilled water and fills post, adopts 1.6 cm * 80 cm posts, with the distilled water balance; The fig polysaccharide that step (2) is obtained is dissolved in deionized water, the polysaccharide soln that compound concentration is 15mg/mL, on Sephacryl S-300HR molecular sieve column that balance is good, applied sample amount is 2mL, with deionized water, with the flow velocity of 6 mL/h, carries out wash-out; Collected with fraction collector, surveyed the total sugar concentration of every pipe by the phenolsulfuric acid colorimetry, then merged identical component according to elution curve, after the dialysis of desalting, 40 ℃ of concentrating under reduced pressure postlyophilizations; As above operation is repeatedly carried out the lyophilize of purifying several times and is obtained the fig polysaccharide sterling.
Below the inventive method is made a more detailed description.
1.1 material and reagent
The Fructus Fici dry fruit is purchased from Hai Lianlu market, Lianyungang; Alpha-glucosidase (lot number: EC3. 2. 1. 20); 4-nitrophenol-α-D-glucopyranoside (PNPG, lot number: 026K1516); Acarbose (lot number: lot 16869); Below all purchased from Sigma company; 4-nitrophenols (PNP, Shanghai brilliant pure reagent company limited); Sulfuric acid, glucose, phenol, ethanol etc. are domestic analytical pure.
2 methods
2.1 preparation and the assay of Fructus Fici Crude polysaccharides (CFCP)
2.1.1 typical curve
Adopt with glucose as a standard product of phenol-sulfuric acid process, absorbancy at 490nm colorimetric estimation polysaccharide extraction liquid, carry out linear regression with absorbancy (A) and solution polysaccharide mass concentration (c), obtain regression equation and be: A=14.568c – 0.0082, r=0.9972.Result shows, glucose is good at 7.5～52.5 μ g/mL and absorbancy linear relationship.
2.1.2 CFCP extracts
Due to defects such as classical water extracting method are consuming time, and yield is low, in fig polysaccharide is slightly put forward process, adopt microwave method to extract, for obtaining maximum polysaccharide yield, the optimization that has adopted the response surface method to be extracted each factor.Adopt four factors larger on fig polysaccharide yield impact, added pure volume multiple when microwave power, microwave time, solid-liquid ratio and alcohol precipitation.
Pre-treatment before extracting: get that pulled figs is some to be shredded, with 95% alcohol reflux degrease and pigment, after suction filtration, residue removes small molecular weight impurity with 70% alcohol reflux, then gets residue, as the extraction raw material of fig polysaccharide.After getting the residue water and extracting under microwave condition as solvent, suction filtration, get filtrate and be concentrated into the 100mL left and right with rotary evaporation, add standing 24h after several times volume 95% ethanol, get precipitation after centrifugal, precipitation by the Sevage method except after Deproteinization, lyophilize, obtain Fructus Fici Crude polysaccharides CFCP.And be mixed with certain density CFCP solution, stand-by.
Table 1 fig polysaccharide extracts response surface analysis factor and level
Table 2 Box-Behnken design and response value
The regression equation gone out by the Box-Behnken experimental fit is: Y=10.0332+0.1688X
1+ 0.3285X
2+ 0.4118X
3-0.2475X
4-0.2950X
1 2-1.0071X
2 2-0.7270X
3 2-0.7709X
4 2+ 0.2498X
1x
2-0.0878X
1x
3-0.1013X
1x
4+ 0.6615X
2x
3-0.3713 X
2x
4-0.3510X
3x
4
In formula, Y is polysaccharide yield (%), X
1for liquid ratio (g/mL), X
2for microwave power (W), X
3for volume fraction of ethanol (%), X
4for extraction time (min).
The every variance analysis of regression equation is in Table 3.
Table 3 analysis of variance table
Annotate: * * Pr to be less than 0.0001 be highly significant to F value; * Pr > the F value is less than 0.05 for significantly
Can find out, while with above-mentioned regression equation, describing concerning between each factor and response value, the linearity between dependent variable and all independent variable(s)
Relation is (r=17.75/18.05=0.9834) significantly, and the conspicuous level of model is far smaller than 0.05, and now Quadratic regression variance model is highly significant.The results of analysis of variance every from regression equation it can also be seen that, it is less that the mistake of equation is intended, and shows that this equation is good to the experimental result matching, and experimental error is little, and therefore, available this regression equation replaces the true point of experiment to carry out analysis and prediction to experimental result.
To regression equation go single order partially inverse equal 0, arrange to obtain response value when maximum, X
1=0.49, X
2=0.50, X
3=0.59, X4=-0.45.The optimum process condition that corresponding fig polysaccharide extracts is: solid-liquid ratio 1:22.45, microwave power 550W, extraction time 12.9min, alcohol precipitation is extracting solution by amount of alcohol 3.55 times, with this understanding, the yield of fig polysaccharide can reach 10.33% in theory.For the ease of operation, optimum extraction condition is modified to: solid-liquid ratio 1:20, microwave power is 550W, and extraction time is 13 minutes, and alcohol precipitation ethanol volume multiple is 3.5 times.Adopt above-mentioned optimal conditions, carried out extracting the confirmatory experiment of fig polysaccharide, parallel 3 times, the average extraction yield obtained is 10.24%, with predictor, substantially conforms to, and illustrates that the response surface method is feasible to the optimization of fig polysaccharide extraction conditions.
The fig polysaccharide yield that has compared microwave extraction method and traditional water extraction.Water extraction 2h consuming time, extract 2 times as a result, and yield is 8.87%, illustrates that microwave method extracts polysaccharide, with respect to traditional water extraction have consuming time less, energy-conservation, yield advantages of higher.Polysaccharide content in Crude polysaccharides is high, not only can take full advantage of raw material, can also make follow-up purifying work more easy to operate.
2.1.3CFCP middle determination of polysaccharide
Get the CFCP52.0124mg after lyophilize, be dissolved in the 100mL volumetric flask, and be adjusted to scale with pure water.After shaking up, precision is drawn 5mL in the 10mL volumetric flask, and mends to scale with pure water.Shake up, get 1mL and carry out quantitative assay, average three times, obtain A=0.23, bringing typical curve into, to obtain concentration in colorimetric cylinder be 0.01635ug/mL.So in FCP, polysaccharide content is 0.01635 * 10 * 1 * 10 * 20=32.7018mg.So in CFCP, polysaccharide content is 32.7018/52.0124 * 100%=62.76%.
2.2 the purifying of CFCP
For obtaining the fig polysaccharide that purity is higher, further adopt column chromatography to carry out purifying.
2.2.1 ion-exchange cellulose DEAE-52 is after the abundant swelling of distilled water, use again the distilled water repetitive scrubbing with the NaOH of 0.5 M and the HCl alternate treatment of 0.5 M for several times, until its pH is filled post (2.0cm * 30 cm normal pressure core glass columns) in 6 left and right, it is stand-by that the ion column installed carries out balance 24 hours with distilled water again.Take distilled water through the Fructus Fici Crude polysaccharides CFCP 1g of dialysis freeze-drying and be mixed with the solution that mass concentration is 1%.Then by DEAE-52 ion exchange column that on fig polysaccharide CFCP solution, balance is good, the loading volume is 5 mL, cross post with 0-1M NaCl 200 mL with the flow velocity of 50 mL/h, with automatic Fraction Collector, with the amount of every pipe 5mL, collected, total sugar concentration with the every pipe of phenolsulfuric acid colorimetric method for determining is made elution curve, collect identical component according to elution curve, collect 13 pipes to 32 pipes, in 40 ℃ of concentrating under reduced pressure lyophilize, obtain fig polysaccharide FCP.Elution curve is shown in Fig. 1.
2.2.2 Sephacryl S-300HR molecular sieve column chromatography
Sephacryl S-300HR softly washs for several times, reduces pressure after degassed through distilled water and fills post (1.6 cm * 80 cm), with the distilled water balance.To be dissolved in deionized water by the resulting fig polysaccharide FCP of ion exchange column, the polysaccharide soln that compound concentration is 15mg/mL, on Sephacryl S-300HR molecular sieve column that balance is good, applied sample amount is 2mL, with deionized water, with the flow velocity of 6 mL/h, carries out wash-out.Collected with fraction collector, surveyed the total sugar concentration of every pipe by the phenolsulfuric acid colorimetry, then merged identical component according to elution curve, collected 27 pipes to 43 pipes, after the dialysis of desalting, 40 ℃ of concentrating under reduced pressure postlyophilizations.As above operation is repeatedly carried out purifying lyophilize several times and is obtained fig polysaccharide sterling FCP1.Elution curve is shown in Fig. 2.
2.2.3 fig polysaccharide FCP1 purity checking
Preparation concentration is 10mg/mL fig polysaccharide sterling FCP solution, gets the upper processed good Sephacryl S-300HR post of 2mL, and elution curve is shown in Fig. 3.Visible elution peak is single symmetrical peak, illustrates that FCP1 is the polysaccharide of character homogeneous.
2.2.4 product characterizes
2.2.4.1 get the solution that sterling FCP1 is mixed with 0.1mg/mL on a small quantity, scanning spectra under ultraviolet, 260nm, 280nm does not absorb, and shows in polysaccharide not containing impurity such as albumen.
2.2.4.2 get fig polysaccharide sample F CP1 2 mg of purifying, by the KBr pressed disc method, in 4000-400 cm-1 interval, scan to obtain infrared spectrogram.See Fig. 4.
Visible in Fig. 4, at 3448cm
-1the stretching vibration absorption peak that the strong absorption peak at place is the alcoholic extract hydroxyl group-OH in polysaccharide molecule, 2940cm
-1the absorption peak of vicinity is the stretching vibration peak of C-H, at 1588cm
-1the absorption peak at place is-the flexural vibration absorption peak of OH.At 1378cm
-1place is C-H flexural vibration absorption peaks.At 1106cm
-1the angle vibration absorption peak of the strong absorption peak proof alcoholic extract hydroxyl group-OH of vicinity.At 846cm
-1there is the charateristic avsorption band of α-type glycosidic link in place, illustrates that this fig polysaccharide is α-polysaccharide.
2.3 alpha-glucosidase suppresses determination of activity
2.3.1 alpha-glucosidase suppresses activity test method
Take PNPG as substrate, and reaction system is: the phosphate buffered saline buffer of 0.1mol/L (pH value 6.8) 0.6mL, alpha-glucosidase 0.1mL, after 37 ℃ of insulation 15min, add 0.05mL testing sample solution and 0.2mL substrate PNPG, 37 ℃ of reaction 15 min, add 1mol/L Na
2cO
3solution 0.5mL termination reaction, measure the A value at 405 nm places.Be calculated as follows inhibiting rate, wherein the sample contrast is only with damping fluid and sample solution, and control group is not containing sample, and blank is only with damping fluid.Positive controls replaces sample with the positive control drug acarbose.
Inhibiting rate P (%)=1-
2.3.2 the mensuration of alpha-glucosaccharase enzyme activity
According to adopted reaction system, the phosphate buffered saline buffer of 0.1mol/L (pH6.8) 0.6mL, alpha-glucosidase 0.1mL, after 37 ℃ of insulation 15min, add 200 μ L PNPG, 37 ℃ of reaction 15 min, then add 1mol/L Na
2cO
3solution 0.5mL termination reaction, measure absorption value at 405 nm places.
Wherein enzyme activity unit definition: at 37 ℃, under pH value 6.8 conditions, in 1min, hydrolysis PNPG discharges the required enzyme amount (U) of 1 μ moL PNP.
2.3.3 the making of typical curve
Prepare 1000 μ moL/L with phosphate buffered saline buffer (pH 6.8)
-1pNP solution, distinguish accurate absorption 2,1.75,1.5,1.25,1,0.75,0.5,0mL to colorimetric cylinder, and add respectively 1 mol/L Na
2cO
3solution 0.5mL, be settled to 5mL, mixes, and the final concentration that makes PNP is 400,350,300,250,200,150,100,50 and 0 μ molL
-1.Measure the A value under 405nm, survey 3 groups and average.Take the A value as ordinate zou, and PNP concentration is X-coordinate, makes typical curve.
2.3.4 FCP suppresses determining of type to alpha-glucosidase
Adding substrate under certain inhibitor concentration condition, its concentration is 0,0.5,1.0,1.5,2.5,4mmol/L, measures enzyme activity.Change inhibitor concentration, be respectively 0,4.3482mg/mL, can obtain the enzyme activity under a series of different concentration of substrate conditions.Press the Lineweave-Burk mapping and determine the inhibition type.
3 results and discussion
3.1 typical curve
The PNP concentration (X) of take is X-coordinate, and the A value is ordinate zou (Y), and the drawing standard curve obtains regression equation Y=0.0022X-0.0088 (r=0.9987), and linear relationship is good, and linear concentration range is 0 ~ 400 μ molL
-1.
3.2 the restraining effect of different concns FCP to alpha-glucosidase
Experimental result shows, along with the rising of FCP concentration, suppresses activity and improves thereupon.And to take FCP concentration (mg/mL) be X-coordinate, inhibiting rate (%) is ordinate zou, draws Trendline, and obtains regression equation Y=9.8746X+2.8243(r=0.9985), calculating 503nhibiting concentration is Ic
50=4.7775mg/mL.
3.6 suppress determining of type
3.6.1 the mensuration of km value
The amount of the PNP that speed of reaction (V) can produce with hydrolysis substrate in the unit time mean, in experiment, speed of reaction is tried to achieve by the light absorption value of the PNP that generates in the unit time in the assaying reaction system.
Regression equation is: Y=39.697X+29.921 (r=0.9580) can be obtained by formula, and km is 1.2599 * 10
-3moL/L, maximum reaction velocity is 33.42moL/Lmin.
3.6.2 suppressing type determines
Known according to figure, speed of reaction Vmax diminishes with the increase of inhibitor concentration, and Michaelis-Menton constant Km remains unchanged, and therefore infers, FCP belongs to the noncompetitive inhibition to the restraining effect of alpha-glucosidase inhibitor.
The accompanying drawing explanation
The DEAE-52 column chromatography elution curve that Fig. 1 is Fructus Fici Crude polysaccharides CFCP;
The Sephacryl S-300HR column chromatography elution curve that Fig. 2 is fig polysaccharide FCP;
The Sephacryl S-300HR column chromatography elution curve that Fig. 3 is fig polysaccharide FCP1;
The infrared spectrogram that Fig. 4 is fig polysaccharide FCP1.
Embodiment
Below further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not form the restriction to its right.
(1) ion-exchange cellulose DEAE-52 is after the abundant swelling of distilled water, use again the distilled water repetitive scrubbing with the NaOH of 0.5 M and the HCl alternate treatment of 0.5 M for several times, until its pH to 6 is filled post, adopt 2.0cm * 30 cm normal pressure core glass columns, it is stand-by that the ion column installed carries out balance 24 hours with distilled water again;
(2) getting the Fructus Fici Crude polysaccharides take distilled water and is mixed with the solution that mass concentration is 1%; Then by DEAE-52 ion exchange column that on fig polysaccharide solution, balance is good, the loading volume is 5 mL, cross post with 0-1M NaCl 200 mL with the flow velocity of 50 mL/h, with automatic Fraction Collector, with the amount of every pipe 5mL, collected, total sugar concentration with the every pipe of phenolsulfuric acid colorimetric method for determining is made elution curve, collect identical component (collecting 13 pipes to 32 pipes) according to elution curve, in 40 ℃ of concentrating under reduced pressure lyophilize, obtain fig polysaccharide;
(3) Sephacryl S-300HR softly washs for several times, reduces pressure after degassed through distilled water and fills post, adopts 1.6 cm * 80 cm posts, with the distilled water balance; The fig polysaccharide that step (2) is obtained is dissolved in deionized water, the polysaccharide soln that compound concentration is 15mg/mL, on Sephacryl S-300HR molecular sieve column that balance is good, applied sample amount is 2mL, with deionized water, with the flow velocity of 6 mL/h, carries out wash-out; Collected with fraction collector, surveyed the total sugar concentration of every pipe by the phenolsulfuric acid colorimetry, then merged identical component (collecting 27 pipes to 43 pipes) according to elution curve, after the dialysis of desalting, 40 ℃ of concentrating under reduced pressure postlyophilizations; As above operation is repeatedly carried out the lyophilize of purifying several times and is obtained the fig polysaccharide sterling.
The fig polysaccharide sterling that the Fructus Fici Crude polysaccharides that embodiment 1 or 2 or 3 described methods make or the described purification process purifying of embodiment 4 obtain can be for the preparation of alpha-glucosidase inhibitor.
Claims (4)
1. the preparation method of a Fructus Fici Crude polysaccharides, is characterized in that, its step is as follows: get that pulled figs is some to be shredded, with 95% alcohol reflux degrease and pigment, after suction filtration, residue removes small molecular weight impurity with 70% alcohol reflux; Then get residue, as the extraction raw material of fig polysaccharide; Get residue, after water extracts under microwave condition as solvent, suction filtration, get filtrate and be concentrated into 100Ml with rotary evaporation, adds standing 24h after 95% ethanol, gets precipitation after centrifugal, and precipitation is by the Sevage method except after Deproteinization, and lyophilize, obtain the Fructus Fici Crude polysaccharides; During extraction, the solid-liquid ratio 1:20-23 of residue and water; Microwave power is 550W, and extraction time is 13min, and the 3.50-3.60 that alcohol precipitation is extracting liquid volume by amount of alcohol doubly.
2. the preparation method of Fructus Fici Crude polysaccharides according to claim 1 is characterized in that: during extraction, and the solid-liquid ratio 1:22.45 of residue and water; Alcohol precipitation is extracting liquid volume by amount of alcohol 3.55 times.
3. the purification process of the Fructus Fici Crude polysaccharides that method makes as claimed in claim 1 or 2, is characterized in that, its step is as follows:
(1) ion-exchange cellulose DEAE-52 is after the abundant swelling of distilled water, use again the distilled water repetitive scrubbing with the NaOH of 0.5 M and the HCl alternate treatment of 0.5 M for several times, until its pH to 6 is filled post, adopt 2.0cm * 30 cm normal pressure core glass columns, it is stand-by that the ion column installed carries out balance 24 hours with distilled water again;
(2) getting the Fructus Fici Crude polysaccharides take distilled water and is mixed with the solution that mass concentration is 1%; Then by DEAE-52 ion exchange column that on fig polysaccharide solution, balance is good, the loading volume is 5 mL, cross post with 0-1M NaCl 200 mL with the flow velocity of 50 mL/h, with automatic Fraction Collector, with the amount of every pipe 5mL, collected, total sugar concentration with the every pipe of phenolsulfuric acid colorimetric method for determining is made elution curve, collect identical component according to elution curve, in 40 ℃ of concentrating under reduced pressure lyophilize, obtain fig polysaccharide;
(3) Sephacryl S-300HR softly washs for several times, reduces pressure after degassed through distilled water and fills post, adopts 1.6 cm * 80 cm posts, with the distilled water balance; The fig polysaccharide that step (2) is obtained is dissolved in deionized water, the polysaccharide soln that compound concentration is 15mg/mL, on Sephacryl S-300HR molecular sieve column that balance is good, applied sample amount is 2mL, with deionized water, with the flow velocity of 6 mL/h, carries out wash-out; Collected with fraction collector, surveyed the total sugar concentration of every pipe by the phenolsulfuric acid colorimetry, then merged identical component according to elution curve, after the dialysis of desalting, 40 ℃ of concentrating under reduced pressure postlyophilizations; As above operation is repeatedly carried out the lyophilize of purifying several times and is obtained the fig polysaccharide sterling.
4. the purposes of fig polysaccharide sterling in preparing alpha-glucosidase inhibitor that the Fructus Fici Crude polysaccharides that method makes as claimed in claim 1 or 2 or purification process purifying as claimed in claim 3 obtain.
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| CN113880962A (en) * | 2021-11-15 | 2022-01-04 | 新疆前进荣耀投资有限公司 | Fig polysaccharide and extraction method and application thereof |
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