CN103344642A - Full-automatic external detection equipment and method for measuring specific egg white by turbidimetry - Google Patents
Full-automatic external detection equipment and method for measuring specific egg white by turbidimetry Download PDFInfo
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- CN103344642A CN103344642A CN2013102818566A CN201310281856A CN103344642A CN 103344642 A CN103344642 A CN 103344642A CN 2013102818566 A CN2013102818566 A CN 2013102818566A CN 201310281856 A CN201310281856 A CN 201310281856A CN 103344642 A CN103344642 A CN 103344642A
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Abstract
The invention discloses full-automatic external detection equipment and method for measuring specific egg white by turbidimetry. The full-automatic external detection equipment comprises a detecting platform, a mechanical arm and a loading pump which are arranged on the detecting platform, a sample adding needle on the detecting platform, and a washing device which is arranged on the detecting platform and is used for washing the sample adding needle, wherein the detecting platform is provided with a plurality of photoelectric detection modules with temperature control and stirring functions, and the photoelectric detection modules are provided with a plurality of reaction cups; the loading pump is connected with the sample adding needle. The full-automatic external detection equipment and method provided by the invention have the advantages that the full-automatic loading of a first detection reagent, a sample to be detected and a second detection reagent are realized, so that the manual loading operation is omitted, the labor cost of the external detection is saved, and a condition that faults in an operation of manually and dropwise adding the detection reagent influence the detection result is also avoided as far as possible; meanwhile, the detection efficiency is improved, the detection time is shortened, and thus the full-automatic external detection equipment has wide market prospect.
Description
Technical field
The present invention relates to in-vitro diagnosis equipment, in particular a kind of full-automatic vitro detection Apparatus and method for that adopts turbidimetry to measure specific protein.
Background technology
So-called specific protein refers to have certain physiological function in the body, plays those specific proteins of important pathology sense when morbid state again.By these specific proteins being detected the physiological situation that to determine patient's body usually.Existing specific protein can be used for following clinical detection: immune dysfunction (detecting IgA, IgG, IgM, complement C3, complement C4, κ light chain, lambda light chain); Inflammatory conditions monitoring (detecting C-reactive protein, α acidoglycoprotein, haptoglobin, CER); The monitoring of rheumatism (detection type rheumatism factor R F, antistreptolysin O (ASO), C-reactive protein) cardiovascular function (detects super quick C-reactive protein, aPoA 1, apolipoprotein B, lipoprotein a); Renal function detects (detecting microdose urine protein, transferrins, α 1-microglobulin, β2Wei Qiudanbai, IgG); Nutrition condition monitoring (detecting albumin, prealbumin, transferrins); Blood disease (detecting antithrombin, transferrins, haptoglobin); The nervous system disease (detecting albumin, alpha2 Macroglobulin, IgA, IgG, IgM) etc.
At present, the common methods of measuring specific protein is turbidimetry, for example scattering turbidimetry method or turbidimetry, this method is that detected sample is added to earlier among the reagent R1, reaction a period of time adds reagent R2 again, after reacting a period of time again, the turbidity of measuring reaction product changes, and determines the content of specific protein in the detected sample.
Existing equipment based on turbidimetry detection specific protein has two kinds, a kind of is large automatic Biochemical Analyzer, usually be used in clinical laboratory or the centralab of big-and-middle-sized hospital, another kind of small-sized semi-automatic checkout equipment is used in general clinical department or She Kang center.
Small-sized semi-automatic specific protein checkout equipment as shown in Figure 1 at present, monitor station 10 is provided with the Photoelectric Detection module 20 of a plurality of band temperature controls and agitating function, the testing staff is equipped with detected sample S in the reaction cup 21 that detects reagent R1 manual the adding in advance during measurement, again reaction cup 21 is inserted in the Photoelectric Detection module 20, two kinds of materials are reacted a period of time in reaction cup, and then will detect in the reagent R2 adding reaction cup 21, react a period of time again, measure the turbidity of reaction product in the reaction cup by the microcontroller of checkout equipment, thereby calculate the content of specific protein in the detected sample.Yet this detection mode exists following defective:
1) detecting reagent R2 needs to add manually, because reaction cup is the upper end tapered opening, the lower end is chamber, Vertical Narrow footpath, manually splash into when detecting reagent, very easily because testing staff's misoperation, part is detected on the inwall that reagent drops in reaction cup, make the quantity not sufficient of the detection reagent of participating in reaction, and then influence testing result;
2) reaction needed a period of time of detection reagent R1 and detected sample, the instrument alert adds when detecting reagent R2, because detecting the speed of reagent, each operating personnel's skilled operation degree or adding can have nothing in common with each other, therefore, it is different that this can make detection reagent and the reaction time of detected sample exist, and causes between the testing result also can there are differences; Simultaneously, the mode of this artificial interpolation has increased testing staff's workload, and is prone to monitoring error and influences testing result.
3) addition of detection reagent R2 generally is several microlitres, and operating personnel are had very high technical ability requirement, and careless slightly will causing detected the reagent dosage deficiency, very easily produces error, thereby influences the accuracy of measurement result.
4) detect reagent R1 and be contained in advance in the reaction cup, long storage time can cause the variation of its composition, for example, volatilization or react with air and to cause that composition changes etc., these all can influence the testing result of specific protein
Therefore, prior art has yet to be improved and developed.
Summary of the invention
At the above-mentioned defective of prior art, the invention provides a kind of full-automatic vitro detection Apparatus and method for that adopts turbidimetry to measure specific protein, realize detecting the automatic loading of reagent.And then be the robotization that realizes that specific protein detects.
The technical scheme that technical solution problem of the present invention adopts is as follows:
A kind of full-automatic vitro detection equipment that adopts turbidimetry to measure specific protein, wherein, comprise a monitor station, described monitor station be provided with band temperature control and agitating function the Photoelectric Detection module and, be placed with reaction cup at described Photoelectric Detection module, also comprise the mechanical arm and the load pump that are arranged on the described monitor station, and be installed in the sample needle on the described mechanical arm, described load pump is connected with described sample needle; Also comprise the cleaning device that is arranged on the described monitor station and is used for cleaning described sample needle, and the microcontroller of controlling described checkout equipment testing process.
Described employing turbidimetry is measured the full-automatic vitro detection equipment of specific protein, and wherein, described Photoelectric Detection module includes several.
Described employing turbidimetry is measured the full-automatic vitro detection equipment of specific protein, wherein, described Photoelectric Detection module on monitor station linearly or circular arc arrange.
Described employing turbidimetry is measured the full-automatic vitro detection equipment of specific protein, wherein, be arranged side by side first container that holds detected sample and hold second at described monitor station and described Photoelectric Detection module and detect second container of reagent and/or hold first the 3rd container that detects reagent.
Described employing turbidimetry is measured the full-automatic vitro detection equipment of specific protein, and wherein, described cleaning device comprises a scavenging pump, connects load pump and the 4th container that holds cleaning reagent respectively.
Described employing turbidimetry is measured the full-automatic vitro detection equipment of specific protein, and wherein, described load pump is fixed displacement pump.
Described employing turbidimetry is measured the full-automatic vitro detection equipment of specific protein, and wherein, described fixed displacement pump is ram pump or syringe pump.
A kind of full-automatic external detection method that adopts turbidimetry to measure specific protein wherein, when prepackage first detects reagent in reaction cup, comprises step:
The microprocessor controls sample needle moves along mechanical arm, and first container that holds detected sample is aimed at and be inserted into to sample needle;
The start-up loading pump is drawn detected sample by sample needle, and mobile sample needle adds detected sample in the reaction cup in reaction cup, and by cleaning device sample needle is carried out cleaning operation;
When detecting the first detection reagent and the reaction time of detected sample in reaction cup then, microcontroller is controlled sample needle again and is moved, and sample needle is aimed at and is inserted into second container that holds the second detection reagent;
Draw second by sample needle and detect reagent, and mobile sample needle detects reagent with second and joins in the reaction cup, and by cleaning device sample needle is carried out cleaning operation in reaction cup;
By the turbidity of reactive material in the detection reaction cup, determine the content of specific protein in the detected sample.
A kind of full-automatic external detection method that adopts turbidimetry to measure specific protein wherein, when not pre-installing the first detection reagent in reaction cup, comprises step:
The microprocessor controls sample needle moves along mechanical arm, sample needle is aimed at and is inserted into the 3rd container that holds the first detection reagent, the start-up loading pump, draw first by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with first and add in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
The microprocessor controls sample needle moves, first container that holds detected sample is aimed at and be inserted into to sample needle, the start-up loading pump, draw detected sample by sample needle, and mobile sample needle is in reaction cup, detected sample is added in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
When detecting the first detection reagent and the reaction time of detected sample in reaction cup then, microcontroller is controlled sample needle again and is moved, sample needle is aimed at and is inserted into second container that holds the second detection reagent, draw second by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with second and join in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
By the turbidity of reactive material in the detection reaction cup, determine the content of specific protein in the detected sample.
A kind of full-automatic external detection method that adopts turbidimetry to measure specific protein wherein, when not pre-installing the first detection reagent in reaction cup, comprises step:
The microprocessor controls sample needle moves along mechanical arm, first container that holds detected sample is aimed at and be inserted into to sample needle, the start-up loading pump, draw detected sample by sample needle, and mobile sample needle is in reaction cup, detected sample is added in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
The microprocessor controls sample needle moves, sample needle is aimed at and is inserted into the 3rd container that holds the first detection reagent, the start-up loading pump, draw first by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with first and add in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
When detecting the first detection reagent and the reaction time of detected sample in reaction cup then, microcontroller is controlled sample needle again and is moved, first container that holds detected sample is aimed at and be inserted into to sample needle, draw second by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with second and join in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
By the turbidity of reactive material in the detection reaction cup, determine the content of specific protein in the detected sample.
Employing turbidimetry provided by the present invention is measured the full-automatic vitro detection Apparatus and method for of specific protein, its technical advantages that reaches is: by the setting of mechanical arm, load pump and sample needle, realize detecting the automatic loading of reagent and detected sample, save the cost of labor that specific protein detects, the operation error of having avoided artificial dropping to detect reagent or detected sample impacts testing result.
Description of drawings
Fig. 1 is the structural representation of the vitro detection equipment of prior art.
Fig. 2 is the structural representation that employing turbidimetry provided by the invention is measured the full-automatic vitro detection equipment of specific protein.
Fig. 3 is the structural representation that employing turbidimetry provided by the invention is measured reaction cup in the full-automatic vitro detection equipment of specific protein.
Fig. 4 is the structural representation that employing turbidimetry provided by the invention is measured Photoelectric Detection module in the full-automatic vitro detection equipment of specific protein.
Fig. 5 is the structural representation of the another preferred embodiment of the employing turbidimetry provided by the invention full-automatic vitro detection equipment of measuring specific protein.
Fig. 6 is that employing turbidimetry provided by the invention is measured in the full-automatic external detection method of specific protein and loaded the process flow diagram that detected sample and second detects reagent automatically.
Fig. 7 is that employing turbidimetry provided by the invention is measured and loaded first in the full-automatic external detection method of specific protein automatically and detect the process flow diagram that reagent, detected sample and second detect an embodiment of reagent.
Fig. 8 is that employing turbidimetry provided by the invention is measured and loaded first in the full-automatic external detection method of specific protein automatically and detect the process flow diagram that reagent, detected sample and second detect the another embodiment of reagent.
Embodiment
For making purpose of the present invention, technical scheme and advantage clearer, clear and definite, below develop simultaneously with reference to accompanying drawing that the present invention is described in more detail for embodiment.Should be appreciated that specific embodiment described herein only in order to explaining the present invention, and be not used in restriction the present invention.
The invention provides a kind of full-automatic vitro detection equipment that adopts turbidimetry to measure specific protein, as shown in Figure 2, comprise a monitor station 100, described monitor station 100 is provided with the Photoelectric Detection module 110 of band temperature control and agitating function, and be placed with reaction cup 200 at Photoelectric Detection module 110, reaction cup 200 is used for prepackage first and detects reagent and detect reagent as first, detected sample and second detects the reaction vessel of reagent, this checkout equipment comprises that also one is arranged on mechanical arm 101 and the load pump 102 on the described monitor station 100, and a sample needle 103, that is installed on the described mechanical arm 101 is arranged on the cleaning device 150 that also is used for cleaning described sample needle 103 on the described monitor station 100.Mechanical arm 101 is fixed on the monitor station 100 by screw 140, and sample needle 103 can move along mechanical arm 101, and is connected with load pump 102, and this load pump 102 can make sample needle draw and detect reagent or detected sample.Load pump 102 is connected by conduit 120 with sample needle 103 and container, but is not limited to conduit.
This checkout equipment also comprises and is arranged on first container 104 that holds detected sample on the monitor station and holds second second container 105 that detects reagent.
Further, checkout equipment provided by the invention can detect for single channel, can be multi-channel detection, single channel only arranges a luminous point and detects module on monitor station when detecting, and during multi-channel detection, present embodiment includes several Photoelectric Detection modules, and this moment, the Photoelectric Detection module was arranged in a linear at monitor station, the quantity of Photoelectric Detection module is arranged as required, usually but be not limited to 4 to 8, the modes of emplacement of this straight-line has clearly advantage with respect to existing circular modes of emplacement.Reaction cup structural representation as shown in Figure 3, reaction cup 200 is generally the upper end pyramidal structure, opening is square, the lower end is vertical cavity, vertically cavity diameter is less, these reaction cup are placed on the Photoelectric Detection module, if adopt circular the placement, the placement orientation difference of each reaction cup then, splashing in the process that detects reagent or detected sample to one of them, be easy to cause detection reagent or detected sample to be dropped on the inwall of reaction cup, influencing testing result, and circular structure of placing is complicated, and cost is higher.Can reduce as much as possible and detect reagent or detected sample and dropped in probability on the reaction cup inwall, reduce the error risk and adopt straight-line that the Photoelectric Detection module is set.Simultaneously, straight-line arranges the control that the Photoelectric Detection module also can be simplified sample needle greatly.Certainly, the Photoelectric Detection module is not limited to adopt line spread at monitor station, also can adopt circular arc to arrange or other arrangement modes.
Preferably, the structure of Photoelectric Detection module of the present invention as shown in Figure 4, this Photoelectric Detection module comprise a light source 111 that is arranged on reaction cup 200 1 sides, be arranged on reaction cup 200 opposite sides be the photoelectric sensor 113 of predetermined angular with light source over against the optical filter 112 that arranges with the light horizontal direction of living in that light source is launched, the angle of photoelectric sensor and level is α, the big I of α is adjusted according to actual needs, preferable range is [0 °, 30 °].Described light source is used for sending the detection light beam, and described optical filter 112 is used for the detection light beam through reaction cup scattering or transmission is filtered, and described photoelectric sensor 113 is used for receiving the detection light beam after optical filter filters.Detection light beam irradiates detection reagent and detected sample fully react the reaction cup after finishing, owing to contain certain particle in suspension in the reactive material in the reaction cup, when the detection light beam contains the medium of particle in suspension by one, because particle in suspension is to scattering of light effect and optionally absorption, the transmission light intensity is weakened or the scattered intensity enhancing, by the detection to transmitted light or scattered intensity, determine the turbidity of reactive material, and then the content of material composition in the mensuration detected sample, realize the detection of specific protein content.According to the difference of deciding albumen, realize the detection of different test indexs.
The arrangement mode of the structure of mechanical arm of the present invention and Photoelectric Detection module is adaptive, in the present embodiment, arrange because the Photoelectric Detection module is orthoscopic, therefore mechanical arm 101 of the present invention is preferably linear structure, and this linear structure can be simplified the movement of sample needle on mechanical arm.Mechanical arm can be arranged on but be not limited to the Photoelectric Detection module directly over, directly over being preferably, like this, sample needle just can be aimed at reaction cup, is convenient to detect the interpolation of reagent.
Again referring to Fig. 1, meeting of the present invention arranges a microcontroller 130 with timing function at the monitor station of vitro detection checkout equipment, this microcontroller 130 is used for the testing process of the whole checkout equipment of control, concrete for control sample needle 103 is mobile at mechanical arm 101 automatically, and the control load pump is drawn second and is detected reagent, detected sample or the first detection reagent, also controls cleaning device simultaneously to the cleaning of sample needle.It is mobile between reaction cup and first container, second container or the 3rd container that microcontroller is constantly controlled sample needle, finish the automatic loading that the first detection reagent, detected sample and second is detected reagent, can also carry out timing to first reaction time of detecting reagent and detected sample simultaneously, the reaction time then, moved along mechanical arm by the microprocessor controls sample needle, while start-up loading pump, from corresponding container, draw detected sample box second and detect reagent, and by sample needle they are joined in the reaction cup.Present embodiment is controlled first and is detected the uptake that reagent and second detects reagent by the microprocessor controls load pump, so that the testing staff is according to detecting the needs adjustment.
In order to realize that well detected sample or second detects the automatic loading of reagent, the present invention will hold first container 104 of detected sample and hold second second container 105 that detects reagent and be arranged side by side on monitor station 100 with Photoelectric Detection module 101, the both sides that the position can be placed on Photoelectric Detection module 110 for first container 104 and second container 105 specifically are set, perhaps are arranged on same one side of Photoelectric Detection module 110.At this moment, be equipped with first in the reaction cup 200 in advance and detect reagent.When carrying out the automatic loading of detected sample and the second detection reagent, at first, the microprocessor controls sample needle moves along mechanical arm like this, sample needle is aimed at and inserted first container that holds detected sample; Secondly, the start-up loading pump detects reagent from draw detected sample or second by sample needle; Moreover, microcontroller is controlled sample needle again and is moved, sample needle is moved to the reaction cup place, and aim at and react rim of a cup, detected sample or second is detected reagent to splash in the reaction cup, finish the automatic loading that detected sample or second detects reagent, in this process, the intact a kind of reagent of every loading all needs by cleaning device sample needle to be cleaned.
Preferably, in order to realize that first detects reagent, detected sample and second detects loading fully automatically of reagent, the present invention is on above-mentioned technical foundation, as shown in Figure 5, to hold first the 3rd container 106 that detects reagent also with Photoelectric Detection module 101 and be arranged on the monitor station 100, the both sides that the position can be placed on Photoelectric Detection module 110 for first container 104, second container 105 and the 3rd container 106 specifically are set, perhaps be arranged on same one side of Photoelectric Detection module 110, be empty this moment in the reaction cup, do not add any reagent.When carrying out the automatic loading of the first detection reagent, the second detection reagent and detected sample, the microprocessor controls sample needle moves along mechanical arm, at first move to the 3rd container place, the start-up loading pump, from the 3rd container, draw first and detect reagent, again sample needle is moved to the reaction cup place, detect reagent with first and join in the reaction cup; And then sample needle moved to the first container place, in first container, draw detected sample, again sample needle is moved to the reaction cup place, detected sample is joined in the reaction cup, beginning first detects the reaction of reagent and detected sample, after reaction finishes, microcontroller is controlled sample needle again and is moved to the second container place, from second container, draw second and detect reagent, and again sample needle is moved to the reaction cup place, detect reagent with second and join in the reaction cup, finish whole automatic loading procedure.In this automatic loading procedure, only need to be moved along mechanical arm by the microprocessor controls sample needle, and detect automatic absorption and the loading of reagent or detected sample, whole process full automation control, need not manually participate in, save the detection cost greatly, improved detection efficiency, and reduced the detection error that human factor produces.Simultaneously, deposit because the second detection reagent need freeze, therefore, second container of present embodiment is preferably and is positioned in the refrigerating plant.
Load pump of the present invention is fixed displacement pump, and for example ram pump or syringe pump specifically can adopt the high precision injection measurement pump of valveless ram pump or T-valve.Valveless ram pump inside is stupalith, when drawing detection reagent by it detection reagent is sticked in the load pump, influences the subsequent detection result in order to avoid detect for a long time; And the high precision injection measurement pump configuration of T-valve is more complicated, it can at first will detect reagent by a valve and draw in pump, and then discharge detection reagent by another valve, this kind load pump can accurately be controlled the addition that detects reagent, if adopt the high precision injection measurement pump of this T-valve, checkout equipment of the present invention can realize detecting adding more fast and accurately of reagent.
Preferably, cleaning device of the present invention 150 be used for sample needle 103 finish detect reagent or detected sample or second and detect the dropwise operation of reagent first after, sample needle 103 is cleaned, and holding waste liquid after the cleaning by special container, this cleaning device 150 is by microcontroller 130 controls.Particularly, this cleaning device 150 comprises a scavenging pump 151, and this scavenging pump 151 links to each other with the 4th container 153 that holds cleaning reagent respectively at load pump 102, also is provided with a switch valve 152 between this scavenging pump 151 and the load pump 102, and this scavenging pump can be vacuum pump, or the pump of other types.In the specific implementation, sample needle is finished and is detected reagent to first, after detected sample or second detects the dropwise operation of reagent, the microprocessor controls sample needle moves along mechanical arm, sample needle is moved to the cleaning device place, microcontroller is opened switch valve, and startup scavenging pump, from the 3rd container that holds cleaning reagent (for example water), draw cleaning reagent, and cleaning reagent injected load pump, load pump is injected sample needle with cleaning reagent again, cleaning reagent flows out from sample needle, and the waste liquid of outflow can be contained in the special container 153, finishes sample needle is cleaned.The setting of this cleaning device can be preserved the cleaning of sample needle as much as possible, avoids the mixing between all ingredients or because not using residual reagent in it for a long time, operation impacting to subsequent aspiration, and then influences testing result.The adaptive setting of cleaning device of the present invention and mechanical arm is so that sample needle can arrive the cleaning device place along mechanical arm.
Based on above-mentioned checkout equipment, the present invention also provides a kind of full-automatic external detection method that adopts turbidimetry to measure specific protein, as shown in Figure 6, this embodiment is when the first detection reagent is housed in reaction cup in advance, automatically load the implementation step of detected sample and the second detection reagent, comprise step:
Step S101, microprocessor controls sample needle move along mechanical arm, and first container that holds detected sample is aimed at and be inserted into to sample needle;
Step S102, start-up loading pump are drawn detected sample by sample needle, and mobile sample needle adds detected sample in the reaction cup in reaction cup, and by cleaning device sample needle are carried out cleaning operation;
Step S103, detect reagent and the reaction time of detected sample in reaction cup then when detecting first, microcontroller is controlled sample needle again and is moved, and sample needle is aimed at and is inserted into hold second second container that detects reagent;
Step S104, draw second by sample needle and detect reagent, and mobile sample needle detects reagent with second and joins in the reaction cup, and by cleaning device sample needle is carried out cleaning operation in reaction cup;
Step S105, by the turbidity of reactive material in the detection reaction cup, determine the content of specific protein in the detected sample.
Wherein, the present invention detects loading fully automatically of reagent in order to realize to the first detection reagent, detected sample and second, that is to say that concrete implementation step is when not pre-installing the first detection reagent in reaction cup:
Step S201, microprocessor controls sample needle move along mechanical arm, sample needle is aimed at and is inserted into the 3rd container that holds the first detection reagent, the start-up loading pump, draw first by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with first and add in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
Step S202, microprocessor controls sample needle move, first container that holds detected sample is aimed at and be inserted into to sample needle, the start-up loading pump, draw detected sample by sample needle, and mobile sample needle is in reaction cup, detected sample is added in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
Step S203, detect reagent and the reaction time of detected sample in reaction cup then when detecting first, microcontroller is controlled sample needle again and is moved, sample needle is aimed at and is inserted into second container that holds the second detection reagent, draw second by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with second and join in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
Step S204, by the turbidity of reactive material in the detection reaction cup, determine the content of specific protein in the detected sample.
Certainly, loading first order that detects reagent and detected sample by sample needle keeps away testing process and becomes any influence inadequately, therefore, difference and the above-mentioned first detection reagent that loads earlier, the back loads the embodiment of the second detection reagent, another preferred embodiment of the present invention is to load detected sample earlier, and the back loads first and detects reagent, and concrete implementation process comprises:
Step S301, microprocessor controls sample needle move along mechanical arm, first container that holds detected sample is aimed at and be inserted into to sample needle, the start-up loading pump, draw detected sample by sample needle, and mobile sample needle is in reaction cup, detected sample is added in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
Step S302, microprocessor controls sample needle move, sample needle is aimed at and is inserted into the 3rd container that holds the first detection reagent, the start-up loading pump, draw first by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with first and add in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
Step S303, detect reagent and the reaction time of detected sample in reaction cup then when detecting first, microcontroller is controlled sample needle again and is moved, first container that holds detected sample is aimed at and be inserted into to sample needle, draw second by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with second and join in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
Step S304, by the turbidity of reactive material in the detection reaction cup, determine the content of specific protein in the detected sample.
In order to make the above embodiment of the present invention more clear, below in conjunction with specific protein detect a kind of implementation process that the present invention is measured specific protein content by turbidimetry and be specifically described.
This embodiment carries out CRP by the c reactive protein detection kit and detects.CRP is proportionate as acute phase protein rising amplitude and the infected degree of human body.CRP can be used for the antidiastole of bacterium and virus infections.In surperficial healthy population, the concentration of CRP is dangerous directly related with generation coronary heart disease.To resist people's c reactive protein antibody sandwich on latex particle, can with serum in c reactive protein generation agglutinating reaction, form antigen antibody complex, its turbidity height is directly proportional with CRP content in the serum when a certain amount of antibody exists.By detecting transmitted light or scattered intensity, can calculate the content of CRP in the serum with reference to multiple spot calibration calibration curve.When detecting, first of prepackage 600uL detects reagent R1 in the reaction cup, and the detected sample (serum or whole blood) by sample needle absorption 3uL joins in the reaction cup, the Photoelectric Detection module is with its mixing, and place 37 ℃ of incubation a period of times, sample needle moves the second detection reagent R2 that draws 60uL then, and splashes in the reaction cup, the Photoelectric Detection module is with its mixing, and after hatching 10 seconds, read scattered light intensity A1 in continuous 90 seconds, and then calculate corresponding CRP content.The process of above-mentioned detection CRP content only is used for explaining that the present invention measures the method for specific protein content by turbidimetry, and is not used in this method of restriction, because detection method of the present invention can be applied to the detection of multiple specific protein.Such as α acidoglycoprotein, haptoglobin, aPoA 1 etc.
In sum, a kind of full-automatic vitro detection Apparatus and method for that adopts turbidimetry to measure specific protein provided by the invention.The present invention has realized that first detects loading fully automatically of reagent, detected sample and the second detection reagent, saved the operation of artificial loading, not only save the cost of labor of vitro detection, but also the operation error of having avoided artificial dropping to detect reagent as much as possible impacts to testing result; The present invention has simultaneously improved detection efficiency, has shortened detection time greatly, has vast market prospect.
Should be understood that application of the present invention is not limited to above-mentioned giving an example, for those of ordinary skills, can be improved according to the above description or conversion that all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (10)
1. full-automatic vitro detection equipment that adopts turbidimetry to measure specific protein, it is characterized in that, comprise a monitor station, described monitor station is provided with the Photoelectric Detection module of band temperature control and agitating function, be placed with reaction cup at described Photoelectric Detection module, also comprise the mechanical arm and the load pump that are arranged on the described monitor station, and be installed in the sample needle on the described mechanical arm, described load pump is connected with described sample needle; Also comprise the cleaning device that is arranged on the described monitor station and is used for cleaning described sample needle, and the microcontroller of controlling described checkout equipment testing process.
2. employing turbidimetry according to claim 1 is measured the full-automatic vitro detection equipment of specific protein, it is characterized in that described Photoelectric Detection module includes several.
3. employing turbidimetry according to claim 2 is measured the full-automatic vitro detection equipment of specific protein, it is characterized in that, described Photoelectric Detection module on monitor station linearly or circular arc arrange.
4. employing turbidimetry according to claim 1 is measured the full-automatic vitro detection equipment of specific protein, it is characterized in that, be arranged side by side first container that holds detected sample and hold second at described monitor station and described Photoelectric Detection module and detect second container of reagent and/or hold first the 3rd container that detects reagent.
5. employing turbidimetry according to claim 1 is measured the full-automatic vitro detection equipment of specific protein, it is characterized in that described cleaning device comprises a scavenging pump, connects load pump and the 4th container that holds cleaning reagent respectively.
6. employing turbidimetry according to claim 1 is measured the full-automatic vitro detection equipment of specific protein, it is characterized in that described load pump is fixed displacement pump.
7. employing turbidimetry according to claim 6 is measured the full-automatic vitro detection equipment of specific protein, it is characterized in that described fixed displacement pump is ram pump or syringe pump.
8. a full-automatic external detection method that adopts turbidimetry to measure specific protein is characterized in that, when prepackage first detects reagent in reaction cup, comprises step:
The microprocessor controls sample needle moves along mechanical arm, and first container that holds detected sample is aimed at and be inserted into to sample needle;
The start-up loading pump is drawn detected sample by sample needle, and mobile sample needle adds detected sample in the reaction cup in reaction cup, and by cleaning device sample needle is carried out cleaning operation;
When detecting the first detection reagent and the reaction time of detected sample in reaction cup then, microcontroller is controlled sample needle again and is moved, and sample needle is aimed at and is inserted into second container that holds the second detection reagent;
Draw second by sample needle and detect reagent, and mobile sample needle detects reagent with second and joins in the reaction cup, and by cleaning device sample needle is carried out cleaning operation in reaction cup;
By the turbidity of reactive material in the detection reaction cup, determine the content of specific protein in the detected sample.
9. a full-automatic external detection method that adopts turbidimetry to measure specific protein is characterized in that, when not pre-installing the first detection reagent in reaction cup, comprises step:
The microprocessor controls sample needle moves along mechanical arm, sample needle is aimed at and is inserted into the 3rd container that holds the first detection reagent, the start-up loading pump, draw first by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with first and add in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
The microprocessor controls sample needle moves, first container that holds detected sample is aimed at and be inserted into to sample needle, the start-up loading pump, draw detected sample by sample needle, and mobile sample needle is in reaction cup, detected sample is added in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
When detecting the first detection reagent and the reaction time of detected sample in reaction cup then, microcontroller is controlled sample needle again and is moved, sample needle is aimed at and is inserted into second container that holds the second detection reagent, draw second by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with second and join in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
By the turbidity of reactive material in the detection reaction cup, determine the content of specific protein in the detected sample.
10. a full-automatic external detection method that adopts turbidimetry to measure specific protein is characterized in that, when not pre-installing the first detection reagent in reaction cup, comprises step:
The microprocessor controls sample needle moves along mechanical arm, first container that holds detected sample is aimed at and be inserted into to sample needle, the start-up loading pump, draw detected sample by sample needle, and mobile sample needle is in reaction cup, detected sample is added in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
The microprocessor controls sample needle moves, sample needle is aimed at and is inserted into the 3rd container that holds the first detection reagent, the start-up loading pump, draw first by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with first and add in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
When detecting the first detection reagent and the reaction time of detected sample in reaction cup then, microcontroller is controlled sample needle again and is moved, first container that holds detected sample is aimed at and be inserted into to sample needle, draw second by sample needle and detect reagent, and mobile sample needle is in reaction cup, detect reagent with second and join in the reaction cup, and by cleaning device sample needle is carried out cleaning operation;
By the turbidity of reactive material in the detection reaction cup, determine the content of specific protein in the detected sample.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901204A (en) * | 2014-04-11 | 2014-07-02 | 深圳市儿童医院 | Immunoturbidimetry kit for detecting streptococcus agalactiae and detection method of immunoturbidimetry kit |
| CN104535776A (en) * | 2014-12-05 | 2015-04-22 | 深圳普门科技有限公司 | Immune scatter turbidimetry based full-automatic detection device and method thereof |
| CN105928907A (en) * | 2016-05-17 | 2016-09-07 | 深圳普门科技有限公司 | Erythrocyte-osmotic-fragility measuring method based on scattering turbidimetric technology |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0252127B1 (en) * | 1985-12-23 | 1990-10-03 | Beckman Instruments, Inc. | Apparatus and method for nephelometric measurement of reactions |
| CN1734254A (en) * | 2004-08-10 | 2006-02-15 | 南京英诺华科技有限公司 | Automatic foodstuff composition analyser |
| WO2008151072A1 (en) * | 2007-06-01 | 2008-12-11 | The Regents Of The University Of California | Multigene prognostic assay for lung cancer |
| CN201876418U (en) * | 2010-11-05 | 2011-06-22 | 深圳清华大学研究院 | Immune turbidity measuring device |
| CN102147405A (en) * | 2011-01-12 | 2011-08-10 | 深圳市亚辉龙生物科技有限公司 | Fully automatic biochemical immune analyzer |
| CN102262164A (en) * | 2011-07-20 | 2011-11-30 | 长春迪瑞医疗科技股份有限公司 | Liquid-way system of fully-automatic fecal occult blood analyzer |
| CN102288581A (en) * | 2011-07-29 | 2011-12-21 | 南京诺尔曼生物技术有限公司 | Specific protein measuring method and device |
| CN203432975U (en) * | 2013-07-05 | 2014-02-12 | 深圳普门科技有限公司 | Full-automatic in-vitro detection equipment for measuring specific proteins by turbidimetry |
-
2013
- 2013-07-05 CN CN2013102818566A patent/CN103344642A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0252127B1 (en) * | 1985-12-23 | 1990-10-03 | Beckman Instruments, Inc. | Apparatus and method for nephelometric measurement of reactions |
| CN1734254A (en) * | 2004-08-10 | 2006-02-15 | 南京英诺华科技有限公司 | Automatic foodstuff composition analyser |
| WO2008151072A1 (en) * | 2007-06-01 | 2008-12-11 | The Regents Of The University Of California | Multigene prognostic assay for lung cancer |
| CN201876418U (en) * | 2010-11-05 | 2011-06-22 | 深圳清华大学研究院 | Immune turbidity measuring device |
| CN102147405A (en) * | 2011-01-12 | 2011-08-10 | 深圳市亚辉龙生物科技有限公司 | Fully automatic biochemical immune analyzer |
| CN102262164A (en) * | 2011-07-20 | 2011-11-30 | 长春迪瑞医疗科技股份有限公司 | Liquid-way system of fully-automatic fecal occult blood analyzer |
| CN102288581A (en) * | 2011-07-29 | 2011-12-21 | 南京诺尔曼生物技术有限公司 | Specific protein measuring method and device |
| CN203432975U (en) * | 2013-07-05 | 2014-02-12 | 深圳普门科技有限公司 | Full-automatic in-vitro detection equipment for measuring specific proteins by turbidimetry |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901204A (en) * | 2014-04-11 | 2014-07-02 | 深圳市儿童医院 | Immunoturbidimetry kit for detecting streptococcus agalactiae and detection method of immunoturbidimetry kit |
| CN103901204B (en) * | 2014-04-11 | 2016-01-27 | 深圳市儿童医院 | A kind of immunoturbidimetry kit and detection method thereof detecting Streptococcusagalactiae |
| CN104535776A (en) * | 2014-12-05 | 2015-04-22 | 深圳普门科技有限公司 | Immune scatter turbidimetry based full-automatic detection device and method thereof |
| CN105928907A (en) * | 2016-05-17 | 2016-09-07 | 深圳普门科技有限公司 | Erythrocyte-osmotic-fragility measuring method based on scattering turbidimetric technology |
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