CN103027004B - Long-distance transportation method of sepia esculenta adults - Google Patents
Long-distance transportation method of sepia esculenta adults Download PDFInfo
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- CN103027004B CN103027004B CN201310007932.4A CN201310007932A CN103027004B CN 103027004 B CN103027004 B CN 103027004B CN 201310007932 A CN201310007932 A CN 201310007932A CN 103027004 B CN103027004 B CN 103027004B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a long-distance transportation method of sepia esculenta adults, and belongs to the field of aquatic product transportation. The long-distance transportation method comprises the steps of two-step oxygenation and two-step anesthetization, wherein the transportation time is not more than 20h; the two-step oxygenation comprises the following steps: oxygenating water for transporting sepia esculenta in a water reservoir with a nano-airstone until saturation, and oxygenating a transportation bag after the sepia esculenta adults are placed into the transportation bag; and the two-step anesthetization comprises the following steps: adding 30-60mg/L of MS-222 anesthetic to a sepia esculenta culture pond without disturbing the sepia esculenta adults, and instantly weighing and packing for transportation when the sepia esculenta adults are anesthetized until the sepia esculenta adults stop swinging the lateral fins and lose the sense of balance; and adding 5-8mg/L of MS-222 anesthetic to the water for transportation, so that the sepia esculenta adults maintain a two-stage anesthetic transportation state, namely, the sepia esculenta adults slightly lose the touch sense, fold the wrists, swing the fins naturally, and changes the body color slightly. With adoption of the long-distance transportation method of the sepia esculenta adults, the survival rate of the sepia esculenta adults during long-distance transportation is significantly increased, and the technical problem about the long-distance transportation of the sepia esculenta adults is solved.
Description
Technical field
The invention belongs to aquatic product transportation field, relate to particularly a kind of golden cuttlefish adult long-distance transport method.
Background technology
Under field conditions (factors), when facing a danger, ink-jet is cephalopodous a kind of escape behavior, near the seawater of the prepared Chinese ink of ejection can making becomes black, thereby hidden own, and by the current escape of quick ejection, in addition prepared Chinese ink also contains toxin, and harmful animal is also had to certain attacking role.In breeding process, find equally, during fight between parent purchase, fall pond operation and individuality siphonopods also as easy as rolling off a log generation ink-jet behavior, especially long-distance transport front pack, weigh and transportation in ink-jet phenomenon even more serious.The ink-jet behavior of siphonopods in transportation not only damages physique, also easily ruins water quality, causes transportation survival rate lower, limited to a certain extent the development of its industry.
Aspect siphonopods research, abroad focus mostly in basic research such as Resources of Cephalopods investigation and biological propertyes, Chinese aspect siphonopods aquaculture in rank first.At present, the principal item of domestic siphonopods exploitation cultivation is golden cuttlefish, Sepiella maindroni, octopus (true octopus, short octopus and long octopus) etc.In these kinds, octopus class is due to without inner casing, health softness, and ink ejection amount is little, adopts low-temperature transport survival rate very high, does not have transportation technology bottleneck.And golden cuttlefish and Sepiella maindroni have hard inner casing, not low temperature resistant, and irritant reaction sensitivity to external world, as easy as rolling off a log ink-jet and collision in transportation, finally because water quality is ruined or somatic damage dead.Both compare, and golden cuttlefish individuality is larger, is 4～5 times of Sepiella maindroni, therefore ink ejection amount is larger, and golden cuttlefish has tailpin, after collision, health tail muscles also more easily damages and causes tailpin and inner casing to expose, and therefore golden cuttlefish long-distance transport difficulty is larger.At present golden cuttlefish is mainly take short-distance transport as main, and method is mainly used for reference fish plastic sack oxygenation transportation, but the method golden cuttlefish the ink-jet rate of 4～5 hours up to more than 60%, transportation survival rate is extremely low, is only 40%.Therefore golden cuttlefish long-distance transport technology has limited the popularization cultivation of golden cuttlefish to a certain extent, is the bottleneck of its Industry Promotion.
Anaesthetic can make aquatic livestock peace and quiet, and then reduces the stress reaction while adopting the operation such as ovum, semen collection, blood sampling, makes to operate smooth.In addition,, in transportation, anaesthetic can reduce metabolism, the injury of minimizing to animal bodies of aquatic livestock, and then improves transportation survival rate.Therefore, anaesthetic obtains certain application in aquaculture.Fish is more with anaesthetic at present, comprise nearly 30 kinds of MS-222, sulfuric acid quinaldine, eugenol, 2-Phenoxyethanol etc., wherein MS-222(chemistry alkylsulfonate coordination benzocaine by name) there is aquatic livestock and enter the advantages such as fast and recovery time of fiber crops is short, to be that our times various countries are widely used in the safest in aquatic livestock, the most reliably, the most effective anaesthetic, and through FDA(food and drug administration) approval, for aquatic livestock anesthesia transportation.
Aspect siphonopods transportation, Japanology squid live body long-distance transport method, mainly contain three kinds: 1) remove ink sac; 2) use ethene and add the seawater of special oxide, and water temperature is controlled to 5 ℃ of left and right; 3) with the seawater " hypothermic anesthesia " of-2 ℃.Although adopt these methods to transport for long-distance, the squid survival after long-distance transport is shorter, is only 12h～1 week, and therefore these methods are unwell to the transportation of siphonopods parent.At home, the principle of the employing anesthesia such as Wu Changwen has been invented a kind of siphonopods parent live transport pre-treating method and special agent (application number: 201010607873.0), open up Liao Yitiaoxin road for siphonopods transports.But because siphonopods kind is more, there is interspecific difference in different cultivars, the selection of anesthesia kind, concentration, narcosis etc. are also not the same, and the zeolite granular of selecting in this invention in addition only absorbs ammonia nitrogen and prepared Chinese ink, but is dissolved into the toxin in water after cannot removing ink-jet.
That golden cuttlefish has is individual large, ink ejection amount is large, oxygen demand is large, stress reaction is fierce and inner casing tool tailpin very easily causes the features such as somatic damage, therefore control not ink-jet in golden cuttlefish operation and transportation, improve transportation water-soluble oxygen, keeping transportation peace and quiet is the keys that improve its long-distance transport survival rate.Have no at present the report of golden cuttlefish adult long-distance transport method both at home and abroad.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of golden cuttlefish adult long-distance transport method, selects best narcosis that golden cuttlefish is operated and transported, to reduce the generation of ink-jet behavior; Improve transportation water-soluble oxygen; Keep transportation quiet, to reduce stress reaction, and then solve lower this technical bottleneck of golden cuttlefish long-distance transport survival rate.
The present invention completes by following operating technology:
A kind of golden cuttlefish adult long-distance transport method, comprises the step that two step oxygenations and two steps are anaesthetized, and haulage time is no more than 20h;
Two described step oxygenations: to before the water extraction for transporting golden cuttlefish at tank with the oxygenation of nanometer gas stone to saturated, after golden cuttlefish is put into shipping bags again in shipping bags oxygenation;
Two described step anesthesia: in the situation that not alarming golden cuttlefish, in golden cuttlefish culturing pool, add the MS-222 anaesthetic of 30～60mg/L, when golden cuttlefish, anaesthetize to 4 phases of narcosis, wrist scatters, lateral fin stops swinging, and when the sense of equilibrium loses, weighs immediately and packs transportation; In transportation water, add the MS-222 anaesthetic of 5～8mg/L, so that golden cuttlefish maintains 2 phases of narcosis, i.e. sense of touch is slightly lost, and wrist closes up, and fin swings nature, and body colour slightly changes.
Further, in golden cuttlefish culturing pool, add the MS-222 anaesthetic of 30～40mg/L.
Further, described shipping bags is double-deck black polyethylene plastic bag.
Further, the water temperature of the transportation water in described shipping bags is 16～18 ℃, and the oxygen being filled with in shipping bags is 2:1～4:1 with the volume ratio of transportation water.
Further, before golden cuttlefish culturing pool is added to anaesthetic, first the temperature of breeding water is dropped to 16 ~ 18 ℃.
Further, described cool-down method is for adding ice cube or ice chest cooling, and through 2～3 cooling processing step by step, each temperature difference must not exceed 3～5 ℃.
The beneficial effect that the present invention is compared with the prior art:
1) clear and definite first golden cuttlefish anesthesia level by stages, has been determined suitable anesthetic concentration and mode of operation, and the narcosis of narcotic concentration and golden cuttlefish during transportation.
2) the present invention adopts the step of two step anesthesia, to carrying out high concentration anesthesia procedure before golden cuttlefish transportation, low concentration anesthesia in transportation, not only controlled the generation of ink-jet behavior when pond, weigh and packing, avoid physique damage, also reduced transportation consumption rate, controlled the ink-jet behavior in transportation, ensured transportation water quality, and then significantly improved golden cuttlefish long-distance transport survival rate, solved golden cuttlefish adult long-distance transport technical barrier.
3) the Promethean two step oxygenation steps that adopted of the present invention, in bulk container, adopt nanometer gas stone that transportation water is filled to pure oxygen to saturated, then join and in plastic sack, carry out again oxygenation sealing, 7mg/L when dissolved oxygen in water is directly sealed plastic sack oxygenation by prior art is increased to 15mg/L, has significantly improved the dissolved oxygen amount of transportation water.
4) the double-deck white polyethylene plastic sack that the present invention generally adopts aquatic livestock plastic sack oxygenation transportation changes double-deck black polyethylene plastic bag into, played shaded effect in transportation, thereby make golden cuttlefish calmness, to reduce collision behavior, and then reduced shipping damage.
5) adopt the present invention to transport golden cuttlefish adult, after transportation, cultivate the time-to-live longer, until natural death does not affect parent mating breeding.
Accompanying drawing explanation
The MS-222 anaesthetic of Fig. 1 variable concentrations is to the final narcosis of golden cuttlefish
Embodiment
Below by embodiment, describe technical method of the present invention in detail:
Embodiment 1
(1) according to golden cuttlefish anesthesia and recovery behavior by stages, then determine suitable golden cuttlefish anesthesia procedure concentration and anesthesia transportation concentration
Experimental scheme 2,5,8,15,20,30,40,60 and 80mg/L9 concentration gradient, 5 of each concentration test golden cuttlefish adults, measure the final anesthesia level that in 24h, golden cuttlefish reaches, and observe the behavioural characteristic under final narcosis, then proceed in clean sea water and recover, observe the behavioural characteristic in recovery process, and then anesthesia level and recovery process are carried out by stages.
Result of the test: 1) according to the behavioural characteristic under final narcosis, anesthesia level is divided into six periods, in Table 1.
Table 1 anesthesia level by stages and behavioural characteristic
| Anesthesia level by stages | Behavioural characteristic |
| 0 phase | Wrist closes up, and fin swings normal, the nature that moves about, and body colour is normal |
| 1 phase | Sense of touch is slightly lost, and wrist closes up, and fin swings normal, the nature that moves about, and body colour is normal |
| 2 phases | Sense of touch is slightly lost, and wrist closes up, and fin swings nature, and body colour slightly changes |
| 3 phases | Lateral fin swings to be accelerated, and moves about rapid, and wrist closes up, and body colour reddens |
| 4 phases | Wrist scatters, and lateral fin stops swinging, and the sense of equilibrium loses |
| 5 phases | There is ink-jet behavior |
| 6 phases | Sign of life stops, individual death |
2) according to recovery feature, recovery process is divided into four periods, in Table 2.
Table 2 recovery process by stages and behavioural characteristic
| Recovery process by stages | Behavioural characteristic |
| 1 phase | Swim in the water surface, lateral fin is motionless, and wrist opens |
| 2 phases | Swim in the water surface, lateral fin starts to swing |
| 3 phases | Swim in the water surface, wrist closes up gradually |
| 4 phases | Beginning in lower water layer move about |
According to golden cuttlefish anesthesia and recovery behavior state feature, through experimental verification repeatedly, determine that the suitable operation narcosis of MS-222 anesthesia golden cuttlefish was 4 phases, wrist scatters, and lateral fin stops swinging, and the sense of equilibrium loses; As shown in Figure 1, in breeding water body, anaesthetic concentration is 30～60mg/L, and golden cuttlefish all can reach 4 phases of narcosis, and the time used is 3～5min; Suitable anesthesia transportation concentration 5～8mg/L, narcosis was 2 phases, i.e. and sense of touch is slightly lost, and wrist closes up, and fin swings nature, and body colour slightly changes.
Embodiment 2:
In June, 2012, we are transported to prosperous Yongfeng, Tianjin aquaculture Co., Ltd 250 golden cuttlefish adults by Golden Beach, Qingdao aquatic products development corporation, Ltd..Transportation water is cooled to 18 ℃ with ice cube by 24 ℃, adds that with nanometer gas stone, to fill pure oxygen after the MS-222 of 7mg/L extremely saturated; To in the golden cuttlefish culturing pool of stopping eating 1 day, add ice cube or ice chest cooling, through 2 cooling processing step by step, each temperature difference must not exceed 3 ℃, cools the temperature to 18 ℃; Then adding final concentration is the MS-222 of 30mg/L, and after 5min, golden cuttlefish enters 4 phases of narcosis, and wrist scatters, lateral fin stops swinging, the sense of equilibrium loses, and immediately golden cuttlefish is dragged for gently to washbowl, after weighing, slowly with hand, pulls out and puts into the black plastic bag that 10L transportation water is housed, put 1 of golden cuttlefish adult for every bag, squeeze out the air in bag, be filled with about 30L oxygen, rubber band is put into bubble chamber after tightening sack, every case is put 2 bags, then adds 2 freezing mineral water bottles.After bubble chamber being obturaged with adhesive tape, put into thermal car and transport, in transportation, compartment temperature keeps 17～19 ℃.After arriving Tianjin, plastic sack was put into the seawater transition of 20 ℃ preparing after 15 minutes, then open bag golden cuttlefish is poured in fresh seawater.From the Qingdao land transportation transportation result of 11 hours to Hangu, Tianjin, show, golden cuttlefish transportation ink-jet rate is only 2%, and survival rate is up to 96%, thereby efficiently solves golden cuttlefish long-distance transport technical barrier.
Embodiment 3:
In July, 2012, we are transported to Xin Hai prosperous nursery in Xiapu, Fujian 150 golden cuttlefish adults by Huanghai Sea aquatic products research institute Huang Island golden cuttlefish Experimental Base.Transport ice block cooling to 16 ℃ for water, add the MS-222 of 8mg/L, then with nanometer gas stone, fill pure oxygen to saturated; To in the golden cuttlefish culturing pool of stopping eating 1 day, add ice cube or ice chest cooling, through 3 cooling processing step by step, each temperature difference is no more than 5 ℃, cools the temperature to 16 ℃; Then adding final concentration is the MS-222 of 60mg/L, and after 3min, golden cuttlefish enters 4 phases of narcosis, and wrist scatters, lateral fin stops swinging, the sense of equilibrium loses, and immediately golden cuttlefish is dragged for gently to washbowl, after weighing, slowly with hand, pulls out and puts into the black plastic bag that 10L transportation water is housed, put 1 of golden cuttlefish adult for every bag, squeeze out the air in bag, be filled with about 30L oxygen, rubber band is put into bubble chamber after tightening sack, every case is put 2 bags, then adds 2 freezing mineral water bottles.After bubble chamber being obturaged with adhesive tape, put into thermal car and transport, in transportation, compartment temperature keeps 16 ℃ of left and right.After arriving Fujian, plastic sack was put into the seawater transition of 20 ℃ preparing after 15 minutes, then open bag golden cuttlefish is poured in fresh seawater.From the Qingdao land transportation transportation result of 15 hours to Xiapu, Fujian, show, transportation ink-jet rate is only 3%, and survival rate is up to 94%, and this golden cuttlefish long-distance transport technology is further verified.
Claims (6)
1. a golden cuttlefish adult long-distance transport method, is characterized in that it comprises the step that two step oxygenations and two steps are anaesthetized, and haulage time is no more than 20h;
Two described step oxygenations: to before the water extraction for transporting golden cuttlefish at tank with the oxygenation of nanometer gas stone to saturated, secondly after golden cuttlefish is put into shipping bags again in shipping bags oxygenation;
Two described step anesthesia: in the situation that not alarming golden cuttlefish, in golden cuttlefish culturing pool, add the MS-222 anaesthetic of 30～60mg/L, when golden cuttlefish, anaesthetize to 4 phases of narcosis, wrist scatters, lateral fin stops swinging, and when the sense of equilibrium loses, weighs immediately and packs; In transportation water, add the MS-222 anaesthetic of 5～8mg/L, so that golden cuttlefish maintains 2 phases of narcosis, i.e. sense of touch is slightly lost, and wrist closes up, and fin swings nature, and body colour slightly changes.
2. a kind of golden cuttlefish adult long-distance transport method according to claim 1, is characterized in that adding the MS-222 anaesthetic of 30～40mg/L in golden cuttlefish culturing pool.
3. a kind of golden cuttlefish adult long-distance transport method according to claim 1, is characterized in that described shipping bags is double-deck black polyethylene plastic bag.
4. a kind of golden cuttlefish adult long-distance transport method according to claim 1, is characterized in that the water temperature of the transportation water in described shipping bags is 16～18 ℃, and the oxygen being filled with in shipping bags is 2:1～4:1 with the volume ratio of transportation water.
5. a kind of golden cuttlefish adult long-distance transport method according to claim 1, is characterized in that, before golden cuttlefish culturing pool is added to anaesthetic, first the temperature of breeding water being dropped to 16～18 ℃.
6. a kind of golden cuttlefish adult long-distance transport method according to claim 5, is characterized in that described cool-down method is for adding ice cube or ice chest cooling, through 2～3 cooling processing step by step, 3～5 ℃ of each temperature difference.
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| CN103798171B (en) * | 2014-02-22 | 2015-07-15 | 上海海洋大学 | No-water transportation method of aquatic product |
| CN103843711B (en) * | 2014-04-03 | 2015-12-30 | 山东东方海洋科技股份有限公司 | The transportation resources of a kind of salmon trout |
| CN104145860B (en) * | 2014-08-01 | 2016-04-06 | 中国水产科学研究院长江水产研究所 | A kind of long Qi Wen Minnow parent population transportation resources |
| CN105325322A (en) * | 2014-08-12 | 2016-02-17 | 中国科学院水生生物研究所 | Method improving Mongolia culter fry transportation survival rate |
| CN104521810B (en) * | 2014-12-04 | 2018-05-01 | 中国科学院南海海洋研究所 | A kind of hippocampus anesthesia and transportation resources |
| CN104996339A (en) * | 2015-07-24 | 2015-10-28 | 浙江师范大学 | Pre-treatment mixed reagent for transportation of symphysodon aequfasciatuslive bodies |
| CN105052810A (en) * | 2015-09-09 | 2015-11-18 | 岭南师范学院 | Kisslip cuttlefish fry long-distance alive-keeping transport method |
| CN105210943B (en) * | 2015-10-08 | 2018-07-24 | 山东省海洋生物研究院 | A kind of spot trout live fish hypothermic anesthesia transportation resources |
| CN111387091A (en) * | 2020-05-25 | 2020-07-10 | 中国水产科学研究院黄海水产研究所 | Long-distance transportation method of adult Sepiella maindroni |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH02119730A (en) * | 1988-10-28 | 1990-05-07 | Shuzo Kamata | Method for transporting live cuttlefish and transporting container |
| CN101703026B (en) * | 2009-11-17 | 2012-07-04 | 浙江海洋学院 | Squid living body transportation method and special ink control chute |
| CN201640203U (en) * | 2009-11-17 | 2010-11-24 | 浙江海洋学院 | Squid pre-shipment treatment plant |
| CN101731161B (en) * | 2009-12-20 | 2012-06-13 | 浙江海洋学院 | Subcutaneous fluorescent labeling method for Sepiella maindroni and special reagent thereof |
| CN102037951A (en) * | 2010-12-15 | 2011-05-04 | 浙江海洋学院 | PH stabilizer for transporting cuttlefish live bodies |
| CN102113480A (en) * | 2010-12-15 | 2011-07-06 | 浙江海洋学院 | Pretreatment method and special reagent for living body transportation of parents of cephalopods |
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