CN102998097A - Attenuated total reflection optical measurement platform - Google Patents
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Abstract
本发明公开了一种衰减全反射光学测量平台,涉及光学测量技术,包括底座、转动臂、固定臂、测试部件等;测试部件为光波导测试装置或光波导生化传感器测试装置或棱镜全反射测试装置。固定臂与固定圆盘成一整体,转动臂与固定圆盘同轴转动连接,转动臂通过紧固螺钉锁定;固定臂与固定圆盘置于底座上方,与底座固定连接,转动臂位于底座与固定圆盘之间;测角转盘置于固定圆盘上方,与固定圆盘同轴固定连接;测试部件通过自身支杆固定在测角转盘上,光源和探测器分别固定到各自支架上,光源支架和探测器支架再通过支杆分别固定到固定臂和转动臂上,光源光穿过测试部件后被探测器接收。本发明装置入射角准确可调,可灵活接收出射光,重复性和通用性好。
The invention discloses an attenuated total reflection optical measurement platform, which relates to optical measurement technology, and includes a base, a rotating arm, a fixed arm, a test component, etc.; the test component is an optical waveguide test device or an optical waveguide biochemical sensor test device or a prism total reflection test device. The fixed arm is integrated with the fixed disc, the rotating arm is coaxially connected with the fixed disc, and the rotating arm is locked by fastening screws; the fixed arm and the fixed disc are placed above the base and fixedly connected with the base, and the rotating arm is located between the base and the fixed disc. Between the discs; the angle measuring turntable is placed above the fixed disc, and is coaxially fixedly connected with the fixed disc; the test component is fixed on the angle measuring turntable through its own pole, and the light source and detector are respectively fixed on their respective brackets, and the light source bracket and the detector bracket are respectively fixed on the fixed arm and the rotating arm through the support rod, and the light from the light source passes through the test component and is received by the detector. The device of the invention has an accurate and adjustable incident angle, can flexibly receive outgoing light, and has good repeatability and versatility.
Description
技术领域 technical field
本发明涉及光学测量技术领域,是一种基于耦合棱镜的衰减全反射光学测量平台,用于测试光波导导波特性、光波导传感器特性、表面等离子体共振传感器特性、薄膜样品厚度和折射率、溶液浓度等。The invention relates to the field of optical measurement technology, and is an attenuated total reflection optical measurement platform based on a coupling prism, which is used to test the characteristics of optical waveguide guide waves, optical waveguide sensor characteristics, surface plasmon resonance sensor characteristics, film sample thickness and refractive index , solution concentration, etc.
背景技术 Background technique
衰减全反射技术是一种重要的光学技术,在光耦合、光传感、光谱分析、薄膜样品测试等许多方面具有广泛应用。光在棱镜底面发生全反射时会产生消逝场,该消逝场可用于激发表面等离子体激元(SPP),光波导导模,荧光、拉曼信号、SHG信号,也可被分子吸收,使得反射光能量被衰减。棱镜全反射器在光波导器件、表面等离子体共振(SPR)传感器中常被作为光耦合器使用,而棱镜全反射器本身也是一种重要的消逝波敏感器件,常被用于薄膜试样分析、液体浓度探测以及吸附分子的光谱测试等方面。现有的棱镜衰减全反射装置比较专一,通用性差,不能兼顾上述各种用途。尤其是基于棱镜耦合的光波导传感器在使用时常采用分离器件进行组装和光路调试,不便于操作,系统稳定性不高,测试重复性差。目前还缺少可用于测试光波导传感器的小型化衰减全反射测量系统,更缺少既可用于光波导测试、又可用于SPR测试、还可进行棱镜全反射测量的通用性测试平台。Attenuated total reflection technology is an important optical technology, which has a wide range of applications in many aspects such as optical coupling, optical sensing, spectral analysis, and thin film sample testing. When the light is totally reflected at the bottom of the prism, an evanescent field will be generated. This evanescent field can be used to excite surface plasmon polaritons (SPP), optical waveguide modes, fluorescence, Raman signals, and SHG signals. It can also be absorbed by molecules, making the reflection Light energy is attenuated. The prism total reflector is often used as an optical coupler in optical waveguide devices and surface plasmon resonance (SPR) sensors, and the prism total reflector itself is also an important evanescent wave sensitive device, which is often used for thin film sample analysis, Liquid concentration detection and spectral testing of adsorbed molecules. Existing prism attenuated total reflection devices are relatively specific, have poor versatility, and cannot take into account the above-mentioned various purposes. In particular, optical waveguide sensors based on prism coupling often use separate devices for assembly and optical path debugging, which is not easy to operate, the system stability is not high, and the test repeatability is poor. At present, there is still a lack of a miniaturized attenuated total reflection measurement system that can be used to test optical waveguide sensors, and there is still a lack of a universal test platform that can be used for optical waveguide testing, SPR testing, and prism total reflection measurement.
发明内容 Contents of the invention
本发明的目的是提供一种小型化衰减全反射光学测量平台,其结构简单实用,操作方便,用途广泛,解决了现有相关装置的器件分离、操作复杂、精度不高、稳定性和重复性差、缺少通用性的问题。The purpose of the present invention is to provide a miniaturized attenuated total reflection optical measurement platform, which has simple and practical structure, convenient operation and wide application, and solves the problems of device separation, complicated operation, low precision, poor stability and repeatability of existing related devices , Lack of versatility.
为实现上述目的,本发明的技术解决方案是:For realizing the above object, technical solution of the present invention is:
一种衰减全反射光学测量平台,包括底座、转动臂、固定臂、固定圆盘、测角转盘、光源、光源支架、探测器、探测器支架、测试部件及支杆;其中,An attenuated total reflection optical measurement platform, including a base, a rotating arm, a fixed arm, a fixed disc, an angle measuring turntable, a light source, a light source bracket, a detector, a detector bracket, a test component and a support rod; wherein,
在固定圆盘侧面径向凸设有固定臂,固定圆盘与固定臂成一整体,固定圆盘中心通孔套设有轴,轴上端与固定圆盘固接,轴下端支于底座中心通孔中,固定臂与固定圆盘水平置于底座上方,由至少三组螺钉和配套垫圈与底座固定连接,固定圆盘侧面设有凹槽;固定臂外端上表面有一凹槽;A fixed arm is radially protruded from the side of the fixed disc. The fixed disc and the fixed arm are integrated. The center through hole of the fixed disc is provided with a shaft. Among them, the fixed arm and the fixed disc are placed horizontally above the base, and are fixedly connected to the base by at least three sets of screws and supporting washers, the side of the fixed disc is provided with a groove; the upper surface of the outer end of the fixed arm has a groove;
转动臂内端通孔套于轴上,与固定圆盘同轴转动连接,位于底座与固定圆盘之间,转动臂外端上表面有一凹槽;转动臂内外两端之间向上凸设螺钉支架,螺钉支架中心水平螺设紧固螺钉,紧固螺钉内端头与固定圆盘侧凹槽相适配,通过紧固螺钉挤压凹槽即锁定转动臂;The through hole at the inner end of the rotating arm is sleeved on the shaft, and is coaxially connected with the fixed disc. It is located between the base and the fixed disc. There is a groove on the upper surface of the outer end of the rotating arm; screws are protruding upwards between the inner and outer ends of the rotating arm. Bracket, the center of the screw bracket is horizontally screwed with a fastening screw, and the inner end of the fastening screw is adapted to the side groove of the fixed disc, and the rotating arm is locked by squeezing the groove with the fastening screw;
测角转盘水平置于固定圆盘上方,测角转盘底盘与固定圆盘同轴固定连接,测角转盘侧面径向凸设有角度微调螺杆,测角转盘上转动盘中心有通孔;The angle measuring turntable is placed horizontally above the fixed disc, the chassis of the angle measuring turntable is coaxially fixedly connected with the fixed disc, the side of the angle measuring turntable is radially protruded with an angle fine-tuning screw, and the center of the turntable on the angle measuring turntable has a through hole;
固定臂外端凹槽中正交固接第一支杆下端,第一支杆上端可旋转的固接光源支架,光源固定在光源支架上;测角转盘上转动盘中心通孔内侧壁中正交固接第二支杆下端周圆,第二支杆上端固接测试部件,第二支杆与轴共一中心轴线;转动臂外端凹槽中正交固接第三支杆下端,第三支杆上端可旋转的固接探测器支架,探测器固定在探测器支架上;光源、测试部件及探测器的中心到底座上表面的距离相同,光源、测试部件、探测器处于同一水平面,光源光被耦合穿过测试部件后,被探测器接收。The lower end of the first support rod is fixed orthogonally in the groove at the outer end of the fixed arm, and the upper end of the first support rod is rotatably fixed to the light source bracket, and the light source is fixed on the light source support; The circumference of the lower end of the second support rod is cross-fixed, and the upper end of the second support rod is fixed to the test part. The second support rod and the shaft share a central axis; The upper ends of the three rods are rotatably fixed to the detector bracket, and the detector is fixed on the detector bracket; the distance from the center of the light source, test component and detector to the upper surface of the base is the same, and the light source, test component and detector are on the same horizontal plane. Light from the source is coupled through the test part and received by the detector.
所述的衰减全反射光学测量平台,其所述测试部件,为光波导测试装置(发明专利,申请号:200910080063.1,申请日:2009年3月18日)或光波导生化传感器测试装置(发明专利,申请号:200910093766.8,申请日:2009年9月28日)或棱镜全反射测试装置其中之一。The attenuated total reflection optical measurement platform, the test components, is an optical waveguide testing device (invention patent, application number: 200910080063.1, application date: March 18, 2009) or an optical waveguide biochemical sensor testing device (invention patent , application number: 200910093766.8, application date: September 28, 2009) or one of the prism total reflection test devices.
所述的衰减全反射光学测量平台,其所述棱镜全反射测试装置,包括底盘、耦合棱镜、光传感芯片、样品槽、样品槽紧固件、第二支杆,其中,耦合棱镜为等腰直角棱镜,耦合棱镜的一侧面固接于底盘上表面,两直角边侧面分别对向两侧的光源、探测器,耦合棱镜的底面与光传感芯片背面紧贴连,光传感芯片正面与样品槽开口紧贴连,样品槽底外侧与样品槽紧固件紧贴连;样品槽紧固件固接于底盘上表面;第二支杆上端正交固接于底盘下表面,第二支杆的轴线与耦合棱镜底面一直角边垂直相交;The attenuated total reflection optical measurement platform, the prism total reflection test device includes a chassis, a coupling prism, an optical sensor chip, a sample slot, a sample slot fastener, and a second pole, wherein the coupling prism is, etc. Waist right-angle prism, one side of the coupling prism is fixed on the upper surface of the chassis, and the two right-angle sides face the light sources and detectors on both sides respectively. It is closely connected with the opening of the sample tank, and the outer side of the bottom of the sample tank is closely connected with the fasteners of the sample tank; the fasteners of the sample tank are fixed on the upper surface of the chassis; the upper end of the second pole is orthogonally fixed on the lower surface of the chassis, and the second The axis of the strut is vertically intersected with the right-angled side of the bottom surface of the coupling prism;
使用时,将少许耦合液滴在光传感芯片背面,然后将光传感芯片背面紧贴耦合棱镜底面,再将样品槽紧贴光传感芯片正面,通过调节样品槽紧固件,使样品槽和光传感芯片紧夹在耦合棱镜和样品槽紧固件之间,并使样品槽与光传感芯片接触处不漏水。When in use, put a little coupling liquid on the back of the light sensor chip, then put the back of the light sensor chip close to the bottom of the coupling prism, and then put the sample groove close to the front of the light sensor chip, and adjust the fasteners of the sample groove to make the sample The groove and the light sensing chip are tightly clamped between the coupling prism and the sample groove fastener, and the contact between the sample groove and the light sensing chip is watertight.
所述的衰减全反射光学测量平台,其所述耦合棱镜,为玻璃或透光晶体制成;光传感芯片,为表面等离子体共振芯片或光波导芯片其中之一;样品槽,由硅橡胶或聚四氟乙烯材料制成;样品槽紧固件包括压块、横梁和紧固螺钉,横梁下侧固接于底盘上表面,紧固螺钉水平螺设于横梁中部,紧固螺钉内端有压块,外端为旋把;调节旋把,使压块将样品槽和光传感芯片紧贴连,并使样品槽与光传感芯片接触处不漏水。In the attenuated total reflection optical measurement platform, the coupling prism is made of glass or light-transmitting crystal; the optical sensor chip is one of a surface plasmon resonance chip or an optical waveguide chip; the sample slot is made of silicon rubber or polytetrafluoroethylene; the fasteners of the sample tank include a pressure block, a beam and a fastening screw. The pressing block has a rotary handle at the outer end; adjust the rotary handle so that the pressing block closely connects the sample tank and the light sensor chip, and makes the contact between the sample tank and the light sensor chip leak-free.
所述的衰减全反射光学测量平台,其所述光源,为小型线偏振激光器或宽带线偏振平行光发射器其中之一。In the attenuated total reflection optical measurement platform, the light source is one of a small linearly polarized laser or a broadband linearly polarized parallel light emitter.
所述的衰减全反射光学测量平台,其所述探测器,为光电池、光敏二极管、光电倍增管或光纤光谱仪其中之一;当光源为小型线偏振激光器时,探测器用光电池、光敏二极管或光电倍增管中的一种;当光源为宽带线偏振平行光发射器时,探测器用光纤光谱仪。In the attenuated total reflection optical measurement platform, the detector is one of photocell, photodiode, photomultiplier tube or fiber optic spectrometer; when the light source is a small linearly polarized laser, the detector uses a photocell, photodiode or photomultiplier One of the tubes; when the light source is a broadband linearly polarized parallel light emitter, the detector uses a fiber optic spectrometer.
所述的衰减全反射光学测量平台,其所述宽带线偏振平行光发射器,包括一光纤宽带冷光源,一光纤准直器、光源支架、一线性偏振器、及一光纤;光纤一端与光纤宽带冷光源固定连接,另一端与光纤准直器后端固定连接,线性偏振器置于光纤准直器前端,光纤准直器、线性偏振器分别固定在光源支架上,并使光纤准直器射出的平行光垂直穿过线性偏振器;光源支架固接在第一支杆上端,第一支杆下端垂直固定在固定臂上,光源支架上设有微调旋钮,使光纤准直器射出的平行光束轴线与测角转盘轴线垂直相交。Described attenuated total reflection optical measurement platform, its described broadband linearly polarized parallel light emitter, comprises a fiber optic broadband cold light source, a fiber optic collimator, light source support, a linear polarizer, and an optical fiber; The broadband cold light source is fixedly connected, and the other end is fixedly connected to the rear end of the fiber collimator. The linear polarizer is placed at the front end of the fiber collimator. The emitted parallel light passes through the linear polarizer vertically; the light source bracket is fixed on the upper end of the first support rod, and the lower end of the first support rod is vertically fixed on the fixed arm. The axis of the beam is perpendicular to the axis of the goniometer turntable.
所述的衰减全反射光学测量平台,其当选用光纤光谱仪作为探测器时,需要如下附件:第二光纤、第二光纤准直器、探测器支架,第二光纤一端与光纤光谱仪固定连接,另一端与第二光纤准直器固定连接,第二光纤准直器固定在探测器支架上,探测器支架固接在第三支杆上端,第三支杆下端垂直固定在转动臂上;从测试部件输出的光束被第二光纤准直器接收并聚焦到第二光纤端面,再由第二光纤传输到光纤光谱仪;光纤光谱仪与第一光纤准直器之间以第三光纤相连接。The attenuated total reflection optical measurement platform, when the optical fiber spectrometer is selected as the detector, the following accessories are required: a second optical fiber, a second optical fiber collimator, a detector bracket, one end of the second optical fiber is fixedly connected to the optical fiber spectrometer, and the other One end is fixedly connected with the second fiber collimator, the second fiber collimator is fixed on the detector bracket, the detector bracket is fixed on the upper end of the third rod, and the lower end of the third rod is vertically fixed on the rotating arm; from the test The light beam output by the component is received by the second fiber collimator and focused to the end face of the second fiber, and then transmitted to the fiber optic spectrometer by the second fiber; the fiber spectrometer and the first fiber collimator are connected by a third fiber.
所述的衰减全反射光学测量平台,其所述宽带线偏振平行光发射器与光纤光谱仪配合使用时,宽带线偏振平行光发射器使用的光纤与光纤光谱仪使用的光纤,通过分叉光纤结构互联形成三支四端的两头分叉光纤,使得宽带线偏振平行光发射器中的第一光纤准直器通过分叉光纤同时与光纤冷光源和光纤光谱仪连接,光纤光谱仪又通过分叉光纤同时与作为光纤光谱仪附件的第二光纤准直器连接;In the attenuated total reflection optical measurement platform, when the broadband linearly polarized parallel light emitter is used in conjunction with the fiber optic spectrometer, the optical fiber used by the broadband linearly polarized parallel light emitter and the optical fiber used by the fiber optic spectrometer are interconnected through a bifurcated optical fiber structure Three forked fibers with four ends are formed, so that the first fiber collimator in the broadband linearly polarized parallel light transmitter is connected to the fiber cold light source and the fiber optic spectrometer at the same time through the bifurcated fiber, and the fiber spectrometer is simultaneously connected to the fiber optic spectrometer through the bifurcated fiber. Second fiber collimator connection for fiber optic spectrometer accessories;
当来自第一光纤准直器的平行光束照射到测试部件中的耦合棱镜时,从被照射的耦合棱镜镜面反射的光束,通过调节测角转盘的角度,使其沿原路返回到第一光纤准直器,再由分叉光纤传输到光纤光谱仪进行探测,由此实现测试平台精确的光路准直与零度入射角定位功能。When the parallel beam from the first fiber collimator irradiates the coupling prism in the test component, the beam reflected from the mirror surface of the irradiated coupling prism returns to the first optical fiber along the original path by adjusting the angle of the goniometer turntable The collimator is then transmitted to the fiber optic spectrometer by the bifurcated optical fiber for detection, thereby realizing the precise optical path collimation and zero-degree incident angle positioning functions of the test platform.
所述的衰减全反射光学测量平台,其所述测角转盘,是手动操作的测角转盘,或电机驱动的全自动测角转盘。In the attenuated total reflection optical measurement platform, the angle measuring turntable is a manually operated angle measuring turntable, or a fully automatic angle measuring turntable driven by a motor.
所述的衰减全反射光学测量平台,其所述固定圆盘、转动臂与轴之间分别设有轴承,两轴承叠置;轴上端与固定圆盘固接,是以焊接方式固定连接,轴下端设有不脱出螺钉、垫片。In the attenuated total reflection optical measurement platform, bearings are respectively arranged between the fixed disc, the rotating arm and the shaft, and the two bearings are stacked; the upper end of the shaft is fixedly connected to the fixed disc by welding, and the shaft The lower end is provided with non-extracting screws and gaskets.
所述的衰减全反射光学测量平台,其所述测角转盘,在不需要测角的应用中,测角转盘以支杆套筒替代,支杆套筒下端面固接于固定圆盘上表面,支杆套筒中心轴线与轴的中心轴线重合;支杆套筒壁上正交设有支杆紧固螺钉,第二支杆插入支杆套筒内,与支杆套筒相对转动连接;旋转第二支杆使得支杆上端的测试部件被调节到适当位置后,由支杆紧固螺钉锁定第二支杆。In the attenuated total reflection optical measurement platform, the angle measurement turntable is replaced by a pole sleeve in the application that does not require angle measurement, and the lower end surface of the pole sleeve is fixed on the upper surface of the fixed disc. , the central axis of the pole sleeve coincides with the central axis of the shaft; the wall of the pole sleeve is orthogonally provided with a pole fastening screw, the second pole is inserted into the pole sleeve, and is connected with the pole sleeve for relative rotation; After rotating the second pole so that the test component at the upper end of the pole is adjusted to an appropriate position, the second pole is locked by the pole fastening screw.
所述的衰减全反射光学测量平台,其用于测试光波导导波特性、光波导传感器特性、表面等离子体共振传感器特性、薄膜样品厚度和折射率及光学特性;其中,The attenuated total reflection optical measurement platform is used for testing optical waveguide guide wave characteristics, optical waveguide sensor characteristics, surface plasmon resonance sensor characteristics, thin film sample thickness and refractive index and optical characteristics; wherein,
在用于表面等离子体共振传感器特性测试时,测试部件用棱镜全反射测试装置;When used for the surface plasmon resonance sensor characteristic test, the prism total reflection test device is used for the test part;
在用于测试薄膜样品厚度和折射率及光学特性时,测试部件用棱镜全反射测试装置,待测薄膜样品取代光传感芯片。When used to test the thickness, refractive index and optical properties of a film sample, the test component uses a prism total reflection test device, and the film sample to be tested replaces the light sensor chip.
本发明测量平台的优点在于:多个可动部件与固定部件组装在一起形成一个结构紧凑的小型化通用型测试系统,避免了分离器件在光路调节时操作困难、精度不高、稳定性和重复性差等缺点,该系统入射角准确可调,可灵活接收出射光,用途广泛、操作方便、测试精度高、重复性好。The advantage of the measurement platform of the present invention is that a plurality of movable parts and fixed parts are assembled together to form a compact and miniaturized general-purpose test system, which avoids the difficulty in operation, low precision, stability and repeatability of the separation device when adjusting the optical path. The system has the disadvantages of poor performance, the incident angle of the system can be adjusted accurately, and the outgoing light can be flexibly received. It has wide application, convenient operation, high test accuracy and good repeatability.
附图说明 Description of drawings
图1:本发明的一种衰减全反射光学测量平台的正视图;Fig. 1: the front view of a kind of attenuated total reflection optical measurement platform of the present invention;
图2:本发明的一种衰减全反射光学测量平台由底座、固定圆盘、固定臂、转动臂构成的主体结构的俯视图;Figure 2: A top view of the main structure of an attenuated total reflection optical measurement platform of the present invention consisting of a base, a fixed disc, a fixed arm, and a rotating arm;
图3:本发明的一种衰减全反射光学测量平台由底座、固定圆盘、固定臂、转动臂构成的主体结构的正视图;Figure 3: A front view of the main structure of an attenuated total reflection optical measurement platform of the present invention consisting of a base, a fixed disc, a fixed arm, and a rotating arm;
图4:本发明的一种基于反射光谱测量模式的衰减全反射光学测量平台的示意图;Figure 4: A schematic diagram of an attenuated total reflection optical measurement platform based on the reflection spectrum measurement mode of the present invention;
图5:利用图4所示的测量平台在入射角为8°,样品槽内注入去离子水和浓度为2μM的溶菌酶水溶液的条件下分别测得的表面等离子体共振(SPR)传感器的共振光谱图。Figure 5: Using the measurement platform shown in Figure 4, the resonance of the surface plasmon resonance (SPR) sensor was measured under the conditions of an incident angle of 8°, deionized water and a lysozyme aqueous solution with a concentration of 2 μM in the sample tank. Spectrum.
具体实施方式 Detailed ways
如图1、图2、图3和图4所示,是本发明的一种衰减全反射光学测量平台,图中,底座1、转动臂2、固定臂3、固定圆盘4、测角转盘5、测试部件6、光源7、探测器8、光纤9、支杆10、转动臂紧固螺钉21及螺钉支架22、轴23、不脱出螺钉24、滚动轴承25、螺钉31、支撑垫圈32、固定圆盘侧壁凹槽41、角度微调螺杆51、耦合棱镜61、光传感芯片62、样品槽63、压块64、横梁65、样品槽紧固螺钉66、底盘67、光源支架71、光纤宽带冷光源72、光纤准直器73和83、线性偏振器74、探测器支架81、光纤光谱仪82。As shown in Figure 1, Figure 2, Figure 3 and Figure 4, it is an attenuated total reflection optical measurement platform of the present invention, in the figure, the
固定臂3与固定圆盘4成一整体,转动臂2与固定圆盘4通过轴23、轴承25及不脱出螺钉24同轴转动连接,轴23与固定圆盘4经焊接等方式固定连接,转动臂2上设置有紧固螺钉21与螺钉支架22,固定圆盘4侧面刻有凹槽41,通过紧固螺钉21挤压凹槽41可锁定转动臂2;固定臂3与固定圆盘4置于底座1上方,并由至少三组螺钉31和支撑垫圈32与底座1固定连接,并使转动臂2位于底座1与固定圆盘4之间;测角转盘5置于固定圆盘4上方,测角转盘5底盘与固定圆盘4同轴固定连接,测角转盘5上设置有角度微调螺杆51,测试部件6通过自身支杆10固定在测角转盘5上转动盘中心开设的通孔内,光源7固定到光源支架71上,再通过支杆10将光源支架71固定到固定臂3上,探测器8固定到探测器支架81上,再通过支杆10将探测器支架81固定到转动臂2上,光源7、探测器8及测试部件6的中心到底座1上表面的距离相同,光源支架71和探测器支架81上分别设置有微调旋钮,光源光被耦合穿过测试部件6后可被探测器8接收;The fixed
固定圆盘4、固定臂3、转动臂2、底座1的连接关系也可以是:转动臂2与底座1转动连接;固定臂3与固定圆盘4成一整体,置于转动臂2上方,并由至少三组螺钉31和支撑垫圈32与底座1固定连接,并使固定圆盘4轴线与转动臂2连接轴轴线重合。The connection relation of fixed
测试部件6为光波导测试装置(发明专利,申请号:200910080063.1,申请日:2009年3月18日)、或光波导生化传感器测试装置(发明专利,申请号:200910093766.8,申请日:2009年9月28日)、或棱镜全反射测试装置。其中,测试部件自身支杆10在“光波导测试装置”专利申请书中对应于主支杆2,在“光波导生化传感器测试装置”专利申请书中对应于支架12。The test component 6 is an optical waveguide testing device (invention patent, application number: 200910080063.1, application date: March 18, 2009), or an optical waveguide biochemical sensor testing device (invention patent, application number: 200910093766.8, application date: September 2009 28th), or prism total reflection test device. Among them, the
棱镜全反射测试装置包括耦合棱镜61、光传感芯片62、样品槽63、底盘67、支杆10、及由压块64、横梁65和紧固螺钉66组成的样品槽紧固件。其中,耦合棱镜61为由高折射率玻璃或透光晶体制成的等腰直角棱镜,置于底盘67上方并用胶粘等方式使棱镜61的三角形底面与底盘67固定连接;横梁65置于底盘67上方,与底盘67固定连接,紧固螺钉66穿过横梁65中间的内螺纹孔与压块64连接,并使压块64朝向棱镜61镜面;支杆10置于底盘67下方并垂直固定在底盘67上,使支杆10的轴线与棱镜61的三角形底面一直角边垂直相交。光传感芯片62为SPR芯片或光波导芯片,样品槽63由硅橡胶或聚四氟乙烯材料制成;使用时,将少许耦合液滴在光传感芯片62背面,然后将芯片62背面紧贴棱镜61镜面,再将样品槽63紧贴光传感芯片62正面,通过旋转紧固螺钉66使样品槽63和光传感芯片62紧夹在棱镜61和压块64之间,并使样品槽63与光传感芯片62密封接触。The prism total reflection testing device includes a
光源7为小型化线偏振激光器或宽带线偏振平行光发射器。The light source 7 is a miniaturized linearly polarized laser or a broadband linearly polarized parallel light emitter.
探测器8为光电池或光敏二极管或光电倍增管或光纤光谱仪。当光源7为小型化线偏振激光器时,探测器8可选用光电池、光敏二极管及光电倍增管中的一种;当光源7为宽带线偏振平行光发射器时,探测器8选用光纤光谱仪。The
宽带线偏振平行光发射器包括一光纤宽带冷光源72,一光纤准直器73、一光源支架71、一线性偏振器74、及一光纤9,光纤一端与光纤宽带冷光源72固定连接,另一端与光纤准直器73固定连接,光纤准直器73固定到光源支架71上,线性偏振器74置于光纤准直器73前端,与光源支架71固定连接,并使从光纤准直器73射出的平行光垂直穿过偏振器,光源支架71通过支杆10垂直固定到固定臂3上,光源支架71上设置有微调旋钮,使从光纤准直器73射出的平行光束轴线与测角转盘5轴线垂直相交。The broadband linearly polarized parallel light transmitter includes a fiber broadband
当探测器8为光纤光谱仪82时,需要的附件包括:一光纤9和一光纤准直器83及一探测器支架81。光纤9一端与光纤光谱仪82固定连接,另一端与光纤准直器83固定连接,光纤准直器83固定到探测器支架81上,探测器支架81再通过支杆10垂直固定到转动臂2上,探测器支架81上设置有微调旋钮,从测试部件6输出的光束可被光纤准直器83接收并聚焦到光纤9端面,再由光纤9传输到光纤光谱仪82。When the
宽带线偏振平行光发射器使用的光纤与光纤光谱仪82使用的光纤通过分叉光纤结构互联形成三支四端的两头分叉光纤9,使得宽带线偏振平行光发射器的光纤准直器73通过分叉光纤同时与光纤冷光源72和光纤光谱仪82连接,光纤光谱仪82又通过分叉光纤同时与作为附件的光纤准直器83连接。The optical fiber used by the broadband linearly polarized parallel light transmitter and the optical fiber used by the
当入射平行光束照射到测试部件6的耦合棱镜61时,从棱镜61镜面反射的光束可通过调节测角转盘5使其沿原路返回并被光纤准直器73接收再由分叉光纤9传输到光纤光谱仪82进行探测,由此实现测试平台精确的光路准直与零度入射角定位功能。When the incident parallel light beam irradiates the
测角转盘5可以是手动操作的测角转盘,也可以电机驱动的全自动测角转盘。The
测角转盘5在不需测角的情况下可更换为支杆套筒,支杆套筒置于固定圆盘4上方并与固定圆盘4同轴固定连接,支杆套筒壁上设置有紧固螺钉,测试部件6通过自身支杆与支杆套筒转动连接,通过转动支杆使得测试部件6被调节到适当位置后再由紧固螺钉锁定。The angle-measuring
当衰减全反射光学测量平台用于测试分析光波导导波特性时,测试部件6选用光波导测试装置(发明专利,申请号:200910080063.1,申请日:2009年3月18日);当用于测试分析光波导传感器特性时,测试部件选用光波导生化传感器测试装置(发明专利,申请号:200910093766.8,申请日:2009年9月28日);当用于测试分析SPR传感器特性或波导模式谱特性时、测试部件6选用棱镜全反射测试装置;当用于测试分析薄膜样品的厚度和折射率及其光学特性时,测试部件6选用棱镜全反射测试装置,待测薄膜样品取代光传感芯片。When the attenuated total reflection optical measurement platform is used to test and analyze the characteristics of optical waveguide waveguide, the test component 6 selects the optical waveguide test device (invention patent, application number: 200910080063.1, application date: March 18, 2009); When testing and analyzing the characteristics of the optical waveguide sensor, the test component uses the optical waveguide biochemical sensor test device (invention patent, application number: 200910093766.8, application date: September 28, 2009); when used to test and analyze the characteristics of the SPR sensor or the waveguide mode spectral characteristics When, the test part 6 selects the prism total reflection test device for use; when used for testing and analyzing the thickness and the refractive index of the film sample and its optical properties, the test part 6 selects the prism total reflection test device for use, and the film sample to be tested replaces the light sensor chip.
实施例:Example:
底座1、转动臂2、固定臂3及固定圆盘4由不锈钢制成,底座1尺寸为195mm×100mm×12mm,转动臂2尺寸为80mm×25mm×12mm、固定臂3尺寸为45mm×20mm×12mm,固定圆盘4直径90mm,厚25mm;测角转盘5为市售手动旋转台,直径80mm,带有角度微调螺杆51;光纤宽带冷光源72为市售的卤钨灯光纤冷光源,光纤光谱仪82为市售的CCD(电荷耦合器件)光纤光谱仪,光谱测试范围300nm~1000nm,光谱分辨率1nm。两头分叉光纤为石英多模光纤,光纤芯直径600μm,光纤各端带有SMA光纤适配器。The
测试部件6采用棱镜全反射测试装置,装置底盘67、压块64、横梁65和紧固螺钉66由硬铝制成,耦合棱镜61由高折射率玻璃制成,棱镜的三角形底面边长为25mm×25mm×35.36mm,棱镜高为25mm。传感芯片62为SPR芯片,由BK7玻璃基板表面溅射45nm厚的金膜组成,芯片尺寸为25mm×20mm×1mm,样品槽63由硅橡胶块制成,样品槽尺寸为25mm×25mm×5mm,光耦合液为碘化亚甲基。The test part 6 adopts a prism total reflection test device, the
开启卤钨灯光纤冷光源72,从光纤射出的光透过光纤准直器73和偏振器74后成为线偏振平行光束,该光束照射到耦合棱镜61镜面被部分反射和部分折射,调节测角转盘5使得从棱镜61镜面反射的光束沿原路返回并被光纤准直器73接收,再由分叉光纤9传输到光纤光谱仪82,当光纤光谱仪探测到的光谱强度达到最大时停止调节测角转盘5并读取测角转盘指定的度数,该度数所对应的入射角为零度。之后再次调节测角转盘5,使得入射角设定为8°。然后旋转转动臂2并微调探测器支架81,使得从耦合棱镜61射出的衰减全反射光束被光纤准直器83接收并由分叉光纤9传输到光纤光谱仪82。当光纤光谱仪82探测到的光谱强度达到最大时,利用紧固螺钉21锁定转动臂2。Turn on the tungsten-halogen lamp optical fiber
利用移液器把去离子水注入样品槽63后纪录光纤光谱仪82探测到的反射光强度谱。然后把样品槽63中的去离子水更换为浓度为2μM的溶菌酶水溶液,同时以1s的时间间隔连续纪录反射光强度谱。当光谱被观测到不再随时间变化后,停止纪录。把溶液从样品槽63中取出后松开样品槽紧固件,更换新的SPR芯片,并取出样品槽63进行清洗以备下次使用。图5显示了在入射角为8°,样品槽内充满去离子水和浓度为2μM的溶菌酶水溶液的条件下分别测得的SPR传感器的共振光谱。每一光谱包括一波谷,波谷对应的波长即是SPP共振波长。溶菌酶在SPR芯片表面的吸附使得SPP共振波长相对于去离子水对应的SPP共振波长产生红移。Use a pipette to inject deionized water into the sample tank 63 and record the reflected light intensity spectrum detected by the
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