CN102988420B - Nano periplaneta americana product as well as preparation method and application thereof - Google Patents
Nano periplaneta americana product as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a nano periplaneta americana product as well as a preparation method and application thereof. The nano periplaneta americana product is a solid nano product consisting of 80 to 90 percent of periplaneta americana with the particle diameter of 20 to 80 nanometers. The preparation method for the nano periplaneta americana product comprises the following steps of: freezing, vacuum drying, crushing, ultrahigh-pressure homogenization, spray drying and the like on periplaneta americana. By utilizing the method, the average particle diameter of the periplaneta americana product reaches 20 to 80 nanometers, the specific surface area of the nano periplaneta americana product is enlarged, the quantity of surface atoms is increased, the dissolving-out speed and dissolving-out quantity of the active ingredients are increased, the curative effect is improved or the product consumption is reduced, the bioavailability is greatly improved, and the medicinal value is greatly improved.
Description
Technical field
The present invention relates to a kind of periplaneta americana goods, be specifically related to a kind of nanoscale periplaneta americana goods and its preparation method and application.
Background technology
Periplaneta americana, its diagnostic characteristics is adult ellipse, dorsoventrally compressed, body is yellowish-brown, rufous or dark brown, body surface gloss.Build is large, the long 25-45mm of body, and pronotary has a large pitchy butterfly patch, and there is one " T " shape yellow cord in the center line front of speckle, extands rearward to " little tail ", and the surrounding of speckle is lark, and trailing edge mottle is wider.Original producton location: the African torrid zone or subtropical zone, distribution in China present situation: all there is distribution all parts of the country, especially more common with south China area.
"Compendium of Materia Medica," said: "Cockroach, OK night, Fu grasshopper are three Southwest are eaten, mixed call vulgar and false negative disk drive for the woman, but also taboo lady called incense."Hanzhong and southwestern area are all desirable edible, and are nicknamed fragrant wife.As can be seen here, cockroach does medicinal existing long historical, and now for Kangfuxin Liquid patent medicine, existing also have the modern Chinese medicine decoction pieces such as periplaneta americana fine powder to occur.
The traditional Chinese medical science think periplaneta americana have " removing toxic substances disappear infantile malnutrition, inducing diuresis to remove edema, lifting immunity, improve the drug effect of weakness symptom and trauma care; conventionally adopt and catch and kill nuisanceless periplaneta americana high-temperature vapor at present; low-temperature reduced-pressure is dry, high-frequency vibration mill is pulverized fine powder for 200-300 micron as medicinal; In clinical trial, when this micron-sized periplaneta americana fine powder is used as Chinese medicine, the stripping quantity of active component is few, and dissolution rate is slow, need to use a large amount of medicines just can reach certain curative effect, causes the bioavailability of periplaneta americana fine powder very low.
Nanoscale science and technology is the new science and technology that phase late 1980s has just been born and has emerged, and its basic meaning is understanding and nature remodeling in nano-scale (0.1-100nm) scope.Need clinically to absorb nanoscale American-cockroach-extract better faster.Commonsense method is difficult to the activated nanoscale American-cockroach-extract of preparation.
Summary of the invention
The object of the present invention is to provide a kind of nano level periplaneta americana goods, in these goods, the particle diameter of the granule of 80-90%, within the scope of 20-80nm, can effectively improve curative effect and the bioavailability of periplaneta americana solid articles, and medical value is improved greatly.
Another object of the present invention is to provide a kind of preparation method of preparing above-mentioned nano level periplaneta americana goods, the method is not being destroyed under the prerequisite of periplaneta americana active component and structure, has solved the problem that adult bombay canary is prepared as to nanoscale periplaneta americana goods.
Another object of the present invention is to provide the application in medicine preparation as crude drug of nanoscale periplaneta americana goods, made its medicine of preparing there is disappear effect of infantile malnutrition, inducing diuresis to remove edema of promoting blood circulation to remove blood stasis, removing toxic substances; And use in WEIJIA and gather, infantile malnutrition, beriberi edema, furuncle, toxic swelling and snake bite and insect sting, wound repair, the treatment of the diseases such as primary hepatocarcinoma.
In order to reach first object of foregoing invention, the technical solution used in the present invention is: a kind of nanoscale periplaneta americana goods are provided, it is characterized in that: the solid nano product being formed the periplaneta americana of 20-80nm by the particle diameter of 80-90%, the specific surface area of these nanoscale periplaneta americana goods increases, surface atom number increases, the dissolution rate of active component wherein and stripping quantity increase, and have improved curative effect or have reduced the consumption of this product.
In order to reach second object of foregoing invention, the technical solution used in the present invention is: a kind of preparation method of nanoscale periplaneta americana goods is provided, it is characterized in that, comprise the following steps:
Freezing 3-6 hour under a, adult bombay canary is placed in-40-0 ℃ environment, then be positioned in vacuum desiccator, at 0-40 ℃, being dried to water content is 4-6%, obtains dry periplaneta americana product;
B, dried periplaneta americana product is crushed to 80-100 order, the water that adds 2-8 times of volume is made into suspension, under room temperature, on superhigh-voltage homogenizing machine, carries out extra high pressure homogenize with 110-150Mpa pressure, makes homogenizing fluid;
C, the homogenizing fluid making is sprayed at 0-40 ℃ dry, make periplaneta americana solid nano goods.
In step b, described pulverizing is that dry periplaneta americana product is placed in to efficient universal pulverizer, pulverizes with 3800-6000rpm/min, makes periplaneta americana powder.
In order to reach the 3rd object of foregoing invention, the technical solution used in the present invention is: the application in medicine preparation as crude drug of a kind of nanoscale periplaneta americana goods is provided.
The dosage form of described medicine can be pill, powder, tablet, suppository, granule, membrane, capsule, microcapsule, drop pill, aerosol, injection, unguentum, medicated wine, syrup or oral solution.
Described medicine be made by nanoscale periplaneta americana goods powder.
In sum, the particle diameter of most of granule of nanoscale periplaneta americana goods provided by the invention is at 20-80nm, this being refine to when nano level goods can make composition be utilized by human body reaches maximum utilization, greatly improves curative effect and the bioavailability of periplaneta americana goods; The preparation method of nanoscale periplaneta americana goods provided by the invention is not being destroyed under the prerequisite of periplaneta americana active component and structure, control the content of periplaneta americana high molecular weight protein by controlling the fineness of nanoscale pulverizing, make part high molecular weight protein be converted into polypeptide, directly, for human body utilization, improve drug effect; Product of the present invention can be used as the medicine of the various dosage forms of production of raw medicine, has disappear effect of infantile malnutrition, inducing diuresis to remove edema of promoting blood circulation to remove blood stasis, removing toxic substances; And use in WEIJIA gather, the treatment of the disease such as infantile malnutrition, beriberi edema, furuncle, toxic swelling, snake bite and insect sting, wound repair and primary hepatocarcinoma, medical value is greatly enhanced.
The specific embodiment
Below in conjunction with specific embodiment, the specific embodiment of the present invention is described in detail:
Embodiment 1
Take the adult bombay canary 100kg of GAP base cultivation, at-40 ℃, be placed in freezer freezing 3 hours, be positioned over again in vacuum desiccator, at 10 ℃, being dried to water content is 4%, make dry periplaneta americana product, drop into again in efficient universal pulverizer, under the high-speed stirred of 3800rpm/min, pulverize, cross 80 mesh sieves, add the water of 2 times of volumes to be made into suspension, under room temperature, in superhigh-voltage homogenizing machine, carry out extra high pressure homogenize with 110Mpa pressure, 4-5 circulation continuously, makes homogenizing fluid; The homogenizing fluid making is sprayed at 20 ℃ dry, obtain 96.6kg periplaneta americana nanometer products; Detect by JY/T001-1996 " transmission electron microscope method general rule ", in gained nanometer periplaneta americana goods, the particle diameter of 85% microgranule, within the scope of 20-80nm, meets the science regulation of nanoparticle completely.
Using the above-mentioned periplaneta americana goods that make as crude drug, through mixing, granulate, dry, be pressed into 10000 tablets of nanometer periplaneta americana tablets, this nanometer periplaneta americana tablet has disappear effect of infantile malnutrition, inducing diuresis to remove edema of promoting blood circulation to remove blood stasis, removing toxic substances; And use in WEIJIA gather, the treatment of the disease such as infantile malnutrition, beriberi edema, furuncle, toxic swelling, snake bite and insect sting, wound repair and primary hepatocarcinoma.
Embodiment 2
Take the adult bombay canary 200kg of GAP base cultivation, at-20 ℃, be placed in freezer freezing 4 hours, be positioned over again in vacuum desiccator, at 20 ℃, being dried to water content is 5%, make dry periplaneta americana product, drop into again in efficient universal pulverizer, under the high-speed stirred of 4500rpm/min, pulverize, cross 90 mesh sieves, add the water of 5 times of volumes to be made into suspension, under room temperature, in superhigh-voltage homogenizing machine, carry out extra high pressure homogenize with 130Mpa pressure, 4-5 circulation continuously, makes homogenizing fluid; The homogenizing fluid making is sprayed at 30 ℃ dry, obtain 194kg periplaneta americana nanometer products; Detect by JY/T001-1996 " transmission electron microscope method general rule ", in gained nanometer periplaneta americana goods, the particle diameter of 88% microgranule, within the scope of 20-80nm, meets the science regulation of nanoparticle completely.
Using the above-mentioned periplaneta americana goods that make as powder, this nanometer periplaneta americana powder has disappear effect of infantile malnutrition, inducing diuresis to remove edema of promoting blood circulation to remove blood stasis, removing toxic substances; And use in WEIJIA gather, the treatment of the disease such as infantile malnutrition, beriberi edema, furuncle, toxic swelling, snake bite and insect sting, wound repair and primary hepatocarcinoma.
Embodiment 3
Take the adult bombay canary 150kg of GAP base cultivation, at-5 ℃, be placed in freezer freezing 6 hours, be positioned over again in vacuum desiccator, at 40 ℃, being dried to water content is 6%, make dry periplaneta americana product, drop into again in efficient universal pulverizer, under the high-speed stirred of 4000rpm/min, pulverize, cross 100 mesh sieves, add the water of 8 times of volumes to be made into suspension, under room temperature, in superhigh-voltage homogenizing machine, carry out extra high pressure homogenize with 150Mpa pressure, 4-5 circulation continuously, makes homogenizing fluid; The homogenizing fluid making is sprayed at 40 ℃ dry, obtain 145kg periplaneta americana nanometer products; Detect by JY/T001-1996 " transmission electron microscope method general rule ", in gained nanometer periplaneta americana goods, the particle diameter of 95% microgranule, within the scope of 20-80nm, meets the science regulation of nanoparticle completely.
Using the above-mentioned periplaneta americana goods that make as powder, this nanometer periplaneta americana powder has disappear effect of infantile malnutrition, inducing diuresis to remove edema of promoting blood circulation to remove blood stasis, removing toxic substances; And use in WEIJIA gather, the treatment of the disease such as infantile malnutrition, beriberi edema, furuncle, toxic swelling, snake bite and insect sting, wound repair and primary hepatocarcinoma.
Embodiment 4
Take the adult bombay canary 300kg of GAP base cultivation, at 0 ℃, be placed in freezer freezing 5 hours, be positioned over again in vacuum desiccator, at 15 ℃, being dried to water content is 5%, make dry periplaneta americana product, drop into again in efficient universal pulverizer, under the high-speed stirred of 6000rpm/min, pulverize, cross 100 mesh sieves, add the water of 7 times of volumes to be made into suspension, under room temperature, in superhigh-voltage homogenizing machine, carry out extra high pressure homogenize with 120Mpa pressure, 4-5 circulation continuously, makes homogenizing fluid; The homogenizing fluid making is sprayed at 25 ℃ dry, obtain 290kg periplaneta americana nanometer products; Detect by JY/T001-1996 " transmission electron microscope method general rule ", in gained nanometer periplaneta americana goods, the particle diameter of 82% microgranule, within the scope of 20-80nm, meets the science regulation of nanoparticle completely.
Using the above-mentioned periplaneta americana goods that make as crude drug, be placed in general ball pot and carry out general system, make the pill of 0.5-1mm, this nanometer periplaneta americana pill has disappear effect of infantile malnutrition, inducing diuresis to remove edema of promoting blood circulation to remove blood stasis, removing toxic substances; And use in WEIJIA gather, the treatment of the disease such as infantile malnutrition, beriberi edema, furuncle, toxic swelling, snake bite and insect sting, wound repair and primary hepatocarcinoma.
Reference embodiment 1:
The preparation of American-cockroach-extract preparation:
A, coarse crushing: dry periplaneta americana is pulverized, make periplaneta americana coarse powder, for subsequent use;
B, immersion: add the water of 3 ~ 5 times of amounts according to the coarse powder of every 100kg, under the temperature conditions of 70 ~ 78 ℃, soak and make coarse powder mixed liquor in 0.5 ~ 2 hour, for subsequent use;
C, three extractions: coarse powder mixed liquor adopts the method decocting to carry out three times and extracts, and when coarse powder mixed liquor is extracted for the first time, extraction time is 7 ~ 9 hours; Then after adding the water of remaining 2 ~ 4 times of amounts of liquid in the remaining liquid after extracting for the first time, extract for the second time, extraction time is 5 ~ 7 hours; More than adding in the most backward remaining liquid after extracting for the second time, after the water of 2 ~ 4 times of amounts of liquid, extract for the third time, extraction time is 3 ~ 5 hours, makes respectively the extracting solution of three times, for subsequent use;
D, filtration for the first time: the extracting solution of three times is merged and mixed, then filter and make one-level filtrate, for subsequent use;
E, concentrated for the first time: the one-level concentrated solution that when one-level filtrate is concentrated into 70 ~ 90 ℃, relative density is 1.05 ~ 1.25, for subsequent use;
F, alcoholization are processed: be the ethanol of 93 ~ 97%w/w to adding gradually concentration in one-level concentrated solution, and fully stir, make it contain alcohol amount and reach till 45 ~ 55%, under the temperature conditions of 70 ~ 90 ℃, be incubated 20 ~ 40 minutes, then leave standstill 10 ~ 14 hours, make solution of alcoholized, for subsequent use;
G, filtration for the second time: solution of alcoholized is discarded to upper strata oils and fats, and lower floor's medicinal liquid filters and makes secondary filtrate, for subsequent use;
H, concentrated for the second time: secondary filtrate is carried out after ethanol recycling, the secondary concentrated solution that while being concentrated into 70 ~ 90 ℃, relative density is 1.15 ~ 1.30, for subsequent use;
I, unguentum preparation: secondary concentrated solution is concentrated under condition of normal pressure to the unguentum of moisture≤25 ~ 35%, obtains American-cockroach-extract-containing, for subsequent use;
Patent medicine: according to effective ingredient mixture: the weight ratio of purified water: glycerol=1000g:800 ~ 1200ml:4 ~ 6ml adds purified water and glycerol in effective ingredient mixture, stir, through plate-and-frame filtration, filtrate was boiled after 30 minutes, be distributed into 100ml/ bottle or 50ml/ bottle, through autoclave sterilization sterilization, be chilled to room temperature again, obtain patent medicine.
Test example 1
The nanometer periplaneta americana goods of preparing take embodiment 1,2,3,4 are as powder.
Above-mentioned powder is used for the treatment of the clinical testing data of dissipating blood stasis for subsidence of swelling disease:
One, patient's classified data:
Dissipating blood stasis for subsidence of swelling patient 150 examples, meet diagnostic criteria.Be divided into immediately treatment group and matched group, treatment group 100 examples, male's 53 examples, women's 47 examples, 16 ~ 40 years old age; Matched group 50 examples, male's 25 examples, women's 25 examples, 16 ~ 40 years old age.Two groups of patient ages, sex and courses of disease are learned processing by statistics, and no significant difference, has comparability.
Two, Therapeutic Method:
Treatment group: getting the nanometer periplaneta americana goods that make according to preparation method in embodiment 1,2,3,4 is powder, it is oral that the good doctor in Sichuan climbs Western medicine industry Co., Ltd, a 2-5g; 3 times on the one; Take continuously 1 month;
Matched group 1: YUNNAN BAIYAO DING, Yunnan Paiyao Group Corp., Ltd produces, oral, a 3 ~ 5ml, 3 times on the one; Take continuously 1 month.
Matched group 2: American-cockroach-extract preparation, produce according to reference embodiment 1, oral, a 10ml, 3 times on the one; Take continuously 1 month.
Three, curative effect comparative result:
Table 1 is depicted as six groups of curative effect comparisons, wherein treatment group 100 examples, and matched group 50 examples, compare respectively cure rate, effective percentage, inefficiency, total effective rate.Six groups of 15 days cure rates and 1 month cure rate shown in table 2.(n number of delegates in table 1 and table 2)
Judgment criteria:
Cure: without ecchymosis, without swelling, without pain;
Effective: pain is light, can recognize, have a rest but do not affect routine work, part is slightly livid purple, and take purple as main, area is less than 1cm × 1cm; Swelling is light, and the increase of all footpaths is less than 5%;
Invalid: pain weight, swelling weight, the increase of all footpaths is greater than 10%.Local livid purple obvious, take green grass or young crops as main, area is greater than 4cm × 4cm.
Table 1:
Table 2:
| Group | n | 15 days total effective rates (%) | 1 month total effective rate (%) |
| Treatment group 1 | 25 | 42 | 92 |
| Treatment group 2 | 25 | 45 | 96 |
| Treatment group 3 | 25 | 48 | 98 |
| Treatment group 4 | 25 | 42 | 88 |
| Matched group 1 | 25 | 35 | 82 |
| Matched group 2 | 25 | 38 | 86 |
Four, conclusion (of pressure testing):
Six groups of curative effect comparisons shown in table 1, treatment group total effective rate is respectively 92%, 96%, and 98%, 88%; Matched group total effective rate is respectively 82%, 86%.Shown in table 2, six groups of 15 days total effective rates are respectively treatment group 42%, 45%, 48%, 42%, and matched group is respectively 35%, 38%.As can be seen here, treatment group curative effect is all better than matched group.Preparation of the present invention is better than traditional handicraft preparation aspect dissipating blood stasis for subsidence of swelling, this preparation can directly act on focus, improve cell wall breaking rate, specific surface area, molecular weight little, significantly improve dissolution rate and body internal absorption factor and the bioavailability of its active component, thereby reduce dosage and improve therapeutic effect; In addition, the mouthfeel of this powder is better, and health more, owing to having improved medicament absorbance and utilization rate, has also reduced cost accordingly, and then has reduced patient and use threshold, clinic recommendation.
Powder take nanometer periplaneta americana goods as active component provided by the invention, although in conjunction with specific embodiments the specific embodiment of the present invention is described in detail, is not the restriction to this patent protection domain.In claims limited range, the various modifications that those skilled in the art can make without creative work or adjustment are still subject to the protection of this patent.
Claims (6)
1. a preparation method for nanoscale periplaneta americana goods, is characterized in that, comprises the following steps:
Freezing 3-6 hour under a, adult bombay canary is placed in-40-0 ℃ environment, then be positioned in vacuum desiccator, at 10-40 ℃, being dried to water content is 4-6%, obtains dry periplaneta americana product;
B, dried periplaneta americana product is crushed to 80-100 order, the water that adds 2-8 times of volume is made into suspension, under room temperature, on superhigh-voltage homogenizing machine, carries out extra high pressure homogenize with 110-150Mpa pressure, makes homogenizing fluid;
C, the homogenizing fluid making is sprayed at 20-40 ℃ dry, make the solid nano product being formed the periplaneta americana of 20-80nm by the particle diameter of 80-90%.
2. the preparation method of nanoscale periplaneta americana goods according to claim 1, is characterized in that: in step b, described pulverizing is that dry periplaneta americana product is placed in to efficient universal pulverizer, pulverizes with 3800-6000 rpm, makes periplaneta americana powder.
3. the prepared nanoscale periplaneta americana of the preparation method goods of nanoscale periplaneta americana goods according to claim 1 and 2.
4. nanoscale periplaneta americana goods claimed in claim 3 application in medicine preparation as crude drug.
5. nanoscale periplaneta americana goods according to claim 4 application in medicine preparation as crude drug, is characterized in that: the dosage form of described medicine is pill, powder, tablet, suppository or granule.
6. nanoscale periplaneta americana goods according to claim 4 application in medicine preparation as crude drug, is characterized in that: described medicine is the powder of being made up of nanoscale periplaneta americana goods.
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| CN105943558A (en) * | 2016-06-21 | 2016-09-21 | 四川好医生攀西药业有限责任公司 | Application of periplaneta americana in preparation of drug for preventing and treating radiation-induced damage |
| CN106539690B (en) * | 2015-09-18 | 2020-08-04 | 天士力医药集团股份有限公司 | Continuous intelligent preparation method of liquid cooling dropping pills |
| CN107684563B (en) * | 2016-12-07 | 2020-08-25 | 四川好医生攀西药业有限责任公司 | Medicine containing periplaneta americana and imatinib and application thereof |
| CN107693546A (en) * | 2016-12-16 | 2018-02-16 | 四川好医生攀西药业有限责任公司 | A kind of medicine containing American cockroach and Sorafenib and its application |
| CN107582571A (en) * | 2017-04-10 | 2018-01-16 | 昆明赛诺制药股份有限公司 | A kind of medicine for treating tinea pedis and preparation method thereof |
| CN108785330B (en) * | 2018-06-26 | 2022-07-29 | 广州博济堂医药保健有限公司 | Application of periplaneta americana extract in preparation of medicine for treating wound healing |
| CN117503801B (en) * | 2023-11-07 | 2024-07-26 | 济南三源药业有限公司 | American cockroach powder for promoting milk secretion and preparation method thereof |
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| CN1255117C (en) * | 2003-04-30 | 2006-05-10 | 严少宏 | Medicinal periplaneta americana extracturm extracting process |
| CN2794622Y (en) * | 2005-05-11 | 2006-07-12 | 西安量维生物纳米科技股份有限公司 | Low temperature jet flow spray drier |
| CN101254212B (en) * | 2008-03-25 | 2010-11-17 | 浙江大学 | Use of valid part of periplaneta American extract in preparing medicine for HSV |
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Effective date of registration: 20200818 Address after: 615000 Liangshan Yi Autonomous Prefecture, Xichang province Sichuan Airport Road Patentee after: SICHUAN GOODDOCTOR PANXI PHARMACEUTICAL Co.,Ltd. Address before: 13, building 88, building B, four Wei building, No. 610031 Camp Road, Sichuan, Chengdu Patentee before: Geng Funeng |