CN102888341B - Compound bacteria and application in biological bacteria liquid pulping technology of compound bacteria - Google Patents
Compound bacteria and application in biological bacteria liquid pulping technology of compound bacteria Download PDFInfo
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- CN102888341B CN102888341B CN2012103427048A CN201210342704A CN102888341B CN 102888341 B CN102888341 B CN 102888341B CN 2012103427048 A CN2012103427048 A CN 2012103427048A CN 201210342704 A CN201210342704 A CN 201210342704A CN 102888341 B CN102888341 B CN 102888341B
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention relates to compound bacteria and application of the compound bacteria in a biological bacteria liquid pulping technology. The compound bacteria comprise bacillus stored with a number of CGMCC No. 5971, rheinheimera tangshanensis stored with a number of CGMCC No.5972, acinetobacter lwoffii stored with a number of CGMCC No. 5973, and pseudomonas fluorescens stored with a number of CGMCC No. 5974. The method mainly comprises the folllowing steps of: preparing bacteria liquid; processing raw materials; and pulping, wherein pulping steps comprise the following steps of: defibering, carrying out biodegradation, sterilizing through steam, carrying out rough pulping, carrying out fine pulping, screening a sizing agent, removing latency, and washing the sizing agent. A paperboard prepared by a biological bacteria liquid pulping method provided by the invention reaches a level over AA grade; waste liquid produced in a pulping process can be recycled or transformed into methane which can be served as an energy resource or organic fertilizer, and therefore, energy sources are saved, and environment protection is achieved.
Description
Technical field
The present invention relates to a kind of biological bacteria liquid pulping method, relate in particular to a kind of composite flora and biological bacteria liquid pulping method thereof.
Background technology
Pulping technique is exactly with raw materials such as the stalk of plant, branch, trunks, by degraded (or dissociating), removes wherein xylogen and the material such as pectin, obtains the process of fibrous matter.Fiber is the main component of papermaking.
In pulping process, isolate fiber, at first will be these non-fibrous material strippings, obtaining fiber is exactly the slurrying purpose.
The present situation of slurrying industry present according to China, mainly occupy an leading position with chemical pulp in being applied to produce, the waste liquor contamination sublimity that produces during chemical pulp is produced, damage to land, polluted air; Add a large amount of sodium hydroxide (caustic soda) in slurrying, also use chemical harmful element in bleaching process, producing waste liquid can't all recycle.And it is high to consume energy, and power consumption and water consumption are large; , all using a large amount of electricity, cause and become the power consumption rich and influential family in whole production process.The waste liquid blowdown flow rate is large; The waste liquid that can't recycle can only outwards discharge.Do not meet national energy-saving and reduce discharging policy.Material can not reach the Efficient Cycle recycling.Chemicals can't separate from waste liquid, organism and chemicals mix, and organism also can't be reused, and causes a large amount of losses.
Therefore, be necessary to develop the bio-pulping technology, fundamentally solve the pollution difficult problem of pulping and papermaking industry.Although the bio-pulping of employing technology is also arranged in prior art, but the biological bacteria that usually adopts is whiterot fungi or actinomycetes, although it makes moderate progress with respect to traditional chemical pulping technology, the defect that but the fiber fineness that still have long reaction time, makes is low, yield is low, be difficult to be applied to actual production.Therefore, still need the further new biological bacteria of exploitation, improve bio-pulping process, to overcome above-mentioned defect, and realize simultaneously energy-saving and emission-reduction, water-saving, reduce production costs and improve the rate of utilization of material.
Summary of the invention
The objective of the invention is to overcome the chemical pulping method of prior art and the above-mentioned defect of bio-pulping method, a kind of new slurrying biological bacteria is provided.The contriver, by long screening operation, has obtained to be suitable for the biological bacteria that can realize the foregoing invention purpose of bio-pulping.
Particularly, the invention provides a kind of composite flora, it comprises the genus bacillus that preserving number is CGMCC No.5971 (Bacillus sp.), preserving number is that the silent Salmonella (Rheinheimera tangshanensis) of Tangshan Lay mattress, the preserving number of CGMCC No.5972 is the acinetobacter lwoffii (Acinetobacter lwoffii) of CGMCC No.5973, the Pseudomonas fluorescens (Pseudomonas fluorescens) that preserving number is CGMCC No.5974.
Described composite flora can be used in the slurrying of biological bacteria liquid.
The present invention also provides a kind of biological bacteria liquid pulping method, and it mainly comprises step:
1) configuration of bacterium liquid: above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 1-3:1-3:1-2:1-2;
2) raw material is processed: with segment or section after woody raw material peeling, or the draft raw material is cut into chunks, the raw material that cuts is put into the fermentation vat swollen;
3) slurrying, it comprises step:
Discongest: with the raw material thread rolling of swollen and/or crowded the stone roller;
Biological degradation: the raw material soaking after discongesting is in the bacterium liquid that configures;
Wet sterilization: with the raw material after above-mentioned biological degradation pull out from bacterium liquid, draining, pass into water vapor sterilizing;
Corase grind slurry: the raw material after sterilizing is carried out one section corase grind slurry, become fibrous bundle;
Fine grinding slurry: above-mentioned one section corase grind slurry is carried out two sections fine grinding slurries, make fibrous bundle disperse into single fiber;
Slurry screening: screening, filter the fibrous bundle in the slurry after one section corase grind slurry and two sections fine grindings slurries, defibrination makes it make single fiber again;
Latent, pulp washing disappears: the above-mentioned paper pulp that makes is soaked in warm water, be used for cardboard processed.
The density of the bacterium liquid that wherein, forms in step 1) is 6 * 10
7Individual/more than the ml bacterium.
Wherein, step 2) in, the woody raw material length that cuts is 3-4cm, the draft raw material length that cuts is 4-5cm.The described swollen time is 10-12h.
Wherein, the described biological degradation temperature of step 3) remains on 35-40 ℃, time 30-34 hour.The mass ratio of the raw material after discongesting and bacterium liquid is 1:6-8.Described steam sterilizing is water at atmospheric pressure steam sterilizing 10-30 minute.
Further, step 2) soak soak solution after raw material in through flocculation, precipitation, the supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas.
The advantage of biological bacteria liquid pulping method provided by the invention is: 1) free from environmental pollution: waste liquid directly changes into organic fertilizer, reaches zero release, and zero pollutes.2) bio-pulping can play a protective role to fiber, with traditional chemical process, compares, and present method can all reclaim full fiber and half fiber, has therefore improved slurry yield.Reduce energy consumption 30% than traditional slurrying; Paper pulp yield improves 20-25%.3) bio-pulping is to carry out lignin degradation under normal pressure, energy-conservation, reduction of discharging, low-carbon (LC).4) production cost is low, and economic benefit is high.Adopt the cardboard of this biological bacteria liquid pulping method preparation to reach the above level of AA level.
Byproduct of the present invention is transported to settling tank flocculation, precipitation, and supernatant liquor returns to second stage employ, remakes presoak and uses.Contain gas chromatography and the nutrient for plants such as N, P, K such as abundant xylogen in throw out, throw out mixes with old bacterium liquid (the raw material thickness bacterium liquid of repeatedly degrading, also contain N, P, K, Fe and trace element) again, acidifying, then together enter in marsh gas fermentation pool, produce biogas.With the Boiler Ash mixing granulation of natural pond slag, natural pond liquid and pulverizing, the granulation fertilizer, dispatch from the factory finally, realizes zero release.
The biological flora of the present invention by providing above-mentioned contriver to obtain through long creation work, further perfect existing bio-pulping technology, reduce the reaction times, improved the purity and the yield that make fiber, made this technology to apply on a large scale in actual production.Applying biological bacterium of the present invention at short notice in the degrading plant body xylogen produce paper pulp, papermaking, the byproduct twice transformation becomes biogas, biogas coal supply, gas boiler for dual purpose combustion heating are saved coal consumption.Finally, the natural pond slag is made organic fertilizer, has formed the economic circulation new model of " material organic transformation ", reaches the no waste discharging, namely zero release.Fundamentally solved the pollution difficult problem of pulping and papermaking industry.Energy-saving and emission-reduction, water-saving, reduced production cost, improved the rate of utilization of material.
For above and other objects of the present invention, feature and advantage can be become apparent, preferred embodiment cited below particularly, and coordinate accompanying drawing, be described in detail below.
Description of drawings
Fig. 1 is the schema according to the biological bacteria liquid pulping method of one embodiment of the invention;
Fig. 2 is the schema of biological bacteria liquid pulping method according to another embodiment of the present invention.
Embodiment
The configuration of embodiment 1 bacterium liquid
the biological flora that adopts in the present invention on April 6th, 2012 at China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City) preservation, it comprises the genus bacillus that preserving number is CGMCC No.5971 (Bacillus sp.), preserving number is the silent Salmonella (Rheinheimera tangshanensis) of the Tangshan Lay mattress of CGMCC No.5972, preserving number is the acinetobacter lwoffii (Acinetobacter lwoffii) of CGMCC No.5973, preserving number is the Pseudomonas fluorescens (Pseudomonas fluorescens) of CGMCC No.5974.
Above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 1-3:1-3:1-2:1-2; The density of the bacterium liquid that forms is 6 * 10
7Individual/more than the ml bacterium, standby.
The biological bacteria liquid slurrying of embodiment 2 woody raw materials
, take caragana microphylla as raw material, illustrate the biological bacteria liquid pulping method while adopting woody raw material.All the other woody raw materials, for example the bio-pulping method of willow, eucalyptus can be carried out with reference to this technique.
While wherein adopting caragana microphylla, composite flora is configured according to following mass ratio:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 3:1:2:2;
While adopting willow, composite flora is configured according to following mass ratio:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 3:2:1:2;
While adopting eucalyptus, composite flora is configured according to following mass ratio:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 2:2:1:2.
Specifically please refer to Fig. 1, the flow process of biological bacteria liquid pulping method is divided into three phases: preparatory stage, slurrying workshop section and byproduct workshop section.
(1) preparatory stage: 1-4
1)-2): the caragana microphylla that harvesting is returned carries out the separation of skin bar.Adopt winnowing machine or other mechanical separation all can.After the skin bar separates, skin is inputted organic feed workshop processing feed, the bar after decortication is inputted next program.
3) with bar segment on cutting machine, length is 3-4cm, and angle is for well, to increase infiltrating area.
4) the branch section input after peeling soak in storehouse or fermentation vat wash, cold soaking, at first the foreign material such as raw material appearance earth are washed away, soak simultaneously, water temperature is natural temperature, the time soaking into, swollen is as the criterion, 10-12h.After liquid muddiness after repeatedly soaking, after flocculating, precipitating, supernatant liquor can also reuse.The fermentation of throw out input methane-generating pit, produce biogas.
Above four steps can be that intermittent type can be also linkage type.
(2) slurrying workshop section: 5-12
5) discongest: with the branch section input thread-rolling machine of swollen or squeeze grind, kneading machine carrys out the innovation wood segment structure, makes it loose one-tenth wood wool shape, is conducive to the biological bacteria infiltration, brings into play its Degradation.
6) biological degradation: in raw material input biological bacteria degraded storehouse or tank after discongesting, be immersed in the bacterium liquid of embodiment 1 preparation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:6, and temperature remains on 35-40 ℃, time 30-34 hour.The xylogen degradation reaction occurs under the condition of biological bacteria, bring into play its single-minded effect.
7) wet sterilization: after raw materials pretreatment is completed, with raw material pull out from bacterium liquid, draining, be input in the steam storehouse, pass into water at atmospheric pressure steam 10-30 minute sterilizing.Paste roller mill is inputted with raw material in lower end.
8) corase grind slurry:, with carrying out one section corase grind slurry in the input of the raw material after sterilizing high-density paste mill, become coarse fiber bundle.
9) fine grinding slurry: enter again high-density paste mill from the coarse particles of above-mentioned output and carry out the fine grinding slurry, make fibrous bundle disperse into single fiber.
10) slurry screening: in the slurry after two sections defibrinations, contain the minority fibrous bundle, need through screening, filter tow, defibrination has reached single fiber again.
11) latent, the pulp washing that disappears: the paper pulp after corase grind slurry and fine grinding slurry, be subjected to mechanical friction, major part is bended, torsional deformation, soaks the fiber deflection that the elimination defibrination causes in warm water, makes it to unfold.
12) cardboard processed: with the pulping liquor of above-mentioned preparation input paper machine, cardboard processed after copying the technical process such as paper.
(3) byproduct stage: A-C
In A, 3, isolated caragana microphylla skin contains abundant nutritive ingredient, and fermentation changes into ox, sheep feed.
After liquid muddiness through repeatedly soaking, washing in B, 4, through flocculation, precipitation, the supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas, passes into the coal gas boiler for dual purpose and does fuel, can play effect of energy.
C, biological organic fertilizer: natural pond slag, natural pond liquid after the methane-generating pit fermentation are the biological organic fertilizers that enriches, and liquid topdresses as farm crop and flower nutrient solution uses, and the solid granulation is made base manure and used, and is all green fertilizers.
After defibrination, paper physical property index determining result please see following table 1 for details, and every data have all reached excellent fluting board AA level level (table 3):
Table 1
The biological bacteria liquid slurrying of embodiment 3 draft raw materials
, take wheat straw as raw material, illustrate the biological bacteria liquid pulping method while adopting the draft raw material.All the other draft raw materials, for example the bio-pulping method of wheat straw, straw, cornstalk can be carried out with reference to this technique.
While wherein adopting wheat straw, composite flora is configured according to following mass ratio:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 2:2:1:2;
While adopting straw, composite flora is configured according to following mass ratio:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 1:3:1:2;
While adopting cornstalk, composite flora is configured according to following mass ratio:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 2:2:2:1.
Specifically please refer to Fig. 2, the flow process of biological bacteria liquid pulping method is divided into three phases: preparatory stage, slurrying workshop section and byproduct workshop section.
(1) preparatory stage: 1,4
Wheat straw is cut into the segment of 4-5cm, input soak in storehouse or fermentation vat wash, cold soaking, at first the foreign material such as raw material appearance earth are washed away, soak simultaneously, water temperature is natural temperature, the time soaking into, swollen is as the criterion, 10-12h.After liquid muddiness after repeatedly soaking, after flocculating, precipitating, supernatant liquor can also reuse.The fermentation of throw out input methane-generating pit, produce biogas.
(2) slurrying workshop section: 5-12 and (three) byproduct stage: A-C are with embodiment 2, and the biological bacteria of wherein using such as embodiment 1 do not repeat them here, and wherein during biological degradation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:8.
After defibrination, paper physical property index determining result please see following table 2 for details.
Table 2
The quality index of table 3 Corrugating Base Paper
Although the present invention discloses as above with preferred embodiment; so it is not in order to limit the present invention; any person of ordinary skill in the field; without departing from the spirit and scope of the present invention; when doing a little change and improvement, so protection scope of the present invention is as the criterion when looking the claim person of defining.
Claims (7)
1. composite flora, it comprises the genus bacillus that preserving number is CGMCC No.5971 (Bacillus sp.), preserving number is that the silent Salmonella (Rheinheimera tangshanensis) of Tangshan Lay mattress, the preserving number of CGMCC No.5972 is the acinetobacter lwoffii (Acinetobacter lwoffii) of CGMCC No.5973, the Pseudomonas fluorescens (Pseudomonas fluorescens) that preserving number is CGMCC No.5974, above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 1-3:1-3:1-2:1-2; The density of the bacterium liquid that forms is 6 * 10
7Individual/more than the ml bacterium.
2. the application of composite flora claimed in claim 1 in the slurrying of biological bacteria liquid.
3. a biological bacteria liquid pulping method, is characterized in that, comprises step:
1) configuration of bacterium liquid: composite flora claimed in claim 1 is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: acinetobacter lwoffii: Pseudomonas fluorescens is 1-3:1-3:1-2:1-2, and the density of bacterium liquid is 6 * 10
7Individual/more than the ml bacterium;
2) raw material is processed: with section after woody raw material peeling, or the draft raw material is cut into chunks, the raw material that cuts is put into the fermentation vat swollen;
3) slurrying, it comprises step:
Discongest: with the raw material thread rolling of swollen and/or crowded the stone roller;
Biological degradation: the raw material soaking after discongesting is in the bacterium liquid that configures, and the mass ratio of the raw material after discongesting and bacterium liquid is 1:6-8, and degradation temperature is 35-40 ℃, time 30-34 hour;
Wet sterilization: with the raw material after above-mentioned biological degradation pull out from bacterium liquid, draining, pass into water vapor sterilizing;
Corase grind slurry: the raw material after sterilizing is carried out one section corase grind slurry, become fibrous bundle;
Fine grinding slurry: above-mentioned one section corase grind slurry is carried out two sections fine grinding slurries, make fibrous bundle disperse into single fiber;
Slurry screening: screening, filter the fibrous bundle in the slurry after one section corase grind slurry and two sections fine grindings slurries, defibrination makes it make single fiber again;
Latent, pulp washing disappears: the above-mentioned paper pulp that makes is soaked in warm water, be used for cardboard processed.
4. biological bacteria liquid pulping method according to claim 3, is characterized in that step 2) in, the woody raw material length that cuts is 3-4cm, the draft raw material length that cuts is 4-5cm.
5. according to claim 3 or 4 described biological bacteria liquid pulping methods, is characterized in that step 2) the described swollen time is 10-12h.
6. biological bacteria liquid pulping method according to claim 3, is characterized in that, the described steam sterilizing of step 3) is water at atmospheric pressure steam sterilizing 10-30 minute.
7. biological bacteria liquid pulping method according to claim 3, is characterized in that step 2) in soak soak solution after raw material through flocculation, precipitation, the supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas.
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| CN2012103427048A CN102888341B (en) | 2012-09-17 | 2012-09-17 | Compound bacteria and application in biological bacteria liquid pulping technology of compound bacteria |
| US14/428,963 US20150247116A1 (en) | 2012-09-17 | 2013-09-16 | Rheinheimera tangshanensis CGMCC#5972 and Its Application Thereof |
| PCT/CN2013/001084 WO2014040383A1 (en) | 2012-09-17 | 2013-09-16 | Biological bacterial solution and application thereof |
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| CN108221431A (en) * | 2016-12-14 | 2018-06-29 | 康盛时代（北京）生物技术有限公司 | A kind of technique for crudefiber crop lignin of degrading and application thereof |
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| CN202247507U (en) * | 2011-09-27 | 2012-05-30 | 天津广聚源纸业有限公司 | Biological pulping system for papermaking production |
| CN102559541A (en) * | 2011-12-09 | 2012-07-11 | 中国农业科学院麻类研究所 | Method for quickly extracting herbaceous fibers in factory fermentation mode by using full-function strains |
| CN102619119A (en) * | 2012-02-24 | 2012-08-01 | 王福喜 | Biological accumulation pulping method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN202247507U (en) * | 2011-09-27 | 2012-05-30 | 天津广聚源纸业有限公司 | Biological pulping system for papermaking production |
| CN102559541A (en) * | 2011-12-09 | 2012-07-11 | 中国农业科学院麻类研究所 | Method for quickly extracting herbaceous fibers in factory fermentation mode by using full-function strains |
| CN102619119A (en) * | 2012-02-24 | 2012-08-01 | 王福喜 | Biological accumulation pulping method |
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