CN1027316C - Human blood copper zinc superoxide dismutase color immunoassay plate - Google Patents
Human blood copper zinc superoxide dismutase color immunoassay plate Download PDFInfo
- Publication number
- CN1027316C CN1027316C CN 87101427 CN87101427A CN1027316C CN 1027316 C CN1027316 C CN 1027316C CN 87101427 CN87101427 CN 87101427 CN 87101427 A CN87101427 A CN 87101427A CN 1027316 C CN1027316 C CN 1027316C
- Authority
- CN
- China
- Prior art keywords
- sod
- color
- plate
- human blood
- blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 13
- 239000008280 blood Substances 0.000 title claims abstract description 13
- 238000003018 immunoassay Methods 0.000 title abstract 2
- TUGDLVFMIQZYPA-UHFFFAOYSA-N tetracopper;tetrazinc Chemical compound [Cu+2].[Cu+2].[Cu+2].[Cu+2].[Zn+2].[Zn+2].[Zn+2].[Zn+2] TUGDLVFMIQZYPA-UHFFFAOYSA-N 0.000 title abstract 2
- 238000000034 method Methods 0.000 claims abstract description 9
- 230000007935 neutral effect Effects 0.000 claims abstract description 4
- 150000003839 salts Chemical class 0.000 claims abstract description 3
- 238000006243 chemical reaction Methods 0.000 claims abstract 2
- 230000003053 immunization Effects 0.000 claims description 5
- 238000002649 immunization Methods 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 239000000975 dye Substances 0.000 claims description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 3
- 206010018910 Haemolysis Diseases 0.000 claims description 2
- 239000000427 antigen Substances 0.000 claims description 2
- 102000036639 antigens Human genes 0.000 claims description 2
- 108091007433 antigens Proteins 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 230000008588 hemolysis Effects 0.000 claims description 2
- 238000005185 salting out Methods 0.000 claims description 2
- 238000010186 staining Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 abstract description 9
- 239000010949 copper Substances 0.000 abstract description 9
- 210000002966 serum Anatomy 0.000 abstract description 3
- 238000003556 assay Methods 0.000 abstract description 2
- 238000007796 conventional method Methods 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 abstract description 2
- 229920000936 Agarose Polymers 0.000 abstract 1
- 102000001554 Hemoglobins Human genes 0.000 abstract 1
- 108010054147 Hemoglobins Proteins 0.000 abstract 1
- 238000010241 blood sampling Methods 0.000 abstract 1
- 210000003743 erythrocyte Anatomy 0.000 abstract 1
- 239000011546 protein dye Substances 0.000 abstract 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 3
- 108010012715 Superoxide dismutase Proteins 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 102000008221 Superoxide Dismutase-1 Human genes 0.000 description 1
- 108010021188 Superoxide Dismutase-1 Proteins 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229940125717 barbiturate Drugs 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 210000000624 ear auricle Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- -1 salt sodium chloride Chemical class 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229940032362 superoxide dismutase Drugs 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A color immunoassay plate for human blood copper zinc superoxide dismutase is a special assay plate for determining the total content of Cu and Zn-SOD in human blood erythrocytes. The plate is made of 1% ionic agarose containing protein dye, indicator, neutral salt and high-valence anti-human Cu, Zn-SOD serum. The interference of hemoglobin in the blood sample to the measurement is eliminated, and the complicated steps of the conventional method are omitted. The method has the advantages of less blood sampling amount, clear reaction result, easy observation and simple and convenient method, and is suitable for various medical and scientific research institutions.
Description
The present invention is the colored immune plate that is used for measuring human red cell copper, zinc one superoxide dismutase (CuproZinc-Superoxide Dismutase, Cu, Zn-SOD) content.
The method of measuring Cu, Zn-SOD at present has dianisidine method, chemoluminescence method etc., all is to utilize SOD dismutating superoxide radical anion (O
- 2) generate hydrogen peroxide and this principle of oxygen, measuring indirectly has active part SOD content, can not measure the SOD total content.And with determination of immunological methods SOD total content, mensuration program complexity, long flow path is only limited to the laboratory to use, and promotes difficulty.
In order to address this problem, the inspection means that SOD is measured as diagnosis and auxiliary diagnosis are applied to clinical hospital, make the colored immune plate that sets off the precipitation ring position with indicator, but because blood sample is hemolysate and non-leuco serum, the problem of haemoglobin interference measurement fails to eliminate.
The object of the present invention is to provide a kind of human blood SOD colour immunization test board.It can easy, rapid and precise mensuration human blood Cu, Zn-SOD content, eliminated the interference of haemoglobin to measuring, omitted loaded down with trivial details steps such as conventional method is embathed, dyeing, decolouring.
The present invention implements like this: add some protein dyestuff in recording the solution of colored immune plate, as Ponceaux, amino black etc., it can make antigen in the colored immune plate, antibody by specific staining, after diffusion-bonded becomes antigen-antibody complex, form the precipitation ring that color is deeper than colored immune plate background color; Also added certain density neutral salt, precipitation ring is revealed by salting out, made this plate color soft, the result is clear, observes clear.
The embodiment of the invention, the making of the colored immune plate of SOD: (ionic strength 0.025 in 1% agarose-barbiturate acid buffer, pH8.2) add amino black dye liquor, indicator phenolphthalein, antiseptic Sodium azide and neutral salt sodium chloride, after the heating for dissolving, add height tire anti-people Cu, Zn-SOD serum again, be layered on 5 * 10 centimetres the tailormadepiston plate cooled and solidified.Three horizontal rows are arranged to scale on this plastic plate, press mark punching according to the plate high scale, every horizontally-arranged 6 holes, totally 18 holes, 3 millimeters of every bore dias, place 4 ℃ stand-by.
Assay method is got people's ear-lobe blood 20 microlitres, adds in the 40 microlitre distilled water, makes its abundant haemolysis make hemolysate, place 4 ℃ to be measured.During mensuration, in measuring the hole, add standard SOD solution and each 10 microlitre of hemolysate to be measured respectively, this plate was at room temperature placed 15 hours, can directly read deposit ring diameter, obtain SOD content, represent with SOD microgram/every gram haemoglobin by calculating.
Owing to this people's blood superoxide dismutase colour immunization test board is provided, makes the mensuration of Cu, Zn-SOD can be applied to clinical examination.Only use micro-ear blood, just can measure SOD content quickly and accurately.Compare with other method, this method is easy, without special reagent and instrument, is not subjected to that other material disturbs in the blood, is easy to vast medical treatment, and R﹠D institution promotes the use of, particularly the relatively poor grass-roots unit of condition or use when generally investigating in a large number more convenient.
Claims (2)
1, a kind of antigen-antibody reaction mensuration human blood hepatocuprein that utilizes, the colour immunization test board of total content, it is characterized in that in making the solution of colored immune plate, adding the amino black dyestuff and make proteantigen in the colored immune plate, antibody, after diffusion-bonded becomes antigen antibody complex, form the precipitation ring that color is deeper than background color by specific staining; The neutral salt that has also added debita spissitudo makes precipitation ring come out by salting out, makes this plate color soft, and the result is clear.
2, a kind of method of using the described colour immunization test board of claim 1, it is characterized in that taking micro-ear blood, add to abundant haemolysis in the distilled water, make and place 4 ℃ of hemolysates to be measured and each 10 microlitre of standard SOD solution, insert respectively and measure in the hole, at room temperature placed 15 hours, and directly read deposit ring diameter, obtain the Cu.Zn-SOD total content as calculated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87101427 CN1027316C (en) | 1987-11-21 | 1987-11-21 | Human blood copper zinc superoxide dismutase color immunoassay plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87101427 CN1027316C (en) | 1987-11-21 | 1987-11-21 | Human blood copper zinc superoxide dismutase color immunoassay plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1039657A CN1039657A (en) | 1990-02-14 |
| CN1027316C true CN1027316C (en) | 1995-01-04 |
Family
ID=4813416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 87101427 Expired - Fee Related CN1027316C (en) | 1987-11-21 | 1987-11-21 | Human blood copper zinc superoxide dismutase color immunoassay plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1027316C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1037712C (en) * | 1995-05-29 | 1998-03-11 | 朱建安 | Method for measuring Zn content in urine |
-
1987
- 1987-11-21 CN CN 87101427 patent/CN1027316C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1039657A (en) | 1990-02-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Clegg et al. | Estimation of haemoglobin by the alkaline haematin method | |
| Brewer et al. | Methaemoglobin reduction test: a new, simple, in vitro test for identifying primaquine-sensitivity | |
| Serjeant et al. | A whole blood solubility and centrifugation test for sickle cell hemoglobin: a clinical trial | |
| EP0425945B1 (en) | Test device and method of assaying for fructoasmines | |
| US3733179A (en) | Method and apparatus for the quantitative determination of blood chemicals in blood derivatives | |
| CN106198527B (en) | A kind of ascorbic acid interference multi-term urine analysis test paper and preparation method thereof | |
| JPH0273134A (en) | Composition and method for testing aqueous solution in relation to specific gravity | |
| Stewart | Evaluation of a reagent-strip method for glucose in whole blood, as compared with a hexokinase method. | |
| CN1027316C (en) | Human blood copper zinc superoxide dismutase color immunoassay plate | |
| CN112198123B (en) | Detection system and detection method for correcting influence of anemia on glycated hemoglobin measurement value | |
| Allen | The significance of meconium in midtrimester genetic amniocentesis | |
| CN106290346A (en) | Mensuration reagent of superoxide dismutase and preparation method thereof | |
| CN108593633A (en) | A kind of Test paper for quickly detecting saliva uric acid | |
| CN111060503B (en) | G-6-PD detection kit free from anemia and detection method thereof | |
| CN212111450U (en) | Hemoglobin rapid detection reagent strip, kit and detection analyzer | |
| CN105973888B (en) | The kit of ischemia modified albumin IMA in a kind of quick detection blood | |
| Baron | Serum transaminases and isocitric dehydrogenase in kwashiorkor | |
| RU2018844C1 (en) | Method for diagnostics anemia at children | |
| JPS5845562A (en) | Method of measuring beta-lipoprotein fraction in body fluid and measurement reagent | |
| Ente et al. | Evaluation of a direct-reading reflectometer for neonatal hypoglycemia screening | |
| Matusik et al. | Rapid solubility test for detection of hemoglobin S | |
| Luzzatto et al. | The osmotic fragility of red cells in newborns and infants | |
| CN213843284U (en) | Myocardial infarction joint inspection test paper convenient to take | |
| Sophianopoulos et al. | Two new antisickling agents and the use of amino acid salts | |
| RU2050004C1 (en) | Method for determining membrane-bound erythrocyte catalase level |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |