CN102626385A

CN102626385A - Method for adding antibiotics into poultry oil emulsion inactivated vaccine

Info

Publication number: CN102626385A
Application number: CN2012101255916A
Authority: CN
Inventors: 叶孟海; 许正金; 游莉英; 谢金华; 范宏泉
Original assignee: FUJIAN SUMPO FOOD GROUP Co Ltd
Current assignee: FUJIAN SUMPO FOOD GROUP Co Ltd
Priority date: 2012-04-26
Filing date: 2012-04-26
Publication date: 2012-08-08

Abstract

The invention discloses a method for adding antibiotics into a poultry oil emulsion inactivated vaccine, which belongs to a technology field fused by animal husbandry poultry vaccine and antibiotics. Virus is inactivated to prepare an antigen, and finally emulsified oil is added to prepare a vaccine. The vaccine ensures the immune effect, saves a lot of time and manpower taken by medicament injection, has a good effect, and ensures a high survival rate of poultry.

Description

Add antibiotic method in the poultry oil emulsion inactivated vaccine

Technical field

The invention discloses the antibiotic method of adding in a kind of poultry oil emulsion inactivated vaccine, belong to the technical field that animal husbandry poultry vaccine and antibiotic merge.

Background technology

In order to prevent the poultry virus disease, the injection inactivated vaccine has obtained the common recognition of industry in the home poultry raising process; When breeding process generation bacterial disease, antibiotic application also is very general, because the poultry farming scale; Every batch of number of animals raised is all very big; Medication under normal circumstances is all with drinking-water or spice mode, though can save great amount of time and the manpower that injection spends, effect is more far short of what is expected than the mode of injection; For no other reason than that injection will spend the plenty of time, manpower, therefore antibiotic being injected in the home poultry raising industry not by widespread usage.

Summary of the invention

The objective of the invention is in the poultry oil emulsion inactivated vaccine, add antibiotic to the problems referred to above.Oil-emulsion inactivated vaccine mainly is that virus is processed antigen through after the deactivation, adds the vaccine that oil emulsion is processed at last; In oil-emulsion inactivated vaccine, adding antibiotic is feasible on pharmacology, because antibiotic is only effective to antibacterial theoretically, virus is not killed and interference effect.

The technical scheme that the present invention adopted is: the first step: virus is separated and is gathered; Second step: virus connects embryo, collects allantoic fluid; The 3rd step: inactivation of virus, antigen are made; The 4th step: key of the present invention: antibiotic preparation: the former powder of required antibiotic is dissolved in distilled water, guarantees to contain in every 250ml vaccine antibiotic 0.25-1 gram; The 5th step: water preparation: get vaccine antigen liquid and add the antibiotic liquid for preparing and be made into 1000ml with normal saline, add the Tween8040ml mix homogeneously then, it is subsequent use to process water; The 6th step: oil phase preparation: get the import white oil and mix with 6% Span80, add 2% aluminium stearate again by 94%, heating for dissolving then, abundant mix homogeneously carried out 115 ℃ of autoclavings 30 minutes at last; The 7th step: first emulsifying: water intaking is 1 part of slow completely joining in 2 parts of oil phases mutually, fully mixes; The 8th step: final emulsifying: first emulsive mixed liquor was put into high speed colloid mill (10000 rev/mins) 3 minutes, process at last and add anti-oil emulsion vaccine; The 9th step: packing, packing.

The advantage of drug administration by injection is following:

1, can get into blood very soon, reach blood drug level fast, therapeutic effect is fast.

2, save medicine, dosage is accurate.

3, overcome in the water of drinking-water administration and lost efficacy, or the problem of absorption difference in the intestinal.

4, the most handy injection system of some drugs such as PCs medicine uses, the drinking-water poor.

When injection deactivation oil seepage, add antibiotic starting point: not only can play immunization, also have following effect:

1, can reduce the infection at vaccine injection position.

2, can block disease,, improve the early stage survival rate of baby chick like Salmonella etc. (according to investigations, it is higher that domestic kind of chicken infects the Salmonella ratio, and Sanhuang chicken infects more general) because of the seedling vertical transmission.

3, after the minimizing vaccine injection respiratory tract disease and secondary infection take place.

4, drug cost is practiced thrift in the drinking-water administration after the minimizing immunity.

5, vaccine injection time early stage in home poultry raising all adds antibiotic and can not cause the meat drug residue.

Description of drawings

Fig. 1 is the method process chart that adds antibiotic method in the poultry oil emulsion inactivated vaccine of the present invention.

The specific embodiment

Below in conjunction with accompanying drawing the specific embodiment of the invention is further specified: the first step: virus is separated and is gathered; Second step: virus connects embryo, collects allantoic fluid; The 3rd step: inactivation of virus, antigen are made; The 4th step: key of the present invention: antibiotic preparation: the former powder of required antibiotic is dissolved in distilled water, guarantees to contain in every 250ml vaccine antibiotic 0.25-1 gram; The 5th step: water preparation: get vaccine antigen liquid and add the antibiotic liquid for preparing and be made into 1000ml with normal saline, add the Tween8040ml mix homogeneously then, it is subsequent use to process water; The 6th step: oil phase preparation: get the import white oil and mix with 6% Span80, add 2% aluminium stearate again by 94%, heating for dissolving then, abundant mix homogeneously carried out 115 ℃ of autoclavings 30 minutes at last; The 7th step: first emulsifying: water intaking is 1 part of slow completely joining in 2 parts of oil phases mutually, fully mixes; The 8th step: final emulsifying: first emulsive mixed liquor was put into high speed colloid mill (10000 rev/mins) 3 minutes, process at last and add anti-oil emulsion vaccine; The 9th step: packing, packing.

Clinical test results: this test divides lab scale, pilot scale, tries three completion greatly, and three repeating groups are established in each test.EXPERIMENTAL DESIGN: the new tributary oil-emulsion inactivated vaccine of matched group oil-emulsion inactivated vaccine 0.3ml/ plumage, test group injection added with antibiotic in the white new tributary of plumage broiler 5 age in days cervical region subcutaneous injections, other is identical with matched group, and survival rate result is following:

Lab scale: every group of test chicken 1000 plumages

	Matched group	Test one group	Test two groups	Test three groups
					Injection back one all survival rate %	98.8	100.7	100.8	100.6
Injection back two all survival rate %	97.4	100.1	100.3	99.9
					Deliver survival rate % (42 days) for sale	94.3	98.2	98.1	97.7

Pilot scale: every group of test chicken 16500 plumages

	Matched group	Test one group	Test two groups	Test three groups
					Injection back one all survival rate %	99.16	101.24	100.92	100.96
Injection back two all survival rate %	97.88	100.49	100.33	100.32
					Deliver survival rate % (42 days) for sale	95.20	98.85	98.45	98.29

Big examination: every group of test chicken 37000 plumages

	Matched group	Test one group	Test two groups	Test three groups
					Injection back one all survival rate %	99.32	100.64	100.68	101.23

Injection back two all survival rate %	98.03	100.08	100.24	100.76
					Deliver survival rate % (42 days) for sale	95.85	98.56	98.36	98.58

Remarks: by the industry rule, advancing per 100 plumages of baby chick and give 2 plumages as loss, is 102% so advance Seedling survival rate on the same day.

Analyze: 1, from lab scale, can find out, test one, two, three group of vaccine injection after all average survivals higher by 1.9% than matched group, afterwards two all average survivals are higher by 2.7% than matched group in injection, it is higher by 3.7% than matched group to deliver survival rate for sale.

2, from pilot scale, can find out, test one, two, three group of vaccine injection after all average survivals higher by 1.55% than matched group, injection back two all average survivals are higher by 2.5% than matched group, it is higher by 3.33% than matched group to deliver survival rate for sale.

3, from big examination, can find out, test one, two, three group of vaccine injection after all average survivals higher by 1.53% than matched group, injection back two all average survivals are higher by 2.33% than matched group, it is higher by 2.65% than matched group to deliver survival rate for sale.

Sum up: injection adds anti-oil seepage can significantly reduce the baby chick death rate of injection back in two weeks, and effect is remarkable; Effect to raising middle and late stage is not remarkable.

Deliver chicken antibody for sale and detect (examining each 30 plumages altogether three times): ND (newcastle), the contrast of H9 (low pathogenicity bird flu) antibody:

For the first time:

For the second time:

For the third time:

Sum up: add anti-oil seepage when broiler is delivered for sale, test group antibody and matched group difference are not remarkable, explain to add antibiotic in the vaccine to not influence of vaccine immunity.

Above-described embodiment, the present invention specific embodiment a kind of more preferably just, common variation that those skilled in the art carries out in technical scheme scope of the present invention and replacement all should be included in protection scope of the present invention.

Claims

1. add antibiotic method in the poultry oil emulsion inactivated vaccine, it is characterized in that: the first step: virus is separated and is gathered; Second step: virus connects embryo, collects allantoic fluid; The 3rd step: inactivation of virus, antigen are made; The 4th step: antibiotic preparation: required antibiotic is dissolved in distilled water; The 5th step: water preparation: get vaccine antigen liquid and add the antibiotic liquid for preparing and use normal saline, add the Tween80 mix homogeneously then, it is subsequent use to process water; The 6th step: oil phase preparation: get import white oil Span80 and mix, add aluminium stearate again, heating for dissolving then, fully mix homogeneously carries out autoclaving at last; The 7th step: first emulsifying: water intaking is 1 part of slow completely joining in 2 parts of oil phases mutually, fully mixes; The 8th step: final emulsifying: first emulsive mixed liquor is put into the high speed colloid mill, process at last and add anti-oil emulsion vaccine; The 9th step: packing, packing.

2. according to claim 1, add antibiotic method in the poultry oil emulsion inactivated vaccine, it is characterized in that: the 4th step: the antibiotic preparation: the former powder of required antibiotic is dissolved in distilled water, guarantees to contain in every 250ml vaccine antibiotic 0.25-1 gram; The 5th step: water preparation: get vaccine antigen liquid and add the antibiotic liquid for preparing and be made into 1000ml with normal saline, add the Tween8040ml mix homogeneously then, it is subsequent use to process water; The 6th step: oil phase preparation: get the import white oil and mix with 6% Span80, add 2% aluminium stearate again by 94%, heating for dissolving then, abundant mix homogeneously carried out 115 ℃ of autoclavings 30 minutes at last; The 7th step: first emulsifying: water intaking is 1 part of slow completely joining in 2 parts of oil phases mutually, fully mixes; The 8th step: final emulsifying: first emulsive mixed liquor was put into high speed colloid mill (10000 rev/mins) 3 minutes, process at last and add anti-oil emulsion vaccine.