CN102618070A - Purification method of functional pigment in taraxacum flower - Google Patents

Abstract

The invention relates to a purification method of functional pigment in taraxacum flower, which comprises the following steps: drying taraxacum flower, crushing and screening by a screen of 80 meshes; mixing powder with distilled water, and extracting under the conditions of ultrasonic power being 100W and the temperature being 55 DEG C to obtain precipitate; adding 75% ethanol to the precipitate, extracting under the conditions of ultrasonic power being 100W and the temperature being 55 DEG C for three times, and combining filtrates; adding the filtrate to a rotary evaporator for evaporation, concentrating until ethanol smell disappears, and drying to obtain a pigment crude extract; immersing the extract in distilled water, adjusting the pH of the solution to 3.0 with hydrochloric acid, and adsorbing; collecting 55% and 75% ethanol eluates; and carrying out concentration by evaporation on the eluate in a rotary evaporator, and drying the concentrate at the temperature of 45 DEG C in vacuum to obtain brown yellow powder with purity more than 90%. The yellow pigment extracted by the method is pure in color, strong in coloring force, high in stability and high in contents of total flavones and total phenolic acids.

Description

Functional pigmented method of purification in the dandelion flower

Technical field

The present invention relates to a kind of method of purification of natural pigment, be specifically related to method of purification functional pigmented in a kind of dandelion flower.

Background technology

Food dye is very important foodstuff additive, and it is given food and pleases beautiful color and luster, gives beautiful enjoyment, and appetite stimulator is played an important role, and it also is the use pigment that industries such as makeup, medicine are praised highly in recent years simultaneously.Food dye is divided into synthetic colour and two big types of progress along with medical science toxicology, biology and analytical technology of natural pigment; People find that successively many kinds have serious chronic toxicity and carinogenicity in the edible chemosynthesis pigment, so the chemosynthesis pigment is substituted by natural pigment just gradually.And characteristics such as that edible natural pigment has is safe, color and luster nature, a lot of natural pigments contain the nutritive substance of needed by human, or itself are exactly VITAMINs or material with VITAMINs character.Also have some natural pigments to have certain pharmacological function, some disease is had prevention and therapeutic action.Play an important role at aspects such as removing radical, anti-oxidant, hypotensive, reducing blood-fat, adjusting immunizing power, liver protecting like a lot of natural pigments.

At present, pure natural yellow pigment proportion in industries such as food, medicine is merely 10%, and domestic every year is with 20%～30% demand speed increment; The heavy demand of foreign market in addition, only the demand of Japan just reaches 500t/a, and natural yellow pigment demonstrates the impetus that supply falls short of demand; Along with China's food, medical treatment, the growth of light industry; Also in continuous increase, but existing pigment far can not satisfy the needs of productive life to people to the kind of natural pigment and demand, only natural pigment at home market demand more than 100,000 tons; Account for 60% of pigment aggregate demand; And 6000 tons of China's yellow pigment actual output less thaies, a large amount of needs of foreign market in addition, price is expected to rise year by year.The information that provides according to foreign trade department, at least within nearly 5 years, yellow pigment market all can supply falls short of demand.

Taraxacum (Talraxacum mongolicum Hand.-Mass), all parts of the country are generally wild, and the dandelion flower florescence is long, and flower is big, and aboundresources is a kind of composite family per nnial herb, herb can be used as medicine, and is the heat-clearing and detoxifying herb of using always.Modern pharmacological research shows, effect such as that taraxacum has is antibiotic, anti-inflammatory, cholagogic, antitumor and immunomodulatory is extensive in clinical application.Also contain the nutritive ingredient of many needed by human in the dandelion flower, like biologically active substances such as amino acid, flavonoid and phenolic acids, of great advantage to promoting health.Also have the research report, extract has effects such as antibacterial, anti-inflammatory in the taraxacum.Therefore from natural taraxacum, seek existing colouring function, the natural food colour that has physiological functions such as medicinal and nutrition has again become the emphasis that various countries competitively research and develop.

Summary of the invention

Technical problem to be solved by this invention provides method of purification functional pigmented in a kind of dandelion flower, and the yellow pigment color and luster that this method is extracted is pure, strong coloring force, and stability is high, and total flavones and total phenolic content are high in the yellow pigment.

Technical scheme of the present invention: functional pigmented method of purification in a kind of dandelion flower, this method may further comprise the steps:

(1) get fresh dandelion flower and remove holder, through dry, pulverizing, it is subsequent use to cross 80 mesh sieves;

(2) subsequent use powder being mixed with the solid-liquid ratio of zero(ppm) water by 1: 20, is that 100W, temperature are to extract 3 times under 55 ℃ the condition at ultrasonic power, and each 30min filters, and discards filtrating, throw out, subsequent use;

(3) in throw out, adding concentration is 75% ethanol, is that 100W, temperature are to extract 3 times under 55 ℃ the condition at ultrasonic power, filters, and merges 3 times filtrating, subsequent use;

The filtrating that (4) will make adds in the rotatory evaporator, in temperature is to evaporate under 45 ℃ the condition, is concentrated into no ethanol and distinguishes the flavor of, and cryodrying gets the pigment crude extract;

(5) the pigment crude extract that makes is immersed in the zero(ppm) water, and with hydrochloric acid conditioning solution to PH=3.0, and solution made an addition to the capital in the resin bed of packing into, adsorb with the flow velocity of 2.0BV/h;

(6) getting flow respectively is 4BV, and to be 25%, 55%, 75% ethanolic soln carry out wash-out with the flow velocity of 2.0BV/h to concentration successively, and discarding concentration is 25% ethanol eluate, and collecting concentration is 55% and 75% ethanol eluate;

(7) with above-mentioned elutriant in rotatory evaporator, be evaporation concentration under 55 ℃ of conditions in temperature, again with enriched material in temperature be under 45 ℃ the vacuum condition dry purity greater than 90% pale brown toner end.

The present invention compared with prior art has following beneficial effect: 1, pure, the nature of yellow pigment color and luster in the dandelion flower of this method extraction; Strong coloring force, stability is high, and total flavones and total phenolic content are higher in the yellow pigment; Have certain oxidation-resistance and biocidal property; Be a kind of safe natural pigment, simultaneously it also can make natural antioxidants, functional pigmentedly in numerous food, uses as a kind of.

2, pigment is dissolved ethanol after, solution color and luster cadmium yellow, the staple flavonoid compound of this pigment all has stronger removing effect to radical and grease autoxidation, and human body is had certain function of health care.

3, taraxacum aboundresources is processed product with the product that this method is extracted, and puts on market, can satisfy the demand of existing market, this method apply society and economic benefit considerable, have vast market prospect.

Embodiment

Functional pigmented method of purification in a kind of dandelion flower, this method may further comprise the steps:

(1) get fresh dandelion flower and remove holder, through dry, pulverizing, it is subsequent use to cross 80 mesh sieves;

(2) getting subsequent use powder 1.0g and mix with the solid-liquid ratio of 20ml zero(ppm) water by 1: 20, is that 100W, temperature are to extract 3 times under 55 ℃ the condition at ultrasonic power, and each 30min filters, and discards filtrating, throw out, subsequent use;

(3) in throw out, adding 100ml concentration is 75% ethanol, is that 100W, temperature are to extract 3 times under 55 ℃ the condition at ultrasonic power, filters, and merges 3 times filtrating, and common 300ml is subsequent use;

(4) 300ml that makes filtrating being added in the rotatory evaporator, is to evaporate under 45 ℃ the condition in temperature, is concentrated into no ethanol and distinguishes the flavor of, and cryodrying gets pigment crude extract 0.23g;

(5) the pigment crude extract that makes is immersed in the zero(ppm) water of 60ml, and with hydrochloric acid conditioning solution to PH=3.0, and solution made an addition to the capital of 1.5cm * 20cm in the D-101 type resin bed of packing into, adsorb with the flow velocity of 2.0BV/h;

(6) getting flow respectively is 4BV, and to be 25%, 55%, 75% ethanolic soln carry out wash-out with the flow velocity of 2.0BV/h to concentration successively, and discarding concentration is 25% ethanol eluate, and collecting concentration is 55% and 75% ethanol eluate, altogether 280ml;

(7) with above-mentioned elutriant in rotatory evaporator, be that evaporation concentration is dry under 45 ℃ the vacuum condition in temperature with enriched material to 10ml again under 55 ℃ of conditions in temperature, purity greater than 90% pale brown toner end 0.13g, productive rate is 13%.

Claims (1)

1. functional pigmented method of purification in the dandelion flower is characterized in that this method may further comprise the steps:

(1) get fresh dandelion flower and remove holder, through dry, pulverizing, it is subsequent use to cross 80 mesh sieves;

(2) subsequent use powder being mixed with the solid-liquid ratio of zero(ppm) water by 1: 20, is that 100W, temperature are to extract 3 times under 55 ℃ the condition at ultrasonic power, and each 30min filters, and discards filtrating, throw out, subsequent use;

(3) in throw out, adding concentration is 75% ethanol, is that 100W, temperature are to extract 3 times under 55 ℃ the condition at ultrasonic power, filters, and merges 3 times filtrating, subsequent use;

The filtrating that (4) will make adds in the rotatory evaporator, in temperature is to evaporate under 45 ℃ the condition, is concentrated into no ethanol and distinguishes the flavor of, and cryodrying gets the pigment crude extract;

(5) the pigment crude extract that makes is immersed in the zero(ppm) water, and with hydrochloric acid conditioning solution to PH=3.0, and solution made an addition to the capital in the resin bed of packing into, adsorb with the flow velocity of 2.0BV/h;

(6) getting flow respectively is 4BV, and to be 25%, 55%, 75% ethanolic soln carry out wash-out with the flow velocity of 2.0BV/h to concentration successively, and discarding concentration is 25% ethanol eluate, and collecting concentration is 55% and 75% ethanol eluate;

(7) with above-mentioned elutriant in rotatory evaporator, be evaporation concentration under 55 ℃ of conditions in temperature, again with enriched material in temperature be under 45 ℃ the vacuum condition dry purity greater than 90% pale brown toner end.