CN102559371B - Method for preparing phosphatidylserine-enriched krill oil - Google Patents
Method for preparing phosphatidylserine-enriched krill oil Download PDFInfo
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- CN102559371B CN102559371B CN 201210038744 CN201210038744A CN102559371B CN 102559371 B CN102559371 B CN 102559371B CN 201210038744 CN201210038744 CN 201210038744 CN 201210038744 A CN201210038744 A CN 201210038744A CN 102559371 B CN102559371 B CN 102559371B
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Abstract
The invention provides a method for preparing phosphatidylserine-enriched krill oil. The method comprises the following steps of: a) unfreezing frozen Neptune krill, adding each 1kg of Neptune krill into 1.5 to 2.5L of 90 to 100 weight percent ethanol solution, extracting for 2 to 4 times with stirring, extracting for 6 to 8 hours each time, mixing extracting solutions, and filtering to obtain filtrate; b) concentrating the filtrate obtained in the step a) at the temperature of between 60 to 65DEG C and the pressure of between -0.07 and -0.09MPa until the filtrate is 11 to 12 volume percent of the mixed filtrate, thus obtaining a concentrated extracting solution; c) adding n-hexane in the same volume as the concentrated extracting solution into the concentrated extracting solution obtained in the step b), uniformly mixing, standing for demixing, and taking the second layer of liquid from top to bottom; and d) evaporating the liquid taken in the step c) at the temperature of between 50and 55DEG C and the pressure of between -0.07 and -0.09MPa to remove the n-hexane to obtain the product.
Description
Technical field
The present invention relates to medicine, chemical, food, health food technology field, further say to relate to a kind of method that from krill, prepares the shrimp sauce that is rich in phosphatidylserine.
Background technology
Krill is lived in the South Pole fresh water of Antarctic Ocean, lives in the mode of trooping.Nearly 500,000,000 tons, annual approximately 100,000 tons of now whole world krill fishery is the abundant resource overseas that China is about to exploitation in recent years.Freezing krill general moisture about 80%, phospholipids content is higher in the krill grease, main component in the phosphatide is phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphinylidyne acyl inositol (PI), phosphatidic acid (PA) and phosphatidylserine (PS).According to the data record, the phosphatidylserine in the antarctic krill phospholipid (PS) content is very low, accounts for 2% of total phospholipids.Studies show that PS is the same with other phospholipid compositions has emulsification, wetting action and a unique effect that other phospholipid compositions did not have.
The main raw materials of phosphatidylserine is the ox brain, or synthetic under the catalysis of Phospholipid hydrolase with soybean phospholipid and Serine.The medical effect of ox kephalin acyl Serine is very obvious, and the synthetic phosphatidylserine of soybean phospholipid and Serine does not obtain producing always and implements.Phosphatidylserine has a lot of physiological functions, as affects cell membrane fluidity, raising brain memory, alleviation senile dementia etc., and its medical function mainly comprises: 1. on the impact of animal brain and learning process, can improve learning behavior and memory; 2. on the impact of maincenter bioelectrical activity; 3. in the clinical application, can slow down some biological variation of Animal Aging, the cognition dysfunction that especially aging is caused has certain curative effect.So far, also for seeing the report that extracts phosphatidylserine from krill is arranged.
Summary of the invention
The objective of the invention is according to the existing market needs, provide a kind of easy and simple to handle, the method for the shrimp sauce of phosphatidylserine is rich in the simple preparation of technological process.
Purpose of the present invention can realize by following technical measures:
The method is carried out as follows:
A. getting freezing krill thaws, then be that the proportioning of 90～100% ethanols joins krill in the ethanol according to 1 kilogram of krill and 1.5～2.5 liter weight concentration, stir and extract each 6 ~ 8h 2～4 times down, united extraction liquid also filters, and gets filtrate;
B. with the filtrate of a step in 60～65 ℃ ,-0.07～-be concentrated into 11～12% of merging filtrate volume under the 0.09MPa condition, get concentrated extracting solution;
C, add the normal hexane with volume in the concentrated extracting solution of b step, mixing, quiet putting just can be told well-defined four layers immediately, then from up to down gets the 2nd layer of liquid;
D, liquid that the c step is taken out in 50～55 ℃ and-0.07～-the 0.09MPa pressure condition under evaporation remove normal hexane, get faint yellow shrimp sauce product.Phospholipids content is at 8-10% in measuring shrimp sauce.And PS accounts for more than 40% of phosphatide.
The deep processing of krill grease is the problem that must face future, contain a small amount of phosphatidylserine (PS) in the krill grease, and this kind PS has good value medical health care, but from krill, extract at present the preparation phosphatidylserine and yet there are no report, in view of phosphatidylserine in the krill good value medical health care and current demand to the krill deep process technology, the present invention adopts solvent-extraction process to prepare the shrimp sauce that is rich in phosphatidylserine from krill, more general solvent extraction process is easy and simple to handle, technological process is simple, that separates is rich in the phosphatidylserine liquid level phospholipids content more than 8%, reach as high as 10%, wherein phosphatidylserine accounts for more than 40%.The senior krill oil that is rich in PS of this invention preparation can be used as soft capsule and directly uses, also can be on this basis as the raw material that continues purification.
Embodiment
Embodiment 1:
A. getting freezing krill and thaw, is that the proportioning of 90% ethanol joins krill in the ethanol according to 500g krill and 750mL weight concentration then, stirs lower the extraction 4 times, each 6h, and united extraction liquid also filters, and gets filtrate;
B. with the filtrate of a step in 60 ℃, be concentrated into 11% of merging filtrate volume under the-0.09MPa condition, get concentrated extracting solution;
C. add the normal hexane with volume in the concentrated extracting solution of b step, mixing, quiet putting told well-defined four layers immediately, then from up to down gets the 2nd layer of liquid;
D, liquid that the c step is taken out in 55 ℃ and-the 0.07MPa pressure condition under evaporation remove normal hexane, get faint yellow shrimp sauce, shrimp sauce contains phosphatide 8%, wherein PS accounts for 41% of phosphatide.
Embodiment 2:
A. getting freezing krill and thaw, is that the proportioning of 100% ethanol joins krill in the ethanol according to 500g krill and 1250mL weight concentration then, stirs lower the extraction 2 times, each 8h, and united extraction liquid also filters, and gets filtrate;
B. with the filtrate of a step in 65 ℃, be concentrated into 12% of merging filtrate volume under the-0.07MPa condition, get concentrated extracting solution;
C. add the normal hexane with volume in the concentrated extracting solution of b step, mixing, quiet putting told well-defined four layers immediately, then from up to down gets the 2nd layer of liquid;
D, liquid that the c step is taken out in 50 ℃ and-the 0.09MPa pressure condition under evaporation remove normal hexane, get faint yellow shrimp sauce, shrimp sauce contains phosphatide 10%, wherein PS accounts for 40% of phosphatide.
Embodiment 3:
A. getting freezing krill and thaw, is that the proportioning of 95% ethanol joins krill in the ethanol according to 500g krill and 1000mL weight concentration then, stirs lower the extraction 3 times, each 7h, and united extraction liquid also filters, and gets filtrate;
B. with the filtrate of a step in 63 ℃, be concentrated into 11.5% of merging filtrate volume under the-0.08MPa condition, get concentrated extracting solution;
C. add the normal hexane with volume in the concentrated extracting solution of b step, mixing, quiet putting told well-defined four layers immediately, then from up to down gets the 2nd layer of liquid;
D, liquid that the c step is taken out in 52 ℃ and-the 0.08MPa pressure condition under evaporation remove normal hexane, get faint yellow shrimp sauce, shrimp sauce contains phosphatide 9%, wherein PS accounts for 40.05% of phosphatide.
Claims (1)
1. method for preparing the shrimp sauce that is rich in phosphatidylserine is characterized in that the method carries out as follows:
A. getting freezing krill and thaw, is that the proportioning of 95% ethanol joins krill in the ethanol according to 500g krill and 1000mL weight concentration then, stirs lower the extraction 3 times, each 7h, and united extraction liquid also filters, and gets filtrate;
B. with the filtrate of a step in 63 ℃, be concentrated into 11.5% of merging filtrate volume under the-0.08MPa condition, get concentrated extracting solution;
C, add the normal hexane with volume in the concentrated extracting solution of b step, mixing, quiet putting told well-defined four layers immediately, then from up to down gets the 2nd layer of liquid;
D, liquid that the c step is taken out in 52 ℃ and-the 0.08MPa pressure condition under evaporation remove normal hexane, get product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201210038744 CN102559371B (en) | 2012-02-21 | 2012-02-21 | Method for preparing phosphatidylserine-enriched krill oil |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210038744 CN102559371B (en) | 2012-02-21 | 2012-02-21 | Method for preparing phosphatidylserine-enriched krill oil |
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| CN102559371A CN102559371A (en) | 2012-07-11 |
| CN102559371B true CN102559371B (en) | 2013-09-18 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1516592A (en) * | 2001-06-18 | 2004-07-28 | �����Ǽ���&������Դ����˾ | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases, arthritis, skin cancers, diabetes, premenstrual syndrome and transdermal transport |
| CN1583766A (en) * | 2004-06-01 | 2005-02-23 | 山东师范大学 | Extraction of phosphatidyl serine from brains of animals |
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2012
- 2012-02-21 CN CN 201210038744 patent/CN102559371B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1516592A (en) * | 2001-06-18 | 2004-07-28 | �����Ǽ���&������Դ����˾ | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases, arthritis, skin cancers, diabetes, premenstrual syndrome and transdermal transport |
| CN1583766A (en) * | 2004-06-01 | 2005-02-23 | 山东师范大学 | Extraction of phosphatidyl serine from brains of animals |
Non-Patent Citations (10)
| Title |
|---|
| Extraction and characterisation of lipids from Antarctic Krill(Euphausia superba);Joseph C.Gigliotti et al;《Food Chemistry》;20111231;1028-1036 * |
| Joseph C.Gigliotti et al.Extraction and characterisation of lipids from Antarctic Krill(Euphausia superba).《Food Chemistry》.2011, |
| 南极大磷虾油脂的提取及其脂肪酸组成分析;赵传凯 等;《食品工业科技》;20120215;第33卷(第3期);207-209 * |
| 南极磷虾粗虾油提取工艺优化;崔秀明 等;《食品科学》;20111231;第32卷(第24期);126-129 * |
| 南美白对虾虾头中磷脂提取工艺的优化;边晶晶 等;《食品科学》;20111231;第32卷(第24期);11-15 * |
| 崔秀明 等.南极磷虾粗虾油提取工艺优化.《食品科学》.2011,第32卷(第24期), |
| 浸提法提取虾油脂的最佳条件组合;陈艳 等;《湖北农业科学》;20090131;第48卷(第1期);181-183 * |
| 赵传凯 等.南极大磷虾油脂的提取及其脂肪酸组成分析.《食品工业科技》.2012,第33卷(第3期), |
| 边晶晶 等.南美白对虾虾头中磷脂提取工艺的优化.《食品科学》.2011,第32卷(第24期), |
| 陈艳 等.浸提法提取虾油脂的最佳条件组合.《湖北农业科学》.2009,第48卷(第1期), |
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