CN102507553A - Rapid detection kit of nitrite nitrogen in aquiculture water and detection method using same - Google Patents
Rapid detection kit of nitrite nitrogen in aquiculture water and detection method using same Download PDFInfo
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- CN102507553A CN102507553A CN2011103282985A CN201110328298A CN102507553A CN 102507553 A CN102507553 A CN 102507553A CN 2011103282985 A CN2011103282985 A CN 2011103282985A CN 201110328298 A CN201110328298 A CN 201110328298A CN 102507553 A CN102507553 A CN 102507553A
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Abstract
The invention relates to a rapid detection kit of nitrite nitrogen in aquiculture water. The kit internally comprises one 10 mL stoppered cuvette, one colorimetric card, one suction pipe, two 20 mL dropping bottles, 20 mL of reagent A and 20 mL of reagent B. The detection method using the kit comprises the following specific operation steps of: (1) washing the cuvette 2-3 times with the water to be detected; (2) sampling 5 mL of water sample, adding 5 drops of the reagent A, then adding 5 drops of reagent B and uniformly shaking; and (3) covering the cuvette, lightly shaking, standing for 1-3 minutes, closing the cuvette to the blank of the colorimetric card, and comparing the color of the liquid in the cuvette with the color of the colorimetric card to obtain the content (mg/L) of nitrite nitrogen in the detected water sample. According to the invention, the content of nitrite nitrogen in the water sample to be detected is displayed by a developing agent, thus the rapid detection kit has the characteristics of being accurate, rapid, convenient, inexpensive and the like, does not need for any equipment, and adopts a counting drop method to measure; and is used for field rapid detection of water quality of aquiculture water, domestic sewage, industrial wastewater and the like, the rapid detection kit is very simple in use method.
Description
Technical field
The present invention relates to a kind of chemical constitution detection kit and detection method thereof, particularly a kind of aquaculture water nitrite nitrogen quick detection kit and detection method thereof.
Background technology
In the aquatic animal breeding process, especially under the high-density breeding pattern, nitrite is one of main factor of restriction aquatic animal normal growth; This is owing to the increase along with culturing time; Cultivation water nitrite can accumulate gradually, when its concentration reaches certain value, not only can produce directly fish and poison; And can bring out multiple disease, thereby have influence on culture benefit.
After the nitrite poisoning, the oxygen carrying capacity of blood weakens, and cultures object than the symptom that is easier to form similar anoxic.When the nitrite slow poisoning took place aquatic animal, unclear symptoms showed, and naked eyes are difficult to find out, have a strong impact on the growth of aquatic products; The food ration of poisoning darker reduces, hypoergia, and the fish body is become thin, and body surface is matt, causes death in enormous quantities at last.
Along with the continuous appearance and the development of analytical chemistry new method and new technology, the diversification more of the detection method of nitrite mainly contains photometry, oscilloscopic polarography, fluorescence method, the chromatography of ions etc.All relate in the said method and use large-scale instrument and equipment and reagent.Therefore; Need based on the clinical handling characteristics of current aquaculture; Needing badly provides a kind of convenient, fast and detection method that susceptibility is high; Promptly developing a kind of aquaculture water nitrite nitrogen quick detection kit and detection method thereof, benefit culture fishery, will be that this technical field scientific research personnel is badly in need of one of new problem of furtheing investigate exploitation.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, the significant aquaculture water of a kind of effect nitrite nitrogen quick detection kit and detection method thereof are provided.
Following for realizing the embodiment that above-mentioned purpose the present invention adopted:
A kind of aquaculture water nitrite nitrogen quick detection kit is characterized in that being provided with in this kit:
10ml tool plug cuvette: 1;
Colorimetric card: 1;
Suction pipe: 1;
20ml drop bottle: 2;
Reagent A: 20ml;
Reagent B:20ml;
The preparation of reagent A (500ml): measure 50ml phosphoric acid in the 200ml beaker, phosphoric acid concentration is 15mol/L=1.70g/mL, adds deionized water 200ml it is mixed; Take by weighing 20g 4-aminobenzene sulfonamide and put into said beaker, make its dissolving after, be transferred in the 500ml volumetric flask, be settled to 500ml with deionized water, packing, the 20ml/ bottle keeps in Dark Place;
The preparation of reagent B (500ml): take by weighing 1-naphthyl ethylenediamine dihydrochloride 1g and be dissolved in the 300ml deionized water, it is transferred in the 500ml volumetric flask is settled to 500ml with deionized water, packing, the 20ml/ bottle keeps in Dark Place.
The concrete operations step of this kit test method is following:
(1) washes cuvette 2～3 times with water to be checked;
(2) water sampling 5ml; Add 5 reagent A, add 5 reagent B again and shake up;
(3) build cuvette and also shake up gently, placed 1～3 minute; Cuvette is affixed on the blank space of colorimetric card, with the colorimetric card color relatively get final product the nitrite nitrogen content (mg/L) of the water sample of surveying.
The detection principle of kit of the present invention: nitrite generates diazo salt with sulfanilic acid generation diazo-reaction under acid condition, this diazo salt with alpha-naphthylamine reagent generation coupling reaction, generate the aubergine azo-compound.The degree of depth of its color is directly proportional with appearance liquid Central Asia nitric acid content, but colorimetric estimation.This nitrite nitrogen quick detection kit sensing range is 0.005～0.3mg/L.
The invention has the beneficial effects as follows: kit of the present invention is to utilize developer to show nitrite nitrogen content in the water sample to be measured, has characteristics such as accurate, quick, convenient, inexpensive, without any need for equipment, adopts several methods to measure; Can be used for aquaculture water, Drinking Water and source water thereof; Surface water, seawater; The field quick detection of multiple water quality such as sanitary sewage and industrial waste water is widely used; Reagent in the box provides with the form of working fluid, and method of application is very simple, measures reliable results; Volume is little, and is in light weight, easy to carry; Can accomplish the analytical work of a water sample in 3～5 minutes; The check and analysis expense is low; The tester need not training, only needs the by specification procedure to get final product.
Main agents in nitrite nitrogen quick detection kit provided by the present invention and the detection method thereof provides with the working fluid form, and is easy to use, and reagent is easy to preserve.Nitrite generates diazo salt with sulfanilic acid generation diazo-reaction under acid condition; This diazo salt with alpha-naphthylamine reagent generation coupling reaction; Generate the aubergine azo-compound; Its colourity is directly proportional with the nitrite nitrogen content, adopts visual colorimetry, and concentration limit is 0.005mg/L.This kit can supply 80 detections to use, and effectively reduces and detects cost.Therefore, the present invention efficiently solves the demand of fast detecting water quality nitrite nitrogen in the present aquaculture, has solved to use large-scale detector to waste time and energy and be not suitable for a difficult problem such as on-site measurement.This kit has broad application prospects in the water quality detection field, and has huge economic benefit and social benefit.
Embodiment
Below in conjunction with preferred embodiment, to details are as follows according to embodiment provided by the invention:
Embodiment 1
A kind of aquaculture water nitrite nitrogen quick detection kit is characterized in that being provided with in this kit:
10ml tool plug cuvette: 1;
Colorimetric card: 1; (adopting existing colorimetric card)
Suction pipe: 1;
20ml drop bottle: 2;
Reagent A: 20ml;
Reagent B:20ml;
The preparation of reagent A (500ml): measure 50ml phosphoric acid in the 200ml beaker, phosphoric acid concentration is 15mol/L=1.70g/mL, adds deionized water 200ml it is mixed; Take by weighing 20g 4-aminobenzene sulfonamide and put into said beaker, make its dissolving after, be transferred in the 500ml volumetric flask, be settled to 500ml with deionized water, packing, the 20ml/ bottle keeps in Dark Place;
The preparation of reagent B (500ml): take by weighing 1-naphthyl ethylenediamine dihydrochloride 1g and be dissolved in the 300ml deionized water, it is transferred in the 500ml volumetric flask is settled to 500ml with deionized water, packing, the 20ml/ bottle keeps in Dark Place.
The concrete operations step of this kit test method is following:
(1) washes cuvette 2～3 times with water to be checked;
(2) water sampling 5ml; Add 5 reagent A with suction pipe, add 5 reagent B again and shake up;
(3) build cuvette and also shake up gently, placed 1～3 minute; Cuvette is affixed on the blank space of colorimetric card, with the colorimetric card color relatively get final product the nitrite nitrogen content (mg/L) of the water sample of surveying.
This nitrite nitrogen quick detection kit sensing range is 0.005～0.3mg/L, is used for Drinking Water and source water thereof; Surface water, aquaculture water, seawater; The field quick detection of multiple water quality such as sanitary sewage and industrial waste water.
Application test analysis example 1:
The test of nitrite nitrogen quick detection kit precision, concrete operations are:
The nitrite nitrogen quick detection kit for preparing is carried out repeatability, middle precision, reappearance test respectively to test sample.Replica test: in specialized range, the solution (spectrophotometric determination) of 8 different nitrite nitrogen contents of design, each concentration do 3 parallel, 3 parallel all surveys 3 times of each concentration are measured the result with gained and are estimated; Middle precision test: in this laboratory, prepare the solution of above-mentioned 8 variable concentrations with distinct device by 3 different analysts, each concentration do 3 parallel, 3 parallel all surveys 3 times of each concentration are measured the result with gained and are estimated; And the mensuration result who obtains with resulting mensuration result and replica test estimates; Reappearance test: in 2 different testing laboratories, the solution of above-mentioned 8 variable concentrations is measured by different analysts.Replica test, the test of middle precision, reappearance are tested resultant test findings and evaluated, and test findings is consistent, shows that nitrite nitrogen quick detection kit precision provided by the present invention has reached requirement fully.
Application test analysis example 2:
The test of nitrite nitrogen quick detection kit storage life:
Test of long duration: three batches of kits that prepare are put in 4 ℃, keep in Dark Place, to measuring with a collection of censorship water sample, the result shows that nitrite nitrogen content practical measurement value conforms to the spectrophotometric determination value during respectively at 0,3,6,9,12 month.
Above-mentioned detailed description of this aquaculture water nitrite nitrogen quick detection kit and detection method thereof being carried out with reference to embodiment; Be illustrative rather than determinate; Can enumerate out several embodiment according to institute's limited range; Therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (2)
1. aquaculture water nitrite nitrogen quick detection kit is characterized in that being provided with in this kit:
10ml tool plug cuvette: 1;
Colorimetric card: 1;
Suction pipe: 1;
20ml drop bottle: 2;
Reagent A: 20ml;
Reagent B:20ml;
The preparation 500ml of reagent A: measure 50ml phosphoric acid in the 200ml beaker, phosphoric acid concentration is 15mol/L=1.70g/mL, adds deionized water 200ml it is mixed; Take by weighing 20g 4-aminobenzene sulfonamide and put into said beaker, make its dissolving after, be transferred in the 500ml volumetric flask, be settled to 500ml with deionized water, packing, the 20ml/ bottle keeps in Dark Place;
The preparation 500ml of reagent B: take by weighing 1-naphthyl ethylenediamine dihydrochloride 1g and be dissolved in the 300ml deionized water, it is transferred in the 500ml volumetric flask is settled to 500ml with deionized water, packing, the 20ml/ bottle keeps in Dark Place.
2. the detection method of aquaculture water nitrite nitrogen quick detection kit according to claim 1 is characterized in that operation steps is following:
(1) washes cuvette 2～3 times with water to be checked;
(2) water sampling 5ml; Add 5 reagent A, add 5 reagent B again and shake up;
(3) build cuvette and also shake up gently, placed 1～3 minute; Cuvette is affixed on the blank space of colorimetric card, with the colorimetric card color relatively get final product the nitrite nitrogen content mg/L of the water sample of surveying.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103070112A (en) * | 2013-01-08 | 2013-05-01 | 汕头大学 | Manufacturing method of scallop color fan for measuring total carotenoid content in adductor muscles of scallops and application of scallop color fan |
| CN107064122A (en) * | 2016-12-31 | 2017-08-18 | 郑州贯奥仪器仪表有限公司 | Colorimetric reagent box and its application for detecting nitrate nitrogen in water body |
| CN107132223A (en) * | 2016-02-29 | 2017-09-05 | 福建大北农水产科技有限公司 | A kind of cultivation water nitrite quick detection kit |
| CN108426844A (en) * | 2018-03-20 | 2018-08-21 | 智锐达仪器科技南通有限公司 | A kind of quick detection reagent of nitrite in food, kit and corresponding detection method |
| CN108717043A (en) * | 2018-05-29 | 2018-10-30 | 安徽省环境监测中心站 | A kind of nitrite detection plate |
| CN109211889A (en) * | 2018-09-21 | 2019-01-15 | 深圳市长隆科技有限公司 | Fast check reagent box of nitrite nitrogen and preparation method thereof and the fast detecting method of nitrite nitrogen |
| CN109682808A (en) * | 2019-03-07 | 2019-04-26 | 济南大学 | A kind of improved faintly acid water body Central Asia nitrate nitrogen content measuring method |
| CN110261377A (en) * | 2019-07-24 | 2019-09-20 | 四川农业大学 | A kind of nitrite quick detection reagent and preparation method thereof |
| CN110849874A (en) * | 2019-12-05 | 2020-02-28 | 深圳市海聚源水产科技有限公司 | Ammonia nitrogen detection reagent formula suitable for high organic matter content culture water body and application |
| CN115575390A (en) * | 2022-09-30 | 2023-01-06 | 广东省环境科学研究院 | Combined reagent for monitoring water quality and use method thereof |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070112A (en) * | 2013-01-08 | 2013-05-01 | 汕头大学 | Manufacturing method of scallop color fan for measuring total carotenoid content in adductor muscles of scallops and application of scallop color fan |
| CN103070112B (en) * | 2013-01-08 | 2015-01-07 | 汕头大学 | Manufacturing method of scallop color fan for measuring total carotenoid content in adductor muscles of scallops and application of scallop color fan |
| CN107132223A (en) * | 2016-02-29 | 2017-09-05 | 福建大北农水产科技有限公司 | A kind of cultivation water nitrite quick detection kit |
| CN107064122A (en) * | 2016-12-31 | 2017-08-18 | 郑州贯奥仪器仪表有限公司 | Colorimetric reagent box and its application for detecting nitrate nitrogen in water body |
| CN108426844A (en) * | 2018-03-20 | 2018-08-21 | 智锐达仪器科技南通有限公司 | A kind of quick detection reagent of nitrite in food, kit and corresponding detection method |
| CN108717043A (en) * | 2018-05-29 | 2018-10-30 | 安徽省环境监测中心站 | A kind of nitrite detection plate |
| CN109211889A (en) * | 2018-09-21 | 2019-01-15 | 深圳市长隆科技有限公司 | Fast check reagent box of nitrite nitrogen and preparation method thereof and the fast detecting method of nitrite nitrogen |
| CN109682808A (en) * | 2019-03-07 | 2019-04-26 | 济南大学 | A kind of improved faintly acid water body Central Asia nitrate nitrogen content measuring method |
| CN110261377A (en) * | 2019-07-24 | 2019-09-20 | 四川农业大学 | A kind of nitrite quick detection reagent and preparation method thereof |
| CN110849874A (en) * | 2019-12-05 | 2020-02-28 | 深圳市海聚源水产科技有限公司 | Ammonia nitrogen detection reagent formula suitable for high organic matter content culture water body and application |
| CN110849874B (en) * | 2019-12-05 | 2022-10-04 | 深圳市海聚源水产科技有限公司 | Ammonia nitrogen detection reagent formula suitable for high organic matter content culture water body and application |
| CN115575390A (en) * | 2022-09-30 | 2023-01-06 | 广东省环境科学研究院 | Combined reagent for monitoring water quality and use method thereof |
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Application publication date: 20120620 |