CN102391386A - Polysaccharide extract solution of carambola, and preparation method and application thereof - Google Patents
Polysaccharide extract solution of carambola, and preparation method and application thereof Download PDFInfo
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- CN102391386A CN102391386A CN2011103339894A CN201110333989A CN102391386A CN 102391386 A CN102391386 A CN 102391386A CN 2011103339894 A CN2011103339894 A CN 2011103339894A CN 201110333989 A CN201110333989 A CN 201110333989A CN 102391386 A CN102391386 A CN 102391386A
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Abstract
The invention relates to a polysaccharide extract solution of carambola, a preparation method and an application thereof, which relates to extraction of a natural substance. The preparation method comprises the following steps of juicing or crushing a raw material; mixing the raw material with distilled water; extracting; removing residues; adding cellulose and trypsase; removing impurities; rotating and evaporating; and adding ethanol, and centrifugating and separating supernatant to obtain the polysaccharide extract solution of the carambola. The polysaccharide extract solution of the carambola can be used as a humectant in cosmetics.
Description
Technical field
The present invention relates to a kind of extraction of crude substance, especially relate to a kind of carambola polysaccharide extraction liquid and preparation method thereof and application.
Background technology
Carambola have another name called carambola, carambola, three honest and clean, carambola, three is harvested, five ribs, Oxalidaceae belongs to tropical aiphyllium, in Guangdong, resource-savings such as Fujian, Hainan are abundant.Carambola has good pharmaceutical use, puts down in writing according to Compendium of Material Medica: multiple efficacies such as carambola has wind-heat, promotes the production of body fluid to quench thirst, relieving alcoholism, hemostasis, removing toxic substances and promoting tissue regeneration.Edible and the pharmaceutical use of carambola receives people's attention day by day.The sweet mouthfeel of carambola, succulence; Sophisticated carambola fruit is bluish yellow look or reddish yellow; Contain sugar 3.5%～10%, total acid 0.36%～1.3%, protein 0.48%～0.64%, vitamins C 13.0～18.0mg/100g, and be rich in multiple micro elements needed by human.The some of them activeconstituents can be applicable to medicine and food, and in addition, the carambola polysaccharide can be used for rising in the makeup moisture-keeping function.
Chinese patent CN102140060A discloses a kind of extraction, isolating method of carambola root benzoquinones class chemical ingredients crude extract.This method is a raw material with the dry root of Oxalidaceae plant carambola; Through ethanol or water heating and refluxing extraction; Concentrate, behind hexanaphthene, ETHYLE ACETATE, n-butanol extraction, acetic acid ethyl acetate extract is obtained benzoquinones class chemical ingredients crude extract with the method separation of opening the silicagel column column chromatography again.This invention carambola root water is carried or the acetic acid ethyl acetate extract of alcohol extract to adopt trichloromethane, hexanaphthene respectively be elutriant; Method with open silicagel column column chromatography; Make carambola root benzoquinones class chemical ingredients sharp separation; This method is easy and simple to handle, and the benzoquinones class chemical composition content that is extracted is high, and cost is low.This invention can be used for extraction, the separation of laboratory carambola root benzoquinones class chemical ingredients crude extract, also can be used for extraction, the separation of carambola root benzoquinones class chemical ingredients crude extract in big the production.
Summary of the invention
The purpose of this invention is to provide a kind of carambola polysaccharide extraction liquid and preparation method thereof and application.
The preparing method's of said carambola polysaccharide extraction liquid concrete steps are following:
Raw material is squeezed the juice or pulverized, mix with zero(ppm) water, extract the back and remove residue, add cellulase and trypsinase, remove impurity, rotary evaporation adds ethanol at last, and the spinning supernatant liquid promptly gets the carambola polysaccharide extraction liquid.
Said raw material can be that fresh carambola is squeezed the juice or dried carambola, and the mass ratio of said raw material and zero(ppm) water can be 1: (0.5～3) is preferably 1: 3; The temperature of said extraction can be 30～90 ℃; Be preferably 45～55 ℃, the time of extraction can be 2～12h, is preferably 5～7h; The best rotary evaporation of said rotary evaporation is preferably 20% to 15%～25% of original volume; Said adding alcoholic acid amount can be 2.5～3.5 times of TV behind the rotary evaporation, is preferably 3 times.
Also can adopt drying process behind the spinning supernatant liquid, dry back products therefrom is mixed with the carambola polysaccharide extraction liquid again; Said drying can adopt lyophilize or vacuum-drying, and the exsiccant time can be 1～24h, is preferably 8h.
Said carambola polysaccharide extraction liquid can be used as the wetting Agent for Printing Inks in the makeup.
The preserve moisture qualitative analysis of composition of the carambola polysaccharide extraction liquid that said process for extracting is obtained detects, and its moisture-keeping efficacy is estimated through experiment and instrument detecting.
Can confirm to contain in the carambola extracting solution polysaccharide component and content thereof through the phenol sulfuric acid process.
In addition, the carambola polysaccharide extraction liquid adopts the analysis of phenolsulfuric acid method to confirm wherein to contain polysaccharide component.The phenolsulfuric acid method is to utilize polysaccharide under the vitriolic effect, to be hydrolyzed into monose earlier, and dehydration generates the alditol verivate rapidly, generates orange-yellow compound with phenol then.Utilize the phenolsulfuric acid method to confirm to contain polysaccharide component in the carambola extracting solution through the coupling reaction of carambola polysaccharide extraction liquid.The moisture-keeping function of polysaccharide is:
1) hydroxyl in the polysaccharide molecule, carboxyl and other polar groups can form hydrogen bond and combine a large amount of moisture with water molecules, and simultaneously, the polysaccharide molecule chain also is interweaved and reticulates, and with the hydrogen bonded of water, play very strong water retention in addition.In addition, in extracellular matrix, the common outer gelatinised matrix of the born of the same parents that contain large quantity of moisture of forming of other polysaccharide fractions in polysaccharide and the skin and fibrous protein is for skin provides moisture.
2) polysaccharide has good film forming properties, can form the layer of even film at skin surface, reduces the moisture evaporation of skin surface; Make moisture permeate into stratum corneum from the substrate tissue; Induce the further aquation of stratum corneum, preserve the moisture of skin self, and accomplish the skin moisten effect.Therefore, the height water-absorbent of polysaccharide and good film-forming properties ideally combine, and can good humidity-holding effect be provided for skin.
Moisture-keeping efficacy evaluation to the makeup functional mass is the height through skin water-content behind human test's method mensuration coating wetting Agent for Printing Inks.The water-content value is high more, and the moisture-retaining capacity of expression wetting Agent for Printing Inks is strong more.The measuring method of carambola polysaccharide moisture-keeping efficacy is the German CK MPA9 of the company skin test of an employing system, uses Corneometer CM825 moisture of skin test probe to measure the water-content value.
Its effect of wetting Agent for Printing Inks is to form thin film at skin surface, moisture seal evaporated to prevent that water from dividing in skin, and the absorption that does not hinder skin to divide simultaneously for water in air, thus keep the suitable humidity of skin.Cosmetic humectant at present commonly used mainly extracts the plant that skin corium and the marine alga of soft connective tissue from animal, animal etc. is rich in polyose, and the contrast as carambola polysaccharide extraction liquid humidity-holding effect of wetting Agent for Printing Inks that mucinase, collagen protein, Collagen Hydrolysate, Seaweed Extract etc. use always is adopted in this experiment.
Description of drawings
Fig. 1 is the humidity-holding effect of different mass concentration carambola polysaccharide.In Fig. 1, X-coordinate is time/h, and ordinate zou is a water-content; ■ is 2% carambola polysaccharide; ● be 4% carambola polysaccharide; ▲ be 6% carambola polysaccharide;
is 8% carambola polysaccharide;
is 10% carambola polysaccharide,
be blank.
Fig. 2 is the humidity-holding effect contrast of different wetting Agent for Printing Inkss.In Fig. 2, X-coordinate is time/h, and ordinate zou is a water-content; ■ is 4% carambola polysaccharide; ● be 0.4% mucinase; ▲ be 4% Seaweed Extract;
is 4% collagen protein;
is 4% elastin hydrolysis,
be blank.
Fig. 3 is the influence of solid-liquid ratio to carambola polysaccharide yield.In Fig. 3, X-coordinate is a solid-liquid ratio, and ordinate zou is polysaccharide yield/%; Condition is: time 6h, 50 ℃ of temperature.
Fig. 4 is the influence of extraction time to carambola polysaccharide yield.In Fig. 4, X-coordinate is time/h, and ordinate zou is polysaccharide yield/%; Condition is: 50 ℃ of temperature, expect that night was than 1.0: 3.0.
Fig. 5 is the influence of extraction temperature to carambola polysaccharide yield.In Fig. 5, X-coordinate be temperature/℃, ordinate zou is polysaccharide yield/%; Condition is: time 6h, expect that night was than 1.0: 3.0.
Embodiment
The resulting carambola polysaccharide extraction liquid of the present invention is that fresh carambola is squeezed the juice or dried, and investigates solid-liquid ratio, extraction temperature and the extraction time single-factor influence to carambola polysaccharide yield.Wherein solid-liquid ratio investigated 1: 0.5,1: 1,1: 1.5,1: 2,1: 3, temperature has been investigated 30 ℃, 50 ℃, 70 ℃, 90 ℃, the extraction time has been investigated 2h, 4h, 6h, 8h, 10h, 12h.The extraction conditions of wherein optimizing is solid-liquid ratio 1: 3,50 ℃ of temperature, extraction time 6h.Provide several embodiment at this according to the method steps in the foregoing invention content, the parameter among the embodiment must guarantee that the initial and end of each parameter area and some intermediate values all must have embodiment.
The measuring method of carambola polysaccharide extraction liquid performance of keeping humidity is the moisture-retaining capacity through human body method test mass concentration 2%, 4%, 6%, 8%, 10% carambola polysaccharide extraction liquid; And compare with the moisture-retaining capacity of 0.4% mucinase, 4% Seaweed Extract, 4% collagen protein, 4% elastin hydrolysis respectively, thereby the moisture-keeping functions of carambola polysaccharide extraction liquid is estimated.The carambola polysaccharide extraction liquid that present method adopts is in extracting experiment, obtain formulated through cryodesiccated carambola polysaccharide sample.Experiment condition is that the temperature and humidity of test period requirement environment is constant, and temperature is 22 ± 2 ℃, and relative humidity is 40%～60%.Adopt the German CK MPA9 of company skin test system, use Corneometer CM825 moisture of skin test probe that the experimenter is tested.
1, MMV (moisture measurement value) moisture content measurement value method
Adopt the hydration of Corneometer CM825 moisture of skin test probe test skin.Its action principle is because stratum corneum contains a large amount of ionogen, exists the ionogen in the water to have electric conductivity, and this phenomenon also is created in skin surface; After test probe and skin exposure; The variation of specific conductivity reflect skin hydration status (Du Xiaohao, Xu Wei, Du Xuejie. Moisture Efficiency of Skin Care Product evaluation [J]. detergents and cosmetic; 2000,30 (3): 47-52).
The carambola polysaccharide concentration to the testing method of humidity-holding effect influence is: select 10 of healthy no dermopathic trial volunteers.1, confirm 6 test zones of left and right sides forearm, region area is 2cm * 2cm, and wherein a zone is measured its hydration rate as blank contrast areas with the CM825 probe.2, mass concentration is that 2%, 4%, 6%, 8%, 10% carambola polysaccharide extraction liquid is coated on other five zones respectively as specimen.3, every separated 30min tests, and tests the end to 3h, and 5 points of every domain test are averaged.The skin that obtains coating quality concentration respectively and be 2%, 4%, 6%, 8%, 10% carambola polysaccharide extraction liquid is in 0.5h, 1h, 1.5h, 2h, 2.5h, 3h water cut.During the treating water content data 10 bit test person gained results are averaged to confirm final humidity-holding effect.Can know that by the result humidity-holding effect of carambola polysaccharide extraction liquid strengthens along with the increase of mass concentration.Mass concentration is that 10% carambola polysaccharide extraction liquid humidity-holding effect is best and humidity-holding effect stable, and is over time very little.Concrete test result is as shown in Figure 1.
Contrast experiment's testing method of carambola polysaccharide extraction liquid and other wetting Agent for Printing Inks humidity-holding effects is: select 10 of healthy no dermopathic trial volunteers.1, confirm 6 test zones of left and right sides forearm, region area is 2cm * 2cm, and wherein a zone is measured its hydration rate as blank contrast areas with the CM825 probe.2, mass concentration is that 4% carambola polysaccharide extraction liquid and 0.4% mucinase, 4% Seaweed Extract, 4% collagen protein, 4% Collagen Hydrolysate are coated on other five zones respectively as specimen.3, every separated 30min tests, and tests the end to 3h, and 5 points of every domain test are averaged.Obtain coating quality concentration respectively and be the water cut of skin when 0.5h, 1h, 1.5h, 2h, 2.5h, 3h of 4% carambola polysaccharide extraction liquid and 0.4% mucinase, 4% Seaweed Extract, 4% collagen protein, 4% Collagen Hydrolysate.During the treating water content data 10 bit test person gained results are averaged to confirm final humidity-holding effect.Can be known that by the result humidity-holding effect of 4% carambola polysaccharide extraction liquid and 4% elastin hydrolysis is best, its humidity-holding effect is superior to 0.4% mucinase, 4% Seaweed Extract, 4% collagen protein.Concrete test result is as shown in Figure 2.
Therefore the carambola polysaccharide extraction liquid is a kind of very effective wetting Agent for Printing Inks, can be used as a kind of novel wetting Agent for Printing Inks and is applied in the makeup.
Fig. 3 provides the influence of solid-liquid ratio to carambola polysaccharide yield.Fig. 4 provides the influence of extraction time to carambola polysaccharide yield.Fig. 5 provides the influence of extraction temperature to carambola polysaccharide yield.
Claims (10)
1. the preparation method of carambola polysaccharide extraction liquid is characterized in that concrete steps are following:
Raw material is squeezed the juice or pulverized, mix with zero(ppm) water, extract the back and remove residue, add cellulase and trypsinase, remove impurity, rotary evaporation adds ethanol at last, and the spinning supernatant liquid promptly gets the carambola polysaccharide extraction liquid.
2. the preparation method of carambola polysaccharide extraction liquid as claimed in claim 1 is characterized in that said raw material is that fresh carambola is squeezed the juice or dried carambola.
3. the preparation method of carambola polysaccharide extraction liquid as claimed in claim 1, the mass ratio that it is characterized in that said raw material and zero(ppm) water is 1: (0.5～3).
4. the preparation method of carambola polysaccharide extraction liquid as claimed in claim 3, the mass ratio that it is characterized in that said raw material and zero(ppm) water is for being 1: 3.
5. the preparation method of carambola polysaccharide extraction liquid as claimed in claim 1, the temperature that it is characterized in that said extraction is 30～90 ℃, the time of extraction is 2～12h.
6. the preparation method of carambola polysaccharide extraction liquid as claimed in claim 5, the temperature that it is characterized in that said extraction is 45～55 ℃, the time of extraction is 5～7h.
7. the preparation method of carambola polysaccharide extraction liquid as claimed in claim 1 is characterized in that said rotary evaporation is 15%～25% of rotary evaporation a to original volume, is preferably 20%.
8. the preparation method of carambola polysaccharide extraction liquid as claimed in claim 1 is characterized in that said adding alcoholic acid amount is 2.5～3.5 times of TV behind the rotary evaporation, is preferably 3 times.
9. like the prepared carambola polysaccharide extraction liquid of one of said preparation method in the claim 1～8.
Like the prepared carambola polysaccharide extraction liquid of one of said preparation method in the claim 1～8 as the wetting Agent for Printing Inks in the makeup.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015041514A1 (en) * | 2013-09-19 | 2015-03-26 | Forest Research Institute Malaysia | Averrhoa carambola extract and a method for producing the extract |
| CN107157892A (en) * | 2017-06-22 | 2017-09-15 | 西林县大银子铁皮石斛种销农民专业合作社 | It is a kind of to be used to repair dendrobium candidum clay mask of acne print and preparation method thereof |
| CN113501887B (en) * | 2021-04-02 | 2022-06-10 | 广西中医药大学 | Active polysaccharide of inducted grass and preparation method thereof |
| CN118001199A (en) * | 2024-02-02 | 2024-05-10 | 中科润航健康科技有限公司 | Anti-oxidation and skin tightening essence containing tri-factor tripeptide and preparation method thereof |
-
2011
- 2011-10-27 CN CN2011103339894A patent/CN102391386A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 朱银铃等: "杨桃多糖的提取研究", 《食品工业科技》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015041514A1 (en) * | 2013-09-19 | 2015-03-26 | Forest Research Institute Malaysia | Averrhoa carambola extract and a method for producing the extract |
| CN107157892A (en) * | 2017-06-22 | 2017-09-15 | 西林县大银子铁皮石斛种销农民专业合作社 | It is a kind of to be used to repair dendrobium candidum clay mask of acne print and preparation method thereof |
| CN113501887B (en) * | 2021-04-02 | 2022-06-10 | 广西中医药大学 | Active polysaccharide of inducted grass and preparation method thereof |
| CN118001199A (en) * | 2024-02-02 | 2024-05-10 | 中科润航健康科技有限公司 | Anti-oxidation and skin tightening essence containing tri-factor tripeptide and preparation method thereof |
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Application publication date: 20120328 |