CN102334602A - Preparation method of bioactive composite high-efficiency feed nutrient solution used for livestock and poultry - Google Patents
Preparation method of bioactive composite high-efficiency feed nutrient solution used for livestock and poultry Download PDFInfo
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- CN102334602A CN102334602A CN2011101785674A CN201110178567A CN102334602A CN 102334602 A CN102334602 A CN 102334602A CN 2011101785674 A CN2011101785674 A CN 2011101785674A CN 201110178567 A CN201110178567 A CN 201110178567A CN 102334602 A CN102334602 A CN 102334602A
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Abstract
The invention discloses a preparation method of bioactive composite high-efficiency feed nutrient solution used for livestock and poultry. The method comprises the following steps of: inoculating bacillus coprophilus, streptococcus thermophilus, saccharomyces cerevisiae and hansenulaanomala into culture mediums to obtain four seed solutions respectively; filling 0.6-1.5 percent by weight of each of four seed solutions into an active nutrient solution which is prepared from 10-18 percent by weight of cane molasses, 0.5-1.8 percent by weight of milt cream, 10-20 percent by weight of starch and pure water through fermentation; and the feed nutrient solution is obtained after fermentation. Through the feed nutrient solution, the problems of high probability of moistening and oxidation, low probability of absorption in bodies of livestock and poultry, low material utilization rate and the like existing in the use of a solid additive are improved; a huge number of organic compounds can be decomposed; antibiotic and ablastin are produced; a large amount of amino acid, protein, vitamins, various bioactive enzymes and the like is excreted; and the feed nutrient solution has the functions of adjusting and improving functions of organs in the bodies of the livestock and poultry, increasing content of nutritive materials of the livestock and poultry, improving immunity of organisms and promoting growth and development.
Description
Technical field
The present invention relates to a kind of preparation method of livestock and poultry cultivation feed addictive, especially the biologically active composite highly effective feed nutrition liquid and preparation method thereof of livestock and poultry use.
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Background technology
Current; The feed addictive that is used for livestock and poultry cultivation is commonly auxotype and non-nutritive additive; Such as amino acid, vitamin, mineral element, antibiotic, antibiotic, hormone, enzyme preparation, anthelmintic drug, phagostimulant etc. almost is pulvis entirely, and the oxidation of pulvis moisture-sensitive is difficult in the livestock and poultry body absorbing; Because mechanical mixture is irregular, bioavailability is low.In the prior art; Being called " biology feed additive and preparation method thereof ", application number like name is 00133695.9 application for a patent for invention document; Disclose by screening and carried lactobacillus viridescens, the pure bacterium of lactobacillus coprophilus, saccharomycete, bacillus subtilis, heap fermentation, oven dry and reach the biology feed additive of bacterial classification quantity 8-10 hundred million/gram through an enlarged culture of culture medium, secondary enlarged culture, with rice bran, wheat bran absorption bacterium liquid; Can improve the function of livestock and poultry animal enteron aisle digestive system; Make flora equilibrium in the enteron aisle, reduce the antibiotics use amount, improve the quality of animal products; Improve absorption and the conversion ratio of livestock and poultry, save feed, reduce feed cost etc. feed; But this kind feed addictive, in adjustment and improve livestock and poultry intracorporeal organ function, increase the raising poultry nutritive content of material, improve immunity of organism, aspect such as promote to grow, the part that still has some deficits needs to improve energetically, could satisfy social requirement to animal products.
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Summary of the invention
The present invention is directed to the above-mentioned deficiency that prior art livestock and poultry cultivation feed addictive exists, the biologically active composite highly effective feed nutrition liquid and preparation method thereof that provides a kind of livestock and poultry that can adjust and improve livestock and poultry intracorporeal organ function, increase raising poultry nutritive content of material to use.
Technical scheme of the present invention: the biologically active composite highly effective feed nutrition liquid and preparation method thereof that livestock and poultry are used is characterized in that:
One, the mother's kind with lactobacillus coprophilus and streptococcus thermophilus is inoculated in activation medium respectively:
(1) activation medium of lactobacillus coprophilus:
Peptone (Proteose peptone) 5-15g,
Beef extracts thing (Beet extract) 5-15g,
Yeast extract (Yeast extract) 3-8g,
Glucose (Dextrose) 15-32g,
Sodium acetate (Sodium acetacte) 3-8g,
Manganese sulfate (Manganese sulfate) 0.01-0.1g,
Potassium phosphate (Dipotassium phosphate) 1-5g,
Distilled water (Distilled water) 50-200ml,
After the above-mentioned material stirring and dissolving, adjust pH value to 6.0-6.5, under 110-130 ℃ of temperature, sterilize a period of time; Then, culture medium is cooled to 60 ℃ or following, adds carbohydrate through membrane filtration, sterilization; Make in the culture medium carbohydrate ultimate density be 1.2% or below; Divide in the spiral test tube of the bacteria reducing of packing into, a period of time in that culture process below 40 ℃ requires, be the lactobacillus coprophilus seed liquor.Described yeast extract is the deposit behind the saccharomycete seed liquid sterilizing, drying.
(2) activation medium of Streptomyces thermophilus:
Peptone 5-15g,
Beef extracts thing 0.5-2g
Sodium chloride 3-8g,
Bromocresol purple 0.01-0.025g,
Distilled water 50-200ml,
After the above-mentioned material stirring and dissolving, adjustment pH value to 6.0-6.5 is sterilized a period of time under 110-130 ℃ of temperature; Treat that culture medium is cooled to 60 ℃ or following; Add carbohydrate through membrane filtration, sterilization, make in the culture medium concentration of saccharide be 1.2% or below, be sub-packed in the test tube of sterilizing; A period of time in that culture process below 40 ℃ requires, be thermophilic newborn bar chain coccus seed liquor.
Two, preparation saccharomyces cerevisiae, unusual Hansenula yeast culture medium:
Potato 200-500g,
Glucose 6-15g,
Agar 6-15g,
The root powder 12-30g that faints,
Yeast extract 3-8g,
Hops 12-30g,
Distilled water 600-2000ml;
After above-mentioned material is ready for,, adds distilled water and boil 10min-20min the peeling potatoes stripping and slicing; Use filtered through gauze; Adding distil water then adds glucose, ball fat, chrysanthemum root powder, yeast extract, hops by aforementioned dosage again, and heating is dissolved, pH value is adjusted to 6.5-7.0; Under 110-130 ℃ of temperature, sterilize a period of time after the packing, promptly obtain culture medium; Described yeast extract is the deposit behind the saccharomycete seed liquid sterilizing, drying.
Saccharomyces cerevisiae and unusual Hansenula yeast are inoculated in respectively in this culture medium, obtain S. cervisiae seed liquor and unusual Hansenula yeast bacterium seed liquor.
Three, make active nutrient liquid: its component and percentage by weight are:
Cane molasses 10-18%, milt cream 0.5-1.8%, starch 10-20%, remainder is a pure water, the cane molasses that takes by weighing in proportion, milt cream, starch is placed in the fermentation tank of sterilization treatment; Inject pure water, boiling to 100 then ℃ is when being cooled to 32 ℃-35 ℃ again; With aforementioned lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae; Each puts into unusual Hansenula yeast bacterium seed liquor with 0.6-1.5% part by weight, and a period of time that fermentation tank requires with anaerobism ferment mode culture process, the adjustment pH value remains on 5-5.5; Promptly accomplish fermentation, obtain the high-efficiency total nutrient nonreactive feed nutrition liquid that livestock and poultry of the present invention are used.
Further characteristic is: lactobacillus coprophilus, and streptococcus thermophilus, S. cervisiae, unusual Hansenula yeast bacterium seed liquor addition respectively is 1%.
The biologically active composite highly effective feed nutrition liquid preparation method that livestock and poultry of the present invention are used with respect to prior art, has following characteristics:
1, feed nutrition liquid of the present invention be feed addictive novel form, new varieties; Improve and problem such as be difficult for absorbing, the thing utilization rate is low in moisture-sensitive oxidation that solid additive exists in using, the livestock and poultry body, it can the huge organic double compound of split amount, produces antibiotic and ablastins; Secrete a large amount of amino acid, protein, vitamin, various bioactive enzymes etc.; Have adjustment and improve livestock and poultry intracorporeal organ function, increase the raising poultry nutritive content of material, improve immunity of organism; The function that promotion is grown, thus satisfy the new demand of society to animal products.
2, preparation method of the present invention is simple, and material therefor is also obtained easily, can reduce the feed consumption, reduce feeding cost.
The specific embodiment
The composition and the preparation method thereof of the high-efficiency total nutrient nonreactive feed nutrition liquid that livestock and poultry of the present invention are used are following:
1. the mother's kind with lactobacillus coprophilus (Lactobacillus coprophilus) and streptococcus thermophilus (Streptococcus thermophilus) is inoculated in activation medium respectively:
(1) lactobacillus coprophilus activation medium:
Peptone (Proteose peptone) 5-15g,
Beef extracts thing (Beet extract) 5-15g,
Yeast extract (Yeast extract) 3-8g,
Glucose (Dextrose) 15-32g
Sodium acetate (Sodium acetacte) 3-8g,
Manganese sulfate (Manganese sulfate) 0.01-0.1g,
Potassium phosphate (Dipotassium phosphate) 1-5g,
Distilled water (Distilled water) 50-200ml,
After the above-mentioned material stirring and dissolving, adjust pH value to 6.0-6.5 (hydrochloric acid commonly used or NaOH adjustment pH value), under 110-130 ℃ of temperature, sterilize a period of time, as 10-20 minutes; Then, culture medium is cooled to 60 ℃ or following, adds the filter membrane filtration through tiny aperture, the carbohydrate of sterilization; The aperture of the filter membrane that the present invention chooses is 0.22 μ m or littler, make in the culture medium carbohydrate ultimate density be 1.2% or below, divide in the spiral test tube of the bacteria reducing of packing into; Test tube like 5ml; In a period of time that culture process below 40 ℃ requires,, be the lactobacillus coprophilus seed liquor like 37 ℃ of cultivations 72 hours.Described yeast extract is the deposit behind the saccharomycete seed liquid sterilizing, drying.It is beef that described beef extracts thing, or the material behind the beef drying and dewatering.
(2) Streptomyces thermophilus (Streptococcus thermophilus) activation medium:
Peptone (Proteose peptone) 5-15g,
Beef extracts thing (Beet extract) 0.5-2g
Sodium chloride (Sodium chloride) 3-8g,
Bromocresol purple (Bromcresol purple) 0.01-0.025g,
Distilled water (Distilled water) 50-200ml,
After the above-mentioned material stirring and dissolving, adjustment pH value to 6.0-6.5 (hydrochloric acid commonly used or NaOH adjustment pH value) is sterilized a period of time under 110-130 ℃ of temperature, as 10-20 minutes, as, sterilized 15 minutes for 121 ℃; Treat that culture medium is cooled to 60 ℃ or following, add the filter membrane filtration through tiny aperture, the carbohydrate of sterilization, the aperture of the filter membrane that the present invention chooses is 0.22 μ m or littler; Make in the culture medium concentration of saccharide be 1.2% or below; Be sub-packed in the test tube of sterilizing, like the test tube of 4ml, in a period of time that culture process below 40 ℃ requires; Like 37 ℃ of cultivations 48 hours, be thermophilic newborn bar chain coccus seed liquor.
2. saccharomyces cerevisiae (Saccharomyces cereviside), unusual Hansenula yeast (Hansenula arabitolgenes) culture medium:
Potato 200-500g,
Glucose 6-15g,
Agar 6-15g,
The root powder 12-30g that faints,
Yeast extract 3-8g,
Hops 12-30g,
Distilled water 600-2000ml;
After above-mentioned material is ready for,, adds distilled water and boil 10min-20min, use filtered through gauze the peeling potatoes stripping and slicing; Adding distil water can be added to 1000ml again, then adds glucose by aforementioned dosage (in proportion); Ball fat, faint root powder, yeast extract, hops, heating is dissolved, pH value is adjusted to 6.5-7.0 (hydrochloric acid commonly used or NaOH adjustment pH values); Under 110-130 ℃ of temperature, sterilize a period of time after the packing,, promptly obtain culture medium like sterilization 30min;
Saccharomyces cerevisiae and unusual Hansenula yeast are inoculated in respectively in this culture medium, obtain S. cervisiae seed liquor and unusual Hansenula yeast bacterium seed liquor.
3. first with the lactobacillus coprophilus of processing, streptococcus thermophilus, S. cervisiae, unusual Hansenula yeast bacterium seed liquor is subsequent use.
4. making active nutrient liquid: its component and percentage by weight are:
Cane molasses 10-18%, milt cream 0.5-1.8%, starch 10-20%, remainder is a pure water, and the cane molasses that takes by weighing in proportion, milt cream, starch are placed in the sterilization treatment fermentation tank; Inject pure water, boiling to 100 then ℃ is when being cooled to 32 ℃-35 ℃ (fermentation tank thermostatic control) again; With aforementioned lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae; Each puts into (ratio that accounts for the active nutrient liquid gross weight) with 0.6-1.5% part by weight unusual Hansenula yeast bacterium seed liquor; The a period of time that in desinfection chamber, requires with anaerobism ferment mode culture process in the fermentation tank, as about 14 days, the adjustment pH value remains on 5-5.5; Promptly accomplish fermentation, obtain the high-efficiency total nutrient nonreactive feed nutrition liquid that livestock and poultry of the present invention are used.
Lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae, unusual Hansenula yeast bacterium seed liquor addition, every kind of the best is 1%.
The embodiment of concrete utilization is following:
Aspect animal and fowl fodder, each ton feed adds 1kg-2kg nutrient solution of the present invention, with this nutrient solution dilution 15-25 doubly, then dilution is evenly sneaked into feeding in the feed before using.
Aspect drinking-water, each ton drinking water uses 0.5kg-1kg nutrient solution of the present invention (in 24 hours, using up).Waste when avoiding livestock and fowl drinking water can be made a water collector down in water fountain, collects and spills drinking-water, is used for the sprinkling of bedding and padding at ordinary times and preserves moisture.
The main effect of nutrient solution of the present invention, livestock and poultry physique is improved in (1), promotes immunologic function.This nutrition fluid power activates the activity of various enzymes in the livestock and poultry body, can effectively reduce disease and take place, and improves immunity, reaches the effect of disease-resistant, diseases prevention and minimizing morbidity.(2) improve food conversion ratio, accelerate growth of animals or poultry, reduce cost.This nutrient solution biologic ferment can be strengthened intestines peristalsis and secretion, decomposes larger molecular organics matter in the feed, promotes feed digestion, and produces multiple digestive ferment and make feed nutrient more be prone to be absorbed.Probio is decomposed self bacterium protein, for livestock and poultry provide more nutrient, promotes effectively to grow and improve production performance, increases daily gain and price of deed rate, shortens the feed cycle.(3) prevent diarrhoea, increase appetite.Effectively improve microbial environment in the animal and bird intestines, the system of helping digest impels food fully to digest and absorbs, and then reduces the generation of diarrhoea and disease of digestive tract, especially yellow, the dysentery characterized by white mucous stool of piglet.(4) improve livestock and poultry meat matter, reduce feedstuff-meat ratio.Because of a large amount of beneficial microbe activations are arranged in the livestock and poultry body, can impel meat to improve, feedstuff-meat ratio decline 10%-20% becomes the nuisanceless meat of standard.(5) breeding potential obviously improves.Preceding 20 days these nutrient solutions that begin to feed (containing feed, drinking-water) of live pig mating sow fertility rate can improve about 30%-40%.
Nutrient solution of the present invention is used for livestock and poultry cultivation; Can the huge organic double compound of split amount; Produce antibiotic and ablastins, secrete a large amount of amino acid, protein, vitamin, various bioactive enzymes etc., have the function of adjustment and raising livestock and poultry intracorporeal organ, enhance immunity.
Claims (2)
1. the biologically active composite highly effective feed nutrition liquid and preparation method thereof that uses of livestock and poultry is characterized in that:
One, the mother's kind with lactobacillus coprophilus and streptococcus thermophilus is inoculated in activation medium respectively:
(1) activation medium of lactobacillus coprophilus:
Peptone (Proteose peptone) 5-15g,
Beef extracts thing (Beet extract) 5-15g,
Yeast extract (Yeast extract) 3-8g,
Glucose (Dextrose) 15-32g,
Sodium acetate (Sodium acetacte) 3-8g,
Manganese sulfate (Manganese sulfate) 0.01-0.1g,
Potassium phosphate (Dipotassium phosphate) 1-5g,
Distilled water (Distilled water) 50-200ml,
After the above-mentioned material stirring and dissolving, adjust pH value to 6.0-6.5, under 110-130 ℃ of temperature, sterilize a period of time; Then, culture medium is cooled to 60 ℃ or following, adds carbohydrate through membrane filtration, sterilization; Make in the culture medium carbohydrate ultimate density be 1.2% or below; Divide in the spiral test tube of the bacteria reducing of packing into, a period of time in that culture process below 40 ℃ requires, be the lactobacillus coprophilus seed liquor;
(2) activation medium of Streptomyces thermophilus:
Peptone 5-15g,
Beef extracts thing 0.5-2g
Sodium chloride 3-8g,
Bromocresol purple 0.01-0.025g,
Distilled water 50-200ml,
After the above-mentioned material stirring and dissolving, adjustment pH value to 6.0-6.5 is sterilized a period of time under 110-130 ℃ of temperature; Treat that culture medium is cooled to 60 ℃ or following; Add carbohydrate through membrane filtration, sterilization, make in the culture medium concentration of saccharide be 1.2% or below, be sub-packed in the test tube of sterilizing; A period of time in that culture process below 40 ℃ requires, be thermophilic newborn bar chain coccus seed liquor;
Two, preparation saccharomyces cerevisiae, unusual Hansenula yeast culture medium:
Potato 200-500g,
Glucose 6-15g,
Agar 6-15g,
The root powder 12-30g that faints,
Yeast extract 3-8g,
Hops 12-30g,
Distilled water 600-2000ml;
After above-mentioned material is ready for,, adds distilled water and boil 10min-20min the peeling potatoes stripping and slicing; Use filtered through gauze; Adding distil water then adds glucose, ball fat, faint root powder, yeast extract, hops by aforementioned dosage again, and heating is dissolved, pH value is adjusted to 6.5-7.0; Under 110-130 ℃ of temperature, sterilize a period of time after the packing, promptly obtain culture medium;
Saccharomyces cerevisiae and unusual Hansenula yeast are inoculated in respectively in this culture medium, obtain S. cervisiae seed liquor and unusual Hansenula yeast bacterium seed liquor;
Three, make active nutrient liquid: its component and percentage by weight are:
Cane molasses 10-18%, milt cream 0.5-1.8%, starch 10-20%, remainder is a pure water, the cane molasses that takes by weighing in proportion, milt cream, starch is placed in the fermentation tank of sterilization treatment; Inject pure water, boiling to 100 then ℃ is when being cooled to 32 ℃-35 ℃ again; With aforementioned lactobacillus coprophilus, streptococcus thermophilus, S. cervisiae; Each puts into unusual Hansenula yeast bacterium seed liquor with 0.6-1.5% part by weight, and a period of time that fermentation tank requires with anaerobism ferment mode culture process, the adjustment pH value remains on 5-5.5; Promptly accomplish fermentation, obtain the high-efficiency total nutrient nonreactive feed nutrition liquid that livestock and poultry of the present invention are used.
2. the biologically active composite highly effective feed nutrition liquid and preparation method thereof that uses according to the said livestock and poultry of claim 1 is characterized in that: lactobacillus coprophilus, and streptococcus thermophilus, S. cervisiae, unusual Hansenula yeast bacterium seed liquor addition is 1%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102616917A (en) * | 2012-03-13 | 2012-08-01 | 广东金海润生物科技有限公司 | Amino acid nutrient solution and application thereof in aquaculture |
| CN103113142A (en) * | 2013-01-30 | 2013-05-22 | 重庆富博生物技术有限公司 | Deodorized fermentation liquor of excrements of livestocks and preparation method thereof |
| CN105595010A (en) * | 2015-09-01 | 2016-05-25 | 嘉兴佳康丽生物科技有限公司 | Water quality balance ferment for aquaculture and organic sewage treatment and preparation method thereof |
| CN105861615A (en) * | 2016-04-22 | 2016-08-17 | 闫大凤 | Production method of amino acid bioactive peptide for aquatic products |
| CN108040888A (en) * | 2017-12-11 | 2018-05-18 | 嘉兴佳康丽生物科技有限公司 | A kind of livestock and poultry cultivation active compound probiotic nutrition ferment and preparation method |
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| CN1800357A (en) * | 2005-12-12 | 2006-07-12 | 青岛地恩地生物科技有限公司 | Composite bacterium agent for organic material fermentation |
| CN101341927A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive liquid agent for pig and preparation technique |
| CN101731460A (en) * | 2008-11-05 | 2010-06-16 | 大连春天生物技术有限公司 | Edible mushroom, spent mushroom substrate and probiotics-containing nutrient solution for livestock and poultry and preparation method thereof |
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| CN1296765A (en) * | 2000-12-04 | 2001-05-30 | 鞍山天胜旺科技开发有限公司 | Biological feed additive and its preparing process |
| CN1800357A (en) * | 2005-12-12 | 2006-07-12 | 青岛地恩地生物科技有限公司 | Composite bacterium agent for organic material fermentation |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102616917A (en) * | 2012-03-13 | 2012-08-01 | 广东金海润生物科技有限公司 | Amino acid nutrient solution and application thereof in aquaculture |
| CN103113142A (en) * | 2013-01-30 | 2013-05-22 | 重庆富博生物技术有限公司 | Deodorized fermentation liquor of excrements of livestocks and preparation method thereof |
| CN105595010A (en) * | 2015-09-01 | 2016-05-25 | 嘉兴佳康丽生物科技有限公司 | Water quality balance ferment for aquaculture and organic sewage treatment and preparation method thereof |
| CN105861615A (en) * | 2016-04-22 | 2016-08-17 | 闫大凤 | Production method of amino acid bioactive peptide for aquatic products |
| CN108040888A (en) * | 2017-12-11 | 2018-05-18 | 嘉兴佳康丽生物科技有限公司 | A kind of livestock and poultry cultivation active compound probiotic nutrition ferment and preparation method |
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| CN102334602B (en) | 2013-04-17 |
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