CN102192932B - Normal pulse stripping method for detecting content of vitamin in blood samples - Google Patents
Normal pulse stripping method for detecting content of vitamin in blood samples Download PDFInfo
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- CN102192932B CN102192932B CN 201110070651 CN201110070651A CN102192932B CN 102192932 B CN102192932 B CN 102192932B CN 201110070651 CN201110070651 CN 201110070651 CN 201110070651 A CN201110070651 A CN 201110070651A CN 102192932 B CN102192932 B CN 102192932B
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- vitamin
- content
- blood samples
- blood sample
- sample
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- 229940088594 vitamin Drugs 0.000 title claims abstract description 60
- 229930003231 vitamin Natural products 0.000 title claims abstract description 60
- 235000013343 vitamin Nutrition 0.000 title claims abstract description 60
- 239000011782 vitamin Substances 0.000 title claims abstract description 60
- 150000003722 vitamin derivatives Chemical class 0.000 title claims abstract description 59
- 239000008280 blood Substances 0.000 title claims abstract description 57
- 210000004369 blood Anatomy 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title abstract description 13
- 239000000523 sample Substances 0.000 claims abstract description 91
- 238000006479 redox reaction Methods 0.000 claims abstract description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 32
- 238000004070 electrodeposition Methods 0.000 claims description 26
- 238000002386 leaching Methods 0.000 claims description 25
- 239000000203 mixture Substances 0.000 claims description 21
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 16
- 230000000284 resting effect Effects 0.000 claims description 13
- 238000012360 testing method Methods 0.000 claims description 13
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 12
- OKIZCWYLBDKLSU-UHFFFAOYSA-M N,N,N-Trimethylmethanaminium chloride Chemical compound [Cl-].C[N+](C)(C)C OKIZCWYLBDKLSU-UHFFFAOYSA-M 0.000 claims description 8
- 235000019270 ammonium chloride Nutrition 0.000 claims description 8
- 230000005477 standard model Effects 0.000 claims description 7
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 5
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 4
- HPNMFZURTQLUMO-UHFFFAOYSA-N diethylamine Chemical compound CCNCC HPNMFZURTQLUMO-UHFFFAOYSA-N 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 20
- 230000035945 sensitivity Effects 0.000 abstract description 13
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- 239000011719 vitamin A Substances 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 abstract description 3
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 37
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 36
- 239000000243 solution Substances 0.000 description 28
- 239000002253 acid Substances 0.000 description 25
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 description 19
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 16
- 239000004094 surface-active agent Substances 0.000 description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 10
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 9
- 229910017604 nitric acid Inorganic materials 0.000 description 9
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 7
- HHEFNVCDPLQQTP-UHFFFAOYSA-N ammonium perchlorate Chemical compound [NH4+].[O-]Cl(=O)(=O)=O HHEFNVCDPLQQTP-UHFFFAOYSA-N 0.000 description 7
- AXZAYXJCENRGIM-UHFFFAOYSA-J dipotassium;tetrabromoplatinum(2-) Chemical compound [K+].[K+].[Br-].[Br-].[Br-].[Br-].[Pt+2] AXZAYXJCENRGIM-UHFFFAOYSA-J 0.000 description 7
- MHCFAGZWMAWTNR-UHFFFAOYSA-M lithium perchlorate Chemical compound [Li+].[O-]Cl(=O)(=O)=O MHCFAGZWMAWTNR-UHFFFAOYSA-M 0.000 description 7
- 229910001486 lithium perchlorate Inorganic materials 0.000 description 7
- 229910001487 potassium perchlorate Inorganic materials 0.000 description 7
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- 229940095064 tartrate Drugs 0.000 description 7
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 6
- RBWNDBNSJFCLBZ-UHFFFAOYSA-N 7-methyl-5,6,7,8-tetrahydro-3h-[1]benzothiolo[2,3-d]pyrimidine-4-thione Chemical compound N1=CNC(=S)C2=C1SC1=C2CCC(C)C1 RBWNDBNSJFCLBZ-UHFFFAOYSA-N 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
- MPCRDALPQLDDFX-UHFFFAOYSA-L Magnesium perchlorate Chemical compound [Mg+2].[O-]Cl(=O)(=O)=O.[O-]Cl(=O)(=O)=O MPCRDALPQLDDFX-UHFFFAOYSA-L 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000008351 acetate buffer Substances 0.000 description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 6
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 5
- 239000001103 potassium chloride Substances 0.000 description 5
- 235000011164 potassium chloride Nutrition 0.000 description 5
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 4
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 4
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 4
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 4
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 4
- 235000014113 dietary fatty acids Nutrition 0.000 description 4
- JGUQDUKBUKFFRO-CIIODKQPSA-N dimethylglyoxime Chemical compound O/N=C(/C)\C(\C)=N\O JGUQDUKBUKFFRO-CIIODKQPSA-N 0.000 description 4
- 239000000194 fatty acid Substances 0.000 description 4
- 229930195729 fatty acid Natural products 0.000 description 4
- 150000004665 fatty acids Chemical class 0.000 description 4
- 229950008882 polysorbate Drugs 0.000 description 4
- 229920000136 polysorbate Polymers 0.000 description 4
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 4
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 4
- 229940045997 vitamin a Drugs 0.000 description 4
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 3
- ABSPRNADVQNDOU-UHFFFAOYSA-N Menaquinone 1 Natural products C1=CC=C2C(=O)C(CC=C(C)C)=C(C)C(=O)C2=C1 ABSPRNADVQNDOU-UHFFFAOYSA-N 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 235000021355 Stearic acid Nutrition 0.000 description 3
- 229930003268 Vitamin C Natural products 0.000 description 3
- 229930003427 Vitamin E Natural products 0.000 description 3
- 239000012670 alkaline solution Substances 0.000 description 3
- 239000008366 buffered solution Substances 0.000 description 3
- 230000002950 deficient Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 3
- -1 neopelex Chemical compound 0.000 description 3
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 3
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 3
- MBWXNTAXLNYFJB-NKFFZRIASA-N phylloquinone Chemical compound C1=CC=C2C(=O)C(C/C=C(C)/CCC[C@H](C)CCC[C@H](C)CCCC(C)C)=C(C)C(=O)C2=C1 MBWXNTAXLNYFJB-NKFFZRIASA-N 0.000 description 3
- 235000019175 phylloquinone Nutrition 0.000 description 3
- 239000011772 phylloquinone Substances 0.000 description 3
- 229960001898 phytomenadione Drugs 0.000 description 3
- AVTYONGGKAJVTE-OLXYHTOASA-L potassium L-tartrate Chemical compound [K+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O AVTYONGGKAJVTE-OLXYHTOASA-L 0.000 description 3
- 239000004323 potassium nitrate Substances 0.000 description 3
- 235000010333 potassium nitrate Nutrition 0.000 description 3
- 239000001472 potassium tartrate Substances 0.000 description 3
- 229940111695 potassium tartrate Drugs 0.000 description 3
- 235000011005 potassium tartrates Nutrition 0.000 description 3
- VZOPRCCTKLAGPN-ZFJVMAEJSA-L potassium;sodium;(2r,3r)-2,3-dihydroxybutanedioate;tetrahydrate Chemical compound O.O.O.O.[Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O VZOPRCCTKLAGPN-ZFJVMAEJSA-L 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 235000002639 sodium chloride Nutrition 0.000 description 3
- 229940074446 sodium potassium tartrate tetrahydrate Drugs 0.000 description 3
- 239000008117 stearic acid Substances 0.000 description 3
- 235000019154 vitamin C Nutrition 0.000 description 3
- 239000011718 vitamin C Substances 0.000 description 3
- 235000019165 vitamin E Nutrition 0.000 description 3
- 239000011709 vitamin E Substances 0.000 description 3
- 229940046009 vitamin E Drugs 0.000 description 3
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 2
- 239000005695 Ammonium acetate Substances 0.000 description 2
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
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- 229930003471 Vitamin B2 Natural products 0.000 description 2
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- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 2
- UGSCTJJUUHWKRM-UHFFFAOYSA-L [K+].N.C(=O)([O-])C(O)C(O)C(=O)[O-].[K+] Chemical compound [K+].N.C(=O)([O-])C(O)C(O)C(=O)[O-].[K+] UGSCTJJUUHWKRM-UHFFFAOYSA-L 0.000 description 2
- 235000019257 ammonium acetate Nutrition 0.000 description 2
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- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 2
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 2
- 238000005558 fluorometry Methods 0.000 description 2
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- 230000008569 process Effects 0.000 description 2
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- 230000009467 reduction Effects 0.000 description 2
- 229960002477 riboflavin Drugs 0.000 description 2
- HELHAJAZNSDZJO-OLXYHTOASA-L sodium L-tartrate Chemical compound [Na+].[Na+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O HELHAJAZNSDZJO-OLXYHTOASA-L 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
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- 229910052938 sodium sulfate Inorganic materials 0.000 description 2
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- 238000002798 spectrophotometry method Methods 0.000 description 2
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- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 235000010374 vitamin B1 Nutrition 0.000 description 2
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- 239000011716 vitamin B2 Substances 0.000 description 2
- 235000019158 vitamin B6 Nutrition 0.000 description 2
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- 235000019166 vitamin D Nutrition 0.000 description 2
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- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
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- 230000015271 coagulation Effects 0.000 description 1
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- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
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- 229910052739 hydrogen Inorganic materials 0.000 description 1
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- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical compound [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 description 1
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- 239000003960 organic solvent Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
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- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a normal pulse stripping method for detecting the content of vitamin in blood samples, belonging to the technical field of vitamin analysis and detection. The method is characterized by comprising the following steps: taking blood samples to be detected, mixing the blood samples to be detected with a vitamin sample treatment solution, and detecting current signals generated when the blood samples are subjected to oxidation reduction reaction on a sensor probe of a vitamin detector by using the normal pulse stripping method; and making a standard curve through comparing the current signal values of standard samples with different vitamin concentrations, then obtaining the content of the vitamin in the blood samples to be detected according to the current signal values generated by the samples to be detected. The method provided by the invention has the advantages of high sensitivity, good accuracy, simplicity and convenience in operation, quickness, wide application range, suitability for being used in medical and health departments so as to carry out analysis and detection on vitamins A, B1, B2, B6, B9, B12, C, D, E, K1 and K3 in blood samples, and the like; and the method can be used for rapidly detecting the content of the vitamin in the blood samples.
Description
Technical field
The invention belongs to the vitamin technical field of analysis and detection, particularly relate to a kind of conventional pulse leaching that detects the blood sample vitamin content.
Background technology
Vitamin (vitamin) be body be keep normal physiological function must be by a class micro-content organism of food intake, regulating metabolism and keeping aspect such as physiological function and bringing into play important effect.Long-term lacking or certain vitamin of excessive absorption all can cause corresponding disease.As yctalopia, scheroma and dry skin can appear in the A that is deficient in vitamin; The D that is deficient in vitamin can suffer from rickets; The B12 that is deficient in vitamin can suffer from pernicious anaemia; The vitamin E of excess intake can destroy coagulation function, increases hemorrhage possibility; Taking in the vitamin C that surpasses 1000 milligrams can influence the functions of expelling toxin of kidney.
At present, instrument and the detection method of human body vitamin content are had nothing in common with each other, and mainly contain microbial method, ultraviolet spectrophotometry, fluorometry, high performance liquid chromatography etc.Loaded down with trivial details consuming time, the more organic solvent of needs of pretreatment technology in the high performance liquid chromatography, and the serum requirement is bigger.The vitamin kind that ultraviolet spectrophotometry, fluorometry can detect is less.The instrument that is applied to the vitamin detection at present has: Korea S Younglin company has developed vitameter, utilizes the various vitamins in efficient liquid phase process detection food, the medicine; Germany visits a R-Biopharm company and utilizes fluorescence method to produce the instrument that detects vitamin.Because instruments such as high performance liquid chromatograph, fluorescence analyser are expensive, and need the technical skill personnel to operate, detection method is numerous and diverse, and detection time is longer, so be difficult for promoting.
Summary of the invention
The present invention provides a kind of conventional pulse leaching that detects the blood sample vitamin content for solving the technical matters that exists in the known technology.
The purpose of this invention is to provide the vitamin electrochemical analysis method in a kind of blood sample, have highly sensitive, characteristics such as accuracy good, easy and simple to handle, quick, applied range, be suitable for medical department and use, can carry out the conventional pulse leaching of the detection blood sample vitamin content of characteristics such as analyzing and testing to the vitamin A in the blood sample, B1, B2, B6, B9, B12, C, D, E, K1, K3.
Implementation procedure of the present invention: get a certain amount of blood sample and mix with a certain amount of homemade vitamin sample treating fluid, utilize conventional pulse leaching to make the vitamin in the blood sample at homemade sensor probe oxidation (reduction) reaction take place, produce current signal.By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the testing sample.
The conventional pulse leaching that the present invention detects the blood sample vitamin content for the technical scheme that solves the technical matters that exists in the known technology and take is:
A kind of conventional pulse leaching that detects the blood sample vitamin content, be characterized in: get blood sample to be measured and mix with vitamin sample treating fluid, the current signal that utilizes conventional pulse leaching test sample when redox reaction takes place vitamin detector sensor probe electrode, to produce; By comparing the current signal value of variable concentrations vitamin standard model, the typical curve of formulation according to the current signal value that testing sample produces, obtains the content of vitamin in the blood sample to be measured.
The present invention detects the conventional pulse leaching of blood sample vitamin content can also take following technical scheme:
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin A content, the enrichment electro-deposition current potential of detector is-200～400mV, the enrichment electrodeposition time is 10～90s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 2～15mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.The vitamin A sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.05～10mol/L; Sodium perchlorate, potassium perchlorate, lithium perchlorate, ammonia perchlorate be perchlorate most preferably.Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～5mol/L; Nitric acid, perchloric acid be strong acid most preferably.The addition of dimethyl formamide is 1～100mmol/L.
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B1 content, the enrichment electro-deposition current potential of detector is-400～400mV, the enrichment electrodeposition time is 10～60s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 5～20mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.The vitamin B1 sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.1～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～8mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B2 content, the enrichment electro-deposition current potential of detector is-1500～-500mV, the enrichment electrodeposition time is 20～80s, initial potential is-1500～-500mV, the termination current potential is-400～900mV, the current potential increment is 2～15mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.The vitamin B2 sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is that one or more are formed in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, and the addition of perchlorate is 0.05～10mol/L; Strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～2mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin B6 content, the enrichment electro-deposition current potential of detector is-600～400mV, the enrichment electrodeposition time is 10～60s, initial potential is-600～400mV, the termination current potential is 500～1000mV, the current potential increment is 2～15mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.The vitamin B6 sample treatment solution is made up of phosphate buffered solution, alkaline solution, surfactant; Phosphate buffered solution is by sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, phosphoric acid one hydrogen iron, primary iron phosphate, diammonium hydrogen phosphate, ammonium dihydrogen phosphate (ADP), one or more compositions in the calcium dihydrogen phosphate, the phosphate buffered solution addition is 0.005～10mol/L, alkaline solution is by NaOH, potassium hydroxide, calcium hydroxide, one or more compositions in the ammoniacal liquor, the alkaline solution addition is 0.01～1mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions of polysorbate, the fatty acid sorb is smooth, polysorbate, glycerin monostearate be surfactant most preferably, the surfactant addition is 0.05～10mmol/L.
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample Cobastab 9 content, the enrichment electro-deposition current potential of detector is-2000～-500mV, the enrichment electrodeposition time is 20～80s, initial potential is-2000～-1000mV, the termination current potential is-400～1000mV, the current potential increment is 2～15mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.Cobastab 9 sample treatment solutions are made up of tartrate, acetate buffer solution, surfactant, ionic strength adjustor, strong acid and dimethylglyoxime ethanolic solution; Tartrate is by sodium potassium tartrate tetrahydrate, potassium antimony tartrate, tartrate ammonia potassium, sodium tartrate, one or more compositions in the potassium tartrate, potassium tartrate, sodium potassium tartrate tetrahydrate be tartrate most preferably, the tartrate addition is 2～10mmol/L, acetate buffer solution is by sodium acetate, potassium acetate, one or more are formed with acetic acid in the ammonium acetate, the acetate buffer solution addition is 0.01～10mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions in the polysorbate, cetyl trimethyl ammonium bromide, glycerin monostearate be surfactant most preferably, the surfactant addition is 0.05～50mmol/L, ionic strength adjustor is by potassium chloride, sodium chloride, sodium sulphate, potassium chloride, one or more compositions in the potassium nitrate, the ionic strength adjustor addition is 0.01～10mol/L, strong acid is by hydrochloric acid, sulfuric acid, nitric acid, one or more compositions in the perchloric acid, sodium chloride, potassium chloride, potassium nitrate be ionic strength adjustor most preferably, the strong acid addition is 0.01～5mol/L, and the addition of dimethylglyoxime is 0.01～10mmol/L.
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample cobalamin content, the enrichment electro-deposition current potential of detector is-2000～-500mV, the enrichment electrodeposition time is 20～80s, initial potential is-2000～-1000mV, the termination current potential is-400～1000mV, the current potential increment is 2～15mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.The cobalamin sample treatment solution is made up of tartrate, acetate buffer solution, surfactant, ionic strength adjustor, strong acid, ethanol and dimethylglyoxime; Tartrate is by sodium potassium tartrate tetrahydrate, potassium antimony tartrate, tartrate ammonia potassium, sodium tartrate, one or more compositions in the potassium tartrate, the tartrate addition is 0.02～5mol/L, acetate buffer solution is by by sodium acetate, potassium acetate, one or more are formed with acetic acid in the ammonium acetate, the acetate buffer solution addition is 0.01～10mol/L, surfactant is by stearic acid, neopelex, cetyl trimethyl ammonium bromide, glycerin monostearate, the fatty acid sorb is smooth, one or more compositions in the polysorbate, the surfactant addition is 0.02～10mmol/L, ionic strength adjustor is by potassium chloride, sodium chloride, sodium sulphate, potassium chloride, one or more compositions in the potassium nitrate, the ionic strength adjustor addition is 0.05～10mol/L, strong acid is by hydrochloric acid, sulfuric acid, nitric acid, one or more compositions in the perchloric acid, the strong acid addition is 0.01～5mol/L, and the addition of dimethylglyoxime is 0.02～10mmol/L.
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample Vitamin C content, the enrichment electro-deposition current potential of detector is-400～400mV, the enrichment electrodeposition time is 10～60s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 5～20mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.The vitamin C sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.01～10mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.01～10mol/L; The addition of dimethyl formamide is 0.01～10mmol/L.
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin D content, the enrichment electro-deposition current potential of detector is-200～400mV, the enrichment electrodeposition time is 10～90s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 2～15mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.The vitamin D sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.02～10mol/L; The addition of dimethyl formamide is 0.05～10mmol/L;
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample content of vitamin E, the enrichment electro-deposition current potential of detector is-200～400mV, the enrichment electrodeposition time is 10～90s, initial potential is-200～400mV, the termination current potential is 1000～1800mV, the current potential increment is 2～15mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.The vitamin E sample treatment solution is made up of perchlorate, strong acid, dimethyl formamide; Perchlorate is one or more compositions in sodium perchlorate, potassium perchlorate, ammonia perchlorate, lithium perchlorate, magnesium perchlorate, the silver perchlorate, the perchlorate addition is 0.04～8mol/L, strong acid is by one or more are formed in hydrochloric acid, sulfuric acid, nitric acid, the perchloric acid, and the addition of strong acid is 0.05～10mol/L; The addition of dimethyl formamide is 0.01～2mmol/L.
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample vitamin K1 content, the enrichment electro-deposition current potential of detector is 100～1000mV, the enrichment electrodeposition time is 20～80s, initial potential is-1000～100mV, the termination current potential is-100～1500mV, the current potential increment is 2～15mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.The vitamin K1 sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, ammonium chloride, hydrochloric acid, tetramethyl ammonium chloride be the vitamin K1 sample treatment solution most preferably, addition is 0.02～15mol/L;
The conventional pulse leaching of described detection blood sample vitamin content, be characterized in: when detecting in the blood sample prokeyvit content, the enrichment electro-deposition current potential of detector is 100～1000mV, the enrichment electrodeposition time is 20～80s, initial potential is-1000～100mV, the termination current potential is-100～1500mV, the current potential increment is 2～15mV, pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, and pulse width is 0.01～0.05s, be 10～60s rest time, resting potential is-200～600mV, and the stop time is 10～60s, and range (sensitivity) is 1～500 μ A.The prokeyvit sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, ammonium chloride, hydrochloric acid, tetramethyl ammonium chloride be the prokeyvit sample treatment solution most preferably, addition is 0.01～5mol/L.
Advantage and good effect that the present invention has are:
Detect the conventional pulse leaching of blood sample vitamin content owing to adopted brand-new technology scheme of the present invention, compared with prior art, that the present invention has is highly sensitive, accuracy good, easy and simple to handle, quick, applied range, be suitable for medical department and use, can carry out advantages such as analyzing and testing to the vitamin A in the blood sample, B1, B2, B6, B9, B12, C, D, E, K1, K3.
A kind of method that detects vitamin content in the blood sample provided by the invention has short, advantage such as accuracy is high, sensing range is wide of test duration, can be used for the fast detecting of blood sample vitamin content.
Description of drawings
Fig. 1 is prokeyvit standard model current value of the present invention-concentration standard curve synoptic diagram.
Embodiment
For further understanding technology contents of the present invention, characteristics and effect, exemplify following examples now, and conjunction with figs. is described in detail as follows:
Consult accompanying drawing 1.
Embodiment 1
Detect the conventional pulse leaching of blood sample vitamin content: get a certain amount of testing sample and mix with a certain amount of homemade vitamin sample treating fluid, utilize conventional pulse leaching to make the vitamin in the testing sample at homemade sensor probe oxidation (reduction) reaction take place, produce current signal.By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the testing sample.
Detect the specific implementation process of prokeyvit content in the blood sample:
1. prepare the prokeyvit sample treatment solution: the prokeyvit sample treatment solution is made up of ammonium chloride, hydrochloric acid, tetramethyl ammonium chloride, and addition is 4mol/L.
2. debugging detector: when detecting in the blood sample prokeyvit content, the enrichment electro-deposition current potential of detector is 500mV, and the enrichment electrodeposition time is 40s, initial potential is 50mV, the termination current potential is 100mV, and the current potential increment is 8mV, and pulse width is 0.06s, recurrence interval is 0.2s, pulse width is 0.03s, and be 30s rest time, and resting potential is 200mV, stop time is 40s, and range (sensitivity) is 1～500 μ A.The prokeyvit sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, ammonium chloride, hydrochloric acid, tetramethyl ammonium chloride be the prokeyvit sample treatment solution most preferably, addition is 0.01～5mol/L.
3, prokeyvit standard model-concentration standard curve
(1) accurately pipettes 2000uL prokeyvit sample treatment solution with pipettor;
(2) accurately pipette 10uL, 30uL, 50uL, 70uL, 90uL, 110uL prokeyvit standard model respectively to the prokeyvit sample treatment solution with pipettor, mix;
(3) work for the treatment of electrode and auxiliary electrode under the condition of the prokeyvit detected parameters that has configured, detect the record current signal value respectively;
(4) draw prokeyvit standard model current value-concentration standard curve (accompanying drawing).
4, the method for quick of prokeyvit in the blood sample
(1) accurately pipettes 2000uL prokeyvit sample treatment solution with pipettor;
(2) accurately pipette the 20uL blood sample to the prokeyvit sample treatment solution with pipettor, mix;
(3) work for the treatment of electrode and auxiliary electrode under the condition of the prokeyvit detected parameters that has configured, detect the record current signal value;
(4) compare with typical curve, obtain the content of prokeyvit in the blood sample.
Claims (1)
1. conventional pulse leaching that detects the blood sample vitamin content, it is characterized in that: get blood sample to be measured and mix with vitamin sample treating fluid, the current signal that utilizes conventional pulse leaching test sample when redox reaction takes place vitamin detector sensor probe, to produce; By comparing the current signal value of variable concentrations vitamin standard model, formulate typical curve, according to the current signal value that testing sample produces, obtain the content of vitamin in the blood sample to be measured;
When detecting in the blood sample prokeyvit content, the enrichment electro-deposition current potential of detector is 100～1000mV, and the enrichment electrodeposition time is 20～80s, and initial potential is-1000～100mV, the termination current potential is-100～1500mV, the current potential increment is 2～15mV, and pulse width is 0.04～0.08s, and the recurrence interval is 0.1～0.3s, pulse width is 0.01～0.05s, be 10～60s rest time, and resting potential is-200～600mV, and the stop time is 10～60s; The prokeyvit sample treatment solution is made up of in ammonium chloride, ammonium nitrate, ammoniacal liquor, hydrochloric acid, tetramethyl ammonium chloride, diethylamine, the triethylamine one or more, and addition is 0.01～5mol/L.
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