CN102071140A - Composite microbial biogen capable of degrading organic waste efficiently and preparation method and applications thereof - Google Patents
Composite microbial biogen capable of degrading organic waste efficiently and preparation method and applications thereof Download PDFInfo
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Abstract
The invention provides a composite microbial biogen capable of degrading organic waste efficiently and a preparation method and applications thereof. The composite microbial biogen contains Bacillus circulans, Bacillus subtilis, Alcaligenes faecalis and Penicillium diversum. The composite microbial biogen can be used with activated sludge for the existing sewage treatment equipment; under the synergetic effect, the yield of excess sludge can be greatly reduced, the aims of emission reduction and energy saving can be realized and the sludge problem which is difficult to overcome for a long time in sludge treatment is effectively solved; and the biogen has importanct economic importance and environmental importance and is suitable for each sewage treatment plant. The composite microbial biogen can also be used to prepare organic wastewater purifying agents, activated sludge enhancers of urban domestic wastewater and deodorants used in tipping sites, public places, agricultural product market, kitchens, bathrooms and the like.
Description
Technical field
The invention belongs to the microbial environment protecting field, composite microorganism viable bacteria preparation of particularly a kind of efficient degradation organic waste and its production and application.
Background technology
Along with people's constantly popularizing to water resources protection understanding; various places are built enlarging sewage work energetically; treatment rate of domestic sewage improves year by year; though adopt the general COD energy of traditional sewage treatment process up to standard; but the ammonia nitrogen treatment effect is relatively poor, exists surplus sludge volume big, shortcomings such as processing cost height; method for sludge treatment commonly used mainly is landfill, burning and agricultural, will cause secondary pollution to environment but deal with improperly.The random outward transport landfill of wet mud has caused mud to surround the city, and the objectionable impurities in the mud is with rain erosion and leak out to underground water and cause second environmental pollution; Burning needs wet mud is carried out mummification, process cost height, and exhaust gas discharged can cause secondary pollution to environment as dealing with improperly; It is agricultural that heavy metal in the wet mud, harmful bacterium, parasite and the foul smell that distributes have all seriously restricted mud.Therefore the decrement discharge of realizing mud in sewage treatment process has more and more caused each side's concern.
(contriver Wang He is upright in Chinese invention patent application 200710130220.6, denomination of invention: the high-efficiency composite hydrolysis acidification pool that has sewage disposal and sludge reduction functions concurrently) method by hydrolysis acidification pool is set before the sewage aeration pond is to the returned sluge liquefaction that is hydrolyzed, raising realizes the decrement discharge of mud to the clearance of organic pollutant.
Chinese invention patent application 200810029836.9 (contriver's beam fortune is auspicious etc., denomination of invention: a kind of microorganism water purifying assistant and preparation method thereof and application) obtains a series of enzymes such as cellulase, zytase, amylase, proteolytic enzyme and lipase and make microbial water-purifying agent by fermentation, drying, pulverizing aspergillus niger thalline, reach the effect that purifies water by the organism in the enzymic hydrolysis sewage.
But these method processing costs are higher, and efficient is low, is still restricting properly settling of mud problem.
Summary of the invention
Therefore, the technical problem to be solved in the present invention is exactly to have the big defective of mud discharge capacity at existing sewage disposal technology, composite microorganism viable bacteria preparation of a kind of efficient degradation organic waste and its production and application is provided, this composite microorganism viable bacteria preparation can decompose organic waste rapidly, effectively suppress the harmful microorganism growth, make the suspended substance flocculation in the sewage, effectively improve sewage colourity, eliminate stink, make that the discharge capacity of mud reduces greatly in the sewage disposal.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of composite microorganism viable bacteria preparation of efficient degradation organic waste, contain Bacillus circulans (Bacillus circulans), subtilis (Bacillus subtilis), Alcaligenes faecalis (Alcaligenes faecalis) and heteroplasia mould (Penicillium diversum).
Among the present invention, described Bacillus circulans, subtilis, Alcaligenes faecalis and heteroplasia mould all are the existing conventional bacterial classifications, such as obtaining from Chinese common micro-organisms culture presevation administrative center (CGMCC) and American Type Culture Collecti (ATCC) purchase.
Wherein, described Bacillus circulans is the bacterium that belongs to Bacillus, and is preferable for being selected among Bacillus circulans (Bacillus circulans) CGMCC 1.554, CGMCC 1.383, ATCC 13403, ATCC 15518 and the ATCC 21656 one or more.The bacterium number of described Bacillus circulans account for the per-cent of total count preferable be 40～60%.The secreted ablastins of Bacillus circulans can produce functional disturbance to the cytoplasmic membrane of bacterium, can kill the deleterious pathogenic agent to people and animals, simultaneously Gram-negative and gram-positive microorganism are had antibiotic effect.And the sheet coccus is an amphimicrobe, can decompose putrid organic substance in stratified water surrounding.
Wherein, described subtilis is the bacterium that belongs to Bacillus, and is preferable for being selected among subtilis (Bacillus subtilis) CGMCC 1.308, CGMCC 1.836, CGMCC 1.933, ATCC 10783, ATCC 13472 and the ATCC 13953 one or more.The bacterium number of described subtilis account for the per-cent of total count preferable be 10～35%.Subtilis can effectively be decomposed various carbohydrate, sugar, starch and range protein amino acid, for other effective microbes provide propagation required nutritive substance.Subtilis can secrete multiple enzyme, as cellulase, hemicellulase, zytase, amylase, polygalacturonase and ligninase etc., the more effectively organic substance in the degradation water.
Wherein, described Alcaligenes faecalis is the bacterium that belongs to Alkaligenes, and is preferable for being selected among Alcaligenes faecalis (Alcaligenes faecalis) CGMCC 1.924, CGMCC 1.1799 and the CGMCC 1.2006 one or more.The bacterium number of described Alcaligenes faecalis account for the per-cent of total count preferable be 5～15%.Alcaligenes faecalis can be secreted multiple enzyme, and as proteolytic enzyme, peptase, lipase etc., effectively organism in the water of decomposition is eliminated peculiar smell.
Wherein, described heteroplasia mould is the filamentous fungus bacterial classification that belongs to Penicillium, and is preferable for being selected among heteroplasia green grass or young crops (Penicillium diversum) ATCC 10437, ATCC 52210, the ATCC 66706 one or more.The bacterium number of described heteroplasia mould account for the per-cent of total count preferable be 5～15%.The heteroplasia mould can be secreted multiple polysaccharose substance etc., particulate matter can be assembled together, is convenient to decompose.
In the composite microorganism viable bacteria preparation of the present invention, what the total count of mentioned microorganism was preferable is more than 1,000,000,000/gram.
In the composite microorganism viable bacteria preparation of the present invention, can such as the used matrix of various solid fermentations, comprise ground rice, rice bran, wheat bran, one or more of dregs of beans etc. etc. with the auxiliary material of this area routine as carrier.Preferable prescription is: 20% ground rice, rice bran 60%, wheat bran 10%, and dregs of beans 10%, trace element solution 1%, per-cent are volume percent; Described trace element solution is a sal epsom 10%, and KCl 10%, and water 80%, per-cent are percents weight in volume.
There is good synergy between the microorganism that composite microorganism viable bacteria preparation of the present invention contains.Under this synergy, effectively decompose a large amount of spoilage organism matter, suppress the harmful microorganism growth, reduce active sludge and grow fast, eliminate stink.Wherein, Alcaligenes faecalis, Bacillus circulans and subtilis utilize the organic waste growth, secrete multiple enzyme simultaneously, can decompose various larger molecular organicses effectively, as Mierocrystalline cellulose, and starch, protein, greases etc. are eliminated peculiar smell.The secondary metabolite antibacterial peptide that Bacillus circulans and subtilis produced can effectively suppress the harmful microbe breeding, kills the deleterious pathogenic agent to people and animals.The heteroplasia mould then utilizes organic substance to breed under aerobic condition through decomposing the various nutritive substances that produce.The heteroplasia mould can effectively decompose various carbohydrate, sugar, starch and range protein amino acid, for other effective microbes provide propagation required nutritive substance; Secreted multiple enzyme, as cellulase, hemicellulase, zytase, amylase, polygalacturonase and ligninase etc., the organic substance in the degradation water more effectively, the polysaccharide that it produced simultaneously can make suspended substance flocculation in the water, have effectively improved sewage colourity.
The preparation method of composite microorganism viable bacteria preparation of the present invention can be undertaken by this area ordinary method, respectively Bacillus circulans (Bacillus circulans), subtilis (Bacillussubtilis), Alcaligenes faecalis (Alcaligenes faecalis) and heteroplasia mould (Penicillium diversum) are amplified liquid culture, then by required mixed, solid fermentation, dry, be ground into powder, can make.Preferable, may further comprise the steps:
(1) carries out amplification culture respectively
Bacillus circulans after slant activation, is inoculated in the liquid nutrient medium, cultivated 44～52 hours for 35～37 ℃;
Subtilis after slant activation, is inoculated in the liquid nutrient medium, cultivated 44～52 hours for 28～37 ℃;
Alcaligenes faecalis after slant activation, is inoculated in the liquid nutrient medium, cultivated 44～52 hours for 27～31 ℃;
The heteroplasia mould after slant activation, is inoculated in the liquid nutrient medium, cultivated 48～96 hours for 25～30 ℃;
Live bacterial count mixes bacterium liquid according to required ratio;
(2) 28～35 ℃ of solid fermentations 48～72 hours, 40～60 ℃ of oven dry are crushed to 15～50 orders, sieve.
In the step (1), described bacterium liquid mixes used ratio, the bacterium number of Bacillus circulans account for the per-cent of total count preferable be 40～60%; The bacterium number of subtilis account for the per-cent of total count preferable be 10～35%; The bacterium number of Alcaligenes faecalis account for the per-cent of total count preferable be 5～15%; The bacterium number of heteroplasia mould account for the per-cent of total count preferable be 5～15%.
In step (1) and (2), the operation of described liquid culture and solid fermentation is by this area routine operation, and wherein liquid nutrient medium and solid fermentation matrix adopt this area common prescription.
Wherein, the liquid culture based formulas of described Bacillus circulans preferably: glucose 3%, tryptone 1.5%, extractum carnis 3%, peptone 2%, tween 80 0.1%, sodium acetate 0.5%, ammonium citrate 0.2%, sal epsom 0.058%, manganous sulfate 0.25%, pH6.5, per-cent is percent weight in volume, and promptly gram is/100 milliliters.
The liquid culture based formulas of described subtilis is preferably: soyflour 1.5%, and starch 1%, yeast extract paste 0.2%, glucose 1%, pH6.5, per-cent are percents weight in volume.
Described Alcaligenes faecalis can adopt identical liquid nutrient medium with the heteroplasia mould, this liquid culture based formulas preferably: glucose 1%, malt meal 0.2%, yeast extract paste 0.2%, sodium acetate 0.5%, pH7.0, per-cent are percents weight in volume.
The prescription of described solid fermentation matrix is preferably: 20% ground rice, rice bran 60%, wheat bran 10%, and dregs of beans 10%, trace element solution 1%, per-cent are volume percent; Described trace element solution is a sal epsom 10%, and KCl 10%, and water 80%, per-cent are percents weight in volume.
Composite microorganism viable bacteria preparation of the present invention is mainly used in the existing sewage treatment process.Existing sewage treatment process comprises and utilizes active sludge to carry out sewage disposal, composite bacteria preparation of the present invention and active sludge can be united use, the input ratio (weight ratio) of composite bacteria preparation and active sludge is, 1: 1000～1: 10000, and preferably 1: 2000.Throw in preferably 0.1～50 gram of composite microorganism viable bacteria preparation in every cubic metre of sewage, more preferably 0.4～20 gram.The excess sludge discharge amount can be reduced effectively, also effluent quality can be further improved.Can also be used to prepare the organic waste water water purification agent, the active sludge reinforcer of urban domestic wastewater.
Composite microorganism viable bacteria preparation of the present invention such as also can be used as between refuse tip, public place, market for farm products and Kitchen hygiene at the reodorant in place.With spraying behind the composite microorganism viable bacteria preparation dilute with water, get final product deodorizing, what the sprinkling consumption was preferable is 100 milliliter of 1% (w/v) composite microorganism viable bacteria preparation aqueous solution of every square metre of sprinkling.
Among the present invention, but above-mentioned optimum condition arbitrary combination on the basis that meets this area general knowledge promptly gets the preferred embodiments of the invention.
The present invention is except that specifying, used per-cent all is mass percent.
Raw material that the present invention is used or reagent except that specifying, all commercially available getting.
Than prior art, beneficial effect of the present invention is as follows: the invention provides a kind of complex microorganism preparations, it can be applied in the existing sewage treatment process, is fit to each sewage work and uses.In use, can use existing equipment, throw in active sludge, under synergy, reduce the excess sludge growing amount greatly, reach reduction of discharging and energy conservation object, efficiently solve the mud problem that being difficult to for a long time in the sewage disposal overcomes, have great economic implications and environment protection significance.And this strain preparation is convenient, and is with low cost, after more using the cost of sludge treatment descended greatly, is the key breakthrough of sludge treatment problem.
Embodiment
Further specify the present invention with embodiment below, but the present invention is not limited.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.Among the embodiment, used bacterial classification is all buied from the Chinese common micro-organisms culture presevation administrative center or U.S. representative microbial preservation center (ATCC).
Embodiment 1
(1) carry out amplification culture respectively:
CGMCC 1.554 is inoculated in the liquid nutrient medium after slant activation with Bacillus circulans (Bacillus circulans), cultivates 48 hours for 35 ℃.Liquid culture based formulas: glucose 3%, tryptone 1.5%, extractum carnis 3%, peptone 2%, tween 80 0.1%, sodium acetate 0.5%, ammonium citrate 0.2%, sal epsom 0.058%, manganous sulfate 0.25%, pH6.5, per-cent are percents weight in volume, promptly gram is/100 milliliters.
With subtilis (Bacillus subtilis) CGMCC 1.308 with after slant activation, be inoculated in the liquid nutrient medium, cultivated 48 hours for 28 ℃.The liquid culture based formulas: soyflour 1.5%, starch 1%, yeast extract paste 0.2%, glucose 1%, pH6.5, per-cent are percents weight in volume.
Alcaligenes faecalis (Alcaligenes faecalis) CGMCC 1.924 and heteroplasia mould (Penicilliumdiversum) ATCC 10437 after slant activation, are inoculated in respectively in the liquid nutrient medium, cultivated 48 hours for 30 ℃.The liquid culture based formulas: glucose 1%, malt meal 0.2%, yeast extract paste 0.2%, sodium acetate 0.5%, pH7.0, per-cent are percents weight in volume.
Live bacterial count detects the bacterium number, and the per-cent that accounts for total count according to the bacterium numbers of following various bacterium mixes: Bacillus circulans 60%, withered grass gemma 25%, Alcaligenes faecalis 10%, heteroplasia mould 5%.Total viable count is higher than 1,500,000,000/milliliter fermentation broth sample.
(2) (trace element solution 1%, per-cent are volume percent for 20% ground rice, rice bran 60%, wheat bran 10%, dregs of beans 10% to add solid fermentation matrix in the solid-state fermentation tank; Described trace element solution is a sal epsom 10%, and KCl 10%, and water 80%, per-cent are percents weight in volume).121 ℃ of sterilizations 30 minutes are treated to spray into mixed bacteria liquid (adding 1.5 kilograms of solid basestocks according to 1 liter of fermented liquid) below jar temperature drop to 40 ℃ under the situation of fermentor tank rotation, 28 ℃ of solid fermentations 48 hours, and 40 ℃ of oven dry are pulverized, and sieve through 20 orders, and are standby.Live bacterial count, total count reach more than 1,000,000,000/gram, the packing pack.
Embodiment 2
(1) carry out amplification culture respectively:
Bacillus circulans (Bacillus circulans) CGMCC 1.383, ATCC 13403, ATCC15518 and ATCC 21656 after slant activation, are inoculated in the liquid nutrient medium, cultivated 44 hours for 37 ℃.The liquid culture based formulas is with embodiment 1.
With subtilis (Bacillus subtilis), CGMCC 1.836, CGMCC 1.933, ATCC10783, ATCC 13472 and ATCC 13953 with after slant activation, be inoculated in the liquid nutrient medium, cultivated 44 hours for 37 ℃.The liquid culture based formulas is with embodiment 1.
Alcaligenes faecalis (Alcaligenes faecalis) CGMCC 1.1799 and CGMCC 1.2006 after slant activation, are inoculated in the liquid nutrient medium, cultivated 52 hours for 27 ℃.The liquid culture based formulas is with embodiment 1.
Heteroplasia mould (Penicillium diversum) ATCC 52210, ATCC 66706 after slant activation, are inoculated in the liquid nutrient medium, cultivated 96 hours for 25 ℃.The liquid culture based formulas is with embodiment 1.
Live bacterial count detects the bacterium number, and the per-cent that accounts for total count according to the bacterium numbers of following various bacterium mixes: Bacillus circulans 40%, withered grass gemma 35%, Alcaligenes faecalis 15%, heteroplasia mould 10%.Bacterium is counted sum and is higher than 1,500,000,000/milliliter fermentation broth sample.
(2) add solid fermentation matrix (with embodiment 1) in the solid-state fermentation tank, with spraying into mixed bacteria liquid after embodiment 1 sterilization.30 ℃ of solid fermentations 72 hours, 60 ℃ of oven dry are pulverized, and sieve through 50 orders, and are standby.Live bacterial count, total count reach more than 1,000,000,000/gram, the packing pack.
Embodiment 3
(1) carry out amplification culture respectively:
ATCC 13403 is inoculated in the liquid nutrient medium after slant activation with Bacillus circulans (Bacillus circulans), cultivates 50 hours for 36 ℃.The liquid culture based formulas is with embodiment 1.
With subtilis (Bacillus subtilis) CGMCC 1.933 with after slant activation, be inoculated in the liquid nutrient medium, cultivated 50 hours for 30 ℃.The liquid culture based formulas is with embodiment 1.
CGMCC 1.2006 is inoculated in the liquid nutrient medium after slant activation with Alcaligenes faecalis (Alcaligenes faecalis), cultivates 44 hours for 31 ℃.The liquid culture based formulas is with embodiment 1.
ATCC 66706 is inoculated in the liquid nutrient medium after slant activation with heteroplasia mould (Penicillium diversum), cultivates 72 hours for 29 ℃.The liquid culture based formulas is with embodiment 1.
Live bacterial count detects the bacterium number, and the per-cent that accounts for total count according to the bacterium numbers of following various bacterium mixes: Bacillus circulans 60%, withered grass gemma 10%, Alcaligenes faecalis 15%, heteroplasia mould 15%.Bacterium is counted sum and is higher than 1,500,000,000/milliliter fermentation broth sample.
(2) add solid fermentation matrix (with embodiment 1) in the solid-state fermentation tank, with spraying into mixed bacteria liquid after embodiment 1 sterilization.33 ℃ of solid fermentations 60 hours, 50 ℃ of oven dry are pulverized, and sieve through 30 orders, and are standby.Live bacterial count, total count reach more than 1,000,000,000/gram, the packing pack.
Embodiment 4
(1) carry out amplification culture respectively:
ATCC 15518 is inoculated in the liquid nutrient medium after slant activation with Bacillus circulans (Bacillus circulans), cultivates 48 hours for 35 ℃.The liquid culture based formulas is with embodiment 1.
With subtilis (Bacillus subtilis) ATCC 10783 with after slant activation, be inoculated in the liquid nutrient medium, cultivated 48 hours for 33 ℃.The liquid culture based formulas is with embodiment 1.
Alcaligenes faecalis (Alcaligenes faecalis) CGMCC 1.2006 and heteroplasia mould (Penicilliumdiversum) ATCC 66706 after slant activation, are inoculated in respectively in the liquid nutrient medium, cultivated 48 hours for 30 ℃.The liquid culture based formulas is with embodiment 1.
Live bacterial count detects the bacterium number, and the per-cent that accounts for total count according to the bacterium numbers of following various bacterium mixes: Bacillus circulans 55%, withered grass gemma 30%, Alcaligenes faecalis 10%, heteroplasia mould 5%.Bacterium is counted sum and is higher than 1,500,000,000/milliliter fermentation broth sample.
(2) add solid fermentation matrix (with embodiment 1) in the solid-state fermentation tank, with spraying into mixed bacteria liquid after embodiment 1 sterilization.28 ℃ of solid fermentations 72 hours, 55 ℃ of oven dry are pulverized, and sieve through 15 orders, and are standby.Live bacterial count, total count reach more than 1,000,000,000/gram, the packing pack.
Embodiment 5
(1) carry out amplification culture respectively:
ATCC 21656 is inoculated in the liquid nutrient medium after slant activation with Bacillus circulans (Bacillus circulans), cultivates 48 hours for 35 ℃.The liquid culture based formulas is with embodiment 1.
With subtilis (Bacillus subtilis) ATCC 13953 with after slant activation, be inoculated in the liquid nutrient medium, cultivated 48 hours for 28 ℃.The liquid culture based formulas is with embodiment 1.
Alcaligenes faecalis (Alcaligenes faecalis) CGMCC 1.2006 and heteroplasia mould (Penicilliumdiversum) ATCC 66706 after slant activation, are inoculated in respectively in the liquid nutrient medium, cultivated 48 hours for 30 ℃.The liquid culture based formulas is with embodiment 1.
Live bacterial count detects the bacterium number, and the per-cent that accounts for total count according to the bacterium numbers of following various bacterium mixes: Bacillus circulans 45%, withered grass gemma 35%, Alcaligenes faecalis 5%, heteroplasia mould 15%.Bacterium is counted sum and is higher than 1,500,000,000/milliliter fermentation broth sample.
(2) add solid fermentation matrix (with embodiment 1) in the solid-state fermentation tank, with spraying into mixed bacteria liquid after embodiment 1 sterilization.25 ℃ of solid fermentations 60 hours, 45 ℃ of oven dry are pulverized, and sieve through 40 orders, and are standby.Live bacterial count, total count reach more than 1,000,000,000/gram, the packing pack.
Further specify beneficial effect of the present invention below by effect embodiment.
Effect embodiment 1
City domestic sewage, aeration tank useful volume are 100L, temperature 15～20 degree, drop into composite microorganism viable bacteria preparation 2.0 grams (powder) (embodiment 1 preparation), flooding quantity 5L/ hour, COD was 1000～1200mg/L, ammonia nitrogen is 50～80mg/L, and dissolved oxygen is controlled at 3～4mg/L.Each adds active sludge 5L, moves 5 days continuously, the results are shown in Table 1.
Table 1. city domestic sewage is handled the results of comparison in the aeration tank
| Aerobe technology | COD average removal rate % | Ammonia nitrogen average removal rate % | Surplus sludge volume L |
| Composite bacteria+active sludge | 93.4 | 68.3 | 6L |
| Active sludge | 88.7 | 55.8 | 20L |
As seen, under the identical situation of other condition, the processing COD clearance of the independent use of composite microorganism viable bacteria preparation combined activity mud ratio of the present invention active sludge, ammonia nitrogen removal frank are apparently higher than conventional activated sludge process, significantly reducing active sludge produces, greatly reduced the excess sludge processing cost, economical effectiveness is obvious.
Effect embodiment 2
The water outlet after anaerobism of pig farm sewage, the aeration tank useful volume is 100L, temperature 25～30 degree drop into composite microorganism viable bacteria preparation 2.0 grams (powder) (embodiment 2 preparations), flooding quantity 4L/ hour, COD is 1260～965mg/L, ammonia nitrogen is 178～264mg/L, and dissolved oxygen is controlled at 3～4mg/L, respectively adds active sludge 4L, operation is 5 days continuously, the results are shown in Table 2.
The results of comparison in aeration in sewage treatment pond, table 2. pig farm
| Aerobe technology | COD average removal rate % | Ammonia nitrogen average removal rate % | Colibacillus (individual/mL) | Surplus sludge volume L | Stink |
| Composite bacteria+active sludge | 90.3 | 74.4 | 120 | 6 | Do not have |
| Active sludge | 82.6 | 58.3 | 5×10 3 | 28 | Have |
As seen, the processing of composite microorganism viable bacteria preparation of the present invention reduces excess sludge in a large number and produce, and the Wastewater Treated by Activated Sludge Process effect descends obviously separately the stronger restraining effect of colibacillus.It is 10 degree that temperature raises, and the treatment effect of composite microorganism viable bacteria preparation of the present invention and active sludge combination is still better.
Effect embodiment 3
Food processing plant's composite waste, pH are 6～9, and flooding quantity 5L/ hour, COD was 460～765mg/L, and ammonia nitrogen is 78～107mg/L, and dissolved oxygen is controlled at 3～4mg/L.The aeration tank useful volume is 100L, and temperature 20 degree drop into composite microorganism viable bacteria preparation 2.0 grams (powder) (embodiment 3 preparations), respectively add active sludge 5L, move 10 days continuously, the results are shown in Table 3.
Table 3. food processing plant composite waste is handled the comparing result of aeration tank
| Aerobe technology | COD average removal rate % | Ammonia nitrogen average removal rate % | Surplus sludge volume L |
| Composite bacteria+active sludge | 96.3 | 71.5 | 7 |
| Active sludge | 89.6 | 52.4 | 35 |
As seen, to food industrial wastewater, the treatment effect of composite microorganism viable bacteria preparation of the present invention and active sludge combination is significantly better than activated sludge process, and excess sludge significantly reduces.
Effect embodiment 4
In the active sludge treatment process of 100,000 tons of municipal wastewater treatment plants, amplify research,, add microbiobacterial agent (embodiment 4 preparations) and be 40 kilograms of every days, water temperature 10～15 degree by day handling about 10 ten thousand steres of the water yield at present.Add the ingress that bacterium point is located at the A/A/O aerobic section.Active sludge is constant, still adopts former technology (former technology is used A/A/O technology, does not add microbiobacterial agent).Select other 100,000 tons of active sludge treatment ponds not add bacterium in contrast simultaneously, every day, the sampling analysis water quality parameter moved 2 wheat harvesting periods continuously, and 8 day data see Table 4.
The amplification result of study of table 4. in active sludge treatment process
Carry out composite bacteria and the discovery of active sludge combination research at municipal wastewater treatment plant Da Chi, residual active sludge significantly reduces, and COD, ammonia nitrogen, nitrogen removal rate are all better.Show that this microbial inoculum is not only optimized water quality with the active sludge synergy, also can significantly reduce the generation of excess sludge.Be amplified to the sewage work of 100,000 tons of scales from laboratory scale, all show good effect.
Effect embodiment 5
Implement on-the-spot deodorizing test in refuse landfill, meteorological conditions be temperature about 25 ℃, wind speed is below 3 grades.Lay a sampling point, test item: ammonia, two projects of hydrogen sulfide at the center, operating area.Background values detects before spraying.Spraying consumption is undertaken by 100 milliliters of reodorant of one square metre of sprinkling; The reodorant that sprays promptly is that the microbial inoculum of embodiment 1 preparation adds 100 milliliters of dissolved dilutions of clear water according to 1 gram microbial inoculum and forms.Spray and detected in back 10 minutes, 40 minutes, the results are shown in Table 5.
Table 5. composite microorganism viable bacteria preparation deodorizing test result
As seen, composite microorganism viable bacteria preparation of the present invention is as reodorant, and for household refuse landfill sites deodorizing instant effect, effect is obvious.
Claims (10)
1. the composite microorganism viable bacteria preparation of efficient degradation organic waste, it is characterized in that it contains Bacillus circulans Bacillus circulans, subtilis Bacillus subtilis, Alcaligenes faecalis Alcaligenes faecalis and heteroplasia mould Penicillium diversum.
2. composite microorganism viable bacteria preparation as claimed in claim 1, it is characterized in that, the per-cent that the bacterium number of described Bacillus circulans accounts for total count is 40～60%, the per-cent that the bacterium number of described subtilis accounts for total count is 10～35%, the per-cent that the bacterium number of described Alcaligenes faecalis accounts for total count is 5～15%, and the per-cent that the bacterium number of described heteroplasia mould accounts for total count is 5～15%.
3. composite microorganism viable bacteria preparation as claimed in claim 1, it is characterized in that, described Bacillus circulans is for being selected from Bacillus circulans Bacillus circulans CGMCC 1.554, CGMCC1.383, ATCC 13403, among ATCC 15518 and the ATCC 21656 one or more, described subtilis is for being selected from subtilis Bacillus subtilis CGMCC 1.308, CGMCC1.836, CGMCC 1.933, ATCC 10783, among ATCC 13472 and the ATCC 13953 one or more, described Alcaligenes faecalis is for being selected from Alcaligenes faecalis Alcaligenes faecalis CGMCC 1.924, among CGMCC 1.1799 and the CGMCC 1.2006 one or more, described heteroplasia mould is for being selected from heteroplasia mould Penicillium diversum ATCC 10437, among ATCC 52210 and the ATCC 66706 one or more.
4. composite microorganism viable bacteria preparation as claimed in claim 1 is characterized in that, in the described composite microorganism viable bacteria preparation, the total count of described microorganism is more than 1,000,000,000/gram.
5. composite microorganism viable bacteria preparation as claimed in claim 1 is characterized in that, described composite microorganism viable bacteria preparation with the auxiliary material of this area routine as carrier.
6. preparation method as each described composite microorganism viable bacteria preparation of claim 1～5, it is characterized in that, comprise respectively Bacillus circulans Bacillus circulans, subtilis Bacillus subtilis, Alcaligenes faecalis Alcaligenes faecalis and heteroplasia mould Penicilliumdiversum are amplified liquid culture, then by required mixed, solid fermentation, drying is ground into powder, promptly.
7. preparation method as claimed in claim 6 is characterized in that, may further comprise the steps:
(1) carries out amplification culture respectively
Bacillus circulans after slant activation, is inoculated in the liquid nutrient medium, cultivated 44～52 hours for 35～37 ℃;
Subtilis after slant activation, is inoculated in the liquid nutrient medium, cultivated 44～52 hours for 28～37 ℃;
Alcaligenes faecalis after slant activation, is inoculated in the liquid nutrient medium, cultivated 44～52 hours for 27～31 ℃;
The heteroplasia mould after slant activation, is inoculated in the liquid nutrient medium, cultivated 48～96 hours for 25～30 ℃;
Live bacterial count mixes bacterium liquid according to required ratio;
(2) 28～35 ℃ of solid fermentations 48～72 hours, 40～60 ℃ of oven dry are crushed to 15～50 orders, sieve.
8. preparation method as claimed in claim 7, it is characterized in that, in the step (1), described bacterium liquid mixes used ratio: the per-cent that the bacterium number of Bacillus circulans accounts for total count is 40～60%, the per-cent that the bacterium number of subtilis accounts for total count is 10～35%, the per-cent that the bacterium number of Alcaligenes faecalis accounts for total count is 5～15%, and the per-cent that the bacterium number of heteroplasia mould accounts for total count is 5～15%.
As each described composite microorganism viable bacteria preparation of claim 1～5 in Application of Sewage.
10. preparing the organic waste water water purification agent as each described composite microorganism viable bacteria preparation of claim 1～5, the active sludge reinforcer of urban domestic wastewater, the application between refuse tip, public place, market for farm products and Kitchen hygiene etc. in the reodorant in place.
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| CN201010133588XA CN102071140B (en) | 2010-03-26 | 2010-03-26 | Composite microbial biogen capable of degrading organic waste efficiently and preparation method and applications thereof |
| HK11110098.7A HK1156342A1 (en) | 2010-03-26 | 2011-09-23 | A composition microbial bacteria formulation for degrading organic pollutant efficiently, methods of preparation, and uses thereof |
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| CN201010133588XA CN102071140B (en) | 2010-03-26 | 2010-03-26 | Composite microbial biogen capable of degrading organic waste efficiently and preparation method and applications thereof |
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| CN102583925A (en) * | 2012-02-03 | 2012-07-18 | 哈尔滨工业大学 | Method for pretreating excess sludge by adopting bio-augmentation technology |
| CN102745821A (en) * | 2012-07-30 | 2012-10-24 | 石家庄开发区德赛化工有限公司 | Compound microorganism bacterium agent used for sludge reduction, preparation method and application thereof |
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| CN102965294A (en) * | 2011-10-25 | 2013-03-13 | 北京农业生物技术研究中心 | Alcaligenes faecalis, method for preparation of desulfurization deodorant from the same and application |
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| Publication number | Publication date |
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| HK1156342A1 (en) | 2012-06-08 |
| CN102071140B (en) | 2012-07-04 |
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