CN101947332A - Porous biodegradable composite-type bone repairing material and preparation method thereof - Google Patents
Porous biodegradable composite-type bone repairing material and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a porous biodegradable composite-type bone repairing material and a preparation method thereof. The porous biodegradable composite bone repairing material is characterized by comprising a biodegradable bone regeneration repairing material which is prepared from bioceramic particles and a biodegradable macromolecule composite and has a communicated pore structure, wherein the average pore diameter of each pore is 440-810 mum. Through vitro experiments and animal experiments, the bone repairing stent material is shown to have favorable biocompatibility, biodegradability and bone conductibility, can quicken new bone minerals and has the following characteristics that: the material can be prepared according to the size and the shape of a bone defect, the rough surface of the material is easy for adhesion growth of osteoblasts, high porosity of the stent material and the continuity of the pores are easy for cells to be quickly proliferated and dispersed inside the material, and the bone remodeling speed is high and the vivo degradation speed of the material is equivalent to a bone forming speed.
Description
Technical field
The invention belongs to a kind of osteanagenesis repair materials, particularly relate to a kind of compound bone renovating material and manufacture method of perforated biodegradable.
Background technology
The osteanagenesis repair materials is mainly used in each medical field such as Orthopeadic Surgery, plastic surgery and the department of stomatology at present, because of the damaged osteanagenesis repairing and treating of bone of a variety of causes formation.For example: Orthopeadic Surgery, repair because of bone tumor or osteomyelitis lesion resection postoperative osseous tissue defective region, the osseous tissue defective region after the comminuted fracture; Plastic surgery and oral cavity orthognathic surgery, the bone amount when filling up the bone form and moulding is incomplete; The tooth-planting surgery is implanted needs in order to satisfy implantation body, repairs the jawbone bone quantity not sufficient zone that forms because of a variety of causes in advance; Oral and Maxillofacial Surgery is repaired cleft palate patient's palate crack etc.And the bone tissue restoration inorganic material of clinical widespread usage---tricalcium phosphate is a bioactive ceramics, and excellent biological compatibility is arranged, and can directly combine with osseous tissue, and has the bone regeneration capability of inducing (people such as Kondo N, Biomaterials2005,26,5600-08); But its moulding difficulty, can't satisfy the filling needs of complicated clinically bone defect area, and the hard block material biodegradation rate that casting goes out is slow, osseous tissue is continued regeneration causes occupancy to hinder, and body fluid and preosteoblast also can't be immersed in material internal and break up, breed, can't finish area of new bone in a short time and substitute, the bone that extended is repaired the course of treatment.Ideal to go out the initial stage stable because can't obtain in the direct embedded material of implantation body, and can't form biological the combination with material, need to wait for lacking the bone district, to be substituted the rear by enough area of new bone implantable, thus the block bioactive ceramics of single application can prolong implantation body's implant surgery the waiting period.
Biodegradable macromolecules such as the polymer of the another kind of tissue repair organic material of clinical practice---lactic acid, glycolic or copolymer have excellent biological compatibility, and plasticity is strong, and vivo degradation is rapid; But machinery intensity decreases during its degradation in vivo, the catabolite of generation be acid, can make to organize acid-base value decline around the material, promptly influence osteanagenesis (people such as LiWJ, Acta biomaterialia, 2006,2,377-85).
Summary of the invention
The present invention provides a kind of advantage that not only has two kinds of materials of described prior art for solving the technical problem that exists in the known technology, and on form, can satisfy the filling needs of complicated clinically bone defect area, and can finish compound bone renovating material and manufacture method that area of new bone substitutes the perforated biodegradable made from bioactive ceramics particle and the composite novel complexes of biodegradation high molecular in a short time.
The technical scheme that the present invention takes for the compound bone renovating material of the made perforated biodegradable of the technical problem that exists in the solution known technology is: it includes by the complex of bioactive ceramics particle and biodegradation high molecular and makes, have air hole structure, the biodegradable osteanagenesis repair materials of connection, the average pore size of described connection pore is 440-810 μ m.
Repair materials of the present invention can also adopt following technical scheme:
Described bioactive ceramics particle is the bioactive ceramics with osteoinductive---(β-TCP), density is 3.14g/cm to bata-tricalcium phosphate
3, be Powdered.
Described biodegradation high molecular is the biodegradation high molecular of possess hydrophilic property---polyglycolic acid (PGA), density are 1.53g/cm
3
The technical scheme that the present invention takes for the manufacture method of the compound osteanagenesis repair materials of the made perforated biodegradable of the technical problem that exists in the solution known technology is: fully be dissolved in biodegradation high molecular polyglycolic acid (PGA) in the organic solvent, add powdered biological active ceramic particle bata-tricalcium phosphate (β-TCP) again, fully behind the concussion mixing, with the prefabricated model of the common injection of pore occupy-place particle, through condensing, the solvent volatilization, the filtering of pore occupy-place particle, sterilization, dry, final obtain that (β-TCP) and biodegradation high molecular polyglycolic acid (PGA) complex make, and has to be communicated with air hole structure with bioactive ceramics particle bata-tricalcium phosphate, porous, biodegradable osteanagenesis repair materials.
Manufacture method of the present invention can also adopt following technical scheme:
Described pore occupy-place particle is a sodium chloride crystal, and size is respectively 425-500 μ m, 600-750 μ m.
(weight ratio of β-TCP) is respectively: 1: 1,1: 2,1: 3 for described polyglycolic acid (PGA) and bata-tricalcium phosphate.
Described organic solvent is 1,1,1,3,3,3-hexafluoroisopropanol (HFIP).
Advantage and good effect that the present invention has are: through experiment in vitro and zoopery, the result shows that bone renovating bracket material of the present invention has: excellent biological compatibility, biodegradable and bone conductibility, can quicken the area of new bone ore deposit, and possess following characteristic:
A. material be easy to come with the particulate size of sodium chloride crystal the air vent aperture size of effective control material, and cost is low, wide material sources because the pore occupy-place particle that adopts is a sodium chloride crystal;
Obtain after the filtering of b sodium chloride crystal, coarse material surface is easy to osteoblast and attaches growth, because the pore occupy-place particle that adopts is a sodium chloride crystal, so the average pore size of pore is 440-810 μ m, the seriality of high porosity of timbering material (the average porosity is at 88.4%-93.6%) and pore is easy to cell fast to material internal propagation, diffusion;
C. and suitable material mixture ratio (weight of PGA and β-TCP), the vivo degradation speed that has more obtained material is suitable with bone formation speed, make material in whole osteogenetic process, can slowly discharge β-TCP particle in the body fluid environment around the material, thereby induce, accelerate bone formation rate continuously;
D. the anatomic form of repairing the position after bone is rebuild is good, but the degraded of the material all biological of defect filling, and substituted by new life's osseous tissue;
E. required equipment all is easy to obtain in the entire making process of the present invention, and low price.The material process is easy, easy operating.Compound support frame material of the present invention can bear autoclave sterilization, is easy to sterilization.Material after the sterilization only needs dry sealing simultaneously, can use at any time, is easy to storage and transport.
In a word, material of the present invention is because the pore occupy-place particle that adopts is a sodium chloride crystal, so it is low promptly relatively to be easy to making and cost, also be easy to preserve, and solved the problem that exists when bioactive ceramics and biodegradable polymer use separately respectively, it character that possesses explanation material of the present invention can be used as the osteanagenesis repair materials that osseous tissue is rebuild usefulness.
Description of drawings
Fig. 1 is the sketch map of material method of the present invention;
Fig. 2 is the image of the scanning electron microscope of six kinds of supports of the present invention;
Fig. 3 is the SEM image of six kinds of supports of the present invention;
Fig. 4 is the micro-CT image of the present invention's timbering material of containing agar;
Fig. 5 implants material diagram of the present invention in SD male rat artificial bone defective;
Fig. 6 is the postoperative micro-CT image of SD male rat;
Fig. 7, the 8th, the area of new bone bone amount increment figure of SD male rat operation back PGA/ β-bone defective region that the TCP support is repaired;
Fig. 9 is the different optical microscope image of putting the pathology section observing time;
Figure 10 is the biodegradation rate variation diagram of PGA/ β of the present invention-TCP support.
The specific embodiment
For further understanding content of the present invention, characteristics and effect, exemplify following examples now, and conjunction with figs. is described in detail as follows:
Mechanism of the present invention is: the macromolecular compound of polyglycolic acid and poly lactic acid series has good biocompatibility.Polyglycolic acid and β-calcium triphosphate can be finished biodegradation process through degraded and cytophagic dual function in vivo.The polyglycolic acid degradation in vivo becomes the glycolic monomer, its acid-base value is on every side reduced, and the catabolite of β-calcium triphosphate is an alkalescence, can carry out acid-base neutralization with glycolic, makes the suitable osteanagenesis of acid-base value of surrounding tissue.
Following formula is the chemical formula that two kinds of compositions are degraded in vivo and acid-base neutralization reacts.The catabolite of β-calcium triphosphate---calcium triphosphate is the necessary substrate of osseous tissue mineralising especially.But simple β-calcium triphosphate is difficult to make the timbering material that is fit to the damaged form of bone in the preparation, add plasticity macromolecular compound---polyglycolic acid good, that can carry out molten casting, form β-moulding support of calcium triphosphate particle with polyglycolic acid, just can address this problem.In the synthesized polymer material of biodegradable in vivo, have only polyglycolic acid to possess hydrophilic, uniformly dispersing, that have osteoinductive in addition β-calcium triphosphate particle, with the supporting structure that is communicated with pore, all suit body fluid and preosteoblast immerses the material internal of filling in the bone defective region fast, be beneficial to and osteoblasticly adhere to, break up, breed and spread, and help the transportation and the exchange of nutrient and metabolite, thereby more help the growth of cell, accelerated bone tissue regeneration speed and absorbed speed.
Below be the zooperal embodiment by the timbering material of two kinds of proportionings: the material that uses during preparation has: bata-tricalcium phosphate (β-TCP, three square crystals, mean diameter 100 μ m, weight average molecular weight 310.18units, density 3.14g/cm
3, purity is greater than 98%, and is Powdered); Polyglycolic acid (PGA, weight average molecular weight 100.000units, density 1.53g/cm
3, purity is greater than 99%, bulk); Organic solvent HFIP (1,1,1,3,3,3-hexafluoro-2-propanone); Sodium chloride crystal (NaCl), selecting particle size range with the standard screen mesh screen of 425 μ m and 500 μ m is 425-500 μ m.
As shown in Figure 1, the sketch map of material method.Polyglycolic acid PGA is 0.2g/ml (PGA/HFIP) with concentration at room temperature, is dissolved in fully among the organic solvent HFIP, forms polyglycolic acid PGA solution.Is mean diameter bata-tricalcium phosphate β-TCP particle adding polyglycolic acid PGA solution of 100nm, after concussion has been mixed into the homogeneous flowability, obtains containing the PGA/ β-TCP suspension of a large amount of microbubbles.Suspension is filled with in the glass model, is added rapidly a certain amount of sodium chloride crystal again, up to sodium chloride crystal no longer sedimentation and be full of model till.Through condense at normal temperatures (24 hours), solvent volatilization (48 hours), sodium chloride crystal filtering (72 hours, changed first water every 12 hours), sterilization, drying and other steps, finally make and have the new type compound osteanagenesis repair materials that is communicated with air hole structure.
[embodiment 1]
At room temperature, be that 0.2g/ml (PGA/HFIP) is dissolved among the organic solvent HFIP fully with concentration polyglycolic acid PGA, form polyglycolic acid PGA solution.Bata-tricalcium phosphate β-TCP powder is added polyglycolic acid PGA solution (weight ratio of PGA and β-TCP is 1: 1), after concussion has been mixed into the homogeneous flowability, obtain containing the PGA/ β-TCP suspension of a large amount of microbubbles.Suspension is filled with in the glass model, add rapidly more a certain amount of sodium chloride crystal (particle size range: 425-500 μ m), up to sodium chloride crystal no longer sedimentation and be full of model till.Through under room temperature (25 ℃), condense (24 hours), solvent volatilization (48 hours), sodium chloride crystal filtering (72 hours, changed first water every 12 hours), the high pressure high temperature vapor sterilization (is warming up to 126 ℃ gradually, behind the constant temperature 40 minutes, drop to room temperature more gradually), step such as normal temperature drying, finally obtain having PGA/ β-TCP (1: 1) the new type compound osteanagenesis renovating bracket material that is communicated with air hole structure.
By scanning electron microscope (SEM, Hitachi S-2700,20kV) image measurement, the air vent aperture that obtains PGA/ β-TCP (1: 1) support is 504.2 ± 93.5 μ m.
Recording its porosity by liquid displacement technique is 93.6 ± 2.0%.
Observe support-agar block by high-res tomoscan micro-CT, determine that it has the pore connectivity structure.
Animal bone defect repair experiment: at 10 ages in week, SD (Sprague Dawley, this bold and vigorous tired Ge Duolei) the distal femur inboard of male rat, damaged (the diameter 3mm of the bone that manually causes, dark 2mm) inserts in, PGA/ β-TCP support of 1: 1 is (according to the predetermined bone defective region size and the diameter 3mm of shape making, high 2mm), implant in the same bone defective region, as shown in Figure 5.In postoperative 14,30 and 90 days, use kinetic measurement that the toy viviperception carries out area of new bone bone amount and area of new bone bone density with the image of micro-CT relatively, and the observation analysis of histopathology section (H.E dyeing, promptly dye in " haematoxylin-Yihong ").
Show according to the 3 D stereo portrait behind toy viviperception usefulness high-res tomoscan micro-CT (80k V, the 120mA) image reduction, the bone form of the bone defective region that 1: 1 PGA/ β-TCP support is repaired, in operation back 90 days, promptly revert to the normal bone form.Cortical bone surface smoothing, eburnation, spongy bone are the honeycomb spline structure, and bone trabecula is clear, as Fig. 6: shown in the c-90.
Use 3D-
Bone analysis software (the Computerized three-dimensional image reconstruction of micro-CT scanning imagery, with sclerotin evaluation analysis system, atoc System Engineering, Tokyo, Japan) toy high-res tomoscan micro-CT image is carried out quantitative analysis, the result shows: the ratio (BV/TV) that the interior area of new bone bone amount of the bone defective region of 1: 1 PGA/ β-TCP support reparation accounts for the defective region tissue volume is 91.7 ± 5.0%, the area of new bone bone density is (in the equal volume, area of new bone content of mineral substances/organize content of mineral substances, BMD) 563.8 ± 26.9mg/cm
3
The optical microscope image of pathology section (H.E dyeing, * 40) shows: in the bone defective region that the PGA/ β that uses 1: 1-TCP support is repaired, the biodegradation rate of material and the reproduction speed of area of new bone are coordinated mutually.In postoperative 90 days, PGA/ β-TCP of 1: 1 was replaced by area of new bone substantially, and the newborn cortical bone and the form of spongy bone are similar to normal bone tissues, as Fig. 9: shown in the c-90.
As shown in Figure 10, use Canvas
TMX software (ACD Systems of America, Miami, Florida, USA) software carries out the biodegradation rate mensuration of PGA/ β-TCP support.90 days after surgery, PGA/ β-TCP degradation rate of 1: 1 was 98.97%.
[embodiment 2]
At room temperature, be that 0.2g/ml (PGA/HFIP) is dissolved among the organic solvent HFIP fully with concentration polyglycolic acid PGA, form polyglycolic acid PGA solution.Bata-tricalcium phosphate β-TCP powder is added polyglycolic acid PGA solution (weight ratio of PGA and β-TCP is 1: 3), after concussion has been mixed into the homogeneous flowability, obtain containing the PGA/ β-TCP suspension of a large amount of microbubbles.Suspension is filled with in the glass model, add rapidly more a certain amount of sodium chloride crystal (particle size range: 425-500 μ m), up to sodium chloride crystal no longer sedimentation and be full of model till.Through under room temperature (25 ℃), condense (24 hours), solvent volatilization (48 hours), sodium chloride crystal filtering (72 hours, changed first water every 12 hours), the high pressure high temperature vapor sterilization (is warming up to 126 ℃ gradually, behind the constant temperature 40 minutes, drop to room temperature more gradually), step such as normal temperature drying, finally obtain having PGA/ β-TCP (1: 3) the new type compound osteanagenesis repair materials that is communicated with air hole structure.
By scanning electron microscope (SEM, Hitachi S-2700,20kV) image measurement, the air vent aperture that obtains PGA/ β-TCP (1: 3) support is 483.3 ± 113.6 μ m, does not have significant difference with PGA/ β-TCP support of 1: 1.
Recording its porosity by liquid displacement technique is 88.4 ± 0.7%, is starkly lower than PGA/ β-TCP support (p＜0.001) of 1: 1.
Observe support-agar block by high-res tomoscan micro-CT, determine that it has the pore connectivity structure.
Animal bone defect repair experiment: at 10 ages in week, SD (Sprague Dawley, this bold and vigorous tired Ge Duolei) the distal femur inboard of male rat, damaged (the diameter 3mm of the bone that manually causes, dark 2mm) inserts in, PGA/ β-TCP support of 1: 3 is (according to the predetermined bone defective region size and the diameter 3mm of shape making, high 2mm), implant in the same bone defective region, as shown in Figure 5.In postoperative 14,30 and 90 days, the kinetic measurement that the image of using toy high-res tomoscan micro-CT carries out area of new bone bone amount and area of new bone bone density relatively, and the observation analysis of histopathology section (H.E dyeing).
Show according to the 3 D stereo portrait behind toy viviperception usefulness high-res tomoscan micro-CT (80k V, the 120mA) image reduction, the bone form of the bone defective region that 1: 3 PGA/ β-TCP support is repaired, in operation back 90 days, promptly revert to the normal bone form.Cortical bone surface smoothing, eburnation, the brightness of cancellous region is higher than embodiment 1, as Fig. 6: shown in the d-90, mean that bone density is higher than embodiment 1.
Use 3D-
The bone analysis software carries out quantitative analysis to the toy viviperception with high-res tomoscan micro-CT image, and the result shows: in the bone defective region that 1: 3 PGA/ β-TCP support is repaired, BV/TV is 96.4 ± 2.0%, and BMD is 606.3 ± 26.9mg/cm
3, bone density is higher than the area of new bone density (p＜0.05) of PGA/ β-TCP support of 1: 1 same period, has verified the iconography result of high-res tomoscan micro-CT once more.
The optical microscope image of pathology section (H.E dyeing, * 40) shows: in the bone defective region that the PGA/ β that uses 1: 3-TCP support is repaired, the biodegradation rate of material and the reproduction speed of area of new bone are coordinated mutually.In postoperative 90 days, PGA/ β-TCP of 1: 3 was replaced by area of new bone substantially, and the newborn cortical bone and the form of spongy bone are similar to normal bone tissues, as Fig. 9: shown in the d-90.
Use Canvas
TMX software (ACD Systems of America, Miami, Florida, USA) software carries out the biodegradation rate mensuration of PGA/ β-TCP support of 1: 3.90 days after surgery, PGA/ β-TCP degradation rate of 1: 3 was 96.18%, with PGA/ β-TCP support no significant difference of 1: 1 same period, as shown in figure 10.
As shown in Figure 7 and Figure 8, compare with the HAP matched group, postoperative 30 days and 90 days, the area of new bone bone amount more (p＜0.05) of the bone defective region that PGA/ β-TCP support is repaired, density higher (p＜0.05), i.e. osteanagenesis speed is faster.Particularly using weight ratio is the defective region of PGA/ β-TCP support reparation of 1: 3, area of new bone bone density the highest (p＜0.05).Pointed out the content of β-TCP in the mineralization degree of area of new bone and the material to be proportionate.
[embodiment 3]
At room temperature, be that 0.2g/ml (PGA/HFIP) is dissolved among the organic solvent HFIP fully with concentration polyglycolic acid PGA, form polyglycolic acid PGA solution.Bata-tricalcium phosphate β-TCP powder is added polyglycolic acid PGA solution, and the weight ratio of PGA and β-TCP is 1: 2, after concussion has been mixed into the homogeneous flowability, obtains containing the PGA/ β-TCP suspension of a large amount of microbubbles.Suspension is filled with in the glass model, add rapidly more a certain amount of sodium chloride crystal (particle size range: 425-500 μ m), up to sodium chloride crystal no longer sedimentation and be full of model till.Through under room temperature (25 ℃), condense (24 hours), solvent volatilization (48 hours), sodium chloride crystal filtering (72 hours, changed first water every 12 hours), the high pressure high temperature vapor sterilization (is warming up to 126 ℃ gradually, behind the constant temperature 40 minutes, drop to room temperature more gradually), step such as normal temperature drying, finally obtain having PGA/ β-TCP (1: 2) the new type compound osteanagenesis repair materials that is communicated with air hole structure, the aperture is 488 ± 30 μ m; The porosity is 93.6 ± 0.5 μ m.
[embodiment 4,5,6]
At room temperature, be that 0.2g/ml (PGA/HFIP) is dissolved among the organic solvent HFIP fully with concentration polyglycolic acid PGA, form polyglycolic acid PGA solution.Bata-tricalcium phosphate β-TCP powder is added polyglycolic acid PGA solution, embodiment 4,5, polyglycolic acid PGA in 6 and the weight ratio of bata-tricalcium phosphate β-TCP are followed successively by: 1: 1,1: 2,1: 3, after concussion has been mixed into the homogeneous flowability, obtain containing the PGA/ β-TCP suspension of a large amount of microbubbles.Suspension is filled with in the glass model, add rapidly more a certain amount of sodium chloride crystal (particle size range: 600-750 μ m), up to sodium chloride crystal no longer sedimentation and be full of model till.Through under room temperature (25 ℃), condense (24 hours), solvent volatilization (48 hours), sodium chloride crystal filtering (72 hours, changed first water every 12 hours), the high pressure high temperature vapor sterilization (is warming up to 126 ℃ gradually, behind the constant temperature 40 minutes, drop to room temperature more gradually), step such as normal temperature drying, finally obtained having PGA/ β-TCP larger aperture, that be communicated with air hole structure (1: 1,1: 2,1: 3) new type compound osteanagenesis repair materials, the aperture is followed successively by 738 ± 70 μ m, 724 ± 70 μ m, 710 ± 70 μ m; The porosity is followed successively by 93.9 ± 1.0 μ m, 93.8 ± 0.4 μ m, 89.3 ± 1.1 μ m.
[reference examples 1]
The damaged no reparation experiment No implant of animal bone: at 10 ages in week, SD (Sprague Dawley, this bold and vigorous tired Ge Duolei) the distal femur inboard of male rat, damaged (the diameter 3mm of the bone that manually causes, dark 2mm) in, do not insert any material, directly sew up.
[reference examples 2]
Animal bone defect repair experiment: in 10 ages in week, SD (Sprague Dawley, this sprinkles tired Ge Duolei) the distal femur inboard of male rat, the bone that manually causes damaged (diameter 3mm, dark 2mm), the hydroxyapatite of clinical practice (HAP, 100%,
, Japan), insert in the same bone defective region, as shown in Figure 5, sew up.
In postoperative 14,30 and 90 days, the kinetic measurement that the image of using toy high-res tomoscan micro-CT carries out area of new bone bone amount and area of new bone bone density relatively, and the observation analysis of histopathology section (H.E dyeing).
As shown in table 1, in six kinds of porous support materials, (weight ratio of β-TCP) is respectively: 1: 1,1: 2,1: 3, the size of sodium chloride crystal was respectively 425-500 μ m, 600-750 μ m for polyglycolic acid (PGA) and β-calcium triphosphate.
Table 1
Below be by external physical property observed result to the present invention---" PGA/ β-TCP porous support materials " description taken in conjunction with the accompanying drawings:
As shown in Figure 2, the image (amplification is 40 times) of the scanning electron microscope of these six kinds of supports (SEM, Hitachi S-2700,20k V).Pore in the timbering material is cubic, and the average pore size of pore also exists a large amount of, aperture in irregular shape on the frame wall of pore between 440～810 μ m, causes between the pore to connect, and forms to be communicated with pore.Helping blood flow and cell immerses, breeds to material internal fast.
As shown in Figure 3, the SEM image of six kinds of supports (amplification is 2000 times), the connection pore rack surface that visible bata-tricalcium phosphate particle and polyglycolic acid are formed also has microcellular structure, and the size of micropore is that 1～5 μ m does not wait.And the bata-tricalcium phosphate particle particle that as seen is coated in the polyglycolic acid PGA has formed uniform protrusion of surface, therefore shows coarse rack surface.The rough surface of this biologically active helps inducing cell and adheres to, and is divided into osteoblast, quickens bone formation rate.The roughness of rack surface becomes positive correlation with the weight ratio of the interior β of material-TCP particle.
As shown in Figure 4, be the high-res tomoscan micro-CT image of the present invention's timbering material of containing agar.Be that timbering material is soaked in 60 ℃ of transparent agar, till no longer ascension goes out bubble, with agar jointly through solidifying under the room temperature, cut out the agar block of coated stent material, carry out again that high-res tomoscan micro-CT photography obtains.Image shows that the pore in the support can be filled up fully by agar, and promptly agar can enter in all pores smoothly, and this has just confirmed the pore connectedness of this timbering material.
Claims (7)
1. the compound bone reparation timbering material of a perforated biodegradable, it includes is made by the complex of bioceramic particle and biodegradation high molecular, have air hole structure, the biodegradable osteanagenesis repair materials of connection, it is characterized in that: the average pore size of described connection pore is 440-810 μ m.
2. the compound bone reparation timbering material of perforated biodegradable according to claim 1, it is characterized in that: described bioactive ceramics particle, being bioactive ceramics with osteoinductive---(β-TCP), density is 3.14g/cm to bata-tricalcium phosphate
3, be Powdered.
3. the compound bone reparation timbering material of perforated biodegradable according to claim 1, it is characterized in that: described biodegradation high molecular is the biodegradation high molecular of possess hydrophilic property---polyglycolic acid (PGA), density are 1.53g/cm
3
4. the manufacture method of the compound bone renovating material of a perforated biodegradable, it is characterized in that: (PGA) fully is dissolved in the organic solvent the biodegradation high molecular polyglycolic acid, add powdered biological active ceramic particle bata-tricalcium phosphate (β-TCP) again, fully behind the concussion mixing, with the prefabricated model of the common injection of pore occupy-place particle, through condensing, the solvent volatilization, the filtering of pore occupy-place particle, sterilization, dry, final obtain that (β-TCP) and biodegradation high molecular polyglycolic acid (PGA) complex make, and has to be communicated with air hole structure with bioactive ceramics particle bata-tricalcium phosphate, porous, biodegradable osteanagenesis repair materials.
5. the manufacture method of the compound bone renovating material of perforated biodegradable according to claim 4, it is characterized in that: described pore occupy-place particle is a sodium chloride crystal, size is respectively 425-500 μ m, 600-750 μ m.
6. the manufacture method of the compound bone renovating material of perforated biodegradable according to claim 4 is characterized in that: (weight ratio of β-TCP) is respectively: 1: 1,1: 2,1: 3 for described polyglycolic acid (PGA) and bata-tricalcium phosphate.
7. the manufacture method of the compound bone renovating material of perforated biodegradable according to claim 4, it is characterized in that: described organic solvent is 1,1,1,3,3,3-hexafluoroisopropanol (HFIP).
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| CN103263692A (en) * | 2013-06-06 | 2013-08-28 | 四川大学 | Preparation method of bone repair bracket with osteoid poriform regeneration channel structure |
| CN107998452A (en) * | 2017-11-29 | 2018-05-08 | 成都创客之家科技有限公司 | A kind of Cleft palate repai prosthese |
| CN114848921A (en) * | 2022-05-17 | 2022-08-05 | 山东建筑大学 | Preparation method of reproducible functional gradient bone scaffold by 3D printing |
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| CN114848921A (en) * | 2022-05-17 | 2022-08-05 | 山东建筑大学 | Preparation method of reproducible functional gradient bone scaffold by 3D printing |
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