CN101779578B - Method for using fungus to elicit dragon tree to produce sangusis draconis - Google Patents
Method for using fungus to elicit dragon tree to produce sangusis draconis Download PDFInfo
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- CN101779578B CN101779578B CN2009100002576A CN200910000257A CN101779578B CN 101779578 B CN101779578 B CN 101779578B CN 2009100002576 A CN2009100002576 A CN 2009100002576A CN 200910000257 A CN200910000257 A CN 200910000257A CN 101779578 B CN101779578 B CN 101779578B
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Abstract
The invention relates to a method for using fungus to elicit a dragon tree to produce sangusis draconis, belonging to the biotechnological field. One used fungi is a Fusaria (Fusarium sp.) fungus D-22. The content comprises the culture method and the inoculation method of the fungi. By adopting the method, the trunk of the dragon tree to elicited to secrete red substances similar to the chemical ingredients of sangusis draconis. The invention has the advantages that the method is simple and convenient, the production cost is low, the period is short, the dragon tree resources are fully utilized and protected, and the popularization and the application are facilitated.
Description
Technical field
The invention belongs to technical field of bioengineering, relate in particular to a kind of method of utilizing fungus to elicit dragon tree to produce sangusis draconis.
Background technology
Dragon's blood (dragon ' s blood) be a kind of peony resin, be used for many countries as famous traditional medicinal material from ancient times.Be mainly used in clinically and stimulate circulation treatment wound, inflammation, dysentery and diabetes.According to the literature, dragon's blood in the market is mainly derived from 4 sections, 5 genus, 23 plant species.In China, the seventies in last century, Mr. Cai Xitao has found that in Yunnan Province the alternative import dragon's blood of the red resin of swordleaf dragon tree [Dracaena cochinchinensis (Lour.) S.C.Chen] uses, and has thoroughly changed the history of the long-term import of dragon's blood.Follow-uply found in Hainan Province that again homemade dragon's blood exhausts another basic source plant Folium Dracaenae cambodianae (D.cambodiana Pierre ex Gagnep.) of (Chinesedragon ' s blood).
In recent years, the demand sharp increase to domestic Dragon Blood makes the reserves of swordleaf dragon tree and Folium Dracaenae cambodianae wild resource sharply descend, and has been listed in the rare protective plant in imminent danger of country.Make the development and use of dragon's blood be restricted, therefore extensively ward off the production approach, solve dragon's blood natural resources shortage problem and become very urgent.
The formation that studies show that dragon's blood at present has confidential relation with the damage and the infecting of fusarium fungus of setting body.This shows that fungi has very important effect in the production process of dragon's blood.Under natural environmental condition, the output of dragon's blood is not only low, and the production cycle is long.Therefore, artificial inoculation specified microorganisms is expected to improve the output of dragon's blood, and shortens the time that produces dragon's blood significantly, perhaps is that one of approach of application prospect is arranged most.
Summary of the invention
The object of the present invention is to provide 1 strain can induce dragon tree to produce the fungi of dragon's blood, this fungi be from the stem portion of dragon tree from obtaining, it can promote live body dragon tree trunk and stripped dragon tree trunk to produce the red material similar to the dragon's blood chemical composition.
Another purpose of the present invention is to provide a kind of method of using fungus to elicit dragon tree to produce sangusis draconis, and this method technology is simple, low production cost; with short production cycle; utilize fully and protected the dragon tree resource, easy to utilize, solve the problem of dragon's blood medicine resource anxiety.
For achieving the above object, the technology that the present invention adopts is to cultivate fungi earlier, fungi is inoculated on the trunk of dragon tree again.After after a while, around the inoculation hole, can produce the red material similar to the dragon's blood chemical composition.1 fungal strain that the present invention adopts is through being accredited as Fusarium fungi D-22 (Fusarium sp.), deposit number CGMCC No.2598, this bacterial strain on July 16th, 2008 in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, depositary institution address: No. 3, A, DaTun Road, Chaoyang District, BeiJing City, Institute of Microorganism, Academia Sinica.The preservation of bacterial strain and survival proof are seen Appendix.
The cultural character of fungi D-22 is: bacterium colony is a pink colour on the PDA medium, and aerial hyphae is thread, and the bacterium colony back side is bronzing, secretion bronzing material, and the 1 all colony diameters of growing are 5.2cm; The microconidia unit cell, ellipse, two ends are slightly crooked sometimes, 3.4-12.1 * 1.4-3.4 μ m; Macroconidium, many born of the same parents, sickleshaped, 37.6 * 4.0 μ m, rare.
Specifically, technical step method of the present invention is as follows:
1. the cultivation of fungi
Will by the dragon tree stem portion from the deposit number that obtains be the fungi D-22 of CGMCC No.2598 behind the slant tube actication of culture of low-temperature preservation, transfer in the plate of PDA medium constant temperature culture 15-20 days; Or transfer in PDA liquid nutrient medium or wheat bran liquid nutrient medium, isothermal vibration was cultivated 5-15 days, or transferred in sawdust wheat bran solid culture medium constant temperature culture 20-50 days; Cultivation temperature is 22-28 ℃.
The bacterial classification inoculation of above-mentioned cultivation is cultivated in the sawdust complex solid medium that contains sawdust, leaf and wheat bran, treated directly to use after mycelia is covered with medium.The weight ratio of sawdust, leaf and wheat bran is 2: 1: 0.5 in the sawdust complex solid medium; Or, punch into the bacterium sheet at colony edge with the bacterial classification that the PDA plate is cultivated, and insert in PDA liquid nutrient medium or the wheat bran liquid nutrient medium, cultivate and get the fermentation product use after 5-15 days.
2. the inoculation of fungi
Choose the swordleaf dragon tree of the age of tree more than 5 years or the trunk or the stripped trunk of Folium Dracaenae cambodianae, mode by boring forms diameter 0.5-3cm at trunk, the inoculation hole of dark 2-15cm, or by transverse cuts or vertically cutting or the mode of oblique cutting from the top down, form long 1-6cm at trunk, wide 1-6cm, the inoculating groove of dark 1-6cm.That inoculate hole or inoculating groove is longitudinal separation 5-15cm at interval, lateral separation 3-10cm.In the inoculation hole, insert the bacterial classification of sawdust complex solid medium culture or the fermentation product that liquid nutrient medium is cultivated, in inoculating groove, insert the bacterial classification of sawdust complex solid medium culture.Inoculating hole is wrapped up with polypropylene film in the inoculation back.After 3-10 month, inoculation can produce the red material similar to the dragon's blood chemical composition around the hole.
Embodiment
Embodiment 1
The fungi D-22 that cultivates in the PDA medium plate is seeded on the sawdust complex solid medium and grew 30 days, and is standby.
Choose the age of tree and be the Folium Dracaenae cambodianae in 10 years, with diameter is that the drill bit of 1cm is holed on trunk 9, hole depth is 5-6cm, interval between each hole is 10cm, wherein insert in 3 holes after the sterilization sawdust complex solid medium in contrast, insert 30 days fungi D-22 of above-mentioned cultivation in other 6 holes, polypropylene film wrapping inoculating hole.
Observe after 6 months, all redfree material secretion generations around the hole of contrast all have the red material secretion to produce around the hole of access fungi D-22.Present red xylem around collecting hole, extract the back and detect, confirm that its chemical composition to dragon's blood is similar.Observe the back and insert fungi D-22 once more at former inoculation position.
Embodiment 2
The fungi D-22 that cultivates in the PDA medium plate was seeded in the PDA liquid nutrient medium fermented and cultured 10 days, collected fermentation product, and the sterilization back is standby.
Choose the age of tree and be the Folium Dracaenae cambodianae in 8 years, with diameter is that the drill bit of 0.2cm is holed on trunk 9, hole depth is 7-8cm, interval between each hole is 10cm, wherein insert in 3 holes after the sterilization the PDA liquid nutrient medium in contrast, fungi D-22 fermentation product in other 6 holes after the above-mentioned sterilization of access, polypropylene film wrapping inoculating hole.
Observe after 6 months: all redfree material secretion generations around the hole of contrast all have the red material secretion to produce around the hole of access fungi D-22 fermentation product.Present red xylem around collecting hole, extract the back and detect, confirm that its chemical composition to dragon's blood is similar.
Embodiment 3
The fungi D-22 that the wheat bran liquid nutrient medium is cultivated is seeded on the sawdust complex solid medium and grew 30 days, and is standby.
Choosing diameter is the swordleaf dragon tree juggle of 10-25cm, and juggle is carried out inactivation treatment.With diameter is that the drill bit of 1cm is holed on juggle 9, hole depth is 5-6cm, interval between each hole is 10cm, wherein insert in 3 holes after the sterilization sawdust complex solid medium in contrast, insert 30 days fungi D-22 of above-mentioned cultivation in other 6 holes, polypropylene film wrapping inoculating hole.
Juggle is placed in 24-26 ℃ of environment and is observed after 6 months, and all redfree material secretion generations around the hole of contrast all have the red material secretion to produce around the hole of access fungi D-22 fermentation product.Present red xylem around collecting hole, extract the back and detect, confirm that its chemical composition to dragon's blood is similar.
Embodiment 4
The fungi D-22 that cultivates in the PDA medium plate was seeded in the PDA liquid nutrient medium fermented and cultured 10 days, collected fermentation product, and the sterilization back is standby.
Choosing diameter is the swordleaf dragon tree juggle of 10-25cm, and juggle is carried out inactivation treatment.With diameter is that the drill bit of 0.2cm is holed on juggle 9, hole depth is 7-8cm, interval between each hole is 10cm, wherein insert in 3 holes after the sterilization the PDA liquid nutrient medium in contrast, fungi D-22 fermentation product in other 6 holes after the above-mentioned sterilization of access, polypropylene film wrapping inoculating hole.
Juggle is placed in 24-26 ℃ of environment and is observed after 6 months, and all redfree material secretion generations around the hole of contrast all have the red material secretion to produce around the hole of access fungi D-22 zymotic fluid.Present red xylem around collecting hole, extract the back and detect, confirm that its chemical composition to dragon's blood is similar.
Embodiment 5
The fungi D-22 that sawdust wheat bran solid culture medium is cultivated is seeded on the sawdust complex solid medium and grew 30 days, and is standby.
Choose the age of tree and be the swordleaf dragon tree in 8 years, on trunk, adopt following 4 kinds of modes to form the inoculation hole or connect the bacterium groove: (1) boring, inoculation bore dia 1cm, dark 6cm; (2) transverse cuts forms long 5cm, wide 2cm, the groove of dark 3cm; (3) vertically cutting forms long 2cm, wide 5cm, the groove of dark 3cm; (4) oblique from the top down interior cutting forms long 5cm, wide 0.5cm, the groove of dark 3cm.The interval of each inoculation hole or inoculating groove is 10cm.Insert 30 days fungi D-22 of above-mentioned cultivation in the inoculation hole of mode (1)-(4) or inoculating groove, the polypropylene film wrapping connects the bacterium position.More than every kind of mode repeat 3 times.
Observe after 6 months: the inoculation hole or the inoculating groove that insert fungi D-22 all have the red material secretion to produce on every side.Present red xylem around collecting hole, extract the back and detect, confirm that its chemical composition to dragon's blood is similar.
Claims (5)
1. the method for a using fungus to elicit dragon tree to produce sangusis draconis comprises step:
(1) cultivation of fungi
Will by dragon tree separate the deposit number that obtains be the fungi D-22 of CGMCC No.2598 behind the slant tube actication of culture of low-temperature preservation, transfer in the plate of PDA medium constant temperature culture 15-20 days; Or transfer in the PDA liquid nutrient medium, isothermal vibration was cultivated 5-15 days, or transferred in sawdust wheat bran solid culture medium constant temperature culture 20-50 days;
The bacterial classification inoculation of above-mentioned cultivation is cultivated in sawdust complex solid medium, treated directly to use after mycelia is covered with medium; Sawdust complex solid medium contains sawdust, leaf and wheat bran, and their weight ratio is 2: 1: 0.5; Or the bacterial classification of PDA plate cultivation, punch into the bacterium sheet at colony edge, insert in the PDA liquid nutrient medium, cultivate and get the fermentation product use after 5-15 days;
(2) cultural character of fungi
Deposit number is that the bacterial strain D-22 of CGMCC No.2598 is Fusarium (Fusarium sp.) fungi, and bacterium colony is a pink colour on the PDA medium, and aerial hyphae is thread, and the bacterium colony back side is bronzing, secretion bronzing material, and the 1 all colony diameters of growing are 5.2cm; The microconidia unit cell, ellipse, two ends are slightly crooked sometimes, 3.4-12.1 * 1.4-3.4 μ m; Macroconidium, many born of the same parents, sickleshaped, 37.6 * 4.0 μ m, rare;
(3) inoculation of fungi
The method of inoculated fungi is: choose the dragon tree trunk of the age of tree more than 5 years, form diameter 0.5-3cm by the boring or the mode of grooving at trunk, inoculation hole or the long 1-6cm of dark 2-15cm, wide 1-6cm, the inoculating groove of 1-6cm deeply; In inoculation hole or inoculating groove, insert the bacterial classification of sawdust complex solid medium culture, or insert the fermentation product that liquid nutrient medium is cultivated; Inoculating hole or inoculating groove are wrapped up with polypropylene film in the inoculation back;
Inoculated fungi is once on the trunk of dragon tree with said method, or behind first time inoculated fungi, around the inoculation hole, form the initial stage or the mid-term of dragon's blood sample red material, form in the zone, with said method repeated inoculation fungi at former inoculation position or at red material.
2. method according to claim 1 is characterized in that: the seeds that this method is suitable for are Liliaceae dracaena Folium Dracaenae cambodianae and swordleaf dragon tree.
3. method according to claim 1 is characterized in that: be used for punching or grooving, the dragon tree trunk of inoculated fungi D-22 is live body dragon tree trunk or the age of tree the stripped dragon tree trunk 5 year or more of the age of tree more than 5 years.
4. method according to claim 1 is characterized in that: the mode of digging inoculating groove at trunk comprises that transverse cuts forms groove, and vertically cutting forms groove, or oblique from the top down formation groove.
5. method according to claim 1 is characterized in that: that inoculation hole or inoculating groove is longitudinal separation 5-15cm at interval on the dragon tree trunk, lateral separation 3-10cm.
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| CN102649938B (en) * | 2011-02-24 | 2013-05-29 | 中国医学科学院药用植物研究所 | Two fungi for inducing dragon tree to produce dragon's blood |
| CN110699264B (en) * | 2019-11-08 | 2021-03-05 | 山东省花生研究所 | Rapid culture method of pathogenic fungi of peanut rot |
| CN116267294B (en) * | 2022-12-06 | 2024-05-07 | 中国医学科学院药用植物研究所海南分所 | Method for inducing dragon trees to continuously produce dragon's blood |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1821387A (en) * | 2006-03-09 | 2006-08-23 | 中国科学院西双版纳热带植物园 | Longxuejie inducing agent and its preparing method |
| CN101032207A (en) * | 2007-04-12 | 2007-09-12 | 中国热带农业科学院热带生物技术研究所 | Method of inducing dragon trees planted by human beings to generate dragon's blood |
| CN101138305A (en) * | 2007-09-14 | 2008-03-12 | 云南大唐汉方制药有限公司 | Method of fast obtaining rough material of dragon's blood |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1821387A (en) * | 2006-03-09 | 2006-08-23 | 中国科学院西双版纳热带植物园 | Longxuejie inducing agent and its preparing method |
| CN101032207A (en) * | 2007-04-12 | 2007-09-12 | 中国热带农业科学院热带生物技术研究所 | Method of inducing dragon trees planted by human beings to generate dragon's blood |
| CN101138305A (en) * | 2007-09-14 | 2008-03-12 | 云南大唐汉方制药有限公司 | Method of fast obtaining rough material of dragon's blood |
Non-Patent Citations (3)
| Title |
|---|
| 罗文扬等.珍稀濒危龙血竭基源植物——龙血树.《中国种业》.2008,(第3期),57-59. * |
| 罗文扬等.珍稀濒危龙血竭基源植物龙血树的资源现状.《现代农业科技》.2007,(第22期),62-64. * |
| 蔡文伟等.龙血树血竭生物合成相关基因分离与分析.《分子植物育种》.2008,第6卷(第5期),881-885. * |
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