CN101520418B - Detection device and method - Google Patents

Abstract

A detection device is used to obtain the concentration of a sample, and includes a first detection unit, a signal control unit and a shell. The first detection unit detects the intensity of the light to be measured. The signal control unit calculates the intensity of the light to be measured and a correction value, and obtains the concentration of the sample according to the calculation result. The shell covers the first detection unit and the signal control unit. The correction value is related to the environment around the shell. The present invention also provides a detection method for obtaining the concentration of the sample in the shell.

Description

Detection device and method

technical field

The invention relates to a detection device and method, in particular to a detection device and method for measuring sample concentration.

Background technique

The existing method of measuring the concentration of the sensing substance is to use a crystal oscillator. When detecting the concentration, an adsorption layer for adsorbing the sensing substance is formed on the surface of the crystal oscillator. The sensing substance is attached to the adsorption layer, and the frequency of the crystal sensor is changed with the adhesion amount of the adsorption layer to obtain the concentration of the sensing substance.

With the advancement of technology, more detection methods have been developed. Among the existing medical detection methods, a spectrum analyzer is a commonly used instrument, which uses the principle that when light penetrates a certain substance, the light of sub-wavelengths will be absorbed by the substance to perform quantitative analysis on the substance. Spectrum analyzers know the concentration of a substance based on the light that passes through the substance. However, at the same time of detection, if there is additional light irradiating the substance, it will affect the analysis results of the spectrometer.

Contents of the invention

The invention provides a detection device for obtaining the concentration of a sample (specimen), and includes a first detection unit, a signal regulation unit and a casing. The first detection unit detects the intensity of the light to be detected. The signal regulation unit calculates the intensity of the light to be measured and the correction value, and obtains the concentration of the sample according to the calculation result. The casing covers the first detection unit and the signal regulation unit. The correction value is related to the environment around the shell.

The housing in the detection device has a selection switch, and the user switches the selection switch according to the environment of the detection device.

The signal regulation unit in the detection device determines the level of the correction value according to the state of the selection switch.

The correction value in the detection device is related to the light around the housing.

The light to be measured in the detection device is emitted by the sample.

In the detection device, chemical substances are added to the sample to make the sample emit the light to be measured.

The detection device also includes a light-emitting element, which is arranged in the casing, and when the light-emitting element illuminates the sample, the light that can penetrate the sample is the light to be detected.

The first detection unit in the detection device also detects the light intensity around the housing to generate the correction value.

The first detection unit in the detection device first detects the intensity of light around the housing, and then detects the intensity of the light to be detected.

The casing in the detection device has a first light-transmitting hole for receiving light around the casing.

The first detection device in the detection device is arranged at a mapping position of the first light transmission hole.

In the detection device, when the first detection device detects the light to be measured, the sample is arranged between the first light transmission hole and the first detection device.

The housing in the detection device also has a second light hole for receiving light around the housing.

The detection device further includes a second detection unit, the second detection unit is arranged at the mapping position of the second light transmission hole, and is used to detect the light intensity around the housing to generate the correction value.

In the detection device, when the first detection unit detects the intensity of the light to be measured, the second detection unit also detects the intensity of light around the casing synchronously.

The first detection unit in the detection device is a charge-coupled device (Charge-coupled Device; CCD) or a complementary metal-oxide semiconductor (Complementary Metal-Oxide Semiconductor; CMOS).

The invention also provides a detection method for obtaining the concentration of the sample in the housing. The detection method includes the following steps: obtaining a correction value, which is related to the surrounding environment of the housing; obtaining the intensity of the light to be measured according to the sample; calculating the intensity of the light to be measured and the correction value; and according to the calculation result , to obtain the concentration of the sample.

The correction value in this detection method is also related to the light around the housing.

The correction value in the detection method is obtained simultaneously or sequentially with the light to be measured.

The light to be measured in the detection method is emitted by the sample.

In order to make the above and other objects, features and advantages of the present invention more comprehensible, preferred embodiments are listed below and described in detail in conjunction with the accompanying drawings.

Description of drawings

Fig. 1 is a possible embodiment of the detection device of the present invention.

Figure 2 shows a possible embodiment of the housing.

Fig. 3 is another possible embodiment of the detection device of the present invention.

4a-4e are schematic appearance diagrams of the casing.

Figures 5a-5d show another possible embodiment of the housing.

Fig. 6 is another possible embodiment of the detection device of the present invention.

Figures 7a-7c show another possible embodiment of the housing.

Fig. 8 is another possible embodiment of the housing.

Fig. 9 is a flow chart of the detection method of the present invention.

Detailed ways

Fig. 1 is a possible embodiment of the detection device of the present invention. The detecting device 110 is used for detecting the concentration of the sample 120 . As shown in the figure, the detection device 110 includes a detection unit 111 , a signal regulation unit 112 , a housing 113 , a selection switch 114 and a light emitting element 115 . The sample 120 may be placed using a sample box (not shown). When the sample cartridge with the sample 120 is inserted into the housing 113 , the detection device 110 can detect the sample 120 to obtain the concentration of the sample 120 .

The detection unit 111 detects the intensity of the light L ₁ to be measured. The detection unit 111 can be a Charge-coupled Device (CCD) or a Complementary Metal-Oxide Semiconductor (CMOS). The light to be measured L ₁ detected by the detection unit 111 comes from the sample 120 . In this embodiment, the light to be measured L ₁ is the light that passes through the sample 120 . When the light emitting element 115 irradiates the sample 120 , the light passing through the sample 120 is the light L ₁ to be measured.

In other embodiments, the light L ₁ to be measured is the light emitted by the sample 120 . When the sample is mixed with chemical substances, it can emit light L ₁ to be measured. For example, if a compound is added to sample 120, hydrogen peroxide can be produced. Through the chemical changes of hydrogen peroxide, developing agent and peroxidase, luminescence, fluorescence, visible light or ultraviolet light can be produced. Therefore, the sample 120 can emit light. The developer can be luminol, or 2-methyl indole, etc. The compound that generates hydrogen peroxide with the sample may be uricase, a mixture of phospholipase and cholinease, or a mixture of lipolytic enzyme and glycerolase.

The signal regulation unit 112 calculates the intensity of the light _L1 to be measured and a correction value, and obtains the concentration of the sample 120 according to the calculation result, wherein the correction value is related to the environment (such as light) around the housing 113 . Due to the influence of external light, when the detection device 110 detects the concentration of the sample 120 under different light environments, the detection results obtained may be affected by the aforementioned external environment. However, the signal regulating unit 112 calculates the intensity of the light L ₁ to be measured and the correction value, and the correction value is related to the environment around the casing 113 , so the influence caused by the environment around the casing 113 can be eliminated.

In a possible embodiment, the signal regulation unit 112 may have a built-in environmental impact database. When the environment around the casing 113 changes, the signal regulation unit 112 can obtain a corresponding correction value from the environmental impact database to correct the result detected by the detection unit 111 .

For example, if the detection device 110 is placed outdoors, the user can switch the selector switch 114 to the outdoor state. Therefore, the signal conditioning unit 112 can obtain the relevant information from the environmental impact database according to the state of the selector switch 114. corresponding correction value. If the detection device 110 is placed indoors, the user can switch the selection switch 114 to the indoor state, so the signal control unit 112 can obtain the correction value corresponding to the indoor state from the environmental impact database.

The casing 113 is used to cover the detection unit 111 , the signal regulation unit 112 and the light emitting element 115 , wherein the selection switch 114 is disposed on the surface of the casing 113 for the convenience of the user to switch. Possible forms of the housing 113 will be described below. FIG. 2 shows a possible embodiment of the housing. As shown in the figure, the housing 113 has a slot 210 and selection switches SW ₁ -SW _n .

The slot 210 is used for placing the sample box. When the sample box with the sample is placed in the slot 210, the concentration of the sample can be measured in conjunction with the switching states of the selector switches SW ₁ -SW _n , wherein the detection result has been ruled out caused by the surrounding environment of the housing 113. Impact.

In this embodiment, the selection switch SW ₁ has an indoor state and an outdoor state; the selection switch SW ₂ has a sunny state, a rainy state, and a cloudy state; and the selection switch SW _n has a morning state, a noon state, and an evening state. By switching the states of the selection switches SW ₁ -SW _n , even if the detection device is in different environments, the same detection result can still be obtained for the same sample.

Fig. 3 is another possible embodiment of the detection device of the present invention. As shown in the figure, the detection device 310 has detection units 311 , 312 , a signal regulation unit 313 and a housing 314 . The detection unit 311 is used for detecting the light L ₂ from the sample 320 . Since the housing 314 has a light-transmitting hole 421 , the light L ₂ is affected by the light _LE around the housing 314 . The detection unit 312 detects the intensity of the light _LE around the casing 314 through the light transmission hole 422 . The signal regulation unit 313 calculates the detection results of the detection units 311 and 312 to compensate the influence caused by the light _LE . In this embodiment, the signal regulation unit 313 uses the detection result of the detection unit 312 as a correction value to correct the detection result of the detection unit 311 .

The casing 314 covers the detection units 311 , 312 and the signal regulation unit 313 . One possible form of housing will be described below. Fig. 4a is a schematic diagram of the appearance of the housing. As shown in the figure, the housing 314 has a slot 410 and light holes 421 and 422 . Figure 4b is a top view of the housing (sample cartridge not inserted). As shown in the figure, the dashed box 431 is the placement position of the sample box. Figure 4c is a top view of the housing (sample cartridge inserted). Figure 4d is a side view of the housing (sample cartridge not inserted). As shown in the figure, the detection units 311 and 312 are respectively placed at the mapping positions of the light holes 421 and 422 (positions that can accept light passing through the light holes for light sensing). Figure 4e is a side view of the housing (sample cartridge inserted). As shown in the figure, when the sample box 441 is inserted into the slot 410 , it is located between the light transmission hole 421 and the detection unit 311 .

Another possible embodiment of the housing will be described below. Figure 5a is a top view of the housing (sample cartridge not inserted). As shown in the figure, the dotted box 510 is where the sample box is placed. Figure 5b is a top view of the housing (sample cartridge inserted). Reference numeral 531 is a sample. Figure 5b differs from Figure 4c in the size of the sample box. Although the sample box in Figure 5b is larger than the sample box shown in Figure 4c, the sample 531 is only placed on one side of the sample box in Figure 5b. Figure 5c is a side view of the housing (sample cartridge not inserted). Figure 5d is a side view of the housing (sample cartridge inserted). As shown in the figure, although the sample box 541 can span over the detection unit 312 , it only has the sample 531 above the detection unit 311 .

Fig. 6 is another possible embodiment of the detection device of the present invention. FIG. 6 is similar to FIG. 4 , except that the detection device 610 in FIG. 6 only has a detection unit, and the housing 613 has only a single light-transmitting hole. Please refer to FIG. 6 , when the sample box with the sample 620 is not placed in the detection device 610 , the detection unit 611 first detects the intensity of the light _LE around the housing 613 , and then transmits the detection result to the signal control unit 612 . When the sample box is placed at the detection device 610 , the detection unit then detects the intensity of the light L ₃ to be measured, and transmits the detection result to the signal control unit 612 . The signal conditioning unit 612 calculates the detection results twice to obtain the luminous intensity of the sample 620 . In another possible embodiment, the intensity of the light L ₃ may also be detected first, and then the intensity of the light _LE around the casing 613 is detected.

Figure 7a is another possible embodiment of the housing. In this embodiment, the housing 613 has a slot 721 and a light-transmitting hole 722 . Figure 7b is a top view of the housing (sample cartridge not inserted).

During the period from time t0 to ts, since the sample cartridge is not inserted, the intensity of light _LE is detected first. Figure 7c is a top view of the housing (sample cartridge inserted). During the period from ts to te, since the sample cartridge is inserted, the intensity of _L3 can be detected. The signal regulation unit 612 uses the result detected by the detection unit 611 during the period t0˜ts as a correction value to correct the result detected by the detection unit 611 during the period ts˜te.

Fig. 8 is another possible embodiment of the housing. In this embodiment, in addition to the detection unit (not shown) at the mapping position of the light transmission hole 722 , there is another detection element 731 on the surface of the housing 810 for detecting the intensity of light around the housing 810 .

Fig. 9 is a flow chart of the detection method of the present invention. The detection method of the present invention can obtain the concentration of the sample in the casing. Firstly, a correction value is obtained (step S910), wherein the correction value is related to the environment around the casing. In a possible embodiment, the correction value may be a detection value. When the environment around the shell (such as light) changes, the correction value will also change accordingly. In other embodiments, multiple correction values may be stored in advance. The user can switch the state of the selection switch according to the surrounding environment of the casing, so as to obtain an appropriate correction value from a plurality of correction values.

Then, according to the sample, the intensity of the light to be measured is obtained (step S920). The light to be measured comes from the sample. In one possible embodiment, the sample may be mixed with chemicals such that the sample emits light. In another possible embodiment, a light-emitting element (such as a light-emitting diode) can be used to illuminate the sample, wherein the light that can pass through the sample is the light to be measured. In other possible embodiments, the intensity of the light to be measured can be obtained first, and then the correction value can be obtained, or the correction value and the intensity of the light to be measured can be obtained at the same time.

Next, calculate the intensity of the light to be measured and a correction value (step S930). Since the correction value is related to the environmental factors around the housing, the correction value can be used to correct the light to be measured. Finally, according to the calculation result, the concentration of the sample can be obtained (step S940). Since the correction value is related to the surrounding environment of the casing, when calculating the intensity of the light to be measured and the correction value, the influence caused by the environmental factors around the casing can be excluded. Therefore, the concentration of the sample can be accurately obtained according to the calculation result.

In summary, when measuring the concentration of the sample, the surrounding environment (such as light) of the detection device may affect the measurement result, therefore, the detection device and method of the present invention can remove the influence of the measurement environment, and then obtain more accurate measurement results.

Although the present invention has disclosed the above preferred embodiments, it is not intended to limit the present invention. Any person of ordinary skill in the art should be able to make some changes and modifications without departing from the spirit and scope of the present invention. Therefore, the present invention The scope of protection shall prevail as defined by the appended claims.

Explanation of main component symbols

110, 310, 610: detection device;

111, 311, 312, 611, 731: detection unit;

112, 313, 612: signal control unit;

113, 314, 613, 810: shell;

114. SW ₁ ~ SW _n : selection switch;

115: light emitting element;

120, 320, 531, 620: samples;

210, 410, 721: slots;

421, 422, 521, 522, 722: light holes;

431, 511: dotted frame;

441, 541: sample box;

S910-S940: steps.

Claims (18)

1. a detection device, in order to obtain the concentration of sample, said detection device comprises: The first detection unit detects the intensity of the light to be measured; a signal control unit, calculating the intensity of the light to be measured and a correction value, and obtaining the concentration of the sample according to the calculation result; and The casing is used to cover the first detection unit and the signal regulation unit, wherein the correction value is related to light around the casing. 2. The detection device according to claim 1, wherein the housing has a selection switch, and the user switches the selection switch according to the environment of the detection device. 3. The detection device according to claim 2, wherein the signal regulation unit determines the level of the correction value according to the state of the selection switch. 4. The detection device according to claim 1, wherein the light to be measured is emitted by the sample. 5. The detection device according to claim 4, wherein chemical substances are added into the sample to cause the sample to emit the light to be detected. 6. The detection device according to claim 1, further comprising a light-emitting element arranged in the housing, when the light-emitting element irradiates the sample, the light that can penetrate the sample is the Light to be measured. 7. The detection device according to claim 1, wherein the first detection unit also detects light intensity around the housing to generate the correction value. 8. The detection device according to claim 7, wherein the first detection unit first detects the intensity of the light around the casing, and then detects the intensity of the light to be measured. 9. The detection device according to claim 8, wherein the casing has a first light-transmitting hole for receiving light around the casing. 10. The detection device according to claim 9, wherein the first detection unit is arranged at a mapping position of the first light transmission hole. 11. The detection device according to claim 10, wherein when the first detection unit detects the light to be measured, the sample is arranged between the first light transmission hole and the first detection unit . 12. The detection device according to claim 11, wherein the casing further has a second light-transmitting hole for receiving light around the casing. 13. The detection device according to claim 12, further comprising a second detection unit, the second detection unit is arranged at the mapping position of the second light transmission hole, to detect the light intensity around the housing, to generate the corrected value. 14. The detection device according to claim 13, wherein when the first detection unit detects the intensity of the light to be measured, the second detection unit also detects the intensity of light around the casing synchronously. 15. The detection device according to claim 1, wherein the first detection unit is a charge coupled device or a complementary metal oxide semiconductor. 16. A detection method for obtaining the concentration of a sample in a housing, comprising: obtaining a correction value, the correction value being related to light surrounding the housing; Obtain the intensity of the light to be measured according to the sample; calculating the intensity of the light to be measured and the correction value; and According to the calculation result, the concentration of the sample is obtained. 17. The detection method according to claim 16, wherein the correction value is obtained simultaneously or sequentially with the light to be measured. 18. The detection method according to claim 16, wherein the light to be measured is emitted by the sample.

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