CN101524150A - Microbial ecological agent and preparation method thereof - Google Patents
Microbial ecological agent and preparation method thereof Download PDFInfo
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- CN101524150A CN101524150A CN200910038538A CN200910038538A CN101524150A CN 101524150 A CN101524150 A CN 101524150A CN 200910038538 A CN200910038538 A CN 200910038538A CN 200910038538 A CN200910038538 A CN 200910038538A CN 101524150 A CN101524150 A CN 101524150A
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Abstract
The invention discloses a microbial ecological agent and a preparation method thereof. The microbial ecological agent mainly consists of the following raw materials based on the parts by weight: 5-50 of north caterpillar fungus reactants, 20-80 of lactic acid bacteria freeze-dried power, 5-20 of bifidus factors and 0.2-5 of vitamin; the north caterpillar fungus reactants contain 100g of north caterpillar fungus extract, 0.1-3g of alanine, 0.5-10g of glucose, 0.01-0.5g of ascorbic acid, 0-8g of sault, 0-2g of I+G and 0.001-0.01g of BHT. The microbial ecological agent is formed by preparing the north caterpillar fungus reactants, the lactic acid bacteria freeze-dried power and then mixing all the components, has multiple functions of digestion assistance, gastrointestinal disease prevention and cure, micro ecological balance maintenance and special health-care function of the north caterpillar fungus, and the like and has unique flavor and good taste. The preparation process is simple, the modern fermentation process is adopted, the north caterpillar fungus extract containing rich nutrition of amino acid, trace elements, and the like are added to fermentate and cultivate probiotics, and the number of fermentation liquid live bacteria is improved.
Description
Technical field
The invention belongs to biotechnology, relate to a kind of probiotics and preparation method thereof specifically.
Background technology
The north Chinese caterpillar fungus also claims Cordyceps militaris, is the abbreviation of northern Chinese caterpillar Fungus, has another name called northern Chinese caterpillar Fungus, silkworm chrysalis Cordyceps sinensis etc.After the nineties in 20th century, many scholars have carried out big quantity research to domestication and the pharmacological action of northern Chinese caterpillar fungus, confirm that the pharmacological action and the effect of north grass is identical with Cordyceps sinensis basically, can be used as the substitute of Cordyceps sinensis.Lung nourishing and qi invigorating arranged, increase smart kidney tonifying, hemostasis and phlegm, inhibition tumour, cough-relieving calmness, wind dispelling, heat radiation, stay Rong Meiyan, delay senility, tonifying kidney and strengthening yang, the reduction youth, effect and lung moistening and asthma relievings such as harmonious two sexual lifves, the enriching yin tonifying lung, enhance metabolism, improve the health, improve the effect of immunity of organisms.Can be widely used in industries such as bio-pharmaceuticals, effect type health products, cosmetics, flavouring.The northern Chinese caterpillar Fungus health products that gone on the market at present have: the aweto capsule of cordyceps militaris oral liquid, Chinese caterpillar fungus kidney-tonifying wine, Chinese caterpillar fungus health beer and Agricultural University Of Shenyang's development etc.Though the product of some northern Chinese caterpillar Fungus is arranged on the market, these product majorities rest on the roughing level, lack the market competitiveness, therefore, guarantee the development that the northern Chinese caterpillar Fungus industry can be continual and steady, just must improve the scientific and technological content of its converted products.In addition, what deserves to be mentioned is that in recent years, along with efficacy study and propaganda to northern Chinese caterpillar Fungus deepen continuously, foreign vendor had also produced keen interest to it, export volume constantly increases.And countries such as Japan and Korea S are in recent years all in the research and development of strengthening the Chinese caterpillar fungus functional food.
Along with the raising of living standards of the people and the enhancing of health perception, people more and more pay close attention to diet to organism physiology or otherwise influence and sanatory effect, and the propensity to consume is just turning to the food with adequate nutrition and health care.Because probio has good regulating action to body enteron aisle fungus strain, enteron aisle fungus strain balance then is to be related to human nutrition and healthy vital factor, and this makes the research of probio become focus in the functional food research gradually.Lactic acid bacteria is an important physical probio in the human body intestinal canal, has effects such as keeping the normal microecological balance of intestines and stomach, the invasion that suppresses pathogen and infection and enhancing immunity of organisms, is the main little ecological class raw material of plurality kinds of health care food and medicine.Commercial product has: sour milk, lactic acid bacteria tablet, lactic acid bacteria powder etc.Mei Shi in 1882 finds lactic acid bacteria, and day in 1971, the Benson factory that suckles forever produced the bifid sour milk, and beginning is sold in the European market.French ST.Hubert company in 1986, the Benson factory that suckles forever introduces production technology from day, and bifidobacterium yoghurt has a large circulation freely.20th century the eighties, China begins to produce.Oilgosaccharkdes is meant that oligosaccharides etc. is not digested and absorbs by the digestive ferment in the human body alimentary canal, but after entering human body intestinal canal, it can promote the growth of beneficial bacterium such as Bifidobacterium in the enteron aisle selectively, keep the general level of the health of human body, commercial product has: FOS, soyabean oligosaccharides, palatinose-oligosaccharides, oligoisomaltose etc.But do not see yet and utilize northern Cordyceps to promote lactobacillus-fermented, improve the zymotic fluid viable count, and northern Chinese caterpillar fungus and biodiasmin are organically combined the report that development has the two special health care compound health products concurrently.
According to searching document and database, detecting document related to the present invention has:
1, the research of northern Chinese caterpillar fungus commercialization cultivation technique (profound scholar friend etc., 2008).
2, the discarded compost Polysaccharide from Bee optimization research of northern Chinese caterpillar fungus (Ren Shuyu etc., 2008), the a large amount of discarded compost that the Chinese caterpillar fungus artificial cultivation produces, adopt orthogonal design, determine to be: extract 80 ℃ of temperature from the condition of extracting polysaccharide, extract extraction time 120min, solid-liquid ratio (w/v) 1: 20 2 times.Cleaner production and utilize resources synthetically that this experimental result can be northern Chinese caterpillar fungus provide reference.
3, active ingredient compares (Yang Qing etc. in the northern Chinese caterpillar fungus powder of two kinds of processing method acquisitions, 2008), purpose has detected cordycepin in the Chinese caterpillar fungus dry powder, thick polysaccharide, amino acid and vitamin equal size for the active ingredient of the northern Chinese caterpillar fungus dry powder of clear and definite freeze-drying and two kinds of processing methods acquisitions of oven dry.
4, the research and development of northern Chinese caterpillar fungus and active component thereof (Ni He etc., 2007).
5, northern Chinese caterpillar fungus batch production high-yield culture technique (Zhao Zhanchun etc., 2006).
6, compound north aweto capsule quality standard is inquired into (Liu Weimin etc., 2000).
7, the present Research of northern Chinese caterpillar fungus (Ge Beilei etc., 1999).
8, the research of northern Chinese caterpillar fungus resistant activity oxygen radical effect (Shen Qiying etc., 1997).
In document and the database retrieval scope, searching document has been introduced relevant northern Chinese caterpillar fungus at home.Mention the utilization of relevant cultivation, analysis of components, extract etc.But do not mention local flavorization.
Though northern Chinese caterpillar fungus, lactic acid bacteria and Oilgosaccharkdes be commercialization production and sales all, the function of these goods is all more single, and much the mouthfeel and the local flavor of health products do not obtain paying attention to.
Summary of the invention
One of purpose of the present invention provides a kind of probiotics, and this probiotics has plurality of health care functions, also has good mouthfeel and local flavor simultaneously.
The technical scheme that realizes above-mentioned purpose is as follows:
A kind of probiotics, it mainly is made up of the raw material of following weight portion:
North Chinese caterpillar fungus reactant 5-50 part, lactic acid bacteria freeze drying powder 20-80 part, Oilgosaccharkdes 5-20 part, and vitamin 0.2-5 part, described northern Chinese caterpillar fungus reactant consist of 100g north Cordyceps, alanine 0.1-3g, glucose 0.5-10g, ascorbic acid 0.01-0.5g, salt 0-8g, I+G (flavor enhancement) 0-2g and BHT (antioxidant) 0.001-0.01g.
The preferred group of described northern worm single reaction thing becomes: northern Cordyceps 100g, alanine 2g, glucose 5g, ascorbic acid 0.02g, salt 5g, I+G 0.5g and BHT0.002g.
Described northern Cordyceps is to be undertaken being concentrated into 5-20Brix (hundred sharp degree, i.e. solubility solid content) behind the enzymolysis by hydrolysis, the alkali protease that adds northern Chinese caterpillar fungus dry product quality 0.01-0.5% to obtain.More preferably, the alkali protease that adds northern Chinese caterpillar fungus dry product quality 0.02-0.2%.
Described Oilgosaccharkdes is one or more in trehalose, FOS, oligoisomaltose, soyabean oligosaccharides, palatinose-oligosaccharides, xylo-oligosaccharide, the oligomeric maltose.
The lactic acid bacteria freeze drying powder is by fermentation, centrifugal collections lactic acid bacteria mud in the zymotic fluid that is added with 0.1-5kg north Cordyceps in per 100 liters of conventional culture mediums, add the protective agent freeze drying then and must.Preferably, be added with 0.5-2kg north Cordyceps in per 100 liters of conventional culture mediums.
Another object of the present invention provides a kind of method for preparing above-mentioned probiotics.
A kind of method for preparing probiotics may further comprise the steps:
(1) preparation of northern Chinese caterpillar fungus reactant:
(1) adds its quality 5-40 water doubly in the Chinese caterpillar fungus dry product northwards, smash pulping after boiling, at 70-98 ℃ of following hydrolysis 1-6 hour; The pH of hydrolyzate is adjusted into 7.5-10, adds the alkali protease of northern Chinese caterpillar fungus dry product quality 0.01-0.5%, at 40-75 ℃ of following enzymolysis 4-12 hour; Filtration, vacuum are concentrated into 5-20Brix (hundred sharp degree, i.e. solubility solid content), promptly get northern Cordyceps;
(2) in the thickened pulp of every 100ml, add alanine 0.1-3g, glucose 0.5-10g, ascorbic acid 0.01-0.5g, salt 0-8g, I+G 0-2g, BHT0.001-0.01g, mix the back and give birth to fragrant reaction 3-8 hour at 60-100 ℃, adjusting total solid concentration is 10% spray-drying, get final product northern Chinese caterpillar fungus reactant;
(2) preparation of lactic acid bacteria freeze drying powder:
(3) cultivate lactic acid bacteria to the three grade fermemtation seed; Described lactic acid bacteria culturers be in bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium adolescentis, lactobacillus bulgaricus, lactobacillus acidophilus, Lactobacillus casei cheese subspecies, streptococcus thermophilus, the lactobacillus reuteri more than 2 kinds or 2 kinds;
(4) in conventional fermentation lactic acid bacterium culture medium, by adding 0.1-5kg north Cordyceps in per 100 liters of conventional culture mediums, accent pH is 5.0-7.5, sterilizes 15-40 minute for 100-121 ℃; When treating that culture medium is chilled to 10-35 ℃, three grades of seeds of every kind of lactic acid bacteria are pressed the inoculum concentration inoculation of the 2-20% (v/v) of culture medium cumulative volume, and single strain is lived or closed inoculation;
(5) technological condition for fermentation is: temperature 10-45 ℃, mixing speed 5-100r/min, stuffiness or anaerobism, tank pressure 0.01-0.10MPa, pH value maintain 5.0-7.0, time 8-24 hour; Refrigerated centrifuge is collected thalline after the fermentation ends;
(6) add the lactic acid bacteria protective agent by bacterium mud total amount, protective agent consists of: the 1-20% (w/w) of 5-20% (w/w) skimmed milk power of bacterium mud total amount, 2-10% (w/w) glycerine of bacterium mud total amount and bacterium mud total amount is selected from one or more materials in trehalose, FOS, oligoisomaltose, soyabean oligosaccharides, the palatinose-oligosaccharides; Bacterium mud behind the adding freeze drying protectant is mixed, carry out freeze drying then and get freeze-dried powder;
(3) product preparation
(7) northern Chinese caterpillar fungus reactant 5-50 part of weight portion, dry powder of lactic acid bacteria 20-80 part, Oilgosaccharkdes 5-20 part, and vitamin 0.2-5 part (as B family vitamin, vitamin C, vitamin D, vitamin E etc.) weighing, abundant mixing in Hi-mixer promptly gets described microecological health-care food then.
The present invention has the following advantages:
1. technology is simple; The warm plurality of health care functions of plurality kinds of health care product;
2. utilize nutritious northern Cordyceps of advanced enzyme engineering technology and Maillard reaction technology development and northern Chinese caterpillar fungus reactant with rich flavor;
3. by living fragrant reaction for preparation north Chinese caterpillar fungus reactant the time, utilize malic acid to make the product taste softer as the thermal response catalyst; Add palatinose and carry out thermal response, make the more mellow nature of northern Chinese caterpillar fungus local flavor as reduced sugar.
4. adopt modern zymotechnique, interpolation contains nutritious northern Cordyceps fermented and cultured probios such as amino acid, trace element, improves the zymotic fluid viable count;
5. utilize protease and give birth to fragrant reaction and develop northern Chinese caterpillar fungus reactant, then it is compositely gone into product, give good taste of product and nutrition;
6. go into the propagation that can promote beneficial bacterium in the enteron aisle in these health products on the one hand with Oilgosaccharkdes is compound, can promote the field planting of lactic acid bacteria in enteron aisle in these health products on the other hand.
7, adopt the scientific allocation technology, add nutriments such as northern Chinese caterpillar fungus thermal response thing, Oilgosaccharkdes, modulate unique flavor, good mouthfeel, nutritious, efficient probio active bacteria formulation with plurality of health care functions.
The specific embodiment
Embodiment one:
(1) northern Chinese caterpillar fungus reactant preparation:
1. the preparation of northern Cordyceps: get 2000 grams and do northern Chinese caterpillar fungus, add 40 premium on currency, pour beater into after boiling, pulled an oar 5 minutes, transfer pH to 9.5-10,95 ℃ of following hydrolysis 6 hours; The pH of hydrolysis slurry is adjusted into 8.5, adds the alkali protease of 4g, 60 ℃ of following enzymolysis 5 hours; It is northern Cordyceps that filtration, vacuum are concentrated into 10Brix;
2. northern Chinese caterpillar fungus reactant: in the northern Cordyceps that concentrates of every 100ml, add alanine 0.6g, glucose 3g, ascorbic acid 0.02g, BHT0.002g, mix the back and give birth to fragrant reaction 5 hours at 85 ℃, adjusting total solid concentration is 10% spray-drying, gets final product.
(2) preparation of active lactic acid bacteria preparation
1. lactobacillus acidophilus, lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium adolescentis are carried out actication of culture to three grade kind, culture medium is conventional lactic acid bacteria culture medium, and cultural method is conventional lactic acid bacteria cultivation;
2. with four 50 liters of fermentation tanks four kinds of bacterium are fermented respectively, every canned zymotic fluid 30-35 liter, per 100 liters of zymotic fluids consist of: 8 liters of Tomato juice, border peptone 0.5kg, glucose 2kg, yeastex 0.5kg, beef extract 1.0kg, sodium acetate 0.3kg, dipotassium hydrogen phosphate 200g, cysteine 20g, stir evenly the back and add 1kg north Cordyceps, mix the back and transfer pH to 6.5 with NaOH or hydrochloric acid, water is settled to 100 liters.In 115 ℃ of sterilizations 20 minutes, be chilled to 35 ℃, inoculation fermentation;
3. lactobacillus acidophilus, lactobacillus bulgaricus, Lactobacillus plantarum are inoculated respectively by 10% (v/v) inoculum concentration of its zymotic fluid cumulative volume (culture medium cumulative volume) respectively and carried out the stuffiness fermentation, tank pressure is kept with filtrated air; Bifidobacterium adolescentis carries out anaerobic fermentation (air in jar replaces with aseptic purity nitrogen) by 15% (v/v) inoculum concentration inoculation of zymotic fluid cumulative volume;
4. fermentation parameter: tank pressure 0.03MPa, mixing speed 30r/min, 37 ℃ of temperature, pH5.5-6.5; Fermentation time lactobacillus acidophilus, lactobacillus bulgaricus, Lactobacillus plantarum are 15 hours, and the bifidobacterium adolescentis fermentation time is 20 hours;
5. distinguish four kinds of thalline of centrifugal collection, the percentage by weight (w/w) of pressing bacterium mud total amount adds: 10% skimmed milk power, trehalose 8%, soyabean oligosaccharides 10%, glycerine 1%, mixing;
6. the thalline collected of freeze drying respectively, and grind sieve four kinds of pure lactic acid bacteria freeze drying bacterium powder.
(3) product preparation
1. by following prescription weighing: northern Chinese caterpillar fungus reactant 1000g, lactobacillus acidophilus bacterium powder 500g, streptococcus thermophilus bacterium powder 400g, lactobacillus bulgaricus bacterium powder 400g, bifidobacterium adolescentis bacterium powder 100g, oligoisomaltose 100g, FOS 50g, palatinose-oligosaccharides 50g, vitamin C 20g, vitamin E 10g;
2. with compound abundant mixing in the double helix Hi-mixer;
3. vacuum nitrogen is packed as finished product.
Embodiment two:
(1) northern Chinese caterpillar fungus reactant preparation:
1. the preparation of northern Cordyceps: get 10kg and do northern Chinese caterpillar fungus, add 250 premium on currency, pour beater into after boiling, pulled an oar 5 minutes, transfer pH to 9.5-10,85 ℃ of following hydrolysis 3 hours; The pH of hydrolysis slurry is adjusted into 8.5, adds the flavor protease of 15g, 60 ℃ of following enzymolysis 6 hours; It is northern Cordyceps that filtration, vacuum are concentrated into 12Brix;
2. northern Chinese caterpillar fungus reactant: in the northern Cordyceps of every 100g, add alanine 1.5g, glucose 5g, monosodium glutamate 3g, salt 2g, ascorbic acid 0.02g, BHT0.002g, mix the back and give birth to fragrant reaction 6 hours at 90 ℃, adjusting total solid concentration is 10% spray-drying, gets final product.
(2) preparation of active lactic acid bacteria preparation
1. lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium infantis and bifidobacterium adolescentis are carried out actication of culture to three grade kind, culture medium is conventional lactic acid bacteria culture medium, and cultural method is conventional lactic acid bacteria cultivation;
2. with two 500 liters of ferment tanks, fermentation tank is numbered No. 1 and No. 2, every canned zymotic fluid 350-400 liter, per 100 liters of zymotic fluids consist of: 8 liters of Tomato juice, border peptone 0.5kg, glucose 2kg, yeastex 0.5kg, beef extract 1.0kg, sodium acetate 0.3kg, dipotassium hydrogen phosphate 200g, cysteine 20g, stir evenly the back and add 2kg north Cordyceps, mix the back and transfer pH to 6.5, in 115 ℃ of sterilizations 20 minutes with NaOH or hydrochloric acid, be cooled to 35 ℃, wait to inoculate;
3. three grades of kinds of lactobacillus bulgaricus and streptococcus thermophilus are inoculated in No. 1 fermentation tank in 1: 1 ratio, total inoculum concentration is 10% (v/v) of zymotic fluid cumulative volume, adopts the stuffiness fermented and cultured, and tank pressure is kept with filtrated air;
4. three grades of kinds of bifidobacterium infantis and bifidobacterium adolescentis are inoculated in No. 2 fermentation tanks in 1: 1 ratio, total inoculum concentration is 15% (v/v) of zymotic fluid cumulative volume, adopts anaerobism to cultivate, and the air in jar replaces with aseptic purity nitrogen;
5. technological condition for fermentation: tank pressure 0.03MPa, mixing speed 50r/min, 37 ℃ of temperature, pH5.5-6.5,15 hours, No. 2 jar fermentation times of No. 1 jar fermentation time are 20 hours;
6. the thalline of difference No. 1 jar of centrifugal collection and No. 2 jars after the mixing of bacterium mud, is pressed percentage by weight (w/v) adding of bacterium mud total amount: skimmed milk power 10%, trehalose 10%, FOS 10%, glycerine 2%, mixing;
7. freeze drying thalline, and grind sieve compound freeze-dried lactic acid bacteria powder.
(3) product preparation
1. by following prescription weighing: northern Chinese caterpillar fungus reactant 2kg, compound lactobacillus freeze-dried powder 5kg, FOS 0.2kg, soyabean oligosaccharides 0.1kg, oligoisomaltose 0.3kg, palatinose-oligosaccharides 0.2kg, xylo-oligosaccharide 0.2kg, vitamin C 0.2kg, vitamin D 0.1kg, B family vitamin 0.2kg;
2. with compound abundant mixing in the double helix Hi-mixer;
3. vacuum nitrogen is packed as finished product.
Embodiment three:
(1) northern Chinese caterpillar fungus reactant preparation:
1. the preparation of northern Cordyceps: get 20kg and do northern Chinese caterpillar fungus, add 300 premium on currency, pour beater into after boiling, pulled an oar 5 minutes, transfer pH to 9.5-10,75 ℃ of following hydrolysis 4 hours; The pH of hydrolysis slurry is adjusted into 8.5, adds the alkali protease of 30g, 60 ℃ of following enzymolysis 5 hours; Filtration, vacuum are concentrated into 16Brix, promptly northern Cordyceps;
2. northern Chinese caterpillar fungus reactant: in the northern Cordyceps of every 100g, add alanine 0.8g, glucose 3g, monosodium glutamate 1g, salt 1.5g, ascorbic acid 0.02g, BHT0.002g, mix the back and give birth to fragrant reaction 7 hours at 75 ℃, adjusting total solid concentration is 10% spray-drying, gets final product.
(2) preparation of active lactic acid bacteria preparation
1. lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus reuteri are carried out actication of culture to three grade kind, culture medium is conventional lactic acid bacteria culture medium, and cultural method is conventional lactic acid bacteria cultivation;
2. at 1000 liters of canned zymotic fluid 750-800 liters of fermentation, per 100 liters of zymotic fluids consist of: 8 liters of Tomato juice, border peptone 0.5kg, glucose 2kg, yeastex 0.5kg, beef extract 1.0kg, sodium acetate 0.3kg, dipotassium hydrogen phosphate 200g, cysteine 20g, stir evenly the back and add 4kg north Cordyceps, mix the back and transfer pH to 6.5 with NaOH or hydrochloric acid, in 115 ℃ of sterilizations 20 minutes, be cooled to 35 ℃, wait to inoculate;
3. when being chilled to 38 ℃ lactobacillus bulgaricus, streptococcus thermophilus, three grades of kinds of lactobacillus reuteri are inoculated in 1000 liters of fermentation tanks in 1: 1: 1 ratio, total inoculum concentration is 10% (v/v) of zymotic fluid cumulative volume, adopt the stuffiness fermented and cultured, tank pressure is kept with filtrated air;
4. technological condition for fermentation: tank pressure 0.03MPa, mixing speed 15r/min, 37 ℃ of temperature, pH5.5-6.5, fermentation time 18 hours;
5. centrifugal collection thalline, the percentage by weight (w/w) of pressing bacterium mud total amount adds: skimmed milk power 10%, trehalose 3%, oligoisomaltose 5%, glycerine 5%, mixing;
6. freeze drying thalline, and grind sieve the compound lactobacillus freeze-dried powder.
(3) product preparation
1. by following prescription weighing: northern Chinese caterpillar fungus reactant 3kg, compound lactobacillus freeze-dried powder 5kg, FOS 0.5kg, soyabean oligosaccharides 0.2kg, oligoisomaltose 0.2kg, palatinose-oligosaccharides 1kg, vitamin C 0.3kg, B family vitamin 0.1kg;
2. with compound abundant mixing in the double helix Hi-mixer;
3. vacuum nitrogen is packed as finished product.
Techno-economic effect of the present invention:
It is active microbiological preparations that the present invention produces product, and its index detects with reference to lactic acid bacteria standard QB1554-1992 " sour milk beverage " and GB16321-2003 " sour milk beverage sanitary standard ".The every index result such as the following table of product:
Table 1 sense index
| Project | Index |
| Tissue morphology | Powdery |
| Color and luster | Be yellow to orange-yellow |
| Smell and flavour | Have the intrinsic fragrance of northern Chinese caterpillar fungus and characteristic flavor on basis, free from extraneous odour and stink |
| Impurity | The visible impurity of no naked eyes |
1.1 physical and chemical index
Should meet table 2 regulation.
Table 2 physical and chemical index
| Project | Index |
| Total arsenic (in As), mg/kg | ≤0.5 |
| Plumbous (in Pb), mg/kg | ≤1 |
| Food additives | Should meet GB 2760 regulations |
1.2 microbiological indicator
Meet table 3 requirement
Table 3 microbiological indicator
| Project | Index |
| Biodiasmin (cfu/g) | ≥10 8 |
| Coliform, MPN/100g | ≤30 |
| Pathogenic bacteria (salmonella, Shigella, staphylococcus aureus and hemolytic streptococcus) | Must not detect |
This product reaches the requirement of active bacteria formulation through the check viable count.Best in quality, the raciness of local flavor active microbiological preparations of the present invention's development, the product tool has the health care of northern Chinese caterpillar fungus and lactic acid bacteria concurrently, possesses the special bright fragrance with northern Chinese caterpillar fungus again simultaneously, belongs to high-grade health food.Research and develop this product good exemplary role is played in the development of the industry, will promote the development of functional food industry.
Claims (7)
1. a probiotics is characterized in that, it mainly is made up of the raw material of following weight portion:
North Chinese caterpillar fungus reactant 5-50 part, lactic acid bacteria freeze drying powder 20-80 part, Oilgosaccharkdes 5-20 part, and vitamin 0.2-5 part, consisting of of described northern Chinese caterpillar fungus reactant: northern Cordyceps 100g, alanine 0.1-3g, glucose 0.5-10g, ascorbic acid 0.01-0.5g, salt 0-8g, I+G 0-2g and BHT0.001-0.01g.
2. probiotics according to claim 1 is characterized in that, described northern Cordyceps is to be undertaken being concentrated into 5-20Brix behind the enzymolysis by hydrolysis, the alkali protease that adds northern Chinese caterpillar fungus dry product quality 0.01-0.5% to obtain.
3. probiotics according to claim 1; it is characterized in that; the lactic acid bacteria freeze drying powder is that 0.1-5kg north Cordyceps ferments, centrifugal collections lactic acid bacteria mud by being added with in per 100 liters of fermentation mediums, add the protective agent freeze drying then and must.
4. probiotics according to claim 1 is characterized in that, described Oilgosaccharkdes is one or more in trehalose, FOS, oligoisomaltose, soyabean oligosaccharides, palatinose-oligosaccharides, xylo-oligosaccharide, the oligomeric maltose.
5. a method for preparing probiotics is characterized in that, may further comprise the steps:
(1) preparation of northern Chinese caterpillar fungus reactant:
(1) adds its quality 5-40 water doubly in the Chinese caterpillar fungus dry product northwards, smash pulping after boiling, at 70-98 ℃ of following hydrolysis 1-6 hour; The pH of hydrolyzate is adjusted into 7.5-10, adds the alkali protease of northern Chinese caterpillar fungus dry product quality 0.01-0.5%, at 40-75 ℃ of following enzymolysis 4-12 hour; Filtration, vacuum are concentrated into 5-20Brix, promptly get northern Cordyceps;
(2) in the northern Cordyceps thickened pulp of every 100g, add alanine 0.1-3g, glucose 0.5-10g, ascorbic acid 0.01-0.5g, salt 0-8g, I+G 0-2g, BHT0.001-0.01g, mix the back and give birth to fragrant reaction 3-10 hour at 60-100 ℃, adjusting total solid concentration is 10% spray-drying, get final product northern Chinese caterpillar fungus reactant;
(2) preparation of lactic acid bacteria freeze drying powder:
(3) cultivate lactic acid bacteria to the three grade fermemtation seed; Described lactic acid bacteria culturers be in bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium adolescentis, lactobacillus bulgaricus, lactobacillus acidophilus, Lactobacillus casei cheese subspecies, streptococcus thermophilus, the lactobacillus reuteri more than 2 kinds or 2 kinds;
(4) in conventional fermentation lactic acid bacterium culture medium, by adding 0.1-5kg north Cordyceps in per 100 liters of conventional culture mediums, accent pH is 5.0-7.5, sterilizes 15-40 minute for 100-121 ℃; When treating that culture medium is chilled to 10-35 ℃, three grades of seeds of every kind of lactic acid bacteria are pressed the inoculum concentration inoculation of the 2-20% of culture medium cumulative volume, single strain or close inoculation;
(5) technological condition for fermentation is: temperature 10-45 ℃, mixing speed 5-100r/min, stuffiness or anaerobism, tank pressure 0.01-0.10MPa, pH value maintain 5.0-7.0, time 8-24 hour; Refrigerated centrifuge is collected thalline after the fermentation ends;
(6) add the lactic acid bacteria protective agent by bacterium mud total amount, protective agent consists of: the 1-10wt% glycerine of the 5-20wt% skimmed milk power of bacterium mud total amount, bacterium mud total amount and the 1-20wt% of bacterium mud total amount are selected from one or more materials in trehalose, FOS, oligoisomaltose, soyabean oligosaccharides, the palatinose-oligosaccharides; Bacterium mud behind the adding freeze drying protectant is mixed, carry out freeze drying then and get freeze-dried powder;
(3) product preparation
(7) northern Chinese caterpillar fungus reactant 5-50 part of weight portion, dry powder of lactic acid bacteria 20-80 part, Oilgosaccharkdes 5-20 part, and vitamin 0.2-5 part, weighing, abundant then mixing promptly gets described microecological health-care food.
6. preparation method according to claim 5 is characterized in that, in the step (1), the quality that adds alkali protease is the 0.02-0.2% of northern Chinese caterpillar fungus dry product quality.
7. preparation method according to claim 5 is characterized in that, in the step (4), by adding 0.5-2kg north Cordyceps in per 100 liters of conventional culture mediums.
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| CN200910038538A Expired - Fee Related CN101524150B (en) | 2009-04-10 | 2009-04-10 | Microbial ecological agent and preparation method thereof |
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