CN101375915A - Preparation of Rhodiola rosea glycosides and application thereof in medicine field - Google Patents
Preparation of Rhodiola rosea glycosides and application thereof in medicine field Download PDFInfo
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- CN101375915A CN101375915A CNA2008101207526A CN200810120752A CN101375915A CN 101375915 A CN101375915 A CN 101375915A CN A2008101207526 A CNA2008101207526 A CN A2008101207526A CN 200810120752 A CN200810120752 A CN 200810120752A CN 101375915 A CN101375915 A CN 101375915A
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- radix rhodiolae
- lixiviate
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- rhodioside
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Abstract
The invention discloses a technique for extracting salidroside from Rhodiola sachalinensis belonging to Crassulaceae. The technical process is as follows: a prepared raw material of Rhodiola rosea is dipped in warm water at 35 DEG-40 DEG for 2 hours, a beating machine is used for beating, the obtained Rhodiola rosea liquid is added with purified water of 6 times weight and then pumped into a vacuum extraction container, the filtration is carried out after 12 hours of hot-extraction, the filtrate is put into a cooling tank for cooling, and the salidroside can be obtained after single-action concentration and spray drying. The invention has the characteristics of simple technique, step-by-step operation, low cost and high product purity, thereby being suitable for industrialization.
Description
Technical field
The invention belongs to medical technical field, be specifically related to a kind of from Radix Rhodiolae extraction separation obtain rhodioside and the treatment tumor medicine in application.
Background technology
Radix Rhodiolae (Rhodiola) is Crassulaceae rhodiola renascent herb or semishrub plant, and Tibetan language is referred to as " Suo Luomabu ", is described as " plateau Radix Ginseng ", and the Radix Rhodiolae great majority all are grown under height above sea level 3500～5000m left and right sides high mountain flowstone or the scrub forest.The physiologically active ingredient of Radix Rhodiolae uniqueness and strong pharmacological effect are paid close attention to by common people deeply, and the classical masterpiece Four-Volume Medical Code of Tibetan medicine and " brilliant pearl book on Chinese herbal medicine " are all on the books to Radix Rhodiolae.
Cough due to lung-heat, hemoptysis, leucorrhea, the traumatic injury etc. of being used for the treatment of among the people.Rhodiola tibetica (Hook. f. et Thoms.) S. H. Fu medicinal plants kind is a lot of, and long whip Radix Rhodiolae, Xima rhodiola, knuckle-tooth Radix Rhodiolae, full lobe Radix Rhodiolae, back of the body medicine Radix Rhodiolae etc. are arranged.Radix Rhodiolae has the effect of strengthening the body resistance, YIN and YANG balance regulating, vigorate qi and replenish the blood, freeing vessels and nourishing heart, nourishing the brain and improving intelligence, nourishing and fit keeping function.Pharmaceutical research shows: rhodiola rosea formulated product has defying age, resisting fatigue, antiviral, anti-hypoxia, resisting microwave radiation effect.
Summary of the invention
The objective of the invention is, from Radix Rhodiolae, extract its effective ingredient, and make industrialization, can be used as the raw material of preparation medicine for treating tumor thing.
The Radix Rhodiolae extraction process is as follows: with the Radix Rhodiolae raw material of getting ready, with 35 ℃～40 ℃ warm water soaking 2 hours, the beater making beating, the gained rhodiola liquid pumps into vacuum lixiviate filling after adding purifying waste water of 6 times of weight, heat is carried 12 hours and is filtered then, and filtrate changes the cooling tank cooling over to, and single-action concentrates, enter spray drying again, promptly get rhodioside.
The present invention has carried out antitumor activity in vitro to rhodioside, shows that it has the effect of obvious suppression tumor.
1, experiment purpose:
Influence to the growth of tumor cell lines such as the strain of Hela human cervical carcinoma cell, the strain of Bcap-37 human breast cancer cell, HO8910 human oophoroma cell line, T24 human bladder cancer cell strain.
2, method:
Test method is a tetramethyl azo azoles salt method: cell experiment divides Radix Rhodiolae extract 0.1,0.01,0.001,4 concentration groups of 0.0001g/mL, negative control group (culture fluid that contains same cell density), solvent control group (75g/L glycerol).(cell is 5 * 10 to the exponential phase cell
8L
-1) 100 μ L are inoculated in 96 orifice plates, 37 ℃ of saturated humidities, volume fraction are 0.05 CO
2Under the condition, cultivate 4h, treat that cell is all adherent, add the thing that tried of 100 μ L various dose, respectively establish 5 multiple holes.24,48,72h takes out 96 orifice plates respectively, and after inverted microscope was observed down, every hole added 5g/L tetramethyl azo azoles saline solution 20 μ L, and 37 ℃ are continued to hatch 4h, stop cultivating, and careful the suction abandoned culture supernatant in the hole.Every hole adds dimethyl sulfoxine 150 μ L, and vibration 10min then, selects 578nm, measures each hole absorbance on enzyme-linked immunosorbent assay instrument, the record result.
3, result: as shown in table 1 below.
Table 1 Radix Rhodiolae extract is to the influence of growth of tumour cell
Compare with negative control group,
aP＜0.05,
bP＜0.01
Table 1 is the result show, each concentration of rhodioside all has inhibitory action to the strain of Bcap-37 human breast cancer cell, HO8910 human oophoroma cell line's growth.Radix Rhodiolae extract concentration is 0.001～0.1g/mL has inhibitory action to the growth of the strain of Hela human cervical carcinoma cell, T24 human bladder cancer cell strain, and concentration is 0.0001g/mL unrestraint effect.
The specific embodiment
Embodiment 1
With 50 kilograms of the Radix Rhodiolae got ready grass, with 35 ℃～40 ℃ warm water soaking 2 hours, send into beater afterwards and pull an oar, the gained rhodiola liquid pumps into vacuum lixiviate filling after adding purifying waste water of 6 times of weight, and heat is carried 12 hours and filtered then, filtrate changes the cooling tank cooling over to, single-action concentrates, and enters spray drying again, promptly gets 11.9 kilograms of rhodiosides, yield 23.8%, finished product packing.
Embodiment 2
Get rhodioside 100g, disperse with an amount of water for injection, add right amount of auxiliary materials, mixing is made granule, spray drying.
Press the granule of embodiment gained, be sub-packed in the hungry area shell, promptly get capsule by required dosage.
Press the granule of embodiment gained, direct compression, film coating promptly gets tablet.
Be used for the treatment of tumor.
Embodiment 3
Get rhodioside 400mg, add the injection water and dissolve in right amount.With mannitol 400mg,, promptly get the Radix Rhodiolae lyophilized powder according to conventional freeze-dry process lyophilizing.Be used for the treatment of tumor.
Claims (7)
1, a kind of from Radix Rhodiolae high efficiency extraction separate and to have the method for anti-tumor active substance rhodioside, it is characterized in that the Radix Rhodiolae grass that to get ready, with 35 ℃～40 ℃ warm water soaking 2 hours, beater making beating, gained rhodiola liquid pump into vacuum lixiviate filling after adding purifying waste water of 6 times of weight, and heat is carried 12 hours and filtered then, filtrate changes the cooling tank cooling over to, single-action concentrates, and enters spray drying again, promptly gets rhodioside.
2, by the described Radix Rhodiolae of claim 1, it is characterized in that Radix Rhodiolae is a kind of Radix Rhodiolae section Rhodida plant that is distributed in Northern Hemisphere severe cold areas
3, get the raw materials ready by claim 1 is described, it is characterized in that, raw material is handled, satisfy the granularity of lixiviate needs, the qualified and raw material that purifies improves extraction rate and product quality to reach, and cuts down the consumption of raw materials.
4, irritate lixiviate by the described lixiviate of claim 1, it is characterized in that, the operating pressure that lixiviate is irritated is formed by hydraulic pressure, allow and use 120 ℃ of higher extraction temperatures, production capacity and extraction rate are higher, and the extraction time of Radix Rhodiolae is about 12 hours, and the fluid coefficient is up to 600～500%.Easy to operate, labor intensity is little, and equipment life is long, for ease of maintenaince.
5, concentrate by the described single-action of claim 1, it is characterized in that, adopt outer heating Natural Circulation and negative pressure of vacuum to evaporate to combine mode, evaporation rate is fast, cocnentration factor can reach 1.3, the liquid material concentrates in the whole sealing status non-foam, with the medicinal liquid that haplo-effect concentrator concentrates out, have pollution-free, the characteristics that flavour of a drug are dense, and clean conveniently, open the upper and lower covers of heater and can clean.Haplo-effect concentrator is simple to operate, and floor space is little.Heater, vaporizer adopt the rustless steel insulation construction, and heat-insulation layer external stainless sheet steel is made shell, and minute surface or inferior optical processing are done in the surface.
6, by the described spray drying of claim 1, it is characterized in that the material of ejection just just is subjected to high temperature when the atomisation size particles,, can keep rhodioside after drying, still to keep its active ingredient and not be damaged so be to be heated moment.
7, by the application of the described Radix Rhodiolae extract of claim 1 in preparation medicine for treating tumor thing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101207526A CN101375915A (en) | 2008-09-11 | 2008-09-11 | Preparation of Rhodiola rosea glycosides and application thereof in medicine field |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008101207526A CN101375915A (en) | 2008-09-11 | 2008-09-11 | Preparation of Rhodiola rosea glycosides and application thereof in medicine field |
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| CN101375915A true CN101375915A (en) | 2009-03-04 |
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| CNA2008101207526A Pending CN101375915A (en) | 2008-09-11 | 2008-09-11 | Preparation of Rhodiola rosea glycosides and application thereof in medicine field |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012041256A1 (en) * | 2010-09-30 | 2012-04-05 | Chi-Ying Huang | Anti-cancer extract and compounds |
| CN103012512A (en) * | 2013-01-16 | 2013-04-03 | 河北科技大学 | Separation and purification method of salidroside in natural rhodiola sachalinensis |
| CN103933054A (en) * | 2014-04-13 | 2014-07-23 | 徐鹏 | Cyclophosphamide-containing pharmaceutical composition and application in treatment for breast cancer |
| CN105832780A (en) * | 2016-05-25 | 2016-08-10 | 朱国平 | Medicinal composition capable of enhancing human body immunity and having anti-cancer effect and preparation method thereof |
-
2008
- 2008-09-11 CN CNA2008101207526A patent/CN101375915A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012041256A1 (en) * | 2010-09-30 | 2012-04-05 | Chi-Ying Huang | Anti-cancer extract and compounds |
| CN103269706A (en) * | 2010-09-30 | 2013-08-28 | 黄奇英 | Anticancer Extracts and Compounds |
| CN103269706B (en) * | 2010-09-30 | 2015-06-24 | 黄奇英 | Anticancer Extracts and Compounds |
| CN103012512A (en) * | 2013-01-16 | 2013-04-03 | 河北科技大学 | Separation and purification method of salidroside in natural rhodiola sachalinensis |
| CN103012512B (en) * | 2013-01-16 | 2016-03-30 | 河北科技大学 | The separation purification method of rhodioloside in neutral red red-spotted stonecrop |
| CN103933054A (en) * | 2014-04-13 | 2014-07-23 | 徐鹏 | Cyclophosphamide-containing pharmaceutical composition and application in treatment for breast cancer |
| CN103933054B (en) * | 2014-04-13 | 2016-01-20 | 徐鹏 | A kind of pharmaceutical composition containing cyclophosphamide and the application in treatment breast carcinoma |
| CN105832780A (en) * | 2016-05-25 | 2016-08-10 | 朱国平 | Medicinal composition capable of enhancing human body immunity and having anti-cancer effect and preparation method thereof |
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Open date: 20090304 |