CN100575927C - Resonance imager of light-exciting color surface piasma - Google Patents
Resonance imager of light-exciting color surface piasma Download PDFInfo
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- CN100575927C CN100575927C CN200610011926A CN200610011926A CN100575927C CN 100575927 C CN100575927 C CN 100575927C CN 200610011926 A CN200610011926 A CN 200610011926A CN 200610011926 A CN200610011926 A CN 200610011926A CN 100575927 C CN100575927 C CN 100575927C
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Abstract
A kind of resonance imager of light-exciting color surface piasma mainly is made up of light source, incident angle of light modulating system, sample analysis system, temperature control system, detection system, data acquisition management system.This instrument with polychromatic light as excitation source, surface plasma that utilizes the total reflection medium to be coupled to excite sensing membrane and resonance with it, the purpose that generation colour (the comprise black and white) image relevant with laboratory sample character, and then realization thus analyzed and detected sample.The present invention both can carry out list and plant sample analysis, can also carry out microarray to sensing membrane and handle, and formed colour optics chip imaging technique, realized high flux, the multiple spot parallel analysis of sample.The variation of caused colored resonance image per sample, this instrument not only can be carried out qualitative and quantitative analysis to various biomolecule and abiotic sample component, but also Real-time and Dynamic study sample character and bio-molecular interaction.
Description
Technical field
The present invention relates to surface plasma body resonant vibration, colour imaging principle, metal surface dynamic monitoring and two-dimensional lattice chip imaging technique relate in particular to a kind of resonance imager of light-exciting color surface piasma.
Technical background
Surface plasma body resonant vibration (Surface Plasmon Resonance) is called for short SPR, is a kind of physical optics phenomenon.When a branch of plane p-polarized light incides the interface of metal film close-connected with it and low refractive index dielectric through the glass of high refractive index medium, in incident angle or wavelength is under the condition of a certain desired value, surface plasma is identical with incident light disappearance wave frequency and wave number, the two will resonate, incident light is absorbed, energy of reflection light sharply descends, and intensity of reflected light reaches minimum.At this moment incident angle, incident wavelength are called as resonance angle and resonant wavelength respectively.Because resonance angle or resonant wavelength are very responsive to medium (sample) change of refractive that abuts against the metallic film surface, based on this principle, the difference of refractive index properties can realize the real-time and harmless light of sample is detected per sample.Do not need purifying, biological sample to need not characteristics such as mark, sensitivity are higher, no background interference because the SPR technology has real-time monitoring reaction dynamic process, analytic sample, in the bio-science field application, have incomparable advantage.
Along with the enforcement of the develop rapidly of biotechnology, particularly human genome and proteomic program, a large amount of biological informations needs high flux and the analytical technology that can monitor in real time detects.In order to improve analysis throughput and analysis speed, international and domestic advantage and microarray high-throughput techniques with SPR combines, and carried out the imaging research based on the SPR principle, i.e. SPR imaging, existing at present patent report.By making the chip of microchannel and micro array structure, sample analysis ability and speed are all improved greatly.Yet current SPR imager is only monitored the variation (gray-scale value) of single wavelength intensity of reflected light, or is called for short single wavelength black and white SPRI.This method is simple, but has only a kind of pattern of angular adjustment.Being used for the SPR imaging for multi-wavelength and wave band, one piece of document is only arranged, is the research about ultra-thin organic solid film character, and only touch upon arrives qualitative analysis.Up to the present, Shang Weijian has any patent report.
Summary of the invention
The purpose of this invention is to provide a kind of surface plasma resonance image-forming detector, change single wavelength SPR and be imaged as multi-wavelength and wave band SPR imaging, to obtain and abundanter sample experiment information is provided, make the easier analysis and judgement of experimental result, experiment detect hommization more simultaneously.
For achieving the above object, technical solution of the present invention is to ask survey surface plasma body resonant vibration principle based on wavelength, in conjunction with the coloured image recording technique, and the system that makes up the generation of surface color signal, record, storage, processing and reproduce.In view of the above development and design a kind of multi-functional resonance imager of light-exciting color surface piasma, but adopt angle-wavelength conjugation double adjustment method to increase the parameters optimization of instrument, realize the variation of color after the sample imaging of different nature, made more horn of plenty of reaction information.Instrument mainly is made up of light source, incident angle of light modulating system, sample analysis system, temperature control system, detection system, data acquisition management system.
In detail, resonance imager of light-exciting color surface piasma provided by the invention mainly is made up of light source, incident angle of light modulating system, sample analysis system, temperature control system, detection system, data acquisition management system; Wherein:
One sample analytic system, its both sides are provided with a continuous wave incident light and a reflected light detector respectively; This reflected light detector connects a data acquisition management system;
The sample cell both sides are provided with the sample feeding pipeline and go out the sample pipeline in the sample analysis system, and a micro pump communicates by sample introduction pipeline and sample cell and controls the flow velocity and the flow of sample introduction; The sample cell two ends are fixed on the horizontal stand, the pond body down, with the film of sensing membrane surface washer sealing, a sensing membrane bottom surface and a prism back side adhesion; The surface of sensing membrane has the high electricity of free electron layer and leads material;
The irradiation of continuous wave incident light excites the sensing membrane surface plasma to produce resonance by prism, directly causes catoptrical color to change.The reflected light that penetrates from prism enters detection system, and is transferred to data acquisition management system by electric signal and carries out data processing, realizes qualitative and quantitative analysis.
Described resonance imager of light-exciting color surface piasma, wherein incident light is injected the sample analysis system through an incident angle of light regulating system, and this incident light regulating system is that an optical mirror is fixed on the mirror support of an adjustable angle.
Described resonance imager of light-exciting color surface piasma, wherein incident light is white light or the polychromatic light that halogen tungsten lamp, xenon lamp, Nernst glower, Elema lamp or light emitting diode send, and modulates through an optical beam expander or beam compressor.If incident light is a nonpolarized light, need before optical beam expander or beam compressor, to add the p-type polarized light polarizer.
Described resonance imager of light-exciting color surface piasma, wherein prism is that inorganic optical glass, chemosynthesis optical resin plastics or tool light transmission fluent material are made.
Described resonance imager of light-exciting color surface piasma, wherein reflected light detector is colored CCD (charge-coupled device), color cmos (complementary metal oxide semiconductor (CMOS)) imageing sensor, charge-injection detector(CID), photomultiplier or camera.As the lens of reflected light Deviation rectifier and its with optical axis be fixed on the runing rest, form detection system, reflected light is proofreaied and correct and focused.
Described resonance imager of light-exciting color surface piasma, wherein the body top, pond of the sample cell in the sample analysis system is connected with the heat dissipation pipeline of a temperature control system, this heat dissipation pipeline connects a cooling liquid container, and this cooling liquid container connects the temperature control circuit of a temperature control regulator.
Described resonance imager of light-exciting color surface piasma, wherein sensing membrane is golden film, silverskin or aluminium film, thickness is 10-200nm.
Described resonance imager of light-exciting color surface piasma, wherein data acquisition management system is used to finish tasks such as the analyzing and processing of the monitoring of signal and storage, data and experimental result output.
Described resonance imager of light-exciting color surface piasma, wherein any position configuration monochromator or utilize the monochromatic light electric transducer before the detecting device promptly is convertible into black and white surface plasma resonance image-forming instrument.
Described resonance imager of light-exciting color surface piasma, wherein any position configuration dispersion system before the detecting device can change wavelength into and ask the surface plasma resonance optical spectrum instrument of surveying.
Of the present invention have a following novelty:
The dynamic change of image color (very color rather than pseudo-color) is used for the real-time monitoring and qualitative, the quantitative test of surface plasma resonance image-forming, the more abundant informationization that makes experiment, make the use hommization more of instrument, this is topmost advantage and innovative point that instrument of the present invention makes up.
Multifunctionality of the present invention:
A, imaging color change with the variation of properties of samples, substitute the variation of single color gray-scale value with colored variation, have strengthened the contrast and the resolvability of experimental image;
B, employing angle-wavelength conjugation double adjustment method are realized the optimization of experiment condition;
C, can be chosen to the control of picture scheme and image, realize the optimization of image local or Global Information according to the experiment needs;
D, can be made into the biological and chemical probe, carry out the high flux of several samples, parallel performance analysis, develop into the optical biochip technology;
E, can realize the mutual conversion of coloured image and spectral line also can being converted into concrete numerical value to image information, carry out qualitative and quantitative analysis;
The field of f, detection is wide, and object is wide.Be applicable to the detection in fields such as biology, clinical medicine, medicine, chemistry and physics; The object of analyzing from albumen, nucleotide, DNA, RNA sugar, lipid to systems such as inorganic ions, bacteriophage, virion, cells; Real-time dynamic monitoring and Non-Destructive Testing can be accomplished, also qualitative and quantitative analysis can be carried out; And experimental phenomena is very obvious, uses simply, and experimental result is accurately understandable.Can provide a kind of high flux, cold detection technique platform for functional gene research and proteome research.
Structure flexibility and changeability of the present invention:
A, light-source system alternative are by force.Can adopt polychromatic light light sources such as white light or multi-wave length illuminating diode, and polarization and nonpolarized light can use all;
B, employing optical element and physical construction minute adjustment are optimized, and accurately control the angle of incident light and the angle of detection system, can be reduced to the distortion of picture and the sharpness of maintenance image;
C, sensing membrane can adopt several different methods preparation and surface construction;
D, sample pool structure are simple, make easily.Can design and produce into hyperchannel, as reference and parallel comparative analysis;
The extensibility of e, whole instrument system is strong.By the modified segment element, can be converted to single wavelength table surface plasma resonance imaging system and surface plasma resonance optical spectrum instrument.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Fig. 2 and Fig. 3 are respectively the synoptic diagram of light source A among Fig. 1;
Fig. 4 is the synoptic diagram of incident angle of light regulating system B among Fig. 1;
Fig. 5 is the synoptic diagram of the C of sample analysis system, temperature control system D among Fig. 1;
Fig. 6 is the synoptic diagram of detection system E among Fig. 1;
Fig. 7 handles and the data analysis software schematic flow sheet for the picture editting of resonance imager of light-exciting color surface piasma of the present invention;
Fig. 8 is the control system program circuit synoptic diagram of resonance imager of light-exciting color surface piasma of the present invention;
Fig. 9 is the software flow pattern of resonance imager of light-exciting color surface piasma of the present invention;
Figure 10 is the colour imaging figure of the immunoglobulin G microarray of multi-functional resonance imager of light-exciting color surface piasma mensuration;
Figure 11 is the colour imaging figure of insulin microarray in aqueous solution that multi-functional resonance imager of light-exciting color surface piasma is measured;
The immunoglobulin G microarray that Figure 12 measures for multi-functional resonance imager of light-exciting color surface piasma the linear spectral line chart that is converted into by working out software certainly of colour imaging figure;
Figure 13 askes the surface plasma body resonant vibration spectrum of surveying for the interactional wavelength of Instrument measuring antigen of the present invention (immunoglobulin G while) antibody (the anti-immunoglobulin G while of goat anti-human immunoglobulin G and rabbit).
Embodiment
Resonance imager of light-exciting color surface piasma essential structure of the present invention is: on a platform, the sample analysis system is set; Left side and right side in the sample analysis system independently set into shooting angle modulating system and detection system respectively; Near the sample cell of sample analysis system, connect temperature control system is set; Rear at the incident angle of light modulating system independently is provided with light source; Right side in detection system is electrically connected data acquisition management system.Each system of instrument is modular design, and except data acquisition management system, other parts place in the shell.Light channel structure is: the ray cast that light source sends is to the optical mirror of incident angle of light modulating system, height refraction light transmissive medium after angular modulation in the light penetration sample analysis system shines the sensing membrane surface, on the sensing membrane surface total reflection takes place, light reflected penetrates high refraction light transmissive medium and enters detection system at last.
Resonance imager of light-exciting color surface piasma of the present invention, its described coloured image is the rgb image of the real-time detected sample of instrument, but not the pcolor picture that after any technological means (comprising computer software) is handled, obtains.
Resonance imager of light-exciting color surface piasma of the present invention, any possible position adds monochromator before detecting device, or utilizes the monochromatic light electric transducer, promptly is convertible into black and white surface plasma resonance image-forming instrument.
Resonance imager of light-exciting color surface piasma of the present invention, any possible position configuration dispersion system can change wavelength into and ask the surface plasma resonance optical spectrum instrument of surveying before detecting device.
Resonance imager of light-exciting color surface piasma of the present invention, its described light source, exemplary light sources is that directional light, point-like or spherical light source can be modulated by beam spread (or compression) device or similar optical system.For nonpolarized light, need add the p-type polarized light polarizer.
Resonance imager of light-exciting color surface piasma of the present invention, its described light source comprises any polychromatic light light sources such as halogen tungsten lamp, xenon lamp, Nernst glower, Elema lamp and light emitting diode.
Resonance imager of light-exciting color surface piasma of the present invention, its described polarized light polarizer comprises polymkeric substance polaroid, birefringent polarizing prism or wave-plate stack polarizer etc.Polarizer can place any possible position before the detecting device.
Resonance imager of light-exciting color surface piasma of the present invention, its described incident angle of light regulating system is made up of optical mirror and angle demodulator.Wherein optical mirror is connected with the axis of rotation of angle demodulator.The angle rotation of angle demodulator linkage optical mirror, thereby the meticulous adjusting of realization incident angle of light.
Resonance imager of light-exciting color surface piasma of the present invention, form such as the angle of its described incident light can accurately be regulated by the incident angle of light regulating system, and it is automatic, semi-automatic and manual that mode comprises.The range of choice of angle can be set according to the experiment needs, and angular adjustment resolution is determined by angle demodulator.
Resonance imager of light-exciting color surface piasma of the present invention, its described temperature control system is made up of liquid coolant, radiating tube, temperature sensor and temperature control circuit.The liquid coolant sample cell inside of flowing through, the temperature of sample analysis system is accurately regulated and control by liquid coolant.Both can realize constant temperature, also can temperature programme.
Resonance imager of light-exciting color surface piasma of the present invention, its described sample analysis system is made up of sample delivery and electromagnetic wave coupling sensor two parts.
Wherein the sample delivery part is made up of sample flow path system and micro pump.Sample feeding pipeline and go out the both sides that the sample pipeline lays respectively at sample cell, and communicate with the two ends of sample cell pond body.Micro pump communicates by sample introduction pipeline and sample cell, by setting the pressure indicator of regulating micro pump, can control the flow velocity and the flow of sample introduction.Sample introduction and stream clean and can manually control, also can be automatically, semiautomatic control.
Wherein electromagnetic wave coupling sensor part is made up of sample cell, sensing membrane, high refraction light transmissive medium and locator card.The sample cell two ends are fixed on the horizontal stand, the pond body down, with the film surface washer sealing of sensing membrane.Sensing membrane bottom surface and the liquid adhesion of high refraction translucent material medium.Locator card is used for fixing the high refraction of high refraction light transmission light transmissive medium.
Resonance imager of light-exciting color surface piasma of the present invention, the material of its described high refraction light transmissive medium comprises materials such as inorganic optical glass and chemosynthesis optical resin and high medium light transmission liquid.Its described sample cell can use biochemical inert material such as teflon, silicon rubber to make.
Resonance imager of light-exciting color surface piasma of the present invention, its described sensing membrane are that the surface has the high electricity of free electron layer to lead material, comprise metal films such as golden film, silverskin, aluminium film.
Sensing membrane can be by the preparation of methods such as vacuum evaporating, plating and plated film, and thickness is in 10-200nm.
Resonance imager of light-exciting color surface piasma of the present invention, its described sensing membrane can be constructed micro probe array by chemistry or bio-modification, forms biochip.The identification, detection, high flux screening and the parallel analysis that are used for various target molecules.
Resonance imager of light-exciting color surface piasma of the present invention, its described detection system is made up of reflected light Deviation rectifier, detecting device, angle demodulator.Reflected light Deviation rectifier and detecting device mechanical connection be at same horizontal level, and place on the angle demodulator by extension bar.Angle demodulator can be realized the accurate adjusting of detection system angle, both can manual adjustments, and also can automatic, semi-automatic adjusting.
Resonance imager of light-exciting color surface piasma of the present invention, its described reflected light Deviation rectifier can be proofreaied and correct and optical focusing reflected light, makes its blur-free imaging on the focal plane of detecting device.Its described detecting device can be to have response also can realize the sensor or the image detection registering instrument of dynamic color imaging to multi-wavelength light, comprises colored CCD, color cmos, charge-injection detector(CID), photomultiplier, photographic system etc.
Resonance imager of light-exciting color surface piasma of the present invention, the angle demodulator of its described incident angle of light system and the angular adjustment of detection system can synchro control, also independent regulation separately.
Resonance imager of light-exciting color surface piasma of the present invention, its described data acquisition management system is used to finish the collection and the tasks such as analyzing and processing and experimental result output of the monitoring of signal and storage, data.Form by computing machine, image pick-up card, picture editting's processing and data analysis software.Wherein, image pick-up card carries out real-time collection, demonstration, storage and the output of image.The picture editting handles and data analysis software is carried out off-line analysis to image, choosing at random and the real-time optimization function of integral body, topography's collection can be provided, the signal comparison in the two dimensional image and the conversion of image and spectral line be can carry out, the function optimization regulation and control of image and the dynamic process of monitoring sample spot according to the variation of picture signal in real time realized.Also the color of image can be converted into absolute figure according to funtcional relationship, sample is carried out qualitative and quantitative analysis.
Be illustrated below in conjunction with the detection of accompanying drawing apparatus of the present invention and concrete sample.
The apparatus example
The present invention is a multifunctional light excitating surface plasma resonance imager, mainly is made up of light source A, incident angle of light modulating system B, the C of sample analysis system, temperature control system D, detection system E, data acquisition and management system F.Fig. 1 is a kind of with the basic structure exemplary plot of prism as coupled mode of multi-functional resonance imager of light-exciting color surface piasma.
The basic structure of light source A mainly is made up of excitation source, optical beam expander as shown in Figure 2.Optical beam expander can be the commercialization optical element, also can self manufacture.Excitation source and optical beam expander both can be installed separately, also can overall package in parallel light tube, the distance between light source and the optical beam expander can be regulated, but their axle center is consistent.Light source comprises parallel p-polarization polychromatic light light source 21, optical beam expander (lens 22, diaphragm 23, lens 24).
Fig. 3 is the light channel structure exemplary plot of wherein a kind of spherical white light of the multi-functional resonance imager of light-exciting color surface piasma of the present invention.Main spherical white light source 31, diaphragm 32, optical beam expander (lens 33, diaphragm 34, lens 35), polaroid 36 are formed.
The basic structure of incident angle of light regulating system B as shown in Figure 4.The incident angle of light regulating system mainly comprises optical mirror 41 and angle demodulator.Angle demodulator can be a universal stage, manually angle modulation, the also automatically controlled angle modulation of stepper motor.It is configured to: optical mirror 41 is fixed on the mirror support 42, and extension bar 43 1 ends connect mirror support 42, and the other end is fixed on the screw on the axis of rotation of universal stage 44, the angle of regulating universal stage, the angle rotation of the optical mirror that can link.When the beam level of light source ejaculation shines on the optical mirror, regulate the micrometric screw of universal stage, catoptrical angle changes with the angle of optical mirror, thereby realizes the meticulous adjusting of incident angle of light.
The basic structure of C of sample analysis system and temperature control system D as shown in Figure 5.Wherein the sample analysis system mainly is made up of sample delivery and electromagnetic wave coupling sensor two parts.The sample delivery part is mainly become by micro pump and relevant stream set of systems.Sample feeding pipeline 53 and go out the both sides that sample pipeline 54 lays respectively at sample cell 56, and communicate with the two ends of sample cell pond body.One end of micro pump 52 is connected with sample reservoir 51, and the other end communicates by sample introduction pipeline 53 and sample cell 56, by setting the vacuum indicating gauge of regulating micro pump 52, can control the flow velocity and the flow of sample introduction.Go out sample pipeline 54 and connect a waste liquid receiver 55.The electromagnetic wave coupling sensor is made up of sample cell 56, sensing membrane 57, prism 58 and locator card 59.Sample cell 56 two ends are fixed on the horizontal stand 510, are the film surfaces of sensing membrane 57 below the pond body of sample cell, use washer sealing each other.Be prism 58 below the sensing membrane 57, use the liquid adhesion between them.The following of prism 58 fixed with locator card 59.
Temperature control system mainly is made up of heat transfer medium, radiating tube, temperature sensor and temperature control circuit.Wherein heat transfer medium 512 flows through sample cell 56, controls the temperature of sample cell 56 by regulating temperature sensor 513 and temperature control circuit 514.
The basic structure of detection system E such as Fig. 6.Mainly form by reflected light Deviation rectifier, detecting device and angle demodulator.Angle demodulator can be a universal stage, can mechanical angle modulation, the also automatically controlled angle modulation of stepper motor.System construction is: the reflected light Deviation rectifier is formed a lens combination by lens 61 and lens 62, is associated in by fixed support 64 with colored CCD detecting device 63, by extension bar 65 they is fixed on screw on the axis of rotation of universal stage 66.The angle of regulating universal stage can make reflected light Deviation rectifier and detecting device rotate around the axle center together.
Data acquisition management system F: this part is mainly handled by image pick-up card, picture editting and data analysis software is formed, and is used to finish the collection and the tasks such as analyzing and processing and experimental result output of the monitoring of signal and storage, data.Wherein image pick-up card (can adopt the image pick-up card of OK-C30A image pick-up card or other model) carries out real-time collection, demonstration, storage and the output of image.The picture editting handles and data analysis software is carried out off-line analysis to image, choosing at random and the real-time optimization function of integral body, topography's collection can be provided, the signal comparison in the two dimensional image and the conversion of image and spectral line be can carry out, the function optimization regulation and control of image and the dynamic process of monitoring sample spot according to the variation of picture signal in real time realized.Also the color of image can be converted into absolute figure according to funtcional relationship, sample is carried out qualitative and quantitative analysis.Fig. 7 handles and the data analysis software process flow diagram for the picture editting of resonance imager of light-exciting color surface piasma of the present invention.
The instrument system principle of work is: on the sensing membrane and the interface of sample cell of the directional light that light source A sends through shining the C of sample analysis system after the reflection of incident angle of light modulating system B and the angular modulation.When behind sensing membrane generation surface plasma body resonant vibration, can cause catoptrical color to change.The light that goes out from prismatic reflection enters detection system E, detection system changes detected light signal into electric signal and is real-time transmitted to data acquisition management system F, data acquisition management system is monitored in real time to image, and data analysis is handled, and realizes qualitative and quantitative analysis.In testing process, can start the temperature of temperature control system D accuracy controlling sample cell.Fig. 8 is the program flow diagram of the experiment control system of multi-functional resonance imager of light-exciting color surface piasma.Fig. 9 is the software flow pattern of multi-functional resonance imager of light-exciting color surface piasma.
The detection example of sample
Embodiment one:
The prism bottom is coated with the golden film of one deck nanometer grade thickness as sensing membrane, make detecting device with colored CCD, measure the coloured surface plasma resonant imaging figure and the color response value of the ethanol water of different volumes percent concentration, see Table 1, table 1 is the colored resonance image-forming figure and the chroma-luminance value thereof of the ethanol water of the different volumes percent concentration measured of multi-functional resonance imager of light-exciting color surface piasma.
Embodiment two:
The prism bottom is coated with the gold of one deck nanometer grade thickness as sensing membrane, make detecting device with colored CCD, record the concentration of volume percent of actual liquor sample and the comparison of demarcating concentration value, see Table 2, table 2 is the comparison of concentration value with the sample demarcation concentration value of the actual liquor sample measured of multi-functional resonance imager of light-exciting color surface piasma.
Embodiment three:
The gold that the prism bottom is coated with one deck nanometer grade thickness is as sensing membrane, and sensing membrane adopts colored CCD to make detecting device by immunoglobulin G microarray in the chemical modification self assembly, records coloured surface plasma resonant imaging figure in air, sees Figure 10.Figure 12 is its linear spectral line chart that is converted into by its making software.
Embodiment four:
The method identical with embodiment three usefulness modified sensing membrane, modifies insulin.Instrument adopts CCD to make detecting device, measures color table surface plasma resonance image in the aqueous solution, sees Figure 11.
Embodiment five:
Be fixed into shooting angle and adopt CCD to make detecting device and connect grating, adopt identical method to modify sensing membrane, covalent bonding goat anti-human immunoglobulin G on the sensing membrane with embodiment three.At first in sample cell, feed the buffer solution of immunoglobulin G while, reacted 30 minutes, start real-time detecting operation simultaneously, the variation of monitoring solution resonant wavelength, and then the buffer solution that injects the anti-immunoglobulin G while of rabbit in sample cell reacted 30 minutes, cause resonant wavelength to be subjected to displacement behind the antigen antibody interaction and see Figure 13, realize thus the interactional real-time and performance analysis of antigen-antibody.
Table 1
Table 2
Claims (10)
1, a kind of resonance imager of light-exciting color surface piasma mainly is made up of light source, incident angle of light modulating system, sample analysis system, temperature control system, detection system, data acquisition management system; Wherein:
One sample analytic system, one side set gradually a light source and an incident angle of light modulating system; Relative opposite side sets gradually a detection system and a data acquisition management system;
Described detection system comprises a reflected light Deviation rectifier and a reflected light detector; This reflected light detector is connected with described data acquisition management system;
Near the sample cell of sample analysis system, connect temperature control system is set;
The sample cell both sides are provided with the sample feeding pipeline and go out the sample pipeline in the sample analysis system, and a micro pump communicates by sample introduction pipeline and sample cell and controls the flow velocity and the flow of sample introduction; The sample cell two ends are fixed on the horizontal stand, the pond body down, with the film of sensing membrane surface washer sealing, a sensing membrane bottom surface and a prism back side adhesion; The surface of sensing membrane has the high electricity of free electron layer and leads material;
The light that light source sends enters the sample analysis system after the modulation of incident angle of light modulating system, excite the sensing membrane surface plasma to produce resonance by prism, directly causes catoptrical color to change; The reflected light that penetrates from prism enters detection system, is detected by reflected light detector behind the reflected light Deviation rectifier, and is transferred to data acquisition management system by electric signal and carries out data processing, realizes qualitative and quantitative analysis;
Described light source is the continuous wave incident light.
2, resonance imager of light-exciting color surface piasma as claimed in claim 1, wherein the continuous wave incident light is injected the sample analysis system through an incident angle of light regulating system, and this incident light regulating system is that an optical mirror is fixed on the mirror support of an adjustable angle.
3, resonance imager of light-exciting color surface piasma as claimed in claim 1 or 2, wherein the continuous wave incident light is white light or the polychromatic light that halogen tungsten lamp, xenon lamp, Nernst glower, Elema lamp or light emitting diode send, and modulates through an optical beam expander or beam compressor; For nonpolarized light, before optical beam expander or beam compressor, add the p-type polarized light polarizer.
4, resonance imager of light-exciting color surface piasma as claimed in claim 1, wherein prism is that inorganic optical glass, chemosynthesis optical resin plastics or tool light transmission fluent material are made.
5, resonance imager of light-exciting color surface piasma as claimed in claim 1, wherein reflected light detector is colored charge-coupled device, colored complementary MOS image sensor, charge-injection detector(CID), photomultiplier or camera, as the lens of reflected light Deviation rectifier and its with optical axis be fixed on the runing rest, form detection system, reflected light is proofreaied and correct and focused.
6, resonance imager of light-exciting color surface piasma as claimed in claim 1, wherein the body top, pond of the sample cell in the sample analysis system is connected with the heat dissipation pipeline of a temperature control system, this heat dissipation pipeline connects a cooling liquid container, and this cooling liquid container connects the temperature control circuit of a temperature control regulator.
7, resonance imager of light-exciting color surface piasma as claimed in claim 1, wherein sensing membrane is golden film, silverskin or aluminium film, thickness is 10-200nm.
8, resonance imager of light-exciting color surface piasma as claimed in claim 1, wherein data acquisition management system carries out the analyzing and processing and the experimental result output of real-time collection, demonstration, storage and the data of image.
9, resonance imager of light-exciting color surface piasma as claimed in claim 1, wherein any position configuration monochromator or utilize the monochromatic light electric transducer before the reflected light detector promptly is convertible into black and white surface plasma resonance image-forming instrument.
10, resonance imager of light-exciting color surface piasma as claimed in claim 1, wherein any position configuration dispersion system before the reflected light detector can change wavelength into and ask the surface plasma resonance optical spectrum instrument of surveying.
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| EP2245467B1 (en) * | 2008-02-05 | 2022-04-06 | Pocared Diagnostics Ltd. | System for conducting the identification of bacteria in biological samples |
| CN102998097B (en) * | 2011-09-19 | 2015-06-03 | 中国科学院电子学研究所 | Attenuated total reflection optical measurement platform |
| CN102721668A (en) * | 2012-06-15 | 2012-10-10 | 天津大学 | On-line refractive index measurement device and method based on wavelength modulation type surface plasmon resonance (SPR) technology |
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| BR112020017726A2 (en) * | 2018-03-02 | 2020-12-22 | Genzyme Corporation | MULTIVARIATE SPECTRAL ANALYSIS AND BIOMANUFACTURING MONITORING |
| CN115598060A (en) * | 2022-09-13 | 2023-01-13 | 厦门大学（Cn） | High-flux automatic detection device and method for single-particle scattering spectrum |
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| CN1203382A (en) * | 1998-07-01 | 1998-12-30 | 吉林大学 | All wavelength surface plasma excimer resonance photochemical sensor |
| CN1717581A (en) * | 2002-10-28 | 2006-01-04 | 华盛顿大学 | Wavelength tunable surface plasmon resonance sensor |
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| US20050134860A1 (en) * | 2003-10-24 | 2005-06-23 | Chinowsky Timothy M. | Surface plasmon resonance imaging system and method |
| CN1629618A (en) * | 2003-12-16 | 2005-06-22 | 中国科学院化学研究所 | Multifunctional photoexcited surface plasma resonant imager |
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