CN109970587A - A kind of L-aspartic acid separation and purification device and method for separation and purification of L-aspartic acid - Google Patents

Abstract

The invention discloses an L-aspartic acid separation and purification device, comprising an enzyme liquid storage tank, the enzyme liquid storage tank is connected with a column chromatography separation device, the column chromatography separation device is connected with a membrane filter device, and the filter membrane filter device is connected with There is a concentration and crystallization device; the column chromatography separation device includes a liquid tray, and the top of the liquid tray is connected with several chromatography columns. The above-mentioned device disclosed in the present invention solves the technical problems of low yield, high cost and large pollution of using an extraction device to separate and purify L-aspartic acid in the prior art. At the same time, the present invention provides a method for separating and purifying L-aspartic acid, which specifically includes four steps of column chromatography adsorption, elution, microfiltration membrane and nanofiltration membrane filtration, and concentration and crystallization. The technical solution disclosed by the invention not only has high separation efficiency and high yield, but also has a relatively high purity of L-aspartic acid obtained by separation, and at the same time, adopts a column chromatography separation method to separate the amount of sewage and waste gas, and reduces industrial production pollution. Reduced environmental disposal costs.

Description

A kind of L-aspartic acid separation and purification device and its separation and purification of L-aspartic acid method

technical field

The invention relates to an amino acid extraction column chromatography separation device, in particular to an L-aspartic acid separation and purification device and a method for separating and purifying L-aspartic acid.

Background technique

L-Aspartic acid, whose structure is shown below:

L-aspartic acid can not only provide amino acid source for the human body, but also can be used as an antidote for medicinal purposes such as ammonia. Regarding the industrialized preparation method of L-aspartic acid, the prior art is mostly prepared by means of chemical synthesis, but the defect of chemical synthesis is that the pollution is large.

In order to overcome the technical defects of chemical synthesis of L-aspartic acid, the researchers used the method of biological fermentation to prepare the fermentation liquid of L-aspartic acid precursor, and then enzymatically hydrolyzed the fermentation liquid to obtain L-aspartic acid rich in fermentation liquid. Acid-controlled enzymatic conversion solution. However, in the process of obtaining L-aspartic acid by biological fermentation, it has always been a difficult technical problem to separate and control the L-aspartic acid in the enzymatic transformation liquid.

In the prior art, in order to separate L-aspartic acid from the control enzyme conversion liquid, extraction means are mostly used to obtain L-aspartic acid through extraction.

However, for the above-mentioned extraction method, since the control enzyme conversion solution contains various other impurities besides L-aspartic acid, L-aspartic acid can be obtained by repeated extraction and repeated crystallization. The yield of L-aspartic acid obtained after extraction and crystallization is abnormally low.

For the difficulty of separation and purification by extraction means or the low separation yield of extraction means, column chromatography is currently used to solve the problem.

Column chromatographic separation technology, that is, chromatographic separation through chromatographic columns, has been applied from small chromatographic columns used in laboratories to large chromatographic columns produced by industrial separation. The separation liquid is added to a large-scale chromatography column, and the adsorbent in the chromatography column, such as silica gel, etc., separates the substances in the separation liquid by chromatography. Because the industrially produced large-scale chromatography column has a certain height and width, the number of theoretical plates is large, the separation efficiency is high, and at the same time, the amount of one-time separation is also large.

However, up to now there is no column chromatography equipment and applicable chromatography method that can be applied to L-aspartic acid. In the prior art, the traditional multiple extraction-crystallization technology is still used for separation, and the amount of waste liquid produced by the extraction method is relatively large, resulting in high industrialized sewage treatment cost and high extraction cost.

SUMMARY OF THE INVENTION

The technical problem to be solved by the present invention is to provide a L-aspartic acid separation and purification device and a method for separation and purification of L-aspartic acid.

The present invention solves the above-mentioned technical problems through the following technical solutions:

An L-aspartic acid separation and purification device, comprising an enzyme liquid storage tank, the enzyme liquid storage tank is connected with a column chromatography separation device, and the column chromatography separation device is connected with a filter membrane filtration device, the filter membrane The filtering device is communicated with a concentration and crystallization device;

The column chromatography separation device includes a liquid pan, the top of the liquid pan is connected with a plurality of chromatography columns, a first pipeline is connected between the chromatography columns, and the first pipeline is connected with a second pipeline, so the The second pipeline is communicated with the enzyme liquid storage tank;

The bottom of the liquid pan is connected with a third pipeline, the third pipeline is connected with a membrane filtration device, and the membrane filtration device is connected with a concentration and crystallization device;

The membrane filtration device includes a microfiltration membrane filtration device and a nanofiltration membrane filtration device, and the top of the nanofiltration membrane filtration device and the top of the microfiltration membrane filtration device are communicated through a fifth pipeline;

The bottom of the microfiltration membrane filtration device is communicated with the third pipeline, and the bottom of the nanofiltration membrane filtration device is communicated with the concentration and crystallization device through the fourth pipeline;

The first pipeline, the second pipeline, the third pipeline, the fourth pipeline, and the fifth pipeline are all provided with valves.

Preferably, six chromatography columns are symmetrically distributed on the top of the liquid pan, and the chromatography columns are symmetrically distributed on the side of the bottom of the liquid pan with the center of the liquid pan as the center of symmetry;

The chromatographic columns located at the diagonal positions are communicated with each other through first pipes, the first pipes are in penetrating communication with each other, and the penetrating parts of the first pipes are vertically connected to the second pipes upward.

Preferably, the ends of the two ends of the first pipe respectively penetrate through the top of the corresponding chromatography column, and the ends of the two ends of the first pipe are respectively connected with metal filter balls.

Preferably, the chromatography column comprises a top cover, a bottom cover and a column body, the top cover is connected to the top of the column body, the bottom cover is connected to the bottom of the column body, the top cover is provided with an inner cavity, and the metal filter The ball is located in the inner cavity of the top cover, the column is provided with a chromatography cavity, the chromatography cavity is communicated with the inner cavity of the top cover, and an anti-impact disk is connected to the top of the chromatography cavity;

A plurality of through holes are opened on the anti-shock disk, the through holes are connected to the chromatography cavity, and a filter screen is fixedly connected to the through holes;

The bottom cover is provided with an inner cavity, the inner cavity of the bottom cover communicates with the chromatography cavity, and the bottom cover is communicated with the top of the liquid pan through a liquid drain pipe, and the liquid drain pipe is provided with a valve.

Preferably, the chromatography cavity is filled with ion exchange resin.

Preferably, the concentrated crystallization device comprises a concentrated crystallization kettle, a stirring device is arranged in the concentrated crystallization kettle, and a vacuum device is communicated with the concentrated crystallization kettle.

Preferably, the inside of the liquid pan is hollow, and a glass observation area is provided on the side wall of the liquid pan.

Preferably, scale markings are provided on the glass observation area.

Preferably, a layer of circular filter screen is fixedly connected to the bottom of the liquid pan, and the circular filter screen covers the bottom surface of the liquid pan.

The present invention also discloses a method for separating and purifying L-aspartic acid by adopting the above-mentioned L-aspartic acid separation and purification device, comprising the following steps:

S1, pump 50L, L-aspartic acid concentration content of 160-210g/L regulating enzyme transformation liquid into the enzyme liquid storage tank, after adjusting the pH of regulating enzyme transformation liquid to 4-8, open the valve, The regulating enzyme conversion solution is added to the column chromatography separation device for adsorption, and L-aspartic acid is adsorbed in the column chromatography separation device, and the flow rate of the regulating enzyme conversion solution flowing into the column chromatography separation device is controlled to be 100-2000 mL/min;

S2. After the adsorption, the sodium hydroxide solution is pumped into the enzyme liquid storage tank, as the eluent, the valve is opened, and the L-aspartic acid adsorbed in the column chromatography separation device is eluted with sodium hydroxide to control the hydrogen The elution flow rate of sodium oxide is 100-2000mL/min;

S3. During the elution process, open the valve, and the eluent enters the microfiltration membrane filtration device and the nanofiltration membrane filtration device in turn, and then enters the concentrated crystallization device;

S4, after concentrating the eluent under reduced pressure, adding sulfuric acid to adjust the pH value of the concentrated solution to 1.5-3.0, adding L-aspartic acid seed crystals to crystallize, and after filtering and drying to obtain L-aspartic acid Pure acid.

Compared with the prior art, the present invention has the following advantages:

The device disclosed by the invention solves the technical problems of low yield, high cost and large pollution in the prior art by extracting and purifying L-aspartic acid. The device disclosed in the present invention provides a device suitable for industrial column chromatography separation of L-aspartic acid. Using the above device to separate L-aspartic acid by column chromatography not only has high separation efficiency and high yield, but also obtains L-aspartic acid. -The purity of aspartic acid is also relatively high. At the same time, the separation method is adopted by column chromatography, and the amount of sewage and waste gas is small, which reduces industrial pollution and reduces the cost of environmental protection treatment.

The separation and purification method disclosed by the invention replaces the extraction method used in traditional industrialization, and has the advantages of high yield, 99.9% purity and simple method.

Description of drawings

Fig. 1 is the overall structure schematic diagram of the embodiment of the present invention;

Fig. 2 is the structural representation of the concentrated crystallization device and the membrane filtration device in the embodiment of the present invention;

Fig. 3 is the structural representation of the column chromatography separation device in the embodiment of the present invention;

4 is a cross-sectional view of a chromatography column in an embodiment of the present invention;

Fig. 5 is the partial enlarged structural schematic diagram of the part A in Fig. 4 in the embodiment of the present invention;

6 is a schematic structural diagram of an anti-shock disc in an embodiment of the present invention;

Fig. 7 is the connection relation diagram of the first pipeline and the second pipeline in the embodiment of the present invention;

Fig. 8 is the infrared detection spectrum of pure L-aspartic acid obtained by crystallization in Example 2 of the present invention.

Detailed ways

The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and provides a detailed implementation manner and a specific operation process, but the protection scope of the present invention is not limited to the following implementation. example.

Example 1L-Aspartic acid separation and purification device

As shown in Figure 1--7, a kind of L-aspartic acid separation and purification device, comprises enzyme liquid storage tank 1, and enzyme liquid storage tank 1 is communicated with column chromatography separation device 2 (column chromatography separation device 2 is located in the enzyme liquid storage tank 1). The bottom of the liquid storage tank 1), the column chromatography separation device 2 is communicated with a membrane filter device 3 (the membrane filter device 3 is located below the column chromatography separation device 2), and the membrane filter device 3 is communicated with a concentrated crystallization device 4 ( The concentration crystallization device 4 is located below the membrane filtration device 3).

In the above-mentioned device: the membrane filtration device 3 includes a microfiltration membrane filtration device 31 and a nanofiltration membrane filtration device 32 (the microfiltration membrane filtration device 31 and the nanofiltration membrane filtration device 32 communicate with each other), wherein, the microfiltration membrane filtration device 31 and The nanofiltration membrane filtration device 32 is the conventional microfiltration membrane filtration equipment and nanofiltration membrane filtration equipment disclosed in the prior art, and its main structure includes the corresponding microfiltration membrane and nanofiltration membrane. The specific structure and working principle of the microfiltration membrane filtration device 31 and the nanofiltration membrane filtration device 32 disclosed in the present invention can be known.

Meanwhile, the concentrated crystallization device 4 is a conventional concentrated crystallization kettle disclosed in the prior art, and its main structure includes a kettle body, a stirring device arranged on the kettle body, and a vacuum device connected to the top of the kettle body, such as a vacuum pump, which utilizes the vacuum device to The eluate in the kettle is concentrated.

The improvement point of the present invention is:

A column chromatography separation device 2 is provided, specifically, the column chromatography separation device 2 includes a liquid pan 22 (the interior of the liquid pan 22 is hollow, and a glass observation area is provided on the side wall of the liquid pan 22, and the glass observation area is set with tempered glass, The glass observation area is provided with a scale mark 221, and the scale mark 221 is used to mark the liquid level in the liquid pan 22), and the top of the liquid pan 22 is symmetrically distributed with 6 chromatography columns 21 (the length of each chromatography column 21 is 6.5m, the radius is 1m), the chromatography column 21 takes the center of the liquid pan 22 as the center of symmetry, and is symmetrically distributed on the side of the top of the liquid pan 22 . The bottom of the chromatography column 21 is connected to the liquid pan 22 (the bottom of the chromatography column 21 is connected to a drain pipe, and the drain pipe is connected to the liquid pan 22).

The two chromatographic columns 21 located at the diagonal positions (the two chromatographic columns 21 are located at two ends of a diameter line respectively) are communicated through the first pipeline 211 (the tops of the chromatographic columns 21 are connected through the first pipeline 211 connection), the first pipes 211 are cross-connected, and the intersection of the first pipes 211 is vertically connected to the second pipe 212 upward. (At the same time, each first pipe 211 is provided with two valves, and the valves are respectively located at both ends of the first pipe 211 near the position where the top cover 201 of the chromatography column 21 is located).

The structure of the chromatography column 21 is specifically:

The chromatography column 21 includes a top cover 201, a bottom cover 202, and a column. The top cover 201 is connected to the top of the column, the bottom cover 202 is connected to the bottom of the column, and the top cover 201 is provided with an inner cavity. The end penetrates into the inner cavity of the top cover 201, and the end is connected to a metal filter ball 2111 (the metal filter ball 2111 is woven from a metal filter screen, and the end of the first pipe 211 extends into the metal filter ball 2111). , The purpose of the metal filter ball 2111 is to prevent insoluble matter from entering the chromatographic cavity and affect the chromatographic effect).

The column is provided with a chromatography cavity (the chromatography cavity is filled with ion exchange resin, and the ion exchange resin is 732 type cation exchange resin), the chromatography cavity is connected to the inner cavity of the top cover 201, and the top position of the chromatography cavity The anti-shock plate 213 is connected (a plurality of through holes 2131 are opened on the anti-shock plate 213, the through holes 2131 are connected to the chromatography cavity, and the filter screen is fixedly connected to the through holes 2131. The purpose of using the anti-shock plate 213 is to prevent the Immediately after the eluent is added to the chromatographic cavity, the packed ion exchange resin powder is impacted and deformed). First fill the ion exchange resin in the chromatography cavity, and then screw the anti-shock disk 213 to the position of the cavity mouth of the chromatography cavity (an external thread portion 2132 is provided on the side wall of the anti-shock disk 213, and the corresponding chromatographic cavity The inner wall of the cavity of the cavity is provided with corresponding internal threads). According to the existing method, the top cover 201 and the cylinder are designed to be connected in a detachable manner, for example, the top cover 201 is screwed to the top of the cylinder.

Similarly, the bottom cover 202 is provided with an inner cavity, the inner cavity of the bottom cover 202 is connected to the chromatography cavity, the bottom cover 202 is connected to the top of the liquid pan 22 through a drain pipe, and the drain pipe is provided with a valve.

Since the two chromatographic columns 21 located at the diagonal positions are communicated through the first pipelines 211, the six chromatographic columns 21 have three first pipelines 211, and the three first pipelines 211 are located on the same horizontal plane, There is a cross between the three first pipes 211, and the cross position is where the three first pipes 211 communicate with each other, and the second pipe 212 is vertically connected upward at the intersection, and the second pipe 212 is connected to the bottom of the enzyme liquid storage tank 1. .

The bottom of the liquid pan 22 (the bottom of the liquid pan 22 is fixedly connected with a layer of circular filter screen, the circular filter screen covers the bottom surface of the liquid pan 22, the function of the circular filter screen is to filter and prevent clogging of the microfiltration membrane filter device 31 and nanofiltration. The bottom center of the membrane filter device 32 (circular filter screen is not shown in the figure) is communicated with a third pipeline 312, the third pipeline 312 is connected to the bottom of the microfiltration membrane filter device 31, and the top of the microfiltration membrane filter device 31 passes through The fifth pipeline 311 communicates with the top of the nanofiltration membrane filtration device 32 , and the bottom of the nanofiltration membrane filtration device 32 communicates with the concentration and crystallization device 4 through the fourth pipeline 321 .

The first pipe 211 , the second pipe 212 , the third pipe 312 , the fourth pipe 321 , and the fifth pipe 311 are all provided with valves.

Embodiment 2 The method for separating and purifying L-aspartic acid

As shown in Figure 1-7, the method for separating and purifying L-aspartic acid includes the following steps:

S1, with 50L, the L-aspartic acid concentration content is that the regulating enzyme conversion liquid of 160g/L is pumped into the enzyme liquid storage tank 1, after the pH of the regulating enzyme conversion liquid is adjusted to 4, open the second pipeline 212 The valve is adjusted to enter the first pipeline 211 through the second pipeline 212, and the number of valves on the first pipeline 211 is selected to be opened according to the actual adjustment of the liquid volume of the enzymatic transformation solution, that is, the number of valves that pass through the chromatography column 21 is selected. number. Control and regulate the flow rate that the enzyme conversion solution flows into the chromatographic column 21 to be 100mL/min;

S2, the adsorption is completed, the sodium hydroxide solution is pumped into the enzyme liquid storage tank 1, as the eluent, open the valve on the second pipeline 212, and the valve on the drain pipe at the bottom of the chromatography column 21, sodium hydroxide The solution is separated from the chromatography column 21 with L-aspartic acid, and the L-aspartic acid eluate enters the liquid pan 22, and the elution flow rate of the controlled sodium hydroxide is 100 mL/min;

S3. During the elution process, open the valve on the third pipeline 312, and the L-aspartic acid eluate enters the microfiltration membrane filtration device 31 from the third pipeline 312, and after being filtered by the microfiltration membrane filtration device 31, Enter the nanofiltration membrane filtration device 32 from the fifth pipeline 311, and then enter the concentration and crystallization device 4 from the fourth pipeline 321 for decompression concentration;

S4. After concentrating the eluent under reduced pressure, adding sulfuric acid to adjust the pH value of the concentrated solution to 1.5, adding L-aspartic acid seed crystals for crystallization, filtering and drying to obtain pure L-aspartic acid Taste. The purity detected by HPLC was 99.9%, and the yield was 91.6%.

The infrared detection spectrum of pure L-aspartic acid is shown in Figure 8.

Embodiment 3 The method for separating and purifying L-aspartic acid

As shown in Figure 1--7, the method for separating and purifying L-aspartic acid includes the following steps:

S1, with 50L, the L-aspartic acid concentration content is that the regulating enzyme conversion liquid of 210g/L is pumped into the enzyme liquid storage tank 1, after the pH of the regulating enzyme conversion liquid is adjusted to 8, open the second pipeline 212 The valve is adjusted to enter the first pipeline 211 through the second pipeline 212, and the number of valves on the first pipeline 211 is selected to be opened according to the actual adjustment of the liquid volume of the enzymatic transformation solution, that is, the number of valves that pass through the chromatography column 21 is selected. number. Control and regulate the flow rate that the enzyme conversion solution flows into the chromatography column 21 to be 2000mL/min;

S2, the adsorption is completed, the sodium hydroxide solution is pumped into the enzyme liquid storage tank 1, as the eluent, open the valve on the second pipeline 212 and the valve on the drain pipe at the bottom of the chromatography column 21, sodium hydroxide The solution is separated from the chromatography column 21 with L-aspartic acid, and the L-aspartic acid eluate enters the liquid pan 22, and the elution flow rate of the controlled sodium hydroxide is 500 mL/min;

S3. During the elution process, open the valve on the third pipeline 312, and the L-aspartic acid eluate enters the microfiltration membrane filtration device 31 from the third pipeline 312, and after being filtered by the microfiltration membrane filtration device 31, Enter the nanofiltration membrane filtration device 32 from the fifth pipeline 311, and then enter the concentration and crystallization device 4 from the fourth pipeline 321 for decompression concentration;

S4. After concentrating the eluent under reduced pressure, adding sulfuric acid to adjust the pH value of the concentrated solution to 1.5, adding L-aspartic acid seed crystals for crystallization, filtering and drying to obtain pure L-aspartic acid Taste. The purity detected by HPLC was 99.9%, and the yield was 90.5%.

Embodiment 4 The method for separating and purifying L-aspartic acid

As shown in Figure 1--7, the method for separating and purifying L-aspartic acid includes the following steps:

S1, with 50L, the L-aspartic acid concentration content is that the regulating enzyme transformation liquid of 180g/L is pumped into the enzyme liquid storage tank 1, after the pH of the regulation regulating enzyme transformation liquid is to 8, open the second pipeline 212 The valve is adjusted to enter the first pipeline 211 through the second pipeline 212, and the number of valves on the first pipeline 211 is selected to be opened according to the actual adjustment of the liquid volume of the enzymatic transformation solution, that is, the number of valves that pass through the chromatography column 21 is selected. number. The flow rate that the control and regulation enzyme conversion liquid flows into the chromatography column 21 is 500mL/min;

S2, the adsorption is completed, the sodium hydroxide solution is pumped into the enzyme liquid storage tank 1, as the eluent, open the valve on the second pipeline 212 and the valve on the drain pipe at the bottom of the chromatography column 21, sodium hydroxide The solution is separated from the chromatography column 21 with L-aspartic acid, and the L-aspartic acid eluate enters the liquid pan 22, and the elution flow rate of the controlled sodium hydroxide is 100 mL/min;

S3. During the elution process, open the valve on the third pipeline 312, and the L-aspartic acid eluate enters the microfiltration membrane filtration device 31 from the third pipeline 312, and after being filtered by the microfiltration membrane filtration device 31, Enter the nanofiltration membrane filtration device 32 from the fifth pipeline 311, and then enter the concentration and crystallization device 4 from the fourth pipeline 321 for decompression concentration;

S4, after concentrating the eluent under reduced pressure, adding sulfuric acid to adjust the pH value of the concentrated solution to 2.8, adding L-aspartic acid seed crystals, carrying out crystallization, filtering and drying to obtain pure L-aspartic acid Taste. The purity detected by HPLC was 99.9%, and the yield was 90.3%.

Embodiment 5 The method for separating and purifying L-aspartic acid

As shown in Figure 1--7, the method for separating and purifying L-aspartic acid includes the following steps:

S1, with 50L, the L-aspartic acid concentration content is that the regulating enzyme conversion liquid of 200g/L is pumped into the enzyme liquid storage tank 1, after adjusting the pH of the regulating enzyme conversion liquid to 6, open the second pipeline 212 The valve is adjusted to enter the first pipeline 211 through the second pipeline 212, and the number of valves on the first pipeline 211 is selected to be opened according to the actual adjustment of the liquid volume of the enzymatic transformation solution, that is, the number of valves that pass through the chromatography column 21 is selected. number. Control and regulate the flow rate that the enzyme conversion solution flows into the chromatographic column 21 to be 100mL/min;

S2, the adsorption is completed, the sodium hydroxide solution is pumped into the enzyme liquid storage tank 1, as the eluent, open the valve on the second pipeline 212 and the valve on the drain pipe at the bottom of the chromatography column 21, sodium hydroxide The solution is separated from the chromatography column 21 with L-aspartic acid, and the L-aspartic acid eluate enters the liquid pan 22, and the elution flow rate of the controlled sodium hydroxide is 500 mL/min;

S3. During the elution process, open the valve on the third pipeline 312, and the L-aspartic acid eluate enters the microfiltration membrane filtration device 31 from the third pipeline 312, and after being filtered by the microfiltration membrane filtration device 31, Enter the nanofiltration membrane filtration device 32 from the fifth pipeline 311, and then enter the concentration and crystallization device 4 from the fourth pipeline 321 for decompression concentration;

S4. After concentrating the eluent under reduced pressure, adding sulfuric acid to adjust the pH value of the concentrated solution to 3.0, adding L-aspartic acid seed crystals for crystallization, filtering and drying to obtain pure L-aspartic acid Taste. The purity detected by HPLC was 99.9%, and the yield was 92.7%.

The above descriptions are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the protection of the present invention. within the range.

Claims (10)

1. a L-aspartic acid separation and purification device, is characterized in that, comprises enzyme liquid storage tank, described enzyme liquid storage tank is communicated with column chromatography separation device, and described column chromatography separation device is communicated with membrane filtration device, the membrane filtration device is communicated with a concentration and crystallization device; The column chromatography separation device includes a liquid pan, the top of the liquid pan is connected with a plurality of chromatography columns, a first pipeline is connected between the chromatography columns, and the first pipeline is connected with a second pipeline, so the The second pipeline is communicated with the enzyme liquid storage tank; The bottom of the liquid pan is connected with a third pipeline, the third pipeline is connected with a membrane filtration device, and the membrane filtration device is connected with a concentration and crystallization device; The membrane filtration device includes a microfiltration membrane filtration device and a nanofiltration membrane filtration device, and the top of the nanofiltration membrane filtration device and the top of the microfiltration membrane filtration device are communicated through a fifth pipeline; The bottom of the microfiltration membrane filtration device is communicated with the third pipeline, and the bottom of the nanofiltration membrane filtration device is communicated with the concentration and crystallization device through the fourth pipeline; The first pipeline, the second pipeline, the third pipeline, the fourth pipeline, and the fifth pipeline are all provided with valves. 2. L-aspartic acid separation and purification device according to claim 1, is characterized in that, the top of described liquid pan is symmetrically distributed with 6 chromatographic columns, and described chromatographic column takes the disk center of liquid pan as Symmetrical center, symmetrically distributed on the side of the bottom of the liquid pan; The chromatographic columns located at the diagonal positions are communicated with each other through first pipes, the first pipes are in penetrating communication with each other, and the penetrating parts of the first pipes are vertically connected to the second pipes upward. 3. L-aspartic acid separation and purification device according to claim 1, is characterized in that, the end of described first pipeline two ends penetrates the top of corresponding chromatography column respectively, and the end of described first pipeline is two The ends of the ends are respectively connected with metal filter balls. 4. L-aspartic acid separation and purification device according to claim 3, is characterized in that, described chromatographic column comprises top cover, bottom cover and cylinder, described top cover connects the top of cylinder, bottom cover The bottom of the column is connected, the top cover is provided with an inner cavity, the metal filter ball is located in the inner cavity of the top cover, the column is provided with a chromatography cavity, and the chromatography cavity is connected to the top cover. an inner cavity, an anti-shock disk is connected to the cavity top position of the chromatography cavity; A plurality of through holes are opened on the anti-shock disk, the through holes are connected to the chromatography cavity, and a filter screen is fixedly connected to the through holes; The bottom cover is provided with an inner cavity, the inner cavity of the bottom cover communicates with the chromatography cavity, and the bottom cover is communicated with the top of the liquid pan through a liquid drain pipe, and the liquid drain pipe is provided with a valve. 5 . The L-aspartic acid separation and purification device according to claim 4 , wherein the chromatographic cavity is filled with ion exchange resin. 6 . 6. L-aspartic acid separation and purification device according to claim 1, is characterized in that, described concentrated crystallization device comprises concentrated crystallization still, is provided with stirring device in described concentrated crystallization still, and described concentrated crystallization still communicates with There is a vacuum device. 7 . The L-aspartic acid separation and purification device according to claim 1 , wherein the inside of the liquid pan is hollow, and a glass observation area is provided on the side wall of the liquid pan. 8 . 8 . The L-aspartic acid separation and purification device according to claim 7 , wherein a scale mark is provided on the glass observation area. 9 . 9. L-aspartic acid separation and purification device according to claim 8, is characterized in that, the bottom in the described liquid pan is fixedly connected with a layer of circular filter screen, and the described circular filter screen covers the underside. 10. a method for adopting L-aspartic acid separation and purification device as described in any one of claims 1-9 to separate and purify L-aspartic acid, is characterized in that, comprises the following steps: S1, pump 50 L, the L-aspartic acid concentration content is 160-210g/L regulating enzyme transformation liquid into the enzyme liquid storage tank, after adjusting the pH of regulating enzyme transformation liquid to 4-8, open the valve, Add the regulating enzyme conversion solution to the column chromatography separation device for adsorption, L-aspartic acid is adsorbed in the column chromatography separation device, and control the flow rate of the regulating enzyme conversion solution flowing into the column chromatography separation device to be 100-2000mL/min ; S2. After the adsorption is completed, the sodium hydroxide solution is pumped into the enzyme liquid storage tank, and as the eluent, the valve is opened, and the L-aspartic acid adsorbed in the column chromatography separation device is eluted with sodium hydroxide to control the hydrogen The elution flow rate of sodium oxide is 100-2000mL/min; S3. During the elution process, open the valve, and the eluent enters the microfiltration membrane filtration device and the nanofiltration membrane filtration device in turn, and then enters the concentrated crystallization device; S4, after concentrating the eluent under reduced pressure, adding sulfuric acid to adjust the pH value of the concentrated solution to 1.5-3.0, adding L-aspartic acid seed crystals to crystallize, and after filtering and drying to obtain L-aspartic acid Pure acid.