CN109896713A - A method of sewage is handled using ABS microbial bacterial agent - Google Patents
A method of sewage is handled using ABS microbial bacterial agent Download PDFInfo
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Abstract
The present invention relates to a kind of methods using ABS microbial bacterial agent processing sewage, belong to technical field of sewage.To solve the problems, such as that existing sewage water treatment method can not complicated pollutant component in comprehensively solve sewage, the present invention provides a kind of method using ABS microbial bacterial agent processing sewage, sewage successively passes through septic tank or collecting pit, grid, conditioning tank, biological tank and sludge-water separating system；ABS microbial bacterial agent has been added in septic tank and biological tank, and primary degradation and degradable successively is carried out to pollutants such as COD, BOD, SS, Escherichia coli and TN.Microbial bacterial agent provided by the invention is aerobic can to react with slot with anaerobism, realize polynary fermentation and respiration, and treatment effect is had outstanding performance in the high environment of BOD, COD, colibacillus of excrement, pathogenic bacteria, virus, ammonia-nitrogen content.Apply the present invention to sewage treatment, the complicated pollutant component in the sewage that more can efficiently degrade comprehensively.
Description
Technical field
The invention belongs to technical field of sewage more particularly to a kind of sides using ABS microbial bacterial agent processing sewage
Method.
Background technique
Water is essential valuable source in one kind non-renewable resource and social sustainable development, urban life
Sewage, medical sewage and trade effluent are the main reason for causing water pollution, in sanitary sewage, medical sewage and trade effluent,
Containing a large amount of poisonous and hazardous microorganisms and chemical substance, the whole world is more than that 80% waste water is not obtained and is effectively treated with regard to direct progress
Discharge, significantly polluted water region and ecological environment, lead to the serious destruction of mankind's water safety and water ecological environment, threaten people
Health and normal life therefore how by sewage treatment means, remove the harmful substance in sewage, change give up into
Benefit is the common issue urgently to be resolved that current social faces.
AO technique, that is, anoxic_oxic process-Anoxic Oxic is a kind of follow-on sewage using activated sludge process
Treatment process not only can also have certain dephosphorization and denitrification effect with degradation of organic substances.AO technique is by anoxic and aerobic biochemical work
Skill is together in series, and anoxic section relies primarily on heterotroph and larger molecular organics, suspended matter, the dissolved organic matter in sewage are degraded
At small organic molecule；Aerobic section relies primarily on nitrifier and passes through nitrification for ammoxidation into nitrate nitrogen, nitrite nitrogen.Most
Afterwards, aerobic section muddy water mixed solution is back to anoxic section, it is complete by nitrate nitrogen denitrification at nitrogen under the action of denitrifying bacterium
The degradation of pairs of N element.
AAO technique, that is, anaerobic-anoxic-aerobic process-Anaerobic-Anoxic-Oxic is at a kind of common sewage
Science and engineering skill, by anaerobism, anoxic and it is aerobic be together in series, can be used for secondary sewage processing or three-level sewage treatment and middle water return
With.
But activated sludge used by AO, AAO technique is all the biologic treating technique in the first and second generation, utilizes sewage or dirt
Spontaneous bacterial in mud carries out nitrification and denitrification effect for organic pollutant degradation, make water body restore nitrogen cycle from net energy
Power has not dealt with modernization high concentration and high complicated sewage since strain is not complete or lazy weight.Microbial bacteria combines
The various means such as aerobic, anoxic, anaerobism and facility handle specific sewage, since adaptive capacity to environment and formula be not complete, can not
The sewage of complicated pollutant component and stubborn and stupid property in comprehensively solve sewage.
Summary of the invention
To solve the problems, such as that existing sewage water treatment method complicated pollutant component, the present invention can not provide in comprehensively solve sewage
A kind of method using ABS microbial bacterial agent processing sewage.
Technical solution of the present invention:
A method of sewage being handled using ABS microbial bacterial agent, sewage successively passes through septic tank or collecting pit, grid
Net, conditioning tank, biological tank and sludge-water separating system；
A certain amount of ABS microbial bacterial agent is added in the septic tank or collecting pit, sewage controls after entering septic tank
Certain aeration quantity carries out the primary degradation of sewage, and processing is after a certain period of time by sewage by septic tank or collecting pit discharge and through institute
Enter the conditioning tank after stating grid net filtration, sewage is discharged into the biological tank after conditioning tank completes pH value adjustment；
A certain amount of ABS microbial bacterial agent has been added in the biological tank, is started to be aerated after sewage is full of biological tank, be controlled
It makes certain aeration quantity and carries out the degradable of sewage, sewage is discharged into mud-water separation system by biological tank after a certain period of time by processing
System, sewage completes mud-water separation in sludge-water separating system to be discharged；
The ABS microbial bacterial agent includes saprophytic sac fungus, the mould basidiomycetes of short stalk, malic acid acetobacter, withered grass gemma
Bacillus, bacillus licheniformis, Paenibacillus polymyxa, clostridium, bifidobacterium longum, bifidobacterium breve, nitrobacteria,
Denitrifying bacteria, pink mold cyst bacterium and streptomyces.
Further, in the septic tank or collecting pit the dosage of ABS microbial bacterial agent by septic tank liquid amount for the first time
Add 0.5kg/m3, monthly periodically add 0.025kg/m3。
Further, ABS microbial bacterial agent is attached on single creature medium in the septic tank or collecting pit, the list
Bamboo is cut into the monolithic that width is 1~3cm, spaced group of front and back sides by the natural renewable bamboo of one Biomedia materials
In a row, high 210cm, wide 98cm, 7 rows are one group, every row's spacing 15cm, six roots of sensation fine bamboo rod fix every row and form cuboid, outside
Exterior feature diagonally installs aeration head having a size of 105cm × 98cm × 210cm, every group of both ends.
Further, the septic tank or the aeration control on collecting pit daytime are every square metre of 45~50L/h, evening
Aeration control is every square metre of 0~20L/h, and sewage is 3~5h in the processing time of the septic tank or collecting pit.
Further, the grid screen specification that grid processing uses be rake teeth grid gap be 1mm, 3mm, 5mm, 10mm,
20mm, 30mm, 40mm or 50mm.
Further, the pH value of sewage water is adjusted in conditioning tank to 6.5~7.8.
Further, the dosage of ABS microbial bacterial agent is added for the first time by biological tank liquid amount in the biological tank
0.5kg/m3, monthly periodically add 0.025kg/m3。
Further, the biological tank aeration time is that continuous aeration, continuous aeration amount are every square metre of 45~50L/ for 24 hours
H, sewage are 3~5h in the processing time of the biological tank.
Further, the ABS microbial bacterial agent in the biological tank is attached on filler combination Biomedia, the filler
Combination Biomedia includes commercially available filling for sewage treatment and Biomedia, the natural renewable bamboo of the Biomedia materials
Son is weaved bamboo baskets by specification 70cm × 49cm × 35cm, and bamboo chip front and back sides are spaced, is bundled to form specification bamboo basket biology with rattan
Medium, every three bamboo baskets form one group of Biomedia, and two baskets are engaged, and the bamboo basket that microbial bacterial agent and filler are housed is placed in centre.
Further, the ABS microbial bacterial agent in the biological tank is attached on set baby's formula Biomedia, the set baby formula
The natural renewable bamboo of Biomedia materials, by specification 70cm × 49cm × 35cm, 66cm × 45cm × 34cm, 62cm ×
41cm×33cm、58cm×37cm×32cm、54cm×33cm×31cm、50cm×29cm×30cm、46cm×25cm×
29cm weaves bamboo baskets, and bamboo chip front and back sides are spaced, is bundled to form specification bamboo basket Biomedia with rattan, descending seven bamboo baskets
Being nested together is half group, and two and half groups are buckled together to form one group of bamboo basket Biomedia, contour dimension be 70cm × 49cm ×
70cm, each layer bamboo basket gap filling microbial bacterial agent.
Further, the Activiation method of the ABS microbial bacterial agent is disposable for the ABS microbial bacterial agent that will be put into for the first time
It uniformly puts into septic tank or collecting pit or biological tank, is intake using stage adjustment, to reduce the impact to microorganism, operation
The 1/3 of normal inflow is opened within first day, opens within second day 2/3, third day standard-sized sheet closes water inlet and water outlet, keeps aeration shape
State 120h, aeration quantity be every square metre of 45~50L/h, aeration time be activate microorganism and be attached to Biomedia with/
Or be proliferated on filler, microbial flora is then completed to activate after reaching active state and system stabilization.
Beneficial effects of the present invention:
Method provided by the invention using ABS microbial bacterial agent processing sewage is different from traditional wastewater processing mode, this
Invention is the widely used effective microbe processing technique of a comprehensive composition, and microorganism used therefor microbial inoculum is by a variety of fungies, bud
The comprehensive microbial flora that spore bacterium, bacterium and actinomyces are combined, have biochemical characteristic, it is aerobic can be anti-with slot with anaerobism
It answers, realize polynary fermentation and respiration；Vitality is strong, resistance to low and high temperature up to 0~100 DEG C, it is strong alkali-acid resistance, resistance to hyperoxia, resistance to low
Oxygen, can keep bioactivity for a long time, and storage stability 2~3 years.
Sewage water treatment method of the present invention is different from general biologic treating technique and only more effectively degrades to SS, BOD, COD, to excrement
Escherichia coli, ammonia nitrogen etc. do not have effect, and processing method of the present invention contains in BOD, COD, colibacillus of excrement, pathogenic bacteria, virus, ammonia nitrogen
Treatment effect in high environment is measured to have outstanding performance, can in 3~5 hours to Escherichia coli, pathogenic microorganisms, BOD, COD, TN and
PN completes digestion degradation.Without chlorine dioxide, ozone, Disinfection Effect is can be realized in the sterilizing parts such as ultraviolet light, can prevent dioxy
Water body again foul to form secondary pollution by blackening after the disinfectants such as change chlorine lead to the problem of noxious odor pollution environment and reduction, together
Shi Jieyue large amount of sewage handles fund.
Sewage water treatment method of the present invention is different from continuous activated sludge process and is spatially arranged what different facilities were fixed
Continuous operation, the present invention carry out various purposes not in single reaction tank in differential responses pond on the same time
Different operation.Intermittent duty mode of the invention and many industry sewages generate the period than more consistent, can more efficiently into
Row sewage treatment.
Detailed description of the invention
Fig. 1 is the process flow chart provided by the invention that sewage is handled using ABS microbial bacterial agent.
Specific embodiment
Below with reference to embodiment, the following further describes the technical solution of the present invention, and however, it is not limited to this, all right
Technical solution of the present invention is modified or replaced equivalently, and without departing from the spirit and scope of the technical solution of the present invention, should all be contained
Lid is within the protection scope of the present invention.
Embodiment 1
A kind of method using ABS microbial bacterial agent processing sewage is present embodiments provided, sewage successively passes through septic tank
Or collecting pit, grid, conditioning tank, biological tank and sludge-water separating system；
A certain amount of ABS microbial bacterial agent is added in the septic tank or collecting pit, sewage controls after entering septic tank
Certain aeration quantity carries out the primary degradation of sewage, and processing is after a certain period of time by sewage by septic tank or collecting pit discharge and through institute
Enter the conditioning tank after stating grid net filtration, sewage is discharged into the biological tank after conditioning tank completes pH value adjustment；
A certain amount of ABS microbial bacterial agent has been added in the biological tank, is started to be aerated after sewage is full of biological tank, be controlled
It makes certain aeration quantity and carries out the degradable of sewage, sewage is discharged into mud-water separation system by biological tank after a certain period of time by processing
System, sewage completes mud-water separation in sludge-water separating system to be discharged；
The ABS microbial bacterial agent includes saprophytic sac fungus, the mould basidiomycetes of short stalk, malic acid acetobacter, withered grass gemma
Bacillus, bacillus licheniformis, Paenibacillus polymyxa, clostridium, bifidobacterium longum, bifidobacterium breve, nitrobacteria,
Denitrifying bacteria, pink mold cyst bacterium and streptomyces.
Embodiment 2
Present embodiments provide a kind of ABS microbial bacterial agent, including saprophytic sac fungus, the mould basidiomycetes of short stalk, apple vinegar
Acidfast bacilli, bacillus subtilis, bacillus licheniformis, Paenibacillus polymyxa, clostridium, bifidobacterium longum, short pair
Discrimination bacillus, nitrobacteria, denitrifying bacteria, pink mold cyst bacterium and streptomyces.
The strain source and function of each microorganism provided in this embodiment:
The latin name of saprophytic sac fungus is SaprophyticAscomycetes, is purchased from hundred Ou Bowei biotechnology of Beijing
Co., Ltd, product number bio-089576.
The latin name of the short mould basidiomycetes of stalk is Aureobasidiumpullulans var.melanigenum, purchased from north
Hundred Ou Bowei Bioisystech Co., Ltd of capital, product number bio-85564.
Saprophytic sac fungus and the short mould basidiomycetes of stalk play an important role in the ecosystem and organic substance circulation, are that can divide
Special chemical component, organic acid or enzyme are secreted to the fungi of efficient-decomposition plant and animal residues.
The latin name of HAAP-1 malic acid acetobacter is Acetobacterium malicum, is purchased from hundred Ou Bo of Beijing
Big Bioisystech Co., Ltd, product number bio-103511.
Malic acid acetobacter is a kind of acidophilic bacteria, is played a significant role in the ecosystem, can degrading plant residuum
Polymer, the metabolism for participating in ferrikinetics and carbohydrate, have decomposition to heavy metal, have removal effect to nitrogen, phosphorus.
The latin name of bacillus subtilis is Bacillus subtilis, is purchased from the big life of hundred Ou Bo of Beijing for totally 15 plants
Object Technology Co., Ltd., product number be followed successively by bio-103318,103217,103209,103208,103192,103190,
103189,103188,103185,103183,103182,103181,103180,103177 and 103176.
The latin name of bacillus licheniformis is Bacillus licheniformis, is purchased from hundred Ou Bo of Beijing for totally 5 plants
Big Bioisystech Co., Ltd, product number are followed successively by Bio-101680,101679,101678,101673 and 101672.
The latin name of Paenibacillus polymyxa is Paenibacillus polymyxa, is purchased from hundred Europe, Beijing for totally 5 plants
Bo Wei Bioisystech Co., Ltd, product number are followed successively by bio-101852,093897,087219,096651 and 61098.
The latin name of clostridium is Bacillus fusiformis, is purchased from the big life of hundred Ou Bo of Beijing for totally 3 plants
Object Technology Co., Ltd., product number are followed successively by bio-61557,61554 and 58756.
Bacillus subtilis, bacillus licheniformis, Paenibacillus polymyxa, clostridium are suitable with stronger environment
Should be able to power and decomposing organic pollutant ability, bacillus thallus is big, more four times greater than general sex pheromone, takes up space excellent
Gesture is able to suppress the growth and breeding of pathogenic microorganism.
Bacillus subtilis organic matter decomposition power is strong, and proliferation while can release the amylase of high activity, and hardly possible is decomposed
Macromolecular substances resolve into available small-molecule substance, and generate metabolism product abundant, synthesize a variety of organic acids,
The substances such as enzyme, physiological activity and other a variety of nutrients for being easy to be utilized.Can decompose generate foul gas organic substance,
Organic sulfur compound, organic nitrogen etc. substantially improve the environment in place.
The latin name of bifidobacterium longum is Bifidobacterium longum, is purchased from hundred Ou Bowei of Beijing for totally 5 plants
Bioisystech Co., Ltd, product number are followed successively by bio-67291,67288,67276,67263 and 67261.
The latin name of bifidobacterium breve is Bifidobacterium breve, is purchased from the big life of hundred Ou Bo of Beijing for totally 4 plants
Object Technology Co., Ltd., product number are followed successively by bio-67242,67233,67230 and 67125.
Bifidobacterium longum and bifidobacterium breve are beneficial bacteria of intestinal tract, have and inhibit the micro- life of the cause of diseases such as spoilage organisms and Escherichia coli
The ability of object.
The latin name of nitrobacteria is Nitrobacter sp., is purchased from hundred Ou Bowei Bioisystech Co., Ltd of Beijing,
Product number is bio-75326.
The latin name of denitrifying bacteria is Achromobacter denitrificans, is purchased from the big life of hundred Ou Bo of Beijing
Object Technology Co., Ltd., product number bio-103450.
Nitrobacteria can utilize the oxygen in nitric acid, oxidation of organic compounds matter, and denitrifying bacteria can convert nitrogen for nitrate nitrogen
Gas rather than ammoniacal nitrogen, the two have stronger Scavenging activity to pollutant containing nitre in sewage.
The latin name of pink mold cyst bacterium is Streptosporangiaceae sp., is purchased from hundred Ou Bowei of Beijing for totally 8 plants
Bioisystech Co., Ltd, product number be followed successively by bio-097102,17510 (red pink mold cyst bacterium), 17476,16169,
16167,00259 (yellowish pink pink mold cyst bacterium), 16191 and 16101.
The latin name of streptomyces is Streptomyces sp., is purchased from hundred Ou Bowei biotechnology of Beijing for totally 15 plants
Co., Ltd, product number be followed successively by bio-098292,098291,096957,094051,094049,84769,83209,
81969,79845,75322,75307,75230,66311,66306 and 80896.
Pink mold cyst bacterium and streptomyces can inhibit the growth of pathogenic microorganism using several kinds of carbon source as nutriment
And breeding, decompose the Determination of multiple metal elements in sewage.
The preparation method of ABS microbial bacterial agent provided in this embodiment, steps are as follows:
Step 1: preparation A microbial inoculum, B microbial inoculum and S microbial inoculum:
(1) A microbial inoculum:
By the saprophytic sac fungus of goods number listed by the present embodiment, the mould basidiomycetes of short stalk and HAAP-1 malic acid acetobacter
Preservation of bacteria strain thaws, and is seeded in nutrient agar respectively, and culture makes rejuvenation of spawn at a temperature of 30~33 DEG C, selects healthy and strong
Bacterium colony continue passage 2~3 times, the saprophytic sac fungus activated, the mould basidiomycetes of short stalk and HAAP-1 malic acid acetobacter
Slant culture.
The preparation method of bacteria suspension: the saprophytic sac fungus slant culture after taking activation adds 1~2mL of sterile water to wash lawn
Under suspension is made, this suspension is seeded in the flat culture bottle for filling nutrient agar with aseptic straw, uniformly spreads out cloth,
30~33 DEG C of cultures 22~for 24 hours, a little smear of lawn is taken, gram staining microscopy there should be saprophytic 85% or more sac fungus, with going out
Bacterium water 10mL washes down the saprophytic sac fungus lawn in flat culture bottle outer inclined-plane, and saprophytic ascus bacteria suspension is made.
Bacteria suspension preparation method and the saprophytic sac fungus bacteria suspension of short stalk mould basidiomycetes and HAAP-1 malic acid acetobacter
Preparation method is identical.
The viable count of saprophytic sac fungus bacteria suspension obtained is 1.2~2.3 × 108Cfu/ml, the short mould basidiomycetes bacterium of stalk are outstanding
The viable count of liquid is 1.0~2.0 × 108The viable count of cfu/ml, HAAP-1 malic acid acetobacter bacteria suspension is 1.7~3.0
×108cfu/ml。
The bacteria suspension of above-mentioned three kinds of bacterium is inoculated in culture medium A respectively by inoculum concentration 30%, is trained at a temperature of 23~26 DEG C
It supports to viable bacteria density OD600 and reaches 0.6~0.8, the bacteria suspension of three kinds of bacterium is mixed to get A microbial inoculum, A microbial inoculum contains viable count
4.0~8.0 × 108Cfu/ml, optimal pH 6.5~8.
The formula of culture medium A are as follows: peptone 15g, agar 20g, maltose 45g, corn flour 10g, wheat bran 10g, water
1000ml。
(2) B microbial inoculum:
By 15 bacillus subtilis of goods number listed by the present embodiment, 5 bacillus licheniformis, class bud is glued 5 plants more
Spore bacillus, 3 plants of clostridium, 5 plants of bifidobacterium longums, 4 plants of bifidobacterium breves, 1 plant of nitrobacteria and 1 plant of denitrifying bacteria
Preservation of bacteria strain thaws, and is seeded in nutrient agar respectively, and culture makes rejuvenation of spawn at a temperature of 30~33 DEG C, selects healthy and strong
Bacterium colony continue passage 2~3 times, the slant culture of each strain activated.
The preparation method of bacteria suspension: slant culture each one of 15 bacillus subtilis after taking activation, respectively plus
1~2mL of sterile water, which washes down lawn, is made suspension, with aseptic straw 15 suspensions is seeded to 15 respectively and fills nutrient agar
In the flat culture bottle of culture medium, cloth is uniformly spread out, cultivate 22 at 30~33 DEG C~for 24 hours, with aqua sterilisa 10mL by 15 flat culture bottles
15 bacillus subtilis lawns in outer inclined-plane are washed down, and the plastc ring of bacillus subtilis is made.
Bacillus licheniformis, Paenibacillus polymyxa, clostridium, bifidobacterium longum, bifidobacterium breve, nitrification are thin
The preparation method of bacterium and denitrifying bacteria bacteria suspension is identical as the preparation method of bacillus subtilis bacteria suspension, wherein lichens gemma
Using the flat culture bottle of 5 bacillus licheniformis, one each, Paenibacillus polymyxa plastc ring uses 5 to bacillus plastc ring
The strain flat culture bottle of Paenibacillus polymyxa is one each, clostridium plastc ring uses 3 plants of flat trainings of clostridium
Support bottle is one each, bifidobacterium longum plastc ring using 5 plants of flat culture bottles of bifidobacterium longum it is each one and bifidobacterium breve it is mixed
Combined bacteria suspension uses 4 plants each one, the flat culture bottle of bifidobacterium breve.
The viable count of bacillus subtilis bacteria suspension obtained is 2.8~3.2 × 108Cfu/ml, Bacillus licheniformis
The viable count of suspension is 1.0~2.0 × 108Cfu/ml, Paenibacillus polymyxa bacteria suspension viable count be 1.0~1.5 ×
108Cfu/ml, clostridium bacteria suspension viable count be 1.0~1.2 × 108The work of cfu/ml, bifidobacterium longum bacteria suspension
Bacterium number is 1.0~2.0 × 108Cfu/ml, bifidobacterium breve bacteria suspension viable count be 1.0~1.5 × 108Cfu/ml, nitrification are thin
The viable count of bacterium bacteria suspension is 1.0~1.8 × 108Cfu/ml, denitrifying bacteria bacteria suspension viable count be 1.0~1.8 ×
108cfu/ml。
Above-mentioned eight kinds of bacteria suspensions are inoculated in culture medium B respectively by inoculum concentration 50%, cultivated at a temperature of 28~30 DEG C to
Viable bacteria density OD600 reaches 0.6~0.8, and the bacteria suspension of eight kinds of bacterium is mixed to get B microbial inoculum, B microbial inoculum contain viable count 9.0~
18.0×108Cfu/ml, optimal pH 6.5~8.
The formula of culture medium B are as follows: beef extract 3g, peptone 10g, agar 15g, maltose 20g, corn flour 10g, sodium chloride
5g, water 1000ml.
(3) S microbial inoculum:
8 plants of pink mold cyst bacterium of goods number listed by the present embodiment and 15 streptomycete category preservation of bacteria strain are thawed, are connect respectively
Kind is into nutrient agar, and culture makes rejuvenation of spawn at a temperature of 30~33 DEG C, selects healthy and strong bacterium colony and continues passage 2~3
It is secondary, the slant culture of 8 plants of pink mold cyst bacterium and 15 streptomycetes being activated.
The preparation method of bacteria suspension: 8 plants of pink mold cyst bacterium slant cultures each one after taking activation, respectively plus sterile water 1
~2mL, which washes down lawn, is made suspension, with aseptic straw 8 suspensions is seeded to 8 respectively and fills nutrient agar
In flat culture bottle, uniformly spread out cloth, 30~33 DEG C cultivate 22~for 24 hours, will be in 8 flat culture bottle outer inclined-planes with aqua sterilisa 10mL
8 plants of pink mold cyst bacterium lawns are washed down, and the plastc ring of pink mold cyst bacterium is made.
The preparation method of pink mold cyst bacterium bacteria suspension is identical as the preparation method of pink mold cyst bacterium bacteria suspension, pink mold cyst bacterium Mixed Microbes
Suspension uses each one, the flat culture bottle of 15 streptomycetes.
The viable count of pink mold cyst bacterium bacteria suspension obtained is 1.0~2.0 × 108The work of cfu/ml, streptomyces bacteria suspension
Bacterium number is 2.0~3.0 × 108cfu/ml。
Above two bacteria suspension is inoculated in culture medium S respectively by inoculum concentration 30%, cultivates and lives at a temperature of 25~36 DEG C
Strain density OD600 reaches 0.6~0.8, the bacteria suspension of two kinds of bacterium is mixed to get S microbial inoculum, S microbial inoculum contains viable count 3.0~5.0
×108Cfu/ml, optimal pH 6.5~8.
The formula of culture medium S are as follows: soluble starch 4g, potassium nitrate 0.2g, dipotassium hydrogen phosphate 0.1g, sodium chloride 0.1g, sulphur
Sour magnesium 0.1g, ferrous sulfate 0.002g, agar 4g, water 1000ml.
Step 2: preparation ABS microbial bacterial agent:
13:70:17 is mixed A microbial inoculum, B microbial inoculum and the S microbial inoculum that step 1 is obtained by volume, is inoculated with by inoculum concentration 30%
It in semisynthetic medium, ferments for 24 hours under conditions of 32 DEG C of temperature and ventilatory capacity are 1.2vvm, in volatilization removal fermentation liquid
Moisture, obtains ABS microbial bacterial agent, and ABS microbial bacterial agent contains viable count 18.0~35.0 × 108Cfu/ml, optimal pH 6.5
~8.
The formula of semisynthetic medium are as follows: beef extract 5g, potato 15g, maltose 10g, magnesium sulfate 0.1g, potassium nitrate
0.1g, water 1000ml.
ABS microbial bacterial agent provided in this embodiment is as its advantage of third generation microbial bacteria are as follows:
Near-zero mud and sewage processing technique, 200 grams of sludge of every hundred tons of aquatic products, sludge in wastewater treatment process of assaulting fortified position at one stroke
Discharge pain；Have the polluters such as superpower removal BOD, COD, SS, ammonia nitrogen, phosphorus, effective percentage reaches 95-97% or more；Water producing tank
Water outlet can accomplish III class water quality discharge standard of earth's surface completely；Coping with dyestuff and dyeing and finishing wastewater and other has difficult elimination color useless
Water, dispensing can direct bleachings；Have significant deodorizing effect, eliminates NH3、P、H2The ability of S and organic acid is superpower；Superpower is numerous
It grows and adaptability, the Sewage Environment of the following complexity can be coped with；Degrading pesticide, Polychlorinated biphenyls, plasticiser, synthetic detergent, life
Object synthetic plastic etc. synthesizes chemical combination pollutant；Inhibit virus, germ and helminth.Inhibit algae reproduction, purifying water body and water colour；
Remove the heavy metal pollution in sanitary sewage, such as zinc, manganese, iron, chromium.
The principle of ABS microbial bacterial agent processing sewage provided in this embodiment is:
Organic pollution materials are resolved into water and two using the dissolved oxygen (DO) in water by aerobic microorganism sewage treatment strain
Carbonoxide, or be converted into the nutriment of sewage treatment microorganism, and bred using these nutrients, process just can be with
Pollution degradation substance, achievees the purpose that decontamination deodorization, and such facture is known as aerobic processing.
General anerobe processing sewage is to utilize nitrate by nitrate reduction in the environment of no dissolved oxygen
In oxygen, carry out denitrification reaction, so that its is generated nitrogen, such side is widely used in the wastewater treatment containing nitrogen.And acid generates
Bacterium, general anerobe, acidification reaction early period being usually used in absolute anaerobe sewage treatment engineering method.
Absolute anaerobe processing is to generate bacterium using acid to carry out acidification reaction, by the carbohydrate or protein point in sewage
Solution is at monosaccharide, Amino acid or lower fatty acid, organic acid.Generating bacterium again with absolute anerobe-acetic acid will be in sewage
Monosaccharide, Amino acid or organic acid resolve into acetic acid.Acetic acid is finally decomposed with absolute anerobe-Methane producing bacteria again
Generate methane.
Most sewage treatments with microorganism using pollutant as food, such as carbohydrate, protein-based and fats
Equal pollutants can become the nutrient of own growth breeding by various sewage treatment microorganism decompositions.And utilize gemma bar
Foul gas hydrogen sulfide can be converted to element sulphur required for own growth, and then achieve the purpose that deodorization by bacterium.
The microorganism sewage water processing strain polysaccharide emplastic that has itself, can be using adsorbing the pollution in environment
Object, such characteristic are often applied to the absorption to heavy metal ion.
When entering in sewage by peculiar microorganism sewage treatment flora, the dominant bacteria in environment can be become, can be inhibited
After growth of pathogen and spoilage organisms, such as lactic acid bacteria etc. become dominant bacteria, the growth of Escherichia coli etc. in environment can suppress,
To reduce the generation of the stinks such as ammonia, the environment in place is substantially improved.
Embodiment 3
A kind of ABS microbial bacterial agent of preparation of embodiment 2 is present embodiments provided in septic tank or collecting pit or biological tank
Activiation method:
The ABS microbial bacterial agent put into for the first time is disposably uniformly put into septic tank or collecting pit or biological tank, is used
Stage adjustment water inlet runs to reduce the impact to microorganism and opens within first day the 1/3 of normal inflow, opens within second day
2/3, third day standard-sized sheet is then shut off into water and water outlet, keeps aeration state 120h, and aeration quantity is every square metre of 45~50L/h,
Aeration time be activate microorganism and is attached on Biomedia and/or filler to be proliferated, detection water outlet in COD120, greatly
Below enterobacteria 1100.Monitoring is run with adaption systems, if system is stablized after about 8 days, is no longer needed to addition ABS microbial inoculum, is completed
Activation.
Embodiment 4
Present embodiments provide the ABS microbial bacterial agent that is prepared using embodiment 2 septic tank or collecting pit to sewage into
The method of row primary degradation:
Step 1: preparing and placing the single creature medium for attached microbial microbial inoculum:
Bamboo is cut into the monolithic that width is 1~3cm, front and back sides by the natural renewable bamboo of single creature medium materials
Spaced group in a row, high 210cm, wide 98cm, and 7 rows are one group, every row's spacing 15cm, six roots of sensation fine bamboo rod fix every row's composition
Cuboid, contour dimension are 105cm × 98cm × 210cm, and aeration head is diagonally installed at every group of both ends, according to the high low-key of sewage level
The height of whole every group of Biomedia.
Placement quantity of the single creature medium in septic tank or collecting pit by septic tank or collect pool volume determine, normally
Modes of emplacement is to be paved with septic tank or collecting pit bottom of pond.
Step 2: adding and activating ABS microbial bacterial agent:
The ABS microbial bacterial agent of the preparation of embodiment 2 is added in septic tank or collecting pit, dosage presses septic tank liquid amount
0.5kg/m is added for the first time3, monthly periodically add 0.025kg/m3, ABS microbial inoculum is activated by the Activiation method that embodiment 3 provides.
Step 3: the pollutant in primary degradation sewage:
Sewage controls the aeration quantity on daytime after entering septic tank as every square metre of 45~50L/h, and the aeration quantity in evening is every
Square metre 0~20L/h, if aeration stops at night, dwell time is 2~3h.When daytime, water consumption was big, sewage with continue into
The mode of water enters septic tank or collecting pit, and the processing time is 3~5h；Water consumption hour in the evening, sewage can in septic tank or
10h is stopped in collecting pit.This stage microbial bacterial agent carries out primary degradation to the pollutant in sewage, including removes odors and just
NH in grade degradation sewage3- N, TN and vegetable and animals oils, are discharged septic tank for sewage after the completion of processing.
Embodiment 5
It is degradable to sewage progress in biological tank to present embodiments provide the ABS microbial bacterial agent for preparing embodiment 2
Method:
It is situated between Step 1: preparing and placing for the filler combination Biomedia or set baby's formula biology of attached microbial microbial inoculum
Matter:
Filler combination Biomedia includes commercially available filling for sewage treatment and Biomedia, and the Biomedia materials are natural
Renewable bamboo, weave bamboo baskets by specification 70cm × 49cm × 35cm, bamboo chip front and back sides are spaced, bundled to form rule with rattan
Lattice bamboo basket Biomedia, every three bamboo baskets form one group of Biomedia, and two baskets are engaged, and centre, which is placed, to be equipped with microbial bacterial agent and fill out
The bamboo basket of material.
The natural renewable bamboo of baby's formula Biomedia materials is covered, by specification 70cm × 49cm × 35cm, 66cm × 45cm
×34cm、62cm×41cm×33cm、58cm×37cm×32cm、54cm×33cm×31cm、50cm×29cm×30cm、
46cm × 25cm × 29cm weaves bamboo baskets, and bamboo chip front and back sides are spaced, is bundled to form specification bamboo basket Biomedia with rattan, by big
Being nested together to small seven bamboo baskets is half group, and two and half groups are buckled together to form one group of bamboo basket Biomedia, and contour dimension is
70cm × 49cm × 70cm, each layer bamboo basket gap filling microbial bacterial agent.
The quantity that filler combination Biomedia or set baby's formula Biomedia are placed determines by biological pool volume, normal placement side
Formula is to be paved with biological tank bottom of pond.
Step 2: adding and activating ABS microbial bacterial agent:
The ABS microbial bacterial agent of the preparation of embodiment 2 is added in biological tank, dosage is added for the first time by biological tank liquid amount
0.5kg/m3, monthly periodically add 0.025kg/m3, ABS microbial inoculum is activated by the Activiation method that embodiment 3 provides.
Step 3: water inlet:
Embodiment 4 through septic tank or the processed sewage of collecting pit, through rake teeth grid gap be 1mm, 3mm, 5mm, 10mm,
Enter conditioning tank after the grid net filtration of 20mm, 30mm, 40mm or 50mm, adjusts the pH value of sewage to 6.5 in conditioning tank
~7.8, sewage is then discharged into biological tank；
Step 4: the pollutant in degradable sewage:
Biological tank aeration time is that continuous aeration, continuous aeration amount are every square metre of 45~50L/h for 24 hours, and sewage is in biology
The processing time in pond is 3~5h.
Sewage is discharged into biological tank by the way of persistently intaking by method provided in this embodiment, in fill phase, biology
Play the role of balanced sewage quality, water to a certain extent in pond.ABS microbial bacterial agent has one to the fluctuation of water quality, water
Fixed adaptability.
Method provided in this embodiment using uninterrupted aeration for 24 hours, be aerated under environment herein aerobic, anaerobic reaction simultaneously into
Row, organic matter are had begun during water inlet by a large amount of oxidative degradations.
Method provided in this embodiment can be at runtime according to the growth characteristic of ABS microbial bacterial agent and to having in sewage
The resolution process target of machine object, inorganic matter adjusts aeration mode and water intake velocity, by controlling the environment of fill phase, realizes
The dirt of various complicated pollutant components and stubborn and stupid property in the case that microbial bacterial agent is constant in biological tank in degradable sewage
Water.
Pollutant is removed in biological tank by the degradation of ABS microbial flora, including Escherichia coli, cause a disease
Microorganism, BOD, COD, TN and PN.When according to the water quality of raw water and discharge standard concrete condition determines the processing in degradable stage
Between, generally 3~5h.
Anaerobic-aerobic is the characteristic of ABS microbial bacterial agent with slot, and the realization of anaerobic bacteria and aerobic bacteria different function is by exposing
What tolerance determined.According to the sewage of different industries, continuous aeration mode, intermittent aeration mode, peak valley formula aeration mode are selected,
Intermittent aeration amount is the 60% of continuous aeration amount, and peak valley formula aeration quantity is the 60~90% of continuous aeration amount.
Continuous aeration mode is mainly used for biological tank, there is distance between each aeration head, and aerobic bacteria is just centered around more than oxygen
The characteristics of place is bred, anaerobic bacteria stays in the thin location resolution harmful substance of oxygen, ABS microbial flora is exactly oneself
Suitable place breeding can be found.
Intermittent aeration mode is mainly used for septic tank or collecting pit, and daytime, the big aeration of inflow reached every square metre of 50L/
Per hour, the small aeration of evening inflow arrives every square metre of 0~20Lh.
Peak valley formula aeration mode is mainly used for the improvement biological tank of municipal sewage treatment, i.e. one anaerobism, aerobic merging pond,
Daytime is every square metre of 45L/h, is every square metre of 20L/h at night, if water level reaches 2~4 meters of aeration quantity and doubles.
Bacillus accounts for 70% in ABS microbial bacterial agent, and metabolism, breeding are fast, is proliferated 10 times, standard bacteria four hours within four hours
6 times can only be bred.The general four times greater number of pathogeny bacterium molecule of volume ratio, take up space advantage, inhibits the growth and breeding of harmful bacteria,
ABS microbial bacterial agent handles the alternating of the method fill phase of sewage and the anoxic of the stage of reaction or anaerobic and aerobic state, energy
Inhibit the excessive breeding of obligate aerobic der Pilz, and controls expansion.
One important advantage of the method for ABS microbial bacterial agent processing sewage is that operator can be protected by controlling related condition
Hold the selectivity of ABS microbial bacterial agent.In a complete process cycle, ABS microbial bacterial agent select the big of bucklingization these
Selection briquetting includes the availability of oxygen and matrix.Although also will appear in these selection pressures in some traditional continous way systems
A certain situation, and ABS microbial bacterial agent processing sewage method have well selection and expansion capability, allow microorganism
It is grown in superior environment.
Nitrogen in sewage enters system in the form of organic amino and ammonia nitrogen, is removed from system in the form of nitrogen.Ammonia nitrogen
The process for being converted into nitrogen is divided into nitrification and denitrification process.Nitrifying process is carried out under conditions of dissolved oxygen abundance, anti-nitre
Change process is occurred in the case where anoxic.To remove the nitrogen in sewage, as long as the operation to treatment plant is simply adjusted,
Regulating cycle and aeration time, and do not have to carry out big transformation to the structures for the treatment of plant.
Biological phosphate-eliminating is firstly the need of an anaerobic phase, and without the nitrogen of dissolved oxygen and oxidation state, existing simultaneously degradable has
Machine matter promotes sludge to absorb excessive phosphorus in aerobic stage, i.e. high-solubility oxygen concentration.From anti-before next anaerobic phase starts
Ying Chizhong excludes a certain amount of excess sludge.The flexibility of the method for ABS microbial bacterial agent processing sewage changes can pass through
Become operational mode to meet these conditions.The operation program of dephosphorization is completed in an ABS system are as follows: water inlet, aeration, precipitating row
Mud, draining.
Embodiment 6
Present embodiments provide a kind of microculture restoration methods of ABS microbial bacterial agent for sewage treatment:
Due to the specific function of ABS microbial bacterial agent, the pollutant in raw water is all decomposed as the nutriment of bacterium to disappear
Change.The separation of solid and liquid of precipitating link is just not present, and flowing water advances to next processing links, and sludge quantity is seldom.Raw water is come
Aeration quantity is reduced to original 30%, ABS microbial bacterial agent and enters static culture convalescence when amount stops, and water level is always held at
Lowest water level when circulation starts, the water level will also have certain protection height from sludge blanket.What biological bottom of pond portion settled down
Sludge largely flows to the returned sludge in next processing links or as next cycle.
Embodiment 7
Present embodiments provide a kind of mud-water separation method after the completion of microbiological treatment:
Sludge-water separating system is entered by the sewage that biological tank is discharged, sludge-water separating system is the floating suction of pressure in the present embodiment
Tank floats cucurbitula body, pressure pipeline and filter plate by pressure and forms.Sewage enters pressure and floats in cucurbitula, under 1.5 atmospheric pressure,
Make sewage by filter plate, impurity, suspended matter and baregin is effectively removed, to achieve the purpose that mud-water separation.
It is known as standby process during starting after precipitating to the next period.In the more case systems of biological tank, standby mesh
Be the whole cycle for providing the time for a reaction system before turning to another unit to complete it.It is standby be not one must
The step of needing, removes excess sludge during standby according to technique and processing intent.The length of standby phase is determined by processing water
It is fixed.
In precipitating-a advantage of the method for ABS microbial bacterial agent processing sewage is to stopped water-in and water-out, also stop
Gas, takes full advantage of static state deposition principle, can get separation faster in this way, can also avale more solids.Traditional company
The precipitation unit of continuous formula system can not stop water-in and water-out, therefore precipitating carries out in a dynamic condition.ABS microbial bacterial agent
Another advantage of the method for processing sewage is its flexibility, thus it is possible to vary the time of precipitation process.When flow is larger, precipitating
Time can be reduced to minimum time necessary to solid separates to shorten the time of whole cycle, handle bigger flow, such as
It is necessary to strain water to begin in precipitating.Legacy system does not have this flexibility then.
The present embodiment does not need the equipment such as the sedimentation basin that must be arranged in continuous activated sludge process, return sludge pump.Continuously
Activated sludge process is different facilities to be spatially arranged continuous operation is fixed, and in contrast, the present invention is not single
In reaction tank, and the different operation of various purposes is carried out on the same time in differential responses pond.Intermittent duty of the invention
Mode generates the period than more consistent with many industry sewages, can more efficiently carry out sewage treatment.It can be widely applied for
Medical treatment is lived, in Industrial Waste Water Treatments.Since ABS microbial bacterial agent processing sewerage takes up little area, horizontal layout is compact, energy
Enough it is widely used in large, medium and small urban wastewater treatment.
Embodiment 8
Distinguished with what embodiment 2-7 was provided using the method and traditional microbial inoculum SBR technology of ABS microbial bacterial agent processing sewage
The sewage generated to Beijing hospital is handled, and traditional microbial inoculum contains bacillus, Blastocystis and Micrococcus, processing knot
Fruit is shown in Table 1；
Table 1
It can be seen from data in table 1 it is provided by the invention using ABS microbial bacterial agent processing sewage method can be more
Pollutants, the elimination effects such as efficient COD, SS, BOD, Escherichia coli, pathogenic bacteria, ammonia nitrogen and the total phosphorus removed in sewage are obvious
Better than traditional microbiological microbial inoculum, especially in sewage Escherichia coli and pathogenic bacteria elimination effect it is significant.Production after processing
Water can be in line, Treated sewage reusing service society, is not required to be discharged into municipal sewage plant's progress two stage treatment, mitigate at municipal sewage
Manage pressure.
Claims (10)
1. a kind of method using ABS microbial bacterial agent processing sewage, which is characterized in that sewage successively passes through septic tank or collection
Pond, grid, conditioning tank, biological tank and sludge-water separating system；
A certain amount of ABS microbial bacterial agent is added in the septic tank or collecting pit, sewage controls centainly after entering septic tank
Aeration quantity carry out sewage primary degradation, processing after a certain period of time by sewage by septic tank or collecting pit discharge and through the lattice
Enter the conditioning tank after aperture plate filtering, sewage is discharged into the biological tank after conditioning tank completes pH value adjustment；
A certain amount of ABS microbial bacterial agent has been added in the biological tank, starts to be aerated after sewage is full of biological tank, control one
Fixed aeration quantity carries out the degradable of sewage, and sewage is discharged into sludge-water separating system by biological tank after a certain period of time by processing, dirty
Water completes mud-water separation in sludge-water separating system to be discharged；
The ABS microbial bacterial agent include saprophytic sac fungus, the mould basidiomycetes of short stalk, malic acid acetobacter, bacillus subtilis,
Bacillus licheniformis, Paenibacillus polymyxa, clostridium, bifidobacterium longum, bifidobacterium breve, nitrobacteria, anti-nitre
Change bacterium, pink mold cyst bacterium and streptomyces.
2. a kind of method using ABS microbial bacterial agent processing sewage according to claim 1, which is characterized in that describedization
The dosage of ABS microbial bacterial agent adds 0.5kg/m by septic tank liquid amount for the first time in manure pit or collecting pit3, monthly periodically mend
Add 0.025kg/m3。
3. a kind of method using ABS microbial bacterial agent processing sewage according to claim 2, which is characterized in that describedization
ABS microbial bacterial agent is attached on single creature medium in manure pit or collecting pit, single creature medium materials it is natural can
Bamboo is regenerated, bamboo is cut into the monolithic that width is 1~3cm, spaced group of front and back sides are in a row, high 210cm, wide 98cm, and 7
Row is one group, every row's spacing 15cm, the fixed every row of six roots of sensation fine bamboo rod form cuboid, contour dimension be 105cm × 98cm ×
Aeration head is diagonally installed at 210cm, every group of both ends.
4. a kind of method using ABS microbial bacterial agent processing sewage according to claim 3, which is characterized in that describedization
Manure pit or the aeration control on collecting pit daytime are every square metre of 45~50L/h, the aeration control in evening is every square metre 0~
20L/h, sewage are 3~5h in the processing time of the septic tank or collecting pit.
5. a kind of method using ABS microbial bacterial agent processing sewage according to claim 4, which is characterized in that the dirt
Water pH value is adjusted in conditioning tank to 6.5~7.8.
6. a kind of method using ABS microbial bacterial agent processing sewage according to claim 5, which is characterized in that the life
The dosage of ABS microbial bacterial agent adds 0.5kg/m by biological tank liquid amount for the first time in object pond3, monthly periodically add
0.025kg/m3。
7. a kind of method using ABS microbial bacterial agent processing sewage according to claim 6, which is characterized in that the life
Object pond aeration time is that continuous aeration, continuous aeration amount are every square metre of 45~50L/h for 24 hours, and sewage is at the place of the biological tank
The reason time is 3~5h.
8. a kind of method using ABS microbial bacterial agent processing sewage according to claim 7, which is characterized in that the life
ABS microbial bacterial agent in object pond is attached on filler combination Biomedia, and the filler combination Biomedia includes commercially available dirt
Water process filler and Biomedia, the natural renewable bamboo of Biomedia materials, by specification 70cm × 49cm ×
35cm weaves bamboo baskets, and bamboo chip front and back sides are spaced, is bundled to form specification bamboo basket Biomedia with rattan, every three bamboo baskets composition one
Group Biomedia, two baskets are engaged, and the bamboo basket that microbial bacterial agent and filler are housed is placed in centre.
9. a kind of method using ABS microbial bacterial agent processing sewage according to claim 7, which is characterized in that the life
ABS microbial bacterial agent in object pond is attached on set baby's formula Biomedia, set baby formula Biomedia materials it is natural can be again
Raw bamboo, by specification 70cm × 49cm × 35cm, 66cm × 45cm × 34cm, 62cm × 41cm × 33cm, 58cm × 37cm ×
32cm, 54cm × 33cm × 31cm, 50cm × 29cm × 30cm, 46cm × 25cm × 29cm weave bamboo baskets, and bamboo chip front and back sides are alternate
Arrangement, is bundled to form specification bamboo basket Biomedia with rattan, it is half group that descending seven bamboo baskets, which are nested together, two and half groups of buttons
One group of bamboo basket Biomedia is formed together, and contour dimension is 70cm × 49cm × 70cm, each layer bamboo basket gap filling microorganism
Microbial inoculum.
10. -9 any a kind of method using ABS microbial bacterial agent processing sewage according to claim 1, which is characterized in that
The Activiation method of the ABS microbial bacterial agent be will the ABS microbial bacterial agent that put into for the first time disposably uniformly put into septic tank or
In collecting pit or biological tank, intake using stage adjustment, to reduce impact to microorganism, run open within first day normally into
The 1/3 of water, opens 2/3 for second day, and third day standard-sized sheet is then shut off into water and water outlet, keeps aeration state 120h, aeration quantity
For every square metre of 45~50L/h, aeration time is so that microorganism is activated and is proliferated for 24 hours, microbial flora reach active state and
System is then completed to activate after stablizing.
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