CN109813917A - A kind of calprotectin detection kit and its test method - Google Patents
A kind of calprotectin detection kit and its test method Download PDFInfo
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- CN109813917A CN109813917A CN201910131322.2A CN201910131322A CN109813917A CN 109813917 A CN109813917 A CN 109813917A CN 201910131322 A CN201910131322 A CN 201910131322A CN 109813917 A CN109813917 A CN 109813917A
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- sample
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- calprotectin
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Abstract
The present invention provides a kind of calprotectin detection kit and its test method, is related to medical domain.Including shell, sample pad, colored particles/fluorescent particle conjugate pad, nitrocellulose filter and water absorption pad are fixed in the inner cavity of the shell, the front of the shell simultaneously offers sample well right above sample pad, the front of the shell simultaneously offers watch window right above nitrocellulose filter, the inside of the shell is additionally provided with transparent partition, and the inner cavity of shell is divided into two not connected spaces by the partition.Compare the prior art, the present invention passes through setting dropper, and feeding pipe is set in the inside of shell, compared to more traditional kit, during sample is added dropwise to kit inside, user does not have to worry sample water clock, the situation that sample is not added dropwise to accurately inside kit will not occurs and occur.
Description
Technical field
The present invention relates to medicine technology field, specially a kind of calprotectin detection kit and its test method.
Background technique
Calprotectin (Calprotectin) is calcium, the zinc-binding protein that a molecular weight is 36kD, by two molecular weight
The light chain for being 8kD for the heavy chain of 14kD and a molecular weight is formed with the calcium-binding protein heterotrimer being covalently keyed, often
Chain is in combination with two Ca2+, to heat resistance and enhance water-disintegrable characteristic, protein structure is by 2 subunit S-
The compound of 100A8 and S-100A9 composition.It is distributed in the granulocyte of human body, monocyte, epithelial cell, keratinocyte and
It is protective protein in various tissues and body fluid.
Calprotectin is the prediction and indicator index that inflammatory bowel disease mucosa is placed in.The study found that at inflammatory bowel disease (IBD)
Calprotectin levels in the excrement of patient are apparently higher than colorectal cancer patients and irritable bowel syndrome (IBS) patient, and with trouble
Person's lesion degree is positively correlated.
In the prior art, people are by detecting the calprotectin in excrement, so that the state of an illness to patient is made
Judgement, in the prior art, people are when detecting calprotectin, it is necessary first to be sampled and carry out certain to excrement
The dilution of amount, takes sample to be added dropwise in detection kit after dilution, so that calprotectin is detected, it is still, existing
Detection kit when instilling sample sample it is possible that slide either overflow the phenomenon that, if in addition, staff goes out
Now shake can shake out the sample on kit, influence very much the working environment of staff, and traditional calprotectin inspection
Test agent box is in the inside that sample is added dropwise to kit, it is also desirable to be directed at sample hole operation, very concentration is needed to avoid sample
It scatters, it is time-consuming and laborious.
Summary of the invention
The purpose of the present invention is to provide a kind of calprotectin detection kit and its test methods, solve conventional reagents box
During use, the inconvenient of appearance is easy to appear the case where spilling leakage.
In order to achieve the above object, the present invention is achieved by the following technical programs: a kind of calprotectin detection kit,
Including shell, sample pad, colored particles/fluorescent particle conjugate pad, nitrocellulose filter are fixed in the inner cavity of the shell
And water absorption pad, the front of the shell simultaneously offer sample well right above sample pad, the front and position of the shell
Offer watch window right above nitrocellulose filter, the inside of the shell is additionally provided with transparent partition, it is described every
The inner cavity of shell is divided into two not connected spaces by plate, the sample pad, colored particles/fluorescent particle conjugate pad,
Nitrocellulose filter, water absorption pad are located at the two sides of partition with watch window and sample well respectively, are provided through on the partition
Feeding pipe is fixedly connected between the feeding pipe and partition, and the entrance of partition is connected to sample well.
The inside of the sample well is plugged with dropper, and it is flexible rubber that the bottom of the dropper, which is at the top of hard material,
Glue material composition, and through-hole is offered in dropper bottom end, the bottom end of the dropper inner cavity is fixedly installed with casing, described sleeve pipe
Bottom end is connected to through-hole, and the inner sealing of dropper is provided with baffle, and baffle is run through on the top of described sleeve pipe, described sleeve pipe
Inner tube is inlaid on inner wall, described sleeve pipe is interior and is provided with fixed plate above inner tube, and the top of said inner tube is sealed
Plug blocking, and slide bar is fixedly installed at the top of sealing-plug, the top of the slide bar is through fixed plate and sliding between fixed plate
Dynamic connection, and setting has elastic component between sealing-plug and fixed plate；
The inside of the feeding pipe is fixed with inserted link, and the radius of the inserted link is less than the radius of inner tube, and the side wall of inserted link
On be fixed with connecting rod, be fixedly connected between the other end of the connecting rod and the inner wall of feeding pipe.
Further, the sealing-plug is in inverted round table-like, and the maximum radius of sealing-plug is greater than the radius of inner tube simultaneously
It is less than the internal diameter of casing simultaneously.
Further, it is provided with hemispheric bulge-structure on the inner wall of the feeding pipe, is set on the side wall of the dropper
It is equipped with the compatible groove of bulge-structure, the bulge-structure has elasticity using rubber material production.
Further, the elastic element be spring and be wrapped on slide bar, and the both ends of spring respectively with sealing-plug with
The opposite face contact of fixed plate.
Further, it is provided with detection line and nature controlling line on the nitrocellulose filter, wherein detection line is by calprotectin
Monoclonal antibody B is sprayed, and nature controlling line is sprayed by sheep anti-mouse igg.
Further, holding part, the recess that the two sides of holding part are arc-shaped are provided on the shell.
Further, the application method of a kind of calprotectin detection kit, which is characterized in that including following step
It is rapid:
S1: sampling after being sampled with sampling rod, is put into test tube and carries out a certain amount of dilution.
S2: acquisition is acquired sample diluted in test tube using dropper
S3: dropper is inserted into the sample well on shell, fluid sample can be flowed into the interior of shell after such insertion by test
Portion simultaneously drops onto sample pad.
S4: the variation of nitrocellulose filter is observed by observation panel, to judge the content of calprotectin.
Further, in the S2 acquisition step, acquisition sample diluting liquid is 100ul.
Further, reaction in 4-6 minutes is waited after sample is added dropwise in sample pad in the S3 step.
Compare the prior art, and feeding pipe is arranged by setting dropper, and in the inside of shell in the present invention, and compare biography
The kit of system, during sample is added dropwise to kit inside, user does not have to worry sample water clock, will not go out
Existing sample is not added dropwise to the appearance of the situation inside kit accurately.
In the present invention, by improving to dropper, sample liquid is pressed by dropper by external pressure
Inside, after liquid enters in dropper, by the cooperation of sealing-plug and inner tube, so that, enter the liquid in dropper
Body will not easily be spilt, and during dropper is inserted into enclosure interior, be jacked up sealing-plug under the cooperation of inserted link,
To make sample liquid flow out, and the inside of shell is entered by feeding pipe, effectively sample drop can be avoided to come out in this way
The problem of.
In the present invention, by the cooperation of setting bulge-structure and groove, can increase between dropper and feeding pipe
The phenomenon that leakproofness effectively avoids during instilling sample liquid, the leakage of appearance.
Detailed description of the invention
Fig. 1 is schematic structural view of the invention；
Fig. 2 is structure of the invention sectional view；
Fig. 3 is dropper cross-sectional view of the structure of the present invention；
Fig. 4 is feeding pipe cross-sectional view of the structure of the present invention；
Fig. 5 is the schematic enlarged-scale view in the portion A in Fig. 3 of the present invention.
Wherein, 1 shell, 2 sample pads, 3 colored particles/fluorescent particle conjugate pad, 4 nitrocellulose filters, 5 water absorption pads,
6 sample wells, 7 watch windows, 8 partitions, 9 droppers, 10 feeding pipes, 11 holding parts, 12 through-holes, 13 casings, 14 baffles, 15 inner tubes,
16 fixed plates, 17 sealing-plugs, 18 slide bars, 19 elastic elements, 20 inserted links, 21 connecting rods, 22 bulge-structures, 23 grooves.
Specific embodiment
As shown in Figs. 1-5, the embodiment of the present invention provides a kind of calprotectin detection kit, including shell 1, the shell
Sample pad 2, colored particles/fluorescent particle conjugate pad 3, nitrocellulose filter 4 and water absorption pad 5 are fixed in 1 inner cavity,
The front of the shell 1 and the surface for being located at sample pad 2 offer sample well 6, and the front of the shell 1 is simultaneously located at nitric acid fibre
The surface for tieing up plain film 4 offers watch window 7, and the inside of the shell 1 is additionally provided with transparent partition 8, and the partition 8 will
The inner cavity of shell 1 is divided into two not connected spaces, the sample pad 2, colored particles/fluorescent particle conjugate pad 3, nitre
Acid cellulose film 4, water absorption pad 5 are located at the two sides of partition 8 with watch window 7 and sample well 6 respectively, run through on the partition 8 and set
It is equipped with feeding pipe 10, is fixedly connected between the feeding pipe 10 and partition 8, and the entrance of partition 8 is connected to sample well 6.
The inside of the sample well 6 is plugged with dropper 9, and the bottom of the dropper 9 is at the top of hard material for elasticity
Rubber material composition, and offer through-hole 12 in 9 bottom end of dropper, the bottom end of 9 inner cavity of dropper is fixedly installed with casing
13, the bottom end of described sleeve pipe 13 is connected to through-hole 12, and the inner sealing of dropper 9 is provided with baffle 14, described sleeve pipe 13
Baffle 14 is run through on top, inner tube 15 is inlaid on the inner wall of described sleeve pipe 13, described sleeve pipe 13 is interior and is located at the top of inner tube 15
It is provided with fixed plate 16, the top of said inner tube 15 is blocked by sealing-plug 17, and the top of sealing-plug 17 is fixedly installed with slide bar
18, the top of the slide bar 18 is slidably connected through fixed plate 16 and between fixed plate 16, and sealing-plug 17 and fixed plate 16
Between setting have elastic component 19；
The inside of the feeding pipe 10 is fixed with inserted link 20, and the radius of the inserted link 20 is less than the radius of inner tube 15, and inserts
It is fixed with connecting rod 21 on the side wall of bar 20, is fixedly connected between the other end and the inner wall of feeding pipe 10 of the connecting rod 21.
Sealing-plug 17 is in inverted round table-like, and the maximum radius of sealing-plug 17 is greater than the radius of inner tube 16 and is less than simultaneously
The internal diameter of casing 13.
It is provided with hemispheric bulge-structure 22 on the inner wall of feeding pipe 10, is provided with protrusion on the side wall of the dropper 9
The compatible groove 23 of structure 22, the bulge-structure 22 have elasticity using rubber material production.
Elastic element 24 be spring and be wrapped on slide bar 18, and the both ends of spring respectively with sealing-plug 17 and fixed plate 16
Opposite face contact.
Detection line and nature controlling line are provided on nitrocellulose filter 4, wherein detection line is sprayed by calprotectin monoclonal antibody B
It applies, nature controlling line is sprayed by sheep anti-mouse igg.
Holding part 11, the recess that the two sides of holding part 11 are arc-shaped are provided on shell 1.
S1: sampling after being sampled with sampling rod, is put into test tube and carries out a certain amount of dilution.
S2: acquisition is acquired sample diluted in test tube using dropper 9, and acquisition sample diluting liquid is 100ul.
S3: dropper 9 is inserted into the sample well 6 on shell 1, fluid sample can be flowed into shell 1 after such insertion by test
Inside and drop onto sample pad 2.
S4: observing the variation of nitrocellulose filter 4 by observation panel, to judge the content of calprotectin, by sample
Reaction in 4-6 minutes is waited after being added dropwise in sample pad 2.
When acquiring sample liquid, the inside of syringe is sucked the sample into injection, will be filled with sample liquid
Dropper 9 be inserted into the sample well 6 of shell 1, during insertion, inserted link 20 can across 9 bottom of dropper through-hole 12 into
Enter to the inside of casing 13, and during insertion, inserted link 20 enters the inside of inner tube 15 and connects with sealing-plug 17
Touching, sealing-plug 17 is jacked up, after sealing-plug 17 is jacked up, sample liquid will be flowed into sample pad 2, and with sample pad
It successively flows to, on colored particles/fluorescent particle conjugate pad 3 and nitrocellulose filter 4, staff, passes through observation nitric acid limit
Label on the plain film 4 in position, to judge the content of calprotectin.
Claims (9)
1. a kind of calprotectin detection kit, including shell (1), it is characterised in that: be fixed in the inner cavity of the shell (1)
Sample pad (2), colored particles/fluorescent particle conjugate pad (3), nitrocellulose filter (4) and water absorption pad (5), the shell
(1) front and being located at right above sample pad (2) offers sample well (6), and it is fine that the front of the shell (1) is simultaneously located at nitric acid
It ties up and is offered watch window (7) right above plain film (4), the inside of the shell (1) is additionally provided with transparent partition (8), institute
It states partition (8) and the inner cavity of shell (1) is divided into two not connected spaces, the sample pad (2), colored particles/fluorescence
Particle-bound pad (3), nitrocellulose filter (4), water absorption pad (5) are located at partition with watch window (7) and sample well (6) respectively
(8) two sides are provided through feeding pipe (10) on the partition (8), fixed between the feeding pipe (10) and partition (8) to connect
It connects, and the entrance of partition (8) is connected to sample well (6)；
The inside of the sample well (6) is plugged with dropper (9), and the bottom of the dropper (9) is at the top of hard material for bullet
Property rubber material composition, and offer through-hole (12) in dropper (9) bottom end, the bottom end of dropper (9) inner cavity is fixedly mounted
Have casing (13), the bottom end of described sleeve pipe (13) is connected to through-hole (12), and the inner sealing of dropper (9) is provided with baffle
(14), baffle (14) are run through on the top of described sleeve pipe (13), are inlaid with inner tube (15), the set on the inner wall of described sleeve pipe (13)
It is provided with fixed plate (16) in pipe (13) and above inner tube (15), the top of said inner tube (15) is stifled by sealing-plug (17)
Plug, and is fixedly installed with slide bar (18) at the top of sealing-plug (17), the top of the slide bar (18) through fixed plate (16) and with
Fixed plate is slidably connected between (16), and have elastic component (19) are arranged between sealing-plug (17) and fixed plate (16)；
The inside of the feeding pipe (10) is fixed with inserted link (20), and the radius of the inserted link (20) is less than the radius of inner tube (15),
And connecting rod (21) are fixed on the side wall of inserted link (20), it is solid between the other end of the connecting rod (21) and the inner wall of feeding pipe (10)
Fixed connection.
2. a kind of calprotectin detection kit according to claim 1, it is characterised in that: the sealing-plug (17) is in
That sets is round table-like, and the maximum radius of sealing-plug (17) radius for being greater than inner tube (16) and the internal diameter for being less than casing (13) simultaneously.
3. a kind of calprotectin detection kit according to claim 1, it is characterised in that: the feeding pipe (10) it is interior
It is provided on wall hemispheric bulge-structure (22), it is compatible to be provided with bulge-structure (22) on the side wall of the dropper (9)
Groove (23), the bulge-structure (22) have elasticity using rubber material production.
4. a kind of calprotectin detection kit according to claim 1, it is characterised in that: the elastic element (24) is
Spring is simultaneously wrapped on slide bar (18), and the both ends of the spring opposite face contact with sealing-plug (17) and fixed plate (16) respectively.
5. a kind of calprotectin detection kit according to claim 1, it is characterised in that: the nitrocellulose filter
(4) detection line and nature controlling line are provided on, wherein detection line is sprayed by calprotectin monoclonal antibody B, nature controlling line be by
Sheep anti-mouse igg sprays.
6. a kind of calprotectin detection kit according to claim 1, it is characterised in that: be arranged on the shell (1)
Have holding part (11), the recess that the two sides of holding part (11) are arc-shaped.
7. a kind of application method of calprotectin detection kit, characterized in that it comprises the following steps:
S1: sampling after being sampled with sampling rod, is put into test tube and carries out a certain amount of dilution.
S2: acquisition is acquired sample diluted in test tube using dropper (9)
S3: dropper (9) is inserted into the sample well (6) on shell (1) Nei, fluid sample can be flowed into shell after such insertion by test
The inside of body (1) simultaneously drops on sample pad (2).
S4: by the variation of observation panel observation nitrocellulose filter (4), to judge the content of calprotectin.
8. a kind of application method of calprotectin detection kit according to claim 7, it is characterised in that: in the S2
In acquisition step, acquisition sample diluting liquid is 100ul.
9. a kind of application method of calprotectin detection kit according to claim 7, it is characterised in that: in the S3
Reaction in 4-6 minutes is waited in step after being added dropwise to sample on sample pad (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910131322.2A CN109813917A (en) | 2019-02-22 | 2019-02-22 | A kind of calprotectin detection kit and its test method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910131322.2A CN109813917A (en) | 2019-02-22 | 2019-02-22 | A kind of calprotectin detection kit and its test method |
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| Publication Number | Publication Date |
|---|---|
| CN109813917A true CN109813917A (en) | 2019-05-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910131322.2A Pending CN109813917A (en) | 2019-02-22 | 2019-02-22 | A kind of calprotectin detection kit and its test method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110133264A (en) * | 2019-06-11 | 2019-08-16 | 上海雄图生物科技有限公司 | A kind of time-resolved fluoroimmunoassay chromatography kit detecting Mycotoxins in Feed |
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Application publication date: 20190528 |