CN109797189A - A kind of identification of target cell and killing method - Google Patents

Abstract

The invention discloses a kind of identifications of target cell and killing method, comprising: cultivates the NK cell with high expression NKg2d；Cultivate the CTL cell with high activity；NK cell with high expression NKg2d and the CTL cell with high activity are injected into abnormal target cell sample, under its microenvironment, the part NKg2d of NK cell secretion is entrenched in CTL cell surface of the same race, restore the identification and killing ability of CTL cell, CTL cell is realized to the target cell identification of the MHC-1 class molecule positive, killing and is removed, the restricted feature of non-MHC-1 for applying NK cell simultaneously, is identified, killed and is removed to the target cell of non-MHC-1 class molecule feminine gender.

Description

A kind of identification of target cell and killing method

Technical field

The present invention relates to cell field more particularly to a kind of identifications of target cell and killing method.

Background technique

There are in the sample of abnormal target cell, the identification receptor NKg2d of CTL cell surface, which is easy to fall off, causes it to exception The recognition performance of target cell declines, it is difficult to kill the abnormal target cell of low expression MHC-1 class molecule.

Summary of the invention

For overcome the deficiencies in the prior art, the purpose of the present invention is to provide a kind of identifications of target cell and killing side Method, to identify and kill abnormal target cell.

The purpose of the present invention adopts the following technical scheme that realization:

A kind of identification of target cell and killing method, comprising:

Cultivate the NK cell with high expression NKg2d；

Cultivate the CTL cell with high activity；

NK cell with high expression NKg2d and the CTL cell with high activity are injected into abnormal target cell sample, so that Part NKg2d is adhered to the CTL cell.

Further, it cultivates before there is the high NK cell for expressing NKg2d further include:

NK cell sample and CTL cell sample are extracted from sample.

Further, after extracting NK cell sample and CTL cell sample in sample further include:

The NK cell sample and the CTL cell sample are separated, cultivated and expanded.

Compared with prior art, the beneficial effects of the present invention are: by the NK cell with high expression NKg2d and have high living Property CTL cell inject abnormal target cell sample so that part NKg2d is adhered to CTL cell, to make the release of CTL cell Granzyme, lysosome, perforin, Fas, IFN etc., realize CTL cell to the identification of the target cell of MHC-1 class molecule positive, kill and It removes, while the restricted feature of non-MHC-1 of application NK cell, the target cell of non-MHC-1 class molecule feminine gender is identified, is killed Wound and removing.

Detailed description of the invention

Fig. 1 is the identification and killing method of target cell provided in an embodiment of the present invention.

Specific embodiment

In the following, being described further in conjunction with attached drawing and specific embodiment to the present invention, it should be noted that not Under the premise of conflicting, new implementation can be formed between various embodiments described below or between each technical characteristic in any combination Example.

As shown in Figure 1, the identification of target cell provided in an embodiment of the present invention and killing method, comprising:

Step S101: the NK cell with high expression NKg2d is cultivated.

Preferably, extracting NK cell sample and CTL cell sample from sample, wherein sample before cultivating NK cell Same main body is come from target cell, NK cell normal expression NKg2d, the CTL cell surface in sample has NKg2d.Extract NK After cell sample and CTL cell sample, NK cell sample and the CTL cell sample are separated, cultivated and expanded, is passed through Existing breeding method cultivates the NK cell of high expression NKg2d.

Step S102: the CTL cell with high activity is cultivated.

Specifically, cultivating the CTL cell of high activity so that CTL cell is effectively combined with NKg2d.

Step S103: the NK cell with high expression NKg2d and the CTL cell with high activity are injected into abnormal target cell Sample, so that part NKg2d is adhered to the CTL cell.

Specifically, abnormal target cell is easily parasitic or is gathered in solid tissue or organ, the CTL in abnormal target cell sample is thin The decline of born of the same parents' performance, the NKg2d on surface fall off, and cannot kill mutant.By the NK cell with high expression NKg2d and there is height Active CTL cell successively injects abnormal target cell sample, in abnormal target cell sample environment, the part of NK cell secretion NKg2d adaptively carries or is embedded in automatically CTL cell surface of the same race, to improve CTL cell to the identification energy of abnormal target cell Power, while granzyme is discharged, lysosome, perforin, Fas, IFN etc., using the targeting of CTL cell to MHC-1 class molecule sun Property target cell identification, killing and remove；The restricted feature of non-MHC-1 for utilizing NK cell simultaneously, to non-MHC-1 class molecule yin Property target cell identified, killed and removed, to the identification of the target cell of different type and property, killing and remove.

The identification and killing method of target cell provided by the invention by the NK cell with high expression NKg2d and have height Active CTL cell injects abnormal target cell sample, so that part NKg2d is adhered to CTL cell, restores the identification of CTL cell With killing ability, to make CTL cell released both particle enzyme, lysosome, perforin, Fas, IFN etc. realize CTL cell to MHC-1 Target cell identification, killing and the removing of the class molecule positive, while the restricted feature of non-MHC-1 of application NK cell, to non-MHC-1 The target cell of class molecule feminine gender is identified, killed and is removed.

The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto, The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention Claimed range.

Claims (3)

1. a kind of identification of target cell and killing method characterized by comprising

Cultivate the NK cell with high expression NKg2d；

Cultivate the CTL cell with high activity；

NK cell with high expression NKg2d and the CTL cell with high activity are injected into abnormal target cell sample, so that part NKg2d is adhered to the CTL cell surface.

2. the identification of target cell according to claim 1 and killing method, which is characterized in that cultivating has high expression Before the NK cell of NKg2d further include:

NK cell sample and CTL cell sample are extracted from sample.

3. the identification of target cell according to claim 2 and killing method, which is characterized in that it is thin to isolate NK from sample After born of the same parents' sample and CTL cell sample further include:

The NK cell sample and the CTL cell sample are separated, cultivated and expanded.