CN109655532A - A kind of method of pair of cigarette taxonomic history - Google Patents
A kind of method of pair of cigarette taxonomic history Download PDFInfo
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Abstract
The invention discloses the methods of a kind of pair of cigarette taxonomic history, carry out HS GC-Ion transfer spectrum analysis to cigarette sample, obtain its three-dimensional spectrum；The selected characteristic analyzed area in collected cigarette sample three-dimensional spectrum；The signature analysis region of the three-dimensional spectrum of collected cigarette sample is analyzed, taxonomic history is carried out to cigarette.Head space-gas phase-ion mobility spectrometry joint technology is used to cigarette Fast Classification to identify, method resolution ratio and high sensitivity, analysis time are short, and lossless, no sample pre-treatment, method is environmental-friendly, and it is few to test consumptive material used, testing cost is low, visual result, is suitble to cigarette sample batch taxonomic history.
Description
Technical field
The present invention relates to quick analysis detection fields, more particularly to the method for a kind of pair of cigarette taxonomic history.
Background technique
Ion mobility spectrometry (IMS) is a kind of trace chemical analysis and detection technology that fast development is got up in recent years, it is
Based on the difference of gaseous ion different in gas phase migration velocity in the electric field come characterized to chemical ion substance one
Analytical technology.Under normal pressure, test substance passes through charge on ionization source band, substance drift under the action of voltage, due to ion
Charge, cross-sectional area, quality etc. are not exactly the same, therefore the drift time in drift pipe is different, thus reach detector
Time is different, according to product ion in the drift time of drift pipe, detects to determinand.IMS detection sensitivity is high, responds
Speed is fast, and advantage is extremely prominent in the trace detection of volatile organic compounds, and detection limit can be even pg grades down to ng, is suitble to
Field quick detection or analysis, detection time are often Millisecond.But IMS also has disadvantage, i.e., only carries out by drift pipe to compound
Separation, inferior separating effect, and gas-chromatography and IMS are combined, and GC separation characteristic outstanding and IMS quick response, Gao Ling is utilized
The advantage of sensitivity, efficiently solves the low distinguishing ability of GC and when IMS detects mixture existing cross sensitivity is asked
Topic, finally obtains the three-dimensional spectrum of retention time, drift time and signal strength, keeps qualitative analysis more accurate.And it is equipped with
The fast gas chromatography disengaging time of multiple capillary chromatographic column is substantially reduced compared with conventional chromatogram, can satisfy live quick point
The needs of analysis.
For different trade mark cigarette because of tobacco leaf formulation difference, the flavors and fragrances of addition is different, makes it have unique fragrance and waves
Hair property ingredient, that is, be different from the chemical composition of other cigarette, and this results in their respective fingerprint characteristics and brand identities.Sense organ
Evaluation is the intuitive judgment of cigarette style characteristic, but sensory quality assessment method itself has biggish subjectivity, vulnerable to smokeing panel test
The influence of the factors such as person's mood, physical condition.
Therefore it provides different trade mark cigarette can be carried out accurate Fast Classification by the method for a kind of pair of cigarette taxonomic history,
It is a technical problem that technical personnel in the field need to solve at present without sample pre-treatments, visual result.
Summary of the invention
The object of the present invention is to provide the methods of a kind of pair of cigarette taxonomic history, and it is accurate to carry out to different trade mark cigarette
The identification of Fast Classification, without complicated sample pre-treatments, visual result.
In order to solve the above technical problems, the present invention provides the following technical scheme that
The method of a kind of pair of cigarette taxonomic history, comprising: HS GC-ion mobility spectrometry is carried out to cigarette sample
Analysis, obtains its three-dimensional spectrum；The selected characteristic analyzed area in the three-dimensional spectrum of collected cigarette sample；To collected
The signature analysis region of the three-dimensional spectrum of cigarette sample is analyzed, and is identified to cigarette.
Preferably, HS GC-Ion transfer spectrum analysis is carried out to cigarette sample, obtains its three-dimensional spectrum, wrapped
It includes: in gas-chromatography-Ion mobility spectrometry, using the head space-of the model FlavourSpec of German G.A.S instrument company
Gas-chromatography-ion mobility spectrometry equipment；
Set gas-chromatography-Ion mobility spectrometry condition: chromatographic column is multiple capillary splitter, stationary phase OV-5,
32 DEG C of chromatogram column temperature；80 DEG C of injector temperature；Carrier gas is high pure nitrogen, and flow rate of carrier gas rises gas mode, initial flow using program
Fast 8ml/min rises to 50ml/min after retaining 40s when 4min, and when 5min rises to 150ml/min, and 150ml/min is protected always
It holds to 8min, flow velocity is down to initial flow 8ml/min when 8.5min；Migration spectrum drift gas flow 200ml/min；Migration spectrum drift
Move pipe temperature 45 C.
Preferably, HS GC-Ion transfer spectrum analysis is carried out to cigarette sample, obtains its three-dimensional spectrum, wrapped
Include: set head space condition: incubation temperature is 80 DEG C；Brooding time is 5min；Sampling volume is 200 μ l；Sample introduction needle temperature is 80
℃。
Preferably, the three-dimensional spectrum selected characteristic analyzed area of collected cigarette sample is analyzed, to cigarette into
Row identifies, comprising: the view in the collected cigarette sample three-dimensional spectrum signature analysis region is generated by Gallery plug-in unit
Library, observation compare the appearance situation of each analyzed area signal peak, identify to cigarette；If the appearance phenomenon difference is obvious not
Together, then determine the cigarette for different trade mark cigarette samples.
Preferably, the three-dimensional spectrum selected characteristic analyzed area of collected cigarette sample is analyzed, to cigarette into
Row identifies, comprising: the knot in the signature analysis region of the three-dimensional spectrum of collected cigarette sample is generated using Gallery plug-in unit
Fruit obtains the whole matching angle value of selected analyzed area between each cigarette sample；It will matching angle value and the progress of preset matching degree threshold value
Comparison；If matching angle value is less than the matching degree threshold value, determine cigarette for different trade mark cigarette samples；If matching angle value is greater than
Preset matching degree threshold value then determines that cigarette is identical trade mark cigarette sample.
Preferably, the signature analysis region of the three-dimensional spectrum of collected cigarette sample is analyzed, cigarette is carried out
Identify, comprising: can by chemometrics method progress to the signature analysis region of the three-dimensional spectrum of collected cigarette sample
It is analyzed depending on changing, cigarette is identified.
Preferably, chemometrics method carries out visual analyzing, comprising: principal component analysis, factorial analysis differentiate and divide
Analysis, partial least squares discriminant analysis.
Preferably, chromatographic column further include: using the multi-capillary column or single long capillary column of other stationary phases.
Compared with prior art, above-mentioned technical proposal has the advantage that
The method of a kind of pair of cigarette taxonomic history provided by the present invention, to cigarette sample carry out HS GC-from
Son migration spectrum analysis, obtains its three-dimensional spectrum；The selected characteristic analyzed area in the three-dimensional spectrum of collected cigarette sample；It is right
The signature analysis region of the three-dimensional spectrum of collected cigarette sample is analyzed, and is identified to cigarette.By head space-gas phase-
Ion mobility spectrometry joint technology identifies for cigarette Fast Classification, and method resolution ratio and high sensitivity, analysis time is short, and nothing
Damage, no sample pre-treatment, method is environmental-friendly, tests that consumptive material used is few, and testing cost is low, visual result, is suitble to cigarette sample
Product batch taxonomic history.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is the present invention
Some embodiments for those of ordinary skill in the art without creative efforts, can also basis
These attached drawings obtain other attached drawings.
Fig. 1 is the method flow diagram of a kind of pair of cigarette taxonomic history provided by a kind of specific embodiment of the present invention；
Fig. 2 is headspace gas chromatography-ion mobility spectrometry (GC-IMS) structural schematic diagram；
Fig. 3 is tri- trade mark cigarette GC-IMS three-dimensional spectrums of A, B, C；
Fig. 4 is that the selected signature analysis region of the trade mark cigarette 21 of A, B, C tri- regards library figure；
Fig. 5 is that A, B cigarette different batches select analyzed area matching degree comparison histogram；
Fig. 6 is that B, C cigarette sample select analyzed area matching degree comparison histogram；
Fig. 7 is OPLS-DA model the 1st, the 2 principal component scores figures in D, E cigarette sample signature analysis region；
Fig. 8 is the S-Plot figure that cigarette OPLS-DA analyzes 18 variables.
Specific embodiment
Core of the invention is to provide the method for a kind of pair of cigarette taxonomic history, and it is accurate to carry out to different trade mark cigarette
Fast Classification, without complicated sample pre-treatments, visual result.
It is understandable in order to enable the above objects, features and advantages of the present invention to become apparent, with reference to the accompanying drawing to this hair
Bright specific embodiment is described in detail.
Detail is elaborated in the following description to fully understand the present invention.But the present invention can with it is a variety of not
Other way described herein is same as to implement, those skilled in the art can do class without violating the connotation of the present invention
Like popularization.Therefore the present invention is not limited to the specific embodiments disclosed below.
Referring to FIG. 1, Fig. 1 is the method for a kind of pair of cigarette taxonomic history provided by a kind of specific embodiment of the present invention
Flow chart.
A kind of specific embodiment of the invention provides the method for a kind of pair of cigarette taxonomic history, comprising steps of
S11: HS GC-Ion transfer spectrum analysis is carried out to cigarette sample, obtains its three-dimensional spectrum.
According to Headspace-Gas Chromatography Analysis-ion mobility spectrometry (HS-GC-IMS) method, different cigarette samples is divided
Analysis, finally obtains the spectrogram of analysis.
S12: in the three-dimensional spectrum selected characteristic analyzed area of collected cigarette sample.
According to the three of the collected cigarette sample generated by HS GC-Ion mobility spectrometry of step S11
Spectrogram is tieed up, the signature analysis region that can characterize each cigarette sample volatility and semi-volatility component is chosen on the three-dimensional spectrum.
S13: the signature analysis region of the three-dimensional spectrum of collected cigarette sample is analyzed, is reflected to cigarette
Not.
In step s 12, signature analysis region is had chosen to the three-dimensional spectrum of collected cigarette sample, according to right
Signature analysis region is analyzed, and is identified to cigarette.
Can fast and accurately it be classified to different trade mark cigarette using this method, by directly acquiring cigarette sample to be sorted
HS-GC-IMS spectrogram compares the signature analysis region of cigarette sample by software, the data processing software carried using instrument
Gallery generate view library figure, or to analyzed area carry out whole matching degree compare, or by statistics software such as spss,
The softwares such as simca-p, matlab to signature analysis region carry out chemometrics application for example principal component analysis, discriminant analysis, partially
Least square method discriminant analysis etc., which is realized, carries out accurate Fast Classification identification to different trade mark finished cigarettes.
In a kind of embodiment provided by the present invention, HS GC-ion step S11: is carried out to cigarette sample
Spectrum analysis is migrated, its three-dimensional spectrum is obtained, may include:
In gas-chromatography-Ion mobility spectrometry, using the model FlavourSpec of German G.A.S instrument company
HS GC-ion mobility spectrometry equipment；
Set gas-chromatography-Ion mobility spectrometry condition: chromatographic column is multiple capillary splitter, stationary phase OV-5,
32 DEG C of chromatogram column temperature；80 DEG C of injector temperature；Carrier gas is high pure nitrogen, and flow rate of carrier gas rises gas mode, initial flow using program
Fast 8ml/min rises to 50ml/min after retaining 40s when 4min, and when 5min rises to 150ml/min, and 150ml/min is protected always
It holds to 8min, flow velocity is down to initial flow 8ml/min when 8.5min；Migration spectrum drift gas flow 200ml/min；Migration spectrum drift
Move pipe temperature 45 C.
Above-mentioned gas-chromatography-Ion transfer spectral condition is a kind of optimum condition of specific embodiment of the present invention, using it
His condition may also obtain preferable analysis as a result, can specifically set according to the actual situation, and the application is for the condition
Setting without limitation.
Different batches flavors and fragrances sample is analyzed using the equipment, it, can also be first to this before obtaining analysis of spectra
Gas-chromatography-ion mobility spectrometry equipment is cleaned, in case other remaining substances of the equipment have an impact analysis result.
In a kind of embodiment provided by the present invention, HS GC-ion step S11: is carried out to cigarette sample
Spectrum analysis is migrated, its three-dimensional spectrum is obtained, may include:
Setting head space condition: 80 DEG C of incubation temperature；Brooding time 5min；200 μ l of sampling volume；80 DEG C of sample introduction needle temperature.
Above-mentioned head space condition is a kind of optimum condition of specific embodiment of the present invention, may also be obtained using other conditions
Preferably analysis as a result, can specifically be set according to the actual situation, the application for the condition setting without limitation.
In a kind of embodiment provided by the present invention, step S13: the three-dimensional spectrum of collected cigarette sample is chosen
Signature analysis region is analyzed, and is identified to cigarette, may include: described collected by the generation of Gallery plug-in unit
The view library in cigarette sample three-dimensional spectrum signature analysis region, observation compares the appearance situation of each analyzed area signal peak, to volume
Cigarette is identified；If the appearance phenomenon difference is significantly different, determine the cigarette for different trade mark cigarette samples.
In a kind of embodiment provided by the present invention, step S13: the three-dimensional spectrum of collected cigarette sample is chosen
Signature analysis region is analyzed, and is identified to cigarette, may include:
Using Gallery plug-in unit generate the signature analysis region of the three-dimensional spectrum of collected cigarette sample as a result,
Whole matching angle value to analyzed area selected between each cigarette sample matches angle value；Will matching angle value and preset matching degree threshold value into
Row comparison；If matching angle value is less than the matching degree threshold value, determine cigarette for different trade mark cigarette samples；If it is big to match angle value
In preset matching degree threshold value, then determine that cigarette is identical trade mark cigarette sample.
G.A.S company provides a series of integrated software analysis tools, it can be achieved that automatic data acquisition, data visualization,
Assessment, calibration, the quantitative and functions such as qualitative, the LAV software and Gallery plug-in unit provided using G.A.S company in the present embodiment
The signature analysis region of the three-dimensional spectrum of collected cigarette sample is analyzed, and is carried out pair with preset matching degree threshold value
Than being classified with this to cigarette sample.
In a kind of embodiment provided by the present invention, step S13: the three-dimensional spectrum of collected cigarette sample is chosen
Signature analysis region is analyzed, and is identified to cigarette, may include: the spy to the three-dimensional spectrum of collected cigarette sample
It levies analyzed area and visual analyzing is carried out by chemometrics method, cigarette is identified.Chemometrics method carries out
Visual analyzing, comprising: principal component analysis, factorial analysis, discriminant analysis, partial least squares discriminant analysis.
In a kind of embodiment provided by the present invention, HS GC-ion step S11: is carried out to cigarette sample
Spectrum analysis is migrated, its three-dimensional spectrum is obtained, can also include:
Using the multi-capillary column or single long capillary column of other stationary phases；
Preferable separating effect such as can be obtained using 60m long DB-5 capillary column, but on analysis time, compared with MCC
Capillary column is slow；It according to the actual situation and can need to select suitable chromatographic column.
Preferably to help to understand, citing is illustrated above method below:
Headspace gas chromatography-ion mobility spectrometry (GC-IMS) structural schematic diagram is as shown in Figure 2；
In figure, Drift gas is drift gas, and Carrier gas is gas of carrier gas, and T1 is migration spectrum drift tube temperature,
T2 is chromatogram column temperature, and T3 is injector temperature, and IMS is ionic migration spectrometer, and EPC1 is migration spectrum drift gas flow, EPC2
For flow rate of carrier gas, sample is sample.
Embodiment one:
1) preparation of cigarette sample
The cigarette sample for choosing the different trades mark to be analyzed, taking 3 cigarette at random from each cigarette sample, (nominal cigarette is not
Choosing) it is fitted into the ml headspace bottle of 20ml, a cigarette is packed into each ml headspace bottle, gland sealing is to be measured.
2) cigarette sample is analyzed using HS-GC-IMS
Analysis condition is as follows:
Head space condition: 80 DEG C of incubation temperature；Brooding time 5min；200 μ l of sampling volume；80 DEG C of sample introduction needle temperature；
GC-IMS condition: chromatographic column be multiple capillary splitter (MCC), stationary phase OV-5,32 DEG C of chromatographic column temperature；Injection port
80 DEG C of temperature；Carrier gas is high pure nitrogen；Flow rate of carrier gas: using program rise gas mode, initial flow rate 8ml/min, retain 40s after,
Rise to 50ml/min when 4min, when 5min rises to 150ml/min, and 150ml/min is always maintained to 8min, flow velocity when 8.5min
It is down to initial flow 8ml/min.
Migration spectrum drift gas flow 200ml/min；45 DEG C of migration spectrum drift tube temperature.
3) sample analysis and spectrogram are evaluated
The volatile and semi-volatile compounds for analyzing tri- trade mark cigarette samples of A, B, C under the above conditions, obtain its HS-
GC-IMS three-dimensional spectrum.The spectrogram marker of analyzed cigarette, i.e. the signature analysis region of cigarette sample are selected, passes through what is selected
Analyzed area generates view library using the Gallery plug-in unit that software carries, and is compared, reaches pair to analyzed area appearance situation
The purpose of cigarette Fast Classification.
Fig. 3 is tri- trade mark cigarette GC-IMS three-dimensional spectrums of A, B, C.Most of peak concentrates on preceding 5min, and all peaks are basic
It is complete out in 7min.
21 analyzed areas with obvious characteristic for choosing three trade mark cigarette three-dimensional spectrums, are handled by instrument data
The included Gallery plug-in unit of software generates view library figure, as shown in figure 4, Fig. 4 is 18 batch A cigarette, 17 batch B cigarette, 15
A batch C cigarette selectes the view library of analyzed area, and different trade mark cigarette have strong response, same board in different analyzed areas
The Ion transfer spectral peak of cigarette is extremely similar between number different batches, and peak shape is not much different with peak intensity, the peak shape between the trade mark with
Peak intensity has significant difference.Analyzed area whole matching degree is 80% or more between same trade mark cigarette batch, and between the different trades mark
Analyzed area matching degree 65% hereinafter, as shown in figure 5, by the basis of A4 cigarette sample, remaining cigarette sample and its into
Row comparison, A1-A18 sample match degree is higher, matching degree > 80%, and B1-B17 cigarette sample and A1-A18 cigarette sample
Matching degree < 65% of product, similitude is poor, illustrate No. A1-A18 from No. B1-B17 be the different trades mark cigarette sample.With No. B1
On the basis of cigarette sample, remaining cigarette sample is compared with it, matching degree > 80% between B1-B17 cigarette sample, and B1-
Matching degree < 65% of B17 cigarette sample and C1-C15 cigarette sample, similitude is poor, illustrates No. B1-B17 and C1-C15
It number is the cigarette sample of the different trades mark.The method is carried out classification prediction for the blind sample of A, B, C cigarette, accuracy rate is up to 100%.
Embodiment two:
1) preparation of cigarette sample
Collecting D, E trade mark cigarette, closely different batches cigarette sample, each batch choose 3 cigarette (bear in parallel in two years
Cigarette does not select) be fitted into the ml headspace bottle of 20ml seal it is to be measured.
2) cigarette sample is analyzed using HS-GC-IMS
Analysis condition is the same as example 1.
3) sample analysis and spectrogram are evaluated
18 signature analysis regions for choosing D, E cigarette sample GC-IMS three-dimensional spectrum, using 13 software pair of simca-p
It carries out data analysis.D cigarette acquires 98 batches, and E cigarette acquires 34 batches, first carries out principal component point respectively to D and E
Analysis checks that whether there is or not abnormal points in sample, reject after having abnormal point then to search reason；Then D and E sample is carried out orthogonal partially minimum
Two multiply diagnostic method (OPLS-DA) analysis, and each analyzed area peak height is independent variable X, classified variable Y, and it is special to establish cigarette GC-IMS
The disaggregated model for levying analyzed area, the classification for be used for after inside and outside verifying to sample to model are predicted.With 90 D and
OPLS-DA disaggregated model is established as training set in the signature analysis region of E cigarette sample, and 3 principal components of extraction are to original number
According to the accumulation interpretability [R of matrix2X (CUM)] up to 86.1%, cross validation obtains total explained variance [R of classified variable2
It (CUM)] is 98.8%, the predictive ability [Q of model2(CUM)] up to 98.5%, R2(CUM) and Q2(CUM) difference is much smaller than
0.3, illustrate that this model is an outstanding classification prediction model.The OPLS-DA model score figure of Fig. 7 can intuitively show model
Classifying quality, two groups of sample separation degrees are big in figure, illustrate that the category of model effect is good.It can be found from the S-PLOTL figure of Fig. 8
The difference marker of 2 groups of sample variables, as shown in frame red in figure.Using the signature analysis region of 32 D, E cigarette samples as testing
Card collection carries out external certificate to OPLS-DA disaggregated model, determines by decision rule prediction result, as a result, it has been found that 31 samples
This obtains correct judgement.The method is suitble to identify cigarette high-volume Fast Classification.
In conclusion the method for a kind of pair of cigarette taxonomic history provided by the present invention, carries out head space-gas to cigarette sample
Phase chromatography-Ion transfer spectrum analysis, obtains its three-dimensional spectrum；The selected characteristic point in the three-dimensional spectrum of collected cigarette sample
Analyse region；The signature analysis region of the three-dimensional spectrum of collected cigarette sample is analyzed, taxonomic history is carried out to cigarette.
HS GC-ion mobility spectrometry joint technology is used to cigarette Fast Classification to identify, method resolution ratio and high sensitivity,
Analysis time is short, and lossless, no sample pre-treatment, and method is environmental-friendly, tests that consumptive material used is few, and testing cost is low, as a result
Intuitively, it is suitble to cigarette sample batch taxonomic history.
The method of a kind of pair of cigarette taxonomic history provided by the present invention is described in detail above.It is used herein
A specific example illustrates the principle and implementation of the invention, and the above embodiments are only used to help understand
Method and its core concept of the invention.It should be pointed out that for those skilled in the art, not departing from this
, can be with several improvements and modifications are made to the present invention under the premise of inventive principle, these improvement and modification also fall into the present invention
In scope of protection of the claims.
Claims (8)
1. the method for a kind of pair of cigarette taxonomic history characterized by comprising
HS GC-Ion transfer spectrum analysis is carried out to cigarette sample, obtains corresponding three-dimensional spectrum；
The selected characteristic analyzed area in collected cigarette sample three-dimensional spectrum；
The signature analysis region of the collected cigarette sample three-dimensional spectrum is analyzed, taxonomic history is carried out to cigarette.
2. the method according to claim 1, wherein carrying out HS GC-Ion transfer to cigarette sample
Spectrum analysis obtains corresponding three-dimensional spectrum, comprising:
In the HS GC-Ion mobility spectrometry, using the HS GC-of model FlavourSpec
Ion mobility spectrometry equipment；
Set gas-chromatography-Ion mobility spectrometry condition: chromatographic column is multiple capillary splitter, stationary phase OV-5, chromatography
32 DEG C of column temperature；80 DEG C of injector temperature；Carrier gas is high pure nitrogen, and flow rate of carrier gas rises gas mode, initial flow rate using program
8ml/min rises to 50ml/min after retaining 40s when 4min, and when 5min rises to 150ml/min, and 150ml/min is always maintained at
To 8min, flow velocity is down to initial flow 8ml/min when 8.5min；Migration spectrum drift gas flow 200ml/min；Migrate Frequency bias
Pipe temperature 45 C.
3. the method according to claim 1, wherein carrying out HS GC-Ion transfer to cigarette sample
Spectrum analysis obtains corresponding three-dimensional spectrum, comprising:
Setting head space condition: 80 DEG C of incubation temperature；Brooding time 5min；200 μ l of sampling volume；80 DEG C of sample introduction needle temperature.
4. method according to any one of claims 1 to 3, which is characterized in that collected cigarette sample three-dimensional spectrum
Middle selected characteristic analyzed area is analyzed, and is identified to cigarette, comprising:
The view library in the collected cigarette sample three-dimensional spectrum signature analysis region is generated by Gallery plug-in unit, is observed
The appearance situation for comparing each analyzed area signal peak, identifies cigarette；If the appearance phenomenon difference is significantly different, sentence
The fixed cigarette is different trade mark cigarette samples.
5. method according to any one of claims 1 to 3, which is characterized in that collected cigarette sample three-dimensional spectrum
Middle selected characteristic analyzed area is analyzed, and is identified to cigarette, comprising:
Using Gallery plug-in unit generate the signature analysis region of the three-dimensional spectrum of collected cigarette sample as a result, obtaining each
The whole matching angle value of selected analyzed area between cigarette sample；
The matching angle value and preset matching degree threshold value are compared；If the matching angle value is less than the preset matching degree threshold
Value, then determine the cigarette for different trade mark cigarette samples；If the matching angle value is greater than the preset matching degree threshold value, sentence
The fixed cigarette is identical trade mark cigarette sample.
6. method according to any one of claims 1 to 3, which is characterized in that three-dimensional to the collected cigarette sample
The signature analysis region of spectrogram is analyzed, and is identified to cigarette, comprising:
The signature analysis region of the three-dimensional spectrum of the collected cigarette sample is carried out visually by chemometrics method
Change analysis, cigarette is identified.
7. according to the method described in claim 6, it is characterized in that, the three-dimensional spectrum to the collected cigarette sample
Signature analysis region by chemometrics method carry out visual analyzing include:
Using principal component analysis, and/or factorial analysis, and/or discriminant analysis, and/or partial least squares discriminant analysis to described
The signature analysis region of the three-dimensional spectrum of collected cigarette sample carries out visual analyzing.
8. according to the method described in claim 2, it is characterized in that, the chromatographic column further include:
Using the multi-capillary column or single long capillary column of stationary phase.
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