CN109452292B

CN109452292B - Bactericidal composition and application thereof

Info

Publication number: CN109452292B
Application number: CN201811253157.XA
Authority: CN
Inventors: 孙莹莹; 陈熙; 李翔
Original assignee: Sichuan Lier Crop Science Co Ltd
Current assignee: Sichuan Lier Crop Science Co Ltd
Priority date: 2014-04-17
Filing date: 2014-04-17
Publication date: 2021-04-16
Anticipated expiration: 2034-04-17
Also published as: CN109287643B; CN106719711A; CN106719711B; CN105010358B; CN109287643A; CN109452292A; CN105010358A

Abstract

The invention discloses a bactericide composition and application thereof, and belongs to the field of pesticides, wherein the active ingredients of the bactericide composition comprise (A) methicillin-induced amine and (B) amide bactericides, and the weight ratio of (A) to (B) is 1: 30-40: 1, preferably 1: 15-20: 1, and more preferably 1: 7-10: 1. The amide bactericide (B) may be selected from boscalid, thifluzamide, furametpyr and the like. The composition can be added with other auxiliary components allowed to be used in pesticides and processed into any dosage form. The bactericidal composition has the effect obviously higher than that of the single agent (A) and the single agent (B), has obvious synergistic effect, effectively reduces the application amount of each single agent, has better effects of expanding bactericidal spectrum and delaying the drug resistance of plants, and can effectively prevent and treat tobacco virus diseases, black shank, early blight of tomatoes, late blight, cucumber downy mildew, pepper blight, damping off and the like.

Description

Bactericidal composition and application thereof

The present application is a divisional application with application number 201410155202.3(201611082145.6) filed on 17.04.2014, entitled "germicidal composition and use thereof".

Technical Field

The invention relates to a bactericidal composition, in particular to a bactericidal composition containing methine luring amine and an amide bactericide and application thereof.

Background

In agricultural production, whether crops can grow healthily is also important for preventing and treating diseases besides being affected by pests and weeds. With the enhancement of environmental protection concept and the implementation of sustainable development strategy, high efficiency, low toxicity, high activity and low residue have become the inevitable trend of pesticide development. However, even if the high-efficiency bactericide is used alone for a long time, the diseases are easy to generate drug resistance, the consumption is increased, the control effect is reduced, the lasting period is shortened, and the like, and the environment sustainable development is not facilitated. Therefore, how to scientifically apply the pesticide, reduce the dosage of the chemical pesticide and improve the pesticide effect becomes a problem to be solved urgently in the field of plant protection.

The acitreine (SZG) is a thiadiazole plant disease prevention activator jointly developed by Lier chemical Co-Ltd and southern Kao university, has the chemical common name of N- (5-methyl-1, 3-thiazole-2-yl) -4-methyl-1, 2, 3-thiadiazole-5-formamide, and has the structural formula shown in (I). The current research result shows that the compound has good effect of inducing disease resistance to virus diseases, and the action mechanism is inducing plants to generate systemic and broad-spectrum disease resistance activity. The plant disease prevention activator is a novel pesticide which is concerned in recent years, has no obvious bactericidal activity, but can be used as an immune activator of plants to induce a plant system to obtain resistance, thereby achieving the purposes of disease resistance and disease prevention. Among them, BTH (benzothiadiazole, manufactured by mr. switzerland) and TDL (tiadinil, manufactured by japan pesticide co., ltd.) are representative varieties of such agents, and have been widely used in agricultural production at present. But the single agent of the methine luring amine is unstable in field performance, so that the control effect generates certain fluctuation, and the single agent has no good treatment effect on diseased plants.

The amide bactericides have a very important position in the world bactericide market, are often used as special medicaments for preventing and controlling peronosporales fungi, have obvious protection, treatment and eradication effects, and are widely applied to the prevention and control of plant diseases. However, the amide bactericides have a single effect on pathogenic bacteria, and once the pathogenic bacteria mutate, the bactericide cannot normally function, so that the pathogenic bacteria are easy to generate drug resistance. Common amide bactericides on the market comprise dimethomorph, boscalid, thifluzamide, furametpyr, metalaxyl and the like.

The structural formula of the dimethomorph is shown as (II). The bactericide is a specific bactericide for killing oomycetes fungi, has the action characteristic of destroying the formation of cell wall membranes, has action on each stage of the life history of the oomycetes, is particularly sensitive in the formation stage of sporangium peduncles and oospores, and is inhibited under the condition of extremely low concentration (<0.25 mu g/ml). Alone has a higher resistance risk, so the single-component antibacterial agent is often compounded with a protective bactericide to delay the generation of resistance.

The boscalid has a structural formula shown as (III). Is a novel nicotinamide fungicide developed by basf, germany, which is mainly used for controlling powdery mildew, gray mold, various rot diseases, brown rot, root rot, and the like, and has been registered in uk, germany, and switzerland in 2004. Boscalid belongs to a succinate coenzyme Q reductase inhibitor in a mitochondrial respiratory chain, has strong inhibition capacity on the germination of spores, and has no cross resistance with other bactericides.

The structure formula of the thiaflutolamide is shown as (IV). Is a succinate dehydrogenase inhibitor, namely inhibits the synthesis of succinate dehydrogenase in the fungus tricarboxylic acid cycle. Can prevent and treat various diseases, in particular to diseases caused by basidiomycetous rhizoctonia fungi, and simultaneously has strong systemic conductivity. But has poor effect on diseases caused by leaf pathogens, such as peanut brown spot and black spot.

Furametpyr has a structural formula shown as (V). Is a novel amide fungicide developed by Sumitomo chemical industries, Japan. Since 1989, intensive studies on the prevention and control of sheath blight have been conducted in laboratories and in the fields, and the results show that: the control effect of preventing and treating the rice sheath blight is excellent by applying wettable powder, powder on leaf surfaces or applying granules in a flooding way. However, due to long-term use, crops have already generated serious drug resistance to the pesticide, so that the pesticide duration is shortened, the pesticide application amount is increased, and the control effect is reduced.

Metalaxyl, the structural formula of which is shown as (VI). Is the first bactericide with stereoactive activity on the market and can be used for seed treatment, soil treatment and stem leaf treatment. It is a systemic bactericide with protection and treatment effects, can be absorbed by plant roots, stems and leaves, and transferred to plant organs along with the transportation of water in plant body. However, due to long-term use, crops have already generated serious drug resistance to the pesticide, so that the pesticide duration is shortened, the pesticide application amount is increased, and the control effect is reduced.

The bactericide containing a single active component is frequently used, so that most of germs generate drug resistance, and the control effect is not ideal. Therefore, a novel product is urgently needed to replace a sterilization product with a single active component, increase the drug effect, delay the drug resistance risk of diseases and reduce the economic loss.

Disclosure of Invention

In view of the above situation, the present invention provides a bactericidal composition to meet the needs of agricultural production, and the active ingredients contained in the composition have synergistic effect with each other to improve the control effect and to delay the generation of drug resistance of germs.

In order to solve the technical problems, the invention provides the following technical scheme: the bactericidal composition comprises the effective components of methyl thiazole induced amine and amide bactericides, wherein the weight ratio of the methyl thiazole induced amine to the amide bactericides is 1: 30-40: 1, preferably in a weight ratio of 1: 15-20: 1, more preferably in a weight ratio of 1: 7-10: 1.

the amide fungicide can be any one of fluopicolide, pyridylamine, ethaboxam, cyflufenamid, thifluzamide, zoxamide, fenhexamid, silthiopham, dimethomorph, boscalid, thifluzamide, furametpyr or metalaxyl. According to the experimental effect, the dimethomorph, the boscalid, the thifluzamide, the furametpyr or the metalaxyl are preferably compounded.

The bactericidal composition can also contain auxiliary components of pesticide preparations so as to prepare formulations suitable for agriculture.

The composition of the present invention may further comprise auxiliary agents including wetting agents, dispersants, emulsifiers, solvents, fillers, and other known substances beneficial to the stabilization and efficacy exertion of active ingredients in the preparation, which are various ingredients commonly used or allowed to be used in pesticide preparations, and are not particularly limited, and the ingredients and the amount of the ingredients are determined by simple tests according to the formulation requirements.

The bactericidal composition can be prepared into any one dosage form suitable for agriculture by using a known method, and the better dosage forms are Wettable Powder (WP), Water Dispersible Granules (WDG), suspending agents (SC), dry suspending agents (DF) and the like.

B. Wettable Powders (WP), which are solid powder formulations uniformly dispersible in water. The wettable powder of the invention is added with diluents or inert substances such as white carbon black, light calcium carbonate, argil, diatomite, attapulgite and the like and ionic or nonionic surfactants (wetting agents and dispersing agents), wherein the wetting agents include alkylbenzene sulfonate (DBS-Na), alkyl naphthalene sulfonate (nekal), alkylphenol polyoxyethylene ether sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, fatty alcohol ethylene oxide adduct sulfonate, alkylamide taurate and fatty alcohol polyoxyethylene ether (JFC), and the content is 0.1-30%; dispersants such as naphthalene sulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonate (NO, NN0, MF), lignin and its derivative sulfonate (M-9, POLYFON, etc.), polyoxyethylene polyoxypropylene ether block copolymer, alkylphenol polyoxyethylene ether formaldehyde condensate Sulfate (SOPA), PVA, CMC content 0.1-30%; also contains one or more substances of penetrant JFC, azone, thiazone and penetrant T, and the content is 0.3-30%; other additives such as stabilizer, foam inhibitor, anti-caking agent, etc. can also be contained.

C. Water Dispersible Granule (WDG) is a granule which can disintegrate and disperse in water quickly to form a highly suspended dispersion system. Consists of active ingredients, auxiliary agents such as wetting agents, dispersing agents, isolating agents, disintegrating agents, stabilizing agents, bonding agents and the like, carriers and other elements. The formulation of the adjuvants and carriers, and the precursors (pre-forms before granulation) are essentially similar to the wettable powders and suspending agents.

D. A Dry Flowable (DF) is a granule which can be disintegrated and dispersed in water quickly to form a high-suspension dispersion system. The preparation method comprises the following steps: the formulation resulting from the dehydration of the suspending agent. The formulation of the adjuvants and carriers, and the precursors (preformed objects before dehydration and drying) are in accordance with the suspending agents.

E. Suspending agents (SC) are dispersions of water-insoluble solid pesticides or immiscible liquid pesticides in water or oil. Is prepared by wet superfine grinding of active ingredients and auxiliary agents (wetting dispersant, thickening agent, stabilizing agent, pH regulator, defoaming agent and antifreezing agent). The wetting dispersant can be one or more of naphthalene or alkyl naphthalene formaldehyde condensate sulfonate (NO, NN0, MF), lignin and derivative sulfonate (M-9, POLYFON and the like), alkylphenol polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene ether block copolymer, alkylphenol polyoxyethylene phosphate, carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), condensed phosphate and the like, and the content is 0.1-12%; the thickening agent and the stabilizing agent can be one or more of Xanthan Gum (XG), polyvinyl alcohol (PVA), aluminum magnesium silicate and the like, and the content is 0.05-0.5%; the pH regulator and the defoaming agent can be added in proper amount according to the situation.

The products described herein may be provided in a finished formulation, i.e. the compounds are mixed already, or the components of the composition may be provided in a single dose, mixed directly in a tank or tank prior to use, and then diluted to the desired concentration.

The bactericidal composition can be used for preventing and treating various plant diseases, and is particularly suitable for preventing and treating plant diseases such as tobacco virus diseases, black shank diseases, tomato early blight, late blight, cucumber downy mildew, pepper phytophthora blight, damping off, peanut brown spot, black spot and the like. The compositions of the invention can be applied by conventional means, such as pouring, spraying, misting, dusting or sprinkling, in amounts which vary with the weather conditions or crop conditions.

Compared with the prior art, the invention has the beneficial effects that: 1. compared with a single agent, the compound has obvious synergistic effect, and the control effect is improved; 2. the composition consists of two active ingredients with different action mechanisms, and is favorable for overcoming and delaying the generation of drug resistance of pathogenic bacteria; 3. the dosage is reduced by mixing the medicament, so that the cost is reduced and the pollution to the environment is reduced; 4. not only has effect on plant fungal crop diseases, but also can induce and treat plant viral diseases. The invention has better control effect on virus diseases and bacterial and fungal diseases such as tobacco virus diseases, black shank diseases, tomato early blight, late blight, cucumber downy mildew, pepper phytophthora blight, damping off, peanut brown spot, black spot and the like.

Detailed Description

In order to make the technical problems, solutions and advantages to be solved by the embodiments of the present invention clearer, specific embodiments will be described in detail below, but the present invention is by no means limited to these examples. The following description is only a preferred embodiment of the present invention, and is only for the purpose of explaining the present invention, and should not be construed as limiting the scope of the present invention. It should be understood that any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

The pesticide prepared by combining the effective components of different pesticides is an effective and quick way for developing and researching new pesticides and preventing and controlling resistant germs in agriculture at present. Pesticides of different species, when mixed, typically exhibit a number of three types of action: additive action, synergistic action and antagonistic action. The compound synergistic formula is good, can obviously improve the actual prevention and treatment effect, and reduces the using amount of pesticide, thereby being beneficial to delaying the generation speed of drug resistance of germs and being an important means for comprehensively preventing and treating diseases.

The composition of the invention takes the methicillin-inducing amine (A) as one effective component and the amide fungicide (B) as the other effective component, and the combination of the two components has obvious synergistic effect on plant fungal diseases and viral diseases, rather than simple superposition of the effects of the two medicaments, which can be clearly understood from the following test results.

In order to prevent and treat cucumber downy mildew in agricultural production, the inventor carries out synergistic research of mutual compounding of methicillin-inducing amine and amide bactericides dimethomorph or metalaxyl, and the specific method comprises the following steps:

the test uses downy mildew as the test object. The raw medicines are prepared into required test medicaments, and the test method refers to agricultural industry standard of the people's republic of China. Firstly, 5 different concentration gradients are set for a single agent and each mixed agent (on the basis of a preliminary test result, the concentration setting is different according to different agent combinations and proportions, and the bacteriostasis rate is set within the range of 5-90%). Repeat 3 times with clear water control. Inoculating the prepared peronospora parasitica spore liquid to the center of each processing leaf by adopting a semi-living body method (a plate leaf method), and culturing for four days in a constant-temperature constant-humidity incubator at 25 ℃; the plaque diameter was examined (cross method), the percentage of inhibition of plaque growth was calculated for each treatment, the EC50 value for each drug was determined by linear regression analysis between the rate of inhibition and the logarithm of the series of concentrations, and the co-toxicity coefficient (CTC) of the combination was calculated from the table of co-toxicity coefficients, thereby evaluating the activity of the test agent against pathogens.

The co-toxicity coefficient (CTC) of the compound preparation is not less than 120, which shows a synergistic effect; CTC ≦ 80 shows antagonism; 80< CTC <120 shows additive effects.

TABLE 1 indoor potency assay of methicillin-induced amine and dimethomorph combinations for cucumber downy mildew

Drug treatment	Regression equation of virulence	EC₅₀(mg/L)	Co-toxicity coefficient (CTC)
				Methothiazole amine (A)	Y＝1.7344+1.9691X	45.5450	——
Dimethomorph (B)	Y＝1.7509+2.0479X	38.6011	——
				A：B＝1:30	Y＝2.8009+1.5354X	27.0529	143.39
A：B＝1:15	Y＝2.9474+1.5828X	19.8187	196.65
				A：B＝1:7	Y＝2.9509+1.6734X	16.7708	234.64
A：B＝1:1	Y＝2.9690+1.7170X	15.2371	274.24
				A：B＝10:1	Y＝2.8132+1.6267X	22.0934	202.83
A：B＝20:1	Y＝2.8010+1.5893X	24.1912	186.67
				A：B＝40:1	Y＝2.8009+1.5354X	27.0529	167.62

TABLE 2 indoor toxicity assay for cucumber downy mildew by compounding methicillin-inducing amine and metalaxyl

Drug treatment	Regression equation of virulence	EC₅₀(mg/L)	Co-toxicity coefficient (CTC)
				Methothiazole amine (A)	Y＝1.7344+1.9691X	45.5450	——
Metalaxyl (F)	Y＝2.2672+1.5501X	57.9453	——
				A：F＝1:30	Y＝2.4845+1.5534X	41.6248	137.99
A：F＝1:15	Y＝2.4753+1.5969X	38.1095	149.51
				A：F＝1:7	Y＝2.9400+1.5383X	21.8358	256.63
A：F＝1:1	Y＝2.9711+1.5701X	19.5965	260.26
				A：F＝10:1	Y＝2.9794+1.4297X	25.8997	179.34
A：F＝20:1	Y＝2.7247+1.5272X	30.8931	148.95
				A：F＝40:1	Y＝2.4394+1.6388X	36.5165	125.38

Indoor toxicity test results show that the compounding of the methicillin-induced amine and the dimethomorph and the compounding of the methicillin-induced amine and the metalaxyl have obvious synergistic effect on cucumber downy mildew, and the control effect is better than that of a single-dose variety. The inventor tests that the compounding of the methicillin-inducing amine and other amide bactericides (such as boscalid, thifluzamide, furametpyr and the like) has a similar effect on cucumber downy mildew.

In order to prevent and treat tomato late blight in agricultural production, the inventor carries out synergistic research of mutual compounding of methicillin-inducing amine and an amide bactericide-boscalid, and the specific method comprises the following steps: the test uses tomato late blight as a test object. The raw medicines are prepared into required test medicaments, and the test method refers to agricultural industry standard of the people's republic of China. Firstly, 5 different concentration gradients are set for a single agent and each mixed agent (on the basis of a preliminary test result, the concentration setting is different according to different agent combinations and proportions, and the bacteriostasis rate is set within the range of 5-90%). Repeat 3 times with clear water control. Inoculating in a pot culture method, and culturing in a constant temperature and humidity incubator at 28 deg.C for 4 d; the plaque diameter was examined (cross method), the percentage of inhibition of plaque growth was calculated for each treatment, the EC50 value for each drug was determined by linear regression analysis between the rate of inhibition and the logarithm of the series of concentrations, and the co-toxicity coefficient (CTC) of the combination was calculated from the table of co-toxicity coefficients, thereby evaluating the activity of the test agent against pathogens.

TABLE 3 indoor determination of efficacy of methicillin-inducing amine and boscalid in combination on tomato late blight

Drug treatment	Regression equation of virulence	EC₅₀(mg/L)	Co-toxicity coefficient (CTC)
				Methothiazole amine (A)	Y＝1.7344+1.9691X	45.5450	——
Boscalid (C)	Y＝2.6278+1.5349X	35.1207	——
				A：C＝1:30	Y＝2.9480+1.7181X	15.6444	226.17
A：C＝1:15	Y＝3.0215+1.7140X	14.2664	249.75
				A：C＝1:7	Y＝3.1129+1.7219X	12.4706	289.92
A：C＝1:1	Y＝3.2078+1.7559X	10.4874	378.16
				A：C＝10:1	Y＝2.8305+1.7827X	16.4803	269.09
A：C＝20:1	Y＝2.8312+1.7346X	17.7962	252.36
				A：C＝40:1	Y＝2.8451+1.6902X	18.8316	240.12

Indoor toxicity test results show that the compounding of the methicillin-inducing amine and the boscalid has obvious synergistic effect on the tomato late blight, and the control effect is better than that of a single-dose variety. The inventor finds that the combination of the methicillin and other amide bactericides (such as dimethomorph, thifluzamide, furametpyr, metalaxyl and the like) has similar effect on the tomato late blight.

In order to prevent and treat rice sheath blight disease in agricultural production, the inventor carries out synergistic research of mutual compounding of thifluzamide and amide fungicide furametpyr, and the specific method comprises the following steps: the test adopts sheath blight as a test object. The raw medicines are prepared into required test medicaments, and the test method refers to agricultural industry standard of the people's republic of China. Firstly, 5 different concentration gradients are set for a single agent and each mixed agent (on the basis of a preliminary test result, the concentration setting is different according to different agent combinations and proportions, and the bacteriostasis rate is set within the range of 5-90%). Repeat 3 times with clear water control. Inoculating in a pot culture method, and culturing in a constant temperature and humidity incubator at 28-30 deg.C for 2 d; the activity of the test agents against pathogens was evaluated by examining the lesion length, calculating the percent inhibition of lesion growth for each treatment, determining the EC50 value for each agent by linear regression analysis between the rate of inhibition and the log of the series of concentrations, and calculating the co-toxicity coefficient (CTC) of the combination using the table of co-toxicity coefficients.

TABLE 4 indoor determination of efficacy of methicillin-induced amine and furametpyr in combination on rice sheath blight disease

Drug treatment	Regression equation of virulence	EC₅₀(mg/L)	Co-toxicity coefficient (CTC)
				Methothiazole amine (A)	Y＝1.7344+1.9691X	45.5450	——
Furametpyr (E)	Y＝4.0225+0.6603X	30.2333	——
				A：E＝1:30	Y＝4.1857+0.6617X	17.0025	179.77
A：E＝1:15	Y＝4.2189+0.6590X	15.3213	201.56
				A：E＝1:7	Y＝4.2531+0.6699X	13.0309	242.19
A：E＝1:1	Y＝4.2618+0.6771X	12.3084	295.26
				A：E＝10:1	Y＝4.2123+0.6684X	15.0811	288.69
A：E＝20:1	Y＝4.0622+0.7110X	20.8401	213.40
				A：E＝40:1	Y＝3.9927+0.7084X	26.4243	170.26

Indoor toxicity test results show that the compounding of the thizamide and the furametpyr has obvious synergistic effect on rice sheath blight and better control effect than single-dose varieties. The inventor finds that the compounding of the methicillin-inducing amine and other amide bactericides (such as dimethomorph, boscalid, thifluzamide, metalaxyl and the like) has similar effect on rice sheath blight disease through tests.

In order to prevent and treat tobacco virus diseases in agricultural production, the inventor carries out synergistic research of mutual compounding of methyl thiazole induced amine and amide bactericide-thiaflutolanil, and the specific method comprises the following steps: the tobacco virus disease is used as a test object in the test. The raw medicines are prepared into required test medicaments, and the test method refers to agricultural industry standard of the people's republic of China. Firstly, 5 different concentration gradients are set for a single agent and each mixed agent (on the basis of a preliminary test result, the concentration setting is different according to different agent combinations and proportions, and the bacteriostasis rate is set within the range of 5-90%). Repeat 3 times with clear water control. Inoculating tobacco viruses in each treatment by adopting a pot culture method, and culturing for 5 days in a constant-temperature constant-humidity incubator at the temperature of 28-30 ℃; the number of diseased spots on the inoculated leaf for each treatment was checked, the percentage of inhibition of growth of the spots for each treatment was calculated, the EC50 value of each drug was determined by linear regression analysis between the probability value of the inhibition and the logarithmic value of the series of concentrations, and the co-toxicity coefficient (CTC) of the drug combination was calculated from the co-toxicity coefficient table, thereby evaluating the activity of the test agent against pathogens.

TABLE 5 indoor determination of efficacy of methicillin-inducing amine and thifluzamide combinations on viral diseases

Indoor toxicity test results show that the compounding of the thiabendazole and the thifluzamide has obvious synergistic effect on tobacco virus diseases, and the prevention and treatment effect is better than that of a single-dose variety. The inventor tests that the composite of the methicillin and other amide bactericides (such as dimethomorph, boscalid, furametpyr, metalaxyl and the like) has similar effect on the tobacco virus diseases.

The bactericidal composition can be prepared into any one dosage form suitable for agriculture by using a known method, and the better dosage forms are wettable powder, water suspending agent, oil suspending agent and water dispersible granules. The invention is further illustrated by the following examples, in which all the formulations are given in weight percent. The processing technology of various dosage forms of the composition is the prior art, and can be changed according to different conditions.

Example 1: 32% thiiramide furametpyr suspension

Weighing 2% of methidamine, 30% of furametpyr, 4% of sodium polycarboxylate, 3% of fatty alcohol-polyoxyethylene ether, 6% of ethylene glycol, 0.35% of XG, 0.6% of organic silicon defoamer, 0.4% of sodium benzoate and deionized water respectively until the weight is 100%. Mixing the components, uniformly shearing and mixing the components at a high speed, and sanding the mixture for 2 to 3 hours by using a sand mill to obtain the suspension of the 32 percent of thiamine induced amine and furametpyr with the fineness of 1 to 5 microns.

Furametpyr in the present embodiment may be replaced with other amide bactericides (such as dimethomorph, boscalid, thifluzamide, metalaxyl and the like) to form a new embodiment.

Example 2: 30% thizamide furametpyr suspending agent

15% of methidamine, 15% of furametpyr, 4% of sodium naphthalenesulfonate, 2% of nonylphenol polyoxyethylene phosphate, 6% of ethylene glycol, 0.30% of XG, 0.6% of an organosilicon antifoaming agent, 0.4% of sodium benzoate and deionized water are respectively weighed to 100% by weight. Mixing the components, uniformly shearing and mixing at a high speed, and sanding for 2-3 hours by using a sand mill to obtain the suspension with the fineness of 1-5 microns, namely the 30% thizamide furametpyr suspension.

Example 4: 46.5% thiiramide furametpyr suspension

1.5% of methidamine, 45% of furametpyr, 4% of sodium polycarboxylate, 3% of fatty alcohol-polyoxyethylene ether, 6% of ethylene glycol, 0.35% of XG, 0.6% of silicone defoamer, 0.4% of sodium benzoate and deionized water are respectively weighed and added to 100% by weight. Mixing the components, uniformly mixing by high-speed shearing, and sanding for 2-3 hours by a sand mill to obtain the suspension containing 46.5% of thifluzamide and furametpyr, wherein the fineness of the suspension is 1-5 microns.

Example 5: 24% methine luramine dimethomorph water dispersible granule

Weighing 3% of methine luramine, 21% of dimethomorph, 5% of sodium dodecyl sulfate, 2% of modified lignin, 2% of calcium chloride and kaolin to make up to 100% by weight. Mixing the components, mechanically crushing, then crushing by airflow, uniformly mixing, then granulating by a wet method and drying to obtain the 24% methine luramine dimethomorph water dispersible granule.

The dimethomorph in the embodiment can be replaced by other amide bactericides (such as furametpyr, boscalid, thifluzamide, metalaxyl and the like) to form a new embodiment.

Example 6: 31.5% methine luramine dimethomorph suspoemulsion

Weighing 1.5% of dimethomorph, 3% of phosphate polyoxyethylene ether, 2% of nonylphenol polyoxyethylene ether and 8% of nonaromatic solvent, uniformly mixing and stirring for later use, and weighing 30% of methyl-thiin induced amine, 3% of phosphate polyoxyethylene ether, 6% of glycol, 0.25% of XG, 0.6% of organic silicon defoamer, 0.4% of sodium benzoate and deionized water to 100% by weight. Grinding to fineness of 1-4 μm, adding prepared dimethomorph solution, shearing with high shear disperser for 30min, and mixing to obtain 31.5% suspending emulsion.

Example 7: 30% wettable powder of methine luramine and dimethomorph

Weighing 15% of methine induced amine, 15% of dimethomorph, 7% of modified lignin, 3% of polyoxyethylene ether formaldehyde condensate, 5% of white carbon black and kaolin to complement to 100% by weight; the components are mixed, mechanically crushed, airflow crushed and mixed evenly to obtain the 30 percent wettable powder of the methine luramine and dimethomorph.

Example 8: 41% methine luramine-thiaflunamide water dispersible granule

Weighing 1% of methine luramine, 40% of thiaflutolamide, 5% of sodium dodecyl sulfate, 2% of modified lignin, 2% of calcium chloride and kaolin to make up to 100% by weight. The components are mixed, mechanically crushed, airflow crushed, evenly mixed, wet granulated and dried to obtain the 41 percent of methidamine-thifluzamide water dispersible granule.

The dimethomorph in the embodiment can be replaced by other amide bactericides (such as furametpyr, boscalid, metalaxyl and the like) to form a new embodiment.

Example 9: 31% thizamide thifluzamide suspension emulsion

Weighing 1% of thiaflunomide, 3% of phosphate ester polyoxyethylene ether, 2% of nonylphenol polyoxyethylene ether and 8% of nonaromatic solvent, uniformly mixing and stirring for later use, weighing 30% of methyl thia induced amine, 3% of phosphate ester polyoxyethylene ether, 6% of ethylene glycol, 0.25% of XG, 0.6% of organic silicon defoamer, 0.4% of sodium benzoate and deionized water to 100% by weight. After the fineness of the suspension emulsion is 1-4 microns by sanding, the prepared dimethomorph solution is added, and the suspension emulsion is sheared for 30min by a high shear disperser and then mixed evenly to prepare the 31 percent suspension emulsion of the thiamine induced amine and the thiafluzamide.

The pesticide preparation prepared by the above embodiment is used for preventing and treating rice sheath blight disease:

1. and (3) test treatment: the test is carried out according to the standard of the people's republic of China (pesticide field efficacy test criteria). The dosage of the test medicament is respectively provided with three treatment concentrations according to different components. The control agents were the commercial pesticides 25% methidaluramine and 15% furametpyr WP and the blank water test, respectively.

2. The test method comprises the following steps: each cell area is 50m²The method is repeated for 4 times, and the method for investigating before application and the pesticide effect after prevention and treatment comprises the following steps: points are taken per cell diagonal, and 10 clumps of surveys are drawn per point. The test results are given belowTable 6:

TABLE 6 the control effect of the compounding of methicillin-inducing amine and furametpyr on rice sheath blight

The field efficacy results show that the compounding of the thizamide and the furametpyr has excellent prevention and treatment effect on rice sheath blight, and the prevention and treatment effect is better than that of a single-dose variety.

The pesticide preparation prepared in the above example is used for controlling tobacco black shank: 1. and (3) test treatment: the test refers to the standard of the people's republic of China (pesticide field efficacy test criteria)

The process is carried out. The dosage of the test medicament is respectively set with three treatment concentrations according to different components, and the reference medicaments are respectively a commercial pesticide of 25 percent of methine luramine and 50 percent of dimethomorph WG and a blank clear water test.

2. The test method comprises the following steps: each cell area is 50m²The method is repeated for 4 times, and the method for investigating before application and the pesticide effect after prevention and treatment comprises the following steps: samples were taken diagonally per cell, 10 plants per spot. The lesion area per whole stem was rated as a percentage of the total stem area, and the results are shown in Table 7 below:

TABLE 7 preventive and therapeutic effects of methicillin and dimethomorph on tobacco black shank

The field efficacy results show that the compounding of the methicillin-resistant amine and the dimethomorph also has excellent control effect on the tobacco black shank, and the control effect is better than that of a single-dose variety.

The pesticide preparation prepared in the above example is used for controlling cucumber downy mildew test: 1. and (3) test treatment: the test is carried out according to the standard of the people's republic of China (pesticide field efficacy test criteria). The dosage of the test medicament is respectively set with three treatment concentrations according to different components, and the reference medicaments are respectively a commercial pesticide of 25 percent of methine luramine and 50 percent of dimethomorph WG and a blank clear water test.

2. The test method comprises the following steps: each cell area is 50m²The method is repeated for 4 times, and the method for investigating before application and the pesticide effect after prevention and treatment comprises the following steps: samples were taken diagonally per cell, 10 plants per spot. The lesion area per whole leaf was rated as a percentage of the total leaf area, and the results are shown in Table 8 below:

TABLE 8 control Effect of methicillin-induced amine and dimethomorph on cucumber downy mildew

The field efficacy results show that the compounding of the methicillin-resistant attractant and the dimethomorph also has excellent control effect on cucumber downy mildew, and the control effect is better than that of a single-dose variety.

The pesticide preparation prepared by the above embodiment is used for preventing and treating tobacco virus diseases: 1. and (3) test treatment: the test is carried out according to the standard of the people's republic of China (pesticide field efficacy test criteria). The dosage of the test medicament is respectively set with three treatment concentrations according to different components, and the reference medicaments are respectively a commercial pesticide of 25 percent of methyl thia-luramine and 24 percent of thiafluzamide SC and a blank clear water test.

TABLE 9 preventive and therapeutic effects of compounding of methicillin-inducing amine and thiaflutolanil on tobacco virus diseases

The field efficacy results show that the compounding of the methyl thia-luride and the thiaflutolanil also has excellent prevention and treatment effects on tobacco virus diseases, and the prevention and treatment effects are better than those of single-dose varieties.

Claims

1. A germicidal composition, characterized by: the active ingredients of the bactericidal composition comprise methicillin-induced amine and amide bactericides, wherein the amide bactericides are furametpyr, and the weight ratio of the methicillin-induced amine to the amide bactericides is 1: 30-40: 1.

2. the germicidal composition of claim 1, wherein: the weight ratio of the methyl thiadiazole induced amine to the amide fungicide is 1: 15-20: 1.

3. the germicidal composition of claim 1, wherein: the weight ratio of the methyl thiadiazole induced amine to the amide fungicide is 1: 7-10: 1.

4. the germicidal composition as claimed in any one of claims 1 to 3, wherein: the bactericidal composition also contains auxiliary components of pesticide preparations so as to be prepared into dosage forms suitable for agriculture.

5. The germicidal composition of claim 4, wherein: the auxiliary components of the pesticide preparation are one or more of a solvent, an emulsifier, a dispersant, a stabilizer, an antifreezing agent or a wetting agent.

6. The germicidal composition of claim 5, wherein: the dosage form of the bactericidal composition is wettable powder, water dispersible granules, suspending agent or dry suspending agent.

7. Use of the germicidal composition as claimed in claim 1, characterized in that: is used for preventing and treating plant diseases.

8. Use of a fungicidal composition according to claim 7, characterized in that: can be used for preventing and treating tobacco virus disease, black shank, tomato early blight, late blight, cucumber downy mildew, pepper blight, damping off, peanut brown spot, and black spot.