CN109439702A - The technique for handling threonine high gravity fermentation waste water - Google Patents
The technique for handling threonine high gravity fermentation waste water Download PDFInfo
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- CN109439702A CN109439702A CN201811213739.5A CN201811213739A CN109439702A CN 109439702 A CN109439702 A CN 109439702A CN 201811213739 A CN201811213739 A CN 201811213739A CN 109439702 A CN109439702 A CN 109439702A
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- threonine
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- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 title claims abstract description 61
- 239000004473 Threonine Substances 0.000 title claims abstract description 61
- 239000002921 fermentation waste Substances 0.000 title claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000005484 gravity Effects 0.000 title claims abstract description 10
- 238000000855 fermentation Methods 0.000 claims abstract description 50
- 230000004151 fermentation Effects 0.000 claims abstract description 50
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 239000005416 organic matter Substances 0.000 claims abstract description 16
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims abstract description 12
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 12
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 12
- 235000014103 egg white Nutrition 0.000 claims abstract description 12
- 210000000969 egg white Anatomy 0.000 claims abstract description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 36
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- 241000195597 Chlamydomonas reinhardtii Species 0.000 claims description 9
- 239000004202 carbamide Substances 0.000 claims description 9
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 8
- 239000013049 sediment Substances 0.000 claims description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- 238000005119 centrifugation Methods 0.000 claims description 6
- 239000013078 crystal Substances 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 239000012452 mother liquor Substances 0.000 claims description 6
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000004021 humic acid Substances 0.000 claims description 5
- 230000001376 precipitating effect Effects 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- 238000000108 ultra-filtration Methods 0.000 claims description 5
- 206010013786 Dry skin Diseases 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 claims description 3
- 108091005508 Acid proteases Proteins 0.000 claims description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 3
- 101100465553 Dictyostelium discoideum psmB6 gene Proteins 0.000 claims description 3
- 102000016943 Muramidase Human genes 0.000 claims description 3
- 108010014251 Muramidase Proteins 0.000 claims description 3
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 3
- 101100169519 Pyrococcus abyssi (strain GE5 / Orsay) dapAL gene Proteins 0.000 claims description 3
- 241000223261 Trichoderma viride Species 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 3
- 239000000908 ammonium hydroxide Substances 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 101150011371 dapA gene Proteins 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000005469 granulation Methods 0.000 claims description 3
- 230000003179 granulation Effects 0.000 claims description 3
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 3
- 229960000274 lysozyme Drugs 0.000 claims description 3
- 239000004325 lysozyme Substances 0.000 claims description 3
- 235000010335 lysozyme Nutrition 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 230000003519 ventilatory effect Effects 0.000 claims description 3
- 235000020985 whole grains Nutrition 0.000 claims description 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 claims description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- 210000002421 cell wall Anatomy 0.000 claims description 2
- 238000001704 evaporation Methods 0.000 claims description 2
- 230000008020 evaporation Effects 0.000 claims description 2
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 claims description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000012545 processing Methods 0.000 abstract description 6
- 239000002699 waste material Substances 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 5
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 230000002829 reductive effect Effects 0.000 abstract description 2
- 239000010865 sewage Substances 0.000 abstract description 2
- 238000002203 pretreatment Methods 0.000 abstract 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 24
- 150000001413 amino acids Chemical class 0.000 description 13
- 239000003337 fertilizer Substances 0.000 description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 8
- 241000588724 Escherichia coli Species 0.000 description 8
- 229910052799 carbon Inorganic materials 0.000 description 8
- 239000002351 wastewater Substances 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 5
- 241000227653 Lycopersicon Species 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000005453 pelletization Methods 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- BIGPRXCJEDHCLP-UHFFFAOYSA-N ammonium bisulfate Chemical compound [NH4+].OS([O-])(=O)=O BIGPRXCJEDHCLP-UHFFFAOYSA-N 0.000 description 1
- 241000953178 bacterium K12 Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- VSEWZMQQQHLHQT-UHFFFAOYSA-N manganese;sulfuric acid;hydrate Chemical compound O.[Mn].OS(O)(=O)=O VSEWZMQQQHLHQT-UHFFFAOYSA-N 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Pest Control & Pesticides (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention belongs to technical field of biological environmental protection, disclose the technique of processing threonine high gravity fermentation waste water, it includes the following steps: that step 1) prepares threonine fermentation liquid, step 2 prepares egg white icing, step 3) prepares threonine, step 4) collects Threonine Fermentation waste water, and step 5) prepares biological organic matter.Threonine, egg white icing and biological organic matter are prepared simultaneously in fermentation process of the present invention, intermediate pre-treatment step is omitted, realizes the direct biological utilisation of sewage, discharge, remarkable in economical benefits is also reduced while turning waste into wealth.
Description
Technical field
The invention belongs to technical field of biological environmental protection, and in particular to the technique of processing threonine high gravity fermentation waste water.
Background technique
Threonine is a kind of essential amino acid, is the third limitation of the second limiting amino acid and poultry feed of pannage
Amino acid, with the extensive use of lysine, methionine composite in mixed feed, threonine, which is increasingly becoming, influences livestock and poultry life
Long major limiting factors.Threonine is mainly used as feed addictive, and production stage is concentration, crystallization, extracts thallus egg
It is white, and the mother liquid disposal difficulty generated during it is larger, higher cost, it is difficult to obtain the relatively high byproduct of sexual valence.
The waste water fraction of amino acid fermentation industry at present is used for the exploitation of liquid fertilizer, and there are also very little parts to be used for solid
The slurry-spraying pelletizing technique of organic fertilizer.But slurry-spraying pelletizing needs liquid measure few, cannot consume a large amount of amino acids industry waste liquid.Together
When also due to liquid fundamental characteristics, there is such as acid compared with strong, corrosivity compared with strong, foul smell is heavier, amino acid waste liquid ingredient
Be difficult to optimize, field efficacy is bad, even to negative factors such as certain crop nocuousness, make amino acid waste liquid in terms of organic fertilizer
Utilization be constantly subjected to limit, fertilizer produced, either solid waste or liquid fertilizer are all difficult to promote.These are all
Inhibit recycling for amino acids industry waste water.With the development of amino acids industry, the amount of generated amino acid wastewater
It is more and more.It is more and more in short supply to belong to water resource, environmental protection pressure is increasing, and the processing of amino acids industry waste water is also more next
More become a great problem of puzzlement amino acids industry development.
Amino acid fermentation waste liquid still contains a large amount of amino acid residues and organic matter residual.This aspect makes amino acid
Waste water COD is higher, deals with very difficult, on the other hand has the potentiality utilized in terms of planting industry again.Mesh
Before, microbial biotechnology can realize the conversion for having feature, shape using the metabolic function of microorganism to the organic principle in material
At the secondary metabolites for having feature, and change the physical chemistry characteristic and biochemical characteristics of material.Certain micro-organisms again can be right simultaneously
The growth of crops generates facilitation, plays the role of promoting respiration capability to soil, brings soil improvement and vegetable fertilizer
Effect is the main force of microbial manure.Patented technology before applicant improves Threonine Fermentation waste liquid, wherein
Fermentation wastes are prepared for solid by " a kind of fertilizer prepared using Threonine Fermentation waste " while preparing threonine
Body fertilizer achieves preferable economic benefit；" compound fertilizer prepared using Threonine Fermentation waste " utilizes fermentation wastes
It is prepared for microbial-bacterial fertilizer.
Summary of the invention
The object of the present invention is to provide the techniques of processing threonine high gravity fermentation waste water, and the technique is in preparation Soviet Union ammonia
It is prepared for mycoprotein cream while sour, and has handled high-concentration waste water, liquid fertilizer is prepared using high-concentration waste water, it is real
Having showed turns waste into wealth, and kills two birds with one stone.
For achieving the above object, the invention adopts the following technical scheme:
The technique for handling threonine high gravity fermentation waste water comprising following steps: step 1) prepares threonine fermentation liquid, step
2) egg white icing is prepared, step 3) prepares threonine, and step 4) collects Threonine Fermentation waste water, and step 5) prepares biological organic matter.
Further, the step 1) prepares threonine fermentation liquid, includes the following steps: that threonine Escherichia coli work will be produced
Journey bacterium K12 △ dapA seed liquor is linked into the fermentor containing fermentation medium according to 8% inoculum concentration ferments, temperature
30 DEG C, tank pressure is 0.04MPa, ventilatory capacity 0.5vvm, revolving speed 100rpm, fermentation time 36h, is then connect according to 10%
Kind amount access Chlamydomonas reinhardtii, continues fermented and cultured 36h, stops fermenting, collect threonine fermentation liquid.
Further, the step 2 prepares egg white icing, include the following steps: threonine fermentation liquid first pass around disk from
Scheming is centrifuged 5min with 4000rpm, collects supernatant liquid and mycoprotein precipitating, and mycoprotein precipitating is added to reactor,
And appropriate warm water is added and mixes well, solid content 10% is adjusted, adjusts 55 DEG C of enzymolysis temperature, adds a little sulfuric acid adjustment pH5.5, point
Jia Ru ten thousand U/g of lysozyme 10kg/m3(1), ten thousand U/g of acid protease 25kg/m3(10), it is slowly stirred, enzymolysis time 6h,
Disk plate centrifuge is used to be centrifuged 3min with 5000rpm, separation removal cell wall steams supernatant through triple effect plate-type evaporator low temperature
Being sent to solid content is 60%, and egg white icing is made in the direct barrelling of gained paste.
Further, the step 3) prepares threonine, includes the following steps: that (10,000 Da are cut supernatant liquid by ceramic membrane
Stay molecular weight) filtering, filtered solution and trapped substance are collected, filtered solution is separated through decanter centrifuge, centrifugal speed 5000rpm,
Centrifugation time is 3min, collects supernatant (protein content is less than 0.5%) and sediment；Then pass through ultrafiltration membrance filter, collect filter
Liquid is crossed, ultrafiltration retaining molecular weight is 300Da；The intermittent single-action condensing crystallizing pot of filtered solution is crystallized, crystal is collected by centrifugation
And mother liquor, it is 0.8% that crystal, which is then placed in 120 DEG C of dryings to moisture content, slabbing is compressed, then put into granulation tower,
It is in fluidized state under the action of thermal current；65 DEG C of fluidized bed dryings, then through broken whole grain to get threonine.
Further, the step 4) collects Threonine Fermentation waste water, includes the following steps: to retain obtained by step 3)
Object, sediment and mother liquor mixing, obtain Threonine Fermentation waste water.
Further, the step 5) prepares biological organic matter, includes the following steps: for Threonine Fermentation waste water to be heated to
, 10min being handled under heat-retaining condition, is then cooled to room temperature, it is 6.5 that ammonium hydroxide adjustment pH, which is added, is then successively inoculated with withered grass by 100 DEG C
(concentration is 1 × 10 to bacillus8Cfu/mL) and trichoderma viride (concentration be 1 × 108Cfu/mL fermentation process, inoculation) are carried out
Amount is 5%, and fermentation temperature is 32 DEG C, and fermentation time is 3 days, terminates fermentation, into fermentation liquid by addition potassium dihydrogen phosphate, corruption
Acid and urea are grown, is filtered after being uniformly mixed, collects filtrate to get biological organic matter is arrived.
Preferably, the component of the fermentation medium are as follows: glucose 20g/L, glycerol 15g/L, corn pulp 15g/L, sulfuric acid
Ammonium 2g/L, potassium dihydrogen phosphate 0.2g/L, dipotassium hydrogen phosphate 0.2g/L, epsom salt 0.1g/L, ferrous sulfate heptahydrate 0.01g/
L, manganese sulfate monohydrate 0.01g/L, pH value 6.5.
Preferably, in the step 5), fermentation liquid, potassium dihydrogen phosphate, humic acid and urea ratio be 1L:5g:3g:
2g。
The beneficial effect of starting point and acquirement that the present invention studies mainly includes but is not limited to the following aspects:
Fermenting carbon source selection glucose and glycerol of the present invention, earlier fermentation, cell density is low, and oxygen-supplying amount is sufficient, and Escherichia coli are excellent
First with glucose as carbon source, the generation of growing microorganism and threonine can be promoted；It fermenting the middle and later periods, glucose is depleted,
Escherichia coli use glycerol as carbon source at this time, and since the rate that cell absorbs glycerol is lower, the carbon flow into glycolysis declines,
To reduce the accumulation of acetic acid, while improving the yield of threonine；
The present invention can carry out non-light and work as carbon source using the acetic acid in fermentation liquid by being inoculated with Chlamydomonas reinhardtii in fermentation
With, and it is more difficult use glycerol as carbon source, thus relieve to Escherichia coli produce threonine inhibiting effect, additionally it is possible to carry out micro-
The photosynthesis of amount discharges oxygen, for Escherichia coli fermentation produce threonine come using.By adding Chlamydomonas reinhardtii, it is not only able to mention
The yield of high threonine, and mycoprotein yield also correspondinglys increase；
Threonine, egg white icing and biological organic matter are prepared simultaneously in fermentation process of the present invention, intermediate pretreatment step is omitted
Suddenly, the direct biological utilisation for realizing sewage, also reduces discharge, remarkable in economical benefits while turning waste into wealth；
The present invention obtains mycoprotein cream, alternative commercially available yeast powder product, thus significantly while preparing threonine
Fermentation costs are reduced, the added value of industry is improved.
The present invention is handled fermentation waste water during preparing biological organic matter, using composite bacteria liquid, to COD, ammonia
Nitrogen is degraded, and generates active material, is conducive to plant growth, also there is certain preventive and therapeutic effect to pest and disease damage；Humic acid
Containing functional groups such as carboxyl, phenolic hydroxyl groups, there are stronger ion-exchange capacity and adsorption capacity, there is apparent synergy to make in urea
With, complex compound can be generated with urea, urea decomposition can be slowed down, reduce and volatilize, extension fertilizer efficiency.
Specific embodiment
Those skilled in the art can use for reference present disclosure, be suitably modified realization of process parameters.In particular, it should be pointed out that
All similar substitutions and modifications are apparent to those skilled in the art, they are considered as being included in this hair
It is bright.Product and method of the invention is described by preferred embodiment, and related personnel can obviously not depart from this hair
Product as described herein and method are modified in bright content, spirit and scope or appropriate changes and combinations, to realize and answer
Use the technology of the present invention.For a further understanding of the present invention, the following describes the present invention in detail with reference to examples.
Embodiment 1
The technique for handling threonine high gravity fermentation waste water comprising following steps:
By colibacillus engineering K12 △ dapA seed liquor, (concentration of seed liquor is 1 × 10 to step 1)8Cfu/mL) according to 8%
Inoculum concentration, which is linked into the fermentor containing fermentation medium, ferments, and 30 DEG C of temperature, tank pressure is 0.04MPa, and ventilatory capacity is
0.5vvm, revolving speed 100rpm, fermentation time 36h, then according to 10% inoculum concentration access Chlamydomonas reinhardtii (Chlamydomonas reinhardtii
Concentration is 1 × 105Cfu/mL), continue fermented and cultured 36h, stop fermentation, collect fermentation liquid；
The component of the fermentation medium are as follows: glucose 20g/L, glycerol 15g/L, corn pulp 15g/L, ammonium sulfate 2g/L, phosphoric acid
Potassium dihydrogen 0.2g/L, dipotassium hydrogen phosphate 0.2g/L, epsom salt 0.1g/L, ferrous sulfate heptahydrate 0.01g/L, sulfuric acid monohydrate
Manganese 0.01g/L, pH value 6.5；
Step 2 fermentation liquid first passes around disk plate centrifuge and is centrifuged 5min with 4000rpm, collects supernatant liquid and mycoprotein is heavy
It forms sediment, mycoprotein precipitating is added to reactor, and appropriate warm water is added and mixes well, adjusts solid content 10%, adjust enzyme digestion reaction
55 DEG C of temperature, add a little sulfuric acid adjustment pH5.5, be separately added into ten thousand U/g of lysozyme 10kg/m3(1), acid protease 25kg/m3
(100,000 U/g), is slowly stirred, enzymolysis time 6h, and disk plate centrifuge is used to be centrifuged 3min, separation removal cell with 5000rpm
Wall, by supernatant through triple effect plate-type evaporator low-temperature evaporation to solid content be 60%, enzymatic hydrolysis is made in the direct barrelling of gained paste
Egg white icing；
Step 3) supernatant liquid is filtered by ceramic membrane (10,000 Da molecular cut off), filtered solution and trapped substance is collected, by filtered solution
It is separated through decanter centrifuge, centrifugal speed 5000rpm, centrifugation time 3min, collecting supernatant, (protein content is less than
And sediment 0.5%)；Then pass through ultrafiltration membrance filter, collect filtered solution, ultrafiltration retaining molecular weight is 300Da；By filtered solution
It is crystallized with intermittent single-action condensing crystallizing pot, crystal and mother liquor is collected by centrifugation, crystal is then placed in 120 DEG C of drying to moisture and is contained
Amount is 0.8%, compresses slabbing, then put into granulation tower, is in fluidized state under the action of thermal current；65 DEG C of fluidized beds are dry
It is dry, then through broken whole grain to get threonine；
Step 4) mixes trapped substance obtained by step 3), sediment and mother liquor, obtains Threonine Fermentation waste water；
Threonine Fermentation waste water is heated to 100 DEG C by step 5), is handled 10min under heat-retaining condition, is then cooled to room temperature, and is added
It is 6.5 that ammonium hydroxide, which adjusts pH, and being then successively inoculated with bacillus subtilis, (concentration is 1 × 108) and trichoderma viride (concentration cfu/mL
It is 1 × 108Cfu/mL fermentation process) is carried out, inoculum concentration is 5%, and fermentation temperature is 32 DEG C, and fermentation time is 3 days, terminates hair
Ferment filters after being uniformly mixed into fermentation liquid by addition potassium dihydrogen phosphate, humic acid and urea, collects filtrate to get arriving
Biological organic matter；The fermentation liquid, potassium dihydrogen phosphate, humic acid and urea ratio be 1L:5g:3g:2g.
Embodiment 2
Influence of the different factors to production amount of threonine and yield of acetic acid in fermentation process of the present invention:
Group is set:
Experimental group: embodiment 1；
Control group 1: not adding Chlamydomonas reinhardtii, remaining is the same as embodiment 1；
Glycerol: being replaced with the glucose of equal quality by control group 2, remaining is the same as embodiment 1；
Control group 3: not adding Chlamydomonas reinhardtii, while glycerol being replaced with to the glucose of equal quality, remaining is the same as embodiment 1.
The content of threonine and acetic acid is shown in Table 1 in each final fermentation liquid of group:
Table 1
| [0001] group | [0002] threonine g/L | [0003] acetic acid g/L |
| [0004] experimental group | [0005] 127.9 | [0006] 0.7 |
| [0007] control group 1 | [0008] 103.5 | [0009] 12.6 |
| [0010] control group 2 | [0011] 115.7 | [0012] 4.9 |
| [0013] control group 3 | [0014] 97.1 | [0015] 14.8 |
Conclusion: experimental group can utilize the acetic acid in threonine fermentation liquid by carrying out assisted fermentation processing to Chlamydomonas reinhardtii
Non- light and effect are carried out as carbon source, to relieve the inhibiting effect that Escherichia coli are produced with threonine, additionally it is possible to carry out micro
Photosynthesis discharge oxygen, for Escherichia coli fermentation produce threonine come using；Part glucose is substituted by glycerol simultaneously, with
The consumption of glucose, Escherichia coli use glycerol as carbon source, due to cell absorb glycerol rate it is lower, reduce acetic acid
Accumulation, while improving the yield of threonine, pass through each group comparative test and find, the threonine of experimental group of the present invention produces
Highest is measured, and acetic acid content is minimum.
Embodiment 3
The manure trial of biological organic matter prepared by the present invention:
By taking tomato as an example, tomato yield and quality are detected.
Experimental group: sprinkling biological organic matter of the present invention；
Control group: sprinkling equivalent clear water.
The greenhouse in place selection Inner Mongol area；Every cell cultivated area is 10m × 10m, and 3 repetitions are arranged, set 6 altogether
A experimental plot, random district's groups arrangement.Tomato variety is powder benefit 1, sprays liquid fertilizer 100kg, other planting conditions per acre
It is identical.The results are shown in Table 2:
Table 2
| [0016] group | [0017] single fruit weight g | [0018] soluble solid % | [0019] soluble sugar % | [0020] titratable acid % |
| [0021] experimental group | [0022] 168.9 | [0023] 4.73 | [0024] 4.03 | [0025] 0.36 |
| [0026] control group | [0027] 162.4 | [0028] 4.55 | [0029] 3.89 | [0030] 0.41 |
Conclusion: as shown in table 2, compared with control group does not apply biological organic matter, the organic matter of experimental group can be improved single fruit weight,
The specific gravity that soluble solid and soluble sugar can also be improved reduces the specific gravity of titratable acid, improves Tomato Quality.
Embodiment 4
Egg white icing economic benefit situation analysis prepared by the present invention:
By taking our company produces 100000 tons of threonine workshops per year as an example, to after applying the present invention compared with traditional handicraft at one's duty
It analyses as follows:
By 2200 yuan/ton of mycoprotein price of separation and Extraction, annual 17000 tons of mycoprotein of sale, the costs such as dry are about
4000000 yuan, 37,400,000 yuan of income from sales, therefore 33,400,000 yuan of income can be increased newly every year；
It is about 12000 tons/year that protein hydrolysate cream, which is made, in above-mentioned thallus, is come according to presently commercially available 22000 yuan/ton of yeast extract unit price
It calculates, therefore can realize 264,000,000 yuan of income from sales, egg white icing per ton consumes enzyme preparation and 2500 yuan of processing cost, therefore every
Year can increase 234,000,000 yuan of income newly.
The above described is only a preferred embodiment of the present invention, be not intended to limit the present invention in any form, though
The right present invention has been described by way of example and in terms of the preferred embodiments, however, being not intended to limit the invention, any technology people for being familiar with this profession
Member can make a little change or modification using the technology contents disclosed certainly without departing from the scope of the present invention, at
For the equivalent embodiment of equivalent variations, but anything that does not depart from the technical scheme of the invention content, according to the technical essence of the invention
Any simple modification, equivalent change and modification to the above embodiments, belong in the range of technical solution of the present invention.
Claims (8)
1. handling the technique of threonine high gravity fermentation waste water comprising following steps: step 1) prepares threonine fermentation liquid, step
Rapid 2) to prepare egg white icing, step 3) prepares threonine, and step 4) collects Threonine Fermentation waste water, and step 5) prepares biological organic
Matter.
2. technique according to claim 1, described in feature, the step 1) prepares threonine fermentation liquid, including as follows
Step: threonine colibacillus engineering K12 △ dapA seed liquor will be produced and be linked into according to 8% inoculum concentration containing fermented and cultured
It ferments in the fermentor of base, 30 DEG C of temperature, tank pressure is 0.04MPa, ventilatory capacity 0.5vvm, revolving speed 100rpm, fermentation
Time is 36h, then accesses Chlamydomonas reinhardtii according to 10% inoculum concentration, continues fermented and cultured 36h, stop fermentation, collects threonine
Fermentation liquid.
3. technique according to claim 2, described in feature, the step 2 prepares egg white icing, includes the following steps: to revive
Propylhomoserin fermentation liquid first passes around disk plate centrifuge and is centrifuged 5min with 4000rpm, supernatant liquid and mycoprotein precipitating is collected, by bacterium
Body protein precipitating is added to reactor, and appropriate warm water is added and mixes well, and adjusts solid content 10%, adjusts enzymolysis temperature 55
DEG C, add a little sulfuric acid adjustment pH5.5, sequentially add lysozyme and acid protease, be slowly stirred, enzymolysis time 6h is used
Disk plate centrifuge is centrifuged 3min with 5000rpm, and supernatant is by separation removal cell wall through evaporator low-temperature evaporation to solid content
60%, egg white icing is made in the direct barrelling of gained paste.
4. technique according to claim 3, described in feature, the step 3) prepares threonine, includes the following steps:
Layer liquid passes through ceramic membrane filter, collects filtered solution and trapped substance, filtered solution is separated through decanter centrifuge, centrifugal speed is
5000rpm, centrifugation time 3min collect supernatant and sediment；Then pass through ultrafiltration membrance filter, collect filtered solution；It will filter
It crosses liquid and carries out condensing crystallizing, crystal and mother liquor is collected by centrifugation, it is 0.8% that crystal, which is then placed in 120 DEG C of dryings to moisture content,
Slabbing is compressed, then is put into granulation tower, is in fluidized state under the action of thermal current；65 DEG C of fluidized bed dryings, then through broken
Whole grain is to get threonine.
5. technique according to claim 4, described in feature, the step 4) collects Threonine Fermentation waste water, including such as
Lower step: trapped substance obtained by step 3), sediment and mother liquor are mixed, Threonine Fermentation waste water is obtained.
6. technique according to claim 5, described in feature, the step 5) prepares biological organic matter, including walks as follows
It is rapid: Threonine Fermentation waste water being heated to 100 DEG C, 10min is handled under heat-retaining condition, then cools to room temperature, ammonium hydroxide tune is added
Whole pH is 6.5, is then successively inoculated with bacillus subtilis and trichoderma viride carries out fermentation process, fermentation temperature is 32 DEG C, hair
The ferment time is 3 days, terminates fermentation, into fermentation liquid by addition potassium dihydrogen phosphate, humic acid and urea, mistake after being uniformly mixed
Filter collects filtrate to get biological organic matter is arrived.
7. technique according to claim 2, described in feature, the component of the fermentation medium are as follows: glucose 20g/L,
Glycerol 15g/L, corn pulp 15g/L, ammonium sulfate 2g/L, potassium dihydrogen phosphate 0.2g/L, dipotassium hydrogen phosphate 0.2g/L, epsom salt
0.1g/L, ferrous sulfate heptahydrate 0.01g/L, manganese sulfate monohydrate 0.01g/L, pH value 6.5.
8. technique according to claim 6, described in feature, in the step 5), fermentation liquid, potassium dihydrogen phosphate, humic
The ratio of acid and urea is 1L:5g:3g:2g.
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