CN109429911A - A kind of fermentation cordyceps sinensis culture solution and preparation method thereof prepared with jerusalem artichoke - Google Patents
A kind of fermentation cordyceps sinensis culture solution and preparation method thereof prepared with jerusalem artichoke Download PDFInfo
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- CN109429911A CN109429911A CN201811380238.6A CN201811380238A CN109429911A CN 109429911 A CN109429911 A CN 109429911A CN 201811380238 A CN201811380238 A CN 201811380238A CN 109429911 A CN109429911 A CN 109429911A
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- jerusalem artichoke
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to technical field of fungal fermentation, and in particular to a kind of fermentation cordyceps sinensis culture solution and preparation method thereof prepared with jerusalem artichoke, and the method using culture medium production aweto strain and aweto mycelium.The culture solution includes following components: fresh 200~300g/L of jerusalem artichoke juice or dry 40~60g/L of jerusalem artichoke, 20~30g/L of glucose, 15~25g/L of yeast extract, 0.1~0.5g/L of magnesium sulfate, 0.1~0.5g/L of potassium dihydrogen phosphate.The advantages of fermented wine provided by the invention: (1) raw material composition has been lacked in letter, simplifies the preparation process of culture solution, improves the controllability and stability of technique.(2) raw material sources are extensive, price steadiness, reduce costs.(3) without animal virulence factor risk, content of beary metal is low, antibiotic-free residual, highly-safe.(4) the mycelium fermentation period is short, microbiological contamination risk is low, low energy consumption.(5) mycelial yield is high, good in economic efficiency.(6) stable product quality meets relevant criterion.
Description
Technical field
The present invention relates to technical field of fungal fermentation, and in particular to a kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke
And preparation method thereof, and utilize the method for culture medium production aweto strain and aweto mycelium.
Background technique
Because wild resource is exhausted year by year, the protection of resources and sustainable use of cordyceps sinensis are always to close extensively inside and outside industry
The Chinese caterpillar fungus bacterial filament powder of the focus of note, the production of artificial fermentation's method has been known and has approved.
Anamorph of Cordyceps Sinensis strain Hirsutella sinensis (Hirsutella sinensis Lu, Guo, Yu et Zeng),
Many personal and unit researchs domestic at present and open Chinese caterpillar fungus culture medium and fermentation method for producing.In the prior art, Chinese
It is substantially trained by liquid fermentation of the supplementary material of the animal sources such as degreasing silkworm chrysalis, ox bone peptone, fish peptone in the production of coat spore
Feeding primary raw material, the scarcity of silkworm chrysalis and the monopolization of supply cause its price to rise year by year, and the promotion of ox bone price also causes
The price of ox bone peptone goes up year by year, this is also to lead to high one of the reason of Chinese caterpillar fungus bacterium powder price of fermenting.In addition, due to
Silkworm chrysalis has the distinctive fishy smell of animal protein, and the mouthfeel for extracting mycelia post-fermentation liquid to culture solution has a significant impact.Furthermore at present
There are the following problems: the risks such as animal virulence factor, rabid ox disease for fermenting and producing for animal protein peptone；Content of beary metal is exceeded；
Antibiotic residual quantity is high.Although not including in fermented aweto hypha product Physico-chemical tests index as defined in Chinese Pharmacopoeia above-mentioned
Index, but security risk is still very important problem.
Summary of the invention
The purpose of the present invention is overcoming the deficiencies of existing technologies, one kind is provided and is able to produce high-quality, highly-safe, effect
With nutrient composition content height, the culture solution without fishy smell and lower-cost aweto mycelium.
In order to achieve the above objectives, the technology that the present invention uses solves are as follows:
It is a kind of with jerusalem artichoke prepare fermentation cordyceps sinensis culture solution, include following components: fresh 200~300g/L of jerusalem artichoke juice or
Dry 40~60g/L of jerusalem artichoke, 20~30g/L of glucose, 15~25g/L of yeast extract, 0.1~0.5g/L of magnesium sulfate, potassium dihydrogen phosphate
0.1~0.5g/L.
Further, the fresh jerusalem artichoke juice be the full juice of jerusalem artichoke, by after cleaning fresh jerusalem artichoke stem tuber or/and jerusalem artichoke cauline leaf break
Broken, homogeneous preparation.
Further, the jerusalem artichoke is dry by the dry gained of fresh jerusalem artichoke system, and when use is crushed to 20~80 mesh.
Further, the yeast extract is Saccharomyces cerevisiae cream.
Further, 5~15g/L agar is also contained in the culture medium.
The present invention also provides a kind of preparation methods of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke.
The culture medium the preparation method comprises the following steps:
Dissolution: fresh jerusalem artichoke juice, yeast extract and 28~35 DEG C of water, stirring and dissolving is added by formula and obtains mixed liquor；Or by matching
Dry jerusalem artichoke and water is added in side, is heated to 100~105 DEG C, Temperature fall 4~8 hours, takes supernatant that must liquefy feed liquid, liquefaction material
Yeast extract is added in liquid, is uniformly mixed to obtain mixed liquor；
It prepares: glucose, magnesium sulfate and potassium dihydrogen phosphate is added by formula in mixed liquor, with sodium hydroxide tune PH to 7.2
~7.5 to get the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke.
Further, the preparation method of the culture medium further include: 5~15g/L agar is added after preparation.
The present invention also provides a kind of sides of fermentation cordyceps sinensis culture solution production aweto mycelium prepared with jerusalem artichoke
Method.
This method comprises:
Addition plant oil defoaming agent in the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke, 0.11~
0.15MPa, 121~125 DEG C, sterilize 30~60min, is cooled to room temperature；
Filtrated air, 0.11~0.16MPa of ventilatory capacity, 13~18 DEG C of cultures, until fermentation liquid reduced sugar is dense are passed through after inoculation
It spends < 12g/L or fermentation liquid amino nitrogen < 0.2g/L and stops fermentation, separate mycelia, it is dry to get aweto mycelium.
Further, the temperature of the filtrated air is greater than real-time air themperature.
The present invention also provides a kind of fermentation cordyceps sinensis culture solutions prepared with jerusalem artichoke to produce aweto strain method.
This method comprises: agar is added in the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke, it is heated to agar
It dissolves；0.11~0.15MPa, 121~125 DEG C, sterilize 30~60min, is cooled to 40~85 DEG C, and inclined-plane is put in packing to test tube,
It is cooled to room temperature；Inoculation, 13~18 DEG C are cultivated 30~45 days, obtain cordyceps sinensis inclined-plane solid spawn.
Cordyceps sinensis culture solution provided by the invention replaces the carbon sources class raw material such as corn and wheat bran with jerusalem artichoke, with reduce at
This；Meanwhile jerusalem artichoke replaces silkworm chrysalis and original animal derived protein peptone (meat peptone, Fish protein as nitrogen source and yeast extract together
Peptone, bone protein peptone, fish peptone etc.) etc. nitrogen sources, provide that a kind of raw material sources are more extensive, and economic cost is lower, safety is more preferable
Whole plant source culture medium and production technology.
Fermentation cordyceps sinensis culture solution has been lacked in the fermentation cordyceps sinensis culture solution provided by the invention prepared with jerusalem artichoke, letter
Raw material composition, is reduced to 5 kinds by original at least eight kinds of raw materials, simplifies the preparation process of culture solution, improve the controllable of technique
Property and stability.
Fresh jerusalem artichoke contains albumen quality 6.3~8.7%, and total reducing sugar 73~87% belongs to the microculture of carbon nitrogen double-purpose
Based raw material, in addition, jerusalem artichoke has the function of increment mushroom breeding.Synanthrin also has control blood lipid, reduces the danger of cardiovascular disease
Evil reduces ammonia concentration, promotes the absorption of minerals, anti-to treat constipation and other effects, is suitable for diabetic.Synanthrin can also be adjusted
Metabolic function is saved, lipid-metabolism rate, strengthen immunity are improved and improves mineral absorption conversion.Therefore, not only using jerusalem artichoke
It is able to satisfy the production requirement of aweto, moreover it is possible to provide additional health-care efficacy.
Jerusalem artichoke is the crops of a kind of no disease pest harm and pollution by pesticides, and yield is high, single currently with approach, uses chrysanthemum
Taro replaces corn and wheat bran, can not only reduce cost, and its safety is higher.
Jerusalem artichoke distribution is wide, has cultivation in Chinese north and south various regions；Adaptable, impoverishment tolerant resists cold, drought-enduring；Plantation is simple,
Primary sowing Multiple harvests；Yield is high.Therefore, source and price steadiness, it is at low cost.
The fermentation cordyceps sinensis culture solution provided by the invention prepared with jerusalem artichoke, is replaced with vegetable raw material and microorganism raw material
For animal raw materials, antibiotic is not used in production process, therefore antibiotic-free remains.There will not be animal virulence factor wind
Danger.Plant belongs to the producer in the ecosystem, although meeting enriching heavy metal, compared to the animal of consumer, the weight of enrichment
Metal is far smaller than the heavy metal of animal enrichment.
Vegetable raw material is lower compared with animal raw materials production cost, and source is more stable, and quality control is simpler.Therefore, originally
Invention provide with jerusalem artichoke prepare fermentation cordyceps sinensis culture solution and using the culture solution production aweto strain and
Mycelium is at low cost, high-quality, highly-safe, effect and nutrient composition content are high.
Aweto mycelium is produced using culture medium provided by the invention, shortens fermentation period, it can be by original total week
It shortened within phase 36-40 days 30~36 days, total fermentation period shortening can reduce microbiological contamination risk, and reduce consumption.It can also improve simultaneously
Mycelial yield, yield increase to 12.98 ‰ by original 9 ‰ or so, and standards of pharmacopoeia is also fully achieved in every quality index of product
Requirement.
Compared with prior art, it is provided by the invention with jerusalem artichoke prepare fermentation cordyceps sinensis culture solution and utilize the culture
Liquid produces the advantages of aweto strain and mycelial method:
(1) raw material composition has been lacked in letter, simplifies the preparation process of culture solution, improves the controllability and stability of technique.
(2) raw material sources are extensive, price steadiness, reduce costs.
(3) without animal virulence factor risk, content of beary metal is low, antibiotic-free residual, highly-safe.
(4) the mycelium fermentation period is short, microbiological contamination risk is low, low energy consumption.
(5) mycelial yield is high, good in economic efficiency.
(6) stable product quality meets relevant criterion.
Specific embodiment
It is a kind of with jerusalem artichoke prepare fermentation cordyceps sinensis culture solution, include following components: fresh 200~300g/L of jerusalem artichoke juice or
Dry 40~60g/L of jerusalem artichoke, 20~30g/L of glucose, 15~25g/L of yeast extract, 0.1~0.5g/L of magnesium sulfate, potassium dihydrogen phosphate
0.1~0.5g/L.
The fresh jerusalem artichoke juice is the full juice of jerusalem artichoke, by after cleaning fresh jerusalem artichoke stem tuber or/and jerusalem artichoke cauline leaf is broken, homogeneous system
It is standby.
The jerusalem artichoke is dry by the dry gained of fresh jerusalem artichoke system, and when use is crushed to 20~80 mesh.
The yeast extract is Saccharomyces cerevisiae cream.
Also contain 5~15g/L agar in the culture medium.
The present invention also provides a kind of preparation methods of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke.
The culture medium the preparation method comprises the following steps:
Dissolution: fresh jerusalem artichoke juice, yeast extract and 28~35 DEG C of water, stirring and dissolving is added by formula and obtains mixed liquor；Or by matching
Dry jerusalem artichoke and water is added in side, is heated to 100~105 DEG C, Temperature fall 4~8 hours, takes supernatant that must liquefy feed liquid, liquefaction material
Yeast extract is added in liquid, is uniformly mixed to obtain mixed liquor；
It prepares: glucose, magnesium sulfate and potassium dihydrogen phosphate is added by formula in mixed liquor, with sodium hydroxide tune PH to 7.2
~7.5 to get the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke.
The preparation method of the culture medium further include: 5~15g/L agar is added after preparation.
The present invention also provides a kind of sides of fermentation cordyceps sinensis culture solution production aweto mycelium prepared with jerusalem artichoke
Method.
This method comprises:
Addition plant oil defoaming agent in the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke, 0.11~
0.15MPa, 121~125 DEG C, sterilize 30~60min, is cooled to room temperature；
Filtrated air, 0.11~0.16MPa of ventilatory capacity, 13~18 DEG C of cultures, until fermentation liquid reduced sugar is dense are passed through after inoculation
It spends < 12g/L or fermentation liquid amino nitrogen < 0.2g/L and stops fermentation, separate mycelia, it is dry to get aweto mycelium.
The temperature of the filtrated air is greater than real-time air themperature.
The present invention also provides a kind of fermentation cordyceps sinensis culture solutions prepared with jerusalem artichoke to produce aweto strain method.
This method comprises: agar is added in the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke, it is heated to agar
It dissolves；0.11~0.15MPa, 121~125 DEG C, sterilize 30~60min, is cooled to 40~85 DEG C, and inclined-plane is put in packing to test tube,
It is cooled to room temperature；Inoculation, 13~18 DEG C are cultivated 30~45 days, obtain cordyceps sinensis inclined-plane solid spawn.
Embodiment 1
A kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke includes following components: fresh jerusalem artichoke juice 200g/L, glucose
30g/L, yeast extract 25g/L, magnesium sulfate 0.5g/L, potassium dihydrogen phosphate 0.5g/L.
Embodiment 2
A kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke includes following components: fresh jerusalem artichoke juice 300g/L, glucose
20g/L, yeast extract 15g/L, magnesium sulfate 0.1g/L, potassium dihydrogen phosphate 0.1g/L.
Embodiment 3
A kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke includes following components: dry jerusalem artichoke 50g/L, glucose
25g/L, yeast extract 20g/L, magnesium sulfate 0.25g/L, potassium dihydrogen phosphate 0.25g/L.
Embodiment 4
A kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke includes following components: dry jerusalem artichoke 40g/L, glucose
25g/L, yeast extract 20g/L, magnesium sulfate 0.2g/L, potassium dihydrogen phosphate 0.3g/L, agar 10g/L.
Embodiment 5
With jerusalem artichoke prepare fermentation cordyceps sinensis culture solution preparation method:
Dissolution: fresh jerusalem artichoke juice, yeast extract and 28 DEG C of water, stirring and dissolving is added by the formula of embodiment 1 and obtains mixed liquor；
Prepare: glucose, magnesium sulfate and the potassium dihydrogen phosphate being added in mixed liquor by the formula of embodiment 1 use sodium hydroxide
Adjust PH to 7.2 to get the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke.
Embodiment 6
With jerusalem artichoke prepare fermentation cordyceps sinensis culture solution preparation method:
Dissolution: dry jerusalem artichoke juice and water is added by the formula of embodiment 3, is heated to 102 DEG C, Temperature fall 6 hours, takes supernatant
Must liquefy feed liquid, and yeast extract is added in the feed liquid that liquefies, and be uniformly mixed to obtain mixed liquor；
It prepares: glucose, magnesium sulfate and potassium dihydrogen phosphate is added by the formula of embodiment 3 in mixed liquor, with sodium hydroxide tune
PH to 7.3 is to get the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke.
Embodiment 7
Aweto strain method is produced with fermentation cordyceps sinensis culture solution prepared by jerusalem artichoke:
Dissolution: fresh jerusalem artichoke juice, yeast extract and 35 DEG C of water, stirring and dissolving is added by the formula of embodiment 2 and obtains mixed liquor；
It prepares: glucose, magnesium sulfate and potassium dihydrogen phosphate is added by by the formula of embodiment 2 in mixed liquor, uses sodium hydroxide
PH to 7.5 is adjusted, 5~15g/L agar is added, heating and melting is to get the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke.
Embodiment 8
The method that the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke produces aweto mycelium:
Plant oil defoaming agent is added in 5 culture solution of embodiment, 0.11MPa, 121 DEG C, sterilize 60min, is cooled to room temperature；
It is passed through filtrated air after inoculation, the temperature of filtrated air is greater than real-time air themperature, ventilatory capacity 0.15MPa, and 16 DEG C
Culture separates mycelia until fermentation liquid concentration of reduced sugar 12g/L stops fermentation, dry to get aweto mycelium.
Embodiment 9
Aweto strain method is produced with fermentation cordyceps sinensis culture solution prepared by jerusalem artichoke:
7 culture solution 0.15MPa of embodiment, 125 DEG C, sterilize 30min, is cooled to 40 DEG C, and inclined-plane is put in packing to test tube, cold
But to room temperature；Inoculation, 18 DEG C are cultivated 30 days, obtain cordyceps sinensis inclined-plane solid spawn.
Above embodiments describe the prefered embodiments of the present invention in detail, and still, the present invention is not limited to above-mentioned implementations
Detail in mode can carry out technical solution of the present invention a variety of simple within the scope of the technical concept of the present invention
Modification, these simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (10)
1. a kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke, which is characterized in that include following components: fresh jerusalem artichoke juice 200
~300g/L or dry 40~60g/L of jerusalem artichoke, 20~30g/L of glucose, 15~25g/L of yeast extract, 0.1~0.5g/L of magnesium sulfate,
0.1~0.5g/L of potassium dihydrogen phosphate.
2. a kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke according to claim 1, it is characterised in that: described
Fresh jerusalem artichoke juice is the full juice of jerusalem artichoke, by after cleaning fresh jerusalem artichoke stem tuber or/and jerusalem artichoke cauline leaf is broken, prepared by homogeneous.
3. a kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke according to claim 1, it is characterised in that: described
Jerusalem artichoke is dry by the dry gained of fresh jerusalem artichoke system, and when use is crushed to 20~80 mesh.
4. a kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke according to claim 1, it is characterised in that: described
Yeast extract is Saccharomyces cerevisiae cream.
5. a kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke according to any one of claims 1 to 3, feature
It is: also contains 5~15g/L agar in the culture medium.
6. a kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke according to any one of claims 1 to 3, feature
Be: the culture medium the preparation method comprises the following steps:
Dissolution: fresh jerusalem artichoke juice, yeast extract and 28~35 DEG C of water, stirring and dissolving is added by formula and obtains mixed liquor；Or add by formula
Enter dry jerusalem artichoke and water, be heated to 100~105 DEG C, Temperature fall 4~8 hours, takes supernatant that must liquefy feed liquid, in the feed liquid that liquefies
Yeast extract is added, is uniformly mixed to obtain mixed liquor；
Prepare: glucose, magnesium sulfate and potassium dihydrogen phosphate be added by formula in mixed liquor, with sodium hydroxide tune PH to 7.2~
7.5 to get the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke.
7. a kind of raw using a kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke described in 3 any one of claims 1 to 3
The method for producing aweto mycelium, which is characterized in that this method comprises:
Addition plant oil defoaming agent in the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke, 0.11~0.15MPa,
121~125 DEG C, sterilize 30~60min, is cooled to room temperature；
Filtrated air, 0.11~0.16MPa of ventilatory capacity, 13~18 DEG C of cultures, until fermentation liquid concentration of reduced sugar < are passed through after inoculation
12g/L or fermentation liquid amino nitrogen < 0.2g/L stops fermentation, separates mycelia, dry to get aweto mycelium.
8. a kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke according to claim 5, it is characterised in that: described
The preparation method of culture medium further include: 5~15g/L agar is added after preparation.
9. a kind of produce aweto using a kind of fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke as claimed in claim 4
Kind method, which is characterized in that this method comprises: agar is added in the fermentation cordyceps sinensis culture solution prepared with jerusalem artichoke,
Agar is heated to dissolve；0.11~0.15MPa, 121~125 DEG C, sterilize 30~60min, is cooled to 40~85 DEG C, packing to examination
Pipe is put inclined-plane, is cooled to room temperature；Inoculation, 13~18 DEG C are cultivated 30~45 days, obtain cordyceps sinensis inclined-plane solid spawn.
10. the fermentation cordyceps sinensis culture solution production aweto mycelium according to claim 6 prepared with jerusalem artichoke
Method, it is characterised in that: the temperature of the filtrated air is greater than real-time air themperature.
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Application publication date: 20190308 |