CN109234141B - Be provided with micro-fluidic structure's biological reaction device - Google Patents
Be provided with micro-fluidic structure's biological reaction device Download PDFInfo
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- CN109234141B CN109234141B CN201810440562.6A CN201810440562A CN109234141B CN 109234141 B CN109234141 B CN 109234141B CN 201810440562 A CN201810440562 A CN 201810440562A CN 109234141 B CN109234141 B CN 109234141B
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Abstract
The invention discloses a biological reaction device with a microfluidic structure, which comprises an extraction bin, a reaction bin and the microfluidic structure for transferring a macromolecular extract in the extraction bin to the reaction bin; a pneumatic pump is arranged above the micro-fluidic structure, and a gas guide channel for communicating the extraction bin with the pneumatic pump and an extracting solution channel for communicating the extraction bin with the reaction bin are arranged in the micro-fluidic structure; one end of the extracting solution channel is communicated with a liquid conduction suction head arranged in the extracting bin, and the other end of the extracting solution channel is communicated with a liquid guide pipe arranged in the reaction bin. The biological reaction device with the micro-fluidic structure is provided with the micro-fluidic structure to communicate the extraction bin and the reaction bin, and extracts materials (protein and nucleic acid) are fully and efficiently transferred from the extraction bin to the reaction bin through the micro-fluidic structure, so that the experimental process is simplified, the experimental efficiency is improved, the possibility of pollution during sample transfer is reduced, the biological reaction device is simple in structure and convenient to operate, and the complexity of equipment matched with the biological reaction device can be effectively reduced.
Description
Technical Field
The invention relates to the technical field of biochemistry and molecular biology, in particular to a biological reaction device with a microfluidic structure.
Background
With the change of medical treatment mode and the continuous development of individual medication, the medical inspection world needs a fast and accurate detection means, and the molecular detection exerts unique advantages. The basis of molecular detection is to analyze the tissue cells, hair, anticoagulation blood or dry blood traces of a detected person, genes and expression products thereof in formaldehyde-fixed paraffin-embedded tissues, and complete nucleic acid (DNA and RNA) detection from a molecular level, so that the detection can be accurately made before symptoms, signs and biochemical changes even do not appear once the disease occurs.
At present, the molecular detection technology mainly comprises nucleic acid molecular hybridization, Polymerase Chain Reaction (PCR), biochip technology and the like. The molecular detection product is mainly applied to detection of clinical departments such as tumor, infection, heredity, prenatal screening and the like, and aspects such as a physical examination center, a technical service center, a third-party detection mechanism, a microorganism rapid detection market and the like. At present, the examination means such as blood routine, cytology, pathology and immunology are developed towards automation, integration and standardization, but due to the technical complexity of molecular detection, the platform of a full-automatic instrument from sample to result is few or has a plurality of technical problems which are difficult to solve. For example, in the field of biotechnology, nucleic acid extraction by a centrifugal column method or a magnetic bead method generally requires four steps of lysis, binding, rinsing, elution, and the like, and further includes detection steps of nucleic acid molecular hybridization, Polymerase Chain Reaction (PCR), a biochip, and the like, so that it is very difficult to realize a full-automatic instrument from a sample to a result. Moreover, the whole operation process is easy to cause pollution, the purity of the extract is influenced, and the materials are difficult to be transferred fully and efficiently, so that the experimental result is influenced. Therefore, in view of the technical complexity of molecular detection, the "full-automatic instrument platform from sample to result" has few or many technical problems that are difficult to solve, and to realize sufficient and efficient transfer of materials between each step, it is necessary to consider various aspects of the structure and function of consumables and experimental equipment, so as to avoid the problems of high cost, poor sensitivity, complex structure, too complex operation and related detection equipment in the prior art.
Disclosure of Invention
Aiming at the defects in the prior art, the technical problem to be solved by the invention is to provide a biological reaction device with a microfluidic structure, wherein the microfluidic structure is arranged to communicate an extraction bin and a reaction bin, and extracted materials (protein and nucleic acid) are fully and efficiently transferred from the extraction bin to the reaction bin through the microfluidic structure, so that the experimental process is simplified, the experimental efficiency is improved, and the possibility of pollution during sample transfer is reduced.
The invention also provides a biological reaction device with a microfluidic structure, which has simple structure and convenient operation and can effectively reduce the complexity of equipment matched with the biological reaction device.
In order to solve the technical problem, the biological reaction device provided with the microfluidic structure provided by the embodiment of the invention comprises an extraction bin, a reaction bin and the microfluidic structure for transferring a macromolecular extract in the extraction bin to the reaction bin;
the mixing driver is fixed on the micro-fluidic structure, a mixing spray head penetrating through the micro-fluidic structure and inserted below the liquid level in the extraction bin is arranged on the mixing driver, and materials in the extraction bin can be mixed by pushing and pulling the mixing driver;
a pneumatic pump is further arranged above the micro-fluidic structure, and a gas guide channel for communicating the extraction bin with the pneumatic pump and an extracting solution channel for communicating the extraction bin with the reaction bin are arranged in the micro-fluidic structure; one end of the extracting solution channel is communicated with a liquid conduction suction head arranged in the extracting bin, and the other end of the extracting solution channel is communicated with a liquid guide pipe arranged in the reaction bin;
after the pneumatic pump is pressed, pressure gas in the pneumatic pump enters the extraction bin through the gas guide channel, and under the action of the pressure gas, an extracting solution in the extraction bin passes through the liquid conduction suction head and enters the reaction bin through the extracting solution channel and the liquid guide pipe;
a reaction liquid storage cavity is further arranged above the micro-fluidic structure, a reaction liquid injection channel is further arranged in the micro-fluidic structure, and the reaction liquid injection channel is communicated with the reaction bin through a reaction liquid injection guide pipe arranged above the reaction bin; before the reaction chamber reacts, the reaction liquid in the reaction liquid storage cavity passes through the reaction liquid injection channel and is injected into the reaction chamber through the reaction liquid injection conduit, and the reaction liquid is mixed with the extracting solution which is obtained from the extracting chamber and transferred into the reaction chamber to react.
As a preferred aspect of the present invention, the biological reaction device provided with the microfluidic structure provided in the embodiments of the present invention further includes some or all of the following technical features:
preferably, a liquid valve capable of blocking or opening the extracting solution channel is further arranged above the microfluidic structure. Preferably, the valve is a press type valve, after a valve button on the valve is pressed, a valve channel is communicated with the extracting solution channel, and the extracting solution channel is opened; after the valve button on the valve is pulled up, the valve channel deviates from the extracting solution channel, and the extracting solution channel is closed.
Preferably, the extraction chamber contains magnetic beads to which an extract of macromolecules including nucleic acids or proteins is adsorbed.
Preferably, an electromagnet for adsorbing magnetic beads in the extraction bin is arranged outside the extraction bin, and the electromagnet is arranged on one side, far away from the liquid conduction suction head, outside the extraction bin; when the materials in the mixed extraction bin are pushed and pulled so as to effectively elute the extract adsorbed on the magnetic beads in the extraction bin, the electromagnet is in a non-electrified state, and the magnetic beads are dispersed in the extraction bin; when the extract needs to be transferred from the extraction bin to the reaction bin for effective elution, the electromagnet is in a powered state, and the magnetic beads are adsorbed on the side away from the liquid conduction suction head.
Preferably, the reaction liquid storage chamber is provided with one or more than two. More preferably, in one embodiment of the present invention, the reaction liquid storage chambers are provided in two.
Preferably, the reaction liquid storage cavity comprises a piston type push-pull structure, and the piston type push-pull structure is provided with a puncture structure; a cavity for containing the reaction liquid is formed in the reaction liquid storage cavity, and the bottom of the cavity is provided with a packaging film; when the reaction liquid needs to be injected into the reaction bin, the piston type push-pull structure is pushed, the puncture structure pierces the packaging film at the bottom of the cavity, and the reaction liquid passes through the reaction liquid injection channel and is injected into the reaction bin through the reaction liquid guide pipe.
Preferably, the extraction bin and the reaction bin are both detachable structures.
Preferably, the extraction chamber and the reaction chamber are fixed to the main body structure of the biological reaction device provided with the microfluidic structure by means of a buckle or a thread.
The biological reaction device provided with the microfluidic structure provided by the embodiment of the invention has the following beneficial effects:
the biological reaction device provided with the micro-fluidic structure is communicated with the extraction bin and the reaction bin by the micro-fluidic structure, and extracted materials (protein and nucleic acid) are fully and efficiently transferred from the extraction bin to the reaction bin by the micro-fluidic structure, so that the experimental process is simplified, the experimental efficiency is improved, and the possibility of pollution during sample transfer is reduced. Specifically, the biological reaction device with the microfluidic structure is provided with the mixing driver, and materials in the extraction bin are mixed and disturbed by pushing and pulling the mixing driver, so that the extracts adsorbed on the magnetic beads are sufficiently eluted; in addition, through being provided with the pneumatic pump, after being exerted pressure to this pneumatic pump, the pressure gas in the pneumatic pump gets into and draws the storehouse through the gas guide passageway, under the pressure gas effect, draws the extract in the storehouse and passes through liquid conduction suction head and get into in the reaction storehouse via extract passageway and liquid conduit to realize fully, high-efficiently transferring the extraction material (protein, nucleic acid) from drawing the storehouse to the reaction storehouse, simplify the experimental procedure and improve experimental efficiency and reduce the contaminated possibility when the sample shifts.
In addition, from the overall structure, the biological reaction device provided with the microfluidic structure has a simple structure and is convenient to operate, the structures of the microfluidic structure, the pressing type valve, the detachable extraction bin and reaction bin, the piston type reaction liquid storage cavity, the mixing driver, the pneumatic pump and the like which are arranged on the biological reaction device can be well matched with an external automatic device, particularly a pressing or pushing device with an air cylinder or mechanically pushed, and related external equipment which realizes simple actions such as the like can be greatly simplified, so that the complexity of equipment matched with the biological reaction device is effectively reduced.
Drawings
Fig. 1 is a first schematic structural diagram of a biological reaction device provided with a microfluidic structure according to a preferred embodiment of the present invention.
Fig. 2 is a schematic structural diagram ii of a biological reaction device provided with a microfluidic structure according to a preferred embodiment of the present invention.
Fig. 3 is a schematic structural view of a microfluidic structure in a bioreactor provided with the microfluidic structure according to a preferred embodiment of the present invention.
Fig. 4 is a partially enlarged view of fig. 2 in a region a.
Detailed Description
The embodiment of the invention provides a biological reaction device with a microfluidic structure, which comprises an extraction bin, a reaction bin and the microfluidic structure for transferring a macromolecular extract in the extraction bin to the reaction bin; the mixing driver is fixed on the micro-fluidic structure, a mixing spray head penetrating through the micro-fluidic structure and inserted below the liquid level in the extraction bin is arranged on the mixing driver, and materials in the extraction bin can be mixed by pushing and pulling the mixing driver; a pneumatic pump is further arranged above the micro-fluidic structure, and a gas guide channel for communicating the extraction bin with the pneumatic pump and an extracting solution channel for communicating the extraction bin with the reaction bin are arranged in the micro-fluidic structure; one end of the extracting solution channel is communicated with a liquid conduction suction head arranged in the extracting bin, and the other end of the extracting solution channel is communicated with a liquid guide pipe arranged in the reaction bin; after the pneumatic pump is pressed, pressure gas in the pneumatic pump enters the extraction bin through the gas guide channel, and under the action of the pressure gas, an extracting solution in the extraction bin passes through the liquid conduction suction head and enters the reaction bin through the extracting solution channel and the liquid guide pipe; a reaction liquid storage cavity is further arranged above the micro-fluidic structure, a reaction liquid injection channel is further arranged in the micro-fluidic structure, and the reaction liquid injection channel is communicated with the reaction bin through a reaction liquid injection guide pipe arranged above the reaction bin; before the reaction chamber reacts, the reaction liquid in the reaction liquid storage cavity passes through the reaction liquid injection channel and is injected into the reaction chamber through the reaction liquid injection conduit, and the reaction liquid is mixed with the extracting solution which is obtained from the extracting chamber and transferred into the reaction chamber to react.
And a liquid valve capable of blocking or opening the extracting solution channel is also arranged above the microfluidic structure. In a preferred embodiment of the present invention, the valve is a press type valve, and after a valve button on the valve is pressed once, the valve channel is communicated with the extracting solution channel, and the extracting solution channel is opened; and the valve channel deviates from the extracting solution channel after the valve button on the valve is pressed again, and the extracting solution channel is closed.
Wherein, the extraction bin contains magnetic beads which adsorb the extracts of macromolecules including nucleic acid or protein.
Wherein, an electromagnet for adsorbing magnetic beads in the extraction bin is also arranged outside the extraction bin, and the electromagnet is arranged on one side of the extraction bin, which is far away from the liquid conduction sucker; when the materials in the mixed extraction bin are pushed and pulled so as to effectively elute the extract adsorbed on the magnetic beads in the extraction bin, the electromagnet is in a non-electrified state, and the magnetic beads are dispersed in the extraction bin; when the extract needs to be transferred from the extraction bin to the reaction bin for effective elution, the electromagnet is in a powered state, and the magnetic beads are adsorbed on the side away from the liquid conduction suction head.
Wherein, the reaction liquid storage cavity is provided with one or more than two. In a preferred embodiment of the present invention, the reaction liquid storage chambers are provided in two in one embodiment of the present invention.
The reaction liquid storage cavity comprises a piston type push-pull structure, and the piston type push-pull structure is provided with a puncture structure; a cavity for containing the reaction liquid is formed in the reaction liquid storage cavity, and the bottom of the cavity is provided with a packaging film; when the reaction liquid needs to be injected into the reaction bin, the piston type push-pull structure is pushed, the puncture structure pierces the packaging film at the bottom of the cavity, and the reaction liquid passes through the reaction liquid injection channel and is injected into the reaction bin through the reaction liquid guide pipe.
Wherein, draw storehouse and reaction storehouse and be the dismantlement formula structure.
The extraction bin and the reaction bin are fixed on a main body structure of the biological reaction device provided with the microfluidic structure in a buckling or threaded mode.
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Fig. 1 and 2 are schematic structural views of a bioreactor provided with a microfluidic structure according to a preferred embodiment of the present invention. The biological reaction device with the micro-fluidic structure is applied to nucleic acid or protein preparation and detection equipment, aims to provide an efficient and quick solution for the acquisition of nucleic acid or protein samples and the transfer of materials between adjacent steps in the subsequent detection process, and is not limited by the above.
As shown in fig. 1 to 4, the biological reaction apparatus provided with the microfluidic structure 30 according to the preferred embodiment of the present invention includes an extraction chamber 10, a reaction chamber 20, and a microfluidic structure 30 for transferring a macromolecular extract in the extraction chamber 10 to the reaction chamber 20; a mixing driver 40 is arranged above the micro-fluidic structure 30, the mixing driver 40 is fixed on the micro-fluidic structure 30, a mixing nozzle 41 which penetrates through the micro-fluidic structure 30 and is inserted into the extraction bin 10 below the liquid level is arranged on the mixing driver 40, and materials in the extraction bin 10 can be mixed by pushing and pulling the mixing driver 40; a pneumatic pump 50 is also arranged above the micro-fluidic structure 30, and an air guide channel 31 for communicating the extraction bin 10 with the pneumatic pump 50 and an extracting solution channel 32 for communicating the extraction bin 10 with the reaction bin 20 are arranged in the micro-fluidic structure 30; one end of the extracting solution channel 32 is communicated with a liquid conduction suction head 51 arranged in the extracting bin 10, and the other end of the extracting solution channel 32 is communicated with a liquid conduit 21 arranged in the reaction bin 20; after the pneumatic pump 50 is pressed, the pressure gas in the pneumatic pump 50 enters the extraction bin 10 through the gas guide channel 31, and under the action of the pressure gas, the extracting solution in the extraction bin 10 passes through the liquid conduction suction head 51 and enters the reaction bin 20 through the extracting solution channel 32 and the liquid guide pipe 21; a reaction liquid storage cavity 70 is further arranged above the microfluidic structure 30, a reaction liquid injection channel 33 is further arranged in the microfluidic structure 30, and the reaction liquid injection channel 33 is communicated with the reaction bin 20 through a reaction liquid injection conduit 22 arranged above the reaction bin 20; before the reaction chamber 20 reacts, the reaction solution in the reaction solution storage chamber 70 is injected into the reaction chamber 20 through the reaction solution injection channel 33 and the reaction solution injection conduit 22, and is mixed with the extraction solution obtained from the extraction chamber 10 and transferred into the reaction chamber 20 to react.
In a preferred embodiment of the present invention, a liquid valve 60 capable of blocking or opening the extracting liquid channel 32 is further disposed above the microfluidic structure 30. In the concrete implementation, the valve 60 is a press type valve 60, after a valve button 61 on the valve 60 is pressed, a valve channel 62 is communicated with the extracting solution channel 32, and the extracting solution channel 32 is opened; after pulling the valve button 61 on the valve 60, the valve passage 62 is deviated from the extracting fluid passage 32, and the extracting fluid passage 32 is closed.
In a preferred embodiment of the present invention, the extraction cartridge 10 contains magnetic beads having adsorbed thereon an extract of macromolecules including nucleic acids or proteins.
In addition, an electromagnet for adsorbing magnetic beads in the extraction bin 10 is arranged outside the extraction bin 10, and the electromagnet is arranged on one side, far away from the liquid conduction sucker 51, outside the extraction bin 10; when the materials in the extraction bin 10 are mixed by pushing and pulling the mixing driver 40 so as to effectively elute the extract adsorbed on the magnetic beads in the extraction bin 10, the electromagnet is in a non-energized state, and the magnetic beads are dispersed in the extraction bin 10; when the extraction solution is transferred from the extraction chamber 10 to the reaction chamber 20 for effective elution of the extract, the electromagnet is energized and the magnetic beads are attracted to the side of the liquid conducting tip 51. Therefore, the magnetic beads or other magazines are prevented from being transferred together in the process of sufficiently and efficiently transferring the extraction materials (protein and nucleic acid) from the extraction bin to the reaction bin.
As a preferable aspect of the present invention, in the embodiment of the present invention, two reaction liquid storage chambers 70 are provided. Of course, in other embodiments of the present invention, the reaction liquid storage chambers 70 may be provided in one or other numbers, so as to implement the corresponding biochemical reactions in the reaction chamber of the present invention.
In addition, in the embodiment of the present invention, the reaction liquid storage chamber 70 includes a piston type push-pull structure 71, and the piston type push-pull structure 71 is provided with a puncture structure 73; a cavity for containing the reaction liquid is formed in the reaction liquid storage cavity 70, and the bottom of the cavity is provided with a packaging film 72; when the reaction liquid needs to be injected into the reaction chamber 20, the piston type push-pull structure 71 is pushed, the puncture structure 73 pierces the packaging film 72 at the bottom of the cavity, and the reaction liquid passes through the reaction liquid injection channel 33 and is injected into the reaction chamber 20 through the reaction liquid conduit.
In the embodiment of the present invention, the extraction chamber 10 and the reaction chamber 20 are both detachable structures for easy installation and replacement. Wherein, the extraction bin 10 and the reaction bin 20 are fixed on the main structure of the biological reaction device provided with the microfluidic structure by a thread 23. Of course, in other embodiments of the present invention, a snap or other common detachable fixing means can be selected according to specific needs to fix the extraction chamber 10 and the reaction chamber 20 to the main structure of the bioreactor.
In the embodiment of the present invention, in order to scientifically arrange the microfluidic structure 30 and other corresponding structures so as to achieve good liquid flow conduction and connection, the microfluidic structure 30 is provided with a positioning region and a conduction micro-hole of a structure related thereto. The mixing driver 40 is fixed to the microfluidic structure 30, the mixing driver 40 is provided with a mixing nozzle 41 penetrating through the microfluidic structure 30 and inserted below the liquid level in the extraction chamber 10, and a positioning through hole 410 corresponds to the mixing driver 40. The pneumatic pump 50 is installed above the micro-fluidic structure 30, and is connected with the gas guide channel 31 through the first micropore 500, and the second micropore 520 is further arranged below the micro-fluidic structure 30 and is communicated with the extraction bin 10, so that pressure gas generated in the pneumatic pump 50 is transmitted into the extraction bin 10. After the pressurized gas acts on the extracting solution in the extracting chamber 10, the extracting solution is made to flow out to the extracting solution channel 32 through the liquid conducting suction head 51, and the micro-pore three 510 connecting the liquid conducting suction head 51 and the extracting solution channel 32 is arranged below the micro-fluidic structure 30. The extracting solution in the extracting chamber 10 passes through the liquid conducting suction head 51 and enters the reaction chamber 20 through the extracting solution channel 32 and the liquid conduit 21, and the micro-hole four 530 arranged below the micro-fluidic structure 30 is connected with the extracting solution channel 32 and the liquid conduit 21. Before the reaction chamber 20 reacts, the reaction solution in the reaction solution storage chamber 70 passes through the reaction solution injection channel 33 and is injected into the reaction chamber 20 through the reaction solution conduit 22, and the reaction solution injection channel 33 is connected and is a micropore 700 through the reaction solution conduit 22.
The biological reaction device provided with the microfluidic structure disclosed by the embodiment is communicated with the extraction bin and the reaction bin by the microfluidic structure, and the extracted materials (protein and nucleic acid) are fully and efficiently transferred from the extraction bin to the reaction bin by the microfluidic structure, so that the experimental process is simplified, the experimental efficiency is improved, and the possibility of pollution during sample transfer is reduced. Specifically, the biological reaction device with the microfluidic structure is provided with the mixing driver, and materials in the extraction bin are mixed and disturbed by pushing and pulling the mixing driver, so that the extracts adsorbed on the magnetic beads are sufficiently eluted; in addition, through being provided with the pneumatic pump, after being exerted pressure to this pneumatic pump, the pressure gas in the pneumatic pump gets into and draws the storehouse through the gas guide passageway, under the pressure gas effect, draws the extract in the storehouse and passes through liquid conduction suction head and get into in the reaction storehouse via extract passageway and liquid conduit to realize fully, high-efficiently transferring the extraction material (protein, nucleic acid) from drawing the storehouse to the reaction storehouse, simplify the experimental procedure and improve experimental efficiency and reduce the contaminated possibility when the sample shifts.
In addition, from the overall structure, the biological reaction device provided with the microfluidic structure has a simple structure and is convenient to operate, the structures of the microfluidic structure, the pressing type valve, the detachable extraction bin and reaction bin, the piston type reaction liquid storage cavity, the mixing driver, the pneumatic pump and the like which are arranged on the biological reaction device can be well matched with an external automatic device, particularly a pressing or pushing device with an air cylinder or mechanically pushed, and related external equipment which realizes simple actions such as the like can be greatly simplified, so that the complexity of equipment matched with the biological reaction device is effectively reduced.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.
Claims (10)
1. A biological reaction device provided with a microfluidic structure is characterized by comprising an extraction bin, a reaction bin and the microfluidic structure for transferring a macromolecular extract in the extraction bin to the reaction bin;
the mixing driver is fixed on the micro-fluidic structure, a mixing spray head penetrating through the micro-fluidic structure and inserted below the liquid level in the extraction bin is arranged on the mixing driver, and materials in the extraction bin can be mixed by pushing and pulling the mixing driver;
a pneumatic pump is further arranged above the micro-fluidic structure, and a gas guide channel for communicating the extraction bin with the pneumatic pump and an extracting solution channel for communicating the extraction bin with the reaction bin are arranged in the micro-fluidic structure; one end of the extracting solution channel is communicated with a liquid conduction suction head arranged in the extracting bin, and the other end of the extracting solution channel is communicated with a liquid guide pipe arranged in the reaction bin;
after the pneumatic pump is pressed, pressure gas in the pneumatic pump enters the extraction bin through the gas guide channel, and under the action of the pressure gas, an extracting solution in the extraction bin passes through the liquid conduction suction head and enters the reaction bin through the extracting solution channel and the liquid guide pipe;
a reaction liquid storage cavity is further arranged above the micro-fluidic structure, a reaction liquid injection channel is further arranged in the micro-fluidic structure, and the reaction liquid injection channel is communicated with the reaction bin through a reaction liquid injection guide pipe arranged above the reaction bin; before the reaction chamber reacts, the reaction liquid in the reaction liquid storage cavity passes through the reaction liquid injection channel and is injected into the reaction chamber through the reaction liquid injection conduit, and the reaction liquid is mixed with the extracting solution which is obtained from the extracting chamber and transferred into the reaction chamber to react.
2. The bioreactor apparatus provided with a microfluidic structure according to claim 1, wherein: and a liquid valve capable of blocking or opening the extracting solution channel is also arranged above the microfluidic structure.
3. The bioreactor apparatus provided with a microfluidic structure according to claim 2, wherein: the valve is a press type valve, after a valve button on the valve is pressed, a valve channel is communicated with the extracting solution channel, and the extracting solution channel is opened; after the valve button on the valve is pulled up, the valve channel deviates from the extracting solution channel, and the extracting solution channel is closed.
4. The bioreactor apparatus provided with a microfluidic structure according to claim 1, wherein: the extraction bin contains magnetic beads to which extracts of macromolecules including nucleic acid or protein are adsorbed.
5. The bioreactor apparatus provided with a microfluidic structure according to claim 4, wherein: an electromagnet for adsorbing magnetic beads in the extraction bin is arranged outside the extraction bin, and the electromagnet is arranged on one side, far away from the liquid conduction suction head, outside the extraction bin; when the materials in the mixed extraction bin are pushed and pulled so as to effectively elute the extract adsorbed on the magnetic beads in the extraction bin, the electromagnet is in a non-electrified state, and the magnetic beads are dispersed in the extraction bin; when the extract needs to be transferred from the extraction bin to the reaction bin for effective elution, the electromagnet is in a powered state, and the magnetic beads are adsorbed on the side away from the liquid conduction suction head.
6. The bioreactor apparatus provided with a microfluidic structure according to claim 1, wherein: the reaction liquid storage cavity is provided with one or more than two reaction liquid storage cavities.
7. The bioreactor apparatus provided with a microfluidic structure according to claim 1, wherein: the reaction liquid storage cavities are two in number.
8. The bioreactor apparatus provided with a microfluidic structure according to claim 1, wherein: the reaction liquid storage cavity comprises a piston type push-pull structure, and the piston type push-pull structure is provided with a puncture structure; a cavity for containing the reaction liquid is formed in the reaction liquid storage cavity, and the bottom of the cavity is provided with a packaging film; when the reaction liquid needs to be injected into the reaction bin, the piston type push-pull structure is pushed, the puncture structure pierces the packaging film at the bottom of the cavity, and the reaction liquid passes through the reaction liquid injection channel and is injected into the reaction bin through the reaction liquid guide pipe.
9. The bioreactor apparatus provided with a microfluidic structure according to claim 1, wherein: the extraction bin and the reaction bin are both detachable structures.
10. The biological reaction device provided with the microfluidic structure according to claim 9, wherein: the extraction bin and the reaction bin are fixed on a main body structure of the biological reaction device provided with the microfluidic structure in a buckling or threaded mode.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1534296A (en) * | 2003-03-31 | 2004-10-06 | ������������ʽ���� | Biochemical reaction box |
| CN1993459A (en) * | 2004-08-03 | 2007-07-04 | 新加坡科技研究局 | Method and device for the treatment of biological samples |
| WO2011074456A1 (en) * | 2009-12-16 | 2011-06-23 | 株式会社日立ハイテクノロジーズ | Sample processing device, sample processing method, and reaction container used in these device and method |
| CN104673625A (en) * | 2015-02-13 | 2015-06-03 | 西安交通大学 | Automatic reaction device and method for pretreating cells |
| CN105733935A (en) * | 2014-12-12 | 2016-07-06 | 余家昌 | Preparation and detection reagent card case for nucleic acid or protein |
| CN107723210A (en) * | 2017-11-19 | 2018-02-23 | 杭州安弼晟生物科技有限公司 | Novel nucleic acids detect micro flow control chip device |
| CN107893020A (en) * | 2017-11-27 | 2018-04-10 | 深圳华炎微测医疗科技有限公司 | Molecule diagnoses micro-fluidic chip and molecule diagnosis micro-fluidic chip system and their application |
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| CN1534296A (en) * | 2003-03-31 | 2004-10-06 | ������������ʽ���� | Biochemical reaction box |
| CN1993459A (en) * | 2004-08-03 | 2007-07-04 | 新加坡科技研究局 | Method and device for the treatment of biological samples |
| WO2011074456A1 (en) * | 2009-12-16 | 2011-06-23 | 株式会社日立ハイテクノロジーズ | Sample processing device, sample processing method, and reaction container used in these device and method |
| CN105733935A (en) * | 2014-12-12 | 2016-07-06 | 余家昌 | Preparation and detection reagent card case for nucleic acid or protein |
| CN104673625A (en) * | 2015-02-13 | 2015-06-03 | 西安交通大学 | Automatic reaction device and method for pretreating cells |
| CN107723210A (en) * | 2017-11-19 | 2018-02-23 | 杭州安弼晟生物科技有限公司 | Novel nucleic acids detect micro flow control chip device |
| CN107893020A (en) * | 2017-11-27 | 2018-04-10 | 深圳华炎微测医疗科技有限公司 | Molecule diagnoses micro-fluidic chip and molecule diagnosis micro-fluidic chip system and their application |
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