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CN109221190A - A kind of compounded pesticides effectively preventing cotten aphid - Google Patents

A kind of compounded pesticides effectively preventing cotten aphid Download PDF

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Publication number
CN109221190A
CN109221190A CN201811181517.XA CN201811181517A CN109221190A CN 109221190 A CN109221190 A CN 109221190A CN 201811181517 A CN201811181517 A CN 201811181517A CN 109221190 A CN109221190 A CN 109221190A
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CN
China
Prior art keywords
cotten aphid
imidacloprid
compounded pesticides
double
aphid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811181517.XA
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Chinese (zh)
Inventor
高希武
马康生
汤秋玲
刘晓岚
解晓平
宋敦伦
梁沛
史雪岩
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China Agricultural University
Original Assignee
China Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by China Agricultural University filed Critical China Agricultural University
Priority to CN201811181517.XA priority Critical patent/CN109221190A/en
Publication of CN109221190A publication Critical patent/CN109221190A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N51/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a kind of compounded pesticides for effectively preventing cotten aphid.For the technical problem for solving Insecticide Resistance of Cotton Aphids, the present invention is on the basis of a large amount of indoor virulences screen, it was found that double third ring worm esters compound the generation that can effectively delay Insecticide Resistance of Cotton Aphids with imidacloprid, and there is synergistic function to the prevention and treatment of cotten aphid, it provides for the integrated pest management of cotten aphid and new effectively prevents means.Present invention reduces Pesticide use amounts, to reduce costs and reduce the pollution to environment, are conducive to the resistance management of cotten aphid, popularization and application have huge economic benefit and social benefit.

Description

A kind of compounded pesticides effectively preventing cotten aphid
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of compounded pesticides for effectively preventing cotten aphid.
Background technique
Cotten aphid (cotton aphids, Aphis gossypii Glover) is a kind of universal important pests, It can the feeding in 700 various plants including cotton, cucumber etc..Cotten aphid mainly passes through direct feeding plant juice and propagation Plant virus causes damages, and causes huge economic loss to agricultural production.Cotten aphid is fast with reproduction speed, population quantity is big, The features such as serious extent, for a long time, chemical prevention are always the main means of China's cotton aphid control.Due to chemical pesticide Long-term a large amount of uses, cotten aphid produce height to a variety of insecticides such as organic phosphorus, carbamate, pyrethroid, anabasine Horizontal resistance.
Insecticide, which is used for a long time, so that cotten aphid is developed drug resistance, and the simple usage amount for increasing pesticide more pollutes the environment.But Recently as a large amount of frequent uses of anabasine insecticide, cotten aphid also gets worse the resistance of such medicament.Cui Li etc. (2016) result of study shows that Aksu Prefecture cotten aphid has reached 26.3 times to the resistance of imidacloprid, has reached medium resistance water It is flat.And the Aphis gossypiipopulation in Koo etc. (2014) report some areas of South Korea is to Acetamiprid, clothianidin, imidacloprid, thiacloprid and thiophene worm The neonicotinoid insecticides such as piperazine produce the result of study of high horizontal resistance, and wherein cotten aphid is to imidacloprid, Acetamiprid and clothianidin Resistant multiple respectively reached 1500,2600 and 14000 times.
Double third ring worm esters (Afidopyropen) be botanical pesticide, structure novel and have completely new mechanism of action, It can be used for preventing and treating rice, fruit tree, vegetables, mini pest such as scale insect, thrips, leafhopper, powder on imtertilled crop and ornamental plant Lice and wood louse etc., either foliar treatment, seed treatment or soil treatment have good result, and toxicity is very low.
Imidacloprid (Imidacloprid), be it is a kind of efficiently, the strong anabasine insecticide of wide spectrum, absorbability, have low Poison, low-residual, lasting period are long, the features such as to people, animal and natural enemies security, and have and tag, the multiple actions such as stomach toxicity and interior suction.Mainly For preventing and treating agriculturally important sucking pest, such as aphid, plant hopper, thrips.
Summary of the invention
For the technical problem for solving Insecticide Resistance of Cotton Aphids, the present invention is on the basis of a large amount of indoor virulences screen, discovery double third Ring worm ester compounds the generation that can effectively delay Insecticide Resistance of Cotton Aphids with imidacloprid, and has synergistic make to the prevention and treatment of cotten aphid With being provided for cotten aphid evil comprehensive treatment and new effectively prevent means.
The present invention adopts the following technical scheme that.
A kind of compounded pesticides effectively preventing cotten aphid, active constituent include double third ring worm esters and imidacloprid, the two matter Amount is than being 1:(5-10).
Further, the mass ratio of double third ring worm esters and imidacloprid is 1:5 or 1:10 in the compounded pesticides.
Further, the active constituent of the compounded pesticides is made of double third ring worm esters with imidacloprid.
The research of the invention finds that double third ring worm esters compound within the scope of aforementioned proportion with imidacloprid can effectively delay cotten aphid The generation of drug resistance also has synergistic function to the prevention and treatment of cotten aphid, achieves unexpected technical effect.Wherein, double In optimum proportioning 1:5, co-toxicity coefficient reaches 159.15 for third ring worm ester and imidacloprid, has apparent synergistic effect, improves Control efficiency.
Further, the available carrier of pesticide field or auxiliary material are also contained in the compounded pesticides.
Further, the compounded pesticides can be made into the available various dosage forms of pesticide field.
Aforementioned compounded pesticides can be prepared by conventional method in that art.
The present invention also provides aforementioned compounded pesticides in prevention and control of aphides disease or in the pesticide for preparing prevention and control of aphides disease In application.
On the basis of common knowledge of the art, above-mentioned each optimum condition can be combined with each other each preferably to get the present invention Example.
Cotten aphid of the present invention is Aphis gossypii Glover, is a kind of insect of Aphidiadae Aphis, is commonly called as aphid.
The present invention is especially the study found that double third ring worm esters are compounded with imidacloprid, its mechanism of action in terms of prevention and control of aphides It is unique that cross resistance is especially not present, delay the generation and development of Insecticide Resistance of Cotton Aphids, reduced Pesticide use amount, to drop Low cost and reduce the pollution to environment, is conducive to the resistance management of cotten aphid, popularization and application have huge economic benefit And social benefit.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..It is not specified in embodiment specific Technology or conditions person, described technology or conditions according to the literature in the art, or carried out according to product description.It is used Production firm person is not specified in reagent or instrument, is the conventional products that can be commercially available by regular distributor.
Experimental example 1
This experiment using double third ring worm esters, imidacloprid as reagent agent, using blade medicine embrane method carry out toxicity test, single dose with Each ratio mixture carries out blade medicine embrane method toxicity test, calculates LC50, co-toxicity coefficient (CTC) and optimum proportioning.
1. research object
The cotton Aphid in laboratory rearing mostly generation.Cotton Aphid picks up from cotton in Xinjiang field, and non-turn of indoor culture Gene cotton seedling raising mostly generation.
2. method
Reagent agent double third ring worm esters, imidacloprid carry out indoor toxicity test, including double third rings using blade medicine embrane method Worm ester, Imidacloprid single dose toxicity test;The toxicity test of double third ring worm esters and each ratio mixture of imidacloprid.To toxicity test result It is calculated, calculates LC50, co-toxicity coefficient (CTC), obtain optimum proportioning.
The double third ring worm esters of reagent agent, imidacloprid are raw medicine, with acetone solution, 4 DEG C of preservations.The medicament of toxicity test is dense Degree is set as 6~7, and sets three simple repetitions;Each ratio mixture is mixing before dilution.
Blade used is non-transgenic cotton (plantation in constant incubator).
The preparation and dilution of 2.1 raw medicine mother liquors
Double third ring worm esters are that 10:1,5:1,1:1,1:5,1:10 are compounded according to mass ratio with imidacloprid, and raw medicine is molten Solution is deposited in flask for medicinal preparations after acetone, volumetric flask constant volume, and sealed membrane sealing, 4 DEG C of refrigerators save.Before toxicity test, with containing The distilled water of 0.05% (v/v) Triton X-100 is diluted to each concentration, spare.
The 2.2 raw selections and use for surveying blade
The leaf dish that the non-Bt cotton leaf of fresh cleaning is broken into diameter 2cm with punch, soaks in the medical fluid diluted It takes out, dries after stain 15s.Then, the leaf dish back side is placed in the 12 hole cell culture that bottom is covered with 2% agar gel of 2mL upward In plate.It is control with the distilled water containing 0.05% (v/v) Triton X-100.
The selection of 2.3 toxicity test examination aphids
The cotton Aphid of indoor raising is selected, the consistent aptery adult aphid of picking health is tested, each prepared Leaf dish place 30 aphids, sealed with rice paper, be placed in growth cabinet raising (23 ± 1 DEG C of temperature, relative humidity 50% ~75%, illumination 16L:8D).
2.4 result inspections and death standard judgement
Handle inspection result after 48h, mesopodium is motionless, and with small writing brush touch it is completely motionless be denoted as death, with control The death rate is effectively measurement less than 10%, and is corrected with the control death rate.6~7 concentration, Mei Genong is arranged in each medicament 3 repetitions are spent, data processing analyzes software using POLO Plus, calculates LC50, calculate the LC of single dose and each proportion mixture50And 95% confidence limit, virulence regression equation etc..And according to calculated result, mixture co-toxicity coefficient (CTC) is found out using Sun Yunpei's Method, altogether Malicious coefficient (CTC value) >=120 shows as synergistic effect;Co-toxicity coefficient (CTC value)≤80 is antagonism;80 < co-toxicity coefficient (CTC value) < 120 shows as summation action, compares synergy situation according to CTC value, obtains the best of double third ring worm esters and imidacloprid Proportion.
Test result is shown in Table 1.
1 pair of the third ring worm ester of table compounds The Toxicity Determination result with imidacloprid
It is malicious altogether when double third ring worm esters are compounded with imidacloprid than being respectively 1:5 and 1:10 it can be seen from 1 test result of table Coefficient is greater than 120, shows as synergistic function.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.

Claims (7)

1. a kind of compounded pesticides for effectively preventing cotten aphid, active constituent includes double third ring worm esters and imidacloprid, the two quality Than for 1:(5-10).
2. compounded pesticides according to claim 1, which is characterized in that the matter of the double third ring worm esters and imidacloprid of active constituent Amount is than being 1:5 or 1:10.
3. compounded pesticides according to claim 1 or 2, which is characterized in that the active constituent by double third ring worm esters with Imidacloprid composition.
4. compounded pesticides according to claim 1-3, which is characterized in that also contain the available load of pesticide field Body or auxiliary material.
5. compounded pesticides according to claim 1-4, which is characterized in that the cotten aphid is Aphis gossypii Glover。
6. any one of the claim 1-5 compounded pesticides are in prevention and control of aphides disease or in the pesticide for preparing prevention and control of aphides disease In application.
7. application according to claim 6, which is characterized in that the cotten aphid is Aphis gossypii Glover.
CN201811181517.XA 2018-10-11 2018-10-11 A kind of compounded pesticides effectively preventing cotten aphid Pending CN109221190A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110200004A (en) * 2019-07-04 2019-09-06 山东省农业科学院植物保护研究所 A kind of compound drug composition for preventing and treating Bemisia tabaci, melon aphid
CN111374144A (en) * 2018-12-27 2020-07-07 燕化永乐(乐亭)生物科技有限公司 Insecticidal composition
CN115281201A (en) * 2022-07-15 2022-11-04 新疆天禾嘉信农业科技有限公司 Insecticide compounded by diclocycline dispersant and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101626687A (en) * 2007-03-08 2010-01-13 明治制果株式会社 Pest control composition
CN105432653A (en) * 2015-12-18 2016-03-30 广东中迅农科股份有限公司 Insecticidal composition

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101626687A (en) * 2007-03-08 2010-01-13 明治制果株式会社 Pest control composition
CN105432653A (en) * 2015-12-18 2016-03-30 广东中迅农科股份有限公司 Insecticidal composition

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
陈敏 等: "新型杀虫剂双丙环虫酯对黄瓜蚜虫的田间防效", 《农药》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111374144A (en) * 2018-12-27 2020-07-07 燕化永乐(乐亭)生物科技有限公司 Insecticidal composition
CN110200004A (en) * 2019-07-04 2019-09-06 山东省农业科学院植物保护研究所 A kind of compound drug composition for preventing and treating Bemisia tabaci, melon aphid
CN110200004B (en) * 2019-07-04 2021-07-06 山东省农业科学院植物保护研究所 Compound medicament composition for preventing and treating bemisia tabaci and aphis cucumeriae
CN115281201A (en) * 2022-07-15 2022-11-04 新疆天禾嘉信农业科技有限公司 Insecticide compounded by diclocycline dispersant and application thereof

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Application publication date: 20190118

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