CN109187943B - Anti-interference reagent cup and preparation method of anti-interference coating in reagent cup - Google Patents
Anti-interference reagent cup and preparation method of anti-interference coating in reagent cup Download PDFInfo
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- CN109187943B CN109187943B CN201810973454.5A CN201810973454A CN109187943B CN 109187943 B CN109187943 B CN 109187943B CN 201810973454 A CN201810973454 A CN 201810973454A CN 109187943 B CN109187943 B CN 109187943B
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The invention discloses an anti-interference reagent cup and a preparation method of an anti-interference coating in the reagent cup, and solves the problems that the existing sealing agent can participate in immune reaction in the whole process, and the immune reaction of antigen and antibody is reduced while interference is inhibited. The preparation method of the anti-interference coating comprises the steps of diluting a sealing agent to 0.5-1.5 mg/mL by using CB, adding the sealing agent into a cup tube of a reagent cup, standing for 20-24 hours at the temperature of 2-8 ℃, cleaning the cup tube by using purified water for more than two times, and spin-drying; and (4) drying the cup tube at the temperature of below 40 ℃. The invention can make the interference substance in the sample captured by the anti-interference coating, therefore, the sample without interference enters the following immune reaction, and the immune reaction capability can be effectively improved.
Description
Technical Field
The invention relates to the field of fluorescence immunochromatography, in particular to an anti-interference reagent cup and a preparation method of an anti-interference coating in the reagent cup.
Background
Immunodiagnosis is the use of immunological theories, techniques and methods to diagnose a variety of diseases and determine immune status. The immunodiagnostic reagent has the most varieties in a diagnostic kit, is widely applied to hospitals, blood stations and physical examination centers, and is mainly used for hepatitis detection, venereal disease detection, tumor detection, pregnancy detection and the like.
The tracer is classified into radioimmunoassay, enzyme-linked immunosorbent assay, fluorescence assay, chemiluminescence assay, colloidal gold assay, latex assay, etc.
The fluorescence immunochromatography is a membrane detection technology based on antigen-antibody specific immunoreaction. The technology takes strip-shaped fiber chromatography materials fixed with a detection line (coated antibody or coated antigen) and a quality control line (anti-antibody) as a stationary phase, a test solution as a mobile phase, a fluorescence labeled antibody or antigen fixed on a connecting pad, and an analyte to be analyzed moves on the chromatography strip through capillary action. For macromolecular antigens (proteins, viruses, pathogenic bacteria and the like) with a plurality of antigenic determinants, a sandwich type double-antibody sandwich immunochromatography method is generally adopted, namely, an object to be detected is firstly combined with a fluorescence labeling antibody under the action of a mobile phase, and then is combined with a coating antibody to form a sandwich type double-antibody sandwich when reaching a detection line. The instrument reads the fluorescence signal of the detected area and calculates the concentration of the target substance.
It is well known that immunoassays, particularly in the sandwich format, are susceptible to interference by many endogenous antibodies, including anti-animal species antibodies, rheumatoid factor and other autoantibodies or xenotropic antibodies. The interfering antibodies may be of any type (IgG, IgA, IgM or IgE) capable of binding to antibodies of other species, or other cellular components and reagent components. These endogenous antibodies cause false positives or false negatives by altering the binding capacity of the target test substance or detection reagent antibody, giving detection results that do not match the patient's true clinical condition.
Whereas in the prior art, commercial blocking agents are typically used to inhibit two major classes of interference:
1. rheumatoid Factor (RF), a typical IgM class antibody, recognizes the Fc fragment of IgG. RF binds not only human IgG but also immunoglobulins of other species. RF is common in rheumatoid patients, autoimmune disease patients, or other healthy people. An effective way to control RF interference is to incorporate anti-RF-related antibodies in a closed system.
2. Heterophilic antibodies, which refer to human antibodies capable of binding to animal antibodies, antigens or autoantigens, are more accurately described as those polyreactive antibodies that react with antigens of unknown or unknown definition. The presence of such antibodies is found in 40% of the population. These antibodies have low affinity and weak reactivity, but can bridge the capture antibody and the binding antibody in the absence of the test substance, and effectively compete with the test substance for the corresponding binding site. Interference can be minimized by the addition of normal serum or purified antibodies of the same species. These antibodies saturate the binding site of the heterophilic antibody, thereby blocking the production of interference.
Because the traditional blocking agent using method is to directly add the blocking agent into a reaction system, the blocking agent can participate in immune reaction in the whole process, and the immune reaction of antigen and antibody is reduced while interference is inhibited.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the existing sealing agent can participate in immune reaction in the whole process, the problem of immune reaction of antigen and antibody is solved while interference is inhibited, and the anti-interference reagent cup and the preparation method of the anti-interference coating in the reagent cup are provided.
The invention is realized by the following technical scheme:
a preparation method of an anti-interference coating in a reagent cup comprises the following steps:
diluting the sealing agent to 0.5-1.5 mg/mL by using CB, adding the sealing agent into a cup tube of a reagent cup, standing for 20-24 hours at the temperature of 2-8 ℃, cleaning the cup tube by using purified water for more than two times, and spin-drying; and (4) drying the cup tube at the temperature of below 40 ℃.
According to the invention, through the arrangement of the anti-interference coating, in the process, the interference substances in the sample are captured by the coating, and the captured interference substances and the sealant are fixed in the cup tube and cannot enter subsequent immunoreaction and detection along with the sample liquid, so that the immunoreaction capability is greatly improved, and the effect is very obvious.
Further, the CB is an alkaline buffer solution, and the baking time in the oven is 20-24 hours.
Further, the CB comprises NaHCO3 7.56g/L，Na2CO31.06g/L, NaCl 7.36 g/L; the pH of the CB was 9.0.
Further, the blocking agent is Super ChemiBlock Heterophile from Merck-millipore.
Further, the cup tube was dried in an oven at 37 ℃.
An anti-interference reagent cup comprises a base frame, and a suction head loading hole, a sample cavity, a first reagent tube, a second reagent tube and a reagent clamping strip which are arranged on the base frame from left to right in sequence; a sample adding window and a detection window are correspondingly arranged above the reagent card strip; the anti-interference coatings prepared by the method are arranged on the first reagent tube and the second reagent tube.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. interfering substances in the sample are captured by the anti-interference coating, so that the sample without interference enters the subsequent immune reaction, and the immune reaction capability of the sample can be effectively improved;
2. compared with the traditional chromatography method, the coating improves the reaction by 20-30% under the condition of unchanged background.
Drawings
The accompanying drawings, which are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention. In the drawings:
FIG. 1 is a schematic structural diagram of an anti-interference reagent cup according to the present invention.
1-base frame, 2-suction head loading hole, 3-sample cavity, 4-first reagent tube, 5-second reagent tube, 6-sample adding window and 7-detection window.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.
Example 1
A preparation method of an anti-interference coating in a reagent cup comprises the following steps: diluting a sealing agent to 1mg/mL by using CB, adding the sealing agent into a reagent tube, standing for 20-24 hours at the temperature of 2-8 ℃, then cleaning the reagent tube by using purified water for more than two times, and spin-drying; and (3) placing the reagent tube in a 37 ℃ oven for drying, wherein the drying time in the 37 ℃ oven is usually 20-24 hours.
An anti-interference reagent cup comprises a base frame 1, and a sucker loading hole 2, a sample cavity 3, a first reagent tube 4, a second reagent tube 5 and a reagent clamping strip which are arranged on the base frame 1 in sequence from left to right; a sample adding window 6 and a detection window 7 are correspondingly arranged above the reagent card strip; the anti-interference coatings prepared by the preparation method of the anti-interference coating in the reagent cup are arranged on the first reagent tube 4 and the second reagent tube 5.
The CB described in this embodiment refers to a common alkaline buffer, and the pH of the buffer is 9.0, and the specific formulation is: NaHCO 23 7.56g/L，Na2CO31.06g/L, NaCl 7.36 g/L. The blocking agent used in this example was SuperChemiBlock Heterophile from Merck-millipore.
The specific use process of the anti-interference reagent cup is as follows:
adopt the suction head on suction head loading hole 2 to absorb the sample in sample cavity 3, after adding the sample into first reagent pipe 4 and reacting with first reagent, reuse suction head absorbs reaction liquid in first reagent pipe 4 and adds into second reagent pipe 5, after reaction liquid in first reagent pipe 4 and the liquid in second reagent pipe 5 react, absorb again and drop add to application of sample window 6, add to the reagent card strip of application of sample window 6 below, liquid moves to detection window 7 positions department on the reagent card strip, can detect out signal value in detection window 7 positions department through detecting instrument.
Example 2
The difference between this embodiment and embodiment 1 is that the concentration of the added blocking agent in this embodiment is different, and the following is set in this embodiment:
diluting a sealing agent to 0.5mg/mL by using CB, adding the sealing agent into a reagent tube, standing for 20-24 hours at the temperature of 2-8 ℃, cleaning the reagent tube by using purified water for more than two times, and spin-drying; and (3) placing the reagent tube in a 37 ℃ oven for drying.
Example 3
The difference between this embodiment and embodiment 1 is that the concentration of the added blocking agent in this embodiment is different, and the following is set in this embodiment:
diluting a sealing agent to 1.5mg/mL by using CB, adding the sealing agent into a reagent tube, standing for 20-24 hours at the temperature of 2-8 ℃, cleaning the reagent tube by using purified water for more than two times, and spin-drying; and (3) placing the reagent tube in a 37 ℃ oven for drying.
The anti-interference reagent cup prepared in the embodiment 1 and the traditional reagent cup are respectively detected in the same reaction system, and the operation process and the parameters of the detection are completely the same. The traditional reagent cup has no anti-interference coating, but the optimal dosage of the sealant is directly added into the reaction system during detection. The results of the measurements are shown in Table 1 below.
TABLE 1
As can be seen from table 1 above: compared with the traditional chromatography method, the coating improves the reaction by 20-30% under the condition of unchanged background.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (6)
1. A preparation method of an anti-interference coating in a reagent cup is characterized by comprising the following steps:
diluting the sealing agent to 0.5-1.5 mg/mL by using CB, adding the sealing agent into a cup tube of a reagent cup, standing for 20-24 hours at the temperature of 2-8 ℃, cleaning the cup tube by using purified water for more than two times, and spin-drying; and (3) drying the cup tube below 40 ℃, capturing the interference substances in the sample by the coating, fixing the captured interference substances and the sealant in the cup tube, and preventing the captured interference substances and the sealant from entering subsequent immunoreaction and detection along with the sample liquid.
2. The method for preparing the anti-interference coating in the reagent cup according to claim 1, wherein the CB is an alkaline buffer solution, and the baking time in the oven is 20-24 hours.
3. The method as claimed in claim 2, wherein the CB comprises NaHCO3 7.56g/L，Na2CO31.06g/L, NaCl 7.36 g/L; the pH of the CB was 9.0.
4. The method for preparing an anti-interference coating in a reagent cup as claimed in claim 1, wherein the sealant is Super ChemiBlock Heterophile of Merck-millipore company.
5. The method for preparing an anti-interference coating in a reagent cup according to claim 1, wherein the cup tube is dried in an oven at 37 ℃.
6. An anti-interference reagent cup comprises a base frame (1), and a sucker loading hole (2), a sample cavity (3), a first reagent tube (4), a second reagent tube (5) and a reagent clamping strip which are arranged on the base frame (1) from left to right in sequence; a sample adding window (6) and a detection window (7) are correspondingly arranged above the reagent card strip; characterized in that the first reagent tube (4) and the second reagent tube (5) are both provided with an anti-interference coating prepared by the method of any one of claims 1 to 5.
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Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5441895A (en) * | 1993-12-07 | 1995-08-15 | Jakubowicz; Raymond F. | Reagent cup shape allowing stacking without dislodging reagent |
| WO2005073733A1 (en) * | 2002-11-06 | 2005-08-11 | Eduardo Cadorniga Martinez | Self-performed abo blood group determination method |
| WO2009059784A1 (en) * | 2007-11-07 | 2009-05-14 | Leukocare Ag | Biocompatible three dimensional matrix for the immobilization of biological substances |
| CN101632023A (en) * | 2007-03-16 | 2010-01-20 | 雷迪奥米特医学公司 | Reagent cup device |
| EP2270507A1 (en) * | 2008-03-24 | 2011-01-05 | FUJIFILM Corporation | Method for immobilization, physiologically active substance-immobilized carrier, carrier for immobilization, carrier, and process for producing carrier |
| US8354274B2 (en) * | 2008-01-30 | 2013-01-15 | Geron Corporation | Synthetic surfaces for culturing cells in chemically defined media |
| CN103025758A (en) * | 2010-05-14 | 2013-04-03 | Abbvie公司 | IL-1 binding proteins |
| CN202916285U (en) * | 2012-11-12 | 2013-05-01 | 南京诺尔曼生物技术有限公司 | Whole blood immunochromatography device |
| CN103998932A (en) * | 2011-06-29 | 2014-08-20 | 中央研究院 | Capture, purification and release of biological substance using a surface coating |
| CN104502591A (en) * | 2014-11-10 | 2015-04-08 | 江苏宏泰格尔生物医学工程有限公司 | Pepsinogen I and II combined detection method and kit thereof |
| CN104515850A (en) * | 2013-09-30 | 2015-04-15 | 万冰 | Quick test device and quick test method |
| CN204330771U (en) * | 2015-01-07 | 2015-05-13 | 江阴康奈尔生物科技有限公司 | A kind of novel speed surveys epidermal stem cells kit |
| CN104761697A (en) * | 2014-01-02 | 2015-07-08 | 上海移宇科技有限公司 | Biosensor film and making method thereof |
| CN105073785A (en) * | 2012-10-22 | 2015-11-18 | 锡德克斯药物公司 | Alkylated cyclodextrin compositions and processes for preparing and using the same |
| CN105393119A (en) * | 2013-07-30 | 2016-03-09 | 生物辐射实验室股份有限公司 | Multiplex blocker beads for immunoassays |
| CN105758835A (en) * | 2016-05-04 | 2016-07-13 | 成都爱兴生物科技有限公司 | Homogeneous phase immunoassay POCT detection technique and system using same |
| CN105891468A (en) * | 2016-04-08 | 2016-08-24 | 四川新健康成生物股份有限公司 | Sample release pad treatment solution |
| CN105891306A (en) * | 2016-06-24 | 2016-08-24 | 北京化工大学 | Antibody modified nanoparticle electrophoresis flowing type ELISA (enzyme linked immunosorbent assay) method |
| CN205861692U (en) * | 2016-06-07 | 2017-01-04 | 艾托金生物医药（苏州）有限公司 | Cervical cancer high-risk HPV specific proteins mark immunocytochemical stain Quality Control flake products and test kit |
| CN206096145U (en) * | 2016-10-17 | 2017-04-12 | 赵军理 | A quick quantitative immune test paper strip for detecting liver is fine four |
| CN107290518A (en) * | 2017-07-31 | 2017-10-24 | 迈克生物股份有限公司 | A kind of reagent for eliminating immune response false positive |
| CN206832816U (en) * | 2017-03-17 | 2018-01-02 | 深圳市绿诗源生物技术有限公司 | Hog cholera antibody half-quantitative detection card |
| CN108226488A (en) * | 2017-11-30 | 2018-06-29 | 上海拜豪生物科技有限公司 | One heavy metal species fluorescence immunoassay detection method and its chromatography kit |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107794295B (en) * | 2017-11-09 | 2020-07-31 | 湖南工程学院 | Thrombin detection method and kit based on double Aptamer sandwich structure open loop-mediated isothermal amplification |
-
2018
- 2018-08-24 CN CN201810973454.5A patent/CN109187943B/en active Active
Patent Citations (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5441895A (en) * | 1993-12-07 | 1995-08-15 | Jakubowicz; Raymond F. | Reagent cup shape allowing stacking without dislodging reagent |
| WO2005073733A1 (en) * | 2002-11-06 | 2005-08-11 | Eduardo Cadorniga Martinez | Self-performed abo blood group determination method |
| CN101632023A (en) * | 2007-03-16 | 2010-01-20 | 雷迪奥米特医学公司 | Reagent cup device |
| WO2009059784A1 (en) * | 2007-11-07 | 2009-05-14 | Leukocare Ag | Biocompatible three dimensional matrix for the immobilization of biological substances |
| US8354274B2 (en) * | 2008-01-30 | 2013-01-15 | Geron Corporation | Synthetic surfaces for culturing cells in chemically defined media |
| EP2270507A1 (en) * | 2008-03-24 | 2011-01-05 | FUJIFILM Corporation | Method for immobilization, physiologically active substance-immobilized carrier, carrier for immobilization, carrier, and process for producing carrier |
| CN103025758A (en) * | 2010-05-14 | 2013-04-03 | Abbvie公司 | IL-1 binding proteins |
| CN103998932A (en) * | 2011-06-29 | 2014-08-20 | 中央研究院 | Capture, purification and release of biological substance using a surface coating |
| CN107315086A (en) * | 2011-06-29 | 2017-11-03 | 中央研究院 | Using face coat is to the capture of biological substance, purifying and discharges |
| CN105073785A (en) * | 2012-10-22 | 2015-11-18 | 锡德克斯药物公司 | Alkylated cyclodextrin compositions and processes for preparing and using the same |
| CN202916285U (en) * | 2012-11-12 | 2013-05-01 | 南京诺尔曼生物技术有限公司 | Whole blood immunochromatography device |
| CN105393119A (en) * | 2013-07-30 | 2016-03-09 | 生物辐射实验室股份有限公司 | Multiplex blocker beads for immunoassays |
| CN104515850A (en) * | 2013-09-30 | 2015-04-15 | 万冰 | Quick test device and quick test method |
| CN104761697A (en) * | 2014-01-02 | 2015-07-08 | 上海移宇科技有限公司 | Biosensor film and making method thereof |
| CN104502591A (en) * | 2014-11-10 | 2015-04-08 | 江苏宏泰格尔生物医学工程有限公司 | Pepsinogen I and II combined detection method and kit thereof |
| CN204330771U (en) * | 2015-01-07 | 2015-05-13 | 江阴康奈尔生物科技有限公司 | A kind of novel speed surveys epidermal stem cells kit |
| CN105891468A (en) * | 2016-04-08 | 2016-08-24 | 四川新健康成生物股份有限公司 | Sample release pad treatment solution |
| CN105758835A (en) * | 2016-05-04 | 2016-07-13 | 成都爱兴生物科技有限公司 | Homogeneous phase immunoassay POCT detection technique and system using same |
| CN205861692U (en) * | 2016-06-07 | 2017-01-04 | 艾托金生物医药（苏州）有限公司 | Cervical cancer high-risk HPV specific proteins mark immunocytochemical stain Quality Control flake products and test kit |
| CN105891306A (en) * | 2016-06-24 | 2016-08-24 | 北京化工大学 | Antibody modified nanoparticle electrophoresis flowing type ELISA (enzyme linked immunosorbent assay) method |
| CN206096145U (en) * | 2016-10-17 | 2017-04-12 | 赵军理 | A quick quantitative immune test paper strip for detecting liver is fine four |
| CN206832816U (en) * | 2017-03-17 | 2018-01-02 | 深圳市绿诗源生物技术有限公司 | Hog cholera antibody half-quantitative detection card |
| CN107290518A (en) * | 2017-07-31 | 2017-10-24 | 迈克生物股份有限公司 | A kind of reagent for eliminating immune response false positive |
| CN108226488A (en) * | 2017-11-30 | 2018-06-29 | 上海拜豪生物科技有限公司 | One heavy metal species fluorescence immunoassay detection method and its chromatography kit |
Non-Patent Citations (2)
| Title |
|---|
| "An ultra-sensitive monoclonal antibody-based fluorescent microsphere immunochromatographic test strip assay for detecting aflatoxin M1 in milk";Xiya Zhang et al;《Food Control》;20150830;第60卷;全文 * |
| "生物素化高分子涂层的制备与评价";卢天成 等;《高等学校化学学报》;20080430(第4期);全文 * |
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