CN109010124B

CN109010124B - Marine refined gynecological antibacterial gel and preparation method thereof

Info

Publication number: CN109010124B
Application number: CN201811129724.0A
Authority: CN
Inventors: 苏晓明; 迟乃秋; 邹圣灿; 贾凯
Original assignee: Qingdao Chenland Marine Biotechnology Co ltd
Current assignee: Qingdao Chenland Marine Biotechnology Co ltd
Priority date: 2018-09-27
Filing date: 2018-09-27
Publication date: 2021-11-23
Anticipated expiration: 2038-09-27
Also published as: CN109010124A

Abstract

The invention discloses a marine refined gynecological bacteriostatic gel and a preparation method thereof, and belongs to the technical field of private care products. The sea refined gynecological bacteriostatic gel is prepared from the following components in percentage by weight: 0.1-1.5% of chitosan quaternary ammonium salt, 0.05-1.5% of bacteriostatic agent, 0.6-6.0% of gel matrix, 0.5-4.5% of humectant, 0.5-3.0% of cell repair protective agent, 7.0-10.0% of plant extract, 3-5% of glycerol, 2-6% of 1, 3-butanediol, 0.005-0.1% of pH regulator and the balance of purified water. The ocean essence gynecological bacteriostatic gel is safe and mild, has no stimulation, has a good inhibiting effect on gynecological pathogenic bacteria, also has a good repairing effect on epithelial cells, and has a good treatment effect on gynecological diseases.

Description

Marine refined gynecological antibacterial gel and preparation method thereof

Technical Field

The invention belongs to the technical field of privacy care products, and particularly relates to a marine refined gynecological bacteriostatic gel and a preparation method thereof.

Background

Due to the specific physiological structure and constitution of women, and various factors such as increased competitive pressure, accelerated pace of work and life, and the like, the incidence of gynecological diseases is generally high in recent years, and the health of women is threatened continuously, wherein vaginitis, cervical erosion, breast cancer and the like are the most common gynecological diseases, and the gynecological diseases become the first killers of women. The investigation result shows that the total disease rate of the common diseases of the national women in 2010 in China is 28.8%, and among the common diseases of various women, vaginitis accounts for the first place and the disease rate is 13.2%.

Aiming at common gynecological problems such as pruritus vulvae, vaginitis, cervicitis and the like, daily sanitary nursing, prevention and maintenance and the like need to be enhanced. The ideal gynecological external product has good antibacterial and anti-inflammatory properties, safety, biocompatibility and the like, and simultaneously has the advantages of good stability, easy storage and convenient use, and compared with lotion, suppository and effervescent tablets, the gel can meet the requirements.

The invention patent with the publication number of CN104147108B discloses a bacteriostatic gel containing brown algae oligosaccharide, which mainly comprises the following active ingredients in parts by weight: 6 to 10 parts of brown alga oligosaccharide, 0.3 to 0.7 part of rough and tender, 0.1 to 0.3 part of common cnidium fruit and 0.3 to 0.7 part of lightyellow sophora root by weight are a new generation of biological preparation products for preventing vaginitis and cervicitis in gynecology, and the product creates the anti-infection pioneer of combining the traditional Chinese medicine and the brown alga oligosaccharide. However, the bacteriostatic gel has a part of the smell of traditional Chinese medicines in the using process, and the using feeling is not comfortable and fresh enough.

The invention patent with the publication number of CN101623251B provides a bionic propellant of female antibacterial gel, which is added with chlorhexidine acetate, triclosan and Germall-II antibacterial components in the formula, wherein triclosan and the like are pre-dissolved and then added into the system, and the process is relatively complex; in addition, the gel is added with ethanol, so that the gel is not suitable for ethanol allergic patients and has limitation in use.

Disclosure of Invention

Aiming at the problems in the prior art, the invention aims to provide the ocean refined gynecological bacteriostatic gel which can effectively prevent and treat gynecological inflammation and has good use experience and the preparation method thereof.

In order to achieve the purpose, the technical scheme of the invention is as follows:

the ocean refined gynecological bacteriostatic gel is prepared from the following components in percentage by weight:

0.1-1.5% of chitosan quaternary ammonium salt, 0.05-1.5% of bacteriostatic agent, 0.6-6.0% of gel matrix, 0.5-4.5% of humectant, 0.5-3.0% of cell repair protective agent, 7.0-10.0% of plant extract, 3-5% of glycerol, 2-6% of 1, 3-butanediol, 0.005-0.1% of pH regulator and the balance of purified water.

On the basis of the above-mentioned scheme,

the substitution degree of the chitosan quaternary ammonium salt is more than or equal to 90%, and the pH value (1.0%, 25 ℃) is 6.0-8.0;

the bacteriostatic agent is one of polyhexamethylene biguanide hydrochloride and chlorhexidine acetate;

the gel matrix is one or a composition of two of hydroxyethyl cellulose and hydroxypropyl methylcellulose;

the humectant is one or a composition of trehalose and an amino acid humectant;

the cell repair protective agent is one or a composition of two of MG-60 and fish collagen peptide;

the plant extract is a composition of witch hazel distillate and aloe vera extract;

the pH value regulator is one of lactic acid and acetic acid.

On the basis of the scheme, the plant extracting solution is composed of witch hazel distillate and aloe vera extracting solution according to a weight ratio of 4-5: 3-5.

On the basis of the scheme, the ocean refined gynecological bacteriostatic gel is prepared from the following components in percentage by weight:

1.0% of chitosan quaternary ammonium salt, 0.26% of polyhexamethylene biguanide hydrochloride, 1.0% of hydroxyethyl cellulose, 1.0% of trehalose, 1.0% of amino acid humectant, MG-600.6%, 1.0% of fish collagen peptide, 5.0% of witch hazel distillate, 5.0% of aloe barbadensis extract, 3.0% of glycerol, 2.0% of 1, 3-butanediol, 0.025% of lactic acid and the balance of purified water.

0.5% of chitosan quaternary ammonium salt, 0.22% of polyhexamethylene biguanide hydrochloride, 3.0% of hydroxypropyl methylcellulose, 2.0% of trehalose, 1.5% of amino acid humectant, 2.0% of fish collagen peptide, 5.0% of witch hazel distillate, 3.0% of aloe barbadensis extract, 4.0% of glycerol, 3.0% of 1, 3-butanediol, 0.03% of acetic acid and the balance of purified water.

1.5% of chitosan quaternary ammonium salt, 0.16% of chlorhexidine acetate, 1.0% of hydroxyethyl cellulose, 2.0% of trehalose, 600.6% of MG, 1.5% of fish collagen peptide, 4.0% of witch hazel distillate, 3.0% of aloe barbadensis extract, 3.0% of glycerol, 2.0% of 1, 3-butanediol, 0.02% of lactic acid and the balance of purified water.

On the basis of the scheme, the preparation method of the ocean refined gynecological bacteriostatic gel comprises the following steps:

(1) taking a required amount of purified water, adding a weighed humectant, and stirring to completely dissolve the purified water to obtain a solution A;

(2) adding glycerol and 1, 3-butanediol into the solution A according to the required amount, uniformly mixing, slowly adding the gel matrix and the chitosan quaternary ammonium salt under stirring, rapidly stirring for 10min to uniformly disperse, gradually heating to 55 +/-5 ℃, continuously stirring under heating to obtain uniform transparent gel B, and stopping heating;

(3) on the basis of the gel B, adding a cell repair protective agent, a plant extracting solution and a bacteriostatic agent according to required amounts, finally adding a pH regulator to regulate the pH value, fully stirring and uniformly mixing, standing and defoaming to obtain the uniform transparent marine refined gynecological bacteriostatic gel.

The invention has the beneficial effects that:

1) the chitosan quaternary ammonium salt is a water-fast-dissolving cationic chitosan high-grade derivative prepared by chemically modifying marine organism chitosan, is a cationic polymer, and can destroy the cell membrane of the marine organism chitosan after bacteria are adsorbed, so that DNA and RNA in cytoplasm flow out to achieve the aim of killing the bacteria. The antibacterial property of the product is superior to that of chitosan and other chitosan derivatives, and especially the product shows better antibacterial property in the weakly acidic marine refined gynecological antibacterial gel.

2) The formula of the marine essence is fused, safe and effective antibacterial components are cooperated to achieve long-acting bacteriostasis, and the vagina cleaning agent is mild, free of stimulation and capable of purifying the vagina environment; the sea active ingredients are used for replacing traditional Chinese medicines, mint, borneol and the like, so that the sea active ingredients are safer and more comfortable, and bring pleasant and comfortable feeling.

3) The sea cucumber skin care cream is rich in chitosan, fish collagen peptide and marine refined nourishing elements, and active ingredients such as trehalose, an amino acid humectant, an aloe barbadensis extract, witch hazel distillate, MG-60 and the like are fused, so that the skin care cream is effective in moisturizing, deeply protects, repairs and activates cells, helps repair private mucosa, and makes private skin moist, fresh and healthy.

4) The pH value of the private part is balanced, a slightly acidic environment is kept, infection discomfort and peculiar smell caused by flora imbalance are effectively prevented, and the protection capability of the private part skin to the outside is improved; meanwhile, an invisible film can be formed to protect the vaginal mucosa from being invaded by new external pathogenic microorganisms, and the vaginal mucosa is maintained deeply.

Detailed Description

Terms used in the present invention have generally meanings as commonly understood by one of ordinary skill in the art, unless otherwise specified.

The present invention will be described in further detail with reference to the following data in conjunction with specific examples. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.

Example 1

The preparation method of the ocean refined gynecological bacteriostatic gel comprises the following steps of:

(1) taking a required amount of purified water, adding trehalose and an amino acid humectant which are weighed according to the amount, and stirring to completely dissolve the trehalose and the amino acid humectant to obtain a solution A;

(2) adding glycerol and 1, 3-butanediol into the solution A according to the required amount, uniformly mixing, slowly adding hydroxyethyl cellulose and chitosan quaternary ammonium salt under stirring, rapidly stirring for 10min to uniformly disperse, gradually heating to 55 +/-5 ℃, continuously stirring under heating to obtain uniform transparent gel B, and stopping heating;

(3) adding fish collagen peptide, MG-60, Hamamelis mollis distillate, Aloe Barbadensis extractive solution, and polyhexamethylene biguanide hydrochloride into the gel B, and adding lactic acid to adjust pH. Fully stirring and uniformly mixing, standing and defoaming to obtain the uniform transparent marine refined gynecological bacteriostatic gel.

Example 2

The preparation method is the same as example 1.

Example 3

The preparation method is the same as example 1.

Comparative example 1

1.0% of chitosan quaternary ammonium salt, 0.26% of polyhexamethylene biguanide hydrochloride, 1.0% of hydroxyethyl cellulose, 1.0% of trehalose, 1.0% of amino acid humectant, 1.0% of fish collagen peptide, 5.0% of witch hazel distillate, 5.0% of aloe barbadensis extract, 3.0% of glycerol, 2.0% of 1, 3-butanediol, 0.025% of lactic acid and the balance of purified water.

The preparation method is the same as example 1.

Comparative example 2

1.0% of chitosan quaternary ammonium salt, 0.26% of polyhexamethylene biguanide hydrochloride, 1.0% of hydroxyethyl cellulose, 1.0% of trehalose, 1.0% of amino acid humectant, MG-600.6%, 5.0% of witch hazel distillate, 5.0% of aloe barbadensis extract, 3.0% of glycerol, 2.0% of 1, 3-butanediol, 0.025% of lactic acid and the balance of purified water.

The preparation method is the same as example 1.

Comparative example 3

1.0% of chitosan quaternary ammonium salt, 0.26% of polyhexamethylene biguanide hydrochloride, 1.0% of hydroxyethyl cellulose, 1.0% of trehalose, 1.0% of amino acid humectant, MG-600.6%, 1.0% of fish collagen peptide, 5.0% of aloe barbadensis extract, 3.0% of glycerol, 2.0% of 1, 3-butanediol, 0.025% of lactic acid and the balance of purified water.

The preparation method is the same as example 1.

Comparative example 4

1.0% of chitosan quaternary ammonium salt, 0.26% of polyhexamethylene biguanide hydrochloride, 1.0% of hydroxyethyl cellulose, 1.0% of trehalose, 1.0% of amino acid humectant, MG-600.6%, 1.0% of fish collagen peptide, 5.0% of witch hazel distillate, 3.0% of glycerol, 2.0% of 1, 3-butanediol, 0.025% of lactic acid and the balance of purified water.

The preparation method is the same as example 1.

Example 4

Detecting the pH value and the microbial limit:

the pH and microbial limit tests were carried out on the oceanic refined gynecological bacteriostatic gels prepared in examples 1-3 according to the disinfection technical specification (2002 edition), and the test results are shown in table 1:

TABLE 1pH value, microbial Limit test results

The pH value and the microbial limit detection are carried out according to the disinfection technical specification (2002 edition), and the results show that the pH value and the microbial limit of the ocean refined gynecological bacteriostatic gel prepared in the examples 1-3 meet the standard requirements.

Example 5

Bacteriostatic test of the sea refined gynecological bacteriostatic gel:

washing the tested bacteria 24h slant culture to prepare bacterial suspension with proper concentration for preparing bacterial tablets. Spreading the sterilized carrier tablet in a sterile plate, dripping 10 μ L of bacterial suspension one by one, uniformly coating the whole carrier surface with an inoculating ring, and drying in a 37 deg.C incubator. The bacterial tablets are taken, respectively added into and soaked in 5.0g of the ocean refined gynecological bacteriostatic gel prepared in the examples 1-3 and 5.0g of the gel prepared in the comparative examples 1-4, timing is started, the bacterial tablets are respectively acted for 2 min, 5 min, 10min and 20min, the bacterial tablets are taken out by using sterile forceps and are put into a test tube containing 5.0ml PBS, the mixture is fully mixed, and the mixture is properly diluted to be used as a test group. Adding another 1 piece of the bacterial tablet directly into a 5.0ml PBS test tube, mixing well, and diluting properly to serve as a control group. Sucking 0.5mL of the culture medium respectively, placing the culture medium in two plates, pouring 15mL of nutrient agar culture medium (bacteria) or Sabouraud's agar culture medium (fungi) cooled to 40-45 ℃, rotating the plates to be fully and uniformly, turning over the plates after the agar is solidified, culturing the culture medium for 48h (bacteria) or 72h (fungi) at 35 +/-2 ℃, and counting the bacterial colonies of the viable bacteria. The bacteriostatic performance of the gel sample is evaluated by calculating the bacteriostatic rate, and the calculating method comprises the following steps:

in the formula: x-bacteriostasis rate,%;

N_C-average colony number of control samples;

N_S-average colony number of samples tested.

TABLE 2 inhibitory effect of Marine refined gynecological bacteriostatic gel on test bacteria

The test results in table 2 show that the marine refined gynecological bacteriostatic gel has strong bacteriostatic action on escherichia coli, staphylococcus aureus and candida albicans.

Example 6

Stability test of the sea refined gynecological bacteriostatic gel:

10g of each of the oceanic refined gynecological bacteriostatic gels prepared in the embodiments 1-3 is respectively put into a centrifugal tube with scales, and is centrifuged for 20min at 2500r/min, whether the sample has a layering phenomenon or not is observed, and the result shows that the layering phenomenon does not occur, so that the oceanic refined gynecological bacteriostatic gel has good centrifugal stability.

Taking 5g of each of the ocean refined gynecological bacteriostatic gels prepared in the embodiments 1-3, placing the gels in a constant temperature box at 37 ℃ for 90 days, respectively measuring the contents of main effective components before and after placing, and measuring the bacteriostatic rate after placing by combining the bacteriostatic test in the embodiment 5. The test results of the content of the main effective components and the stability of the antibacterial performance are shown in tables 3 and 4.

TABLE 3 determination results of the contents of main effective components in the ocean extract gynecological antibacterial gel

Inhibiting effect of ocean refined gynecological bacteriostatic gel on test bacteria after being stored in constant temperature box at temperature of 437 ℃ for 90 days

The test results of the centrifugal stability, the content reduction rate of the main effective components and the stability of the bacteriostatic performance show that the ocean refined gynecological bacteriostatic gel has the centrifugal stability and the long-term stability, after the gel is placed in a constant-temperature incubator at 37 ℃ for 90 days, the content reduction rate of the main effective components of the stock solution is less than or equal to 10%, the bacteriostatic rate on escherichia coli, staphylococcus aureus and candida albicans can still reach more than or equal to 90%, the bacteriostatic action is still realized, and the storage period of validity is at least 2 years.

Example 7

Vaginal mucosa irritation test of the sea refined gynecological bacteriostatic gel:

according to the test of vaginal mucosa irritation of 2.3.5 items of disinfection technical specification (2002 edition), the experiment is carried out by using ocean refined gynecological bacteriostatic gel stock solution as test solution, and the irritation effect and strength of the prepared gel on the vaginal mucosa are evaluated.

Each of the ocean refined gynecological bacteriostatic gels randomly divides 6 common-grade New Zealand experimental rabbits into an experimental group and a control group, and each group comprises 3 rabbits. Before the experiment, the vaginal orifice of each animal was checked for secretions, congestion and other lesions and a blunt tip hose (catheter) was connected to a 5mL syringe. Fixing the experimental rabbit on the back, exposing perineum and vaginal orifice, wetting the catheter with the test solution, gently inserting the catheter into the vagina for 4-5 cm, slowly injecting 2mL of the test solution by using a syringe, and gently withdrawing the catheter. The control group was treated with physiological saline in the same manner. And (3) after 24h, killing the animals by adopting an air embolism method, taking out the complete vagina, longitudinally cutting, visually observing whether macroscopic lesions such as bleeding and edema exist or not, selecting two ends and the middle of the vagina to carry out routine paraffin embedding and slicing, and carrying out histopathological observation after HE staining. According to the pathological examination results, the stimulation response of the vaginal mucosa is scored, the vagina of the animals in the experimental group and the vagina of the animals in the control group have no obvious macroscopic pathological changes such as hyperemia, edema and the like, and the histopathological examination results are shown in the following table 5.

TABLE 5 Marine refined gynecological gel vaginal mucosa irritation response score

As can be seen from table 5, the average integral of vaginal mucosa irritation reactions of the experimental groups was 1.44, 1.56, and 1.67, and the average integral of the control group was 1.0, so that the vaginal mucosa irritation indexes of the bacteriostatic gels of the groups of examples 1-3 were 0.44, 0.56, and 0.67, respectively, and the irritation of the marine refined gynecological bacteriostatic gel sample stock solution to vaginal mucosa was evaluated in a graded manner according to the vaginal mucosa irritation intensity.

Example 8

The repairing effect of the ocean refined gynecological bacteriostatic gel on epithelial cells is as follows:

selecting 80 ICR mice with the weight of 18-22 g, adaptively feeding for 3 days, dividing into 8 groups (each half of male and female) at random, unhairing the backs of the ICR mice by using 8% sodium sulfide liquid, feeding sodium pentobarbital through the abdominal cavity after 24 hours, shearing out a circular full-skin incision with the diameter of about l.0cm by using an operation scissors after anesthesia is successful, and disinfecting by using 70% alcohol solution; wherein 1-3 groups of the marine refined gynecological bacteriostatic gel prepared in the embodiment 1-3 are respectively coated on the wound, covered with paraffin absorbent gauze, bandaged with a bandage, and then given with normal saline through the abdominal cavity after the wound; the 4 th to 7 th groups of the marine refined gynecological bacteriostatic gel are respectively prepared according to the comparative examples 1 to 4, and the treatment mode is the same as the above; group 8 was coated with a blank gel (hydroxyethylcellulose 1.0%, glycerol 3.0%, 1, 3-butylene glycol 2.0%, balance purified water).

And (5) observing the wound healing conditions 3, 7, 10, 15 and 21d after treatment respectively, and calculating the healing rate. The results are shown in Table 6:

TABLE 6 comparison of wound healing rates (%) for epithelial cell repair action of ocean refined gynecological gel

Group of	3d	7d	10d	15d	21d
						Example 1	11.56	26.14	55.28	85.69	98.86
Example 2	10.63	25.35	53.66	83.58	97.35
						Example 3	10.08	24.99	53.98	81.87	95.88
Comparative example 1	8.86	22.56	50.58	79.36	93.26
						Comparative example 2	8.29	22.08	50.15	79.09	92.48
Comparative example 3	7.56	21.15	49.97	78.45	91.32
						Comparative example 4	7.67	21.32	49.66	78.29	91.59
Blank control group	4.16	9.26	26.85	59.45	78.65

As can be seen from Table 6, the ocean refined gynecological bacteriostatic gel has a good repairing effect on epithelial cells, can promote wound healing, shows a healing effect obviously superior to that of a control group after 7 days, and basically and completely heals 21 days.

Example 9

The test of the curative effect of the ocean refined gynecological bacteriostatic gel on gynecological diseases comprises the following steps:

160 volunteers aged 18-45 years are selected, wherein 40 cases of chronic cervicitis, 40 cases of bacterial vaginitis, 40 cases of trichomonas vaginitis and 40 cases of moderate cervical erosion are randomly divided into two groups, and each group is respectively provided with 20 cases of chronic cervicitis, 20 cases of bacterial vaginitis, 20 cases of trichomonas vaginitis and 20 cases of moderate cervical erosion; wherein the first group adopts the marine refined gynecological antibacterial gel of the embodiment 1 of the invention, and the second group adopts the commercial antibacterial gel (Wangjin Tinbukang gel); the using method comprises the following steps: after the private part is washed before sleeping every night, the gynecological bacteriostatic gel is pushed into the deep vagina for 1 count every night, and the results are reviewed after one treatment course (7 days), and are shown in table 7:

TABLE 7 therapeutic effect of ocean refined gynecological antibacterial gel on gynecological diseases

As can be seen from table 7, the total effective rate and cure rate of the marine refined gynecological antibacterial gel provided by the invention on chronic cervicitis, bacterial vaginitis, trichomonas vaginitis and moderate cervical erosion are better than those of the commercially available antibacterial gel, the treatment effect on chronic cervicitis and bacterial vaginitis is especially obvious, no adverse reaction occurs in the treatment period, and the whole treatment process is safe and reliable.

The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.

Claims

1. The ocean pure gynecological antibacterial gel is characterized in that: the paint is prepared from the following components in percentage by weight:

1.0% of chitosan quaternary ammonium salt, 0.26% of polyhexamethylene biguanide hydrochloride, 1.0% of hydroxyethyl cellulose, 1.0% of trehalose, 1.0% of amino acid humectant, MG-600.6%, 1.0% of fish collagen peptide, 5.0% of witch hazel distillate, 5.0% of aloe barbadensis extract, 3.0% of glycerol, 2.0% of 1, 3-butanediol, 0.025% of lactic acid and the balance of purified water;

the preparation method of the ocean refined gynecological bacteriostatic gel comprises the following steps:

(3) on the basis of the gel B, adding fish collagen peptide, MG-60, witch hazel distillate, aloe vera extract and polyhexamethylene biguanide hydrochloride according to required amount, finally adding lactic acid to adjust the pH value, fully stirring and mixing uniformly, standing and defoaming to obtain the uniform transparent marine refined gynecological antibacterial gel.

2. The ocean pure gynecological antibacterial gel is characterized in that: the paint is prepared from the following components in percentage by weight:

0.5% of chitosan quaternary ammonium salt, 0.22% of polyhexamethylene biguanide hydrochloride, 3.0% of hydroxypropyl methylcellulose, 2.0% of trehalose, 1.5% of amino acid humectant, 2.0% of fish collagen peptide, 5.0% of witch hazel distillate, 3.0% of aloe barbadensis extract, 4.0% of glycerol, 3.0% of 1, 3-butanediol, 0.03% of acetic acid and the balance of purified water;

(2) adding glycerol and 1, 3-butanediol into the solution A according to the required amount, uniformly mixing, slowly adding hydroxypropyl methylcellulose and chitosan quaternary ammonium salt under stirring, rapidly stirring for 10min to uniformly disperse, gradually heating to 55 +/-5 ℃, continuously stirring under a heating condition to obtain uniform transparent gel B, and stopping heating;

(3) on the basis of the gel B, adding fish collagen peptide, witch hazel distillate, aloe barbadensis extract and polyhexamethylene biguanide hydrochloride according to required amount, finally adding acetic acid to adjust the pH value, fully stirring and uniformly mixing, standing and defoaming to obtain the uniform transparent marine refined gynecological antibacterial gel.

3. The ocean pure gynecological antibacterial gel is characterized in that: the paint is prepared from the following components in percentage by weight:

1.5% of chitosan quaternary ammonium salt, 0.16% of chlorhexidine acetate, 1.0% of hydroxyethyl cellulose, 2.0% of trehalose, 600.6% of MG, 1.5% of fish collagen peptide, 4.0% of witch hazel distillate, 3.0% of aloe barbadensis extract, 3.0% of glycerol, 2.0% of 1, 3-butanediol, 0.02% of lactic acid and the balance of purified water;

(1) taking required amount of purified water, adding weighed trehalose, and stirring to completely dissolve the trehalose to obtain a solution A;

(3) on the basis of the gel B, adding fish collagen peptide, MG-60, witch hazel distillate, aloe vera extract and chlorhexidine acetate according to required amount, finally adding lactic acid to adjust the pH value, fully stirring and mixing uniformly, standing and defoaming to obtain the uniform transparent marine refined gynecological antibacterial gel.