CN108918626B - Concentration response type biochemical multi-component online analyzer - Google Patents
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Abstract
The invention discloses a concentration response type biochemical multi-component online analyzer, which comprises a multi-component concentration detection module, an accurate sample introduction mechanical arm, a multi-channel data acquisition module, an automatic sampling module and a multi-channel flow path structure, wherein the accurate sample introduction mechanical arm is connected with the multi-channel data acquisition module; the multi-component concentration detection module consists of a detection pool, a sample pool, a sampling pool, a cleaning pool and a waste liquid pool, and can realize the on-line detection of the concentration of various components; the sample introduction mechanical arm consists of a two-degree-of-freedom mechanical arm, a precision injector and a sample introduction needle, and realizes the precise positioning sample introduction control of the detection cell; the multi-channel data acquisition module consists of an embedded development board and a touch screen, and realizes the on-line detection, display and storage of the concentration of various components; the automatic sampling module consists of a precision membrane sampler, a miniature vacuum diaphragm pump and a stop valve; the multi-channel flow path integrated structure is formed by connecting and assembling four parts. The invention can realize the on-line real-time detection of the concentration of various substrates, key intermediate metabolites and products in the microbial fermentation process, has compact structure and is convenient for batch production.
Description
Technical Field
The invention belongs to the crossing field of biosensing technology and industrial automation technology, and particularly relates to a multi-component concentration online analyzer for realizing multi-component concentration response in a microbial fermentation process based on a biosensing electrode.
Background
The fermentation process mainly relates to the growth and metabolism of microorganisms, and the types, concentrations and interactions of substrates, key intermediate metabolites and products in a fermentation system have great influence on the reaction rate, the product yield and the selection of metabolic pathways by cells in the microbial fermentation process. The regulation and control aim to the fermentation process is to regulate the factors such as environment and the like to the state which is most suitable for the growth of microorganisms, and the relevant information of environmental conditions and microorganism physiology must be known in the regulation and control process. Compared with the conventional detection aiming at the parameters of environmental conditions such as pH, dissolved oxygen, temperature and the like, the detection of the key parameters of the multi-component concentration related to fermentation parameters and conditions is very important, the information provided by the detection can directly reflect the growth process of microorganisms, and is beneficial to better understanding of the fermentation process by people, so that the technological process is improved, the online detection and feedback control of the components of the substrate and the product in the fermentation process are realized, the stable operation of the fermentation process can be favorably ensured, and the fermentation efficiency is improved.
The analysis and quantitative detection of the concentration of the component components in the current fermentation process mainly comprises the following methods: chemochromic/titration, chromatography, electrochemical-biosensor methods, and the like. The detection methods of the former two instruments have long operation period and narrow linear range, and are not suitable for online detection. These methods have been widely used to obtain information about a part of the fermentation process by off-line sampling and detection, and thus to guide the regulation of the fermentation process. The electrochemical-biosensor method uses bioactive substances (such as enzymes, cells, antibodies, aptamers, and the like) as biological functional sensitive elements and fixes the bioactive substances on a signal converter, and a target substance to be detected can perform a specific reaction (such as an enzyme catalytic reaction) with a loaded bioactive substance and is converted into a corresponding electrical signal (such as potential, current, resistance or capacitance) through the signal converter, and the analog-to-digital conversion is realized by matching with an embedded microelectronic technology, so that the electrical signal is converted into a digital quantity which can be recognized by equipment, and thus, quantitative or qualitative analysis and detection of the target substance to be detected are realized. Therefore, the kit has the advantages of good selectivity, high sensitivity and the like for target objects to be detected in a complex component system, is rich and adjustable in size and style, and is very suitable for being used in a microbial fermentation environment. Because the sensitivity is high, the miniaturization is easy, the detection can be carried out in a complex system sample, and the like, the method is more favored by the fermentation process.
With the development of fermentation engineering, the requirements on the real-time performance, integrity and reliability of important fermentation parameters of information are increasingly raised. Most of the existing component concentration analysis instruments on the current market adopt offline sampling and dilution, manual sampling is needed, and the low-concentration component concentration is subjected to offline detection through complex pretreatment, while the component concentration in a fermentation system is often far higher than the detection linear range of the instruments, so that the sample must be diluted to carry out detection offline detection, the detection period is greatly prolonged, and detection data cannot reflect the component concentration change (such as substrates, key intermediate metabolites and products) in the current fermentation system in time, so that the fermentation process cannot be guided and regulated in time, and the wide application of a fermentation process control technology based on concentration feedback is greatly limited.
Therefore, the component concentration online analyzer can provide real-time component concentration change information for the fermentation process due to the advantages of the component concentration online analyzer in real time and reliability compared with the existing offline concentration analyzer, so that a related feedback control loop is established, the automatic control of the fermentation process is realized, and the component concentration online analyzer has wide application prospects.
Disclosure of Invention
The invention aims to provide an on-line concentration analyzer capable of realizing multi-component on-line detection by adopting a biosensing electrode aiming at the problem of lacking of an on-line detection technology of substrate, important metabolic intermediate and product components in the fermentation process.
In order to achieve the purpose, the invention adopts the technical scheme that: a concentration response type biochemical multi-component online analyzer comprises a multi-component concentration detection module, a precise sample introduction mechanical arm, a multi-channel data acquisition module, an automatic sampling module and a multi-channel flow path structure;
the multi-component concentration detection module comprises a detection pool, a sample pool, a sampling pool, a cleaning pool and a waste liquid pool, wherein biosensing electrodes for detecting different components are fixedly arranged on the detection pool, and the on-line detection of the concentration of the different components is realized by collecting current response signals of the biosensing electrodes;
the precise sample injection mechanical arm comprises a two-degree-of-freedom mechanical arm, a precise injector and a sample injection needle, wherein one end of the precise injector is connected with the two-degree-of-freedom mechanical arm, the sample injection needle is arranged below the other end of the precise injector, and the two-degree-of-freedom mechanical arm drives the precise injector and the sample injection needle to realize precise sampling from a sampling pool or a standard sample pool and precise and quantitative injection to a detection pool through free rotation positioning in the horizontal direction and precise positioning control of up-and-down movement in the vertical direction;
the multi-channel data acquisition module comprises an embedded development board and a touch screen and is used for realizing multi-component concentration online detection, display and storage; the method comprises the steps of reading weak current signals of sensing electrodes of different detection cells, amplifying and converting the weak current signals into standard digital quantity signals, and realizing online detection, display and storage of concentrations of various components;
the automatic sampling module comprises a precise membrane sampler, a miniature vacuum diaphragm pump and a stop valve, quantitatively samples from the fermentation tank at regular time by adopting the stop valve and the vacuum diaphragm pump, intercepts and separates biological cells in sample liquid by the precise membrane sampler, avoids the pollution of the biological cells on a pipeline, a detection electrode and a detection tank, and sends the separated sample liquid to the sampling tank for multi-component concentration online detection;
the multi-channel flow path structure is used for connecting the multi-component concentration detection module, the accurate sample introduction mechanical arm, the multi-channel data acquisition module and the automatic sampling module, and integrating and assembling all the parts into a whole.
Furthermore, the multi-component concentration detection module takes a disc as a bottom support, the two-degree-of-freedom mechanical arm is arranged at the center of the disc, a needle head of a precision injection pump is fixed at the tail end of the mechanical arm, and the detection pool, the sample pool, the sampling pool, the cleaning pool and the waste liquid pool are arranged at the periphery of the disc.
Furthermore, the number of the detection pools is 3, namely a detection pool A, a detection pool B and a detection pool C, the number of the sample pools is 6, and the number of the sampling pool, the number of the cleaning pool and the number of the waste liquid pool are respectively one; detect pond A, detect pond B, detect pond C, wash pond, waste liquid pond, sample pond, 6 standard sample ponds and set up along the disc edge clockwise in proper order, and each function pond center need fix and use the disc center as the centre of a circle, and the radius is on a concentric circle of Rcm, and the arm of being convenient for realizes advancing the kind to the accuracy of all function ponds.
Furthermore, the embedded development board is connected with the sensing electrodes in the detection cell A, B, C through plug-and-play signal lines, and the embedded development board is connected with the touch screen through a standard 485 line or a TCP/IP protocol network port to provide a data standard interface, so that data remote transmission, display and storage are realized.
Furthermore, a precision membrane sampler, a miniature vacuum diaphragm pump and a stop valve in the multi-channel data acquisition module are integrated into a whole and fixed on one side of the instrument shell; the stop valve and the miniature vacuum diaphragm pump are used for sampling quantitatively from the fermentation tank at regular time, cells in the sample liquid are intercepted and separated through a special membrane material in the sampler, so that the pollution of the cells to a pipeline, a detection electrode and a detection pool is avoided, and the separated sample liquid is input into the middle pool to be convenient for the extraction of the sample pool.
Furthermore, the analyzer is made of a mould made of PVC, the shell main body is a cuboid, the middle part of the shell main body is hollow, and the shell main body can be detached; transparent glass doors are adopted on the upper half part and the left side surface of the front surface of the analyzer, so that observation and maintenance in the instrument testing process are facilitated; a touch screen is arranged right above the instrument shell, so that man-machine interaction is facilitated; the left side of the instrument shell is connected with the automatic sampling module, and the right side of the instrument shell is provided with the tray for placing the label liquid tank, the buffer liquid tank and the waste liquid tank.
Further, instrument middle part cavity is cut apart with the panel, and multicomponent concentration detection module, accurate appearance arm, multichannel data acquisition module are placed in proper order on upper portion, 8 accurate peristaltic pumps, one-way stop valves are fixed to the bottom to carry out the pipe connection through the silica gel hose.
To sum up, the structure design of the concentration response type biochemical multi-component online analyzer mainly comprises: (1) the method comprises the steps of (1) designing a multi-component concentration detection module, (2) designing a multifunctional accurate sample introduction mechanical arm, (3) designing a multi-channel data acquisition module, (4) designing an automatic sampling module structure, (5) designing a multi-channel flow path structure.
The analyzer comprises the following specific steps:
step 1: and (3) designing the structure of the multi-component concentration sensing module. The disc is used as a bottom support, and a plurality of detection pools (such as a detection pool A, a detection pool B, a detection pool C and the like), a cleaning pool, a waste liquid pool, a sampling pool and a plurality of standard sample pools (such as a standard sample pool A, a standard sample pool B, a standard sample pool C and the like) are sequentially fixed clockwise along the edge. The multifunctional accurate sample introduction mechanical arm is fixed at the center of the disc, the circle centers of all the functional pools (the detection pool, the cleaning pool, the waste liquid pool, the sampling pool and the standard sample pool) are fixed on a center circle at the center of the disc, and the mechanical arm is convenient to accurately introduce samples.
Step 2: design of multifunctional precision sample introduction mechanical arm. The automatic quantitative sampling device is composed of a servo motor, a mechanical arm, a precise injector, a support and a needle head, wherein the mechanical arm can realize 360-degree free rotation precise positioning control, can realize vertical movement precise positioning control, realizes precise quantitative sampling from a sampling pool (or a standard sample pool) by driving the precise injector, and injects the sample into a corresponding detection pool for full-automatic program control.
And step 3: and designing a multi-channel data acquisition module. The device consists of an embedded development board and a touch screen, wherein the touch screen is externally arranged above the instrument, provides a human-computer interface and a data interface, and is convenient to operate. The embedded development board is arranged on a back board of the analysis instrument, on one hand, multi-channel data acquisition of multi-component concentration response weak current signals, signal amplification and standard digital signal conversion are realized, and on-line detection, display and storage of various component concentrations are realized; on one hand, the automatic program control of all relevant auxiliary equipment such as peristaltic pumps, servo motors and the like in the analytical instrument can be realized through a hardware structure and a software program.
And 4, step 4: and (5) designing the structure of the automatic sampling module. Fixed on the left side of the shell of the analytical instrument and respectively composed of a precise membrane sampler, a one-way stop valve, a miniature vacuum diaphragm pump, a precise peristaltic pump, a waste liquid tank and a middle tank. The method adopts a stop valve and a miniature vacuum diaphragm pump to quantitatively sample from a fermentation tank at regular time, and intercepts and separates cells in the sample liquid through a precise membrane material in a sampler, so that the pollution of the cells to a pipeline, a biological sensing electrode and a detection tank is avoided, and the automatically obtained sample liquid is input into a middle tank to provide sufficient sample liquid for the sampling tank.
And 5: and designing an instrument assembling integrated structure. The main body is designed into a cuboid structure. The upper half part of the front side and the left side surface adopt transparent glass door design, so that the observation and the maintenance of the instrument testing process are facilitated. The middle cavity of the instrument is divided by a panel, as shown in figure 1, a multi-component sensing module, a multifunctional accurate sample introduction mechanical arm and a multi-channel data acquisition module are sequentially arranged at the upper part, and 8 accurate peristaltic pumps are fixed at the bottom. An external touch screen is arranged right above the instrument shell, so that man-machine interaction is facilitated. The left side of the instrument shell is connected with the automatic sampling module, and the right side of the instrument shell is provided with a tray for placing standard liquid, buffer solution and the like.
The invention has the following functions and beneficial effects: the concentration on-line analyzer of the invention utilizes the specificity of enzyme catalytic reaction to detect corresponding biochemical components by the biosensing electrodes loaded with different detection enzymes, thus the analyzer can realize the quantitative detection of the concentrations of various components.
In addition, the multi-component concentration detection module consists of 3 detection pools, 6 sample pools, 1 sampling pool, 1 cleaning pool and 1 waste liquid pool, and can realize on-line detection of the multi-component concentration; the precise sample introduction mechanical arm consists of a two-degree-of-freedom mechanical arm, a precise injector and a sample introduction needle, and realizes precise positioning sample introduction control on the detection pool; the multi-channel data acquisition module consists of an embedded development board and a touch screen, and realizes the on-line detection, display and storage of multi-component concentration. The automatic sampling module consists of a precision membrane sampler, a miniature vacuum diaphragm pump and a stop valve; the multi-channel flow path integrated structure links and assembles the four parts through auxiliary circuits such as peristaltic pumps, electromagnetic valves, pipelines and the like. The invention can realize the on-line real-time detection of the concentration of various substrates, key intermediate metabolites and products in the microbial fermentation process, has compact structure and is convenient for batch production.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
FIG. 1 is a diagram of the assembled and integrated structure of the instrument of the present invention.
FIG. 2 is a block diagram of a multi-channel data acquisition module of the present invention.
Fig. 3 is a block diagram of an automatic sampling module according to the present invention.
FIG. 4 is a connection diagram of the multi-channel flow path structure of the present invention.
Fig. 5 is a block diagram of the sensing module and the multifunctional robot arm of the present invention.
Labeled as: the system comprises a 1-multi-component concentration detection module, a 2-precision sample introduction mechanical arm, a 3-multi-channel data acquisition module, a 4-detection pool A, a 5-detection pool B, a 6-detection pool C, a 7-cleaning pool, an 8-waste liquid pool, a 9-sampling pool, 10-6 standard sample pools, an 11-two-degree-of-freedom mechanical arm, a 12-precision injector and a 13-sample introduction needle.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments.
The first embodiment.
As shown in fig. 1 to 5, a concentration response type biochemical multi-component online analyzer comprises a multi-component concentration detection module, a precise sample introduction mechanical arm, a multi-channel data acquisition module, an automatic sampling module and a multi-channel flow path structure.
The specific connections of the modules are as follows:
the internal connection of the multi-component sensing detection module is as follows: the circle centers of all the functional pools (the detection pool, the cleaning pool, the waste liquid pool, the sampling pool and the standard sample pool) are fixed on a center circle of the center of the disc. The cavity of the detection pool A, B, C cell body is designed by an inverted circular truncated cone, different biosensing electrodes are placed in the detection pool, the top of the cell body is a sealing soft plug prepared by silica gel, and a rectangular data acquisition interface (used for connecting the biosensing electrodes with the data acquisition module) and a cylindrical sample inlet (diameter is 1-2mm, which is convenient for the injection of a precision syringe needle into a sample) are respectively arranged on the soft plug. The upper part of the outer side of the detection pool A is provided with a liquid inlet, and the detection pool A is respectively connected with a No. 1 peristaltic pump and a cleaning pool through silica gel hoses, so that buffer liquid can be quantitatively pumped from the cleaning pool to the detection pool; the lower part of the outer side of the tank body is provided with a liquid outlet which is connected with a No. 2 peristaltic pump through a silica gel hose for discharging waste liquid in the tank, thereby completing the calibration, sample introduction and cleaning processes of the detection tank. Adopt this same design structure to detect pool B, C outside upper portion inlet respectively through 2, No. 3 peristaltic pumps with wash the pond and be connected, outside lower part liquid outlet passes through the silica gel hose and links to each other with No. 4 peristaltic pumps for waste liquid in the pond.
The cleaning pool cavity adopts the cylinder structural design, and the volume is 5ml, and cell body outside upper portion sets up the inlet, connects peristaltic pump and external buffer liquid jar respectively through the silica gel hose, and cell body outside lower part sets up the liquid outlet, through silica gel hose and shunt tubes respectively with 1, 2, No. 3 peristaltic pump be connected to the inlet on the detection pond A, B, C outside.
The sampling pool cavity adopts the cylinder structural design, and the volume is 5ml, and the sealed cork of silica gel preparation is adopted at the cell body top, sets up a cylindrical sample connection above the cork, and the precision syringe needle of being convenient for takes a sample. Cell body outside upper portion sets up the inlet, connects peristaltic pump 5 and the inside middle pond of external autosampler respectively through the silica gel hose, and cell body outside lower part sets up the liquid outlet, is connected to the waste liquid pond through silica gel hose and peristaltic pump.
The waste liquid pool cavity adopts the cylindrical structural design, the volume is 5ml, the lower part outside the pool body is provided with a liquid outlet, the peristaltic pump 6 and the external waste liquid tank are respectively connected through a silica gel hose, and the precision syringe cleaning waste liquid is periodically discharged to the external waste liquid tank from the waste liquid pool.
The cavity of the standard sample pool clamping seat is designed into a cylindrical structure, and a plurality of standard sample pool clamping seats are integrally designed into a whole, as shown in figure 5, the standard sample pool clamping seat can be used for placing a plurality of standard 2ml micro-chromatographic sample feeding bottles.
The connection of multi-functional arm: the mechanical arm main part comprises metal support and metal mechanical arm, and inside cavity is used for the arrangement of servo motor and circuit, and the terminal fixed accurate syringe needle of arm, whole arm can realize 360 rotations, but the up-and-down motion simultaneously realizes the selection in selection function pond, the accurate extraction and the smart volume injection of liquid.
The multi-channel data acquisition module is connected with: the multi-component biological sensing detection electrodes are respectively arranged in the detection pool A, B, C, and signals of the sensing detection electrodes are connected with the multi-channel data acquisition module through a plurality of insertion slot connection signal wires, so that the design of a plug-and-play interface is realized. The embedded data acquisition module is connected with the touch screen through a standard 485 line (or a TCP/IP protocol internet port) to realize data remote transmission, display and storage.
Connection of the automatic sampling module: one end of the peristaltic pump is provided with an interface connected with the reactor, the other end of the peristaltic pump is connected with the membrane sampler, and the bottom end of the membrane sampler is connected with the waste liquid pool. The side end is connected with the middle pool, the middle pool is connected with the sampling pool through the peristaltic pump 6 and the hose, and the precise injector at the end of the sampling mechanical arm can be conveniently extracted.
Example two.
Step 1: and (3) designing the structure of the multi-component sensing module. Designing the size of a base supporting disc: the diameter is 25cm, and the thickness is 3 cm; and a detection pool A, a detection pool B, a detection pool C, a cleaning pool, a waste liquid pool, a sampling pool and a standard sample pool are sequentially fixed on the base disc clockwise along the edge. The cavity of the A, B, C cell body of multicomponent detection tank all adopts the design of radius platform, and the intracavity volume is 5ml, and the material adopts organic glass, and its advantage is highly transparent, can observe the inside condition of sample cell, and corrosion-resistant, moisture-proof, insulating properties is good, can not influence electrode work. Different biosensing electrodes are placed in the cell body, a magnetic stirrer is fixed on the lower portion of the cell body, a sealing soft plug prepared from silica gel is used for sealing the top of the cell body, and a rectangular data acquisition interface (used for connecting the biosensing electrodes with the data acquisition module) and a cylindrical sample inlet (with the diameter of 1-2mm and used for injecting a sample into a needle of a precision injector) are respectively arranged on the soft plug.
The upper part of the outer side of the detection pool A is provided with 1 liquid inlet, the detection pool A is respectively connected with a No. 1 peristaltic pump through a silica gel hose and then connected with a cleaning pool, and 4ml of buffer liquid is quantitatively extracted from the cleaning pool to the detection pool through program control; cell body outside lower part sets up 1 liquid outlet, through silica gel hose connection behind No. 4 peristaltic pumps, connects instrument outside waste liquid jar, can be with the interior waste liquid discharge of pond, accessible procedure realization automatic calibration, advance kind, cleaning circuit control. Adopt this same design structure to detect pool B, C outside upper portion inlet respectively through 2, No. 3 peristaltic pumps are connected with the washing pond through reposition of redundant personnel hose, and outside lower part liquid outlet passes through the silica gel hose and links to each other with No. 4 peristaltic pumps for waste liquid in the pond.
The cleaning pool cavity is designed in a cylindrical structure, the volume of the cleaning pool cavity is 5ml, the cleaning pool cavity is made of organic glass, a liquid inlet is formed in the upper portion of the outer side of the pool body, and the cleaning pool cavity is connected with an external buffer liquid tank of the instrument after being respectively connected with a peristaltic pump 5 through a silica gel hose; the cell body outside lower part sets up the liquid outlet, is connected to the inlet on the detection pond A, B, C outside through silica gel hose and shunt tubes respectively with 1, 2, 3 peristaltic pumps, as shown in figure 4.
The sampling pool cavity adopts the cylinder structural design, and the volume is 5ml, and the material adopts organic glass. The top of the tank body adopts a sealing soft plug prepared by silica gel, and a cylindrical sampling port (the diameter is 1-2 mm) is arranged on the soft plug, so that the sampling of a needle head of a precision injector is facilitated; the upper part of the outer side of the tank body is provided with a liquid inlet which is connected with a peristaltic pump 6 through silica gel hoses and then connected with an internal middle tank of an external automatic sampler; the lower part of the outer side of the tank body is provided with a liquid outlet which is connected to a waste liquid tank through a silica gel hose and a peristaltic pump 7.
The waste liquid pool cavity adopts a cylindrical structure design, the volume is 5ml, and the material is organic glass. The lower part outside the pool body is provided with a liquid outlet, the peristaltic pump 8 is respectively connected through a silica gel hose, and then the liquid outlet is connected with an external waste liquid tank of the instrument, so that the cleaning waste liquid of the precision syringe is periodically discharged to the external waste liquid tank from the waste liquid tank.
The standard sample pool clamping seat cavity adopts a cylindrical structural design, and a plurality of standard sample pool clamping seats are subjected to integrated structural design, so that the standard sample pool clamping seat can be used for placing a plurality of standard 2ml micro-chromatography sample injection bottles.
The precision peristaltic pumps 1-8 and the peristaltic pumps belonging to the modules are controlled by electric signals, the rotating speed is adjustable, the forward and reverse rotation is adjustable, and the control signals are provided by a multifunctional data acquisition module. The radius of the peristaltic pump is 3 cm; the flow range is 2ml/min-200 ml/min.
The centers of all the functional pools are fixed on a concentric circle which takes the center of the disc as the center of a circle and has the radius of 10 cm. The center of the disc is fixed with a multifunctional precise sample introduction mechanical arm through a bolt.
Step 2: design of multifunctional precision sample introduction mechanical arm. The device is composed of a servo motor, a bracket, a two-degree-of-freedom mechanical arm, a precision injector and a needle head. The support is fixed with the center of the disc through a bolt, the mechanical arm is supported, the horizontal length of the two-degree-of-freedom mechanical arm is 10cm, and the horizontal 360-degree-of-freedom-rotation accurate positioning control and the up-and-down movement accurate positioning control can be realized; the mechanical arm is connected with and drives the precision injector to realize precision quantitative sampling from the sampling pool (or the standard sample pool) and injection to the corresponding detection pool.
And step 3: and designing a multi-channel data acquisition module. The multi-component biological sensing detection electrode is mainly composed of an embedded development board and a touch screen, the multi-component biological sensing detection electrodes are respectively placed in a detection pool A, B, C, signals of the sensing detection electrodes are connected with a multi-channel data acquisition module through a plurality of insertion groove connection signal wires, and the plug-and-play interface design is achieved. The embedded development board is arranged on a back board of the analysis instrument, on one hand, multi-channel data acquisition of multi-component concentration response weak current signals, signal amplification and standard digital signal conversion are realized, and on-line detection, display and storage of various component concentrations are realized; on one hand, the automatic program control of all relevant auxiliary equipment such as peristaltic pumps, servo motors and the like in the analytical instrument can be realized through a hardware structure and a software program. The touch screen is long in size: 30cm wide: height of 40 cm: 60 cm; and the embedded data acquisition module and the touch screen are connected with the touch screen through a standard 485 line (or a TCP/IP protocol network port) to realize data remote transmission, display and storage.
And 4, step 4: and (5) designing the structure of the automatic sampling module. Fixed on the left side of the shell of the analytical instrument and respectively composed of a one-way stop valve, a miniature vacuum diaphragm pump, a precise diaphragm sampler, a waste liquid tank and a middle tank. Adopt stop valve and miniature vacuum diaphragm pump to sample regularly from the fermentation cylinder ration, the waste liquid pond is connected to the bottom of membrane sampler, and the pond is in the middle of the side connection, can intercept the separation with the cell in the sample liquid through accurate membrane sampler, avoids its pollution to pipeline, detection electrode and detection pond, and the sample liquid of automatic acquisition is inputed to the middle pond from the filter side, provides the sample liquid of capacity for the sample cell.
And 5: and designing an instrument assembling integrated structure. The main part adopts the PVC material design to be a cuboid shell structure, and the size is long: 30cm wide: height of 40 cm: 60 cm. The upper half part of the front side and the left side surface adopt transparent glass door design, so that the observation and the maintenance of the instrument testing process are facilitated. The middle cavity of the instrument is divided by a panel, as shown in figure 1, a multi-component sensing module, a multifunctional accurate sample introduction mechanical arm and a multi-channel data acquisition module are sequentially arranged at the upper part, and 8 accurate peristaltic pumps are fixed at the bottom. An external touch screen is arranged right above the instrument shell, so that man-machine interaction is facilitated. The left side of the instrument shell is connected with the automatic sampling module, and the right side of the instrument shell is provided with a tray for placing a standard liquid tank, a buffer liquid tank, a waste liquid tank and the like.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the scope of the present invention in any way, and all technical solutions obtained by using equivalent substitution methods fall within the scope of the present invention.
The parts not involved in the present invention are the same as or can be implemented using the prior art.
Claims (7)
1. A concentration response type biochemical multi-component online analyzer is characterized by comprising a multi-component concentration detection module, a precise sample introduction mechanical arm, a multi-channel data acquisition module, an automatic sampling module and a multi-channel flow path structure;
the multi-component concentration detection module comprises a detection pool, a standard sample pool, a sampling pool, a cleaning pool and a waste liquid pool, wherein biosensing electrodes for detecting different components are fixedly arranged on the detection pool, and the on-line detection of the concentrations of various components is realized by collecting current response signals of the biosensing electrodes;
different biological sensing electrodes are placed in the detection tank body, a sealing cork made of silica gel is arranged at the top of the detection tank body, a data acquisition interface used for connecting the biological sensing electrodes with a multi-channel data acquisition module interface and a cylindrical sample inlet convenient for injecting a sample by a precision syringe needle are arranged on the cork, at least one detection tank is arranged, one detection tank is a detection tank A, a liquid inlet is arranged at the upper part of the outer side of the detection tank A, the liquid inlet is respectively connected with a No. 1 peristaltic pump and a cleaning tank through silica gel hoses so as to realize quantitative extraction of a buffer liquid from the cleaning tank to the detection tank, a liquid outlet is arranged at the lower part of the outer side of the detection tank A, and the liquid outlet is connected with a No. 4 peristaltic pump and a waste liquid tank through the silica gel hoses and used for discharging the waste liquid in the tank so as to finish the;
the upper part of the outer side of the cleaning pool is provided with a liquid inlet which is respectively connected with a No. 5 peristaltic pump and an external buffer liquid tank through a silica gel hose, the lower part of the outer side of the cleaning pool is provided with a liquid outlet which is respectively connected with each detection pool through a silica gel hose and a flow dividing pipe;
the top of the sampling pool is provided with a sealing soft plug prepared by silica gel, the soft plug is provided with a sampling port, the upper part of the outer side of the sampling pool is provided with a liquid inlet, the liquid inlet is respectively connected with a No. 6 peristaltic pump and an intermediate pool inside an external automatic sampler through a silica gel hose, the lower part of the outer side of the sampling pool is provided with a liquid outlet, the liquid outlet is connected to a waste liquid pool through a silica gel hose and a No. 7 peristaltic pump, and the waste liquid pool periodically discharges cleaning waste liquid of the precision syringe to an external waste liquid tank;
the precise sample injection mechanical arm comprises a two-degree-of-freedom mechanical arm, a precise injector and a sample injection needle, wherein one end of the precise injector is connected with the two-degree-of-freedom mechanical arm, the sample injection needle is arranged below the other end of the precise injector, and the two-degree-of-freedom mechanical arm drives the precise injector and the sample injection needle to realize precise sampling from a sampling pool or a standard sample pool and precise and quantitative injection to a detection pool through free rotation positioning in the horizontal direction and precise positioning control of up-and-down movement in the vertical direction;
the multi-channel data acquisition module comprises an embedded development board and a touch screen and is used for realizing the on-line detection, display and storage of the concentration of various components; the method comprises the steps of reading weak current signals of sensing electrodes of different detection cells, amplifying and converting the weak current signals into standard digital quantity signals, and realizing online detection, display and storage of concentrations of various components;
the automatic sampling module comprises a precise membrane sampler, a miniature vacuum diaphragm pump and a stop valve, quantitatively samples from the fermentation tank at regular time by adopting the stop valve and the vacuum diaphragm pump, intercepts and separates biological cells in sample liquid by the precise membrane sampler, avoids the pollution of the biological cells on a pipeline, a detection electrode and a detection tank, and sends the separated sample liquid to the sampling tank for multi-component concentration online detection;
the multi-channel flow path structure is used for connecting the multi-component concentration detection module, the accurate sample introduction mechanical arm, the multi-channel data acquisition module and the automatic sampling module, and integrating and assembling all the parts into a whole.
2. The concentration-responsive biochemical multi-component online analyzer according to claim 1, wherein the multi-component concentration detection module is supported by a disk as a bottom, the two-degree-of-freedom mechanical arm is arranged at the center of the disk, a needle of a precision syringe pump is fixed at the tail end of the mechanical arm, and the detection pool, the standard sample pool, the sampling pool, the cleaning pool and the waste liquid pool are arranged at the peripheral edge of the disk.
3. The concentration-responsive biochemical multi-component online analyzer according to claim 2, wherein the number of the detection cells is 3, namely a detection cell A, a detection cell B and a detection cell C, the number of the standard sample cells is 6, and the number of the sampling cell, the number of the cleaning cell and the number of the waste liquid cell are respectively one; detect pond A, detect pond B, detect pond C, wash pond, waste liquid pond, sample pond, 6 standard sample ponds and set up along the disc edge clockwise in proper order, and each function pond center need fix and use the disc center as the centre of a circle, and the radius is on a concentric circle of Rcm, and the arm of being convenient for realizes advancing the kind to the accuracy of all function ponds.
4. The concentration response biochemical multi-component online analyzer according to claim 3, wherein the embedded development board is connected to the sensing electrodes in the detection cell A, the detection cell B and the detection cell C through plug-and-play signal lines, and is connected to the touch screen through a standard 485 line or a TCP/IP protocol network port to provide a data standard interface, thereby realizing remote data transmission, display and storage.
5. The concentration-responsive biochemical multi-component online analyzer according to claim 1, wherein the precision membrane sampler, the micro vacuum diaphragm pump and the stop valve in the automatic sampling module are integrated into a whole and fixed to one side of the instrument housing; the stop valve and the miniature vacuum diaphragm pump are started at regular time to quantitatively sample from the fermentation tank, cells in the sample liquid are intercepted and separated through a special precise diaphragm sampler, so that the pollution of the cells to a pipeline, a detection electrode and a detection pool is avoided, and the separated sample liquid is input into the middle pool to be convenient for the extraction of the standard sample pool.
6. The biochemical multi-component online analyzer of claim 1, wherein the analyzer is made of a mold, the material is PVC, the main body of the housing is a cuboid, the middle part of the main body is hollow, and the main body is detachable; transparent glass doors are adopted on the upper half part and the left side surface of the front surface of the analyzer, so that observation and maintenance in the instrument testing process are facilitated; a touch screen is arranged right above the instrument shell, so that man-machine interaction is facilitated; the left side of the instrument shell is connected with the automatic sampling module, and the right side of the instrument shell is provided with the tray for placing the label liquid tank, the buffer liquid tank and the waste liquid tank.
7. The biochemical multi-component online analyzer of concentration response type according to claim 3, wherein the middle cavity of the analyzer is divided by a panel, the multi-component concentration detection module, the precise sample introduction mechanical arm and the multi-channel data acquisition module are sequentially placed on the upper part, 8 precise peristaltic pumps and one-way stop valves are fixed on the bottom part, and the precise peristaltic pumps and the one-way stop valves are connected through silica gel hoses.
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| CN111061179A (en) * | 2019-10-28 | 2020-04-24 | 南京工业大学 | Embedded modular main control system of multi-component online analyzer |
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