CN108872193A - A kind of body fluid testing device based on SERS technology - Google Patents
A kind of body fluid testing device based on SERS technology Download PDFInfo
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- CN108872193A CN108872193A CN201810720967.5A CN201810720967A CN108872193A CN 108872193 A CN108872193 A CN 108872193A CN 201810720967 A CN201810720967 A CN 201810720967A CN 108872193 A CN108872193 A CN 108872193A
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- 238000004416 surface enhanced Raman spectroscopy Methods 0.000 title claims abstract description 33
- 210000001124 body fluid Anatomy 0.000 title claims abstract description 28
- 239000010839 body fluid Substances 0.000 title claims abstract description 28
- 238000012360 testing method Methods 0.000 title claims description 22
- 238000005516 engineering process Methods 0.000 title claims description 21
- 238000005070 sampling Methods 0.000 claims abstract description 105
- 230000003287 optical effect Effects 0.000 claims abstract description 59
- 239000000758 substrate Substances 0.000 claims abstract description 30
- 238000009434 installation Methods 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 239000000523 sample Substances 0.000 claims description 76
- 239000002775 capsule Substances 0.000 claims description 46
- 239000011159 matrix material Substances 0.000 claims description 21
- 239000012528 membrane Substances 0.000 claims description 18
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 16
- 239000010931 gold Substances 0.000 claims description 16
- 229910052737 gold Inorganic materials 0.000 claims description 16
- 238000007689 inspection Methods 0.000 claims description 12
- 238000002627 tracheal intubation Methods 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 9
- 239000008280 blood Substances 0.000 claims description 8
- 210000004369 blood Anatomy 0.000 claims description 8
- 210000002700 urine Anatomy 0.000 claims description 7
- 239000002077 nanosphere Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 239000013013 elastic material Substances 0.000 claims description 4
- 239000007822 coupling agent Substances 0.000 claims description 3
- 238000000354 decomposition reaction Methods 0.000 claims description 3
- 239000005350 fused silica glass Substances 0.000 claims description 3
- 238000001755 magnetron sputter deposition Methods 0.000 claims description 3
- 238000001020 plasma etching Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000010453 quartz Substances 0.000 claims description 3
- 210000004243 sweat Anatomy 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 230000006835 compression Effects 0.000 claims description 2
- 238000007906 compression Methods 0.000 claims description 2
- 238000009738 saturating Methods 0.000 claims 1
- 238000007789 sealing Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 10
- 239000012535 impurity Substances 0.000 abstract description 3
- 208000027418 Wounds and injury Diseases 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract description 2
- 208000014674 injury Diseases 0.000 abstract description 2
- 101000674278 Homo sapiens Serine-tRNA ligase, cytoplasmic Proteins 0.000 description 17
- 101000674040 Homo sapiens Serine-tRNA ligase, mitochondrial Proteins 0.000 description 17
- 102100040516 Serine-tRNA ligase, cytoplasmic Human genes 0.000 description 17
- 238000001069 Raman spectroscopy Methods 0.000 description 13
- 238000000479 surface-enhanced Raman spectrum Methods 0.000 description 8
- 238000001228 spectrum Methods 0.000 description 7
- 239000003814 drug Substances 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 230000002708 enhancing effect Effects 0.000 description 5
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- 238000004458 analytical method Methods 0.000 description 4
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- 239000012491 analyte Substances 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000010183 spectrum analysis Methods 0.000 description 2
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 2
- 208000002249 Diabetes Complications Diseases 0.000 description 1
- 206010012655 Diabetic complications Diseases 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
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- 238000002405 diagnostic procedure Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 230000003836 peripheral circulation Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012858 resilient material Substances 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
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- 210000004881 tumor cell Anatomy 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- 238000002562 urinalysis Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N2021/653—Coherent methods [CARS]
- G01N2021/655—Stimulated Raman
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention belongs to medical detection fields, are related to a kind of body fluid detection device based on Surface enhanced Raman spectroscopy detection technique.Including computer, detection box, detection card and sampler；The slot of installation detection card, laser source, optical splitter, optical filter is respectively arranged on detection box body, detection card includes accommodating cavity, and accommodating cavity lower surface is that SERS enhances substrate；The sampler is capillary needle sampler, including sampler shell and sampling bolt.Detachable and combined function realizes the plug-in combination of a variety of laser sources, spectrometer, optical filter.Sampler is capillary needle sampler, in sampling to the injury very little of body, and realizes minimally invasive sampling, and furthermore capillary needle sampler can be to avoid the impurity of sucking large volume when sampling liquid.
Description
Technical field
The invention belongs to medical inspection fields, are related to a kind of body fluid testing device, and in particular to one kind is based on surface enhanced
The body fluid testing device of Raman spectrum inspection technology.
Background technique
SERS spectra technology has the advantages that high inspection sensitivity and high specific, is widely used in biological tissue, cell
And the research of biomolecule, show great application potential.Such as blood, saliva, urine etc..SERS spectra technology can be examined
Test Peripheral Circulation tumour cell.2009, Liaocheng University compared diabetes patient and normal person using the analysis of SERS spectra technology
The SERS spectra and type-II diabetes of serum and the SERS spectra of diabetic complication, discovery are comprehensive from different diabetes
The patients serum SERS averaged spectrum of disease and type-II diabetes patient are closely similar, illustrate there is certain connection between these diseases
System.SERS can examine drug and and protein-interacting in blood.Furthermore the SERS spectra quantitative testing of blood constituent content
The quantitative measurment of analysis blood constituent can provide important information in the clinical diagnostic process of disease.SERS spectra technical application
In the analytical control saliva of saliva contain a large amount of protein and body metabolism product, many diseases can by saliva at
The analysis divided is diagnosed.The analytical control urinalysis that SERS spectra technology is applied to urine is called " the fluid work of kidney
Inspection " assesses renal function by the product (such as urea, uric acid, kreatinin etc.) of organism metabolism in analysis urine.
200480041168.1 Intel company of application number has invented a kind of to be visited using Raman active or SERS activity
Needle construction test organisms imitate the various methods of the analyte containing protein in the analyte such as body fluid in product.But it is only
Being illustrated from the principle can provide a device that, there is no the specific structures of disclosed device.
Application number 201610317610.3 discloses a kind of based in Surface enhanced Raman spectroscopy technical checking human body fluid
The method of drugs.By the pre-treatment to drugs in human body fluid, using self assembly gold nano sol material to urine, blood,
Drugs in saliva are quickly examined, and the influence of other impurities is avoided, and are applicable in the inspection of drugs on site.The invention does not have
Specific apparatus structure is provided, and its inspection is pre-processed, operated more complicated.
Current is generally used for laboratory stage using the progress body fluid inspection of SERS technology, main still for ready-made
Body fluid carries out the inspection of various complexity, and the instrument needed is complex, it is also necessary to which people trained carries out professional operation, cannot
Realize the application of clinical even family health care.
Summary of the invention
The present invention overcomes disadvantages described above to provide a kind of body fluid testing device based on SERS technology, which may be implemented pair
The SERS of body fluid is examined, to examine the interested ingredient in body fluid.
A kind of body fluid testing device based on SERS technology, including computer, checkbox, check card and sampler；Its
It is characterized in that being provided with controller and USB interface in the checkbox, examines and be respectively arranged with installation check card, laser on box body
Source, optical splitter, optical filter slot, be wherein provided with hard contact 206,207 in laser source and the corresponding slot of optical splitter, swash
Light source hard contact 206,207 corresponding with optical splitter is electrically connected with the controller；Computer passes through the control of USB interface and checkbox
Device connection processed；Check card includes accommodating cavity and intubation, and accommodating cavity upper surface is vitreous silica light-transmitting opening, and accommodating cavity lower surface is
SERS enhances substrate；The sampler is capillary needle sampler, including sampler shell and sampling bolt.
Checkbox includes the whole checkbox matrix in cuboid, and installation check card is provided on checkbox matrix, is swashed
Light source, optical splitter, optical filter slot, wherein installation check card with installation laser source slot in the same of checkbox matrix
The slot of side, installation optical splitter and optical filter is respectively positioned on the bottom of checkbox matrix, is provided with receiving control in checkbox matrix
The cavity of device, controller are arranged in the cavity of the inside of checkbox matrix, and the cavity is corresponding with laser source and optical splitter to be inserted
Slot has communicating passage, and for the connecting line connecting with hard contact to be arranged, the top of shell, USB is arranged in USB interface in channel
The connection of Interface and Controler signal.
The optical channel of connection laser source, optical splitter and check card is provided in checkbox matrix, optical channel is provided with instead
Mirror and semi-transparent semi-reflecting lens are penetrated, the light of laser source transmitting is reflected into semi-transparent semi-reflecting lens through reflecting mirror, reached after semi-transparent semi-reflecting lens
Check card, the light reflected from check card inner surface are then passed through optical filter after semi-transparent semi-reflecting lens reflect and inject optical splitter.
After the light that laser source emits reaches SERS enhancing substrate, since there are SERS to enhance substrate, it can make in detection card
The Raman scattering signal of sample to be tested be greatly enhanced, the Raman diffused light of enhancing passes through after semi-transparent semi-reflecting lens reflect
Optical filter filters out excitation wavelength, reaches optical splitter.Optical splitter is sent data to after the light of collection is carried out spectrum analysis
Controller, data are uploaded to computer later.Computer shows the spectrum that optical splitter is analyzed on a display screen, and by the light
Spectrum is checked with spectrum known in database, so that it may obtain the ingredient and content of sample to be tested.
The laser source is one of 632nm laser source, 514nm laser source, 1064nm laser source；Optical splitter is grating beam splitting
Device or prismatic decomposition device.
Check card includes the whole main body in card-like, and intubation is stretched out from the side of main body, body thickness 3-5mm, side
A length of 10-30mm, main body are made of the JGS1 fused silica material of 0.8-1mm thickness, and inside has accommodating cavity, the accommodating cavity
Bottom surface is that SERS enhances substrate.
The surface enhanced Raman substrate is the nanometer gold substrate of magnetron sputtering, with a thickness of 100 microns.
The surface enhanced Raman substrate is the nanometer gold substrate of the pyramid structure of using plasma etching preparation, gold
Word tower spacing is 100-300nm, and pyramid height is 50-100nm.
The surface enhanced Raman substrate is the gold nanosphere using coupling agent connection on a quartz substrate, gold nanosphere
Diameter is 50-200nm.
Sampler includes in the both ends open sampler shell of cylindrical body, pressure handle, sampling bolt；The one of the sampler shell
End opening, the flange that outside is provided with convenient for finger grip, one end opposite with flange is close using dustproof membrane on sampler shell
Envelope, dustproof membrane can be pierced through by capillary sampling probe group；Sampler shell is internally provided with pressure handle, sampling bolt setting in flange side
In sampler shell, pressing pressure handle can push sampling bolt；
Sampling bolt includes sampling capsule and capillary sampling probe group, and sampling capsule is integrally cylindrical, and sampling capsule is set close to dustproof membrane side
It is equipped with capillary sampling probe group, the side of sampling capsule installation capillary sampling probe group is made of non-elastic material, and sampling capsule is except installation hair
It is made outside the side of thin sampling probe group of elastic material；Capillary sampling probe group is multiple empty micropins, and number is 50-100；It takes
The inside of sample device shell is provided with protrusion close to dustproof membrane side, and protrusion samples the one of capsule installation capillary sampling probe group for stopping
Side prevents sampling capsule from deviating from from sampler shell when moving, while will not influence capillary sampling probe group and piercing out from dustproof membrane；
Pressure handle is pressed when sampling, pressure handle pushes sampling capsule to move downward, and when sampling capsule and projection contacts, sampling capsule cannot continue
Movement, the tip of capillary sampling probe group is pierced through dustproof membrane and is pierced out from sampler shell at this time, continues to press pressure handle, pressure handle is opened
Begin to squeeze sampling capsule, makes to sample bag pressure contracting, intracapsular air will be sampled and be discharged by sampling probe；When pressure handle cannot continue to push
Air is emptied, capillary sampling probe group is made to be pierced into tissue or sample containing body fluid；Loosen pressure handle after piercing, sampling capsule is made in elasticity
With lower expansion, negative pressure is formed, liquid is made to flow into sampling capsule by the pin hole of sampling probe；
The sampling probe is capillary sampling probe, and each a height of 2-5mm of sampling probe, each sampling probe diameter is 50-500 μm.
It is provided with springhole at the top of sampling capsule, springhole can be opened and closed, and intubation can be inserted in the springhole after opening, be made
Sample liquids flow into check card by intubation.
The object that device is examined is blood, urine or sweat.
Beneficial effects of the present invention are：
Raman verifying attachment of the invention has the function of detachable and combination, and a variety of laser sources, spectrometer, optical filtering may be implemented
The plug-in of piece combines, to expand the checking function of instrument；There is plug-in accessory of the invention itself identification to encode, meter
Calculation machine is provided with corresponding identification function, can examine whether the accessory of insertion is required accessory, improves the accuracy of inspection；This
The check card that invention provides designs for plug-in, and inside can be designed to the Raman enhancing substrate of different structure, improves inspection
Range；Sampler is capillary needle sampler, in sampling to the injury very little of body, and realizes minimally invasive sampling, furthermore hair
Fine needle sampler can be to avoid the impurity of sucking large volume when sampling liquid.
Detailed description of the invention
The overall structure diagram of Fig. 1 apparatus of the present invention；
The check card and sampling bolt schematic diagram of Fig. 2 apparatus of the present invention；
Sampler Fig. 3 of the invention samples schematic diagram.
Specific embodiment
It is specifically described below with reference to the structure and usage of Fig. 1-3 pairs of present apparatus.
Embodiment 1
The present invention provides a kind of body fluid testing device based on SERS technology, including computer 1, checkbox 2, check card 3 and
Sampler 4；It is characterized in that being provided with controller 201 and USB interface 202 in the checkbox 2, set respectively on 2 body of checkbox
It is equipped with the slot of installation check card 3, laser source 203, optical splitter 204, optical filter 205, wherein laser source 203 and optical splitter 204
Be provided with hard contact 206,207 in corresponding slot, the hard contact 206,207 corresponding with optical splitter 204 of laser source 203 with
Controller 201 is electrically connected；Computer 1 is connect by USB interface 202 with the controller 201 of checkbox 2；Check card 3 includes accommodating
Chamber 301 and intubation 302,301 upper surface of accommodating cavity are vitreous silica light-transmitting opening, and 301 lower surface of accommodating cavity is that SERS enhances substrate
303；The sampler 4 is capillary needle sampler, including sampler shell 401 and sampling bolt.
Checkbox 2 includes whole be in cuboid checkbox matrix, be provided on checkbox matrix installation check card 3,
Laser source 203, optical splitter 204, optical filter 205 slot, wherein installation check card 3 with installation laser source 203 slot examining
Test the same side of box matrix, the slot of installation optical splitter 204 and optical filter 205 is respectively positioned on the bottom of checkbox matrix, checkbox
The cavity for accommodating controller 201 is provided in matrix, controller 201 is arranged in the cavity of the inside of checkbox matrix, the sky
Chamber slot corresponding with laser source 203 and optical splitter 204 has communicating passage, and channel is for being arranged and hard contact 206,207
The top of shell is arranged in the connecting line of connection, USB interface 202, and USB interface 202 is connect with 201 signal of controller.
The optical channel of connection laser source 203, optical splitter 204 and check card 3 is provided in checkbox matrix, optical channel is set
It is equipped with reflecting mirror 208 and semi-transparent semi-reflecting lens 209, the light that laser source 203 emits is reflected through reflecting mirror 208 into semi-transparent semi-reflecting lens 209,
After semi-transparent semi-reflecting lens 209 reach check card 3, from 3 inner surface of check card reflect light through semi-transparent semi-reflecting lens 209 reflection after again
Optical splitter 204 is injected across optical filter 205.
After the light that laser source 203 emits reaches SERS enhancing substrate, since there are SERS to enhance substrate, detection can be made to block
The Raman scattering signal of interior sample to be tested is greatly enhanced, and the Raman diffused light of enhancing is worn after semi-transparent semi-reflecting lens reflect
It crosses optical filter and filters out excitation wavelength, reach optical splitter.Optical splitter will send data after the light progress spectrum analysis of collection
To controller, data are uploaded to computer later.Computer shows the spectrum that optical splitter is analyzed on a display screen, and should
Spectrum is checked with spectrum known in database, so that it may obtain the ingredient and content of sample to be tested.
The laser source 203 is one of 632nm laser source, 514nm laser source, 1064nm laser source；Optical splitter 204 is light
Grid optical splitter or prismatic decomposition device.
Check card 3 includes the whole main body in card-like, and intubation 302 is stretched out from the side of main body, body thickness 3-
5mm, side length 10-30mm, main body are made of the JGS1 fused silica material of 0.8-1mm thickness, and inside has accommodating cavity 301, institute
The bottom surface for stating accommodating cavity 301 is that SERS enhances substrate 303.
The surface enhanced Raman substrate is the nanometer gold substrate of magnetron sputtering, with a thickness of 100 microns.
The surface enhanced Raman substrate is the nanometer gold substrate of the pyramid structure of using plasma etching preparation, gold
Word tower spacing is 100-300nm, and pyramid height is 50-100nm.
The surface enhanced Raman substrate is the gold nanosphere using coupling agent connection on a quartz substrate, gold nanosphere
Diameter is 50-200nm.
Sampler 4 includes in the both ends open sampler shell 401 of cylindrical body, pressure handle 404, sampling bolt；The sampler
Open at one end, the flange 402 that outside is provided with convenient for finger grip of shell 401, on sampler shell 401 with 402 phase of flange
Anti- one end is sealed using dustproof membrane 403, and dustproof membrane 403 can be by capillary sampling probe group（406）It pierces through；Sampler shell 401
It is internally provided with pressure handle 404 in flange side, sampling bolt is arranged in sampler shell 401, and pressing pressure handle 404, which can push, to be taken
Sample bolt；
Sampling bolt includes sampling capsule 405 and capillary sampling probe group 406, and sampling capsule 405 is whole cylindrical, and sampling capsule 405 is close
403 side of dustproof membrane is provided with capillary sampling probe group 406, and sampling capsule 405 installs the side of capillary sampling probe group 406 by non-resilient
Material is made, and sampling capsule 405 is made in addition to the side of installation capillary sampling probe group 406 of elastic material；Capillary sampling probe group 406
For multiple empty micropins, number is 50-100；The inside of sampler shell 401 is provided with protrusion close to 403 side of dustproof membrane
407, protrusion 407 for stops sample capsule 405 install capillary sampling probe group 406 sides, prevent sample capsule 405 when moving from
Deviate from sampler shell 401, while will not influence capillary sampling probe group 406 and being pierced out from dustproof membrane；
Pressure handle 404 is pressed when sampling, pressure handle 404 pushes sampling capsule 405 to move downward, when sampling capsule 405 is contacted with protrusion 407
When, sampling capsule 405 cannot continue to move, and the tip of capillary sampling probe group 406 pierces through dustproof membrane and from sampler shell at this time
It pierces out, continues pressing pressure handle 404, pressure handle 404 starts to squeeze sampling capsule 405, makes to sample the compression of capsule 405, will sample in capsule 405
Air is discharged by sampling probe；Air is emptied when pressure handle 404 cannot continue and push, contains the piercing of capillary sampling probe group 406
The tissue or sample 5 of body fluid；Loosen pressure handle 404 after piercing, sampling capsule 405 expands under elastic reaction, forms negative pressure, make liquid
Sampling capsule 405 is flowed by the pin hole of sampling probe；
The sampling probe is capillary sampling probe, and each a height of 2-5mm of sampling probe, each sampling probe diameter is 50-500 μm.
The top of sampling capsule 405 is provided with springhole 408, and springhole 408 can be opened and closed, and the springhole 408 after opening can
With insertion intubation 302, sample liquids is made to flow into check card 3 by intubation 302.
The object that device is examined is blood, urine or sweat.
The sampler shell is two sections for can separating up and down, so set, can guarantee be sampled with first
The shell of sampler has enough intensity when extruding, furthermore when bolt taking-up will be sampled by needing, sampler shell can be beaten
It opens, so that sampling bolt be taken out from shell.
Various plug accessories of the invention, such as laser source and optical splitter have different codings, each different accessory
With respective unique encodings, upon connection, computer obtains this coding by controller to realize the identification to accessory.
It is provided with specific inspection software in computer, the inspection to Raman spectrum may be implemented, and prestore various marks in computer
The inspection database of quasi- sample can be supplied to user and compare.
The above, only the preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, it is any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of body fluid testing device based on SERS technology, including computer（1）, checkbox（2）, check card（3）And it takes
Sample device（4）；It is characterized in that the checkbox（2）Inside it is provided with controller（201）And USB interface（202）, checkbox（2）Body
On be respectively arranged with installation check card（3）, laser source（203）, optical splitter（204）, optical filter（205）Slot, wherein laser
Source（203）And optical splitter（204）Hard contact is provided in corresponding slot（206,207）, laser source（203）With optical splitter
（204）Corresponding hard contact（206,207）With controller（201）Electrical connection；Computer（1）Pass through USB interface（202）With inspection
Test box（2）Controller（201）Connection；Check card（3）Including accommodating cavity（301）And intubation（302）, accommodating cavity（301）Upper table
Face is vitreous silica light-transmitting opening, accommodating cavity（301）Lower surface is that SERS enhances substrate（303）；The sampler（4）For capillary needle
Sampler, including sampler shell（401）With sampling bolt.
2. the body fluid testing device according to claim 1 based on SERS technology, it is characterised in that checkbox（2）Including whole
Body is in the checkbox matrix of cuboid, is provided with installation check card on checkbox matrix（3）, laser source（203）, optical splitter
（204）, optical filter（205）Slot, wherein installing check card（3）With installation laser source（203）Slot in checkbox matrix
The same side, install optical splitter（204）With optical filter（205）Slot be respectively positioned on the bottom of checkbox matrix, checkbox matrix
Inside it is provided with receiving controller（201）Cavity, controller（201）It is arranged in the cavity of the inside of checkbox matrix, the sky
Chamber and laser source（203）And optical splitter（204）Corresponding slot has communicating passage, and channel is for setting and hard contact
（206,207）The connecting line of connection, USB interface（202）The top of shell, USB interface are set（202）With controller（201）
Signal connection；
Connection laser source is provided in checkbox matrix（203）, optical splitter（204）And check card（3）Optical channel, optical channel
It is provided with reflecting mirror（208）And semi-transparent semi-reflecting lens（209）, laser source（203）The light of transmitting is through reflecting mirror（208）It reflects into semi-transparent
Semi-reflective mirror（209）, pass through semi-transparent semi-reflecting lens（209）After reach check card（3）, from check card（3）The light of inner surface reflection is through half
Saturating semi-reflective mirror（209）Optical filter is then passed through after reflection（205）Inject optical splitter（204）.
3. the body fluid testing device according to claim 2 based on SERS technology, it is characterised in that the laser source（203）
For one of 632nm laser source, 514nm laser source, 1064nm laser source；Optical splitter（204）For grating beam splitting device or prismatic decomposition
Device.
4. the body fluid testing device according to claim 1 based on SERS technology, it is characterised in that check card（3）Including whole
Body is in the main body of card-like, intubation（302）It is stretched out from the side of main body, body thickness 3-5mm, side length 10-30mm, main body
It is made of the JGS1 fused silica material of 0.8-1mm thickness, inside has accommodating cavity（301）, the accommodating cavity（301）Bottom surface be
SERS enhances substrate（303）.
5. the body fluid testing device according to claim 4 based on SERS technology, it is characterised in that the surface enhanced is drawn
Graceful substrate is the nanometer gold substrate of magnetron sputtering, with a thickness of 100 microns.
6. the body fluid testing device according to claim 4 based on SERS technology, it is characterised in that the surface enhanced is drawn
Graceful substrate is the nanometer gold substrate of the pyramid structure of using plasma etching preparation, and pyramid spacing is 100-300nm, gold
Word tower height degree is 50-100nm.
7. the body fluid testing device according to claim 4 based on SERS technology, it is characterised in that the surface enhanced is drawn
Graceful substrate is the gold nanosphere using coupling agent connection on a quartz substrate, and the diameter of gold nanosphere is 50-200nm.
8. the body fluid testing device according to claim 1 based on SERS technology, it is characterised in that sampler（4）Including being in
The both ends open sampler shell of cylindrical body（401）, pressure handle（404）, sampling bolt；The sampler shell（401）One end open
Mouthful, the flange that outside is provided with convenient for finger grip（402）, sampler shell（401）Upper and flange（402）It adopts opposite one end
Use dustproof membrane（403）Sealing, dustproof membrane（403）It can be by capillary sampling probe group（406）It pierces through；Sampler shell（401）Convex
Edge side is internally provided with pressure handle（404）, sampling bolt setting is in sampler shell（401）It is interior, press pressure handle（404）It can push
Sample bolt；
Sampling bolt includes sampling capsule（405）With capillary sampling probe group（406）, sample capsule（405）It is whole cylindrical, sample capsule
（405）Close to dustproof membrane（403）Side is provided with capillary sampling probe group（406）, sample capsule（405）Capillary sampling probe group is installed
（406）Side be made of non-elastic material, sample capsule（405）Except installation capillary sampling probe group（406）Side outside by elasticity
Material is made；Capillary sampling probe group（406）For multiple empty micropins, number is 50-100；Sampler shell（401）Inside
Close to dustproof membrane（403）Side is provided with protrusion（407）, protrusion（407）For stopping to sample capsule（405）Capillary sampling probe is installed
Group（406）Side, prevent sampling capsule（405）When moving from sampler shell（401）Middle abjection, while will not influence capillary
Sampling probe group（406）It is pierced out from dustproof membrane；
Pressure handle is pressed when sampling（404）, pressure handle（404）Push sampling capsule（405）It moves downward, when sampling capsule（405）With protrusion
（407）When contact, capsule is sampled（405）It cannot continue to move, at this time capillary sampling probe group（406）Tip pierce through dustproof membrane and from
It is pierced out in sampler shell, continues to press pressure handle（404）, pressure handle（404）Start to squeeze sampling capsule（405）, make to sample capsule（405）
Compression, will sample capsule（405）Interior air is discharged by sampling probe；Work as pressure handle（404）It cannot continue to empty air when pushing, make
Capillary sampling probe group（406）It is pierced into tissue or sample containing body fluid（5）；Loosen pressure handle after piercing（404）, sample capsule（405）
It is expanded under elastic reaction, forms negative pressure, liquid is made to flow into sampling capsule by the pin hole of sampling probe（405）；
The sampling probe is capillary sampling probe, and each a height of 2-5mm of sampling probe, each sampling probe diameter is 50-500 μm.
9. the body fluid testing device according to claim 1 based on SERS technology, it is characterised in that sampling capsule（405）Top
Portion is provided with springhole（408）, springhole（408）It can be opened and closed, the springhole after opening（408）Intubation can be inserted（302）,
Sample liquids are made to pass through intubation（302）Flow into check card（3）.
10. the body fluid testing device according to claim 1 based on SERS technology, it is characterised in that the object that device is examined
It is blood, urine or sweat.
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| CN201810720967.5A CN108872193B (en) | 2018-07-04 | 2018-07-04 | A kind of body fluid testing device based on SERS technology |
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| CN115938908A (en) * | 2023-01-09 | 2023-04-07 | 清谱科技(苏州)有限公司 | Spray needle device of quick sample |
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| CN108872193B (en) | 2019-04-19 |
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