CN108823073B - Harvesting device and harvesting method for microalgae cells - Google Patents
Harvesting device and harvesting method for microalgae cells Download PDFInfo
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Abstract
The invention relates to a microalgae cell harvesting device and a microalgae cell harvesting method, wherein the microalgae cell harvesting device comprises a liquid inlet pipe, a filter residue bed, a harvesting bed, a water spray cleaning pipe, a culture solution collecting tank, a algae mud output port, a waste liquid discharge port, a clean water pump, a filter residue collecting tank, a filter residue cleaning rod, a clean water tank, a rotating wheel, a filter residue discharge port, a slag discharge channel, a rotating shaft and the like, wherein the liquid inlet pipe is connected with the filter residue bed, the filter residue bed and the algae mud output port are respectively connected with the harvesting bed, the harvesting bed is connected with the culture solution collecting tank, the water spray cleaning pipe is arranged in the harvesting bed, the filter residue collecting tank is connected with the filter residue bed, the filter residue discharge port is arranged in the filter residue collecting tank, and the rotating shaft is connected with the clean water tank and the rotating wheel. According to the invention, microalgae cells are rapidly collected through the collection bed with the inclination angle continuously changing from 0 degrees to 90 degrees or changing in a gradient manner, so that the microalgae cells are thoroughly separated from the culture solution and sufficiently cleaned, the problem of unsmooth discharge of the microalgae cells is effectively avoided, and the efficient collection of the microalgae cells is realized.
Description
Technical Field
The invention relates to the technical field of microalgae harvesting, in particular to a microalgae cell harvesting device and a microalgae cell harvesting method.
Background
Microalgae is an autotrophic plant widely distributed in natural environment, and not only can synthesize high-added-value compounds such as grease, protein, glyceroglycosides and the like through photosynthesis, but also is considered as one of the most important grain resources in the future of possible human beings by international grain and agriculture organizations due to the characteristics of high photosynthetic efficiency, fast growth, high per mu yield and the like. However, the small volume of the algae cells causes difficult harvesting and high harvesting cost, and becomes one of the difficult problems for restricting the development and large-scale popularization of the microalgae industry. The common microalgae harvesting method mainly comprises flocculation sedimentation, filtration or centrifugal separation, and the like, wherein a flocculating agent is required to be added in the flocculation sedimentation, so that the recycling of the cultivation wastewater is difficult, the pollution and the waste of water resources are caused, and the industrialized microalgae harvesting is difficult to use. The centrifugal separation cost is high, the kinetic energy of high-speed rotation is required, the harvesting energy consumption is extremely high, and the method is mainly used for laboratory small-scale harvesting analysis. Therefore, the filtration is a main microalgae harvesting method, and the main filtration harvesting method at home and abroad is finished through a manually controlled screen device, for example, the spirulina is manufactured into unpowered screening devices such as a flat screen, a pocket screen or an inclined screen by adopting a 300-380 mesh soft screen, and harvesting is realized through a single-stage inclined filter bed or a multi-stage inclined filter bed. This kind of harvesting device, algae liquid like waterfall generally flows through from the inclined plane filter bed, and moisture permeates the filter screen and algae mud adheres to on the guipure, need utilize the washing of rivers, and the device of this kind of structure not only harvesting device area is very big, and construction cost is also higher, needs incessant rivers to wash moreover, prevents that algae mud from piling up and influencing the gathering, and unmanned management degree of difficulty is big. In addition, the algae mud collected by the flat screen or inclined screen platform needs to flow into a collection pool, and needs to be transferred to an algae mud dehydrator or other drying equipment or containers for further processing by using additional power such as a piston pump, so that the process flow is long and the collection efficiency is low. In order to solve the practical problems of low harvesting efficiency and large occupied area of a flat screen or inclined screen device, chinese patent application CN102696340A proposes an algae liquid harvesting and dewatering device with inclined rotary drum filters connected in series, and the core is to drive a multistage rotary drum to rotationally separate algae liquid and algae mud by using a driving device of the rotary drum, so that pumped algae liquid is rotationally moved in the rotary drum filter. Compared with the existing unpowered flat screen, the device has the advantages that a heavy harvesting film platform needs to be driven to rotate by using a high-power driving device, the power consumption for harvesting is greatly increased due to the fact that the film harvesting device is large and clumsy, and compared with the existing unpowered multi-stage flat screen, the device has the technical advantages that the technical advantages are not obvious, but the problem that harvesting is unsmooth due to accumulation of algae mud on the surface of a roller filtering film is solved.
In summary, the existing harvesting device and method for microalgae cells still have the problems of high harvesting cost, low efficiency and the like, so that a new harvesting device for microalgae cells is necessary to be studied.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a microalgae cell harvesting device and a microalgae cell harvesting method. According to the invention, microalgae cells are rapidly collected through the collection bed with the inclination angle continuously changed from 0 degrees to 90 degrees or changed in a gradient manner, so that the microalgae cells are thoroughly separated from the culture solution and are sufficiently cleaned, the problem that the microalgae cells are not smoothly discharged and accumulated in the collection bed is effectively avoided, the collection efficiency of the microalgae cells is greatly improved, and the efficient collection of the microalgae cells is realized.
The invention aims at providing a microalgae cell harvesting device.
The second objective of the present invention is to provide a method for harvesting microalgae cells.
The invention further aims to provide a method for harvesting the spirulina for synthesizing the glyceroglycosides.
In order to achieve the above purpose, the invention specifically discloses the following technical scheme:
first, the present invention provides a harvesting device for microalgae cells, comprising: the filter residue bed and the recovery bed are arranged above the recovery bed, and the central axes of the filter residue bed and the recovery bed are coincident; the filter residue bed is of a truncated cone-shaped structure with the diameter of the lower end face being larger than that of the upper end face, and the side face of the filter residue bed forms a bed surface of a filtering structure, namely the filter residue bed surface, so that large-particle impurities in the mixture of the collected microalgae cells and the culture solution can be filtered conveniently.
The harvesting bed is of a variable-camber horn funnel-shaped structure, the side wall of the harvesting bed forms a bed surface of a membrane hole filtering structure, namely the harvesting bed surface, and the inclination angle alpha of the bed surface relative to the horizontal plane is continuously changed from 0 degree to 90 degrees or is changed in a gradient manner. The microalgae cells and the culture solution which are filtered by the filter residue bed to remove impurities enter the harvesting bed surface, most of the culture solution is filtered out from the membrane holes of the harvesting bed surface in the flowing process, and the microalgae cells are trapped on the bed surface, so that the quick separation of the microalgae cells and the culture solution is realized, the microalgae cells of most of the culture solution are separated to form algae mud, and the algae mud is discharged from the algae mud output port at the bottom of the harvesting bed after being collected.
Preferably, the gradient change of the inclination angle alpha of the recovery bed surface relative to the horizontal plane is divided into 4 gradients: the gradient change is characterized by a slow initial gradient of the bed surface, so that the bed surface separation area is large, the rapid separation of a large amount of culture solution is facilitated, the gradient of the collected bed surface is rapidly increased along with the rapid separation of the culture solution, the bed surface separation area is rapidly contracted, the flow of microalgae cells and the rapid discharge of the collected bed are facilitated, and the microalgae cells are prevented from accumulating.
Further, harvesting device still includes the feed liquor pipe, the feed liquor pipe is the upper end of annular fixed at the bed surface of filter residue bed, and is provided with a plurality of jet directions on the feed liquor pipe towards the cloth liquid hole of filter residue bed, and the balanced distribution of the culture solution that contains the microalgae cell on the bed surface of filter residue bed has been realized in the setting of cloth liquid hole.
Preferably, the filter residue bed surface is a membrane hole filtering structure, and the membrane hole filtering structure is made of a hard or soft filtering material with membrane holes of 10-100 meshes; the filter residue bed with the aperture range can not only enable microalgae cells to pass smoothly, but also filter impurities on the bed surface of the filter residue bed, so that the microalgae cells and the impurities can be effectively separated.
Preferably, the bed surface of the recovery bed is a membrane hole filtering structure, and the membrane hole filtering structure is made of a hard or soft filtering material with 100-800 meshes of membrane holes; the harvesting bed with the aperture range can not only leave microalgae cells on the surface of the harvesting bed, but also can not influence the passing of liquid in the microalgae cells, and can effectively realize the harvesting of the microalgae cells.
Further, the harvesting device also comprises a filter residue collecting tank, a filter residue cleaning rod, a rotating wheel, a filter residue discharging port, a slag discharging channel and a rotating shaft; the filter residue collecting tank is arranged on the periphery of the lower end face of the filter residue bed, is tightly connected with the filter residue bed, the filter residue discharge port is arranged in the filter residue collecting tank, the slag discharge channel is connected with the filter residue discharge port, the filter residue cleaning rod is connected with the rotating shaft and can circumferentially rotate along the filter residue collecting tank under the driving of the rotating shaft, so that impurities filtered by the filter residue bed can be cleaned continuously, and are discharged through the filter residue discharge port and the slag discharge channel in sequence, and the influence of accumulation of the impurities in the filter residue collecting tank on the filtering effect is prevented; and the central axes of the rotating shaft, the filter residue bed and the harvesting bed are coincident.
One end of the rotating shaft is positioned in the harvesting bed, the other end of the rotating shaft penetrates through the filter residue bed and then is connected with the rotating wheel, the central axes of the rotating shaft, the filter residue bed and the harvesting bed are coincident, the rotating wheel is used for providing driving force for the rotating shaft, and the filter residue collecting tank is used for collecting impurities filtered out from microalgae cells and culture solution.
Preferably, the residue discharge outlet is one or more openings of suitable shape to facilitate the discharge of impurities to a designated area.
More preferably, the filter residue discharge outlet is 2 circular holes and is symmetrically distributed on the bottom surface of the filter residue collecting tank, so that the discharge speed and efficiency of filter residues can be effectively improved.
Further, the harvesting device further comprises a cleaning water spray pipe, the cleaning water spray pipe is positioned in the harvesting bed, water spray holes are formed in the cleaning water spray pipe, the jet flow direction of the water spray holes faces the harvesting bed, and an inclination angle is formed between the jet flow direction of the water spray holes and the direction of the bed surface of the harvesting bed or the flowing direction of microalgae cells. The cleaning spray pipe is mainly used for cleaning and flushing foreign matters such as nutrient salts, flora and the like on the surfaces of microalgae cells on the bed surface of the harvesting bed, and flushing the microalgae cells to the microalgae mud output port to be discharged out of the harvesting bed while cleaning the microalgae cells.
Preferably, the inclination angle between the jet direction of the water spraying hole and the flow direction of the harvesting bed surface or the microalgae cells is 45-90 degrees.
Further, the harvesting device further comprises a clear water tank, the clear water tank is arranged outside the harvesting device, the clear water tank is connected with one end of the cleaning water spray pipe, the other end of the cleaning water spray pipe is located in the harvesting bed, clear water in the clear water tank is salt-containing clean water which does not contain salt or contains the same concentration as culture solution, when algae cells containing the glucosinolates are harvested, the salt-containing clean water which contains the same concentration as the culture solution is required to be used as cleaning solution, the glucosinolates in the algae cells are prevented from being secreted into the cleaning solution, and the loss of the glucosinolates is caused.
Further, the axis of rotation is hollow structure, and the one end of axis of rotation is located the bed of gathering inside, and the other end passes the filter residue bed and rotates the wheel and be connected back again with the fresh water tank intercommunication, one end or both ends of washing spray pipe all are located the part intercommunication in the bed of gathering with the axis of rotation, and wash the spray pipe and can rotate along with the axis of rotation to spray out the washing water to the surface of the bed of gathering.
Further, harvesting device still includes support frame, clear water pump, clear water input port, moves sealing member, the clear water tank is fixed on the support frame, is provided with clear water input port on the clear water tank, is connected through moving sealing member between axis of rotation and the clear water tank, clear water input port is connected with the clear water pump.
Preferably, the dynamic sealing component comprises a dynamic sealing structure of a packing seal and a mechanical seal.
Further, harvesting device still includes the culture solution collecting vat, the culture solution collecting vat sets up in the bed surface lower part of harvesting bed for collect the liquid that filters down in the harvesting bed, the algae mud delivery outlet that the lower extreme of harvesting bed formed runs through the bottom surface of culture solution collecting vat, and the position sealing connection of algae mud delivery outlet and culture solution collecting vat bottom contact prevents that the culture solution in the culture solution collecting vat from revealing back again and mixing with little algae cell.
Microalgae cells and culture solution which are filtered by the filter residue bed to remove impurities enter the harvesting bed surface, the culture solution and the microalgae cells are rapidly separated in the flowing process, the microalgae cells are collected and then discharged from an algae mud output port at the bottom of the harvesting bed, and the culture solution enters a culture solution collecting tank through a membrane hole filtering structure on the harvesting bed surface.
Further, the lower part of the culture solution collecting tank is provided with a waste liquid outlet, so that the culture solution in the culture solution collecting tank is conveniently and uniformly discharged after being collected.
Further, the harvesting device further comprises an algae liquid conveying pump, and the liquid inlet pipe is connected with the algae liquid conveying pump and used for conveying microalgae cells and culture liquid into the liquid inlet pipe.
Secondly, the invention provides a method for harvesting microalgae cells, which comprises the following steps:
(1) The microalgae cells and the culture solution are distributed on the bed surface of the filter residue bed, and impurities in the microalgae cells and the culture solution are filtered through a filtering structure of the bed surface of the filter residue bed;
(2) The microalgae cells and the culture solution which are filtered to remove impurities in the step (1) enter the surface of the harvesting bed, so that the culture solution and the microalgae cells are rapidly separated, and the microalgae cells are collected and discharged from an algae mud output port at the bottom of the harvesting bed.
Finally, the invention provides a method for harvesting spirulina for synthesizing glyceroglycosides, which comprises the following steps:
(1) Firstly, spirulina and culture solution are sent into a liquid inlet pipe through an algae solution conveying pump, the spirulina and the culture solution are uniformly distributed on a filter residue bed surface through a liquid distribution hole by the liquid inlet pipe, impurities in the spirulina and the culture solution are filtered through a membrane hole filtering structure on the bed surface, after the impurities are collected in a filter residue collecting tank, the impurities are cleared from a filter residue outlet by a filter residue cleaning rod and enter a filter residue channel, and finally, a harvesting system is cleared, and the spirulina and the culture solution with the impurities filtered out enter a harvesting bed;
(2) The spirulina and the culture solution which are filtered to remove impurities in the step (1) enter the bed surface of the harvesting bed, and the inclination angle alpha of the bed surface relative to the horizontal plane is continuously changed from 0 degree to 90 degrees or is changed in a echelon manner; meanwhile, salt-containing clean water with the same concentration as the culture solution and arranged in the clean water tank enters the cleaning spray pipe after passing through the hollow rotating shaft, and the clean water is sprayed out from the spray holes of the spray cleaning pipe along with the rotation of the rotating shaft to uniformly clean the spirulina on the surface of the harvesting bed, and the jet direction of the spray holes is inclined at an angle of 45-90 degrees with the flowing direction of the spirulina, so that the culture solution and the spirulina are rapidly separated, and the spirulina is discharged from the alga mud output port at the bottom of the harvesting bed after being collected for the next separation and extraction; and the culture solution enters the culture solution collecting tank through the membrane hole filtering structure on the bed surface and is discharged from the waste liquid outlet.
Compared with the prior art, the invention has the beneficial effects that: compared with the traditional recovery bed, the invention adopts the structure of stacking the filter residue bed and the recovery bed up and down, saves the occupied area of equipment arrangement, timely cleans out large-particle impurities filtered by the filter residue bed through the cleaning rod, and improves the capability of the device for treating wastes. In addition, the bed surface structure of the recovery bed adopts a variable-camber horn funnel-shaped structure with the inclination angle being continuously or stepwisely changed from 0 to 90 degrees, and is characterized in that: when microalgae cells and culture solution just enter the bed surface, the gradient of the bed surface is slower, the separation area of the bed surface is larger, the rapid separation of a large amount of culture solution is facilitated, along with the rapid separation of the culture solution, the harvesting bed surface is rapidly contracted, the inclination angle of the bed surface is also continuously increased, the flow and rapid discharge of the microalgae cells from the harvesting bed are facilitated, the microalgae cell accumulation problem is prevented, and the harvesting efficiency is greatly improved.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiments of the application and together with the description serve to explain the application and do not constitute an undue limitation to the application.
FIG. 1 is a schematic diagram of a harvesting device for microalgae cells according to the invention.
Fig. 2 is a schematic diagram of the structure of the filter bed and the recovery bed in example 1.
Fig. 3 is a schematic diagram of the structure of the filter bed and the recovery bed in example 2.
FIG. 4 is a schematic diagram showing the sealing connection of the clean water tank and the rotary shaft filler.
The label designations in the drawings represent: 1-liquid inlet pipe, 2-filter residue bed, 3-recovery bed, 4-water spray cleaning pipe, 5-culture solution collecting tank, 6-algae mud delivery outlet, 7-waste liquid outlet, 8-clear water pump, 9-filter residue collecting tank, 10-filter residue cleaning rod, 11-clear water tank, 12-rotating wheel, 13-filter residue outlet, 14-algae liquid delivery pump, 15-rotating shaft, 16-support frame, 17-dynamic seal component, 18-clear water inlet, 19-slag discharging channel.
Detailed Description
It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
As described in the background art, the existing microalgae cell harvesting device and method still have the problems of high harvesting cost, low efficiency and the like, so the invention provides a microalgae cell harvesting device and harvesting method, and the invention is further described with reference to the accompanying drawings and detailed description.
Example 1
As shown in fig. 1, 2 and 4, a harvesting device for microalgae cells comprises: the device comprises a liquid inlet pipe 1, a filter residue bed 2, a harvesting bed 3, a water spraying cleaning pipe 4, a culture solution collecting tank 5, an algae mud output port 6, a waste liquid discharge port 7, a clean water pump 8, a filter residue collecting tank 9, a filter residue cleaning rod 10, a clean water tank 11, a rotating wheel 12, a filter residue discharge port 13, an algae liquid conveying pump 14, a rotating shaft 15, a supporting frame 16, a dynamic sealing part 17, a clean water input port 18 and a slag discharge channel 19.
The liquid inlet pipe 1 is connected with the algae liquid conveying pump 14, the filter residue bed 2 is of a truncated cone-shaped structure with the diameter of the lower end face larger than that of the upper end face, the side face of the truncated cone forms a bed face (filter bed face) of the filter structure, the liquid inlet pipe 1 is annularly fixed at the upper end of the bed face of the filter residue bed, and liquid distribution holes with jet flow directions facing the filter residue bed 2 are formed in the liquid inlet pipe 1. The filter residue bed 2 has a membrane hole filtering structure and is made of soft filter cloth with 50 meshes of membrane holes.
The filter residue bed 2 is arranged above the harvesting bed 3, the central axes of the filter residue bed 2 and the harvesting bed are coincident, and an algae mud output port 6 is formed at the lower end of the harvesting bed 3; the periphery of the lower end face of the filter residue bed 2 is provided with a filter residue collecting tank 9, the bottom surface of the filter residue collecting tank 9 is provided with a filter residue discharge port 13, and a slag discharge channel 19 is connected with the filter residue discharge port 13.
The filter residue collecting tank 9 is internally provided with a filter residue cleaning rod 10 which is connected with the rotating shaft 15 and can rotate circumferentially along the filter residue collecting tank 9 under the drive of the rotating shaft 15.
The harvesting bed 3 is of a funnel-shaped structure, the side wall of the harvesting bed 3 forms a bed surface (harvesting bed surface) of a filtering structure, the inclination angle alpha of the bed surface relative to the horizontal plane is continuously changed from 0 to 90 degrees, microalgae cells and culture solution for filtering impurities through the filter residue bed 2 enter the bed surface of the harvesting bed 3, the culture solution and the microalgae cells are rapidly separated in the flowing process, the microalgae cells are collected and discharged from an algae mud output port 6 at the bottom of the harvesting bed 3, the culture solution enters a culture solution collecting tank 5 through a membrane hole filtering structure on the bed surface, and the bed surface of the harvesting bed 3 is of a membrane hole filtering structure and is made of membrane hole 300-mesh hard filter cloth.
The water spraying cleaning pipe 4 is arranged in the harvesting bed 3, the rotating shaft 15 is of a hollow structure, the central axis of the rotating shaft 15 coincides with the central axis of the harvesting bed 3, one end of the rotating shaft 15 is positioned in the harvesting bed 3, the water spraying cleaning pipe 4 is communicated with the part of the rotating shaft 15 extending into the harvesting bed 3, the water spraying cleaning pipe 4 is provided with a plurality of water spraying holes, the jet flow direction of the water spraying holes faces the harvesting bed 3, and the jet flow direction of the water spraying holes and the direction of microalgae cells form an inclined angle of 45 degrees; the other end of the rotating shaft 15 is communicated with a clear water tank, the clear water tank 11 is arranged above the rotating shaft 15 and fixed on a supporting frame 16, a clear water input port 18 is arranged on the clear water tank 11, the rotating shaft 15 is connected with the clear water tank 11 through a dynamic sealing component, the clear water input port 18 is connected with a clear water pump 8, and the dynamic sealing component 17 is of a dynamic sealing structure with a packing seal.
One end of the rotating shaft 15 is positioned in the harvesting bed, the other end of the rotating shaft 15 passes through the filter residue bed 2 and then is connected with the rotating wheel 12, the central axes of the rotating shaft 15, the filter residue bed 2 and the harvesting bed 3 are coincident, the rotating wheel 12 provides driving force for the rotation of the rotating shaft 15, clear water in the clear water tank 11 enters the water spraying cleaning pipe after passing through the hollow rotating shaft 15, one end of the cleaning water spraying pipe is communicated with the rotating shaft or both ends of the cleaning water spraying pipe are communicated with the rotating shaft, along with the rotation of the rotating shaft 15, the clear water uniformly cleans microalgae cells on the surface of the harvesting bed after being sprayed out from a water spraying hole of the water spraying cleaning pipe 4, and the microalgae cells are flushed to the algae mud output port 6 to be discharged out of the harvesting bed 3 while the microalgae cells are cleaned.
The culture solution collecting tank 5 is arranged at the lower part of the bed surface of the harvesting bed 3, an algae mud output port 6 formed at the lower end of the harvesting bed 3 penetrates through the bottom surface of the culture solution collecting tank 5, and the algae mud output port 6 is in sealing connection with the contact part of the bottom surface of the culture solution collecting tank 5.
The lower part of the culture solution collecting tank 5 is provided with a waste solution outlet 7, and the culture solution is discharged through the waste solution outlet 7.
Example 2
As shown in fig. 3, a microalgae cell harvesting apparatus is similar to that of example 1, and is different in that: (1) The bed surface of the filter residue bed 2 is made of hard film materials with film holes of 80 meshes.
(2) The bed surface of the harvesting bed 3 is made of soft filter cloth with membrane holes of 100 meshes.
(3) The gradient change of the inclination angle alpha of the bed surface of the recovery bed 3 relative to the horizontal plane is divided into 4 gradients, which are alpha in turn 1 ＝5°、α 2 ＝15°、α 3 ＝45°、α 4 ＝85°。
Example 3
A microalgae cell harvesting apparatus, similar to example 1, differing in that: (1) The bed surface of the filter residue bed 2 is made of a hard film material with film holes of 30 meshes.
(2) The bed surface of the harvesting bed 3 is made of soft filter cloth with 400 meshes of membrane holes.
(3) The jet flow direction of the water spraying holes on the water spraying cleaning pipe 4 is inclined at an angle of 90 degrees with the flowing direction of the algae mud.
(4) The dynamic seal member 17 is a dynamic seal structure of a mechanical seal.
Example 4
A microalgae cell harvesting apparatus, similar to example 1, differing in that: (1) The bed surface of the filter residue bed 2 is made of a soft filter cloth material with membrane holes of 100 meshes.
(2) The bed surface of the harvesting bed 3 is made of soft filter cloth with membrane holes of 600 meshes.
(3) The jet flow direction of the water spraying holes on the water spraying cleaning pipe 4 is inclined at an angle of 60 degrees with the flowing direction of the algae mud.
(4) The dynamic seal member 17 is a dynamic seal structure of a mechanical seal.
Example 5
A microalgae cell harvesting apparatus, similar to example 1, differing in that: (1) The bed surface of the filter residue bed 2 is made of a hard film material with 10 meshes of film holes.
(2) The bed surface of the harvesting bed 3 is made of a soft membrane with membrane holes of 800 meshes.
(3) The jet flow direction of the water spraying holes on the water spraying cleaning pipe 4 is inclined at an angle of 75 degrees with the flowing direction of the algae mud.
(4) The dynamic seal member 17 is a dynamic seal structure of a mechanical seal.
Example 6
A method for harvesting spirulina for synthesizing glyceroglycosides, comprising the following steps:
(1) Firstly, microalgae and culture solution rich in glucosyl in cells are fed into a liquid inlet pipe 1 through an algae solution conveying pump 14, the spirulina and the culture solution are uniformly distributed on the bed surface of a filter residue bed 2 through liquid distribution holes by the liquid inlet pipe 1, impurities in the spirulina and the culture solution are filtered through a membrane hole filtering structure on the bed surface of the filter residue bed 2, after the impurities are collected in a filter residue collecting tank 9, the impurities are cleared from a filter residue outlet 13 by a filter residue cleaning rod 10 and enter a filter residue channel 19, a harvesting system is cleared, and the spirulina and the culture solution with the impurities filtered out enter a harvesting bed 3;
(2) The spirulina and the culture solution which are filtered to remove impurities in the step (1) enter the bed surface of the harvesting bed 3, meanwhile, clear water in a clear water tank enters a water spraying cleaning pipe 4 after passing through a hollow rotating shaft 15, and the clear water is sprayed out from a water spraying hole of the water spraying cleaning pipe 4 along with the rotation of the rotating shaft 15 to uniformly clean the spirulina on the bed surface of the harvesting bed, and the jet flow direction of the water spraying hole forms an inclined angle of 45-90 degrees with the flowing direction of the spirulina, so that the culture solution and the spirulina are rapidly separated, and the spirulina is discharged from an algae mud output port 6 at the bottom of the harvesting bed 3 after being collected for the separation and extraction of the glycerol glucoside in the next step; the culture solution enters the culture solution collecting tank 5 through the membrane hole filtering structure on the bed surface and then enters the culture solution recovery device 12 from the waste liquid outlet 7 so as to carry out recovery treatment on the culture solution, and then is reused for culturing the spirulina after passing through the liquid outlet valve 13.
(2) The spirulina and the culture solution which are filtered to remove impurities in the step (1) enter the bed surface of the harvesting bed, and the inclination angle alpha of the bed surface relative to the horizontal plane is continuously changed from 0 degree to 90 degrees or is changed in a echelon manner; meanwhile, the salt-containing clean water with the same concentration as the culture solution in the clean water tank enters the cleaning water spray pipe after passing through the hollow rotating shaft,
the foregoing description is only of the preferred embodiments of the present application and is not intended to limit the same, but rather, various modifications and variations may be made by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.
Claims (9)
1. A harvesting device of microalgae cells, characterized in that: the harvesting device consists of a filter residue bed and a harvesting bed; the filter residue bed is arranged above the recovery bed, and the central axes of the filter residue bed and the recovery bed are coincident; the lower end of the harvesting bed forms an algae mud output port;
the filter residue bed is of a truncated cone-shaped structure with the diameter of the lower end face larger than that of the upper end face, and the side face of the truncated cone forms a filter residue bed surface of the filter structure;
the harvesting bed is of a variable-curved horn funnel-shaped structure, the side wall of the harvesting bed forms a harvesting bed surface of a filtering structure, and the inclination angle alpha of the harvesting bed surface relative to the horizontal plane is continuously changed from 0 degree to 90 degrees or is changed in a gradient manner;
the harvesting device further comprises a liquid inlet pipe, the liquid inlet pipe is annularly fixed at the upper end of the bed surface of the filter residue bed, and a plurality of liquid distribution holes with jet flow directions facing the filter residue bed are formed in the liquid inlet pipe;
the bed surface of the filter residue bed is a membrane hole filtering structure;
the harvesting bed surface is of a membrane hole filtering structure;
the gradient change of the inclination angle alpha of the harvesting bed surface relative to the horizontal plane is divided into 4 gradients: 5 °, 15 °, 45 °, 85 °;
the harvesting device further comprises a filter residue collecting tank, a filter residue cleaning rod, a rotating wheel, a filter residue discharging port, a slag discharging channel and a rotating shaft; the filter residue collecting tank is arranged on the periphery of the lower end face of the filter residue bed and is tightly connected with the filter residue bed, the filter residue discharge port is arranged in the filter residue collecting tank, the residue discharge channel is connected with the filter residue discharge port, the filter residue cleaning rod is connected with the rotating shaft and can circumferentially rotate along the filter residue collecting tank under the driving of the rotating shaft, one end of the rotating shaft is positioned in the collecting bed, the other end of the rotating shaft penetrates through the filter residue bed and is connected with the rotating wheel, and the central axes of the rotating shaft, the filter residue bed and the collecting bed coincide;
the harvesting device further comprises a cleaning water spray pipe, the cleaning water spray pipe is positioned in the harvesting bed, a water spray hole is formed in the cleaning water spray pipe, the jet flow direction of the water spray hole faces the harvesting bed, and the jet flow direction of the water spray hole is inclined with the direction of the bed surface of the harvesting bed or the flowing direction of microalgae cells;
the harvesting device further comprises a support frame, a clean water pump, a clean water input port and a dynamic sealing component, wherein the clean water tank is fixed on the support frame, the clean water input port is arranged on the clean water pump, and the clean water pump is connected with the clean water input port;
the harvesting device further comprises a culture solution collecting tank, the culture solution collecting tank is arranged at the lower part of the bed surface of the harvesting bed, an algae mud output port formed at the lower end of the harvesting bed penetrates through the bottom surface of the culture solution collecting tank, and the algae mud output port is in sealing connection with the contact part of the bottom surface of the culture solution collecting tank, so that the culture solution in the culture solution collecting tank is prevented from being mixed with microalgae cells after being leaked.
2. The microalgae cell harvesting apparatus of claim 1, wherein: the bed surface of the filter residue bed is made of a hard or soft membrane material with membrane holes of 10-100 meshes.
3. The microalgae cell harvesting apparatus of claim 1, wherein: the harvesting bed surface is made of a hard or soft membrane material with 100-800 meshes.
4. The microalgae cell harvesting apparatus of claim 1, wherein: the harvesting device further comprises a clean water tank, the clean water tank is arranged outside the harvesting device, the clean water tank is connected with one end of a cleaning water spray pipe, and the other end of the cleaning water spray pipe is located in the harvesting bed.
5. The microalgae cell harvesting apparatus of claim 1, wherein: the axis of rotation is hollow structure, and the one end of axis of rotation is located the bed of gathering inside, and the other end passes the filter residue bed and rotates the wheel and be connected the back again with the clean water tank intercommunication, one end or both ends of wasing the spray pipe all are located the part intercommunication in the bed of gathering with the axis of rotation, and wasing the spray pipe and can rotate along with the axis of rotation to spray out the washing water to the bed surface of gathering.
6. The microalgae cell harvesting apparatus of claim 1, wherein: the inclination angle between the jet flow direction of the water spray hole and the flowing direction of the microalgae cells is 45-90 degrees;
the dynamic sealing component comprises a dynamic sealing structure of a packing seal and a mechanical seal.
7. The microalgae cell harvesting apparatus of claim 1, wherein: the lower part of the culture solution collecting tank is provided with a waste liquid outlet.
8. A method of microalgae cell harvesting using the harvesting device of any of claims 1-7, characterized in that: the method comprises the following steps:
(1) The microalgae cells and the culture solution are distributed on the bed surface of the filter residue bed, and impurities in the microalgae cells and the culture solution are filtered through a filtering structure of the bed surface of the filter residue bed;
(2) The microalgae cells and the culture solution which are filtered to remove impurities in the step (1) enter the surface of the harvesting bed, so that the culture solution and the microalgae cells are rapidly separated, and the microalgae cells are collected and discharged from an algae mud output port at the bottom of the harvesting bed.
9. A method for harvesting spirulina capable of synthesizing glyceroglycosides using the device of any one of claims 1-7, characterized in that: the method comprises the following steps:
(1) Firstly, spirulina and culture solution are sent into a liquid inlet pipe through an algae solution conveying pump, the spirulina and the culture solution are uniformly distributed on a filter residue bed surface through a liquid distribution hole by the liquid inlet pipe, impurities in the spirulina and the culture solution are filtered through a membrane hole filtering structure on the bed surface, after the impurities are collected in a filter residue collecting tank, the impurities are cleared from a filter residue outlet by a filter residue cleaning rod and enter a filter residue channel, and finally, a harvesting system is cleared, and the spirulina and the culture solution with the impurities filtered out enter a harvesting bed;
(2) The spirulina and the culture solution which are filtered to remove impurities in the step (1) enter the bed surface of the harvesting bed, and the inclination angle alpha of the bed surface relative to the horizontal plane is continuously changed from 0 degree to 90 degrees or is changed in a echelon manner; meanwhile, salt-containing clean water with the same concentration as the culture solution and arranged in the clean water tank enters the cleaning spray pipe after passing through the hollow rotating shaft, and the clean water is sprayed out from the spray holes of the spray cleaning pipe along with the rotation of the rotating shaft to uniformly clean the spirulina on the surface of the harvesting bed, and the jet direction of the spray holes is inclined at an angle of 45-90 degrees with the flowing direction of the spirulina, so that the culture solution and the spirulina are rapidly separated, and the spirulina is discharged from the alga mud output port at the bottom of the harvesting bed after being collected for the next separation and extraction; and the culture solution enters the culture solution collecting tank through the membrane hole filtering structure on the bed surface and is discharged from the waste liquid outlet.
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