CN108721964A - A kind of preparation method of inserted streaming filter membrane - Google Patents
A kind of preparation method of inserted streaming filter membrane Download PDFInfo
- Publication number
- CN108721964A CN108721964A CN201711417092.3A CN201711417092A CN108721964A CN 108721964 A CN108721964 A CN 108721964A CN 201711417092 A CN201711417092 A CN 201711417092A CN 108721964 A CN108721964 A CN 108721964A
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- China
- Prior art keywords
- streaming
- pipes
- preparation
- sample
- diameter
- Prior art date
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- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000012528 membrane Substances 0.000 title claims abstract description 12
- 150000001875 compounds Chemical class 0.000 claims abstract description 3
- 239000000203 mixture Substances 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 12
- 238000001914 filtration Methods 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- BZSXEZOLBIJVQK-UHFFFAOYSA-N 2-methylsulfonylbenzoic acid Chemical compound CS(=O)(=O)C1=CC=CC=C1C(O)=O BZSXEZOLBIJVQK-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000009288 screen filtration Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/085—Funnel filters; Holders therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of preparation method of inserted streaming filter membrane, which is as follows：Round 400 mesh filter screens of diameter 3x3cm are taken to be folded from one piece of 120 degree of angle sizes of center clip, blend compounds connect tapered, a diameter of 0.96cm；By the one circle spill snap ring of circular edge connection of conical screen, to block streaming pipe or EP pipes；Conical screen is put on streaming pipe or EP pipes；Sample addition filter screen is freely dripped, remove filter screen, up flow type machine, filter membrane prepared by this method is time saving, quickly, improves operating efficiency, it loses small, it is suitble to the sample that need to largely handle, more effective for the sample for detecting a small amount of volume, the strainer that can be embedded on streaming pipe or EP pipes can effectively improve the streaming sample process efficiency before machine.
Description
Technical field
The present invention relates to filter membrane preparing technical field, specific field is a kind of preparation method of inserted streaming filter membrane.
Background technology
Flow cytometry (flow cytometry, FCM) is the important skill that cell biology is studied from the mid-1970s
Art, oneself is widely used in the fields such as oncology, immunology, hematology, materia medica so far and the routine work of clinical examination is examined
In survey.The key instrument flow cytometer (FCM) of the technology is integrated monoclonal antibody, fluorescence chemical, laser and computer etc.
A kind of advanced instrument that high technology development gets up.It can quickly be measured by automatically analyzing and sorting to cell, storage,
Show the characteristic parameter in terms of a series of important biophysics of cell dispersion to float on a liquid, biochemistry, and can
Specified cell subsets is therefrom sorted out according to the parameter ranges of pre-selection.FCM can be to the individual cells of big quantity simultaneously
Multiple physics and chemical parameters detection are carried out, including the size of cell, the phase of intracellular granular, cell complexity and cell
To fluorescence intensity etc..Therefore instrument more expensive (price of a streaming instrument hundreds of thousands-is millions of differ) and accurate, needs
Want the meticulous maintenance and use of operating personnel.There is stringent regulation particularly with the requirement of loading sample.It is more most important just
Be carry out filtration treatment before all loading samples will go up machine, in order to avoid cell mass or cell tissue fragment or clogging deposits spray
Mouth or pipeline.Sample blocking can cause：(1) image for influencing DNA makes test result that can not analyze or detect numerical value inaccurate
Really；(2) loading speed is slow；(3) instrument pipeline obstruction can not be continuing with.Although problem above occur can pass through 10% time
Sodium chlorate rinses repeatedly or flooding nozzle, or even dismounting nozzle cleaning down is to solve choking phenomenon.But repeatedly dismounting or
Serious pipeline obstruction can lead to the damage of instrument, be allowed to not work and must not be changed without part or instrument.Therefore, before loading
Filtering be step essential and most important in instrument working service.Usually sample is needed by 400 before upper machine
Purpose filter screen filtration, and dispel resuspension with loading rifle.But there are the following problems in actual use for this step operation：
(1) filter screen is usually the square that 2-3x2-3cm sizes are voluntarily cut by operator, and refolding is at funnel
Shape is placed on stream test tube or EP pipes, is filtered sample.It is time-consuming and laborious if great amount of samples needs processing；
(2) after homemade funnel filter net filtration, sample losses are more；
(3) if placed loosely, strainer, which easily falls into pipe or jumps out, leads to sample losses.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of inserted streaming filter membrane, to solve in above-mentioned background technology
The problem of proposition.
To achieve the above object, the present invention provides the following technical solutions：A kind of preparation method of inserted streaming filter membrane, should
Preparation method is as follows：
Step 1)：Round 400 mesh filter screens of diameter 3x3cm are taken, take 120 degree of angle sizes one piece is cut off from center, is folded, and
It is tapered with glue connection, a diameter of 0.96cm (step A in Fig. 1)；
Step 2)：By the one circle spill snap ring of circular edge connection of conical screen, (Fig. 1 is managed to block streaming pipe or EP
Middle step B)；
Step 3)：Conical screen is put on streaming pipe or EP pipes (step C in Fig. 1)；
Step 4)：Sample addition filter screen is freely dripped, filter screen, up flow type machine are removed.
Preferably, streaming pipe or EP nozzle diameter outer diameters are 1.2cm, and internal diameter is 0.96cm.
Compared with prior art, the beneficial effects of the invention are as follows：A kind of preparation method of inserted streaming filter membrane, it is time saving,
Quickly, operating efficiency is improved, loss is small, is suitble to the sample that need to largely handle, more effective for the sample for detecting a small amount of volume, can
It can effectively improve the streaming sample process efficiency before upper machine with the strainer being embedded on streaming pipe or EP pipes.
Description of the drawings
Fig. 1 is the preparation flow schematic diagram of the present invention.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, the present invention provides a kind of technical solution：A kind of preparation method of inserted streaming filter membrane, the preparation
Method is as follows：
Round 400 mesh filter screens of diameter 3x3cm are taken, take 120 degree of angle sizes one piece is cut off from center, is folded, blend compounds connect
Tapered, a diameter of 0.96cm is met, as step A is demonstrated in figure；
By the one circle spill snap ring of circular edge connection of conical screen, to block streaming pipe or EP pipes such as step B in figure
Demonstration；
Conical screen is put on streaming pipe or EP pipes as step C is demonstrated in figure；
Sample addition filter screen is freely dripped, filter screen, up flow type machine are removed.
Specifically, streaming pipe or EP nozzle diameter outer diameters are 1.2cm, internal diameter is 0.96cm.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (2)
1. a kind of preparation method of inserted streaming filter membrane, it is characterised in that：The preparation method is as follows：
Step 1)：Round 400 mesh filter screens of diameter 3x3cm are taken, take 120 degree of angle sizes one piece is cut off from center, is folded, blend compounds
Connect tapered, a diameter of 0.96cm (in Fig. 1 shown in step A)；
Step 2)：By one circle spill snap ring of the circular edge of conical screen connection, to block streaming pipe or EP pipes, (B is walked in Fig. 1
Shown in rapid)；
Step 3)：Conical screen is put on streaming pipe or EP pipes (in Fig. 1 shown in step C)；
Step 4)：Sample addition filter screen is freely dripped, filter screen, up flow type machine are removed.
2. a kind of preparation method of inserted streaming filter membrane according to claim 1, it is characterised in that：Streaming pipe or EP pipes
Bore outer diameter is 1.2cm, and internal diameter is 0.96cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711417092.3A CN108721964A (en) | 2017-12-15 | 2017-12-15 | A kind of preparation method of inserted streaming filter membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711417092.3A CN108721964A (en) | 2017-12-15 | 2017-12-15 | A kind of preparation method of inserted streaming filter membrane |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108721964A true CN108721964A (en) | 2018-11-02 |
Family
ID=63940937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711417092.3A Pending CN108721964A (en) | 2017-12-15 | 2017-12-15 | A kind of preparation method of inserted streaming filter membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108721964A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11072775B2 (en) * | 2019-07-12 | 2021-07-27 | Biosherpa, Llc | Biological transport systems and methods |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203043677U (en) * | 2012-12-20 | 2013-07-10 | 谭明 | Water impurity measuring cup |
| CN106390600A (en) * | 2016-10-26 | 2017-02-15 | 李兆亿 | Novel filter paper for improving paper utilization rate, and use method and cutting methods thereof |
| CN206411133U (en) * | 2016-11-16 | 2017-08-15 | 苏州大学 | Sample loading tube for flow cytometer |
-
2017
- 2017-12-15 CN CN201711417092.3A patent/CN108721964A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203043677U (en) * | 2012-12-20 | 2013-07-10 | 谭明 | Water impurity measuring cup |
| CN106390600A (en) * | 2016-10-26 | 2017-02-15 | 李兆亿 | Novel filter paper for improving paper utilization rate, and use method and cutting methods thereof |
| CN206411133U (en) * | 2016-11-16 | 2017-08-15 | 苏州大学 | Sample loading tube for flow cytometer |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11072775B2 (en) * | 2019-07-12 | 2021-07-27 | Biosherpa, Llc | Biological transport systems and methods |
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| Date | Code | Title | Description |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: Lin Wei Document name: Notification of Passing Examination on Formalities |
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181102 |