CN108606329A - A kind of prebiotics, probiotics compound micro-ecological preparation and preparation method thereof - Google Patents
A kind of prebiotics, probiotics compound micro-ecological preparation and preparation method thereof Download PDFInfo
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- CN108606329A CN108606329A CN201810412982.3A CN201810412982A CN108606329A CN 108606329 A CN108606329 A CN 108606329A CN 201810412982 A CN201810412982 A CN 201810412982A CN 108606329 A CN108606329 A CN 108606329A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 59
- 239000006041 probiotic Substances 0.000 title claims abstract description 43
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 43
- 235000013406 prebiotics Nutrition 0.000 title claims abstract description 41
- 150000001875 compounds Chemical class 0.000 title claims abstract description 29
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 31
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 31
- 235000015141 kefir Nutrition 0.000 claims abstract description 25
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000001963 growth medium Substances 0.000 claims abstract description 14
- 239000000126 substance Substances 0.000 claims abstract description 12
- 239000004310 lactic acid Substances 0.000 claims abstract description 11
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 11
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 9
- 239000008187 granular material Substances 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 238000000855 fermentation Methods 0.000 claims description 17
- 230000004151 fermentation Effects 0.000 claims description 17
- 150000004676 glycans Chemical class 0.000 claims description 8
- 229920001282 polysaccharide Polymers 0.000 claims description 8
- 239000005017 polysaccharide Substances 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 241001327634 Agaricus blazei Species 0.000 claims description 4
- 240000000588 Hericium erinaceus Species 0.000 claims description 3
- 235000007328 Hericium erinaceus Nutrition 0.000 claims description 3
- 229920001491 Lentinan Polymers 0.000 claims description 3
- 239000000654 additive Substances 0.000 claims description 3
- 230000000996 additive effect Effects 0.000 claims description 3
- 229940115286 lentinan Drugs 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims 1
- 239000003595 mist Substances 0.000 claims 1
- 238000005507 spraying Methods 0.000 claims 1
- 239000000843 powder Substances 0.000 abstract description 17
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract description 12
- 235000020183 skimmed milk Nutrition 0.000 abstract description 9
- 238000001694 spray drying Methods 0.000 abstract description 8
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 abstract description 6
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 abstract description 6
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 abstract description 6
- 235000011187 glycerol Nutrition 0.000 abstract description 6
- 230000036039 immunity Effects 0.000 abstract description 5
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 abstract description 4
- 235000013923 monosodium glutamate Nutrition 0.000 abstract description 4
- 229940073490 sodium glutamate Drugs 0.000 abstract description 4
- 239000003223 protective agent Substances 0.000 abstract description 3
- 230000036541 health Effects 0.000 abstract description 2
- 238000002156 mixing Methods 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 description 13
- 239000002609 medium Substances 0.000 description 13
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 10
- 108010046377 Whey Proteins Proteins 0.000 description 10
- 102000007544 Whey Proteins Human genes 0.000 description 10
- 230000001954 sterilising effect Effects 0.000 description 9
- 238000004659 sterilization and disinfection Methods 0.000 description 9
- 239000012530 fluid Substances 0.000 description 8
- 239000002054 inoculum Substances 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 8
- 229910052799 carbon Inorganic materials 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 239000005862 Whey Substances 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 5
- 235000019341 magnesium sulphate Nutrition 0.000 description 5
- 229940099596 manganese sulfate Drugs 0.000 description 5
- 239000011702 manganese sulphate Substances 0.000 description 5
- 235000007079 manganese sulphate Nutrition 0.000 description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 5
- 235000013336 milk Nutrition 0.000 description 5
- 239000008267 milk Substances 0.000 description 5
- 210000004080 milk Anatomy 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 235000021119 whey protein Nutrition 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 238000013019 agitation Methods 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 238000005238 degreasing Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 210000001035 gastrointestinal tract Anatomy 0.000 description 4
- 238000000386 microscopy Methods 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Natural products CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- SNGZVXPRGLTKNU-RGMNGODLSA-N [Na].C(CC)N[C@@H](CCO)C(=O)O Chemical compound [Na].C(CC)N[C@@H](CCO)C(=O)O SNGZVXPRGLTKNU-RGMNGODLSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000007366 host health Effects 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- YNPNZTXNASCQKK-UHFFFAOYSA-N phenanthrene Chemical compound C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000289669 Erinaceus europaeus Species 0.000 description 1
- 241000577951 Hydnum Species 0.000 description 1
- 229920001202 Inulin Polymers 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 235000020167 acidified milk Nutrition 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000019993 champagne Nutrition 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000012263 liquid product Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 238000002663 nebulization Methods 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of prebiotics, probiotics compound micro-ecological preparation and preparation method thereof, and the application's mainly comprises the following steps：By choosing lactic acid bacteria, saccharomycete and the strain that bacillus subtilis is compound micro-ecological preparation, wherein lactic acid bacteria and saccharomycete come from Kefir granule, Kefir granule and bacillus subtilis are activated respectively, by Kefir granule and bacillus subtilis according to 3:1 ratio mixes; probiotics mixed bacteria is inoculated in culture medium by the 3~10% of fermentating liquid volume, fermented and cultured, adds prebiotics; skimmed milk powder, trehalose, sodium glutamate, glycerine are added simultaneously as protective agent, carries out low temperature spray drying after mixing and probiotics is made.It is characteristic of the invention that containing lactic acid bacteria in probiotics, saccharomycete and bacillus subtilis can adjust body microecological balance, while also contain prebiotic substance jointly, can preferably improve body health, improve immunity of organisms.
Description
Technical field
The invention belongs to technical field of microbial fermentation, be related to a kind of prebiotics, probiotics compound micro-ecological preparation and its
Preparation method.
Background technology
Nineteen forties add antibiotic to promote fast growth of animal in feed, but with antibiosis
Plain long-term extensive use, will produce drug resistance, medicament residue, the drawbacks such as animal body immunity degradation.Probiotics with
The advantages that its green, safety, drug residue free, has gradually replaced antibiotic and has been widely used.With traditional feed addictive phase
Than probiotics micro-ecological formulation can effectively inhibit the growth and breeding of the pathogenic bacteria and pathogenic microorganism in alimentary canal, and make benefit
Raw bacterium is colonized in enteron aisle, and adjustment animal intestinal tract balance has the effect of the prevention of Animal diseases certain.Currently, the world is each
State gradually increases and widelys popularize to the attention degree of probiotics.Prebiotics, probiotics compound micro-ecological preparation are with micro-
Ecology is used as theoretical foundation, is made by special processing process, micro- in animal body to improve containing viable bacteria and its metabolite
The ecological balance and play prebiotic effect, improve animal immunizing power and the general level of the health active bacteria formulation.
A kind of replenishers of diet of prebiotics pass through the growth and work of the one or more of probiotics of the stimulation of selectivity
Property, so as to improve substance of the host health without being digested absorption.Common prebiotics have oligofructose, inulin, hedgehog hydnum
Mushroom polysaccharide, Agaricus Blazei Murrill polysaccharide and lentinan etc..Prebiotics cannot be utilized directly by body and be played a role, but pass through probiotics
It plays a role jointly.Do not have to decompose the substance of prebiotics in body alimentary canal, therefore its most of prebiotic substance can pass through machine
Body small intestine and stomach are utilized without being decomposed, and are directly entered big enteral and are utilized by probiotics such as Bifidobacterium, lactic acid bacterias.
Kefir fermenting agent be by lactic acid bacteria, saccharomycete, a variety of probiotic groups such as acetic acid bacteria at bacterial strain, lactic acid therein
Bacterium and its metabolite there is strong inhibiting effect, saccharomycete therein can generate unique aroma pathogenic microbes
Taste, thus Kefir grains are also known as champagne in acidified milk.It is a kind of leavening containing more bacterial strains.
The main probiotics preparation of domestic and international market is bacillus subtilis formulation and lactobacillus preparation at present, and majority is more
Kind single culture is prepared, and strain constitutes relatively simple.The present invention is using Kefir fermenting agent as lactic acid bacteria and saccharomycete
Source is mixed with bacillus subtilis, and cultivation and fermentation, addition hericium erinaceum polysaccharide, Agaricus Blazei Murrill polysaccharide and mushroom are mixed through high density
One or more of polysaccharide passes through low temperature spray drying technology, and prebiotics, probiotics compound micro-ecological preparation is made.Micro- life
State additive has total viable count high, can adjust the gastrointestinal tract balance of body, promote body growth, enhancing immunity of organisms etc.
Effect.
Invention content
The purpose of the present invention is to provide a kind of prebiotics, probiotics compound micro-ecological preparation and preparation method thereof, prepare
Prebiotics, probiotics compound micro-ecological preparation not only contain the prebiotic substance for improving host health, while there are many also containing
Probiotics and total viable count height, the effect of playing and adjust body gastrointestinal tract balance, enhance immunity of organisms.
The present invention is realized by following technological means：A kind of prebiotics, probiotics compound micro-ecological preparation, it is compound
Probiotics are formed by prebiotics and probiotic combinations, and the probiotics is by lactic acid bacteria, saccharomycete and bacillus subtilis
Mixed fermentation forms, and lactic acid bacteria and saccharomycete are provided by Kefir granule compound strain, and total viable count is more than 5,000,000,000 in preparation
Cfu/g, the prebiotics are one or more of hericium erinaceum polysaccharide, Agaricus Blazei Murrill polysaccharide and lentinan composition, additive amount
It is 0.3%~1.5%.
The preparation method of prebiotics, probiotics compound micro-ecological preparation：Compound micro-ecological preparation is mixed using a variety of strains
It ferments, after High Density Cultivation, adds prebiotic substance, be made by low temperature spray drying.By probiotics mixed bacteria by fermentation
The 3~10% of liquid product are inoculated in culture medium, and the wherein inoculative proportion of Kefir granule and bacillus subtilis is 3:1,28~36
Under the conditions of DEG C, ferment 16~24 hours.
In above-mentioned preparation, the culture medium contains following components by weight percentage：Concentrated whey protein powder 2~
10%, glucose 1~10%, yeast powder 0.5~1%, calcium carbonate 0.4~1%, magnesium sulfate 0.01~0.05%, manganese sulfate
0.001~0.005%, whey powder 1~10%.Mentioned component is dissolved in distilled water, and it is 6.5~7.5 to adjust medium pH,
Sterilization conditions keep the temperature 10~30min under the conditions of being 85~115 DEG C, are cooled to 20~30 DEG C.
In above-mentioned preparation, the mixed fermentation condition is：Probiotics mixed bacteria is pressed the 3~10% of fermentating liquid volume
It is inoculated in culture medium, the wherein inoculative proportion of Kefir fermenting agent and bacillus subtilis is 3:Under the conditions of 1,28~36 DEG C, hair
Ferment 16~24 hours, the prebiotics and protective agent for adding 0.3%-1.5% are made by spray drying.
The prebiotics, the specific preparation method of probiotics compound micro-ecological preparation include the following steps：
1) bacillus subtilis is activated, and prepares Kefir fermenting agent.
2) probiotics mixed bacteria is inoculated in by the 3~10% of fermentating liquid volume in high-density culture medium, wherein opening phenanthrene
That leavening and the inoculative proportion of bacillus subtilis are：Kefir fermenting agent: bacillus subtilis=3:1, it is inoculated in culture
In base, under the conditions of 28~36 DEG C, ferment 16~24 hours.
3) after fermentation, stirring centrifugation centrifuges 15~30min under the conditions of 3000~6000r/min, collects concentration
Thalline.
4) prebiotics of 0.3%-1.5% are added into concentration thalline, while skimmed milk powder 10~20%, trehalose is added
1~10%, sodium glutamate 1~10%, glycerine 1~5% are used as protective agent, carry out low temperature spray drying after mixing, control
Inlet air temperature is 80~120 DEG C, 50~70 DEG C of leaving air temp, to ensure to contain a certain amount of total viable count in the preparation prepared.
The beneficial effects of the present invention are：
1) Kefir fermenting agent and bacillus subtilis are carried out mixed culture fermentation, the probiotics of preparation by the present invention
Strain is various, and total viable count is up to 5,000,000,000 cfu/g or more；2) the composite bacteria of the invention addition pair after mixed culture is fermented
The beneficial prebiotic substance of body adjusts body gastrointestinal tract balance to preferably play, promotes body digestion, increase body body
Weight, enhancing immunity of organisms and other effects.
Specific implementation mode
1) preparation of probiotics leaven：
The preparation of Kefir fermenting agent：The degreasing milk solution of configuration 10%, by 85~95 DEG C, after 10~30min sterilizations,
20~30 DEG C are cooled to, Kefir grains throw type leaven is added according to the 0.1~0.5% of skimmed milk volume, at 23~28 DEG C
Under the conditions of 14~18h of fermented and cultured, it is spare under the conditions of being stored in 4 DEG C.
The preparation of bacillus subtilis：The bacillus subtilis frozen is subjected to scribing line culture, colonies typical is isolated and connects
Kind shake culture 18h under the conditions of 37 DEG C in fluid nutrient medium, the standard type of bacillus subtilis is met through microscopy, grows shape
State is good, and being inoculated in fluid nutrient medium by inoculum concentration 3~6% is activated, 37 DEG C, and shake culture 18~for 24 hours, it is stored in 4 DEG C
Under the conditions of it is spare.
2) mixed fermentation：
The configuration of culture medium：10~20g of whey protein concentrate, 6~8g of glucose, yeast are added into 500mL distilled water
3~5g of powder, 3~6g of calcium carbonate, 0.1~0.3g of magnesium sulfate, 0.01~0.03g of manganese sulfate, 10~15g of whey powder fully dissolve
It is 6.5~7.5 to adjust medium pH afterwards, and sterilization conditions keep the temperature 10~30min under the conditions of being 85~115 DEG C, are cooled to 20~30
It is DEG C spare.To culture medium inoculated after cooling, by 3~6% inoculum concentrations, wherein Kefir fermenting agent：Bacillus subtilis=3：
1,28~36 DEG C, fermented and cultured 18~for 24 hours.
3) preparation of compound micro-ecological preparation：
Fermentation broth agitation obtained is uniform, under the conditions of 4~10 DEG C, 3000~6000 revs/min of rotating speed, the time 15~
30min centrifugal treatings collect concentration thalline；Based on thalline mass percent, the prebiotic substance of 0.3%-1.5% is added, is taken off
Fat milk powder 10~15%, trehalose 1~5%, sodium glutamate 1~5%, glycerine 1~3% are uniformly mixed, and it is dry to carry out cold nebulization
Dry, control inlet air temperature is 80~120 DEG C, and 50~70 DEG C of leaving air temp obtains prebiotics, probiotics compound micro-ecological preparation.
The following examples are further illustrations of the invention, rather than limiting the invention.
Embodiment 1：
1) preparation of probiotics leaven
The preparation of Kefir fermenting agent：The degreasing milk solution of configuration 10% after 30min sterilizations, is cooled to 20 by 85 DEG C
DEG C, Kefir grains throw type leaven is added according to the 0.1% of skimmed milk volume, fermented and cultured 14h under conditions of 28 DEG C is preserved
It is spare under the conditions of 4 DEG C.
The preparation of bacillus subtilis：The bacillus subtilis frozen is subjected to scribing line culture, colonies typical is isolated and connects
Kind shake culture 18h under the conditions of 37 DEG C in fluid nutrient medium, the standard type of bacillus subtilis is met through microscopy, grows shape
State is good, and being inoculated in fluid nutrient medium by inoculum concentration 6% is activated, 37 DEG C, shake culture 18h, standby under the conditions of being stored in 4 DEG C
With.
2) mixed fermentation
The configuration of culture medium：Whey protein concentrate 10g, glucose 8g, yeast powder 5g, carbon are added into 500mL distilled water
Sour calcium 3g, magnesium sulfate 0.3g, manganese sulfate 0.01g, whey powder 15g, it is 6.5 that medium pH is fully adjusted after dissolving, sterilization conditions
Keep the temperature 30min under the conditions of being 85 DEG C, be cooled to 20 DEG C it is spare.To culture medium inoculated after cooling, by 3% inoculum concentration, wherein open
Fei Er leavenings：Bacillus subtilis=3：1,28 DEG C, fermented and cultured is for 24 hours.
3) preparation of compound micro-ecological preparation
Fermentation broth agitation obtained is uniform, and under the conditions of 4 DEG C, 3000 revs/min of rotating speed, time 30min centrifugal treating is received
Collection concentration thalline；Based on thalline mass percent, 0.3% prebiotic substance, skimmed milk powder 15%, trehalose 1%, paddy are added
Propylhomoserin sodium 5%, glycerine 1% are uniformly mixed, and carry out low temperature spray drying, and control inlet air temperature is 80 DEG C, 50 DEG C of leaving air temp,
Obtain prebiotics, probiotics compound micro-ecological preparation.
Embodiment 2
1) preparation of probiotics leaven
The preparation of Kefir fermenting agent：The degreasing milk solution of configuration 10% after 20min sterilizations, is cooled to 25 by 90 DEG C
DEG C, Kefir grains throw type leaven is added according to the 0.3% of skimmed milk volume, fermented and cultured 18h under conditions of 23 DEG C is preserved
It is spare under the conditions of 4 DEG C.
The preparation of bacillus subtilis：The bacillus subtilis frozen is subjected to scribing line culture, colonies typical is isolated and connects
Kind shake culture 18h under the conditions of 37 DEG C in fluid nutrient medium, the standard type of bacillus subtilis is met through microscopy, grows shape
State is good, and being inoculated in fluid nutrient medium by inoculum concentration 3% is activated, 37 DEG C, and shake culture is for 24 hours, standby under the conditions of being stored in 4 DEG C
With.
2) mixed fermentation
The configuration of culture medium：Whey protein concentrate 20g, glucose 6g, yeast powder 3g, carbon are added into 500mL distilled water
Sour calcium 6g, magnesium sulfate 0.2g, manganese sulfate 0.02g, whey powder 10g, it is 7.5 that medium pH is fully adjusted after dissolving, sterilization conditions
Keep the temperature 10min under the conditions of being 115 DEG C, be cooled to 30 DEG C it is spare.To culture medium inoculated after cooling, by 6% inoculum concentration, wherein
Kefir fermenting agent：Bacillus subtilis=3：1,36 DEG C, fermented and cultured 18h.
3) preparation of compound micro-ecological preparation
Fermentation broth agitation obtained is uniform, and under the conditions of 8 DEG C, 4500 revs/min of rotating speed, time 25min centrifugal treating is received
Collection concentration thalline；Based on thalline mass percent, 0.9% prebiotic substance, skimmed milk powder 12%, trehalose 3%, paddy are added
Propylhomoserin sodium 1%, glycerine 2% are uniformly mixed, and carry out low temperature spray drying, and control inlet air temperature is 120 DEG C, 70 DEG C of leaving air temp,
Obtain prebiotics, probiotics compound micro-ecological preparation.
Embodiment 3
1) preparation of probiotics leaven
The preparation of Kefir fermenting agent:The degreasing milk solution of configuration 10% after 10min sterilizations, is cooled to 30 by 95 DEG C
DEG C, Kefir grains throw type leaven is added according to the 0.5% of skimmed milk volume, fermented and cultured 16h under conditions of 25 DEG C is preserved
It is spare under the conditions of 4 DEG C.
The preparation of bacillus subtilis:The bacillus subtilis frozen is subjected to scribing line culture, colonies typical is isolated and connects
Kind shake culture 18h under the conditions of 37 DEG C in fluid nutrient medium, the standard type of bacillus subtilis is met through microscopy, grows shape
State is good, and being inoculated in fluid nutrient medium by inoculum concentration 5% is activated, 37 DEG C, shake culture 20h, standby under the conditions of being stored in 4 DEG C
With.
2) mixed fermentation
The configuration of culture medium：Whey protein concentrate 15g, glucose 7g, yeast powder 4g, carbon are added into 500mL distilled water
Sour calcium 4g, magnesium sulfate 0.1g, manganese sulfate 0.03g, whey powder 12g, it is 7.0 that medium pH is fully adjusted after dissolving, sterilization conditions
Keep the temperature 20min under the conditions of being 100 DEG C, be cooled to 25 DEG C it is spare.To culture medium inoculated after cooling, by 4% inoculum concentration, wherein
Kefir fermenting agent：Bacillus subtilis=3：1,32 DEG C, fermented and cultured 20h.
3) preparation of compound micro-ecological preparation
Fermentation broth agitation obtained is uniform, under the conditions of 10 DEG C, 6000 revs/min of rotating speed, time 15min centrifugal treating,
Collect concentration thalline；Based on thalline mass percent, add 1.5% prebiotic substance, skimmed milk powder 10%, trehalose 5%,
Sodium glutamate 3%, glycerine 3% are uniformly mixed, and carry out low temperature spray drying, and control inlet air temperature is 100 DEG C, leaving air temp 60
DEG C, obtain prebiotics, probiotics compound micro-ecological preparation.
Claims (3)
1. a kind of prebiotics, probiotics compound micro-ecological preparation, it is characterised in that：Compound micro-ecological preparation is by prebiotics and prebiotic
Bacterium is composed, and the probiotics is formed by lactic acid bacteria, saccharomycete and bacillus subtilis mixed fermentation, lactic acid bacteria and yeast
Bacterium is provided by Kefir granule compound strain, and total viable count is more than 5,000,000,000 cfu/g in preparation, and the prebiotics are that Hericium erinaceus is more
One or more of sugar, Agaricus Blazei Murrill polysaccharide and lentinan composition, additive amount are 0.3%~1.5%.
2. the preparation method of prebiotics according to claim 1, probiotics compound micro-ecological preparation, which is characterized in that system
Standby compound micro-ecological preparation uses a variety of strain mixed fermentations, after High Density Cultivation, prebiotic substance is added, by low-temp. spraying
Mist drying is made.
3. the preparation method of prebiotics according to claim 2, probiotics compound micro-ecological preparation, it is characterised in that：It will
Probiotics mixed bacteria is inoculated in culture medium by the 3~10% of fermentating liquid volume, wherein Kefir granule and bacillus subtilis
Inoculative proportion is 3:Under the conditions of 1,28~36 DEG C, ferment 16~24 hours.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112457990A (en) * | 2020-12-18 | 2021-03-09 | 中国科学院合肥物质科学研究院 | Freeze-drying protective agent and application thereof |
| CN113181220A (en) * | 2021-05-18 | 2021-07-30 | 郭云 | Bacillus subtilis spray and preparation method thereof |
| CN113789276A (en) * | 2021-09-15 | 2021-12-14 | 千宠堂(福建)生物科技有限公司 | Lactobacillus plantarum for producing beta-nicotinamide mononucleotide and application thereof |
| CN113841901A (en) * | 2021-09-15 | 2021-12-28 | 广西大学 | Preparation method of high-activity synbiotics preparation freeze-dried powder |
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2018
- 2018-05-03 CN CN201810412982.3A patent/CN108606329A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112457990A (en) * | 2020-12-18 | 2021-03-09 | 中国科学院合肥物质科学研究院 | Freeze-drying protective agent and application thereof |
| CN113181220A (en) * | 2021-05-18 | 2021-07-30 | 郭云 | Bacillus subtilis spray and preparation method thereof |
| CN113789276A (en) * | 2021-09-15 | 2021-12-14 | 千宠堂(福建)生物科技有限公司 | Lactobacillus plantarum for producing beta-nicotinamide mononucleotide and application thereof |
| CN113841901A (en) * | 2021-09-15 | 2021-12-28 | 广西大学 | Preparation method of high-activity synbiotics preparation freeze-dried powder |
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