CN108319820A - A kind of method of plant heavy carbonate equipment usage under acquisition outdoor habitatss - Google Patents
A kind of method of plant heavy carbonate equipment usage under acquisition outdoor habitatss Download PDFInfo
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- CN108319820A CN108319820A CN201810313290.3A CN201810313290A CN108319820A CN 108319820 A CN108319820 A CN 108319820A CN 201810313290 A CN201810313290 A CN 201810313290A CN 108319820 A CN108319820 A CN 108319820A
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Abstract
The invention discloses a kind of methods of plant heavy carbonate equipment usage under acquisition outdoor habitatss, belong to crop information detection technique and ecological environment treatment field, extracellular atmospheric carbon dioxide concentration and intercellular gas concentration lwevel data are obtained using portable photosynthetic instrument, the plant of different seedling age different growings under varying environment is measured simultaneously using the stable carbon isotope fractionation value of the carbon dioxide of air and the heavy carbonate of soil, in conjunction with atmospheric carbon dioxide stable carbon isotope composition, the stable carbon isotope composition of the rhizosphere soil heavy carbonate of plant；The plant that different seedling age different growings under varying environment are obtained according to both-end member isotopic dating method utilizes the instant messages of heavy carbonate equipment usage.The plant that quick, convenient, accurate, real-time can not only obtain different seedling age different growings under outdoor habitatss utilizes atmospheric carbon dioxide equipment usage, while can also obtain plant heavy carbonate equipment usage under outdoor habitatss.
Description
Technical field
The present invention relates to crop information detection technique and ecological environment treatment fields, more particularly to a kind of acquisition open country
The method of plant heavy carbonate equipment usage, may not need and cultivate plant cumbersomely under outer habitat, so that it may the real time measure difference seedling
The plant of age different growing utilizes the information of heavy carbonate, the screening for karst adaptive plant to provide scientific basis.
Background technology
In recent years, numerous experiments is it has been proved that plant can not only be carried out using the carbon dioxide in air as substrate
Photosynthesis, and photosynthesis can also be carried out using the heavy carbonate for coming from soil.Especially in the weight with high concentration
The karst In Limestone Area of carbonate only measures the inorganic of plant with based on the photosynthetic instrument for measuring atmospheric carbon dioxide flux
Carbon assimilation ability seriously underestimates the productivity of karst plant.
Different plants under identical conditions have different heavy carbonate equipment usages, same plant under various circumstances,
Heavy carbonate equipment usage also differs greatly.Although there is certain methods that can measure the laboratory cultures in whether field
Plant heavy carbonate Utilization ability, still, these methods are all difficult under the real time measure varying environment different seedling age differences lifes
The plant for educating the phase utilizes the information of heavy carbonate, therefore, the plant of different seedling age different growings under Accurate Determining varying environment
Using heavy carbonate equipment usage, to correctly assessing the productivity of plant, the karst adaptive plant kind of high productivity is screened,
It is administered with karst adaptive plant and restores fragile Karst ecosystem and played an important role.
Invention content
The technical problem to be solved by the present invention is to provide plant heavy carbonate equipment usage under a kind of acquisition outdoor habitatss
Method, not only be not necessarily to cultivate cumbersomely plant can the real time measure difference seedling age different growing plant heavy carbonate utilize part
Volume, and also overcome the defect that the prior art cannot obtain plant heavy carbonate using information in batches.
The present invention takes following technical scheme：It includes the following steps：
Step 1, the blade of second expansion leaf of selection in the environment of being investigated on growing plants shoot, sunny
Under weather, the morning 9 is measured respectively using portable photosynthetic instrument：00-10：00 photosynthetic parameters；Second on so-called plant shoot
The blade of expansion leaf is according to principle from top to bottom, is first complete just to have developed the leaf completely and being fully deployed on shoot
Leaf is unfolded, and so on；Respectively the second fully expanded leaves, third fully expanded leaves；
Step 2 obtains extracellular atmospheric carbon dioxide concentration Ca and intercellular gas concentration lwevel Ci from portable photosynthetic instrument
Data, calculate plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca ratio f, computational methods
For：
Step 3, the blade of first expansion leaf of selection in the environment of being investigated on growing plants shoot, using normal
The stable carbon isotope that rule method measures plant leaf blade forms δ13The value of C, is denoted as δT；
Step 4 obtains form δ by investigation ambient air carbon dioxide stable carbon isotope according to a conventional method13C's is averaged
Value, is denoted as δa；
Step 5 measures the steady of the rhizosphere soil heavy carbonate of growing plants in the environment of being investigated according to a conventional method
Determine carbon isotope composition δ13The value of C, is denoted as δb；
Step 6, the ratio f according to plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca；
Calculate the stable carbon isotope fractionation value Δ a of growing plants blade assimilation carbon dioxide in the environment of being investigated；Calculating side
Method is：Δ a=4.4 ‰+(D-4.4 ‰) f, wherein fractionation when D is Rubsico carboxylations to inorganic carbon, according to plant
Different values are different, and ranging from 27 ‰~29 ‰；
Step 7, the stable carbon isotope point according to growing plants blade assimilation carbon dioxide in the environment of being investigated
Value Δ a is evaporated, the stable carbon isotope fractionation value Δ of growing plants blade assimilation heavy carbonate in the environment of being investigated is calculated
b；Computational methods are：Δ b=Δs a-4.4 ‰ -9.9 ‰；
Step 8, the stable carbon isotope according to growing plants blade in the environment of being investigated form δ13The value δ of CT、
δ is formed by investigation ambient air carbon dioxide stable carbon isotope13The average value δ of Ca, the plant that grows in the environment of being investigated
The stable carbon isotope of the rhizosphere soil heavy carbonate of object forms δ13The value δ of Cb；Growing plants leaf in the environment of being investigated
Piece assimilates the stable carbon isotope fractionation value Δ a of carbon dioxide, growing plants blade assimilation weight carbon in the environment of being investigated
The stable carbon isotope fractionation value Δ b of hydrochlorate calculates plant heavy carbonate equipment usage fbComputational methods be：
The present invention basic principle be：
The strong fractionation of stable carbon isotope is characterized in identifying the basis of different inorganic carbon sources in plant.Carbon in nature
There are two types of stable isotopes for element：12C and13C, their natural average abundance is respectively 98.89% and 1.11%.Stable Carbon is same
Position element composition usually uses δ13C (‰) indicates, δ in nature13The variation of C is -90 ‰~+20 ‰.Stable carbon isotope it is strong
Fractionation feature is conducive to identify different inorganic carbon sources in plant.Mass balance principle and isotopic dating method and chemistry
Metrology method is the basis of different inorganic carbon sources in quantitative judge plant.
Both-end member isotopic dating method can be expressed as：
δT=δA-fbδA+fbδB (1)
Here δTFor the δ of investigated plant blade13C values, δATo assume that plant using atmospheric carbon dioxide is sole carbon source
When blade δ13C values, δBTo assume that the δ of blade when the heavy carbonate from root is sole carbon source is fully utilized in plant13C values,
fbFor the investigation plant share that plant utilizes total inorganic carbon source is accounted for using the bicarbonate ion that external source is added.
Plant is exercising C3When approach, blade meets following relationship (2) to the fractionation Δ a of air carbon dioxide.
Δ a=a+ (D-a) (Ci/Ca) (2)
In formula (2), Ci is intercellular gas concentration lwevel, and Ca is the gas concentration lwevel in air, and a is stomata diffusion
When fractionation to inorganic carbon, value 4.4 ‰, fractionation when D is Rubsico carboxylations to inorganic carbon, the difference according to plant
Value is different, and ranging from 27 ‰~29 ‰；It enablesAbove known parameters substitution (2) formula is obtained：
Δ a=4.4 ‰+(D-4.4 ‰) f (3)
From (3), formula can be seen that C3To the fractionation value of inorganic carbon and intercellular two when carbon dioxide in plant assimilating air
It is directly proportional to aoxidize concentration of carbon.(3) formula is deformed into (4) formula, is obtained：
δA=δa-Δa (4)
In formula (4), δAFor δAThe δ of blade when to assume that plant using atmospheric carbon dioxide is sole carbon source13C values, δaFor sky
Carbon dioxide stable carbon isotope ratio in gas.
When carbon dioxide hydrolyses are at bicarbonate ion inorganic carbon fractionation be -9.9 ‰, the bicarbonate radical that plant utilizes from
Son comes from root, and existing isotope diffusional fractionation when without as carbon dioxide gas into blowhole, therefore, blade is to coming from
The fractionation Δ b of the inorganic carbon of the heavy carbonate of root meets following relationship (5).
Δ b=Δs a-4.4 ‰ -9.9 ‰ (5)
The stable carbon isotope fractionation value Δ b of plant leaf blade assimilation heavy carbonate can convert to come as plant full utilization
The δ of blade when from the heavy carbonate of root being sole carbon source13C values δBWith the same position of Stable Carbon of the rhizosphere soil heavy carbonate of plant
Element composition δ13The value δ of CbDifference, therefore (5) formula can become (6) formula：
ΔB=δb-Δb (6)
(4) formula and (6) formula are updated to (1) formula, can be obtained：
It obtains intercellular gas concentration lwevel Ci, the gas concentration lwevel Ca in air and stable carbon isotope ratio delta a, plant
The stable carbon isotope of object blade forms δ13The value δ of CT, plant rhizosphere soil heavy carbonate stable carbon isotope form δ13C
Value δbPlant heavy carbonate equipment usage f can be calculatedb。
Advantages of the present invention is as follows：
The present invention is then to utilize the two of air by the plant of different seedling age different growings under the real time measure varying environment
The stable carbon isotope fractionation value of the heavy carbonate of carbonoxide and soil, formed in conjunction with atmospheric carbon dioxide stable carbon isotope,
The stable carbon isotope of the rhizosphere soil heavy carbonate of plant forms；Varying environment is obtained according to both-end member isotopic dating method
The plant of lower difference seedling age different growing utilizes the instant messages of heavy carbonate equipment usage.
1) present invention can not only obtain plant under outdoor habitatss and is fractionated using the stable carbon isotope of atmospheric carbon dioxide
Value, and the stable carbon isotope fractionation value that plant under outdoor habitatss utilizes the heavy carbonate from soil can also be obtained.
2) present invention can not only obtain the equipment usage of plant under outdoor habitatss to atmospheric carbon dioxide, while can also obtain
Take under outdoor habitatss plant to the equipment usage of heavy carbonate.
3) present invention can quick, convenient, accurate, the different seedling age different growings of the real time measure plant heavy carbonate utilization
Share.
4) present invention not only eliminates the process for cultivating plant cumbersomely, but also can obtain plant heavy carbonate in batches
Utilize information
5) present invention can also provide foundation for the quick screening of karst adaptive plant, be that the environmental suitability of plant is studied
Scientific method is provided.
Specific implementation mode
The embodiment of the present invention：It includes the following steps,
Step 1, the blade of second expansion leaf of selection in the environment of being investigated on growing plants shoot, sunny
Under weather, is used using portable photosynthetic instrument and measure the morning 9 respectively：00-10：00 photosynthetic parameters；On so-called plant shoot
The blade of two expansion leaves is according to principle from top to bottom, is first complete just to have developed the leaf completely and being fully deployed on shoot
Full expansion leaf, and so on；Respectively the second fully expanded leaves, third fully expanded leaves；
Step 2 obtains extracellular atmospheric carbon dioxide concentration Ca and intercellular gas concentration lwevel Ci from portable photosynthetic instrument
Data, calculate plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca ratio f, computational methods
For：
Step 3, the blade of first expansion leaf of selection in the environment of being investigated on growing plants shoot, using normal
The stable carbon isotope that rule method measures plant leaf blade forms δ13The value of C, is denoted as δT；
Step 4 obtains form δ by investigation ambient air carbon dioxide stable carbon isotope according to a conventional method13C's is averaged
Value, is denoted as δa；
Step 5 measures the steady of the rhizosphere soil heavy carbonate of growing plants in the environment of being investigated according to a conventional method
Determine carbon isotope composition δ13The value of C, is denoted as δb；
Step 6, the ratio f according to plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca；
Calculate the stable carbon isotope fractionation value Δ a of growing plants blade assimilation carbon dioxide in the environment of being investigated；Calculating side
Method is：Δ a=4.4 ‰+(D-4.4 ‰) f, wherein fractionation when D is Rubsico carboxylations to inorganic carbon, according to plant
Different values are different, and ranging from 27 ‰~29 ‰；
Step 7, the stable carbon isotope point according to growing plants blade assimilation carbon dioxide in the environment of being investigated
Value Δ a is evaporated, the stable carbon isotope fractionation value Δ of growing plants blade assimilation heavy carbonate in the environment of being investigated is calculated
b；Computational methods are：Δ b=Δs a-4.4 ‰ -9.9 ‰；
Step 8, the stable carbon isotope according to growing plants blade in the environment of being investigated form δ13The value δ of CT、
δ is formed by investigation ambient air carbon dioxide stable carbon isotope13The average value δ of Ca, the plant that grows in the environment of being investigated
The stable carbon isotope of the rhizosphere soil heavy carbonate of object forms δ13The value δ of Cb；Growing plants leaf in the environment of being investigated
Piece assimilates the stable carbon isotope fractionation value Δ a of carbon dioxide, growing plants blade assimilation weight carbon in the environment of being investigated
The stable carbon isotope fractionation value Δ b of hydrochlorate calculates plant heavy carbonate equipment usage fbComputational methods be：
Detailed implementation process and content are as follows：
1) determination of three region camplotheca acuminata heavy carbonate equipment usages of Guiyang general mountain July：
Three region camplotheca acuminatas of July on Guiyang general mountain will be grown in as research object, under fair weather, profit
It is used with portable photosynthetic instrument and measures the morning 9 respectively：00-10：The photosynthetic ginseng of the blade of the second expansion leaf on 00 plant shoot
Number；The data of extracellular atmospheric carbon dioxide concentration Ca and intercellular gas concentration lwevel Ci are read from portable photosynthetic instrument, are calculated
The ratio f of plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca, such as table 1；Measure plant simultaneously
The stable carbon isotope of the blade of the first expansion leaf on shoot forms δ13The value δ of CT, such as table 1；It obtains simultaneously by investigation environment
Atmospheric carbon dioxide stable carbon isotope forms δ13The average value δ of CaAnd the Stable Carbon of the rhizosphere soil heavy carbonate of plant is same
Position element composition δ13The value δ of Cb, such as table 1；According to plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration
The ratio f of Ca；The stable carbon isotope fractionation value Δ a for calculating plant leaf blade assimilation carbon dioxide, such as table 2；Computational methods are：Δ
A=4.4 ‰+(D-4.4 ‰) f, wherein the value of D is 27 ‰；Then, the stabilization of camplotheca acuminata blade assimilation heavy carbonic hydrochlorate is calculated
Carbon isotope fractionation value Δ b, such as table 2；Finally, δ is formed according to the stable carbon isotope of camplotheca acuminata blade13The value δ of CT, air dioxy
Change carbon stable carbon isotope and forms δ13The average value δ of Ca, rhizosphere soil heavy carbonate stable carbon isotope form δ13The value of C
δb, camplotheca acuminata blade assimilation carbon dioxide stable carbon isotope fractionation value Δ a, camplotheca acuminata blade assimilation heavy carbonate Stable Carbon it is same
The plain fractionation value Δ b in position, calculates plant heavy carbonate equipment usage fb, such as table 2.
The blade intercellular gas concentration lwevel Ci of table 1 Guiyang general mountain July, three region camplotheca acuminatas and extracellular air two
The stable carbon isotope of the blade of the first expansion leaf on the ratio f of carbonoxide concentration C a, shoot forms δ13The value δ of CT, air
Carbon dioxide stable carbon isotope forms δ13The average value δ of CaAnd the stable carbon isotope of the rhizosphere soil heavy carbonate of plant
Form δ13The value δ of Cb
| Sample | Plant number | f | δa‰ | δT‰ | δb‰ |
| One area | XS-1 | 0.61 | -11.98 | -28.21 | -9.19 |
| One area | XS-2 | 0.66 | -11.98 | -29.44 | -10.00 |
| One area | XS-3 | 0.76 | -11.98 | -30.19 | -10.76 |
| One area | XS-4 | 0.69 | -11.98 | -31.06 | -6.53 |
| 2nd area | XS-5 | 0.67 | -11.98 | -29.34 | -9.00 |
| 2nd area | XS-6 | 0.73 | -11.98 | -30.81 | -9.12 |
| 2nd area | XS-7 | 0.77 | -11.98 | -31.72 | -8.89 |
| 2nd area | XS-8 | 0.71 | -11.98 | -30.05 | -5.81 |
| 3rd area | XS-9 | 0.75 | -11.98 | -30.50 | -6.55 |
| 3rd area | XS-10 | 0.72 | -11.98 | -28.81 | -8.33 |
| 3rd area | XS-11 | 0.68 | -11.98 | -29.08 | -8.23 |
| 3rd area | XS-12 | 0.74 | -11.98 | -30.53 | -9.68 |
The stable carbon isotope fractionation value of 2 Guiyang general mountain July of table, three region camplotheca acuminata blade assimilation carbon dioxide
Δ a, the stable carbon isotope fractionation value Δ b and heavy carbonate equipment usage f for assimilating heavy carbonateb
2) determination of three compartmentalization perfume (or spice) heavy carbonate equipment usages of Guiyang general mountain July：
Three compartmentalization perfume (or spice) of July on Guiyang general mountain will be grown in as research object, under fair weather, profit
It is used with portable photosynthetic instrument and measures the morning 9 respectively：00-10：The photosynthetic ginseng of the blade of the second expansion leaf on 00 plant shoot
Number；The data of extracellular atmospheric carbon dioxide concentration Ca and intercellular gas concentration lwevel Ci are read from portable photosynthetic instrument, are calculated
The ratio f of plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca, such as table 3；Measure plant simultaneously
The stable carbon isotope of the blade of the first expansion leaf on shoot forms δ13The value δ of CT, such as table 3；It obtains simultaneously by investigation environment
Atmospheric carbon dioxide stable carbon isotope forms δ13The average value δ of CaAnd the Stable Carbon of the rhizosphere soil heavy carbonate of plant is same
Position element composition δ13The value δ of Cb, such as table 3；According to plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration
The ratio f of Ca；The stable carbon isotope fractionation value Δ a for calculating plant leaf blade assimilation carbon dioxide, such as table 4；Computational methods are：Δ
A=4.4 ‰+(D-4.4 ‰) f, wherein the value of D is 29 ‰；Then, the Stable Carbon of calculatingization spiceleaf piece assimilation heavy carbonate
Isotope fractionation value Δ b, such as table 4；Finally, the stable carbon isotope of foundationization spiceleaf piece forms δ13The value δ of CT, air titanium dioxide
Carbon stable carbon isotope forms δ13The average value δ of Ca, rhizosphere soil heavy carbonate stable carbon isotope form δ13The value δ of Cb、
Stable carbon isotope fractionation value Δ a, the Stable Carbon of change spiceleaf piece assimilation heavy carbonic hydrochlorate for changing spiceleaf piece assimilation carbon dioxide are same
The plain fractionation value Δ b in position, calculates plant heavy carbonate equipment usage fb, such as table 4.
The blade intercellular gas concentration lwevel Ci of table 3 Guiyang general mountain July, three compartmentalization perfume and extracellular air two
The stable carbon isotope of the blade of the first expansion leaf on the ratio f of carbonoxide concentration C a, shoot forms δ13The value δ of CT, air
Carbon dioxide stable carbon isotope forms δ13The average value δ of CaAnd the stable carbon isotope of the rhizosphere soil heavy carbonate of plant
Form δ13The value δ of Cb
The stable carbon isotope fractionation value of 4 Guiyang general mountain July of table, three compartmentalization spiceleaf piece assimilation carbon dioxide
Δ a, the stable carbon isotope fractionation value Δ b and heavy carbonate equipment usage f for assimilating heavy carbonateb
| Sample | Plant number | Δa‰ | Δb‰ | fb% |
| One area | HX-1 | 17.44 | 3.14 | 4.2 |
| One area | HX-2 | 18.67 | 4.37 | 10.0 |
| One area | HX-3 | 18.67 | 4.37 | 9.1 |
| 2nd area | HX-4 | 20.64 | 6.34 | 16.5 |
| 2nd area | HX-5 | 20.39 | 6.09 | 9.8 |
| 2nd area | HX-6 | 20.39 | 6.09 | 19.0 |
| 2nd area | HX-7 | 20.14 | 5.84 | 25.9 |
| 2nd area | HX-8 | 20.88 | 6.58 | 21.8 |
| 3rd area | HX-9 | 18.18 | 3.88 | 10.2 |
| 3rd area | HX-10 | 17.93 | 3.63 | 14.3 |
| 3rd area | HX-11 | 18.42 | 4.12 | 21.4 |
| 3rd area | HX-12 | 19.41 | 5.11 | 21.8 |
3) determination of three region camplotheca acuminata heavy carbonate equipment usages of Guiyang general mountain August：
Three region camplotheca acuminatas of August on Guiyang general mountain will be grown in as research object, under fair weather, profit
It is used with portable photosynthetic instrument and measures the morning 9 respectively：00-10：The photosynthetic ginseng of the blade of the second expansion leaf on 00 plant shoot
Number；The data of extracellular atmospheric carbon dioxide concentration Ca and intercellular gas concentration lwevel Ci are read from portable photosynthetic instrument, are calculated
The ratio f of plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca, such as table 5；Measure plant simultaneously
The stable carbon isotope of the blade of the first expansion leaf on shoot forms δ13The value δ of CT, such as table 5；It obtains simultaneously by investigation environment
Atmospheric carbon dioxide stable carbon isotope forms δ13The average value δ of CaAnd the Stable Carbon of the rhizosphere soil heavy carbonate of plant is same
Position element composition δ13The value δ of Cb, such as table 5；According to plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration
The ratio f of Ca；The stable carbon isotope fractionation value Δ a for calculating plant leaf blade assimilation carbon dioxide, such as table 6；Computational methods are：Δ
A=4.4 ‰+(D-4.4 ‰) f, wherein the value of D is 29 ‰；Then, the Stable Carbon of camplotheca acuminata blade assimilation heavy carbonate is calculated
Isotope fractionation value Δ b, such as table 6；Finally, δ is formed according to the stable carbon isotope of camplotheca acuminata blade13The value δ of CT, air titanium dioxide
Carbon stable carbon isotope forms δ13The average value δ of Ca, rhizosphere soil heavy carbonate stable carbon isotope form δ13The value δ of Cb、
Camplotheca acuminata blade assimilates the same position of Stable Carbon of the stable carbon isotope fractionation value Δ a of carbon dioxide, camplotheca acuminata blade assimilation heavy carbonate
Plain fractionation value Δ b calculates plant heavy carbonate equipment usage fb, such as table 6.
The blade intercellular gas concentration lwevel Ci of 5 Guiyang general mountain August of table, three region camplotheca acuminatas and extracellular air two
The stable carbon isotope of the blade of the first expansion leaf on the ratio f of carbonoxide concentration C a, shoot forms δ13The value δ of CT, air
Carbon dioxide stable carbon isotope forms δ13The average value δ of CaAnd the stable carbon isotope of the rhizosphere soil heavy carbonate of plant
Form δ13The value δ of Cb
| Sample | Plant number | f | δa‰ | δT‰ | δb‰ |
| One area | XS-1 | 0.62 | -11.98 | -29.34 | -8.69 |
| One area | XS-2 | 0.60 | -11.98 | -28.77 | -8.62 |
| One area | XS-3 | 0.66 | -11.98 | -28.13 | -9.11 |
| One area | XS-4 | 0.54 | -11.98 | -28.25 | -9.04 |
| One area | XS-5 | 0.68 | -11.98 | -30.62 | -6.18 |
| 2nd area | XS-6 | 0.56 | -11.98 | -29.23 | -10.93 |
| 2nd area | XS-7 | 0.74 | -11.98 | -30.31 | -10.07 |
| 2nd area | XS-8 | 0.65 | -11.98 | -28.76 | -8.35 |
| 2nd area | XS-9 | 0.61 | -11.98 | -26.75 | -7.98 |
| 3rd area | XS-10 | 0.63 | -11.98 | -27.72 | -9.65 |
| 3rd area | XS-11 | 0.67 | -11.98 | -30.07 | -8.49 |
| 3rd area | XS-12 | 0.80 | -11.98 | -30.64 | -10.24 |
The stable carbon isotope fractionation value of 6 Guiyang general mountain August of table, three region camplotheca acuminata blade assimilation carbon dioxide
Δ a, the stable carbon isotope fractionation value Δ b and heavy carbonate equipment usage f for assimilating heavy carbonic hydrochlorateb
| Sample | Plant number | Δa‰ | Δb‰ | fb% |
| One area | XS-1 | 19.65 | 5.35 | 13.0 |
| One area | XS-2 | 19.16 | 4.86 | 13.4 |
| One area | XS-3 | 20.64 | 6.34 | 26.1 |
| One area | XS-4 | 17.68 | 3.38 | 8.2 |
| One area | XS-5 | 21.13 | 6.83 | 12.4 |
| 2nd area | XS-6 | 18.18 | 3.88 | 6.0 |
| 2nd area | XS-7 | 22.60 | 8.30 | 26.3 |
| 2nd area | XS-8 | 20.39 | 6.09 | 20.1 |
| 2nd area | XS-9 | 19.41 | 5.11 | 25.3 |
| 3rd area | XS-10 | 19.90 | 5.60 | 25.0 |
| 3rd area | XS-11 | 20.88 | 6.58 | 15.7 |
| 3rd area | XS-12 | 24.08 | 9.78 | 33.8 |
4) determination of three compartmentalization perfume (or spice) heavy carbonate equipment usages of Guiyang general mountain August：
Three compartmentalization perfume (or spice) of August on Guiyang general mountain will be grown in as research object, under fair weather, profit
It is used with portable photosynthetic instrument and measures the morning 9 respectively：00-10：The photosynthetic ginseng of the blade of the second expansion leaf on 00 plant shoot
Number；The data of extracellular atmospheric carbon dioxide concentration Ca and intercellular gas concentration lwevel Ci are read from portable photosynthetic instrument, are calculated
The ratio f of plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca, such as table 7；Measure plant simultaneously
The stable carbon isotope of the blade of the first expansion leaf on shoot forms δ13The value δ of CT, such as table 7；It obtains simultaneously by investigation environment
Atmospheric carbon dioxide stable carbon isotope forms δ13The average value δ of CaAnd the Stable Carbon of the rhizosphere soil heavy carbonate of plant is same
Position element composition δ13The value δ of Cb, such as table 7；According to plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration
The ratio f of Ca；The stable carbon isotope fractionation value Δ a for calculating plant leaf blade assimilation carbon dioxide, such as table 8；Computational methods are：Δ
A=4.4 ‰+(D-4.4 ‰) f, wherein the value of D is 27 ‰；Then, the Stable Carbon of calculatingization spiceleaf piece assimilation heavy carbonate
Isotope fractionation value Δ b, such as table 8；Finally, the stable carbon isotope of foundationization spiceleaf piece forms δ13The value δ of CT, air titanium dioxide
Carbon stable carbon isotope forms δ13The average value δ of Ca, rhizosphere soil heavy carbonate stable carbon isotope form δ13The value δ of Cb、
The same position of Stable Carbon changed the stable carbon isotope fractionation value Δ a of spiceleaf piece assimilation carbon dioxide, change spiceleaf piece assimilation heavy carbonate
Plain fractionation value Δ b calculates plant heavy carbonate equipment usage fb, such as table 8.
The blade intercellular gas concentration lwevel Ci of 7 Guiyang general mountain August of table, three compartmentalization perfume and extracellular air two
The stable carbon isotope of the blade of the first expansion leaf on the ratio f of carbonoxide concentration C a, shoot forms δ13The value δ of CT, air
Carbon dioxide stable carbon isotope forms δ13The average value δ of CaAnd the stable carbon isotope of the rhizosphere soil heavy carbonate of plant
Form δ13The value δ of Cb
| Sample | Plant number | f | δa‰ | δT‰ | δb‰ |
| One area | HX-1 | 0.60 | -11.98 | -28.70 | -6.94 |
| One area | HX-2 | 0.65 | -11.98 | -26.25 | -10.49 |
| One area | HX-3 | 0.58 | -11.98 | -28.49 | -7.98 |
| 2nd area | HX-4 | 0.59 | -11.98 | -28.28 | -10.10 |
| 2nd area | HX-5 | 0.59 | -11.98 | -28.46 | -10.07 |
| 2nd area | HX-6 | 0.64 | -11.98 | -29.08 | -11.49 |
| 2nd area | HX-7 | 0.72 | -11.98 | -30.60 | -8.35 |
| 2nd area | HX-8 | 0.68 | -11.98 | -29.46 | -7.98 |
| 2nd area | HX-9 | 0.69 | -11.98 | -28.58 | -6.01 |
| 3rd area | HX-10 | 0.68 | -11.98 | -28.92 | -7.53 |
| 3rd area | HX-11 | 0.65 | -11.98 | -27.80 | -9.04 |
| 3rd area | HX-12 | 0.62 | -11.98 | -29.92 | -10.32 |
| 3rd area | HX-13 | 0.73 | -11.98 | -27.95 | -9.10 |
The stable carbon isotope fractionation value of 8 Guiyang general mountain August of table, three compartmentalization spiceleaf piece assimilation carbon dioxide
Δ a, the stable carbon isotope fractionation value Δ b and heavy carbonate equipment usage f for assimilating heavy carbonateb
The implementation result of the present invention is as follows：
Different plants is can be seen that in different regions, same plant in difference from the result of table 2, table 4, table 6, table 8
In the different time of the same area, heavy carbonate equipment usage is all different for region, even same plant.Conclude table 2, table 4, table
6, the result of table 8 obtains table 9.It can be seen that the steady of different plant assimilating carbon dioxide from table 2, table 4, table 6, table 8 and table 9
Determine that carbon isotope fractionation value is significantly different, but same plant is in the stable carbon isotope fractionation for assimilating carbon dioxide different months
Value difference is different smaller, and the stable carbon isotope fractionation value of the assimilation carbon dioxide of all material is approached with 20 ‰；Different plants
Assimilate heavy carbonate in the different time of the same area in different zones, even same plant in different regions, same plant
Stable carbon isotope fractionation value be all different, this illustrate plant heavy carbonate utilization have higher variability, karst
Adaptive plant may be exactly to adapt to environment using flexible and changeable heavy carbonate.Meanwhile from table 2, table 4, table 6, table 8 and
In table 9 it can also be seen that three region camplotheca acuminata heavy carbonate equipment usages of Guiyang general mountain July from 4.6% to
21.7%, average value 13.6% changes fragrant heavy carbonate equipment usage from 4.2% to 25.9%, average value 15.3%.Kweiyang
Three region camplotheca acuminata heavy carbonate equipment usages of city's general mountain August are from 6.0% to 33.8%, average value 18.8%, change fragrant
Heavy carbonate equipment usage is from 3.0% to 30.5%, average value 13.7%.Pair of these results of camplotheca acuminata and our laboratories
It is similar to the result obtained by tagging culture technique.It can thus be seen that plant is also demonstrated by terms of inorganic carbon utilization
The feature namely plant for adapting to diversified environment change the utilization of inorganic carbon according to environment, to adapt to environment.
9 Guiyang general mountain seven of table, three region camplotheca acuminatas of August, the same position of Stable Carbon for changing spiceleaf piece assimilation carbon dioxide
Plain fractionation value Δ a, the stable carbon isotope fractionation value Δ b and heavy carbonate equipment usage f for assimilating heavy carbonateb
Claims (6)
1. a kind of method obtaining plant heavy carbonate equipment usage under outdoor habitatss, it is characterised in that including：
Step 1, the blade of second expansion leaf of selection in the environment of being investigated on growing plants shoot, in fair weather
Under, the morning 9 is measured respectively using portable photosynthetic instrument：00-10：00 photosynthetic parameters；
Step 2 obtains the number of extracellular atmospheric carbon dioxide concentration Ca and intercellular gas concentration lwevel Ci from portable photosynthetic instrument
According to the ratio f of calculating plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca；
Step 3, the blade of first expansion leaf of selection in the environment of being investigated on growing plants shoot, utilizes conventional side
The stable carbon isotope that method measures plant leaf blade forms δ13The value of C, is denoted as δT；
Step 4 obtains form δ by investigation ambient air carbon dioxide stable carbon isotope according to a conventional method13The average value of C, note
For δa；
Step 5 measures the Stable Carbon of the rhizosphere soil heavy carbonate of growing plants in the environment of being investigated according to a conventional method
Isotopics δ13The value of C, is denoted as δb；
Step 6 is calculated according to the ratio f of plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca
The stable carbon isotope fractionation value Δ a of growing plants blade assimilation carbon dioxide in the environment of being investigated；
Step 7, the stable carbon isotope fractionation value according to growing plants blade assimilation carbon dioxide in the environment of being investigated
Δ a calculates the stable carbon isotope fractionation value Δ b of growing plants blade assimilation heavy carbonate in the environment of being investigated；
Step 8, the stable carbon isotope according to growing plants blade in the environment of being investigated form δ13The value δ of CT, examined
Examine ambient air carbon dioxide stable carbon isotope composition δ13The average value δ of Ca, in the environment of being investigated growing plants
The stable carbon isotope of rhizosphere soil heavy carbonate forms δ13The value δ of Cb；Growing plants blade is same in the environment of being investigated
Change the stable carbon isotope fractionation value Δ a of carbon dioxide, growing plants blade assimilation heavy carbonate in the environment of being investigated
Stable carbon isotope fractionation value Δ b, calculate plant heavy carbonate equipment usage fb。
2. a kind of method obtaining plant heavy carbonate equipment usage under outdoor habitatss according to claim 1, feature
It is：In the first step, the blade of the second expansion leaf on the plant shoot is according to principle from top to bottom, with new
It is the first fully expanded leaves that the leaf completely and being fully deployed just has been developed on branch, and so on；Respectively the second fully expanded leaves,
Third fully expanded leaves.
3. a kind of method characteristic obtaining plant heavy carbonate equipment usage under outdoor habitatss according to claim 1 exists
In：The ratio f of plant leaf blade intercellular gas concentration lwevel Ci and extracellular atmospheric carbon dioxide concentration Ca described in step 2, meter
Calculation method is：
4. a kind of method obtaining plant heavy carbonate equipment usage under outdoor habitatss according to claim 1, feature
It is：The stable carbon isotope point of the assimilation carbon dioxide of growing plants blade in the environment of being investigated described in step 6
The computational methods for evaporating value Δ a are：Δ a=4.4 ‰+(D-4.4 ‰) f, wherein to inorganic carbon when D is Rubsico carboxylations
Fractionation, the different values according to plant are different, and ranging from 27 ‰~29 ‰.
5. a kind of method obtaining plant heavy carbonate equipment usage under outdoor habitatss according to claim 1, feature
It is：In the 7th step, the stable carbon isotope point of growing plants blade assimilation heavy carbonate in the environment of being investigated
The computational methods for evaporating value are：Δ b=Δs a-4.4 ‰ -9.9 ‰.
6. a kind of method obtaining plant heavy carbonate equipment usage under outdoor habitatss according to claim 1, feature
It is：Plant heavy carbonate equipment usage f described in step 8bComputational methods be：
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| CN109470826A (en) * | 2018-12-26 | 2019-03-15 | 中国科学院地球化学研究所 | A kind of method of plant metabolism water equipment usage and practical water requirement under acquisition outdoor habitatss |
| CN112379054A (en) * | 2020-11-12 | 2021-02-19 | 中国科学院地球化学研究所 | Method for judging proper amplitude and optimal concentration of plant growth bicarbonate |
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