CN108244332A - The preparation method of Gallus domesticlus brisson Gly-His-Lys and Gallus domesticlus brisson Gly-His-Lys - Google Patents
The preparation method of Gallus domesticlus brisson Gly-His-Lys and Gallus domesticlus brisson Gly-His-Lys Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/02—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from meat
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
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Abstract
The invention discloses a kind of preparation method of Gallus domesticlus brisson Gly-His-Lys and Gallus domesticlus brisson Gly-His-Lys.The preparation method includes being pre-processed Gallus domesticlus brisson, cutting and defibrination, obtains Gallus domesticlus brisson slurries, and Gallus domesticlus brisson slurries are precooked, are digested and enzyme deactivation, then filtered, be concentrated in vacuo and dry, obtains Gallus domesticlus brisson Gly-His-Lys.The defects of preparation method of the present invention overcomes traditional Gallus domesticlus brisson application method enriches the diversity of Gallus domesticlus brisson small-molecular peptides product preparation, and Gallus domesticlus brisson Gly-His-Lys obtained are full of nutrition, easily digest and assimilate, and are with a wide range of applications and important social value.
Description
Technical field
The present invention relates to the preparing technical fields of biologically active peptide, and in particular to a kind of preparation method of Gallus domesticlus brisson Gly-His-Lys and crow
Chicken Gly-His-Lys.
Background technology
Gallus domesticlus brisson is the unique integration of drinking and medicinal herbs chicken kind in China, not only can individually eat but also can make up a prescription into compound, has filling liver kidney, benefit
Qi and blood, reducing the asthenic fever control pain in the chest and abdomen and other effects.It is indoles type containing Determination of multiple metal elements such as Fe, Mg, Zn, Ca in melanin of black-bone chicken
Irregular high polymer and indoles precursor substance.Gallus domesticlus brisson has the function of to clean blood of human body and removes rubbish, energy in blood
Immune function of human body is adjusted, property disease deficient to paramenia caused by deficiency of qi and blood and the elderly has fine benefiting action.Crow
Chicken contains a large amount of vitamin A, trace elements of selenium, they, which have, removes interior free yl, anti-aging and inhibition growth of cancer cells
The effect of.In recent years, Gallus domesticlus brisson has started one research heat because of its special nutritious tonifying and medical value in medical health field
Tide.But traditional concocting method is that chicken is directly used as medicine, and can not change the macromolecular structure of original protein, human body is inhaled
Yield is low, and active ingredient can not be played and be utilized completely.Biologically active peptide be it is beneficial to the vital movement of living organism or
Peptides with physiological action, though there is scientific research personnel that Gallus domesticlus brisson is prepared into Gallus domesticlus brisson active peptide using enzymatic hydrolysis technique,
But effect is general, and on the whole, and the research of this part is still not very more, can not meet existing to the Gallus domesticlus brissons small molecule such as Gallus domesticlus brisson polypeptide
The active demand of product.Therefore, the preparation process for developing new Gallus domesticlus brisson small molecule product is extremely urgent.
Invention content
The technical problem to be solved by the present invention is to overcome the deficiencies in the prior art, provide a kind of nutritive value height, easily inhale
The system that receive, the diversity of Gallus domesticlus brisson small-molecular peptides product preparation can be enriched and can be widely used for the Gallus domesticlus brisson Gly-His-Lys of special doctor's field of food
Preparation Method and Gallus domesticlus brisson Gly-His-Lys.
In order to solve the above technical problems, the present invention uses following technical scheme：
A kind of preparation method of Gallus domesticlus brisson Gly-His-Lys, includes the following steps：Gallus domesticlus brisson is pre-processed, cutting and defibrination, obtained
Gallus domesticlus brisson slurries are precooked, are digested and enzyme deactivation by Gallus domesticlus brisson slurries, then filtered, be concentrated in vacuo and dry, obtain Gallus domesticlus brisson peptide
Powder.
In the preparation method of above-mentioned Gallus domesticlus brisson Gly-His-Lys, it is preferred that the process of the enzymolysis is as follows：Gallus domesticlus brisson slurry after precooking
Papain is first added in liquid and carries out first time enzymolysis, compound protease is then added in and carries out second of enzymolysis, add wind
Taste protease carries out third time enzymolysis, wherein, for the first time the temperature of enzymolysis, second enzymolysis and third time enzymolysis be 45 DEG C~
60℃。
In the preparation method of above-mentioned Gallus domesticlus brisson Gly-His-Lys, it is preferred that the quality of the papain is adds in Papain
0.01%~0.1% of slurries gross mass after enzyme, the enzyme activity of the papain is the U/g of 400,000 U/g~800,000, described
The quality of compound protease be add in compound protease after slurries gross mass 0.01%~0.2%, the compound protease
Enzyme activity be the U/g of 200,000 U/g~400,000, the quality of the flavor protease be the slurries gross mass after addition flavor protease
0.01%~0.1%, the enzyme activity of the flavor protease is the U/g of 200,000 U/g~400,000；
And/or the time of the first time enzymolysis is 1h~4h, the time of second of enzymolysis is 1h~4h, described
The time of third time enzymolysis is 1h~3h.
In the preparation method of above-mentioned Gallus domesticlus brisson Gly-His-Lys, it is preferred that the temperature of the enzyme deactivation is 90 DEG C~100 DEG C, described to go out
The time of enzyme is 5min~20min.
In the preparation method of above-mentioned Gallus domesticlus brisson Gly-His-Lys, it is preferred that it is described vacuum concentration include the first stage be concentrated in vacuo with
Second stage is concentrated in vacuo, and the temperature that the first stage is concentrated in vacuo is 60 DEG C~70 DEG C, and the first stage is concentrated in vacuo
Time for 60min~90min, the temperature that the second stage is concentrated in vacuo is 70 DEG C~80 DEG C, the second stage vacuum
The time of concentration is 30min~60min.
In the preparation method of above-mentioned Gallus domesticlus brisson Gly-His-Lys, it is preferred that before the vacuum concentration, first add maltodextrin.
In the preparation method of above-mentioned Gallus domesticlus brisson Gly-His-Lys, it is preferred that the drying for spray drying or vacuum freeze drying, institute
The temperature for stating spray drying is 180 DEG C~200 DEG C, and the temperature of the vacuum freeze drying is -10 DEG C~-50 DEG C, and vacuum degree is
1.3Pa~13Pa.
In the preparation method of above-mentioned Gallus domesticlus brisson Gly-His-Lys, it is preferred that the defibrination is carried out using colloid mill grinding, the colloid
The gap of mill grinding is 0.1mm~1mm；
And/or the temperature precooked is 90 DEG C~120 DEG C, the time precooked is 5min~20min.
In the preparation method of above-mentioned Gallus domesticlus brisson Gly-His-Lys, it is preferred that the filtering is sieved using 40~60 mesh.
The technical concept total as one, the present invention also provides Gallus domesticlus brisson Gly-His-Lys made from a kind of above-mentioned preparation method.
In the preparation process of Gallus domesticlus brisson Gly-His-Lys of the present invention, it is highly preferred that the quality of the papain is adds in pawpaw egg
0.01%~0.08% of slurries gross mass after enzyme in vain, the quality of the compound protease are the slurry added in after compound protease
The 0.01%~0.06% of liquid gross mass, the quality of the flavor protease are the slurries gross mass added in after flavor protease
0.01%~0.08%.
Compared with prior art, the advantage of the invention is that：
The preparation method is that Gallus domesticlus brisson is made small-molecular peptides using biotechnology newly developed, this method first will be black
Chicken is milled into Gallus domesticlus brisson slurries, then carries out three through specific enzyme preparation papain, compound protease and flavor protease respectively
Small molecule Gallus domesticlus brisson Gly-His-Lys are prepared by biological enzymolysis technology in secondary enzymolysis, which is enzyme process polypeptide, can without polypeptide hormone
Can caused by reject and allergic reaction, and without acidolysis, the chemical side effect of alkaline hydrolysis, it have human endogenous's peptide sample activity and
Function is the substrate of protein degradation in human body and protein metabolism and forms the important substance of human body protein.Small peptide
It is more easy to compared with amino acid, faster absorption of human body and utilization.Using a variety of enzyme process technologies, protein is carried out effectively, to be thoroughly catalyzed,
Acquisition quality is good, peptide molecule chain is small, and amino acid forms reasonable, stable effect, reliable in quality micromolecule active polypeptide (molecule matter
Amount is mostly in 1000Da hereinafter, more than 80% accounting).Enzyme process polypeptide simulates human body synthesis polypeptide pattern, with biological enzyme egg
White matter obtains polypeptide, has adapted to low-carbon economy and environmentally protective requirement, mild, environmentally friendly compared with acid system, alkaline process, electrical method.Produce work
Skill is simple, small investment, quick, suitable industrialized production.
Gallus domesticlus brisson Gly-His-Lys produced by the present invention contain 16 kinds of amino acid, cannot especially be synthesized containing human body itself or aggregate velocity
Human body needs cannot be met, it is necessary to the amino acid absorbed from food, such as lysine (Lys), tryptophan (Trp), phenylalanine
(Phe), methionine (Met), threonine (Thr), isoleucine (Ile), leucine (Leu), valine (Val) etc., wherein
Contained essential amino acid accounts for the 38.70% of total amino acid in Gallus domesticlus brisson Gly-His-Lys.Gallus domesticlus brisson Gly-His-Lys mineral rich content, and contain 6
Kind vitamin and a large amount of protein, after body operation or puerpera eats in puerperium, can myogenic promoting blood circulation and accelerating wound healing.
The preparation method of the present invention changes traditional Gallus domesticlus brisson application method, and it is easy to operate, product effect is good, and abundant
The preparation diversity of Gallus domesticlus brisson small-molecular peptides product, after Gallus domesticlus brisson is made Gallus domesticlus brisson Gly-His-Lys, has the characteristics that：(1) it is not required to digest,
Directly absorb；(2) it absorbs quick；(3) polypeptide is not destroyed when absorbing；It (4) can active absorption；(5) it is preferentially absorbed by the body；
(6) it does not need to expend body energy, alimentary canal particularly gastrointestinal function burden will not be increased；(7) polypeptide shows to carry in human body
Body acts on, the nutriment that can be eaten mediocrity, particularly calcium etc. the trace element beneficial to human body, absorption, adhesion, loading
On ontology；(8) polypeptide can play means of transport in human body.
Specific embodiment
Below in conjunction with specific preferred embodiment, the invention will be further described, but not thereby limiting the invention
Protection domain.
Material and instrument employed in following embodiment are commercially available.Wherein, papain is purchased from Nanning Pang Bosheng
Object Engineering Co., Ltd, compound protease believe (China) Bioisystech Co., Ltd purchased from Novi, and flavor protease is purchased from Novi
Believe (China) Bioisystech Co., Ltd, but not limited to this.
Embodiment 1
A kind of preparation method of the Gallus domesticlus brisson Gly-His-Lys of the present invention, includes the following steps：
Pre-processed using fresh (jelly) Gallus domesticlus brisson as raw material, cutting and defibrination, the gap of colloid mill grinding that defibrination uses for
0.5mm obtains Gallus domesticlus brisson slurries.Gallus domesticlus brisson slurries are precooked 10 minutes at 100 DEG C, enzyme activity is then added in into Gallus domesticlus brisson slurries is
The papain enzymolysis 2h of 600000 U/g, the quality of papain are add papain rear slurry gross mass 0.04%,
It adds the compound protease that enzyme activity is 400,000 U/g to digest 4 hours, the quality of compound protease is starches after adding compound protease
The 0.03% of liquid gross mass adds the flavor protease that enzyme activity is 400,000 U/g and digests 2 hours, the quality of flavor protease
To add the 0.04% of flavor protease rear slurry gross mass, the temperature digested three times is 50 DEG C.After the completion of enzymolysis, at 95 DEG C
Enzyme deactivation 10 minutes.50 mesh sieve is crossed after enzyme deactivation to be filtered, and maltodextrin (do not add can also) is added into filtrate, carries out vacuum
Concentration is concentrated in vacuo in two stages, and first stage temperature is 65 DEG C, time 75min, and second stage temperature is 80 DEG C,
Time is 45min, is concentrated after being spray-dried at 190 DEG C or vacuum freeze drying, vacuum 5 are carried out at -10 DEG C
Pa obtains Gallus domesticlus brisson Gly-His-Lys, to digest bioactivity peptides products, can be packed.
The amino acid content of Gallus domesticlus brisson Gly-His-Lys (Gallus domesticlus brisson polypeptide) component that the present embodiment is prepared is determined below,
The results are shown in Table 1.
Amino acid and content in 1 Gallus domesticlus brisson polypeptide of table
| Amino acid name | Content (g/100g) | Amino acid name | Content (g/100g) |
| Asparatate | 8.108 | Leucine | 6.446 |
| Arginine | 6.956 | Isoleucine | 3.611 |
| Glycine | 8.728 | Phenylalanine | 3.832 |
| Threonine | 3.921 | Histidine | 3.854 |
| Proline | 5.184 | Lysine | 6.911 |
| Alanine | 6.579 | Tyrosine | 2.658 |
| Valine | 4.142 | Glutamic acid | 13.823 |
| Methionine | 2.215 | Serine | 7.620 |
In above-mentioned Gallus domesticlus brisson polypeptide, after measured, molecular weight (note：HPLC area normalization methods measure) be below 2000 dalton
94.60%, it is 50% below 800 dalton of molecular weight, it is seen that effectively to Gallus domesticlus brisson point, big sub- albumen has carried out small molecule to the present invention
Conversion and extraction, can directly be absorbed by human body is actively quick, substantially alleviate alimentary canal particularly stomach and intestine without gastro-intestinal digestion
The burden of function, meanwhile, polypeptide can be by micro- beneficial to human body such as nutriment particularly calcium that mediocrity is eaten as carrier
Secondary element is adsorbed, adhesion and is loaded on ontology, plays effective translocation of nutriment in human body.
Embodiment 2
A kind of preparation method of the Gallus domesticlus brisson Gly-His-Lys of the present invention, includes the following steps：
Pre-processed using fresh (jelly) Gallus domesticlus brisson as raw material, cutting and defibrination, the gap of colloid mill grinding that defibrination uses for
0.2mm obtains Gallus domesticlus brisson slurries.Gallus domesticlus brisson slurries are precooked 15 minutes at 95 DEG C, enzyme activity is then added in into Gallus domesticlus brisson slurries is
The papain enzymolysis 3h of 600000 U/g, the quality of papain for slurries gross mass (including newly plus enzyme, similarly hereinafter)
0.06%, it adds the compound protease that enzyme activity is 400,000 U/g and digests 2h, the quality of compound protease is slurries gross mass
0.08%, it adds the flavor protease that enzyme activity is 400,000 U/g and digests 4h, the quality of flavor protease is slurries gross mass
0.02%, the temperature digested three times is 50 DEG C.After the completion of enzymolysis, enzyme deactivation 15 minutes at 90 DEG C.After enzyme deactivation cross 50 mesh sieve into
Row filtering, maltodextrin (do not add can also) is added into filtrate, is concentrated in vacuo, and is concentrated in vacuo in two stages, the
One phase temperature is 60 DEG C, time 85min, and second stage temperature is 75 DEG C, and time 55min is concentrated after at 180 DEG C
It is spray-dried or vacuum freeze drying is carried out at -20 DEG C, 10 pa of vacuum obtains Gallus domesticlus brisson Gly-His-Lys.
Embodiment 3
A kind of preparation method of the Gallus domesticlus brisson Gly-His-Lys of the present invention, includes the following steps：
Pre-processed using fresh (jelly) Gallus domesticlus brisson as raw material, cutting and defibrination, the gap of colloid mill grinding that defibrination uses for
1mm obtains Gallus domesticlus brisson slurries.Gallus domesticlus brisson slurries are precooked 10 minutes at 120 DEG C, it is 80 that enzyme activity is then added in into Gallus domesticlus brisson slurries
The papain enzymolysis 4h of ten thousand U/g, the quality of papain for slurries gross mass (including newly plus enzyme, similarly hereinafter)
0.05%, it adds the compound protease that enzyme activity is 200,000 U/g and digests 2 hours, the quality of compound protease is the total matter of slurries
The 0.1% of amount adds the flavor protease that enzyme activity is 300,000 U/g and digests 3 hours, and the quality of flavor protease is total for slurries
The 0.08% of quality, the temperature digested three times are 45 DEG C.After the completion of enzymolysis, enzyme deactivation 10 minutes at 95 DEG C.40 are crossed after enzyme deactivation
Mesh sieve is filtered, and maltodextrin (do not add can also) is added into filtrate, is concentrated in vacuo, and vacuum concentration is divided to two ranks
Section, first stage temperature be 70 DEG C, time 65min, second stage temperature be 80 DEG C, time 45min, concentrate after
It is spray-dried at 190 DEG C or vacuum freeze drying is carried out at -10 DEG C, 5 pa of vacuum obtains Gallus domesticlus brisson Gly-His-Lys.
Embodiment 4
A kind of preparation method of the Gallus domesticlus brisson Gly-His-Lys of the present invention, includes the following steps：
Pre-processed using fresh (jelly) Gallus domesticlus brisson as raw material, cutting and defibrination, the gap of colloid mill grinding that defibrination uses for
0.5mm obtains Gallus domesticlus brisson slurries.Gallus domesticlus brisson slurries are precooked 10 minutes at 100 DEG C, enzyme activity is then added in into Gallus domesticlus brisson slurries is
The papain enzymolysis 4h of 500000 U/g, the quality of papain for slurries gross mass (including newly plus enzyme, similarly hereinafter)
0.02%, it adds the compound protease that enzyme activity is 400,000 U/g and digests 3 hours, the quality of compound protease is the total matter of slurries
The 0.08% of amount adds the flavor protease that enzyme activity is 400,000 U/g and digests 2 hours, and the quality of flavor protease is slurries
The 0.08% of gross mass, the temperature digested three times are 50 DEG C.After the completion of enzymolysis, enzyme deactivation 10 minutes at 95 DEG C.Mistake after enzyme deactivation
50 mesh sieve is filtered, and maltodextrin (do not add can also) is added into filtrate, is concentrated in vacuo, and vacuum concentration is divided to two
Stage, first stage temperature be 60 DEG C, time 85min, second stage temperature be 80 DEG C, time 50min, concentrate after
It is spray-dried at 180 DEG C or vacuum freeze drying is carried out at -15 DEG C, 3 pa of vacuum obtains Gallus domesticlus brisson Gly-His-Lys.
Embodiment 5
A kind of preparation method of the Gallus domesticlus brisson Gly-His-Lys of the present invention, includes the following steps：
Pre-processed using fresh (jelly) Gallus domesticlus brisson as raw material, cutting and defibrination, the gap of colloid mill grinding that defibrination uses for
0.5mm obtains Gallus domesticlus brisson slurries.Gallus domesticlus brisson slurries are precooked 10 minutes at 100 DEG C, enzyme activity is then added in into Gallus domesticlus brisson slurries is
The papain enzymolysis 1h of 600000 U/g, the quality of papain for slurries gross mass (including newly plus enzyme, similarly hereinafter)
0.1%, it adds the compound protease that enzyme activity is 400,000 U/g and digests 2 hours, the quality of compound protease is adds compound protein
The 0.15% of enzyme rear slurry gross mass adds the flavor protease that enzyme activity is 400,000 U/g and digests 1 hour, flavor protease
Quality be plus flavor protease rear slurry gross mass 0.1%, the temperature digested three times is 55 DEG C.After the completion of enzymolysis, in
Enzyme deactivation 10 minutes at 95 DEG C.50 mesh sieve is crossed after enzyme deactivation to be filtered, and maltodextrin (do not add can also) is added into filtrate, into
Row is concentrated in vacuo, and is concentrated in vacuo in two stages, and first stage temperature is 65 DEG C, time 75min, and second stage temperature is
It 80 DEG C, time 45min, concentrates after being spray-dried at 190 DEG C or vacuum freeze drying being carried out at -10 DEG C, very
Empty 5 pas, obtain Gallus domesticlus brisson Gly-His-Lys.
In above-described embodiment 2~5, the molecular weight (being measured using HPLC area normalization methods) for the Gallus domesticlus brisson Gly-His-Lys being prepared
Below 2000 dalton more than 94%, below 800 dalton of molecular weight more than 50%, the Gallus domesticlus brisson Gly-His-Lys are substantially with small
Molecular forms exist, and the amino acid containing various needed by human body is easily digested, can directly be absorbed, and infiltration rate is quickly, inhale
During receipts, polypeptide is not easy to be destroyed, and does not need to expend body energy, substantially alleviates the negative of alimentary canal particularly gastrointestinal function
Load, meanwhile, polypeptide as carrier can by nutriment particularly calcium that mediocrity is eaten etc. it is beneficial to human body it is micro- into
Row absorption and is loaded on ontology adhesion, plays effective translocation of nutriment in human body.
The above described is only a preferred embodiment of the present invention, not make limitation in any form to the present invention.Though
So the present invention is disclosed as above with preferred embodiment, however is not limited to the present invention.It is any to be familiar with those skilled in the art
Member, in the case where not departing from the Spirit Essence of the present invention and technical solution, all using in the methods and techniques of the disclosure above
Appearance makes technical solution of the present invention many possible changes and modifications or is revised as the equivalent embodiment of equivalent variations.Therefore,
Every content without departing from technical solution of the present invention, technical spirit according to the present invention are made to the above embodiment any simple
Modification, equivalent replacement, equivalence changes and modification, still fall within technical solution of the present invention protection in the range of.
Claims (10)
1. a kind of preparation method of Gallus domesticlus brisson Gly-His-Lys, which is characterized in that include the following steps：Gallus domesticlus brisson is pre-processed, cutting and
Defibrination obtains Gallus domesticlus brisson slurries, and Gallus domesticlus brisson slurries are precooked, are digested and enzyme deactivation, then filtered, be concentrated in vacuo and dry, obtains
To Gallus domesticlus brisson Gly-His-Lys.
2. the preparation method of Gallus domesticlus brisson Gly-His-Lys according to claim 1, which is characterized in that the process of the enzymolysis is as follows：It will
Papain is first added in Gallus domesticlus brisson slurries after precooking and carries out first time enzymolysis, compound protease is then added in and carries out second
Enzymolysis adds flavor protease and carries out third time enzymolysis, wherein, what first time enzymolysis, second of enzymolysis and third time digested
Temperature is 45 DEG C~60 DEG C.
3. the preparation method of Gallus domesticlus brisson Gly-His-Lys according to claim 2, which is characterized in that the quality of the papain is
Add in 0.01%~0.1% of the slurries gross mass after papain, the enzyme activity of the papain for 400,000 U/g~
800000 U/g, the quality of the compound protease are add in the slurries gross mass after compound protease 0.01%~0.2%, institute
The enzyme activity of compound protease is stated as the U/g of 200,000 U/g~400,000, after the quality of the flavor protease is adds in flavor protease
Slurries gross mass 0.01%~0.1%, the enzyme activity of the flavor protease is the U/g of 200,000 U/g~400,000；
And/or the time of first time enzymolysis is 1h~4h, time of second of enzymolysis is 1h~4h, the third
The time of secondary enzymolysis is 1h~3h.
4. the preparation method of Gallus domesticlus brisson Gly-His-Lys described in any one of claim 1 to 3, which is characterized in that the enzyme deactivation
Temperature is 90 DEG C~100 DEG C, and the time of the enzyme deactivation is 5min~20min.
5. the preparation method of Gallus domesticlus brisson Gly-His-Lys described in any one of claim 1 to 3, which is characterized in that the vacuum is dense
Contracting includes first stage vacuum concentration and second stage is concentrated in vacuo, and the temperature that the first stage is concentrated in vacuo is 60 DEG C~70
DEG C, the time that the first stage is concentrated in vacuo is 60min~90min, and the temperature that the second stage is concentrated in vacuo is 70 DEG C
~80 DEG C, the time that the second stage is concentrated in vacuo is 30min~60min.
6. the preparation method of Gallus domesticlus brisson Gly-His-Lys according to claim 5, which is characterized in that before the vacuum concentration, first add
Maltodextrin.
7. the preparation method of Gallus domesticlus brisson Gly-His-Lys described in any one of claim 1 to 3, which is characterized in that the drying is
Spray drying or vacuum freeze drying, the temperature of the spray drying is 180 DEG C~200 DEG C, the temperature of the vacuum freeze drying
It is -10 DEG C~-50 DEG C to spend, and vacuum degree is 1.3Pa~13Pa.
8. the preparation method of Gallus domesticlus brisson Gly-His-Lys described in any one of claim 1 to 3, which is characterized in that the defibrination is adopted
It is carried out with colloid mill grinding, the gap of the colloid mill grinding is 0.1mm~1mm；
And/or the temperature precooked is 90 DEG C~120 DEG C, the time precooked is 5min~20min.
9. the preparation method of Gallus domesticlus brisson Gly-His-Lys described in any one of claim 1 to 3, which is characterized in that the filtering is adopted
It is sieved with 40~60 mesh.
10. a kind of Gallus domesticlus brisson Gly-His-Lys as made from preparation method according to any one of claims 1 to 9.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1802982A (en) * | 2006-01-23 | 2006-07-19 | 福州大学 | Novel process for producing calcium enriched animal hydrolyzed protein using complex enzyme hydrolyzing chicken bone mud |
| US20070077333A1 (en) * | 2003-09-19 | 2007-04-05 | Masamichi Maeda | Process for producing protein hydrolysate and protein hydrolysate |
| CN102618612A (en) * | 2012-04-06 | 2012-08-01 | 南昌大学 | Preparation method for black-bone chicken peptide for chemotherapy, attenuation and synergia and application of black-bone chicken peptide |
| CN107523602A (en) * | 2017-10-14 | 2017-12-29 | 江西倍得力生物工程有限公司 | A kind of extracting method of black-bone chicken active peptide |
-
2018
- 2018-03-06 CN CN201810184467.4A patent/CN108244332A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070077333A1 (en) * | 2003-09-19 | 2007-04-05 | Masamichi Maeda | Process for producing protein hydrolysate and protein hydrolysate |
| CN1802982A (en) * | 2006-01-23 | 2006-07-19 | 福州大学 | Novel process for producing calcium enriched animal hydrolyzed protein using complex enzyme hydrolyzing chicken bone mud |
| CN102618612A (en) * | 2012-04-06 | 2012-08-01 | 南昌大学 | Preparation method for black-bone chicken peptide for chemotherapy, attenuation and synergia and application of black-bone chicken peptide |
| CN107523602A (en) * | 2017-10-14 | 2017-12-29 | 江西倍得力生物工程有限公司 | A kind of extracting method of black-bone chicken active peptide |
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