CN108239662A - Method for producing ethanol by fermenting manioc waste - Google Patents
Method for producing ethanol by fermenting manioc waste Download PDFInfo
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- CN108239662A CN108239662A CN201611227114.5A CN201611227114A CN108239662A CN 108239662 A CN108239662 A CN 108239662A CN 201611227114 A CN201611227114 A CN 201611227114A CN 108239662 A CN108239662 A CN 108239662A
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 191
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 title claims abstract description 135
- 239000002699 waste material Substances 0.000 title claims description 99
- 238000004519 manufacturing process Methods 0.000 title abstract description 10
- 241000658379 Manihot esculenta subsp. esculenta Species 0.000 title 1
- 240000003183 Manihot esculenta Species 0.000 claims abstract description 134
- 238000000855 fermentation Methods 0.000 claims abstract description 46
- 230000004151 fermentation Effects 0.000 claims abstract description 42
- 239000002002 slurry Substances 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 230000001954 sterilising effect Effects 0.000 claims abstract description 14
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 11
- 102000004190 Enzymes Human genes 0.000 claims abstract description 10
- 229940088598 enzyme Drugs 0.000 claims abstract description 10
- 108010059892 Cellulase Proteins 0.000 claims abstract description 9
- 108090000790 Enzymes Proteins 0.000 claims abstract description 9
- 229940106157 cellulase Drugs 0.000 claims abstract description 9
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 8
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 8
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 7
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 7
- 229940024171 alpha-amylase Drugs 0.000 claims abstract description 7
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 238000010564 aerobic fermentation Methods 0.000 claims abstract description 3
- 235000019441 ethanol Nutrition 0.000 claims description 83
- 238000000034 method Methods 0.000 claims description 48
- 238000012262 fermentative production Methods 0.000 claims description 26
- 229920002472 Starch Polymers 0.000 claims description 17
- 239000008107 starch Substances 0.000 claims description 17
- 235000019698 starch Nutrition 0.000 claims description 17
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 239000000654 additive Substances 0.000 claims description 7
- 230000000996 additive effect Effects 0.000 claims description 7
- 108010089934 carbohydrase Proteins 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 4
- 239000002023 wood Substances 0.000 claims description 4
- 238000004821 distillation Methods 0.000 claims description 2
- 239000000835 fiber Substances 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 239000004382 Amylase Substances 0.000 claims 1
- 108010065511 Amylases Proteins 0.000 claims 1
- 102000013142 Amylases Human genes 0.000 claims 1
- 235000019418 amylase Nutrition 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 239000000843 powder Substances 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 3
- 238000010411 cooking Methods 0.000 abstract 1
- 229960004756 ethanol Drugs 0.000 description 47
- 239000002994 raw material Substances 0.000 description 15
- 239000012530 fluid Substances 0.000 description 9
- 239000002893 slag Substances 0.000 description 5
- 239000000446 fuel Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 229920005610 lignin Polymers 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
the invention provides a method for producing ethanol by fermenting cassava dregs, which comprises the following steps of crushing the cassava dregs, adding water to prepare cassava dreg slurry, adjusting pH, adding ammonium sulfate, sterilizing by high-pressure steam, cooling, adding cellulase under the aseptic operation condition, simultaneously adding brewing active dry yeast, sequentially performing aerobic fermentation and anaerobic fermentation, adding the cassava dregs in batches during fermentation, adding cassava powder, adding α -amylase, adding water, cooking, cooling to obtain cassava powder liquefied liquid, fermenting the cassava dreg slurry for a period of time, adding saccharifying enzyme and the cassava powder liquefied liquid under the aseptic operation condition, and obtaining ethanol fermented mash by adopting a conventional ethanol fermentation process.
Description
Technical field
The invention belongs to technical field of biochemical industry, are related to a kind of waste residue generated after Anaerobic Treatment by cassava alcohol factory
The method that ethyl alcohol is produced for raw material simultaneous saccharification and fermentation.
Background technology
In recent years, alcohol fuel is increasingly subject to the attention of various countries as new recyclable fuel substitute, wherein non-grain second
Alcohol is the basic orientation of industry development from now on, and as the transition from grain alcohol to cellulosic ethanol, Cassava-based ethanol is current
One of development priority.One of the characteristics of this intermediate item is accordingly to generate a large amount of waste residues, often produces 1 ton of Cassava-based ethanol and about generates
How 0.33 ton of manioc waste (butt), therefore, improve the comprehensive utilization value of manioc waste, turn waste into wealth, and is cassava alcohol industry
Urgent problem to be solved.
At present manioc waste be mainly used for the raw material of Alcohol Production open source literature report it is as follows:
" 1. the Study on Fermentation of manioc waste " (Su little Jian, Xie Lixia etc., Guangxi Normal University's environment and Zi Huan institutes,
《Grain and oil are processed》, 2010,10:Generated waste solid during producing starch using cassava 127-129) is had studied to ferment
The optimal conditions of ethanol production, the results showed that:In material-water ratio 1: 4,35 DEG C of temperature, yeast specie is high activity dried yeast, fiber
The maximum output of ethyl alcohol is 27.3% under conditions of plain enzyme dosage is 0.15g.
" 2. novel technique for producing manioc waste ethanol " (Guangxi Ming Yang biotech inc, patent No.:
CN101195836A separates manioc waste and the raw cassava or tapioca slice after producing starch, carries out boiling and saccharification, wood respectively
Potato slag carries out pulp water separation after saccharification, and solid slag is used to give birth to directly as feed or fertilizer, the manioc waste saccharification filtrate of separation
Cassava or tapioca slice are sized mixing with liquid, further fermentative production of ethanol.After this technique, the tapioca slice consumption of ton ethyl alcohol
2.2 tons are down to by 2.7 tons.But the utilization rate of manioc waste is not high in this method, and therefore, the yield of ethyl alcohol is restricted.
" 3. the method that ethyl alcohol is prepared using the raw material containing manioc waste " (Cofco Group Co., Ltd., patent No.:
CN101423849A refers to mix the raw material containing manioc waste with enzyme, digests the cellulose in the raw material containing manioc waste, obtains
To enzymolysis product, and the enzymolysis product that ferments, wherein, the method that the raw material containing manioc waste is mixed with enzyme includes that manioc waste will be contained
Raw material mixed in batches with enzyme, the number in batches is at least 2 times, and the amount of the raw material containing manioc waste that every batch of adds in is at most complete
The 60% of material quality of the portion containing manioc waste although the method can improve the utilization rate of the raw material containing manioc waste, employs first sugar
The mode fermented after change, utilization rate of equipment and installations is low, and the production cycle is long, while concentration of alcohol is relatively low, enables subsequent product subtractive process
Consumption is higher.
As it can be seen that presently used manioc waste raw material is the waste residue of starch factory mostly, minority is given birth to using cassava alcohol factory waste residue
The technique of producing and ethanol is not there are the problem of the utilization rate of manioc waste is high, and concentration of alcohol is relatively low and utilization rate of equipment and installations is low, with cassava
The development of ethyl alcohol industry, there is an urgent need to find a kind of new process that ethyl alcohol is produced using manioc waste as raw material, to improve cassava fuel
The whole economic efficiency of ethyl alcohol device.
Invention content
The main object of the present invention is that the waste residue generated after anaerobic fermentation for cassava alcohol factory provides a kind of utilization rate height,
The high method of concentration of alcohol in zymotic fluid improves the economic benefit of cassava fuel alcohol plant.
In order to achieve the above objectives, the present invention provides a kind of method of manioc waste fermentative production of ethanol, which is characterized in that including
Following steps:
(1) manioc waste is crushed, water is added to be tuned into manioc waste slurry, pH is adjusted to 4.0~5.0, then add in 0.5g/L~
The ammonium sulfate of 0.75g/L, high pressure steam sterilization, cooling;
(2) the manioc waste slurry in step (1) after sterilizing adds in cellulase, while add in wine under aseptic technique
Wine active dry yeast, first aerobic fermentation 4h~6h, then anaerobic fermentation are added batch-wise manioc waste in the 20h of fermentation, make final cassava
The mass ratio of slag and water is 1:2~1:3;
(3) weigh 3~4 times of the tapioca starch that step (2) adds in manioc waste quality, after adding in alpha-amylase, with water with
1:2~1:2.5 ratio is uniformly mixed, and is put into 90 DEG C~97 DEG C water-baths, the postcooling of boiling 20min~40min obtains wood
Potato powder liquefier;
(4) when manioc waste slurry is fermented to 15h~25h described in step (2), saccharification is added under aseptic technique
Enzyme, while the tapioca starch liquefier that step (3) obtains is added in, then using alcohol fermentation technique, alcohol fermentation wine with dregs is obtained, then pass through
Ethyl alcohol is obtained after distillation.
The method of manioc waste fermentative production of ethanol of the present invention, in step (1), it is preferred that after manioc waste crushes,
Part of the screening grain size less than 0.85mm adds water to be tuned into manioc waste slurry.
The method of manioc waste fermentative production of ethanol of the present invention, in step (1), it is preferred that the cassava slurry
In material, the mass ratio of manioc waste and water is 1:4~1:8, in step (1) and step (2), the manioc waste be it is dry after it is aqueous
Manioc waste of the amount less than 5%.
The method of manioc waste fermentative production of ethanol of the present invention, in step (1), the high pressure steam sterilization it is specific
Condition is preferably:110 DEG C~115 DEG C, pressure 0.14MPa~0.17MPa, sterilize 20min~40min.
The method of manioc waste fermentative production of ethanol of the present invention, in step (1), it is preferred that the cassava after sterilizing
Slurry material is cooled to 30 DEG C~35 DEG C.
The method of manioc waste fermentative production of ethanol of the present invention, in step (2), the additive amount of the cellulase is excellent
It is selected as every gram of 15~50 enzyme activity unit of manioc waste.
The method of manioc waste fermentative production of ethanol of the present invention, in step (2), the Active Dry Yeast adds
Dosage is preferably per litre potato slurry material 0.5g~1.5g.
The method of manioc waste fermentative production of ethanol of the present invention, in step (2), the fermentation temperature of the manioc waste slurry
Preferably 32 DEG C~37 DEG C of degree.
The method of manioc waste fermentative production of ethanol of the present invention, in step (3), the addition of the alpha-amylase is excellent
It is selected as every gram of tapioca starch 8U~15U.
The method of manioc waste fermentative production of ethanol of the present invention, in step (4), the additive amount of the carbohydrase is preferred
For every gram of tapioca starch 150U~200U.
The method of manioc waste fermentative production of ethanol of the present invention, in step (4), the fermentation of the alcohol fermentation technique
Temperature is preferably 32 DEG C~37 DEG C.
The method of manioc waste fermentative production of ethanol of the present invention can obtain the alcohol fermentation of volumetric concentration 14% or so
Wine with dregs.
Invention effect:
(1) the raw materials used in the present invention is manioc waste (alternatively referred to as cassava alcohol slag), is the production ethyl alcohol production of cassava alcohol factory
Raw waste residue, wherein main component are cellulose, hemicellulose, lignin, are substantially free of starch.It is proposed by the present invention with cassava
The method that the waste residue after Anaerobic Treatment that Alcohol Plant generates produces ethyl alcohol for raw material simultaneous saccharification and fermentation, solves manioc waste and gives up
The Utilizing question of gurry improves the whole economic efficiency of Cassava-based ethanol device.
(2) by the way of simultaneous saccharification and fermentation, saccharification and fermentation carry out in same reactor, low equipment investment,
It is with short production cycle, while concentration of glucose can be made to keep relatively low level, be conducive to prevent microbiological contamination.
(3) on the basis of manioc waste fermentative production of ethanol, a kind of manioc waste and tapioca starch mixed raw material fermentation work are proposed
Skill improves ethanol production under the premise of cassava dosage is not increased, meanwhile, ethyl alcohol volumetric concentration reaches 14% left side in zymotic fluid
The right side will not increase the energy consumption of subsequent product subtractive process.It in production can as needed and actual conditions flexibly take independent hair
Ferment or the mode of mixed fermentation
(4) for the technique without being transformed to existing equipment, cassava alcohol slag needs not move through pretreatment, can be direct after crushing
As the raw material of fermentation, energy consumption is greatly reduced, it is simple for process, simple and easy to do, while reduce the discharge capacity of waste cassava residues.
Specific embodiment
The present invention is described further for the following examples, but not thereby limiting the invention.
Manioc waste used in the present embodiment is the waste residue after Anaerobic Treatment that cassava alcohol factory generates, into being grouped as
(mass fraction %) such as following table:
Cellulose | Lignin | Hemicellulose | Ash content | It is other |
31.04 | 14.59 | 12.23 | 21.71 | 20.43 |
Embodiment 1
Manioc waste takes the part 400g (over dry) that grain size is less than 0.85mm after crushed, and water 2000mL is added in 5L fermentation tanks
Slurry is tuned into, then adds in the ammonium sulfate of 1.5g, pH value is adjusted to 4.5, in 110 DEG C of high pressure steam sterilization 20min, is cooled to 30
DEG C, cellulase 3200FPU and 2g Active Dry Yeast is added in, fermentation temperature control is 35 DEG C, in fermentation process in 20h
Batch feeding, making total manioc waste addition, volume fraction of ethanol reached 7.5% at 36 hours in zymotic fluid, ethyl alcohol for 800g
Yield is 0.15g/g.
Embodiment 2
Manioc waste takes the part 400g (over dry) that grain size is less than 0.85mm after crushed, and water 2000mL is added in 5L fermentation tanks
Slurry is tuned into, then adds in the ammonium sulfate of 1.0g, pH value is adjusted to 4.0, in 110 DEG C of high pressure steam sterilization 20min, is cooled to 30
DEG C, cellulase 1200FPU and 1g Active Dry Yeast is added in, fermentation temperature control is 37 DEG C, in fermentation process in 20h
Batch feeding, making total manioc waste addition, volume fraction of ethanol reached 4.9% at 40 hours in zymotic fluid, ethyl alcohol for 1000g
Yield is 0.08g/g.
Embodiment 3
Manioc waste takes the part 250g (over dry) that grain size is less than 0.85mm after crushed, and water 2000mL is added in 5L fermentation tanks
Slurry is tuned into, then adds in the ammonium sulfate of 1.2g, pH value is adjusted to 5.0, in 110 DEG C of high pressure steam sterilization 20min, is cooled to 30
DEG C, cellulase 4000FPU and 3g Active Dry Yeast is added in, fermentation temperature control is 32 DEG C, in fermentation process in 20h
Batch feeding, making total manioc waste addition, volume fraction of ethanol reached 6.3% at 32 hours in zymotic fluid, ethyl alcohol for 667g
Yield is 0.15g/g.
Embodiment 4
(1) manioc waste crushes, and the manioc waste 100g (over dry) that grain size is taken to be less than 0.85mm adds water 600ml in triangular flask
Slurry is tuned into, then adds in the ammonium sulfate of 0.45g, pH value is adjusted to 4.5, in 110 DEG C of high pressure steam sterilization 20min, is cooled to 30
℃;
(2)) above-mentioned manioc waste slurry adds in cellulase (outstanding promise biology Enzyme Co., Ltd) 600FPU and 0.6g wine brewing and lives
Property dry ferment, shaking table revolution 150r/min, fermentation temperature is 35 DEG C, is ensureing that zymotic fluid has stream in 20h in fermentation process
Batch feeding under the premise of dynamic property, it is 200g to make total manioc waste addition;
(3) tapioca starch 600g is weighed, adds in alpha-amylase 6000U, in 5L fermentation tanks plus water 1200ml is uniformly mixed,
Under the conditions of 100 DEG C 30 DEG C are cooled to after boiling 30min;
(4) when the manioc waste of step (2) is fermented to 20h, the 5L fermentation tanks of step (3) are added under aseptic technique
In, while carbohydrase is added in, the additive amount of carbohydrase is 108000U;Under the conditions of fermentation temperature is 35 DEG C, using conventional ethanol
Zymotechnique, volume fraction of ethanol reached 14% at 48 hours in zymotic fluid, obtained alcohol fermentation wine with dregs.
Comparative example 1
(1) tapioca starch 600g is weighed, adds in the ammonium sulfate of alpha-amylase 6000U and 0.45g, water is added in 5L fermentation tanks
1800mL is uniformly mixed;30 DEG C are cooled to after boiling 30min under the conditions of 100 DEG C;
(2) manioc waste takes the part 200g (over dry) that grain size is less than 0.85mm after crushed, in 110 DEG C of high pressure steam sterilizations
It is added in the 5L fermentation tanks of step (1) after 20min, while adds in cellulase 600FPU and 1.8g Active Dry Yeast, sent out
The control of ferment temperature is 35 DEG C, and volume fraction of ethanol reached 13.5% at 48 hours in zymotic fluid.
Comparative example 2
(1) tapioca starch 600g is weighed, adds in alpha-amylase 6000U, in 5L fermentation tanks plus water 1800ml is uniformly mixed;
Under the conditions of 100 DEG C 30 DEG C are cooled to after boiling 30min;
(2) 1.8g Active Dry Yeasts are added in, while add in carbohydrase, the additive amount of carbohydrase is 108000U;
(3) under the conditions of fermentation temperature is 35 DEG C, using conventional ethanol zymotechnique, volume fraction of ethanol exists in zymotic fluid
Reach 12.8% within 48 hours.
Certainly, the present invention can also have other various embodiments, without deviating from the spirit and substance of the present invention, ripe
Various corresponding changes and deformation, but these corresponding changes and deformation can be made according to the present invention by knowing those skilled in the art
It should all belong to the scope of protection of the present invention.
Claims (11)
- A kind of 1. method of manioc waste fermentative production of ethanol, which is characterized in that include the following steps:(1) manioc waste is crushed, water is added to be tuned into manioc waste slurry, pH is adjusted to 4.0~5.0, then adds in 0.5g/L~0.75g/L Ammonium sulfate, high pressure steam sterilization, cooling;(2) the manioc waste slurry in step (1) after sterilizing adds in cellulase under aseptic technique, while adds in wine brewing and live Property dry ferment, manioc waste is added batch-wise in first aerobic fermentation 4h~6h, then anaerobic fermentation, the 20h of fermentation, make final manioc waste with The mass ratio of water is 1:2~1:3;(3) 3~4 times of the tapioca starch that step (2) adds in manioc waste quality is weighed, after adding in alpha-amylase, with water with 1:2~ 1:2.5 ratio is uniformly mixed, and is put into 90 DEG C~97 DEG C water-baths, the postcooling of boiling 20min~40min obtains tapioca starch Liquefier;(4) when manioc waste slurry is fermented to 15h~25h described in step (2), carbohydrase is added under aseptic technique, together When add in step (3) obtained tapioca starch liquefier, then using alcohol fermentation technique, obtain alcohol fermentation wine with dregs, then through distillation After obtain ethyl alcohol.
- 2. the method for manioc waste fermentative production of ethanol according to claim 1, which is characterized in that in step (1), manioc waste After crushing, part of the screening grain size less than 0.85mm adds water to be tuned into manioc waste slurry.
- 3. the method for manioc waste fermentative production of ethanol according to claim 1, which is characterized in that in step (1), the wood In potato slurry material, the mass ratio of manioc waste and water is 1:4~1:8, in step (1) and step (2), the manioc waste is drying Water content is less than 5% manioc waste afterwards.
- 4. the method for manioc waste fermentative production of ethanol according to claim 1, which is characterized in that in step (1), the height Pressure steam sterilizing actual conditions be:110 DEG C~115 DEG C, pressure 0.14MPa~0.17MPa, sterilize 20min~40min.
- 5. the method for manioc waste fermentative production of ethanol according to claim 1, which is characterized in that in step (1), after sterilizing Manioc waste slurry, be cooled to 30 DEG C~35 DEG C.
- 6. the method for manioc waste fermentative production of ethanol according to claim 1, which is characterized in that in step (2), the fibre The additive amount of the plain enzyme of dimension is every gram of 15~50 enzyme activity unit of manioc waste.
- 7. the method for manioc waste fermentative production of ethanol according to claim 1, which is characterized in that in step (2), the wine The additive amount of wine active dry yeast is per litre potato slurry material 0.5g~1.5g.
- 8. the method for manioc waste fermentative production of ethanol according to claim 1, which is characterized in that in step (2), the wood The fermentation temperature of potato slurry material is 32 DEG C~37 DEG C.
- 9. the method for manioc waste fermentative production of ethanol according to claim 1, which is characterized in that in step (3), the α- The addition of amylase is every gram of tapioca starch 8U~15U.
- 10. the method for manioc waste fermentative production of ethanol according to claim 1, which is characterized in that described in step (4) The additive amount of carbohydrase is every gram of tapioca starch 150U~200U.
- 11. the method for manioc waste fermentative production of ethanol according to claim 1, which is characterized in that described in step (4) The fermentation temperature of alcohol fermentation technique is 32 DEG C~37 DEG C.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110564780A (en) * | 2019-09-16 | 2019-12-13 | 华南农业大学 | Method for co-producing biogas by fermenting ethanol with bagasse-molasses-yam slag mixed raw material |
CN112708640A (en) * | 2021-01-21 | 2021-04-27 | 广东莞垄农业生态科技有限公司 | Method for extracting alcohol by processing and fermenting cassava extracted starch |
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