CN108226481A - A kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent - Google Patents
A kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent Download PDFInfo
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- CN108226481A CN108226481A CN201810013947.4A CN201810013947A CN108226481A CN 108226481 A CN108226481 A CN 108226481A CN 201810013947 A CN201810013947 A CN 201810013947A CN 108226481 A CN108226481 A CN 108226481A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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Abstract
The present invention relates to a kind of magnetic bead reagents in chemiluminescence immunoassay detection reagent.The magnetic bead reagent is by immunomagnetic beads, base soln and thickener composition, the physical aspect of the reagent has temperature dependency, i.e. under the conditions of the reagent certain temperature jelly or solid forms, reagent mobility is extremely low, with its physical aspect of temperature rise is write as liquid is presented, restores mobility, immunomagnetic beads are dispersed in reagent, not free settling.In reagent jelly or solid forms, can effectively avoid the immunomagnetic beads caused by the cold chain transportation process be adhered to packaging film or tube wall caused by loss, so as to ensure the accuracy of detection；When non-liquid is presented due to temperature change in physical aspect, immunomagnetic beads are dispersed in reagent, not free settling.During packing, it is ensured that the consistency of magnetic bead component agent.The present invention is suitable for electrochemiluminescent immunoassay detection kit, especially examines (POCT) soon with kit or reagent strip.
Description
Technical field
The present invention relates to a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent, which is suitable for chemistry
Luminescent immunoassay reagent, belongs to pharmaceutical technology field.
Background technology
Immunomagnetic beads are usually by small metal particles (Fe2O3Or Fe3O4) core and the macromolecule material containing specified chemical group
Material composition.Immunomagnetic beads have superparamagnetism, can be dispersed in solution, functional group such as amino, carboxyl, the mercapto on surface
Base etc. can be with antibody covalently or non-covalent associations, then with reference to corresponding antigen or antibody, outside plus can be fast under magnetic fields
Speed absorption, reaches the purpose of purified genes, protein, cell, microorganism (Borlido L, Azevedo A M, Roque A C
A,et al.Magnetic separations in biotechnology[J].Biotechnology advances,2013,
31(8):1374-1385).In addition, immunomagnetic beads have good skin effect, large specific surface area, functional group and selectivity
Adsorption capacity is big, increases substantially reagent sensitivity.
Immunomagnetic beads are widely used in chemiluminescence immune assay (Yang H, Liang as solid phase carrier at present
W,He N,et al.Chemiluminescent labels released from long spacer arm-
functionalized magnetic particles:a novel strategy for ultrasensitive and
highly selective detection of pathogen infections[J].ACS applied materials&
interfaces,2014,7(1):774-781；Iranifam M.Analytical applications of
chemiluminescence-detection systems assisted by magnetic microparticles and
nanoparticles[J].TrAC Trends in Analytical Chemistry,2013,51:51-70).Due to immune
Bead density is much larger than water, often easily occurs precipitating and vessel surface attachment phenomenon in water phase preserves reagent.In immunoassay
In the process, if immunomagnetic beads disperse unevenness in preservation system, it is most likely that subsequent measurements result is caused to generate obvious deviation,
In it is especially the most apparent to detect immediately on (point-of-care testing, POCT) reagent card.
Invention content
Present invention aim to address above-mentioned problems of the prior art, provide a kind of magnetic bead at a lower temperature and try
Gluey or solid forms are presented in agent, and the magnetic bead reagent of liquid form is presented after temperature raising.Under glue/solid forms,
Immunomagnetic beads particle is in homogeneously dispersed state and is not easy to precipitate, while the temperature dependency of reagent physical aspect is largely kept away
Exempted from cold chain transportation process because concussion or reagent be inverted caused by immunomagnetic beads be adhered in tube wall or sealer the problem of.And once
In detection in use, the physical aspect of immunomagnetic beads reagent is rendered as liquid, immune analysis determination is not influenced as a result, particularly suitable
In especially chemiluminescent quick detection (POCT) kit of chemiluminescence detection kit or reagent card.
The technical solution adopted by the present invention is：
1. a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent of the present invention, it is characterised in that the reagent is
It is made of immunomagnetic beads, base soln and thickener；The physical aspect of the reagent has temperature dependency, i.e. one constant temperature of reagent
Under the conditions of degree jelly or solid forms, reagent mobility is extremely low, with writing the physical aspect of temperature rise reagent as liquid is presented,
Restore mobility, immunomagnetic beads are dispersed in reagent, not free settling.
2. a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent of the present invention, it is characterised in that the reagent
Physical aspect refers to temperature dependency：
Reagent is placed under the conditions of 2~8 DEG C jelly or reagent form is rendered as liquid during solid forms, 8 DEG C of temperature ＞ or more
Volume morphing；
Reagent is placed under the conditions of≤25 DEG C jelly or solid forms, and reagent form is rendered as liquid shape during 25 DEG C of temperature ＞
State；
Reagent is placed under the conditions of≤37 DEG C jelly or solid forms, and reagent form is rendered as liquid shape during 37 DEG C of temperature ＞
State.
A kind of 3. magnetic bead reagent for chemiluminescence immunoassay detection reagent described in claim 1, it is characterised in that basis
Solution includes buffer solution, inorganic salts and preservative.
4. a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent of the present invention, it is characterised in that in the reagent
Basal liquid include：Buffer solution, inorganic salts, preservative；
Wherein：
Buffer solution is anion buffer, cationic buffer liquid, Good 'sOne kind in buffer solution；
Inorganic salts are one kind in soluble sodium salt or sylvite；
Preservative is one kind in Proclin series or Sodium azide.
5. a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent of the present invention, it is characterised in that in the reagent
Thickener include：Amino acid and protide, carbohydrate, polyalcohol, high molecular polymer and nonionic surface active agent；
Wherein：
The amino acid and protide are glycine, arginine, glutamic acid, alanine, bovine serum albumin(BSA), casein,
One or more mixing in gelatin, gel；
One or more mixing of the carbohydrate for monosaccharide or oligosaccharide；
The polyalcohol is glycerine, one or more mixing in mannitol, D-sorbite, xylitol；
The high molecular polymer is polyethylene glycol (PEG) series (relative molecular mass is 6000~60000) or poly- second
One or more mixing in alkene pyrrolidone (PVP) series (relative molecular mass is 10000~360000)；
The nonionic surface active agent leads to for tween (Tween) series or Qula in (Triton) series or NP-40
One or more mixtures.
6. a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent of the present invention, it is characterised in that therein to exempt from
Epidemic disease magnetic bead is by immune labeled magnetic bead, and marker includes antibody, antigen, BSA, chemistry and biological micromolecule, nucleic acid, fluorescence
Element etc.；Labeling method includes Streptavidin-biotin method or EDC-NHS or other practical methods etc..
7. a kind of magnetic bead reagent of the present invention, it is characterised in that the reagent is made by following steps：Weigh each group
Point, pure water dissolving is added in, mixing filters, and is magnetic bead reagent of the present invention after addition immunomagnetic beads.
8. the application of a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent of the present invention, it is characterised in that should
Magnetic bead reagent is mainly used on clinical immunization fast diagnosis reagent strip, detects strip as chemo-immunity shines, enzyme linked immunological inspection
Survey strip etc..
The specific embodiment of the invention
1. the preparation of the magnetic bead reagent for chemiluminescence immunoassay detection reagent
(1) labeling method of magnetic bead
The immunomagnetic beads are by immune labeled magnetic bead, and marker includes antibody, antigen, BSA, chemistry and biology
Small molecule, nucleic acid, fluorescein etc..Labeling method includes Streptavidin-biotin method or EDC-NHS methods or other practical sides
Method etc..
1) Streptavidin-biotin method：By biotin labeling on antibody, marked by streptavidin in band carboxyl (or
Amino etc.) functional group magnetic bead particles (Streptavidin MagneSphere that commercialization can also be used) on；Then using biotin and
Antibody (or the albumen such as antigen, BSA) is fixed on magnetic bead particles surface by the high affinity between Streptavidin, that is, obtains this
Invent the immunomagnetic beads.
2) EDC-NHS methods：Under NHS (HOSu NHS) existence condition, EDC (carbodiimides) and magnetic bead particles
The carboxyl on surface reacts to form active succinimidyl ester, then with antibody (or antigen, BSA, chemistry and biological micromolecule, nucleic acid,
Fluorescein etc.) on amino react to form stable amido bond, so as to by antibody (or antigen, BSA, chemistry and biological micromolecule,
Nucleic acid, fluorescein etc.) be fixed on magnetic bead particles surface, that is, obtain immunomagnetic beads of the present invention.
(2) preparation of basal liquid
Base soln includes buffer solution, inorganic salts and preservative.Wherein：
Buffer solution is anion buffer, one kind in cationic buffer liquid, Good ' s buffer solutions, such as trihydroxy methyl amino
Methane (Tris), 4- hydroxyethyl piperazineethanesulfonic acids (HEPES), 3- N-morpholinyls (MOPS) or phosphate buffer (PBS).It is excellent
Tris buffer solutions, pH ranging from 4.0~11.0 are selected, buffer concentration is 10~200mM；
Inorganic salts are one kind in soluble sodium salt or sylvite, such as NaCl or KCl, preferably NaCl, concentration range 0.6%~
1.8%；
Preservative for Proclin series or Sodium azide in one kind, wherein Proclin series include Proclin 100,
Proclin200, Proclin 300 or Proclin 950, preferred preservative a concentration of 0.01%~0.1%.
(3) preparation of thickener
Amino acid and albumen, carbohydrate, polyalcohol, high molecular polymer and nonionic surface active agent are any one or more
Mixing is respectively provided with thickener effect.
Wherein amino acid and albumen are glycine, arginine, glutamic acid, alanine, bovine serum albumin(BSA) (BSA), junket egg
In vain, one or more mixtures in gelatin, gel, preferred concentration are 0.05%~5%.
The one kind of carbohydrate for monosaccharide or oligosaccharide, as glucose, galactolipin, sucrose, maltose, glucan, trehalose or
Chitosan etc., preferred concentration are 0.01%~10%.
Polyalcohol is glycerine, one kind in mannitol, D-sorbite, xylitol, and preferred concentration is 0.1%~10%.
High molecular polymer is PEG series (relative molecular mass is 6000~60000) or serial (the average molecular matter of PVP
Measure 10000~360000) in one kind, as PEG 6000, PEG 8000, PEG 20000, PEG 60000, PVP K15,
PVP K30, PVP K60 or PVP K90 etc., preferred concentration are 0.1%~10%.
Nonionic surface active agent is one or more mixtures in Tween series or Triton series or NP-40,
Wherein Tween series including Tween-20, Tween-40 or Tween-80 etc., Triton series include Triton X-100,
Triton X-114, Triton X-405 etc., preferred concentration are 0.01%~1%；
(4) preparation of magnetic bead reagent
Above each ingredient is prepared by formula as below：0.2~1.0mg/mL of immunomagnetic beads, basal liquid (Tris buffer solutions 10~
200mmol/L, preservative 0.01%~1%, NaCl 0.6%~1.8%), thickener 0.01%~10%.By more than each group
Divide and mix and adjust to debita spissitudo, pH is adjusted to 7.5.
2. the application of the magnetic bead reagent of the present invention for chemiluminescence immunoassay detection reagent
Magnetic bead reagent of the present invention is mainly used on clinical immunization fast diagnosis reagent strip, as chemo-immunity shines
Detect strip, enzyme linked immunosorbent detection strip etc..
The positive effect of the present invention
The present invention relates to a kind of magnetic bead reagents in chemiluminescence immunoassay detection reagent.The magnetic bead reagent is by being immunized magnetic
Pearl, base soln and thickener composition, the physical aspect of the reagent has temperature dependency, i.e., under the conditions of the reagent certain temperature
Jelly or solid forms, reagent mobility is extremely low, with its physical aspect of temperature rise is write as liquid is presented, restores mobility,
Immunomagnetic beads are dispersed in reagent, not free settling.In reagent jelly or solid forms, it can effectively avoid because of cold chain transportation
Immunomagnetic beads caused by process are adhered to loss caused by packaging film or tube wall, so as to ensure the accuracy of detection；Physical aspect
When non-liquid is presented due to temperature change, immunomagnetic beads are dispersed in reagent, not free settling.During packing, it can protect
Demonstrate,prove the consistency of magnetic bead component agent.The present invention is suitable for electrochemiluminescent immunoassay detection kit, and especially reagent is used in inspection (POCT) soon
Box or reagent strip.
Embodiment
To further description be made to the present invention by specific embodiment below.The embodiment only makes the present invention
Exemplary illustration not makes the present invention restriction in all senses.
Embodiment 1
--- the preparation of magnetic bead reagent
Embodiment 2
--- different thickening agent combination formulas
By the magnetic bead preparation of reagents method of embodiment 1, different thickening agent combinations and dosage (being shown in Table 1).
Different thickening agent combinations and dosage in 1 magnetic bead reagent of table
Embodiment 3
--- the temperature dependency of different formulations magnetic bead reagent corresponding physical aspect under condition of different temperatures.
Magnetic bead reagent, the physical aspect temperature dependency of identification various combination formula magnetic bead reagent are prepared by Examples 1 to 2
(table 2).
The form of 2 magnetic bead reagent of table at different temperatures
| Group | 2-8℃ | 8-25℃ | 25-36℃ | 37 DEG C and more than |
| Embodiment 2 (1) | It is gluey | Liquid | Liquid | Liquid |
| Embodiment 2 (2) | Solid | It is gluey | Liquid | Liquid |
| Embodiment 2 (3) | Solid | Solid | Liquid | Liquid |
| Embodiment 2 (4) | It is gluey | It is gluey | Liquid | Liquid |
| Embodiment 2 (5) | Solid | It is gluey | Liquid | Liquid |
| Embodiment 2 (6) | Solid | Solid | Solid | Liquid |
| Embodiment 2 (7) | Solid | Liquid | Liquid | Liquid |
| Embodiment 2 (8) | Solid | Solid | Liquid | Liquid |
| Embodiment 2 (9) | Solid | Solid | Solid | Liquid |
Conclusion：Meet the physical aspect temperature dependency of magnetic bead reagent of the present invention according to the result of table 2, i.e., less than
Under the conditions of equal to 25 DEG C (especially 2-8 DEG C) jelly or solid forms.
Embodiment 4
--- magnetic bead reagent of the present invention immune analysis determination step in chemiluminescence POCT is as follows：
Detect preparation routine：By magnetic bead reagent of the present invention, enzyme label antibody reagent, cleaning solution and substrate reagent, to be measured
Sample is filled respectively in the corresponding hole site of measure reagent card；
Start detection program：Taking quantitative sample respectively, enzyme labelled antibody adds in magnetic bead reagent wells position mixing of the present invention, and 37
DEG C be incubated 5 to 10min, collect reaction after immunomagnetic beads particle；Quantitative cleaning solution mixing is separately added into, collects immunomagnetic beads
Grain (repeats 2~3 times), and cleaned immunomagnetic beads particle with substrate reagent is suspended, measures its luminous signal value.
Data processing：According to the luminous signal value obtained and concentration of specimens relationship is measured, sample to be tested concentration value is calculated.
Embodiment 5
--- the test of magnetic bead reagent of the present invention
Magnetic bead reagent is prepared as experimental group by the 7th group of formula in embodiment 1 and embodiment 2, is separately tried using common magnetic bead
Agent is as a control group.Reagent strip packing and test sample are carried out as described in embodiment 4.Measure items selection cardiac muscle troponin I
(cTnI) 3 be the results are shown in Table.
3 cardiac muscle troponin I of table (cTnI) test result
The magnetic bead reagent and common magnetic bead that the 6th group of formula of embodiment 2 is prepared two projects measure large deviations 5% with
It is interior, illustrate that magnetic bead reagent test accuracy of the present invention is good, chemiluminescence immunoassay will not be interfered to react.
Embodiment 6
--- magnetic bead reagent of the present invention is applied to the performance evaluation of chemiluminescence immunoassay：
Take marked by streptavidin magnetic bead (JSR, article No.：MS150/Streptavidin), with MES buffer solution for cleaning 3 times
It is coupled under room temperature with biotin antibody afterwards.After reaction, Excess antibody is washed away.Then by the 3rd group of formula in embodiment 2
Magnetic bead reagent is prepared as experimental group, separately using common magnetic bead reagent as a control group.Reagent strip point is carried out as described in embodiment 4
Dress and test sample.Measure items selection cardiac muscle troponin I (cTnI) and creatine kinase isozyme (CK-MB), each project
Each 10 samples, precision evaluation are tried using 2 batches immunomagnetic beads reagent of the present invention and the common magnetic bead of a batch
Agent, evaluation result such as table 4, shown in table 5：
4 magnetic bead reagent accuracy estimating result of the present invention of table
The magnetic bead reagent and common magnetic bead that the 2nd group of formula of embodiment 2 is prepared two projects measure large deviations 5% with
It is interior, illustrate that magnetic bead reagent test accuracy of the present invention is good, chemiluminescence immunoassay will not be interfered to react.
5 magnetic bead reagent accurate degree evaluation result of the present invention of table
Magnetic bead reagent variation within batch coefficient of the present invention<5%, interassay coefficient of variation<5%, illustrate magnetic bead examination of the present invention
Agent precision is good.
Embodiment 7
--- magnetic bead reagent of the present invention and common magnetic bead reagent simulation transport contrast experiment
Take marked by streptavidin magnetic bead (JSR, article No.：MS150/Streptavidin), with MES buffer solution for cleaning 3 times
It is coupled under room temperature with biotin antibody afterwards.After reaction, Excess antibody is washed away.Then by the 3rd group of formula in embodiment 2
Prepare magnetic bead reagent as experimental group, separately using common magnetic bead reagent as a control group.Reagent strip point is carried out as described in embodiment 3
Dress.Reagent strip equipped with 2 kinds of magnetic bead reagents is respectively divided into 2 groups, group number A and B, A group is set (to keep temperature using cryogenic box respectively
2~25 DEG C of degree) transportational process is simulated, B groups are placed in 2~8 DEG C of preservations.Sample measures, the project of measure are carried out as described in embodiment 3
Cardiac muscle troponin I (cTnI) is selected, each each 10 samples of project, the results are shown in Table 6.
6 magnetic bead reagent of the present invention of table and common magnetic bead reagent transport simulation inspection result
As a result and the control group deviation that keeps sample magnetic bead reagent of the present invention carries out test sample after transport simulation,<5%,
And test result after common magnetic bead transport simulation and keep sample control group deviation mostly>5%, close to 10%.Therefore, magnetic of the present invention
Pearl advantage under traffic condition is notable.
Embodiment 8
--- the accuracy estimating of EDC-NHS methods label magnetic bead reagent of the present invention
Take carboxyl magnetic bead (JSR, article No.：MX100/Carboxyl), with MES buffer solution for cleaning 3 times.It is activated with EDC and NHS
Group on magnetic bead adds in antibody.3h is marked under the conditions of 2-8 DEG C.After terminating reaction, with MES buffer solution for cleaning 3 times.Then it presses
The 3rd group of formula prepares magnetic bead reagent as experimental group in embodiment 2, separately using common magnetic bead reagent as a control group.By implementation
Reagent strip packing and test sample are carried out described in example 3.The items selection cardiac muscle troponin I (cTnI) and creatine kinase of measure are same
Work enzyme (CK-MB), each each 10 samples of project, the results are shown in Table 7.
7 magnetic bead reagent accuracy estimating result of the present invention of table
Magnetic bead reagent of the present invention with EDC/NHS labels is compared with common magnetic bead reagent, in two projects measure
Deviation illustrates that magnetic bead reagent test accuracy of the present invention is good, chemiluminescence immunoassay will not be interfered to react within 5%.
Claims (10)
1. a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent, it is characterised in that the reagent is by immunomagnetic beads, base
Plinth solution and thickener composition；The physical aspect of the reagent has temperature dependency, i.e., is in glue under the conditions of the reagent certain temperature
Shape or solid forms, reagent mobility is extremely low, is presentation liquid with the physical aspect of work temperature rise reagent, restores mobility,
Immunomagnetic beads are dispersed in reagent, not free settling.
A kind of 2. magnetic bead reagent for chemiluminescence immunoassay detection reagent as described in claim 1, it is characterised in that the reagent
Physical aspect refer to temperature dependency：
Reagent is placed under the conditions of 2~8 DEG C jelly or reagent form is rendered as liquid shape during solid forms, 8 DEG C of temperature ＞ or more
State；
Reagent is placed under the conditions of≤25 DEG C jelly or solid forms, and reagent form is rendered as liquid form during 25 DEG C of temperature ＞；
Reagent is placed under the conditions of≤37 DEG C jelly or solid forms, and reagent form is rendered as liquid form during 37 DEG C of temperature ＞.
A kind of 3. magnetic bead reagent for chemiluminescence immunoassay detection reagent described in claim 1, it is characterised in that base soln
Comprising buffer solution, inorganic salts and preservative.
A kind of 4. magnetic bead reagent for chemiluminescence immunoassay detection reagent as described in claim 1, it is characterised in that the reagent
In：
Buffer solution is anion buffer, one kind in cationic buffer liquid, Good ' s buffer solutions；
Inorganic salts are one kind in soluble sodium salt or sylvite；
Preservative is one kind in Proclin series or Sodium azide.
A kind of 5. magnetic bead reagent for chemiluminescence immunoassay detection reagent as described in claim 1, it is characterised in that the reagent
In thickener include：Amino acid and protide, carbohydrate, polyalcohol, high molecular polymer and nonionic surface active agent；
Wherein：
The amino acid and protide are glycine, arginine, glutamic acid, alanine, bovine serum albumin(BSA), casein, gelatin,
One or more mixing in collagen；
One or more mixing of the carbohydrate for monosaccharide or oligosaccharide；
The polyalcohol is glycerine, one or more mixing in mannitol, D-sorbite, xylitol；
The high molecular polymer is polyethylene glycol (PEG) series (relative molecular mass is 6000~60000) or polyethylene pyrrole
One or more mixing in pyrrolidone (PVP) series (relative molecular mass is 10000~360000)；
The nonionic surface active agent for tween (Tween) series or Qula lead to (Triton) series or NP-40 in one
Kind or a variety of mixtures.
6. the magnetic bead reagent described in claim 1 for chemiluminescence immunoassay detection reagent, it is characterised in that the reagent by
Following steps are made：Each component is weighed, adds in pure water dissolving, mixing filters, and is magnetic of the present invention after addition immunomagnetic beads
Pearl reagent.
7. a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent as described in claim 1, it is characterised in that therein
Immunomagnetic beads are by immune labeled magnetic bead.
8. a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent as described in claim 1, it is characterised in that wherein exempt from
The marker of epidemic disease label magnetic bead includes antibody or antigen or BSA or chemical small molecule or biological micromolecule or nucleic acid or fluorescein.
9. a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent as described in claim 1, it is characterised in that wherein exempt from
The labeling method of epidemic disease label magnetic bead includes Streptavidin-biotin method or EDC-NHS or other practical methods.
10. the application of a kind of magnetic bead reagent for chemiluminescence immunoassay detection reagent described in claim 1, it is characterised in that main
Clinical immunization fast diagnosis reagent strip is used for, strip, enzyme linked immunosorbent detection strip etc. is detected as chemo-immunity shines.
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| CN109725148A (en) * | 2018-12-30 | 2019-05-07 | 广东环凯微生物科技有限公司 | A kind of immunomagnetic beads preservation liquid |
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| CN110609134A (en) * | 2019-10-14 | 2019-12-24 | 珠海丽珠试剂股份有限公司 | Magnetic particle preservation solution, preparation method and use method thereof, immunoreaction reagent and application thereof |
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| CN111879930A (en) * | 2020-08-04 | 2020-11-03 | 上海艾瑞德生物科技有限公司 | Creatine kinase isoenzyme MB detection kit and preparation method thereof |
| CN114384239A (en) * | 2021-12-09 | 2022-04-22 | 深圳君和生物科技有限公司 | Preservation system of immunomagnetic beads and immunomagnetic bead system |
| CN114397443A (en) * | 2021-12-09 | 2022-04-26 | 华南理工大学 | Preservation solution for immunomagnetic beads and reagents for immunomagnetic beads |
| CN114539787A (en) * | 2022-01-17 | 2022-05-27 | 杭州睿丽科技有限公司 | Magnetic bead reagent stabilizer for stable transportation and preparation method thereof |
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