CN108120666A - For the Optical devices of blood cell analysis - Google Patents
For the Optical devices of blood cell analysis Download PDFInfo
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- CN108120666A CN108120666A CN201711087648.7A CN201711087648A CN108120666A CN 108120666 A CN108120666 A CN 108120666A CN 201711087648 A CN201711087648 A CN 201711087648A CN 108120666 A CN108120666 A CN 108120666A
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- 230000003287 optical effect Effects 0.000 title claims abstract description 50
- 210000000601 blood cell Anatomy 0.000 title claims abstract description 27
- 238000004458 analytical method Methods 0.000 title claims abstract description 26
- 241001270131 Agaricus moelleri Species 0.000 claims abstract description 17
- 230000005540 biological transmission Effects 0.000 claims description 36
- 239000000758 substrate Substances 0.000 claims description 7
- 230000005622 photoelectricity Effects 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 238000009738 saturating Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 15
- 238000000034 method Methods 0.000 description 11
- 238000001514 detection method Methods 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000010276 construction Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000005286 illumination Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 238000004820 blood count Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000009699 differential effect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004148 unit process Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1493—Particle size
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The present invention provides a kind of Optical devices for blood cell analysis, which includes being arranged in order the laser assembly of setting, sample rectifier stack and scattering light-receiving component.Laser assembly is for the irradiation sample rectifier stack that emits beam, and scattering light-receiving component is for receiving from sample rectifier stack transmitted through the light come.Laser assembly includes laser, flat-top optical element and focus lamp, and laser is for emitting beam, and focus lamp is oppositely arranged with laser, and flat-top optical element is arranged between laser and focus lamp.For focus lamp for being focused to light, the light that flat-top optical element is used to send in laser is converted into the equally distributed light of energy.It applies the technical scheme of the present invention, improves to the resolution capability of haemocyte and cluster property, requirement of the Optical devices to fluid path in blood cell analysis can also be reduced, reduce complete machine cost.
Description
Technical field
The present invention relates to technical field of optical detection, are filled in particular to a kind of optics for blood cell analysis
It puts.
Background technology
Blood routine detection is the vitro detection project that situation of all-level hospitals is carried out extensively, at this stage to the side of blood count classification
Method generally use impedance method and optical method.Impedance method according to size of blood cells due to simply carrying out classification differentiation, to leucocyte
Can only obtain three points of heap sorts as a result, on differential hematology analyzer impedance method largely be applied to red blood cell and blood it is small
Plate counts, and optical method is comparatively using more extensively.Due to being to be classified using haemocyte multidimensional information to cell,
Therefore haemocyte, blood platelet and leucocyte can not only be counted, moreover it is possible to leucocyte is carried out finer and is divided, and
Special cells can be distinguished, such as granulophilocyte, abnormal lymphocytes and immature cell, medicine just before giving birth meaning is more
Greatly.
Five classification cellanalyzers are a kind of detections for carrying out quantitative analysis and sorting to haemocyte on functional level
Means, can be with the thousands of a cells of high speed analysis, and can measure multiple parameters from a cell simultaneously, big such as cell
Small, active, granularity, quantity of nucleic acid etc..Its testing principle is：Laser beam is irradiated to unicellular sample flow after shaping
On, the cell for passing sequentially through detection zone generates scattering light or fluorescence under the irradiation of laser beam, then by scattering light or fluorescence
It collects as much as possible collect of camera lens and scatters light or fluorescence signal, then can pass through dichroic mirror and scattering light and fluorescence are carried out by ripple
It is long to separate, then converge to that photodetector collects scattering light or fluorescence signal, different scattering light or fluorescence signal are combined into two
Scatter diagram is tieed up, so as to fulfill the classification quantitative analysis to haemocyte.
In high-end differential hematology analyzer, most manufacturers are all that (forward scattering is dissipated with lateral using light is scattered
Penetrate), transmitted light, the light sources such as fluorescence or polarised light are combined haemocyte to be identified differentiation.Matched optics
System, reagent system, liquid channel system and hardware system are all very complicated, manufacture cost, equipment cost and use cost also very
Height, this kind equipment are commonly available to large hospital more than front three.
For in the differential hematology analyzer of low and middle-end, being essentially all that leucocyte is carried out using forward scattering light
Classification, according to small angle scattering light reaction cell size, wide-angle forward scattering reacting cells inside complexity.In this species five
In classification blood analyzer, it will usually which, using multiple aspherical mirrors, cylindrical mirror and Amici prism, manufacture cost is also higher.
Above-mentioned five classification cellanalyzers lighting source is using the direct light extraction of semiconductor laser, through non-spherical lens
Into vertical strip hot spot after collimation, then by two panels crossed cylindrical mirror both horizontally and vertically to strip collimation laser light
Spot is compressed, and so as to obtain illuminating the Gaussian spot of the horizontal ellipse shape of haemocyte, the spot size is usually about 220
± 20 × 20 ± 10um (13.5%) left and right.
Using aforesaid way, to form more uniform illumination spot in cell stream, only allow the centre of ellipse light spot
Partial illumination is in cell liquid stream.And use fluid focus liquid channel system it is difficult to ensure that in certain sample flow width cell sample
This is distributed in the energy maximum position at Gaussian Profile elliptical spot center still in center, thus the efficiency of light energy utilization compared with
It is low.And because the energy of hot spot can cause the scattering light CV that cell is excited in the different position of sample flow to become in Gaussian Profile
Change, so as to cause the similar haemocyte dispersion of distribution big, different haemocyte class spacing narrow, and blood cell differential effect is poor.It is high simultaneously
This spot side-lobe can cause blood count to identify mistake, affect the Measurement Resolution of cellanalyzer as bias light.
The content of the invention
It is a primary object of the present invention to provide a kind of Optical devices for blood cell analysis, to solve the prior art
In for blood cell analysis Optical devices five classification blood cell analysis in analytical effect it is poor the problem of.
To achieve these goals, the present invention provides a kind of Optical devices for blood cell analysis, including successively
Laser assembly, sample rectifier stack and the scattering light-receiving component of spread configuration, laser assembly is for the irradiation that emits beam
Sample rectifier stack, scattering light-receiving component is for receiving from sample rectifier stack transmitted through the light come；Laser assembly bag
It includes：Laser, for emitting beam；Focus lamp is oppositely arranged with laser, for being focused to light；Flat-top optical element,
It is arranged between laser and focus lamp, it is equally distributed that the light that flat-top optical element is used to send in laser is converted into energy
Light.
Further, laser assembly further includes collimation lens, collimation lens be arranged on flat-top optical element and laser it
Between, collimation lens is used to collimate the light that laser is sent.
Further, collimation lens is aspheric collimation lens.
Further, the numerical aperture of collimation lens is greater than or equal to 0.5.
Further, the focal length of focus lamp is 40mm~60mm.
Further, scattering light-receiving component includes：Fixing bracket；Diaphragm, on fixing bracket, and it is whole with sample
It is adjoining to flow component；Photoelectronic detecting array part, peace turn in fixing bracket, and mutually separate compared with diaphragm and sample rectifier stack.
Further, there are two being formed on diaphragm transmitance region is scattered along the semicircular low angle of center line symmetrical setting
Domain and formation are same positioned at center line there are two the semiorbicular high angle scatter light transmission region along center line symmetrical setting
The semicircular low angle scattering light transmission region of side is arranged concentrically with semiorbicular high angle scatter light transmission region.
Further, photoelectronic detecting array part includes substrate and the low angle photodetector and the angle of elevation that are disposed on the substrate
Photodetector, angle of elevation photodetector are annular, and low angle photodetector is circle, and positioned at the angle of elevation photodetection of annular
Among device, low angle photodetector is corresponding with low angle scattering light transmission region, and angle of elevation photodetector and high angle scatter light are saturating
It is corresponding to penetrate region.
Further, the thickness of diaphragm is 0.15mm~0.25mm, and two low angles scatter the spacing between light transmission regions
Width is 0.85mm~0.94mm, a diameter of 4mm~5mm of low angle scattering light transmission region, high angle scatter light transmission region it is interior
Footpath is 5.5mm~6.5mm, and the outer diameter of high angle scatter light transmission region is 12.5mm~13.5mm.
Further, the scattering optical range that low angle photodetector can gather is respectively 1.1 °~5.9 °, angle of elevation photoelectricity
The scattering optical range that detector can gather is respectively 7 °~16 °.
It applies the technical scheme of the present invention, by setting flat-top optical element between laser and focus lamp, can will swash
The light that light device is sent is converted into the light that Energy distribution is more uniformly distributed, so that improving in light shared by high amplitude light
Proportion.And then the cell in blood is can guarantee in any position of sample flow, the light of laser assembly can be illuminated uniformly,
So as to improve to the resolution capability of haemocyte and cluster property.So, optics in blood cell analysis can also be reduced to fill
The requirement to fluid path is put, reduces complete machine cost.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
Below with reference to figure, the present invention is described in further detail.
Description of the drawings
The Figure of description for forming the part of the present invention is used for providing a further understanding of the present invention, and of the invention shows
Meaning property embodiment and its explanation do not constitute improper limitations of the present invention for explaining the present invention.In the accompanying drawings：
Fig. 1 shows the overall structure signal of the embodiment of the Optical devices according to the present invention for blood cell analysis
Figure；
Fig. 2 shows the internal structure schematic diagram of the laser assembly of the Optical devices of Fig. 1；
Fig. 3 shows the structure diagram of the diaphragm of the scattering light-receiving component of the Optical devices of Fig. 1；
Fig. 4 shows the structure diagram of the photoelectronic detecting array part of the scattering light-receiving component of the Optical devices of Fig. 1；
Fig. 5 shows the light spot shape that scattering light-receiving component of the prior art receives；
Fig. 6 shows the distribution of amplitudes figure for the light that scattering light-receiving component of the prior art receives；
Fig. 7 shows the light spot shape that the scattering light-receiving component of the present invention receives；
Fig. 8 shows the distribution of amplitudes figure for the light that the scattering light-receiving component of the present invention receives.
Wherein, above-mentioned attached drawing is marked including the following drawings：
10th, laser assembly；11st, laser；12nd, collimation lens；13rd, flat-top optical element；14th, focus lamp；20th, sample is whole
Flow component；30th, light-receiving component is scattered；31st, fixing bracket；32nd, diaphragm；321st, low angle scattering light transmission region；322nd, the angle of elevation
Scatter light transmission region；33rd, photoelectronic detecting array part；331st, low angle photodetector；332nd, angle of elevation photodetector；333rd, base
Plate.
Specific embodiment
It should be noted that in the case where there is no conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.The present invention will be described in detail below with reference to the accompanying drawings and in conjunction with the embodiments.
In order to which those skilled in the art is made to more fully understand the present invention program, below in conjunction in the embodiment of the present invention
The technical solution in the embodiment of the present invention is clearly and completely described in attached drawing, it is clear that described embodiment is only
The embodiment of a part of the invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill people
Member's all other embodiments obtained without making creative work should all belong to the model that the present invention protects
It encloses.
It should be noted that term " first " in description and claims of this specification and above-mentioned attached drawing, "
Two " etc. be the object for distinguishing similar, without being used to describe specific order or precedence.It should be appreciated that it so uses
Term can exchange in the appropriate case, so as to the embodiment of the present invention described herein.In addition, term " comprising " and " tool
Have " and their any deformation, it is intended that cover it is non-exclusive include, for example, containing series of steps or unit
Process, method, system, product or equipment are not necessarily limited to those steps or unit clearly listed, but may include without clear
It is listing to Chu or for the intrinsic other steps of these processes, method, product or equipment or unit.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singulative
It is also intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " bag
Include " when, indicate existing characteristics, step, operation, device, component and/or combination thereof.
Fig. 1 show the present invention the Optical devices for blood cell analysis embodiment, the Optical devices including according to
Laser assembly 10, sample rectifier stack 20 and the scattering light-receiving component 30 of secondary spread configuration.Laser assembly 10 is used to send out
Go out light irradiation sample rectifier stack 20, scattering light-receiving component 30 is for receiving from sample rectifier stack 20 transmitted through the light come
Line.As shown in Fig. 2, laser assembly 10 includes laser 11, flat-top optical element 13 and focus lamp 14, laser 11 is used to send
Light, focus lamp 14 are oppositely arranged with laser 11, and flat-top optical element 13 is arranged between laser 11 and focus lamp 14.It focuses on
For being focused to light, flat-top optical element 13 is uniformly distributed mirror 14 for the light that laser 11 is sent to be converted into energy
Light.
It applies the technical scheme of the present invention, it, can by setting flat-top optical element 13 between laser 11 and focus lamp 14
The light that Energy distribution is more uniformly distributed is converted into the light for sending laser 11, so that improving high amplitude light in light
Proportion shared by line.And then the cell in blood is can guarantee in any position of sample flow, the light of laser assembly 10 can
Uniform illumination, so as to improve to the resolution capability of haemocyte and cluster property.So, blood cell point can also be reduced
Requirement of the Optical devices to fluid path in analysis, reduces complete machine cost.
As shown in Fig. 2, as a preferred embodiment, laser assembly 10 further includes collimation lens 12, collimation is saturating
Mirror 12 is arranged between flat-top optical element 13 and laser 11, and collimation lens 12 is used to carry out the light that laser 11 is sent accurate
Directly.It is collimated by the light sent to laser 11, more the gathering of light can be allowed, avoid its diverging.Preferably, exist
In the technical solution of the present embodiment, collimation lens 12 is aspheric collimation lens.Optionally, the numerical aperture of collimation lens 12 is big
In or equal to 0.5.
As an alternative embodiment, the focal length of focus lamp 14 is 40mm~60mm.In the present embodiment, focus lamp
14 focal length is 50mm.
Using the technical solution of the present embodiment, the sample flow formed in sample rectifier stack 20 is about in 25um width, is adopted
With above-mentioned parameter, as shown in fig. 7, the flat of about 200um × 25um can be formed in the pattern detection district center of sample rectifier stack
Top light spot；As shown in figure 8, in the flat-top hot spot, high amplitude light institute accounting can reach the 20% of spot size.And existing
Have in technology, Gaussian spot as shown in Figure 5 can be obtained, in the Gaussian spot, high amplitude light institute accounting can only reach
To the 5% of spot size.Therefore, technical solution using the present invention, the illumination that laser light source can be more uniformly distributed, so as to improve
Resolution capability and cluster property to haemocyte.
As shown in Figure 1, in the technical solution of the present embodiment, scattering light-receiving component 30 includes fixing bracket 31, diaphragm
32 and photoelectronic detecting array part 33.Diaphragm 32 is mounted on fixing bracket 31, and adjoining with sample rectifier stack 20.Photoelectricity is visited
33 peace of array part is surveyed to turn in fixing bracket 31, and it is mutually separate compared with diaphragm 32 and sample rectifier stack 20.Diaphragm 32 is to sample
The light that rectifier stack 20 transmits is handled, and the light that photoelectronic detecting array part 33 then will transmit through diaphragm 32 is converted into telecommunications
Number.As shown in figure 3, as an alternative embodiment, along center line pair there are two being formed in the present embodiment, on diaphragm 32
Claiming the semicircular low angle scattering light transmission region 321 set and formation, there are two the semi-circulars along center line symmetrical setting
High angle scatter light transmission region 322.Semicircular low angle scattering light transmission region 321 and semi-ring positioned at center line the same side
The high angle scatter light transmission region 322 of shape is arranged concentrically.Direct laser can effectively be eliminated using the diaphragm 32 of the structure.
As shown in figure 4, photoelectronic detecting array part 33 includes substrate 333 and the low angle photodetection being arranged on substrate 333
Device 331 and angle of elevation photodetector 332.As a preferred embodiment, in the technical solution of the present embodiment, the angle of elevation
Photodetector 332 for annular, low angle photodetector 331 for circle, and positioned at annular angle of elevation photodetector 332 it
In, low angle photodetector 331 is corresponding with low angle scattering light transmission region 321, angle of elevation photodetector 332 and high angle scatter
Light transmission region 322 is corresponding.By the way that low angle photodetector 331 is provided in round, two and half can be corresponded better to
Circular low angle scattering light transmission region 321.By the way that angle of elevation photodetector 332 is arranged to annular, can preferably correspond to
In two semiorbicular high angle scatter light transmission regions 322.In the inventive solutions, by the way that photodetector is set
For the shape being more adapted with scattering light transmission region, more sufficient collection can be carried out to the scattering pipeline by diaphragm,
And then better blood cell differential.Optionally, substrate 333 is pcb board.
Optionally, the thickness of diaphragm 32 is 0.15mm~0.25mm, between two low angles are scattered between light transmission regions 321
It is 0.85mm~0.94mm away from width, a diameter of 4mm~5mm of low angle scattering light transmission region 321, high angle scatter light transmission region
322 internal diameter is 5.5mm~6.5mm, and the outer diameter of high angle scatter light transmission region 322 is 12.5mm~13.5mm.In this implementation
In the technical solution of example, the thickness of diaphragm 32 is 0.2mm, and the spacing width that two low angles are scattered between light transmission regions 321 is
0.89mm, a diameter of 4.76mm of low angle scattering light transmission region 321, the internal diameter of high angle scatter light transmission region 322 are
6.1mm, the outer diameter of high angle scatter light transmission region 322 is 13.2mm.
Optionally, the scattering optical range that low angle photodetector 331 can gather is respectively 1.1 °~5.9 °, angle of elevation photoelectricity
The scattering optical range that detector 332 can gather is respectively 7 °~16 °.
In scattering light-receiving components, traditional scattering light collection assembly has been divided into two independent parts, it is necessary to divide
It is other that diaphragm and photodetector are adjusted, and low angle and the angle of elevation are the subregion that has received of selectivity, poor signal quality,
Complicated, detection data are not comprehensive.It also scatters light and collects and light path is divided by two-way reception using Amici prism, cause light
System is huge, it is necessary to which the component adjusted is more, and the step very complicated in installation and adjustment manufactures cost and working service cost
It is high.And scattering light-receiving component 30 using the present invention, it need not set and extra receive eyeglass.It is small that collection can be maximized simultaneously
Angle and large-angle scattered light, it is no longer necessary to additional eyeglass reduces light loss, and light channel structure is simple, at low cost, easy to adjust,
Signal source is stablized, and accuracy is high.
It should be noted that the Optical devices for blood cell analysis of the present invention are particularly suitable for five classification haemocytes
Analysis.
Unless specifically stated otherwise, the component and positioned opposite, the digital table of step otherwise illustrated in these embodiments
It is not limited the scope of the invention up to formula and numerical value.Simultaneously, it should be appreciated that for ease of description, each portion shown in attached drawing
The size divided not is to be drawn according to actual proportionate relationship.For technology, side known to person of ordinary skill in the relevant
Method and equipment may be not discussed in detail, but in the appropriate case, technology, method and apparatus should be considered as authorizing specification
A part.It is shown here and discuss all examples in, any occurrence should be construed as merely it is illustrative rather than
As limitation.Therefore, the other examples of exemplary embodiment can have different values.It should be noted that：Similar label and word
Mother represents similar terms in following attached drawing, therefore, once it is defined in a certain Xiang Yi attached drawing, then in subsequent attached drawing
It need not be further discussed.
For ease of description, spatially relative term can be used herein, as " ... on ", " ... top ",
" ... upper surface ", " above " etc., for describing such as a device shown in the figure or feature and other devices or spy
The spatial relation of sign.It should be appreciated that spatially relative term is intended to comprising the orientation except device described in figure
Outside different azimuth in use or operation.For example, if the device in attached drawing is squeezed, it is described as " in other devices
It will be positioned as " under other devices or construction after the device of part or construction top " or " on other devices or construction "
Side " or " under other devices or construction ".Thus, exemplary term " ... top " can include " ... top " and
" in ... lower section " two kinds of orientation.The device can also other different modes positioning (being rotated by 90 ° or in other orientation), and
And respective explanations are made to the opposite description in space used herein above.
In the description of the present invention, it is to be understood that the noun of locality such as " forward and backward, upper and lower, left and right ", " laterally, vertical,
Vertically, orientation or position relationship indicated by level " and " top, bottom " etc. are normally based on orientation or position shown in the drawings and close
System is for only for ease of the description present invention and simplifies description, and in the case where not making explanation on the contrary, these nouns of locality do not indicate that
There must be specific orientation with the device or element for implying meaning or with specific azimuth configuration and operation, therefore cannot manage
It solves as limiting the scope of the invention；The noun of locality " inside and outside " refers to compared with inside and outside each component profile in itself.
The foregoing is only a preferred embodiment of the present invention, is not intended to limit the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
- A kind of 1. Optical devices for blood cell analysis, which is characterized in that the laser assembly including being arranged in order setting (10), sample rectifier stack (20) and scattering light-receiving component (30), the laser assembly (10) is for the irradiation that emits beam The sample rectifier stack (20), the scattering light-receiving component (30) transmit for receiving from the sample rectifier stack (20) The light to come over；The laser assembly (10) includes：Laser (11), for emitting beam；Focus lamp (14) is oppositely arranged with the laser (11), for being focused to light；Flat-top optical element (13) is arranged between the laser (11) and the focus lamp (14), the flat-top optical element (13) it is used to the light that the laser (11) is sent being converted into the equally distributed light of energy.
- 2. the Optical devices according to claim 1 for blood cell analysis, which is characterized in that the laser assembly (10) collimation lens (12) is further included, the collimation lens (12) is arranged on the flat-top optical element (13) and the laser (11) between, the light that the collimation lens (12) is used to send the laser (11) collimates.
- 3. the Optical devices according to claim 2 for blood cell analysis, which is characterized in that the collimation lens (12) it is aspheric collimation lens.
- 4. the Optical devices according to claim 3 for blood cell analysis, which is characterized in that the collimation lens (12) numerical aperture is greater than or equal to 0.5.
- 5. the Optical devices according to claim 1 for blood cell analysis, which is characterized in that the focus lamp (14) Focal length be 40mm~60mm.
- 6. the Optical devices according to claim 1 for blood cell analysis, which is characterized in that the scattering light-receiving Component (30) includes：Fixing bracket (31)；Diaphragm (32), on the fixing bracket (31), and it is adjoining with the sample rectifier stack (20)；Photoelectronic detecting array part (33), peace turn in the fixing bracket (31), and compared with the diaphragm (32) and the sample Rectifier stack (20) is mutually separate.
- 7. the Optical devices according to claim 6 for blood cell analysis, which is characterized in that on the diaphragm (32) Along the semicircular low angle scattering light transmission region (321) of center line symmetrical setting and formation, there are two edges there are two being formed The semiorbicular high angle scatter light transmission region (322) of the center line symmetrical setting, half positioned at described center line the same side The circular low angle scattering light transmission region (321) with the semiorbicular high angle scatter light transmission region (322) is concentric sets It puts.
- 8. the Optical devices according to claim 7 for blood cell analysis, which is characterized in that the photodetection battle array Row part (33) includes substrate (333) and the low angle photodetector (331) and the angle of elevation photoelectricity that are arranged on the substrate (333) Detector (332), the angle of elevation photodetector (332) are annular, and the low angle photodetector (331) is circular, and position Among the angle of elevation photodetector (332) of the annular, the low angle photodetector (331) and the low angle scattering light are saturating It is corresponding to penetrate region (321), the angle of elevation photodetector (332) is corresponding with the high angle scatter light transmission region (322).
- 9. the Optical devices according to claim 7 for blood cell analysis, which is characterized in that the diaphragm (32) Thickness is 0.15mm~0.25mm, the spacing width between two low angles scattering light transmission regions (321) for 0.85mm~ 0.94mm, a diameter of 4mm~5mm of the low angle scattering light transmission region (321), the high angle scatter light transmission region (322) internal diameter is 5.5mm~6.5mm, and the outer diameter of the high angle scatter light transmission region (322) is 12.5mm~13.5mm.
- 10. the Optical devices according to claim 8 for blood cell analysis, which is characterized in that the low angle photoelectricity The scattering optical range that detector (331) can gather is respectively 1.1 °~5.9 °, and the angle of elevation photodetector (332) can adopt The scattering optical range of collection is respectively 7 °~16 °.
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| CN201711087648.7A CN108120666A (en) | 2017-11-07 | 2017-11-07 | For the Optical devices of blood cell analysis |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711087648.7A CN108120666A (en) | 2017-11-07 | 2017-11-07 | For the Optical devices of blood cell analysis |
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| CN201711087648.7A Pending CN108120666A (en) | 2017-11-07 | 2017-11-07 | For the Optical devices of blood cell analysis |
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| CN113267840A (en) * | 2021-05-08 | 2021-08-17 | 中国工程物理研究院激光聚变研究中心 | Sawtooth diaphragm, application thereof and debugging method of sawtooth diaphragm to light path |
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Application publication date: 20180605 |