CN108078805A - Preserving moisture and protecting skin water and preparation method thereof - Google Patents
Preserving moisture and protecting skin water and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of preserving moisture and protecting skin water, contain dextran and sweet tea polyphenols.Inventor is creative to carry out compounding by dextran and precious jade mountain sweet tea polyphenols and is used to prepare preserving moisture and protecting skin water, and the preserving moisture and protecting skin water of research and development has the characteristics that moistening effect is good, safe, comfortable, non-stimulated.Experiments have shown that products obtained therefrom can be effectively improved pachylosis chronic eczema, the defects of existing moisturizing aqueous systems are unstable, and irritation is strong, and water humidity is inadequate is overcome, higher degree meets consumer demand.
Description
Technical field
The invention belongs to Moisturizer technical fields more particularly to a kind of preserving moisture and protecting skin water and preparation method thereof.
Background technology
Cosmetics are both an industry and a kind of culture, it is multi-disciplinary crystallization.With fine chemistry industry, biological section
It learns, the rapid development of material science, deep and human genome project startup of the cell science in dermatology, cosmetics
The trend of industry will increasingly towards more science, natural, safely effectively direction is developed.Due to the continuous hair of science and technology
Exhibition, it has been found that artificial synthesized chemicals is easier than extracting natural product, manufactures and use is also more convenient, therefore Synthetic artifact
It is increasing, very big impetus has been risen to the development of cosmetics.However, not only consume largely can not be again for synthesis of chemicals
The raw or very slow resource of regenerative process, and its a large amount of discarded object are brought to nature, it is original including nature
Without discarded object, caused environmental pollution attracted people's attention.With the development of society, people are to healthy living side
The interest of formula is increasingly enhanced, and natural product has been struck fire into the people's hearts.Consumer is for keeping the emerging of body ＆ mind health aspect
Interest is increasing, this directly results in increasing substantially for natural personal care market, fast so as to the tendency of " going back to nature "
Speed involves entire cosmetic industry.
Natural moisture preserving system in human skin is mainly made of water, lipid, natural moisturizing factor.In cuticula, energy
Enough make that water is attached to intracellular hygroscopicity and water-soluble substances is referred to as natural moisturizing factor.Positioned at the outermost cutin of skin
Layer plays an important role in terms of human body resists extraneous invasion and attack.Water content of stratum corneum skin condition at 10%~20% is preferable,
If water content is less than 10%, the easily dry even furfur of skin.So-called moisturizing refers to by preventing the loss of moisture and suction in skin
The moisture of external environment is received to achieve the purpose that in skin containing moisture.Cosmetics with moisture-keeping efficacy are with balance skin water
On the one hand the effect divided supplements the oil components of skin and hydrophilic moisturizing ingredient, on the other hand as active ingredient quilt
Skin absorbs, and achievees the purpose that skin water and oil balance.Existing moisturizer is not strong there is moisture-retaining capacity in the market, irritation
By force, the problems such as easy allergy is red and swollen, therefore, the safe natural activity factor show the powerful market vitality, become cosmetics
The direction of field future development.
Dextran scientific name glucan is a kind of homotype exocellular polysaccharide being made of completely α-D- glucopyra sugar monomers,
It is mainly fermented and generated through Leuconostoc mesenteroides by sucrose, because it has very strong positive optical, so being referred to as " dextran ".
Dextran is white or off-white color amorphous powder, and odorless, tasteless is soluble easily in water, and insoluble in ethyl alcohol, aqueous solution is colourless
Or the clear liquid of micro-strip opalescence.Dextran is one of blood substitutes optimal at present, can prevent red blood cell and blood platelet
Aggregation reduces viscosity of blood, so as to play the role of improving microcirculation, is also used for eyedrops field.The study found that dextrose
Hydroxyl, carboxyl and other polar groups in acid anhydride molecule can form hydrogen bond with hydrone and combine large quantity of moisture, between strand
It is interweaved and reticulates, complement each other with hydrogen bond and play good water retention.In addition, in extracellular matrix, dextran with
Other polysaccharide components and fibrous proteins in skin collectively constitute the extracellular gelatinised matrix containing large quantity of moisture, are provided for skin
Moisture.Further, since dextran belongs to polysaccharide, there is good film forming, one layer can be formed uniformly in skin surface
Film to reduce skin surface moisture evaporation, activates body cell, prevents pachylosis and aging.
Sweet tea polyphenols play an important role of skin-active, radical scavenging activity by being extracted in the Sweet tea of Guangxi characteristic resources precious jade mountain
Power is good, and almost inhibits precipitation of the leukotrienes from stomach wall cell, alleviates histamine release, has excellent anti-oxidant
And anti-allergic effects.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of moistening effect is good, safety and comfort and non-stimulated preserving moisture and protecting skin
Water and preparation method thereof.
In order to solve the above technical problems, the present invention uses following technical scheme：
Preserving moisture and protecting skin water, contains dextran and sweet tea polyphenols.
The weight ratio of dextran and sweet tea polyphenols is (1~6): (1~3).
The weight ratio of dextran and sweet tea polyphenols is 3: 2.
Count by weight percentage, the content of dextran is 1%~5%.
The molecular weight ranges of dextran are 105~106Da。
The molecular weight of dextran is 100kDa.
Above-mentioned preserving moisture and protecting skin water, is made of following raw materials by weight：Dextran 1 %~5%, sweet tea tannins
0.5%~3%, sealer 0.1%~0.5%, water absorbing agent 0.4%~8%, ethyl alcohol 1%, preservative 0.2%, surplus for go from
Sub- water.
Above-mentioned preserving moisture and protecting skin water, is made of following raw materials by weight：Dextran 3%, sweet tea tannins 2%, envelope
Agent 0.2%, water absorbing agent 6% are closed, ethyl alcohol 1%, preservative 0.2%, surplus is deionized water.
Sealer is water-soluble jojoba oil；Water absorbing agent includes glycerine and butanediol, and the weight ratio of glycerine and butanediol is 2
∶1；Preservative is Phenoxyethanol.
The preparation method of above-mentioned preserving moisture and protecting skin water, comprises the following steps：：
(1) dextran, sweet tea polyphenols, water-soluble jojoba oil are dissolved in suitable quantity of water successively in proportion；
(2) glycerine, butanediol, ethyl alcohol, Phenoxyethanol are sequentially added into mixing in suitable quantity of water in proportion；
(3) two parts aqueous solution of step (1) and (2) is mixed and shaken up to get preserving moisture and protecting skin water.
At present, application of the dextran in cosmetics is still blank, and dextran has good moisture absorbability and moisture retentivity
And a kind of good film forming and be safe and non-stimulating polysaccharide；Precious jade mountain sweet tea polyphenols are the institutes from the Sweet tea of Guangxi specialty precious jade mountain
It takes, there are good inoxidizability and anti-aging effects, and with certain biocidal property.Inventor it is creative by dextran
Compounding is carried out with precious jade mountain sweet tea polyphenols and is used to prepare preserving moisture and protecting skin water, and the preserving moisture and protecting skin water of research and development has that moistening effect is good, peace
Entirely, the characteristics of comfortable, non-stimulated.Experiments have shown that products obtained therefrom can be effectively improved pachylosis chronic eczema, existing moisturizing is overcome
The defects of aqueous systems are unstable, and irritation is strong, and water humidity is inadequate, higher degree meet consumer demand.
Description of the drawings
Fig. 1 is the measure flow chart of dextransucrase enzyme activity.
Fig. 2 is the measure flow chart of dextran enzyme activity.
Fig. 3 is the hygroscopicity comparison diagram of different molecular weight dextran and control group, in figure：a：RH is 43%；b：RH is
81%.
Fig. 4 is the moisture retention comparison diagram of different molecular weight dextran and control group, in figure：a：RH is 43%；b：RH is
81%.
Fig. 5 is dextran to DPPH elimination effect figures.
Fig. 6 is dextran to Hydroxyl radical-scavenging design sketch.
Fig. 7 is elimination effect figure of the dextran to superoxide ion.
Fig. 8 is chelate effect figure of the dextran to iron ion.
Fig. 9 is inhibiting rate result figure of the dextran to hyaluronidase.
Figure 10 is dextran autostimulation result figure.
Figure 11 is elimination effect comparison diagram of the combination solution with sweet tea tannins to DPPH.
Figure 12 is combination solution and sweet tea tannins to OH-Elimination effect comparison diagram.
Figure 13 is combination solution and sweet tea tannins to O2Elimination effect comparison diagram.
Figure 14 is chelant ability comparison diagram of the combination solution with sweet tea tannins to iron ion.
Figure 15 is inhibition comparison diagram of the combination solution with sweet tea tannins to hyaluronidase.
Figure 16 is combination solution and sweet tea tannins anti-irritant effect comparison diagram.
Specific embodiment
The ingredient explanation of preserving moisture and protecting skin water of the present invention
Glycerine and butanediol are combined as water absorbing agent, and glycerine has water sorption, can be used to adsorb in Moisturizer
Hydrone in air makes it be covered in keratoderma and keeps moistening, and butanediol is the polyalcohols being gentle, in skin care
Small molecule moisturizing ingredient is commonly used in product, moisture cuticula can be stayed in, there is good hygroscopicity, it is absorbable to be equivalent to matter itself
The moisture of 12.5% (relative humidity 50%) and 38.5% (relative humidity 80%) is measured, while there is bacteriostasis.
Dextran is as moisturizer.Hydroxyl, carboxyl isopolarity group can form hydrogen with hydrone in dextran molecule
Key and with reference to large quantity of moisture, while intermolecular intertexture reticulates, and can firmly lock moisture, play very strong moisture-keeping function.In addition,
In extracellular matrix, dextran collectively constitutes the born of the same parents containing large quantity of moisture with other polysaccharide components in skin and celloglobulin
Outer gelatinised matrix avoids viscous sense and greasy feeling that sun-screening agent is brought.Dextran belongs to polysaccharide, has good film forming,
Smooth and uniform film can be formed in skin surface so that moisture permeates into cuticula from substrate microstructure, induces cuticula into one
Aquation is walked, prevents moisture of skin from evaporating.
Sweet tea polyphenols, by being extracted in the Sweet tea of Guangxi characteristic resources precious jade mountain, have and live as bacteriostatic agent and antioxidant
The effect of skin, radical scavenging activity is good, and almost inhibits precipitation of the leukotrienes from stomach wall cell, alleviates histamine
Release has excellent anti-oxidant and anti-allergic effects.
Water-soluble jojoba oil is very similar to our skins point as sealer, the structure and chemical property of jojoba oil
The sebum secreted, applied to skin, can stablize sebum layer, and unimpeded pore adjusts the fat secretion of oiliness or Combination skin, changes
Kind skin inflammation, sensitivity, eczema, chronic eczema etc..It easily absorbs after smearing, bellding light and is not formed on dry sensitive skin strong
The soft protective film of health silk.
First, the screening of dextran molecule amount
1st, dextran molecule amount and moisturizing, the dose-effect relationship of hygroscopic effect
As shown in figure 3, under the environment dried in relative humidity 43%, partially, the wettability power of dextran and control group：It is sweet
Oily ＞ Dex-2000 ＞ Dex-1000 ＞ Dex-500 ＞ Dex-250, Dex-100, trehalose, hyaluronic acid.In relative humidity
81%th, partially in the environment of moistening, the wettability power of dextran and control group：Dex-2000 ＞ glycerine ＞ Dex-1000 ＞ Dex-
500 ＞ trehalose ＞ Dex-100, Dex-250 ＞ hyaluronic acids.It can be seen that in the environment of opposite moistening, macromolecule is right
The moisture sorption effect of the sugared acid anhydride (Dex-2000 and Dex-1000) of rotation is more preferable and suitable with moisture absorption effect of traditional hygroscopic agent glycerine.In
Small-molecular-weight dextran (Dex-500, Dex-250, Dex-100) although wettability power and persistence not as good as Dex-2000 and
Dex-1000, but still show potent wettability power.From data analysis, small molecular amount dextran (Dex-500, Dex-
250th, Dex-100) moisture absorption effect and trehalose it is close, in relative humidity 81%, moisture absorption effect be better than hyaluronic acid.
As shown in figure 4, under the environment dried in relative humidity 43%, partially, the moisture-retaining capacity of dextran and control group：Thoroughly
Bright matter acid ＞ Dex-2000 ＞ Dex-1000 ＞ Dex-500, Dex-250, Dex-100, trehalose ＞ glycerine.In relative humidity
81%th, partially in the environment of moistening, the moisture-retaining capacity of dextran and control group：Hyaluronic acid Dex-2000 ＞ Dex-1000 ＞
Dex-500, Dex-250, Dex-100, trehalose ＞ glycerine.The moisturizing rate of the dextran of different molecular weight is above trehalose
And glycerine, it is not much different with the moisture-keeping efficacy of hyaluronic acid, is either all shown in the environment of dry or moistening excellent
Good moisture retention.
It can be seen that dextran has effects that excellent moisture-absorbing moisture-keeping, reason is in the hydrophilic radical in its structure
Quantity and its hydrophilic power, such as traditional water-retaining agent glycerine, regard base material of cosmetics due to it has very strong moisture sorption effect
Be widely used in all types of products, containing only substantial amounts of hydroxyl in its structure, although hydroxyl with very strong water absorbing capacity, but with
The Hydrogen bonding forces that hydrone is formed are but very weak, therefore its moistening effect is far from, dextran and hyaluronic acid are good.And dextrose
There are a large amount of aldehyde radicals and carboxyl isopolarity group in the molecular structure of acid anhydride, can and water molecules, and form the combination power of hydrogen bond
Relatively strong not easy to break, crosslinking is in net structure mutually between hydrogen bond, and therefore, the dextran of different molecular weight shows good
Moisture absorption and performance of keeping humidity, moisture-absorbing moisture-keeping is delay skin aging, the essential condition for ensureing skin health, therefore dextran possesses
As the potentiality of cosmetics moisturizing base-material, cosmetic industry can be applied to.
2nd, the dose-effect relationship of dextran molecule amount and oxidation
As shown in Fig. 5 to Fig. 8, analyzed in terms of dextran molecule amount, the antioxygen of small-molecular-weight dextran Dex-100
Change ability is outstanding in all molecular weight dextran samples, exemplified by removing DPPH free radicals, when Dex-100's
When concentration is 30mg/mL, reach 59.36% to the clearance rate of DPPH, and the oxidation resistance of macromolecule dextran then phase
To poor, such as Dex-2000 and Dex-1000, when concentration is 30mg/mL, the clearance rate of DPPH is only 38.32% He
39.65%.
It analyzes, just has when the concentration of dextran is more than 10mg/mL to free radical relatively apparent in terms of solution concentration
Scavenging activity；When concentration is in the range of 10mg/mL~20mg/mL, the dextran of each molecular weight fragment is to free radical
Scavenging activity is weaker, to the clearance rate of free radical all in 40% or so, when concentration is more than 20mg/mL, small molecular amount dextrorotation
Sugared acid anhydride such as Dex-100, Dex-250 etc. just have stronger Scavenging activity to free radical.
From the structural analysis of dextran, active hydrogen molecule is mainly derived from hemiacetal carbon atom and work in primary structure
Bold and vigorous hydroxyl.When molecular weight is smaller, polysaccharide is more soluble in water, and the space structure of molecule can be unfolded, and active group is easy
It is combined in free radical, the corresponding enhancing of activity of polysaccharide molecule, so as to enhance its oxidation resistance.
3rd, dextran molecule amount and antiallergy, the dose-effect relationship of anti-irritant effect
As shown in Fig. 9 to Figure 10, with the increase of dextran solution concentration, hyaluronic acid enzyme inhibition rate is constantly rising
It is high.But when concentration reaches 40mg/mL, hyaluronic acid enzyme inhibition rate just higher than standard 30% is effectively inhibited, works as solution concentration
When increasing to 50mg/mL, hyaluronic acid enzyme inhibition rate is only 40% or so.It can be seen that the dextran of different molecular weight is simultaneously
There is no apparent antiallergy ability.The dextran of the different molecular weight irritation of itself is not with concentration in reaction system
Increase and raise.
The dextran of five kinds of molecular weight is ranked up according to the height of hemolysis rate, is followed successively by：Dex-2000 ＞ Dex-
1000 ＞ Dex-500 ＞ Dex-25 ＞ Dex-100.When concentration reaches 50mg/mL, the dextran of different molecular weight is not sent out
Raw apparent erythrocyte haemolysis, hemolysis rate are no more than 10%.It is indicated above that the irritation of dextran and its molecule
Amount acts on skin without obvious stimulation, securely and reliably, base material of cosmetics can be used as to be applied in cosmetics without obvious relation.
2nd, the effect of dextran and sweet tea polyphenols compound
Sweet tea extract of the sweet tea polyphenols used in the present invention from Guangxi specialty precious jade mountain Sweet tea, wherein, sweet tea tannins >=
40%, Guilin collection fine jade strength natural goods Science and Technology Ltd. provides.
1st, the anti-oxidation efficacy of dextran and sweet tea polyphenols compositional liquor
As shown in Figure 11 to 14, the combination solution of sweet tea polyphenols and dextran has the energy for relatively removing free radical by force
Power, i.e. antioxidation activity are high.By with sweet tea polyphenols Oxidation Resistance Test data comparison, find the dextran of different molecular weight
The bioactivity unrestraint of sweet tea polyphenols is acted on, the free radical scavenging activity of combination solution is compared with sweet tea polyphenols solution mean height
5%~10%, when the concentration of sweet tea polyphenols in combination solution reaches 30mg/mL, free radical is clear in three kinds of test methods
Except rate is up to 95%.It is worth noting that, after the dextran of different molecular weight is added in, during Oxidation Resistance Test,
The difference of three groups of parallel test results becomes smaller, more stable compared with the parallel test result of sweet tea polyphenols.
2nd, antiallergy effect of dextran and sweet tea polyphenols compositional liquor
As shown in figure 15, it is seen that combination solution has stronger inhibitory action to hyaluronidase, shows that combination solution has
There is stronger antiallergy ability, and raised with concentration, antiallergy ability enhancing, when the concentration of sweet tea polyphenols in combination solution is
During 35mg/mL, the inhibiting rate of combination solution reaches more than 75%.Parallel test statistics indicate that, add in dextran can carry
The stability of high combination solution, the difference of parallel test reduce.
3rd, anti-irritant effect of dextran and sweet tea polyphenols compositional liquor
The SDS (neopelex) that experiment is chosen is surfactant, can stimulate erythrocyte, cause haemolysis
Rate increases.If the combination solution added in can reduce stimulations of the SDS to erythrocyte, reduce hemolysis rate and show that combination solution has
There is anti-irritant ability.As shown in figure 16, combination solution has effects that inhibit RBC hemolysis rates, and when concentration is 20mg/mL,
Inhibiting rate has reached more than 60%, and it is apparent to inhibit hemolyzing effect.In addition, the dextran of different molecular weight is pressing down compound system
Unrestraint acts on during SDS processed causes RBC hemolytic tests, and the inhibiting rate of combination solution and the inhibiting rate of sweet tea polyphenols are held substantially
It is flat.
How to implement for the present invention is furture elucidated, instance interpretation explanation exemplified below.The percentage arrived involved in embodiment
Number " % " if not specified, refers to mass percent, and the percentage of solution refers to the grams containing solute in 100ml, between liquid
Percentage, refer to the volume ratio of the solution at 25 DEG C.
In addition, dextran used is prepared via a method which to obtain：
One) preparation of culture medium and solvent
Solid medium：Sucrose 13g, KH2PO40.03g, Na2HPO40.14g, agar 2g, peptone 0.2g, distilled water
100mL, pH7.0~7.2,121 DEG C of high pressure steam sterilization 20min.
Seed culture medium：Sucrose 5g, KH2PO40.03g, Na2HPO40.14g, peptone 0.2g, yeast extract 1g, distilled water
100mL, pH6.8,121 DEG C of high pressure steam sterilization 20min.
Culture medium：Sucrose 5g, KH2PO40.1g, Na2HPO40.36g, yeast extract 1.25g, beef extract 2g, distilled water
100mL, pH7.0~7.2,121 DEG C of high pressure steam sterilization 20min.
DNS reagents：Solution A：3,5- dinitrosalicylics acid solution, the 150mL of 127.5g sodium potassium tartrate tetrahydrates, 440mL 1%
10%NaOH；Solution B：3.45g phenol is dissolved in 7.6mL 10%NaOH, adds 27mL distilled water, 3.45g sodium hydrogensulfites.It is molten
Up to DNS after liquid A, B mixing, store in brown bottle, room temperature preservation uses after 7 days.
Reagent A：8.25g K3Fe(CN)6And 10.60gNa2CO3With distillation water dissolution and 1L is settled to, dark place stands 2~3
It is used after it.
Reagent B：Take KI 25g, ZnSO4·7H2O 50g and NaCl 250g in beaker, with distillation water dissolution after be settled to
1L。
Reagent C：The dilution of glacial acetic acid 5mL distilled water is taken to be settled to 100mL.
Dextransucrase substrate specificity：20mM sodium acetates, 5% sucrose, glacial acetic acid adjust pH to 5.4.
Dextran zymolyte Dextran-70 solution：0.5g is taken to be dissolved in the Dex-70 of 105 DEG C of dry 4h super
In pure water and it is settled to 50mL.
Starch indicator：1g soluble starches are fully dissolved with 200mL boiling water, are cooled to room temperature.
Ultra-pure water：Resistivity reaches 18.2M Ω*Cm, almost by gas, organic matter, thalline and the colloidal substance not dissociated
It removes to the water of very low degree.
Two) preparation and purifying of dextransucrase
The Leuconostoc mesenteroides being stored in ultra low temperature freezer is inoculated in solid slope culture medium, is trained in 25 DEG C of constant temperature
It supports and is activated for 24 hours in case, be inoculated on surface culture medium and cultivate under the same conditions for 24 hours, with oese picking single bacterium colony
It is inoculated on seed culture medium, culture is to logarithmic phase (about 20h) under 25 DEG C, 150r/min shaking tables, with liquid-transfering gun by 2% (v/
V) inoculum concentration is inoculated on culture medium, after cultivating 20h under the same conditions, in 4 DEG C, 12000r/min freeze at a high speed from
Heart 20min, obtains crude enzyme liquid.20mL dextransucrase crude enzyme liquids is taken to be placed in 50mL centrifuge tubes, add in the poly- of 50% (w/w)
Ethylene glycol 6000 (PEG) solution 16mL, then it is 40g to complement to quality with sterile water.Abundant mixing, which is placed in 4 DEG C of refrigerators, to be stood
After 12h, in 4 DEG C, 20min is centrifuged under the conditions of 12000r/min, removes dextransucrase of the supernatant to get purifying.
Three) enzyme activity determination
The measure of dextransucrase enzyme activity：The dextransucrase unit interval is measured using DNS reducing sugar methods
Interior catalysing sucrose decomposes the amount of generation fructose, and is defined under the conditions of 25 DEG C, and catalysis substrate sucrose generates 1 μm of ol fructose in 1min
Required enzyme amount is an enzyme-activity unit (U).
In formula：
M---- fructose quality (mg)；M---- fructose molal weight 180.16 (g/mol)；The t---- reaction time (min).
Determination step such as Fig. 1.
The measure of dextran enzyme activity：It is defined as under the conditions of 25 DEG C, catalysis substrate Dextran-70 per minute is generated
With the enzyme amount of the required dextranase of the comparable reduced sugar of reducing power of 1 μm of ol sodium thiosulfate.
In formula：
Sodium thiosulfate amount needed for B---- titration blank controls, mL；
Sodium thiosulfate amount needed for T---- titration institute sample, mL；
The correction factor of F----0.01mol/L hypo solutions (in this experiment, F=1.18)
T---- reaction time, min.
Determination step such as Fig. 2.
Four) sucrose that concentration is 0.4mol/L is added to double enzyme enzyme activities than DN: DS (dextranase: dextran
Invertase) it is 1: 50, dextransucrase addition is in the 50mL reaction systems of 0.2U/mL, which is placed in
It is reacted in constant-temperature table, reaction condition temperature is 25 DEG C, and rotating speed 110r/min, time 50h is to get required zymotic fluid.
Five) by zymotic fluid through ultrafiltration membrance filter, then through vacuum freeze drying acquisition dry powder-shaped dextran.
After measured, dextran target molecular weight segment is 105~106Da, accounting 73.24%.
Embodiment 1
The formula (weight percent) of preserving moisture and protecting skin water：Dextran 1 %, sweet tea tannins 2%, water-soluble jojoba oil
0.2%th, glycerine 4%, butanediol 2%, Phenoxyethanol 0.2%, ethyl alcohol 1%, surplus are deionized water.
The preparation method of preserving moisture and protecting skin water：Dextran, sweet tea polyphenols, water-soluble jojoba oil are dissolved in successively in proportion suitable
It measures in water；Glycerine, butanediol, ethyl alcohol, Phenoxyethanol are sequentially added into mixing in suitable quantity of water in proportion；Two parts aqueous solution is mixed
Conjunction is shaken up to get preserving moisture and protecting skin water.
Embodiment 2
The formula (weight percent) of preserving moisture and protecting skin water：Dextran 3%, sweet tea tannins 0.5%, water-soluble jojoba oil
0.2%th, glycerine 4%, butanediol 2%, Phenoxyethanol 0.2%, ethyl alcohol 1%, surplus are deionized water.
Preparation method is the same as embodiment 1.
Embodiment 3
The formula (weight percent) of preserving moisture and protecting skin water：Dextran 3%, sweet tea tannins 2%, water-soluble jojoba oil
0.2%th, glycerine 4%, butanediol 2%, Phenoxyethanol 0.2%, ethyl alcohol 1%, surplus are deionized water.
Preparation method is the same as embodiment 1.
Embodiment 4
The formula (weight percent) of preserving moisture and protecting skin water：Dextran 3%, sweet tea tannins 3%, water-soluble jojoba oil
0.2%th, glycerine 4%, butanediol 2%, Phenoxyethanol 0.2%, ethyl alcohol 1%, surplus are deionized water.
Preparation method is the same as embodiment 1.
Embodiment 5
The formula (weight percent) of preserving moisture and protecting skin water：Dextran 5 %, sweet tea tannins 2%, water-soluble jojoba oil
0.2%th, glycerine 4%, butanediol 2%, Phenoxyethanol 0.2%, ethyl alcohol 1%, surplus are deionized water.
Preparation method is the same as embodiment 1.
Quality testing is evaluated
First, liquid subjective appreciation
1 to 5 sample of case study on implementation is taken, ambient sunlight direct projection and non-direct sunlight two states is respectively placed in, examines
The outer table status of sample.
2nd, resist cold, heat resistance assessment
It is tested with reference to the assay method in QB/T 1857-2004.
Cold resistance detects：Refrigerator is adjusted to -5 DEG C~-10 DEG C in advance, 1 to 5 sample of case study on implementation of Packing Intact is taken to put
It in refrigerator, takes out, is then complied with standard without apparent Traits change before visual observations, with experiment after recovering to room temperature afterwards for 24 hours.
Heat resistance detects：Constant incubator is adjusted to (40 ± 1) DEG C in advance, is weighed respectively in the culture dish weighed
1 to 5 sample of case study on implementation, takes out afterwards for 24 hours, then meets without apparent Traits change before visual observations, with experiment after recovering to room temperature
Standard.
3rd, skin sense is assessed
Select the age in the female volunteers 60 of 20-35 Sui or so as study subject, subject first pacifies in the room
Wait quietly treating 20-30min, so that skin reaches equilibrium state, then smear surfactant in subject's forearm respectively, application area is
4cm × 4cm, smearing quality are 0.5g.Score, be averaged according to every standards of grading in following table, by fraction from height to
It is low to be ranked up.
1 skin sense evaluation item of table and standard
4th, moisture absorbability and moisture retentivity is evaluated
Hygroscopic measure：In 25 DEG C of constant temperature, constant humidity is respectively to carry out under conditions of 43%, 81%.First by number
A specification is that the measuring cup of 60mm × 30mm is placed in 105 DEG C of baking ovens, fully dry to constant mass.Take 1 to 5 sample of case study on implementation
Product are respectively placed in and have dried into the measuring cup of constant mass, and measuring cup opening is placed in constant temperature and humidity incubator, are putting
It puts in 1~12h, claims quality respectively per 2h, quality (being accurate to 0.0001g) is claimed when placing for 24 hours, perseverance is put back at once after the completion of weighing
In constant temperature and humidity incubator.
Calculate hydroscopicity：
In formula：m0Quality/g of sample before-experiment；
mt- experiment carries out quality/g of sample after different time
The measure of moisture retention：It is selected in 25 DEG C of constant temperature, constant humidity is respectively to carry out under conditions of 43%, 81%.First
The glass plate that several specifications are 750mm × 750mm is placed in 105 DEG C of baking ovens, it is fully dry to constant, first weigh several post
The quality of the glass plate of 3cm adhesive plasters, records respectively；1 to 5 sample of case study on implementation of 200 μ L, sample solution drop are drawn with liquid-transfering gun
It is added on the glass plate for posting 3cm adhesive plasters, weighs quality (being accurate to 0.0001g) with assay balance, sample is smoothened, by glass
Plate be put into the constant humidity of requirement, temperature incubator in, it is parallel to do three groups；It is placing in 1~12h, is claiming quality respectively per 2h,
Claim quality (being accurate to 0.0001g) when placing for 24 hours, put back at once in constant temperature and humidity incubator after the completion of weighing.
Calculate moisturizing rate：
In formula：M0- hollow plate quality/g；
M1Glass sheet quality/g after-sample-adding；
M2-- quality/g after several hours is placed in constant temperature and humidity incubator.
5th, comprehensive grading standard
It is given a mark according to four above indexs, appearance character accounts for 15 points；The sample heat resistance that resists cold accounts for 25 points；Sample skin
Sense evaluation accounts for 40 points；Moisturizing, hygroscopicity evaluation respectively account for 10 points.Sample and formula are analyzed by calculating total score, specifically commented
Point detailed rules and regulations see the table below：
2 comprehensive grading table of table (requires inconsistent) with foregoing scoring
3 Product Quality Evaluation result of table (requires inconsistent) with foregoing scoring
The result shows that：The preserving moisture and protecting skin water performance of keeping humidity of the present invention is good.
Claims (10)
1. a kind of preserving moisture and protecting skin water, it is characterised in that contain dextran and sweet tea polyphenols.
2. preserving moisture and protecting skin water according to claim 1, it is characterised in that：The weight of the dextran and sweet tea polyphenols
Than for (1~6): (1~3).
3. preserving moisture and protecting skin water according to claim 2, it is characterised in that：The weight of the dextran and sweet tea polyphenols
Than for 3: 2.
4. preserving moisture and protecting skin water according to claim 1, it is characterised in that count by weight percentage, the content of dextran
For 1%~5%.
5. preserving moisture and protecting skin water according to claim 4, it is characterised in that：The molecular weight ranges of the dextran are 105~
106Da。
6. preserving moisture and protecting skin water according to claim 5, it is characterised in that：The molecular weight of the dextran is 100kDa.
7. preserving moisture and protecting skin water according to claim 6, it is characterised in that be made of following raw materials by weight：Dextrorotation
Sugared acid anhydride 1%~5%, sweet tea tannins 0.5%~3%, sealer 0.1%~0.5%, water absorbing agent 0.4%~8%, ethyl alcohol 1%,
Preservative 0.2%, surplus are deionized water.
8. preserving moisture and protecting skin water according to claim 7, it is characterised in that be made of following raw materials by weight：Dextrorotation
Sugared acid anhydride 3%, sweet tea tannins 2%, sealer 0.2%, water absorbing agent 6%, ethyl alcohol 1%, preservative 0.2%, surplus are deionized water.
9. preserving moisture and protecting skin water according to claim 8, it is characterised in that：The sealer is water-soluble jojoba oil；Institute
Stating water absorbing agent includes glycerine and butanediol, and the weight ratio of glycerine and butanediol is 2: 1；The preservative is Phenoxyethanol.
10. the preparation method of preserving moisture and protecting skin water according to claim 1, it is characterised in that comprise the following steps：：
(1) dextran, sweet tea polyphenols, water-soluble jojoba oil are dissolved in suitable quantity of water successively in proportion；
(2) glycerine, butanediol, ethyl alcohol, Phenoxyethanol are sequentially added into mixing in suitable quantity of water in proportion；
(3) two parts aqueous solution of step (1) and (2) is mixed and shaken up to get preserving moisture and protecting skin water.
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