CN108034609A - One plant of series bacillus bacterial strain T1-1 with phosphate solubilization and its application - Google Patents
One plant of series bacillus bacterial strain T1-1 with phosphate solubilization and its application Download PDFInfo
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- CN108034609A CN108034609A CN201711485168.6A CN201711485168A CN108034609A CN 108034609 A CN108034609 A CN 108034609A CN 201711485168 A CN201711485168 A CN 201711485168A CN 108034609 A CN108034609 A CN 108034609A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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Abstract
The invention belongs to microbial technology field, specifically discloses one plant of series bacillus bacterial strain T1 1 with phosphate solubilization and its application.The deposit number of the series bacillus bacterial strain T1 1 is CGMCC No.14927.The microbial bacterial agent or microbial manure prepared by such Bacillus strain, can have significant facilitation to the growth of vegetables, can effectively reduce the usage amount of chemical phosphatic ferfilizer, can be promoted the use of in agricultural production.
Description
Technical field
The invention belongs to microbial technology field, specifically, is related to one plant of series bacillus bacterium with phosphate solubilization
Strain and its application.
Background technology
Phosphorus bacteria fertilizer can secrete organic acid and phosphatase, organic acid mineral phosphorus can be converted into solvable dihydrogen phosphate and
Phosphoric acid hydrogen radical ion, this process are known as the degraded of mineral phosphorus；Phosphatase promotes the mineralising (hydrolysis) of phosphorus to be efficiently used by plant.
As it can be seen that molten bacterium is promoting plant phosphorus element absorption aspects to play an important role.P elements in soil are improved using microbial pathway
Validity, can increase the trophic level of soil soluble phosphorus, promote plant to efficiently use phosphorus, improve edaphon ring
Border.
Series bacillus has the characteristics that survival period length and resistance are strong, is widely distributed in different soil and plant rhizosphere
In, what is had can also invaded plants organization internal.Many bacterial strains in the category are with its fixed nitrogen, phosphate solubilization and to pathogenic bacteria
Resistance and growth-promoting functions to plant cause it to receive the extensive concern of researcher.
Microbial manure refers to containing microorganism living, and provides nutrition, tune to plant by the specific function of microorganism
Save the particular product of plant growth.Microbial manure is one of important directions of following agriculture and forestry sustainable development.But strain
Single, function is single and the distinct issues such as the shelf-life is short still have it is to be solved.
The content of the invention
In order to solve the problems in the existing technology, the object of the present invention is to provide the class bud that one plant has phosphate solubilization
Application of the microbial bacterial agent or bacterial manure of spore bacillus strain T1-1 and its preparation in vegetables etc. are planted.
In order to realize the object of the invention, technical scheme is as follows：
In a first aspect, the present invention provides the series bacillus bacterial strain T1-1 that one plant has phosphate solubilization, class is identified as
Bacillus (Paenibacillus sp.), is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center
(abbreviation CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode：
100101), preservation date is on November 17th, 2017, and deposit number is CGMCC No.14927.
Series bacillus bacterial strain T1-1 provided by the invention is good in LD cultured on solid medium, bacterium colony ecru, side
Edge is smooth not to have a gauffer, diameter 1mm-1.5mm, is gram-positive bacteria, 25-55 DEG C of growth temperature, 37 DEG C of optimum growth temp,
Optimal pH 6.8-7.0.Under bad condition, thalline conversion produces gemma, and gemma is strong to poor environment resistance, can the short time
It is resistant to 100 DEG C of high temperature.
In order to facilitate the dissolving P capacity of analysis bacterial strain, Design Laboratory phosphorus decomposing experimental study bacterial strain is to Phos and organophosphor
Dissolving, detailed in Example 2-3.
Second aspect, the present invention provides a kind of microbial bacterial agent or microorganism fertilizer containing series bacillus bacterial strain T1-1
Material.
Further, the microbial bacterial agent can be the zymotic fluid or nutrient solution of the series bacillus bacterial strain T1-1,
Specifically preparation method can be：
Series bacillus bacterial strain T1-1 is inoculated into fluid nutrient medium, 30 DEG C of incubator cultures, treats that 85% thalline produces
Stop culture after gemma, obtain zymotic fluid or nutrient solution.
Wherein, the formula of the fluid nutrient medium is：10g/L glucose, 5.0g/L Ca3PO4)2、5.0g/L MgCl2、
0.25g/L MgSO4·7H2O、0.2g/L KCl、1g/L(NH4)2SO4, pH 7.0.121 DEG C of sterilizing 25min.
When the series bacillus bacterial strain T1-1 needs to carry out solid culture, used solid medium can be foregoing
14g agar is added in every liter of fluid nutrient medium.
Further, the microbial manure contains aforementioned micro organism microbial inoculum and absorption carrier.
The absorption carrier is made of turfy soil, potassium humate, vermiculite and ground phosphate rock；Preferably, it is 3 according to mass ratio
~5：0.5~1.5：1~2：0.1~0.5 composition, more preferably according to mass ratio 3.5：1：1.5：0.3 composition.Absorption carrier system
When standby, 100 mesh sieves are crossed after taking above-mentioned raw materials co-grinding, are sterilized, cooling is spare.
Preferably, the microbial manure is 1 according to mass ratio by microbial bacterial agent and absorption carrier:1 ratio is mixed
After conjunction, drying forms.
Wherein, the microbial bacterial agent adjusts its bacterium colony concentration not less than 10 before being mixed with absorption carrier8CFU/mL。
In the specific embodiment of the present invention, when preparing microbial manure, used microbial bacterial agent is 5 × 108CFU/
mL。
The microbial manure can impose on plantation grass wheat, corn, rice, Curcurbitaceae cucumber etc..
The third aspect, the present invention provides applications of the series bacillus bacterial strain T1-1 in terms of phosphorus decomposing preparation is prepared.
And preparation applications agricultural production in terms of of the series bacillus bacterial strain T1-1 as active ingredient.
And the application of aforementioned micro organism microbial inoculum or microbial manure in vegetable cultivation.Microorganism fertilizer of the present invention
Expect that the growth to vegetables has significant facilitation, can effectively reduce the usage amount of chemical phosphatic ferfilizer, can be pushed away in agricultural production
It is wide to use.
After testing, series bacillus bacterial strain T1-1 provided by the present invention and the microbial bacterial agent especially prepared or microorganism
Bacterial manure stores nontoxic, no pathogenicity to people.
The present invention relates to raw material or reagent be ordinary commercial products, the operation being related to is unless otherwise specified
This area routine operation.
The beneficial effects of the present invention are：
Series bacillus bacterial strain T1-1 provided by the present invention be it is isolated from grass Rhizosphere of Crops soil,
It such as can produce gemma with stronger adaptive capacity to environment in uncomfortable environment.Meanwhile laboratory phosphorus decomposing experiment and plant
Result of the test shows that the bacterial strain has solvability to a variety of inorganic or organic phosphorus sources, it follows that the bacterial strain can be used for soil
The dissolving of middle phosphorus source.On the other hand, tested according to laboratory phosphorus decomposing, series bacillus bacterial strain T1-1 is in NBRIP fluid nutrient mediums
In it is good to phosphorus source solute effect, show that such Bacillus strain T1-1 can be used to dissolve the P elements in liquid environment, such as
The dissolving of phosphorus source in eutrophication water.
The present invention is used in soil with series bacillus bacterial strain T1-1 isolated in grass Rhizosphere of Crops soil
Inorganic, organic phosphorus sources dissolvings, have the characteristics that solve phosphorus stabilizer, efficiently, wide spectrum, and be conducive to plant absorption growth necessary to
P elements, promote the growing way of plant；The microbial manure being mixed to prepare with the bacterial strain fermentation liquor and fertilizer matrix (absorption carrier),
Dominant microflora can be quickly formed after being manured into soil, fertilizer efficiency is good, can significantly improve available phosphorus content in soil, promotes the strong of plant
Kang Shengchang.The bacterial strain is used for inorganic, organic phosphorus sources dissolvings in eutrophication water, water plant can be promoted to absorb more in water body
Remaining P elements, so as to prevent body eutrophication.
Embodiment
With reference to embodiment the present invention will be further explained explanation.It will be appreciated that following embodiments provide
Merely to playing the purpose of explanation, it is not used to limit the scope of the present invention.Those skilled in the art is not
In the case of spirit of the invention and spirit, various modifications and replacement can be carried out to the present invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples, is commercially available unless otherwise specified.
The preparation of 1 microbial bacterial agent of embodiment
Series bacillus bacterial strain T1-1 is inoculated into fluid nutrient medium, 30 DEG C of incubator cultures, treats that 85% thalline produces
Stop culture after gemma, obtain zymotic fluid or nutrient solution.
Wherein, the formula of the fluid nutrient medium is：10g/L glucose, 5.0g/L Ca3(PO4)2、5.0g/L MgCl2、
0.25g/L MgSO4·7H2O、0.2g/L KCl、1g/L(NH4)2SO4, pH 7.0.121 DEG C of sterilizing 25min.
In gained zymotic fluid, bacterium colony concentration is 5 × 108CFU/mL。
The measure of phosphorus solvability in 2 fluid nutrient medium of embodiment
100mL NBRIP fluid nutrient mediums (10g/L glucose, 5.0g/L MgCl are poured into 250mL conical flasks2、
0.25g/L MgSO4·7H2O、0.2g/L KCl、1g/L(NH4)2SO4, pH 7.0), it is separately added into 5.0g/L Phos Ca3
(PO4)2、FePO4、AlPO4And 2.0mL/L organophosphors (lecithin) are used as phosphorus source.
The zymotic fluid of the gained of embodiment 1 is inoculated with, to be inoculated with e. coli jm109 as control, shaking table culture (28 DEG C, 160r/
min)8d.(10 000r/min) 15min is centrifuged at 4 DEG C, takes suitable supernatant, measure phosphorus content (survey by molybdenum antimony resistance colorimetric method
It is fixed), pH value.
The results show that series bacillus bacterial strain T1-1 can dissolve different Phos and organophosphor, and to Ca3(PO4)2's
Phosphorus solvability is very strong, reaches 378mg/L.It also has organophosphor lecithin certain phosphorus solvability at the same time.
The measure of phosphorus solvability in 3 solid medium of embodiment
The phosphate solubilization of Soluble phosphorus series bacillus T1-1 is measured with NBRIP solid mediums.By Soluble phosphorus class gemma bar
Bacterium T1-1 activated strains in LD solid mediums, 30 DEG C are incubated overnight；By the single bacterium colony sterile toothpick activated containing
Ca3(PO4)2The centers of NBRIP solid mediums carry out pricking method inoculation, be then incubated 7 days at 30 DEG C, experiment is triplicate.Survey
Measure the colony diameter (d) and Soluble phosphorus loop diameter (D) of periphery of bacterial colonies.
Phosphorus solubilising index (PSI)：PSI=D/d.
The results show that series bacillus bacterial strain T1-1 is containing Ca3(PO4)2The PSI of solid NBRIP culture mediums be
2.46。
The preparation of 4 microbial manure of embodiment
1st, it is 3.5 according to mass ratio by turfy soil, potassium humate, vermiculite and ground phosphate rock：1：1.5：After 0.3 ratio mixing
Crush, cross 100 mesh sieves, sterilize, cooling, obtains microbial manure absorption carrier (matrix), spare；
2nd, by 1 gained zymotic fluid of embodiment and step 1 gained absorption carrier with mass ratio 1：1 mixing, drying, up to micro- life
Thing fertilizer.
The application effect of 5 microbial manure of embodiment
Wheat seed is put in after sterile-processed and is covered with the sterilized petri dishes of moistening filter paper, is urged as 25 DEG C of insulating boxs
Bud.Picking develops consistent seedling replanting into plastic tub after germination, per basin three seedling of kind.By microbial manure with base fertilizer
Form applies (0.4g/ basins), makees blank control using absorption carrier.Three repetitions of experimental setup.Destruction is used after transplanting second week
Property sampling method collect the root and cauline leaf of plant, record root long and stem length respectively.
The result shows that increase than blank control plant height and fresh weight respectively using microbial manure up to 35% and 54% (table 1),
As it can be seen that the microbial manure of the present invention has better plant growth-promoting effect, the usage amount of phosphate fertilizer can be effectively reduced.Cause
This, which has potential popularization market in the application of the plants such as vegetables, it is expected to obtains good economic benefit.
| Processing | Plant height (cm) | Fresh weight (g) |
| Control | 20.43b | 0.28b |
| Microbial manure | 27.59a | 0.43a |
Data carry out data analysis through statistic software SPSS in table, and the difference letter in same row is represented in p=0.05 water
Flat lower significant difference.
It should be appreciated that after the dosage to above-described embodiment agents useful for same or raw material carries out equal proportion expansion or reduces
Technical solution, it is substantially identical with above-described embodiment.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (9)
1. a kind of series bacillus bacterial strain T1-1 with phosphate solubilization, it is characterised in that its deposit number is CGMCC
No.14927。
A kind of 2. microbial bacterial agent containing bacterial strain described in claim 1.
3. microbial bacterial agent according to claim 2, it is characterised in that its preparation method is：By series bacillus bacterial strain
T1-1 is inoculated into fluid nutrient medium, 30 DEG C of incubator cultures, is stopped culture after 85% thalline produces gemma, is obtained zymotic fluid
Or nutrient solution；
Wherein, the formula of the fluid nutrient medium is：10g/L glucose, 5.0g/L Ca3(PO4)2、5.0g/L MgCl2、
0.25g/L MgSO4·7H2O、0.2g/L KCl、1g/L(NH4)2SO4, 7.0,121 DEG C of sterilizing 25min of pH.
A kind of 4. microbial manure containing bacterial strain described in claim 1.
5. microbial manure according to claim 4, it is characterised in that its preparation method is：By the bacterium colony of the bacterial strain
Number is not less than 108The microbial inoculum of CFU/mL is with absorption carrier with 1~3:After 1~3 mass ratio mixing, drying forms.
6. microbial manure according to claim 5, it is characterised in that the microbial inoculum is with absorption carrier with 1:1 quality
Than mixing.
7. the microbial manure according to claim 5 or 6, it is characterised in that the absorption carrier is by turfy soil, humic acid
Potassium, vermiculite and ground phosphate rock are 3~5 according to mass ratio：0.5~1.5：1~2：0.1~0.5 composition, preferably according to mass ratio
3.5：1：1.5：0.3 composition.
8. micro- life described in series bacillus bacterial strain T1-1 or its tunning or Claims 2 or 3 described in claim 1
The application of thing microbial inoculum or claim 4~7 any one of them microbial manure on crop yield and quality is improved.
9. micro- life described in series bacillus bacterial strain T1-1 or its tunning or Claims 2 or 3 described in claim 1
Thing microbial inoculum or claim 4~7 any one of them microbial manure answering in microbial manure or biological organic fertilizer is prepared
With.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711485168.6A CN108034609B (en) | 2017-12-29 | 2017-12-29 | Paenibacillus strain T1-1 with phosphate solubilizing effect and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108676744A (en) * | 2018-05-16 | 2018-10-19 | 南京农业大学 | A kind of bacillus megaterium bacterial strain ZT-P and its application |
| CN110684696A (en) * | 2019-11-11 | 2020-01-14 | 乔志伟 | Bacillus megaterium QZY-3 and application thereof |
| CN110684695A (en) * | 2019-11-11 | 2020-01-14 | 乔志伟 | Paenibacillus polymyxa QZY-1 and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676744A (en) * | 2018-05-16 | 2018-10-19 | 南京农业大学 | A kind of bacillus megaterium bacterial strain ZT-P and its application |
| CN108676744B (en) * | 2018-05-16 | 2020-07-28 | 南京农业大学 | Bacillus megaterium strain ZT-P and application thereof |
| CN110684696A (en) * | 2019-11-11 | 2020-01-14 | 乔志伟 | Bacillus megaterium QZY-3 and application thereof |
| CN110684695A (en) * | 2019-11-11 | 2020-01-14 | 乔志伟 | Paenibacillus polymyxa QZY-1 and application thereof |
| CN110684696B (en) * | 2019-11-11 | 2022-04-19 | 安顺学院 | Bacillus megaterium QZY-3 and application thereof |
| CN110684695B (en) * | 2019-11-11 | 2022-10-04 | 安顺学院 | Paenibacillus polymyxa QZY-1 and application thereof |
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| CN108034609B (en) | 2021-04-27 |
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