CN108017713A - One step membrane filter method separates the device and method of milk antibodies - Google Patents
One step membrane filter method separates the device and method of milk antibodies Download PDFInfo
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- CN108017713A CN108017713A CN201610964513.3A CN201610964513A CN108017713A CN 108017713 A CN108017713 A CN 108017713A CN 201610964513 A CN201610964513 A CN 201610964513A CN 108017713 A CN108017713 A CN 108017713A
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- 239000012528 membrane Substances 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 37
- 235000013336 milk Nutrition 0.000 title claims abstract description 12
- 239000008267 milk Substances 0.000 title claims abstract description 12
- 210000004080 milk Anatomy 0.000 title claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 46
- 239000000919 ceramic Substances 0.000 claims abstract description 43
- 238000011118 depth filtration Methods 0.000 claims abstract description 37
- 235000020247 cow milk Nutrition 0.000 claims abstract description 36
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 27
- 150000002632 lipids Chemical class 0.000 claims abstract description 7
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 5
- 238000005374 membrane filtration Methods 0.000 claims abstract description 4
- 230000000694 effects Effects 0.000 claims description 21
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 15
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 230000007704 transition Effects 0.000 claims description 10
- 238000010521 absorption reaction Methods 0.000 claims description 7
- 230000000740 bleeding effect Effects 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 229910010293 ceramic material Inorganic materials 0.000 claims description 5
- RVTZCBVAJQQJTK-UHFFFAOYSA-N oxygen(2-);zirconium(4+) Chemical compound [O-2].[O-2].[Zr+4] RVTZCBVAJQQJTK-UHFFFAOYSA-N 0.000 claims description 5
- 239000000377 silicon dioxide Substances 0.000 claims description 5
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 claims description 5
- 229910001928 zirconium oxide Inorganic materials 0.000 claims description 5
- 229910016341 Al2O3 ZrO2 Inorganic materials 0.000 claims description 4
- 239000002131 composite material Substances 0.000 claims description 4
- 230000035699 permeability Effects 0.000 claims description 4
- 239000002893 slag Substances 0.000 claims description 4
- 239000002158 endotoxin Substances 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 abstract description 13
- 238000004587 chromatography analysis Methods 0.000 abstract description 10
- 229960000074 biopharmaceutical Drugs 0.000 abstract description 5
- 239000005862 Whey Substances 0.000 abstract description 4
- 102000007544 Whey Proteins Human genes 0.000 abstract description 4
- 108010046377 Whey Proteins Proteins 0.000 abstract description 4
- 239000013618 particulate matter Substances 0.000 abstract description 4
- 239000000463 material Substances 0.000 description 8
- 238000013461 design Methods 0.000 description 5
- 239000010451 perlite Substances 0.000 description 4
- 235000019362 perlite Nutrition 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000012501 chromatography medium Substances 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000004743 Polypropylene Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000009881 electrostatic interaction Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/04—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from milk
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Analytical Chemistry (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The present invention provides the device and method of step membrane filter method separation milk antibodies, wherein, method includes：Step 1：The volume that 2% is pressed in cow's milk is added into the other diatomite of pharmaceutical grade；Step 2：For cow's milk after being mixed by peristaltic pump into filter, cow's milk first passes through ceramic membrane, and lipid in cow's milk is separated；Step 3：Remainder passes through in-depth filtration membrane filtration.Using the above scheme, incorporate the in-depth filtration of traditional bio-pharmaceutical industry and the technology of chromatography, form the closed system for meeting GMP pharmaceutical standards, ceramic membrane can separate the lipid component in cow's milk, separation can adsorb large particulate matter by diatomite filtering at the same time, and then the whey clarified can be carried out the chromatography of next step, its time is that traditional first filter chromatographs the 1/2 of technique afterwards, economic direct cost is saved about more than three times, while improves Product Safety.
Description
Technical field
The invention belongs to life science and field of biological pharmacy technical field, more particularly to a step membrane filter method
Separate the device and method of milk antibodies.
Background technology
Life science and the industrialization product of field of biological pharmacy ferment greatly mostly from reactor at present, but also have one
Portioned product comes from animal sources, and the albumen or antibody of animal sources are, it is necessary to the means of transgenosis by humanized proteins or antibody
Expressed by animal such as ox, the expression cycle is short, and expression yield is high, and relatively low expression environmental requirement is all such technology
Advantage, but inferior position is that the foreign protein/miscellaneous antibody expressed is more, and cow's milk impurity is more, and milk elements caused by the reason such as environment have shakiness
It is qualitative, if using disk plate centrifuge, ceramic membrane, rotor centrifugation, chromatographed again after three-step approach by the way of obtain antibody, problem
It is that 1. efficiency are low, feed liquid particle is bigger than normal after excessive 2. 3 step of time cost, so, chromatography media will block during chromatography several times,
Chromatography media is thus needed replacing, cost is high, and risk is also very big.
Therefore, the prior art is defective, it is necessary to improve.
The content of the invention
The technical problems to be solved by the invention are in view of the deficiencies of the prior art, there is provided a step membrane filter method separates cow's milk
The device and method of antibody.
The present invention seeks to one-step method by cow's milk liquid high efficiency filter, and not only good filtration effect also reaches repeatedly chromatography without stifled
Chromatography media acts on purpose, can so substantially reduce business economic cost and time cost, while also reduce process risk.
The present invention provides a kind of method of step membrane filter method separation milk antibodies, wherein, comprise the following steps：
Step 1：The volume that 2% is pressed in cow's milk is added into the other diatomite of pharmaceutical grade；
Step 2：For cow's milk after being mixed by peristaltic pump into filter, cow's milk first passes through ceramic membrane, by fat in cow's milk
Class is separated；
Step 3：Remainder passes through in-depth filtration membrane filtration.
The method, wherein, before step 1, the other diatomite of pharmaceutical grade will be added in 2% ratio in cow's milk.
The method, wherein, the in-depth filtration film uses 0.45um apertures.
The method, wherein, the rate of filtration filtered described in step 3 is 10ml/min/cm2。
The present invention also provides a kind of device of step membrane filter method separation milk antibodies, including filter entrance and filter out
Mouthful, wherein, further include the ceramic membrane entrance being connected with filtering entrance and inlet tube, inlet tube are connected with filter housing, the entrance
Ceramic membrane and permeability hole, the air bleeding valve set on filter housing are provided with pipe；The filter housing is connected with in-depth filtration film, deep layer mistake
Filter membrane is arranged in bottom support plate, and the positive centre of bottom support plate is additionally provided with filtering outlet.
The device, wherein, the air bleeding valve is additionally provided with pressure gauge, for control pressure；The filtering outlet is set
Put and be connected with disposably harvest liquid storing bag.
The device, wherein, the ceramic membrane plays the role of coarse filtration；The in-depth filtration film is that fine filtering is made
With；In-depth filtration film is clarified by what lignocellulosic, perlite formed with that can remove the effect of absorption bulky grain, at the same time also
There are absorption endotoxin, the effect of host's foreign protein.
The device, wherein, the ceramic membrane is porous by inorganic ceramic materials such as aluminium oxide, zirconium oxide, titanium oxide
Supporting layer, transition zone and separating layer composition；Transition zone composition is silica；The thickness of the ceramic film is 50-60 μm, pottery
The aperture of porcelain film is 0.01-0.5 μm；The porosity：44-46%；Filter pressure：0.15Mpa, kickback pressure：Below 0.7Mpa；
Ceramic membrane is duplicature, outer membrane TiO2；Inner membrance is Al2O3-ZrO2 composite membranes.
The device, wherein, the aperture of the in-depth filtration film is 35um to 0.04um；Cardboard slag amount is 4kg/
m2。
Using the above scheme, the in-depth filtration of traditional bio-pharmaceutical industry and the technology of chromatography are incorporated, formation meets
The closed system of GMP pharmaceutical standards, ceramic membrane can separate the lipid component in cow's milk, and separation is filtered by diatomite at the same time
Large particulate matter can be adsorbed, and then the whey clarified can be carried out the chromatography of next step, its time is that tradition first filters
The 1/2 of technique is chromatographed afterwards, and economic direct cost is saved about more than three times, while improves Product Safety.
Brief description of the drawings
Fig. 1 is the structure diagram of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
Embodiment 1
Currently on the market filter a certain general film product of class, be all only filtration, but for a 0.45um,
The film of 20cm2, the filtration yield for the treatment of of cow's milk only have 2ml or so, cannot meet the needs of scientific research and enterprise's filtering, Wu Fajin completely
Row industrialization amplification, time cost, financial cost are excessive, and it is big that a kind for the treatment of capacity urgently occurs in the market, and can integrate several steps
The filter of clarifying effect come complete a step filtering clarifying effect excellent product.
As shown in Figure 1, the constituent of ceramic membrane 8 includes：The inorganic ceramic materials such as aluminium oxide, zirconium oxide, titanium oxide are more
Hole supporting layer, transition zone and separating layer；Wherein the component of transition zone is silica；Separation composition of layer is poly- third film of micro-filtration rank；
5 constituent of in-depth filtration film includes：The composition of the polypropylene and filter board, wherein filter board of supporting role
Component lignocellulosic, perlite and diatomite；Wherein diatomaceous constituent is amorphous silica.
Connection relation：Ceramic membrane 8 is placed in the top of filter housing 4, then in-depth filtration film 5 is placed in 4 bottom of filter housing, in filter housing 4
Portion leaves certain filtering cow's milk space, and closed continuous filtration system is made with the polypropylene material of supporting role.
The operation principle of apparatus of the present invention：The in-depth filtration of traditional bio-pharmaceutical industry and the technology of chromatography are incorporated,
The closed system for meeting GMP pharmaceutical standards is formed, ceramic membrane can separate the lipid component in cow's milk, and separation passes through silicon at the same time
Diatomaceous earth filtering can adsorb large particulate matter, and then the whey clarified can be carried out the chromatography of next step, its time is to pass
System first filters chromatographs the 1/2 of technique afterwards, and economic direct cost saving reaches more than three times, while improves Product Safety.
Furthermore, the method the present invention also provides step membrane filter method separation milk antibodies is：2% will be pressed in cow's milk
Volume add the other diatomite of pharmaceutical grade, for the cow's milk after being mixed by peristaltic pump into filter, cow's milk first passes through ceramics
Film, lipid in cow's milk is separated, and at the same time remainder passes through in-depth filtration film, this film can retain many miscellaneous big
Particle, since film uses 0.45um apertures, therefore obtained clarified solution effect is fine, can reach and do the standard chromatographed in next step,
One-step method completes thorough filter effect, and operation very easily and avoids cross contamination, and is easy to amplify, particularly with type of production
Enterprise, can greatly save time, financial cost, play the role of important.
Ceramic membrane：Ceramic membrane is by the inorganic ceramic material such as aluminium oxide, zirconium oxide, titanium oxide porous support layer, transition zone
And separating layer composition, its transition zone is silica；Ceramic membrane is that a kind of have the function of the inorganic or high score of special Selective Separation
Sub- material, it can enable one or more of materials therein to pass through fluid partitioning into two parts being not communicated with, and will be other
Material is separated.Membrane separation technique has been widely used in doctor with the characteristic such as its efficient, energy-saving and environmental protection and molecular level filtering
The fields such as medicine, water process, chemical industry, electronics, food processing, become one of most important technology in this century separation science, are recognized
It is a kind of emerging green industry science and technology for the membrane technology of one of 21 century most great industrial technology.Thicknesses of layers：50—60μ
M, 0.01-0.5 μm of membrane aperture；The porosity：44-46%；Filter pressure：0.15Mpa, kickback pressure：Below 0.7Mpa；Membrane material
Matter：Duplicature, outer membrane TiO2；Inner membrance Al2O3-ZrO2 composite membranes.
In-depth filtration film 5 clamps setting together by filter housing 4 and bottom support plate 6, ensures the fixed position of in-depth filtration film 5
It will not move, and bottom support plate 6 has supporting role to in-depth filtration film 5.Various sizes can be made in in-depth filtration film 5
And shape, including square, circle, rectangle, perforate/non-porous, folding etc..Cardboard can be generally divided into from 35um by aperture point
To a variety of grades of 0.04um, aperture is smaller, and separating effect is more clarified；Cardboard aperture may diminish to filter out bacterium (bacterial index
Up to LRV8), produce sterile product.Cardboard slag amount is also very high, generally up to 4kg/m2.In filter process, flow velocity drop
Low solid particle, finally retains by the labyrinth passage inside cardboard or under electrostatic interaction and clarifies.Have benefited from this only
Special mechanism, cardboard filter amount are larger (until channel jam, cardboard long lifespan).
As shown in Figure 1, the structure of the present invention includes：Retain and filter common general filtering entrance 1, filtering outlet 10,
Scientific research and enterprise's use habit are not changed.Filter housing 4 in Fig. 1, are the filter housings 4 that can hold 200ml solvents, and so design can improve
Speed and filtration yield, and be easy to amplification and make, so as to meet life science scientific research and Biopharmaceutical Enterprises demand very well.Figure
Air bleeding valve 3 in 1, are scavenging actions, and so design can relieve stress, and can also place pressure gauge here, play control pressure
Power acts on, and so design is also consistent with enterprise scale up test needs, safely, meets GMP needs.Filtering outlet 10, can be direct
It is connected with disposable harvest liquid storing bag, largely avoided the probability of pollution, also help the purification process of next step.In Fig. 1
Ceramic membrane 8 plays the role of coarse filtration, and in-depth filtration film 5 is fine filtering effect.When the solution such as cow's milk liquid of filtering first adds medicine
The amount of the diatomite 2% of thing rank, then during by ceramic membrane 8, the ceramic membrane entrance 7 of ceramic membrane 8 is first entered from filtering entrance 1,
After ceramic membrane filter can be come out from permeability hole 9, into filter housing 4, and then touch in-depth filtration film 5, in-depth filtration film 5 be by
Lignocellulosic, having for perlite composition can remove the effect of absorption bulky grain and clarify, while also have absorption endotoxin, host
Foreign protein etc. acts on, and object will be flowed through out and be collected into from Fig. 1 at filtering outlet 10, this design plays step reality
Cow's milk filter effect is now clarified, it is quick and efficient.
Since the space of filter housing 4 in Fig. 1 is larger, it is other pharmaceutical grade can will to be added in 2% ratio in cow's milk before filtration
Diatomite, play the role of increase filtration treatment amount, spatial design it is bigger, treating capacity is bigger, it is contemplated that practical application with
Enterprise needs, temporarily using the pre- allowance of 200ml or so；And the increasing of filtration treatment amount, treating capacity itself can be big with ceramic membrane 8
Match, so, ceramic membrane 8, in-depth filtration film 5 has each played maximum effect effect, hence for scientific research and enterprise
It is greatly convenient and safe that industry filtering cow's milk wishes that the effect that a step is clarified has.
Optimal use state：According to material liquid volume, the diatomite of 2% volume, rate of filtration 10ml/ are added in feed liquid first
Min/cm2 handles feed liquid, and clarification filtration effect is realized by ceramic membrane and one step of in-depth filtration film.
Embodiment 2
On the basis of above-described embodiment, furthermore, a step membrane filter method separates the method for milk antibodies, its feature
It is, comprises the following steps：
Step 1：The volume that 2% is pressed in cow's milk is added into the other diatomite of pharmaceutical grade；
Step 2：For cow's milk after being mixed by peristaltic pump into filter, cow's milk first passes through ceramic membrane, by fat in cow's milk
Class is separated；
Step 3：Remainder passes through in-depth filtration membrane filtration.
Before step 1, the other diatomite of pharmaceutical grade will be added in 2% ratio in cow's milk；The in-depth filtration film uses
0.45um apertures；The rate of filtration filtered described in step 3 is 10ml/min/cm2。
The present invention on the basis of the above, also provides the device of step membrane filter method separation milk antibodies, such as Fig. 1 institutes
Show, including filtering entrance 1 and filtering outlet 10, it is characterised in that further include with filter ceramic membrane entrance 7 that entrance 1 is connected and
Inlet tube 2, inlet tube 2 are connected with filter housing 4, and ceramic membrane 8 and permeability hole 9 are provided with the inlet tube 2, is set on filter housing 4
Air bleeding valve 3；The filter housing 4 is connected with in-depth filtration film 5, and in-depth filtration film 5 is arranged in bottom support plate 6, bottom branch
The positive centre of fagging 6 is additionally provided with filtering outlet 10.Bottom support plate 6 meets the plate of the plastic material of GMP requirements；Specific effect is just
It is support.The air bleeding valve 3 is additionally provided with pressure gauge, for control pressure；The filtering outlet 10 is set to be received with disposable
Liquid storing bag is obtained to be connected；The ceramic membrane 8 plays the role of coarse filtration；The in-depth filtration film 5 is fine filtering effect；In-depth filtration
Film 5 is clarified by what lignocellulosic, perlite formed with that can remove the effect of absorption bulky grain, while also has absorption endogenous toxic material
Element, the effect of host's foreign protein.The ceramic membrane be by the inorganic ceramic material such as aluminium oxide, zirconium oxide, titanium oxide porous support layer,
Transition zone and separating layer composition；Transition zone composition is silica；The thickness of the ceramic film is 50-60 μm, the hole of ceramic membrane
Footpath is 0.01-0.5 μm；The porosity：44-46%；Filter pressure：0.15Mpa, kickback pressure：Below 0.7Mpa；Ceramic membrane is
Duplicature, outer membrane TiO2；Inner membrance is Al2O3-ZrO2 composite membranes.The aperture of the in-depth filtration film is 35um to 0.04um；
Cardboard slag amount is 4kg/m2.
Using the above scheme, the in-depth filtration of traditional bio-pharmaceutical industry and the technology of chromatography are incorporated, formation meets
The closed system of GMP pharmaceutical standards, ceramic membrane can separate the lipid component in cow's milk, and separation is filtered by diatomite at the same time
Large particulate matter can be adsorbed, and then the whey clarified can be carried out the chromatography of next step, its time is that tradition first filters
The 1/2 of technique is chromatographed afterwards, and economic direct cost is saved about more than three times, while improves Product Safety.
It should be appreciated that for those of ordinary skills, can according to the above description be improved or converted,
And all these modifications and variations should all belong to the protection domain of appended claims of the present invention.
Claims (9)
1. the method that a step membrane filter method separates milk antibodies, it is characterised in that comprise the following steps：
Step 1：The volume that 2% is pressed in cow's milk is added into the other diatomite of pharmaceutical grade；
Step 2：For cow's milk after being mixed by peristaltic pump into filter, cow's milk first passes through ceramic membrane, by lipid in cow's milk point
Separate out；
Step 3：Remainder passes through in-depth filtration membrane filtration.
2. the method as described in claim 1, it is characterised in that before step 1, medicinal by being added in cow's milk in 2% ratio
The diatomite of rank.
3. the method as described in claim 1, it is characterised in that the in-depth filtration film uses 0.45um apertures.
4. the method as described in claim 1, it is characterised in that the rate of filtration filtered described in step 3 is 10ml/min/
cm2。
5. a step membrane filter method separates the device of milk antibodies, including filtering entrance and filtering outlet, it is characterised in that further includes
The ceramic membrane entrance and inlet tube, inlet tube being connected with filtering entrance are connected with filter housing, and ceramics are provided with the inlet tube
Film and permeability hole, the air bleeding valve set on filter housing；The filter housing is connected with in-depth filtration film, and in-depth filtration film is arranged on bottom
In support plate, the positive centre of bottom support plate is additionally provided with filtering outlet.
6. device as claimed in claim 5, it is characterised in that the air bleeding valve is additionally provided with pressure gauge, for control pressure；
The filtering outlet is set to be connected with disposable harvest liquid storing bag.
7. device as claimed in claim 5, it is characterised in that the ceramic membrane plays the role of coarse filtration；The in-depth filtration
Film is fine filtering effect；In-depth filtration film by lignocellulosic, perlite form have can remove adsorb bulky grain work
With and clarify, while also have absorption endotoxin, host's foreign protein effect.
8. device as claimed in claim 5, it is characterised in that the ceramic membrane is by nothings such as aluminium oxide, zirconium oxide, titanium oxide
Machine ceramic material porous support layer, transition zone and separating layer composition；Transition zone composition is silica；The thickness of the ceramic film
For 50-60 μm, the aperture of ceramic membrane is 0.01-0.5 μm；The porosity：44-46%；Filter pressure：0.15Mpa, recoil pressure
Power：Below 0.7Mpa；Ceramic membrane is duplicature, outer membrane TiO2；Inner membrance is Al2O3-ZrO2 composite membranes.
9. device as claimed in claim 5, it is characterised in that the aperture of the in-depth filtration film is 35um to 0.04um；Paper
Plate slag amount is 4kg/m2.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108593404A (en) * | 2018-07-06 | 2018-09-28 | 无锡宏众基因科技有限公司 | A kind of screw push type sample extraction device |
| CN111440226A (en) * | 2020-05-27 | 2020-07-24 | 杭州奕安济世生物药业有限公司 | System and method for protein purification |
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