CN107969441A - Tobacco extract is used for the purposes of citrus fruit fly insecticide - Google Patents
Tobacco extract is used for the purposes of citrus fruit fly insecticide Download PDFInfo
- Publication number
- CN107969441A CN107969441A CN201711249501.3A CN201711249501A CN107969441A CN 107969441 A CN107969441 A CN 107969441A CN 201711249501 A CN201711249501 A CN 201711249501A CN 107969441 A CN107969441 A CN 107969441A
- Authority
- CN
- China
- Prior art keywords
- citrus fruit
- fruit fly
- tobacco
- insecticide
- tobacco extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 83
- 239000000284 extract Substances 0.000 title claims abstract description 51
- 241000255588 Tephritidae Species 0.000 title claims abstract description 50
- 235000020971 citrus fruits Nutrition 0.000 title claims abstract description 50
- 239000002917 insecticide Substances 0.000 title claims abstract description 30
- 244000061176 Nicotiana tabacum Species 0.000 title 1
- 241000208125 Nicotiana Species 0.000 claims abstract description 83
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000007921 spray Substances 0.000 claims description 6
- 239000003495 polar organic solvent Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 235000019504 cigarettes Nutrition 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 239000000575 pesticide Substances 0.000 abstract description 13
- 241000607479 Yersinia pestis Species 0.000 abstract description 12
- 239000003814 drug Substances 0.000 abstract description 6
- 229940079593 drug Drugs 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 4
- 230000009471 action Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 239000000447 pesticide residue Substances 0.000 abstract description 2
- 230000003449 preventive effect Effects 0.000 abstract description 2
- 230000000749 insecticidal effect Effects 0.000 description 14
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 12
- 229960002715 nicotine Drugs 0.000 description 12
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 10
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229930013930 alkaloid Natural products 0.000 description 3
- -1 for a long time Substances 0.000 description 3
- 230000002147 killing effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241000255967 Helicoverpa zea Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000256247 Spodoptera exigua Species 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 230000006652 catabolic pathway Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- MTXSIJUGVMTTMU-JTQLQIEISA-N (S)-anabasine Chemical compound N1CCCC[C@H]1C1=CC=CN=C1 MTXSIJUGVMTTMU-JTQLQIEISA-N 0.000 description 1
- MYKUKUCHPMASKF-VIFPVBQESA-N (S)-nornicotine Chemical compound C1CCN[C@@H]1C1=CC=CN=C1 MYKUKUCHPMASKF-VIFPVBQESA-N 0.000 description 1
- SOPPBXUYQGUQHE-JTQLQIEISA-N Anatabine Chemical compound C1C=CCN[C@@H]1C1=CC=CN=C1 SOPPBXUYQGUQHE-JTQLQIEISA-N 0.000 description 1
- SOPPBXUYQGUQHE-UHFFFAOYSA-N Anatabine Natural products C1C=CCNC1C1=CC=CN=C1 SOPPBXUYQGUQHE-UHFFFAOYSA-N 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 241000675108 Citrus tangerina Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 240000000233 Melia azedarach Species 0.000 description 1
- MYKUKUCHPMASKF-UHFFFAOYSA-N Nornicotine Natural products C1CCNC1C1=CC=CN=C1 MYKUKUCHPMASKF-UHFFFAOYSA-N 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 241000508269 Psidium Species 0.000 description 1
- 208000005392 Spasm Diseases 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical group CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 1
- 230000002164 acetylcholinergic effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 229930014345 anabasine Natural products 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 239000013530 defoamer Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 210000000087 hemolymph Anatomy 0.000 description 1
- 238000000589 high-performance liquid chromatography-mass spectrometry Methods 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 231100000636 lethal dose Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 229940080817 rotenone Drugs 0.000 description 1
- JUVIOZPCNVVQFO-UHFFFAOYSA-N rotenone Natural products O1C2=C3CC(C(C)=C)OC3=CC=C2C(=O)C2C1COC1=C2C=C(OC)C(OC)=C1 JUVIOZPCNVVQFO-UHFFFAOYSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 210000000225 synapse Anatomy 0.000 description 1
- 239000006273 synthetic pesticide Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
- A01N65/385—Tobacco
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the purposes that tobacco extract is used for citrus fruit fly insecticide, belong to pesticide field.The invention also discloses a kind of citrus fruit fly insecticide and disinsection method, the tobacco extract containing 0.8 1.4mg/ml in the insecticide.The present invention with tobacco extract replaces chemical pesticide control citrus fruit fly, not only has the advantages that preventive effect is high, low etc. without pesticide residue, target organisms high selectivity and the disease pest resistance to the action of a drug, while applies for the reasonable development of waste tobacco leaf and provide a new approach.
Description
Technical field
The invention belongs to pesticide field, and in particular to tobacco extract is used for the purposes of citrus fruit fly insecticide, the present invention
Further relate to a kind of citrus fruit fly insecticide and citrus fruit fly disinsection method.
Background technology
Tobacco is a kind of important industrial crops, wherein have more than 20% tobacco leaf can not use in process and by
It is discarded, so it is both the waste of resource, also pollutes the environment.Contain many valuable natural active matters in tobacco leaf, such as very
Already it is found to can be used as the nicotine of insecticide.In field of agricultural pesticide, for a long time, chemical synthetic pesticide by cheap and
The advantage of high activity occupies dominant position always, but chemical pesticide long-time service bring pesticide residue, target organisms selection
Property low and disease pest resistance problems it is more and more prominent, then people start to turn to sight into the botanical pesticide of natural reproducible.
Compared to traditional chemical pesticide, botanical pesticide source with R＆D costs it is low, be not likely to produce the resistance to the action of a drug, it is free from environmental pollution, with
Independent degradation pathway, it is safe to people, animal the features such as, common botanical pesticide has nicotine, print chinaberry, Dalmatian chrysanthemum, rotenone etc..
The material with insecticidal activity is mainly that nicotine, anatabine, nornicotine, anabasine etc. are raw in tobacco extract
Alkaloids, wherein nicotine content occupy the overwhelming majority of alkaloid.Nicotine is a kind of never poison, it can be to pass through insect bodies
Wall enters hemolymph, and a part of nicotine ion is then dissociated through nerve cell into synapses, the nicotine ion of generation
It is closely similar with acetylcholine structure, therefore nicotine has extremely strong affinity with acetylcholinergic receptor, nicotine acts on insect god
Through the acetylcholine nicotine receptor on cell, make neuron soma sustained activation, polypide continues spasm, and paralysis is dead.
Citrus fruit fly Bactracera dorsalis (Hendel) are worldwide quarantine pest insects, its host range is wide, breeding
Ability is strong, and can cause harm 46 section's planting fruit-trees more than 250 such as guava, mango, vegetables and flowers, is a kind of crushing pest, and tangerine is small
Trypetid is caused harm caused by fruits and vegetables to be mainly shown as that its larva is dived and occupies fruit flesh feeding, makes fruit rot, shedding.At present also
Have no the related report using tobacco extract prevention citrus fruit fly.
The content of the invention
It is an object of the present invention to provide the new application that a kind of tobacco extract is used for citrus fruit fly insecticide.
Insecticidal activity result of the test shows, flue-cured tobacco, burley tobaccos, Sun cured tobacco three grow tobacco extract have to citrus fruit fly compared with
The active highest of good insecticidal activity, wherein flue-cured tobacco；Tobacco extract to the insecticidal activity of citrus fruit fly there are dosage correlation,
There is preferable activity when concentration is higher than 0.8mg/ml；But to pests such as beet armyworm, bollworms in primary dcreening operation concentration range
Then has no activity.
Result above shows that tobacco extract is as a kind of botanical pesticide, available for prevention and control citrus fruit fly.
Heretofore described tobacco extract is prepared as follows：Tobacco is first extracted with water, then will be carried
Liquid is taken to be extracted with adjusting PH with base to 10-14 and with non-polar organic solvent, last extract concentration, be drying to obtain.
The non-polar organic solvent includes but not limited to chloroform, petroleum ether, benzene, ethyl acetate.
It is a further object to provide a kind of citrus fruit fly insecticide, the tobacco that it contains 0.8-1.4mg/ml carries
Take thing.
The citrus fruit fly insecticide can be the agricultures such as missible oil, aqueous suspension agent, oil-suspending agent, wettable powder, granule
Acceptable any type formulation on medicine.When preparing above-mentioned formulation, wetting agent can also be added into tobacco extract, is disperseed
Common auxiliary agent in the pesticides such as agent, thickener, antifreezing agent, disintegrant, binding agent, defoamer, carrier or filler.
The present invention further provides a kind of citrus fruit fly disinsection method, it includes the sprinkling citrus fruit fly insecticide
The step of.
According to one embodiment of present invention, in spraying insecticide, spray and contain when citrus fruit fly sprouts wings 1 week -3 week
1.2-1.4mg/mL the insecticide of tobacco extract；Sprinkling contains 0.8-1.0mg/mL tobacco extracts during 3 weeks -4 weeks of emergence
Insecticide；Emergence sprays the insecticide containing 1.0-1.2mg/mL tobacco extracts during 4 weeks -5 weeks, can farthest kill
Citrus fruit fly simultaneously reduces cost.
The beneficial effects of the invention are as follows：
(1) present invention replaces chemical pesticide control citrus fruit fly with tobacco extract, not only with preventive effect height, without pesticide
Residual, target organisms high selectivity and the advantages such as the disease pest resistance to the action of a drug is low, and tobacco extract has independent degradation pathway, will not
Environment is polluted, to people, animal safety.
(2) present invention is conducive to the recycling of waste tobacco leaf (flue-cured tobacco, burley tobaccos, Sun cured tobacco), is the conjunction of waste tobacco leaf
Manage development and application and one new approach is provided.
(3) extraction process is simple, and cost is low, it is easy to accomplish industrialized production.
Brief description of the drawings
Fig. 1 is the LC that flue-cured tobacco extract is administered different emergence period citrus fruit flies after 24h50Value.
Specific implementation method
The present invention is described in further detail with reference to example.
The preparation of 1 nicotiana alkaloids of embodiment
Dry tobacco leaf (flue-cured tobacco, burley tobaccos or Sun cured tobacco) is crushed with airslide disintegrating mill to obtain tobacco powder, takes 50g cigarettes
End adds 600mL tap water, mechanical agitation 5h under 60 DEG C of waters bath with thermostatic control, uses centrifuge in 1000mL three-necked flasks
(8000r/s, 4 DEG C, 15min), by extracting solution 50%NaOH solution tune pH to 13 or so, then 3 times are extracted with chloroform (every time
300mL), merge chloroform layer, be concentrated under reduced pressure into Rotary Evaporators dry, it is spare to be stored in 4 DEG C of refrigerators.The extract of three kinds of tobacco leaves
Recovery rate and nicotine content (being measured by HPLC-MS) see the table below 1.
The yield and nicotine content of 1 variety classes tobacco extract of table
| Tobacco varieties | Extract recovery rate (%) | Nicotine content (%) |
| Flue-cured tobacco | 2.85 | 61.06 |
| Burley tobaccos | 2.44 | 44.37 |
| Sun cured tobacco | 3.36 | 57.05 |
Insecticidal activity of 2 tobacco extract of embodiment to citrus fruit fly
(1) insect killing effect test of the various concentrations insecticide to citrus fruit fly
By flue-cured tobacco extract with a small amount of acetone solution, then with aqueous sucrose solution (w:V=1:10) various concentrations are diluted to
Liquid, liquid is uniformly soaked on smooth circular absorbent cotton (3g ± 0.1g) and is laid in 250ml wide mouthed Erlenmeyer bottom of bottle portion,
Sealed with individual layer white yarn cloth.By the citrus fruit fly test worm starvation 6h of 3 weeks after emergence, it is then added in above-mentioned conical flask, often
20, bottle, each concentration does 3 repetitions, using the sucrose water containing equivalent acetone as blank control group, is placed in bioassay room (T
=(28 ± 1) DEG C, RH=(75 ± 10) %, L: D=14h:Culture in 10h), counts test worm death condition after 24h, and with following
Formula calculates the test worm death rate.
The death rate=death number/test worm total number * 100%
Corrected mortality=(the treatment group death rate-control group death rate)/(the 1- control groups death rate) * 100%
Citrus fruit fly is administered the corrected mortality after 24h in 2 various concentrations flue-cured tobacco extract of table
As can be seen from the above table, tobacco extract and works as desinsection to the insecticidal activity of citrus fruit fly there are dosage correlation
When agent concentration reaches 0.8mg/mL, the citrus fruit fly death rate increases sharply, and when concentration is higher than 0.8mg/mL, the death rate continues to increase
The amplitude added is little, from insecticidal effect and the angle for reducing cost, selects insecticide concentration to be preferred for 0.8-1.4mg/mL.
(2) insect killing effect test of the insecticide to variety classes pest
According to the method in (1), lethal work of the flue-cured tobacco extract of 0.5mg/ml concentration to different pests administration 24h is detected
Property, the result is shown in table 3 below.
The death rate of 3 insecticide of table to different pests
As can be seen from the above table, insecticide has higher insecticidal activity to citrus fruit fly, and to beet armyworm, bollworm
Deng pest under primary dcreening operation concentration, without insecticidal activity.
(3) variety classes tobacco extract tests the insecticidal activity of citrus fruit fly
According to the method in (1), flue-cured tobacco, burley tobaccos, the Sun cured tobacco three of detection 0.5mg/mL concentration grow tobacco extract pair
The killing activity of 3-4 weeks citrus fruit fly of emergence, the result is shown in table 4 below.
Table 4 three grows tobacco the death rate of the extract to citrus fruit fly
| Extract species | Insecticide concentration | Corrected mortality |
| Flue-cured tobacco extract | 0.5mg/mL | 45.6% |
| Burley tobaccos extract | 0.5mg/mL | 38.6% |
| Sun cured tobacco extract | 0.5mg/mL | 39.5% |
As can be seen from the above table, three extracts that grow tobacco have citrus fruit fly preferable insecticidal activity, and wherein flue-cured tobacco carries
The active highest of thing is taken, Sun cured tobacco is lower slightly, and burley tobaccos activity is minimum.
(4) tobacco extract tests the insecticidal activity of different times citrus fruit fly
In order to study insecticidal activity of the tobacco extract to different times citrus fruit fly, we have chosen citrus fruit fly emergence
Different time, that is, sprout wings 1 week, 2 weeks, 3 weeks, 4 weeks, the citrus fruit fly of 5 weeks as test worm, it is dense that flue-cured tobacco extract is configured to difference
Degree, tests the death rate after different emergence period citrus fruit fly dispenser 24h, and uses 22 softwares of SPSS Statistics into line number
According to handling and calculate LC50(half lethal concentration).The result is shown in Figure 1.
It will be seen from figure 1 that flue-cured tobacco extract has differences the citrus fruit fly inhibitory activity of different emergence periods, after administration
LC50It is：Sprout wings the 2nd week>Sprout wings the 5th week>Sprout wings the 1st week>Sprout wings the 3rd week>Sprout wings the 4th week, extract is to citrus fruit fly
Insecticidal activity is most weak at the 2nd week, the 4th week highest.Such activity data can instruct our science when preventing citrus fruit fly
Medication, the 2nd week after sprouting wings early stage insect pest occurs (when generation overlap is less serious) when, increase dosage, in addition, to the 4th
When all, in face of the bounce-back of pest vigor, it is and needs to increase another material time point of formulation rate.In Field information
When, the medication of reference is：Citrus fruit fly sprays the desinsection containing 1.0-1.5mg/ml flue-cured tobacco extracts during 1 week -3 week of emergence
Agent；Emergence sprays the insecticide containing 0.5-1mg/ml flue-cured tobacco extracts during 3 weeks -4 weeks；Sprinkling contains during 4 weeks -5 weeks of emergence
The insecticide of 0.8-1.2mg/mL flue-cured tobacco extracts, can farthest kill citrus fruit fly and reduce cost.In addition, for
Burley tobaccos and Sun cured tobacco, can be according to the activity data of table 1, and formulation rate takes the circumstances into consideration to increase.
Claims (6)
1. tobacco extract is used for the purposes of citrus fruit fly insecticide.
2. purposes as claimed in claim 1, it is characterised in that：The tobacco is flue-cured tobacco, burley tobaccos or Sun cured tobacco.
3. purposes as claimed in claim 1, it is characterised in that the tobacco extract is prepared as follows：By cigarette
Grass is first extracted with water, is then extracted by extracting solution with adjusting PH with base to 10-14 and with non-polar organic solvent, finally that extract is dense
Contract, be drying to obtain.
A kind of 4. citrus fruit fly insecticide, it is characterised in that：Tobacco extract containing 0.8-1.4mg/mL.
A kind of 5. citrus fruit fly disinsection method, it is characterised in that：Spray the citrus fruit fly insecticide described in claim 4.
6. citrus fruit fly disinsection method as claimed in claim 5, it is characterised in that：Sprayed when citrus fruit fly sprouts wings 1 week -3 week
Spill the insecticide containing 1.2-1.4mg/mL tobacco extracts；Sprinkling is carried containing 0.8-1.0mg/mL tobaccos during 3 weeks -4 weeks of emergence
Take the insecticide of thing；Emergence sprays the insecticide containing 1.0-1.2mg/mL tobacco extracts during 4 weeks -5 weeks, can be at utmost
Kill citrus fruit fly and reduce cost in ground.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711249501.3A CN107969441B (en) | 2017-12-01 | 2017-12-01 | Tobacco extract is used for the purposes of citrus fruit fly insecticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711249501.3A CN107969441B (en) | 2017-12-01 | 2017-12-01 | Tobacco extract is used for the purposes of citrus fruit fly insecticide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107969441A true CN107969441A (en) | 2018-05-01 |
| CN107969441B CN107969441B (en) | 2019-11-29 |
Family
ID=62008699
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711249501.3A Active CN107969441B (en) | 2017-12-01 | 2017-12-01 | Tobacco extract is used for the purposes of citrus fruit fly insecticide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107969441B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521740A (en) * | 2019-08-30 | 2019-12-03 | 贵州省果树科学研究所 | Trypetid prevention and treatment liquid and its preparation method and application in a kind of flue orchard |
| CN116114718A (en) * | 2023-01-09 | 2023-05-16 | 泰安市泰山森林病虫害防治检疫站 | Pesticide beneficial to plant growth and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304337A (en) * | 2014-09-05 | 2015-01-28 | 沧州科惠物理防虫研究所 | Plant source biotype fruit fly bisexual trapping and killing agent |
| CN105379771A (en) * | 2015-12-16 | 2016-03-09 | 罗永城 | Plant-derived pesticide and its preparation method and use |
| CN105532755A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Novel plant source insecticide for fruit trees, preparation method and application thereof |
| CN106490049A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of composite pesticide for growing vegetables and preparation method thereof |
-
2017
- 2017-12-01 CN CN201711249501.3A patent/CN107969441B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104304337A (en) * | 2014-09-05 | 2015-01-28 | 沧州科惠物理防虫研究所 | Plant source biotype fruit fly bisexual trapping and killing agent |
| CN105379771A (en) * | 2015-12-16 | 2016-03-09 | 罗永城 | Plant-derived pesticide and its preparation method and use |
| CN105532755A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Novel plant source insecticide for fruit trees, preparation method and application thereof |
| CN106490049A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of composite pesticide for growing vegetables and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521740A (en) * | 2019-08-30 | 2019-12-03 | 贵州省果树科学研究所 | Trypetid prevention and treatment liquid and its preparation method and application in a kind of flue orchard |
| CN116114718A (en) * | 2023-01-09 | 2023-05-16 | 泰安市泰山森林病虫害防治检疫站 | Pesticide beneficial to plant growth and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107969441B (en) | 2019-11-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Piesik et al. | Fusarium infection in maize: volatile induction of infected and neighboring uninfected plants has the potential to attract a pest cereal leaf beetle, Oulema melanopus | |
| CN101731287A (en) | Purely natural plant insecticide | |
| CN108041083B (en) | Plant source pesticide synergist and application thereof | |
| CN107969441B (en) | Tobacco extract is used for the purposes of citrus fruit fly insecticide | |
| CN104304323B (en) | The method utilizing entomopathogenic nematode and matrine combination preventing and treating black cutworm | |
| CN104920487A (en) | Traditional Chinese medicine extract, preparation method thereof and method of utilizing same to prevent and control wheat aphid | |
| Zhang et al. | Insecticidal Activity of the Soil in the Rhizosphere of Viburnum odoratissimum against Solenopsis invicta (Hymenoptera: Formicidae) | |
| CN106508978A (en) | Smoke agent for control of greenhouse botrytis cinerea and preparation method thereof | |
| CN110973168A (en) | Agricultural spraying agent | |
| CN109042753A (en) | A kind of long-acting slow-release plant source compound Herbicidal combinations and preparation method thereof | |
| Bhandari et al. | Bioefficacy of insecticides and botanicals against cabbage butterfly, Pieris brassicae | |
| CN108684740A (en) | Environment protection type insecticidal spirit | |
| Wanner et al. | Foliar and systemic applications of neem seed extract for control of spruce budworm, Choristoneura fumiferana (Clem.)(Lepidoptera: Tortricidae), infesting black and white spruce seed orchards | |
| CN103918705B (en) | Pesticidal combination containing spiral shell worm ethyl ester and gamma cyhalothrin and application thereof | |
| Meisner et al. | Effect of an aqueous extract of neem seed kernel on larvae of the European corn borer, Ostrinia nubilalis | |
| Zhang et al. | Volatiles from essential oils of three Lamiaceae plants repel the winged cotton aphid, disturb its feeding behavior and reduce its fecundity | |
| CN106577804B (en) | A kind of shielding protection type insecticide of repellent natural enemy insect and honeybee | |
| CN108522558A (en) | A kind of crops insecticide and its preparation method and application | |
| CN112544636B (en) | Application of Daniu medicine in killing mite and pest, its biological control medicine and preparation method | |
| CN112825867B (en) | Application of Plectranthus Amboinicus in killing mite and pest, and biological control agent and preparation method thereof | |
| Seal | Management of melon thrips, Thrips palmi Karny (Thysanoptera: Thripidae) using various chemicals | |
| Abiodun et al. | Bioefficacy of Petiveria alliacea and Annona squamosa against Podagrica species of Roselle (Hibiscus sabdariffa Linn) | |
| CN112868681B (en) | Application of tupelo pterodonis in aspects of mite killing and insect killing, biological control medicament and preparation method thereof | |
| Gahukar | Bioefficacy of indigenous plant products against pests and diseases of Indian forest trees: A review | |
| Abo-Elwfa et al. | Potential molluscicidal activity of the aqueous extracts of some plants and their powders against terrestrial snail Monacha obstructa (L. Pfeiffer, 1842) under laboratory and field conditions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |