CN107802027A - A kind of method that pure tobacco oil is prepared using biology enzyme - Google Patents
A kind of method that pure tobacco oil is prepared using biology enzyme Download PDFInfo
- Publication number
- CN107802027A CN107802027A CN201710906352.7A CN201710906352A CN107802027A CN 107802027 A CN107802027 A CN 107802027A CN 201710906352 A CN201710906352 A CN 201710906352A CN 107802027 A CN107802027 A CN 107802027A
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- China
- Prior art keywords
- tobacco
- oil
- petroleum ether
- enzymolysis
- extract
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Classifications
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/26—Use of organic solvents for extraction
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Manufacture Of Tobacco Products (AREA)
Abstract
A kind of method that pure tobacco oil is prepared using biology enzyme, after handling tobacco material using complex enzyme formulation, through alcoholic extraction, the method for petroleum ether back dissolving prepares absolute oil, and wherein complex enzyme formulation vigor proportioning is：The 350U/ml of pectase 250, the 1000U/ml of cellulase 800, the 600U/ml of hemicellulase 500, the 1000U/ml of alpha amylase 800, the 1000U/ml of beta amylase 800, the 100U/ml of lipase 50.Gained absolute oil product fragrance is sent out, is plentiful thoroughly, and natural tobacco perfume (or spice) is purer, more adapts to current tobacco demand.
Description
Technical field
The present invention relates to a kind of tobacco aromaticss preparation method, more particularly to one kind using a variety of cellulase treatment offals,
The method that tobacco leaf prepares pure tobacco oil.
Technical background
Pure tobacco oil is raw material irreplaceable in perfuming cigarette, non-burning tobacco and part cigarette auxiliary materials, and it forms multiple
Miscellaneous, fragrance is plentiful, pure, and pleasant impression is comfortable.Pure tobacco oil prepared by different process has respective feature and purposes, but common process
This fragrant difference of the absolute oil fragrance and tobacco of extraction is big, and volatilization or half volatile ingredient are relatively low, and aromatic style is relatively single, it is impossible to complete
Current tobacco Related product exploitation demand is adapted to entirely.
The content of the invention
Present invention aims at providing one kind to utilize biology enzyme, the method for improving pure tobacco oil extraction process, make full use of
Tobacco material, improve tobacco extract small molecular aroma component content, make fragrance it is more plentiful, closer into this perfume of cured tobacco,
Improve tobacco extract availability.
The present invention is achieved through the following technical solutions：
A kind of method that pure tobacco oil is prepared using biology enzyme, using following steps：
1) offal or tobacco leaf are crushed to 20-80 mesh by sterilizing, are added suitable quantity of water, are stirred and evenly mixed, at 80 DEG C of -100 DEG C of sterilizings
Reason；40 DEG C of -50 DEG C of insulations；
2) enzymolysis adds complex enzyme formulation enzymolysis 1-3 hours, and regulation PH4-7 is sufficiently stirred；85 degree of alcohol circumfluence distillations
Tobacco powder after enzymolysis；Extract petroleum ether extraction, it is concentrated to give absolute oil product；Wherein, enzyme activity proportioning is：
Pectase 250-350U/ml；
Cellulase 800-1000U/ml；
Hemicellulase 500-600U/ml；
Alpha-amylase 800-1000U/ml；
Beta amylase 800-1000U/ml；
Lipase 50-100U/ml；
3), enzyme deactivation and extraction：Tobacco powder after enzymolysis is transferred to extractor, add 10 times of weight than 95% edible alcohol,
It is 85 ° to adjust alcoholic strength, circumfluence distillation 1.5 hours, after cooling, filtering, concentration filtrate, obtains tobacco extract；
4), back dissolving extracts：By tobacco extract obtained above with 5 times of weight than petroleum ether fully dissolve, clarify, mistake
Filter；After residue desolventizing insoluble in petroleum ether, a tobacco extract byproduct is obtained；Inspissated oil ether dissolves part, precipitation
Required pure tobacco oil is obtained after agent.
By enzyme-added processing, obtained pure tobacco oil and tobacco obtained by same materials traditional extraction technique are net for offal or tobacco leaf
Oil phase ratio, fragrance are sent out, are plentiful thoroughly, and natural tobacco perfume (or spice) is purer, there is more preferable availability.In perfuming cigarette, dripping pill core, electronics
Application on cigarette has certain advantage compared with absolute oil prepared by traditional handicraft.It is shown in Table 1.
Table 1 applies sense organ evaluating meter to improve absolute oil with compareing absolute oil
Table 1
Note：Different tests target organoleptic smokes panel test comprehensive grading because evaluation criterion difference does not have comparativity.
Embodiment
Embodiment one
1st, sterilize：1kg offal or tobacco leaf is weighed, is crushed to 20-80 mesh, adds 500g deionized waters, 100 revs/min are stirred
Mixing is mixed, 85 DEG C of constant temperature is heated rapidly to and sterilizes for 10 minutes, system is quickly cooled to 45 DEG C of perseverances by sterilization time after with cold water
Temperature.
2nd, digest：Tobacco powder gross weight 0.1-0.2%% (W/W) complex enzyme formulation is added, PH is adjusted with hydrochloric acid and sodium hydroxide
For 5.5, it is sufficiently stirred, enzymolysis time is 1 hour, and complex enzyme formulation enzyme activity proportioning is as follows：
Pectase 250-350U/ml
Cellulase 800-1000U/ml
Hemicellulase 500-600U/ml
Alpha-amylase 800-1000U/ml
Beta amylase 800-1000U/ml
Lipase 50-100U/ml
3rd, enzyme deactivation and extraction：Tobacco powder after enzymolysis is transferred to extractor, add 10 times of weight than 95% edible alcohol, regulation
Alcoholic strength, circumfluence distillation 1.5 hours, cooling, filtering, after concentrating filtrate, obtain tobacco extract.
4th, back dissolving extracts：By tobacco extract obtained above with 5 times of weight than petroleum ether fully dissolve, clarify, filter.
After residue desolventizing insoluble in petroleum ether, a tobacco extract byproduct is obtained；Inspissated oil ether dissolves part, after desolventizing
Obtain required pure tobacco oil.
Embodiment two
1st, sterilize：1kg offal or tobacco leaf is weighed, is crushed to 20-80 mesh, adds 500g deionized waters, 100 revs/min are stirred
Mixing is mixed, 85 DEG C of constant temperature is heated rapidly to and sterilizes for 10 minutes, system is quickly cooled to 45 DEG C of perseverances by sterilization time after with cold water
Temperature.
2nd, digest：Tobacco powder gross weight 0.1-0.2%% (W/W) complex enzyme formulation is added, PH is adjusted with hydrochloric acid and sodium hydroxide
For 5.5, it is sufficiently stirred, enzymolysis time is 2 hours, and complex enzyme formulation enzyme activity proportioning is as follows：
Pectase 250-350U/ml
Cellulase 800-1000U/ml
Hemicellulase 500-600U/ml
Alpha-amylase 800-1000U/ml
Beta amylase 800-1000U/ml
Lipase 50-100U/ml
3rd, enzyme deactivation and extraction：Tobacco powder after enzymolysis is transferred to extractor, add 10 times of weight than 95% edible alcohol, regulation
Alcoholic strength, circumfluence distillation 1.5 hours, cooling, filtering, after concentrating filtrate, obtain tobacco extract.
4th, back dissolving extracts：By tobacco extract obtained above with 5 times of weight than petroleum ether fully dissolve, clarify, filter.
After residue desolventizing insoluble in petroleum ether, a tobacco extract byproduct is obtained；Inspissated oil ether dissolves part, after desolventizing
Obtain required pure tobacco oil.
Embodiment three
1st, sterilize：1kg offal or tobacco leaf is weighed, is crushed to 20-80 mesh, adds 500g deionized waters, 100 revs/min are stirred
Mixing is mixed, 85 DEG C of constant temperature is heated rapidly to and sterilizes for 10 minutes, system is quickly cooled to 45 DEG C of perseverances by sterilization time after with cold water
Temperature.
2nd, digest：Tobacco powder gross weight 0.1-0.2%% (W/W) complex enzyme formulation is added, PH is adjusted with hydrochloric acid and sodium hydroxide
For 5.5, it is sufficiently stirred, enzymolysis time is 3 hours, and complex enzyme formulation enzyme activity proportioning is as follows：
Pectase 250-350U/ml
Cellulase 800-1000U/ml
Hemicellulase 500-600U/ml
Alpha-amylase 800-1000U/ml
Beta amylase 800-1000U/ml
Lipase 50-100U/ml
3rd, enzyme deactivation and extraction：Tobacco powder after enzymolysis is transferred to extractor, add 10 times of weight than 95% edible alcohol, regulation
Alcoholic strength, circumfluence distillation 1.5 hours, cooling, filtering, after concentrating filtrate, obtain tobacco extract.
4th, back dissolving extracts：By tobacco extract obtained above with 5 times of weight than petroleum ether fully dissolve, clarify, filter.
After residue desolventizing insoluble in petroleum ether, a tobacco extract byproduct is obtained；Inspissated oil ether dissolves part, after desolventizing
Obtain required pure tobacco oil.
Claims (1)
- A kind of 1. method that pure tobacco oil is prepared using biology enzyme, it is characterised in that：Using following steps：1), offal or tobacco leaf are crushed to 20-80 mesh by sterilizing, are added suitable quantity of water, are stirred and evenly mixed, in 80 DEG C of -100 DEG C of sterilization treatments； 40 DEG C of -50 DEG C of insulations；2), enzymolysis adds complex enzyme formulation enzymolysis 1-3 hours, and regulation PH 4-7 are sufficiently stirred；85 degree of alcohol circumfluence distillation enzymes Tobacco powder after solution；Extract petroleum ether extraction, it is concentrated to give absolute oil product；Wherein, enzyme activity proportioning is：Pectase 250-350U/ml；Cellulase 800-1000U/ml；Hemicellulase 500-600U/ml；Alpha-amylase 800-1000U/ml；Beta amylase 800-1000U/ml；Lipase 50-100U/ml；3), enzyme deactivation and extraction：Tobacco powder after enzymolysis is transferred to extractor, add 10 times of weight than 95% edible alcohol, regulation Alcoholic strength is 85 °, circumfluence distillation 1.5 hours, after cooling, filtering, concentration filtrate, obtains tobacco extract；4), back dissolving extracts：By tobacco extract obtained above with 5 times of weight than petroleum ether fully dissolve, clarify, filter；No After being dissolved in the residue desolventizing of petroleum ether, a tobacco extract byproduct is obtained；Inspissated oil ether dissolves part, is obtained after desolventizing To required pure tobacco oil.
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| CN201710906352.7A CN107802027A (en) | 2017-09-29 | 2017-09-29 | A kind of method that pure tobacco oil is prepared using biology enzyme |
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| CN201710906352.7A CN107802027A (en) | 2017-09-29 | 2017-09-29 | A kind of method that pure tobacco oil is prepared using biology enzyme |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007955A (en) * | 2018-06-21 | 2018-12-18 | 重庆中烟工业有限责任公司 | A kind of cabo extractive, preparation method and tobacco product |
| CN109280581A (en) * | 2018-09-20 | 2019-01-29 | 云南中烟工业有限责任公司 | A kind of high efficiency preparation method and purposes of pure tobacco oil |
| CN112471586A (en) * | 2020-11-20 | 2021-03-12 | 河北中烟工业有限责任公司 | Method for simultaneously preparing tobacco essential oil and tobacco juice |
| CN115088860A (en) * | 2022-07-05 | 2022-09-23 | 贵州黄果树金叶科技有限公司 | Method for preparing tobacco absolute oil by treating tobacco waste through enzymolysis, tobacco absolute oil and application |
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| CN106047491A (en) * | 2016-05-09 | 2016-10-26 | 南京泽朗医药科技有限公司 | An extracting method for oregano volatile oil |
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| JPH0568526A (en) * | 1991-09-12 | 1993-03-23 | T Hasegawa Co Ltd | Preparation of extract for tobacco flavoring |
| CN1400300A (en) * | 2001-08-01 | 2003-03-05 | 云南瑞升科技有限公司 | Method for deep-processing tobacco extract to prepare tobacco cleaned oil and enriched solanesol |
| EP1668113B1 (en) * | 2003-08-11 | 2013-06-19 | Verenium Corporation | Laccases, nucleic acids encoding them and methods for making and using them |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007955A (en) * | 2018-06-21 | 2018-12-18 | 重庆中烟工业有限责任公司 | A kind of cabo extractive, preparation method and tobacco product |
| CN109007955B (en) * | 2018-06-21 | 2021-12-31 | 重庆中烟工业有限责任公司 | Tobacco stem extract, preparation method thereof and tobacco product |
| CN109280581A (en) * | 2018-09-20 | 2019-01-29 | 云南中烟工业有限责任公司 | A kind of high efficiency preparation method and purposes of pure tobacco oil |
| CN109280581B (en) * | 2018-09-20 | 2021-07-30 | 云南中烟工业有限责任公司 | Efficient preparation method and application of tobacco absolute |
| CN112471586A (en) * | 2020-11-20 | 2021-03-12 | 河北中烟工业有限责任公司 | Method for simultaneously preparing tobacco essential oil and tobacco juice |
| CN115088860A (en) * | 2022-07-05 | 2022-09-23 | 贵州黄果树金叶科技有限公司 | Method for preparing tobacco absolute oil by treating tobacco waste through enzymolysis, tobacco absolute oil and application |
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Application publication date: 20180316 |