CN107751067B - Breeding method of all-male macrobrachium rosenbergii - Google Patents

Abstract

The invention relates to the technical field of aquaculture, in particular to a breeding method of all-male Macrobrachium rosenbergii. The breeding method of the invention puts different feeds according to the growth requirements of Macrobrachium rosenbergii in different breeding stages, promotes the maturation of the parental pseudo-female shrimp of Macrobrachium rosenbergii, effectively shortens the breeding cycle; A large number of whole male shrimp seedlings were obtained; in addition, the content of selenium in the shrimp meat was increased, and the nutritional quality of Macrobrachium rosenbergii was improved.

Description

A kind of breeding method of all male Macrobrachium rosenbergii

technical field

The invention relates to the technical field of aquaculture, in particular to a breeding method of all-male Macrobrachium rosenbergii.

Background technique

Macrobrachium rosenbergii, also known as freshwater long-armed prawn and Malaysian prawn, is native to the tropical and subtropical waters of the Indo-Pacific and is one of the largest freshwater prawns in the world. It has the advantages of wide food habits, easy domestication, strong adaptability and short production cycle. The traditional method of breeding Macrobrachium rosenbergii in China is to mix the male and female shrimp together. Generally, 2 to 3 months after the seedlings are put in, the female shrimp will begin to mature sexually and hold eggs. Nutrients are distributed to the eggs. Although male Macrobrachium rosenbergii does not hold eggs and the individual is larger than the female shrimp, when the male and female are mixed together, the male shrimp will consume nutrients during mating, which is limited in terms of growth rate and individual size. The all-male Macrobrachium rosenbergii does not have the problem of mating and nutrient loss, and maintains the advantage of being single sex, and can grow as much meat as it eats, so it can fully reflect the advantages of fast growth and large individuals after sexual maturity.

The sex of Macrobrachium rosenbergii can be completely transformed by microsurgery to control the male hormones of early male shrimp fry. The success of this transfer operation has produced a complete transformation of sex function, making male shrimp into new female shrimp ( The phenotype is female but with male genes) and has female gonads. Because male Macrobrachium rosenbergii carries two identical homologous chromosomes (ZZ), like other lobsters and shrimps studied, transgenic males can produce offspring that are 100% male. However, it is often difficult to remove the gonads at one time using microsurgery, resulting in a low number of false females obtained.

Chinese patent application (CN105647973A) provides a method for regulating male and female sex of Macrobrachium rosenbergii, which is to reduce or silence the sex of Macrobrachium rosenbergii whose in vivo sequence is SEQ ID NO: 1 before the embryonic development stage of Macrobrachium rosenbergii to the post-embryonic stage of post-larval development protein or its derived protein expression. Through RNA interference technology and gene expression regulation technology, directional sex control can induce sex reversal in shrimp, but there is a problem of low induction rate.

SUMMARY OF THE INVENTION

In order to solve the above problems, the present invention provides a breeding method of all-male Macrobrachium rosenbergii. The breeding method of the invention puts different feeds according to the growth requirements of Macrobrachium rosenbergii in different breeding stages, promotes the maturation of the parental pseudo-female shrimp of Macrobrachium rosenbergii, effectively shortens the breeding cycle; A large number of whole male shrimp seedlings were obtained; in addition, the content of selenium in the shrimp meat was increased, and the nutritional quality of Macrobrachium rosenbergii was improved.

The present invention is realized through the following scheme:

A method for breeding all-male Macrobrachium rosenbergii, comprising the following steps:

S1. The pseudo-female shrimp obtained by breeding is placed under the conditions of temperature of 26-28 ° C and fed with feed I, and raised for 14-20 days until the gonads mature, and the physique is robust, the appendages are intact, and the gonads are mature. Male shrimp mate for fertilization;

S2. After the above-mentioned step S1 male and female shrimp mating and fertilization is completed, the oviparous female shrimp is placed under the condition that the temperature is 28～30 ℃, and the feed II is put in and raised for 16 to 20 days until the color of the oviparous female shrimp abdomen embryo changes from pale yellow to When the color is light gray or gray, when the embryo develops to flea larvae, inject seawater, control the salinity at 7-9‰, and control the water temperature at 29-32℃;

S3. In the above step S2, the nauplii of M. rosenbergii are cultured for 7 to 11 days and then transferred to the larvae cultivation tank. The water salinity in the larval cultivation tank is controlled at 1.2 to 1.6%, and the water temperature is controlled at 28 ~32°C, feed III after three days;

S4. After the larvae of Macrobrachium rosenbergii have been cultivated for 20 to 22 days in the above step S3, desalinated and moved to the nursery pond. The water temperature is controlled at 26 to 28°C, and feed IV is put in.

Preferably, in the step S1, the feed I is composed of the following components and their parts by weight: 20-40 parts of soybean meal, 30-50 parts of shrimp shell powder, 1-1.5 parts of betaine, 1-5 parts of magnesium sulfate, selenium-rich 5-10 parts of yeast and 5-10 parts of additives.

Preferably, the additive is composed of baicalin, 17α-hydroxyprogesterone and serotonin in a weight ratio of 3:2:1.

Preferably, in the step S2, the feed II is composed of the following components and their parts by weight: 20-30 parts of fish meal, 15-25 parts of bone meal, 10-12 parts of soybean meal, 5-8 parts of mannose oligosaccharide and 10-8 parts of selenium-enriched wheat flour 15 servings.

Preferably, in the step S3, feed III is composed of Artemia, egg custard and papain in a weight ratio of 3:2:1.

Preferably, in the step S4, the water in the nursery pond is processed by an aquaculture ozone culture system: first, the water is filtered through a microfilter containing sponge and activated carbon, and then sand filtration is performed, and after sand filtration, 1/3 of the volume of water is directly filtered through a valve. It is discharged into the mixing tank, and the remaining 2/3 volume of water is subjected to ozone treatment through the ozone generator. The ozone-treated water is discharged into the mixing tank through the valve and mixed with the water directly discharged into the mixing tank after sand filtration, and 5-8ppm EDTA and 10～15ppm Na ₂ S ₂ O ₃ , the mixed water enters the aeration tank for aeration for 6～8 hours, the aerated water goes through a biological filter containing nitrifying bacteria for biochemical filtration, and the water after biochemical filtration is discharged into Breeding and breeding ponds; the water was not treated for the first three days after the larvae of the whole male M. rosenbergii were put in, and 1/5 to 2/5 of the water in the nursery pond was treated as above on the fourth day after the larvae of M. rosenbergii were put in.

Preferably, the feed IV in step S4 is composed of the following components and their parts by weight: 20-40 parts of shrimp meal, 10-30 parts of Artemia, 20-30 parts of selenium-enriched yeast, 1-5 parts of enzyme powder and 0.1 part of ginsenoside ~ 0.5 servings.

Preferably, the feed IV described in step S4 consists of the following components and their parts by weight: 35 parts of shrimp meal, 35 parts of Artemia, 25 parts of selenium-enriched yeast, 4.5 parts of enzyme powder and 0.5 parts of ginsenosides.

The selection and breeding of the parent pseudo-female of Macrobrachium rosenbergii in the present invention is obtained according to the method for regulating male and female sex of Macrobrachium rosenbergii in Chinese patent application (CN105647973A).

In the present invention, baicalin, 17α-hydroxyprogesterone and 5-hydroxytryptamine are added to feed I. Scutellaria baicalensis is bitter in taste and cold in nature, and has the effect of inhibiting bacteria and enhancing the immune function of the body. Scutellarin is a flavonoid extracted from the labiatae plant Scutellariae Scutellariae. It is one of the active ingredients of Scutellariae Scutellariae. , anti-inflammatory and other pharmacological effects, due to its advantages of low level, low pollution and resistance to drug resistance, it has been used in the prevention and treatment of various aquatic animals. Studies have shown that 17α-hydroxyprogesterone can significantly promote the development of ovary cells and the accumulation of yolk in shrimp or other crustaceans. Serotonin, also known as serotonin, is involved in many physiological processes such as crustacean pigment migration, reproductive activity, molting activity, skeletal muscle contraction and blood glucose concentration. Those skilled in the present invention have found that feed I of the present invention can promote the maturation of parental pseudo-female shrimp, and shorten the breeding cycle.

The feed II of the present invention mainly contains fish meal, bone meal, soybean meal and selenium-enriched wheat flour, which meets the nutritional requirements of the oviparous female shrimp; the feed II of the present invention also contains mannose oligosaccharide, and the mannose oligosaccharide can improve the crude protein, the The digestibility of crude fat and crude fiber, thereby improving the utilization rate of feed II.

The feed III of the present invention is composed of Artemia, egg custard and papain in a weight ratio of 3:2:1.

Feed IV consists of shrimp meal, Artemia, selenium-enriched yeast, enzyme powder and ginsenosides. It can not only improve the survival rate of all male shrimp seedlings, but also unexpectedly found that it can also increase the selenium content in the shrimp meat, and improve the nutritional quality of Macrobrachium rosenbergii.

In addition, the invention treats the water aquaculture ozone culture system in the fish fry pond, so as to effectively prevent the damage to the seedlings by microorganisms and viruses in the water.

Compared with the prior art, the present invention has the following technical advantages:

(1) the breeding method of the present invention can effectively promote the maturation of the parental pseudo-female shrimp, and shorten the breeding cycle;

(2) The all-male shrimp obtained by the breeding method of the present invention has a high survival rate, realizes a large number of all-male shrimp seedlings, and also increases the selenium content in the shrimp meat and improves the nutritional quality of Macrobrachium rosenbergii.

Detailed ways

The present invention will be described in further detail below in conjunction with the embodiments. It should be noted that the following descriptions are merely examples of the technical solutions claimed in the present invention, and are not intended to limit these technical solutions. The protection scope of the present invention is based on the contents described in the appended claims.

Serotonin was purchased from Shanghai Yiyan Biotechnology Co., Ltd.; selenium-enriched yeast and soybean meal were purchased from Zhengzhou Huafeng Food Technology Co., Ltd.; shrimp meal was purchased from Guangdong Xingyi Marine Biological Engineering Co., Ltd.; bone meal was purchased from Shijiazhuang Honghui Biotechnology Co., Ltd. Technology Co., Ltd.; ginsenosides were purchased from Hubei Hongyunlong Biotechnology Co., Ltd.; 17α-hydroxyprogesterone was purchased from Hubei Jusheng Technology Co., Ltd.; selenium-enriched wheat flour was purchased from Shandong Jinsui Agricultural Development Co., Ltd.

Embodiment 1 A kind of breeding method of whole male Macrobrachium rosenbergii

A method for breeding all-male Macrobrachium rosenbergii, comprising the following steps:

S1. The pseudo-female shrimp obtained by breeding is placed under the condition that the temperature is 28 ° C, and is fed with feed I, and is raised for 14 to 20 days until the gonads mature, and the male shrimp with robust physique, intact appendages, and mature gonads Carry out mating and fertilization; Described feed 1 is made up of the following components and parts by weight: 25 parts of soybean meal, 40 parts of shrimp shell powder, 1.2 parts of betaine, 3.6 parts of magnesium sulfate, 7.5 parts of selenium-enriched yeast and 6 parts of additives; Described The additive in feed I is composed of baicalin, 17α-hydroxyprogesterone and serotonin in a weight ratio of 3:2:1;

S2. After the above-mentioned step S1 male and female shrimp mating and fertilization is completed, the oviparous female shrimp is placed under the condition that the temperature is 30 ° C, and the feed II is put in for 16 to 20 days until the color of the oviparous female shrimp abdomen embryo turns from light yellow to light gray. Or gray, when the embryo develops to falid larvae, inject seawater, the salinity is controlled at 8.5‰, and the water temperature is controlled at 30 ° C; the feed II is composed of the following components and their weight parts: 25 parts of fish meal, 20 parts of bone meal, soybean meal 10 servings, 7 servings of mannose oligosaccharide and 13 servings of selenium-enriched wheat flour;

S3. In the above-mentioned step S2, the whole male Macrobrachium rosenbergii falciform nauplii are cultured for 7 to 11 days and then moved to the larvae cultivation tank. The water salinity in the larvae cultivation tank is controlled at 1.4%, and the water temperature is controlled at 30°C, Put feed III after three days; Described feed III is made up of Artemia, egg custard and papain in a weight ratio of 3:2:1;

S4. After the larvae of Macrobrachium rosenbergii are cultivated for 20-22 days in the above step S3, desalination treatment is carried out, and moved to the nursery pond, the water temperature is controlled at 28 ° C, and feed IV is put in; the feed IV is composed of the following components and parts by weight: 35 parts of shrimp powder, 35 parts of Artemia, 25 parts of selenium-enriched yeast, 4.5 parts of enzyme powder and 0.5 parts of ginsenoside.

The water in the nursery pond is treated by an aquaculture system: first, the water is filtered through a micro filter containing sponge and activated carbon, and then sand filtration. After sand filtration, 1/3 of the volume of water is directly discharged into the mixing tank through the valve, and the remaining 2 /3 volume of water is ozonated by an ozone generator, and the ozonated water is discharged into the mixing tank through a valve to mix with the water directly discharged into the mixing tank after sand filtration, and add 7ppm EDTA and 12ppm Na ₂ S ₂ O ₃ , mix The water after aeration enters the aeration tank for aeration for 6 hours, and the aerated water is then subjected to biochemical filtration through a biological filter containing nitrifying bacteria, and the biochemically filtered water is discharged into the breeding tank; The water was not treated for the first three days, and 1/5 of the water in the nursery pond was treated as above on the fourth day after the larvae of Macrobrachium rosenbergii were put in.

Embodiment 2 A kind of breeding method of whole male Macrobrachium rosenbergii

A method for breeding all-male Macrobrachium rosenbergii, comprising the following steps:

S1. The pseudo-female shrimp obtained by breeding is placed under the condition that the temperature is 26 ° C and fed with feed I, and raised for 14 to 20 days until the gonads mature, and the male shrimp with robust physique, intact appendages and mature gonads Carry out mating and fertilization; Described feed 1 is made up of the following components and parts by weight: 20 parts of soybean meal, 30 parts of shrimp shell powder, 1 part of betaine, 1 part of magnesium sulfate, 5 parts of selenium-enriched yeast and 5 parts of additives; Described The additive in feed I is composed of baicalin, 17α-hydroxyprogesterone and serotonin in a weight ratio of 3:2:1;

S2. After the above-mentioned step S1 male and female shrimp mating and fertilization is completed, the oviparous female shrimp is placed under the condition that the temperature is 30 ° C, and the feed II is put in for 16 to 20 days until the color of the oviparous female shrimp abdomen embryo turns from light yellow to light gray. Or gray, when the embryo develops to flea larvae, inject seawater, the salinity is controlled at 7‰, and the water temperature is controlled at 29°C; the feed II is composed of the following components and their weight parts: 20 parts of fish meal, 15 parts of bone meal, soybean meal 10 servings, 5 servings of mannose oligosaccharide and 10 servings of selenium-enriched wheat flour;

S3. In the above step S2, the whole male Macrobrachium rosenbergii nauplii are cultured for 7 to 11 days and then transferred to the larvae cultivation tank. The water salinity in the larval cultivation tank is controlled at 1.2%, and the water temperature is controlled at 28°C, Put feed III after three days; Described feed III is made up of Artemia, egg custard and papain in a weight ratio of 3:2:1;

S4. After the larvae of Macrobrachium rosenbergii have been cultivated for 20 to 22 days in the above step S3, desalinated and moved to the nursery pond. 20 parts of shrimp powder, 10 parts of Artemia, 20 parts of selenium-enriched yeast, 1 part of enzyme powder and 0.1 part of ginsenoside.

The water in the nursery pond is treated by an aquaculture system: first, the water is filtered through a micro filter containing sponge and activated carbon, and then sand filtration. After sand filtration, 1/3 of the volume of water is directly discharged into the mixing tank through the valve, and the remaining 2 /3 volume of water is ozonated by an ozone generator, and the ozonated water is discharged into the mixing tank through a valve to mix with the water directly discharged into the mixing tank after sand filtration, and add 5ppm EDTA and 10ppm Na ₂ S ₂ O ₃ , mix The water after the aeration enters the aeration tank for aeration for 6-8 hours, and the aerated water is then subjected to biochemical filtration through a biological filter containing nitrifying bacteria, and the biochemically filtered water is discharged into the breeding tank; all male Macrobrachium rosenbergii larvae are put in The water was not treated for the first three days after that, and 2/5 of the water in the nursery pond was treated as above on the fourth day after the larvae of Macrobrachium rosenbergii were put in.

Embodiment 3 A kind of breeding method of whole male Macrobrachium rosenbergii

A method for breeding all-male Macrobrachium rosenbergii, comprising the following steps:

S1. The pseudo-female shrimp obtained by breeding is placed under the condition that the temperature is 28 ° C, and feed I is put into it, and it is raised for 14 to 20 days until the gonads mature, and the male shrimp with robust physique, intact appendages, and mature gonads are carried out. Mating and fertilization; Described feed 1 is made up of the following components and parts by weight: 40 parts of soybean meal, 50 parts of shrimp shell powder, 1.5 parts of betaine, 5 parts of magnesium sulfate, 10 parts of selenium-enriched yeast and 10 parts of additives; Described feed In I, the additive is composed of baicalin, 17α-hydroxyprogesterone and 5-hydroxytryptamine in a weight ratio of 3:2:1;

S2. After the above-mentioned step S1 male and female shrimp mating and fertilization is completed, the oviparous female shrimp is placed under the condition that the temperature is 28 ° C, and the feed II is put in for 16 to 20 days until the color of the oviparous female shrimp abdomen embryo turns from light yellow to light gray. Or gray, when the embryo develops to flea-like larvae, inject seawater, the salinity is controlled at 9‰, and the water temperature is controlled at 32 ° C; the feed II is composed of the following components and their weight parts: 30 parts of fish meal, 25 parts of bone meal, soybean meal 12 servings, 8 servings of mannose oligosaccharide and 15 servings of selenium-enriched wheat flour;

S3. In the above step S2, the whole male M. rosenbergii falciform nauplii are cultured for 7 to 11 days and then transferred to the larvae cultivation tank. The water salinity in the larval cultivation tank is controlled at 1.6%, and the water temperature is controlled at 32°C, Put feed III after three days; Described feed III is made up of Artemia, egg custard and papain in a weight ratio of 3:2:1;

S4. After the larvae of Macrobrachium rosenbergii are cultivated for 20 to 22 days in the above step S3, desalination treatment is carried out, and the water temperature is controlled at 28 ° C, and feed IV is put in; the feed IV is composed of the following components and parts by weight: 40 servings of shrimp powder, 30 servings of Artemia, 30 servings of selenium-enriched yeast, 5 servings of enzyme powder and 0.5 servings of ginsenosides.

The water in the nursery pond is treated by an aquaculture system: first, the water is filtered through a micro filter containing sponge and activated carbon, and then sand filtration. After sand filtration, 1/3 of the volume of water is directly discharged into the mixing tank through the valve, and the remaining 2 /3 volume of water is ozonated by an ozone generator, and the ozonated water is discharged into the mixing tank through a valve to mix with the water directly discharged into the mixing tank after sand filtration, and add 8ppm EDTA and 15ppm Na ₂ S ₂ O ₃ , mix The water after aeration enters the aeration tank for aeration for 8 hours, and the aerated water is then subjected to biochemical filtration through a biological filter containing nitrifying bacteria, and the biochemically filtered water is discharged into the breeding tank; The water was not treated for the first three days, and 1/5 of the water in the nursery pond was treated as above on the fourth day after the larvae of Macrobrachium rosenbergii were put in.

Comparative Example 1 Breeding method of all-male Macrobrachium rosenbergii

The difference with Example 1 is that the feed I put in in step S1 does not contain additives, and other steps are similar to those in Example 1.

Comparative Example 2 A Breeding Method of Whole-male Macrobrachium rosenbergii

The difference from Example 1 is that β-glucan is used to replace mannose oligosaccharide in the feed II put in step S2, and other steps are similar to those of Example 1.

Comparative Example 3 A Breeding Method of Whole-male Macrobrachium rosenbergii

The difference from Example 1 is that ginsenosides are replaced with antimicrobial peptides in the feed IV put in in the step S4, and other steps are similar to those in Example 1.

Test Example 1 Determination of the maturity rate of the parent pseudo-female of Macrobrachium rosenbergii

The parental pseudo-female shrimp of Macrobrachium rosenbergii with the same ovary development obtained by breeding were cultivated for 14 days according to the breeding methods described in Examples 1 to 3 and Comparative Example 1. That is, the proportion of broodstock whose ovaries are in the IV stage. The results are shown in Table 1.

Table 1 Maturity rate of pseudo-female shrimp in different groups

group Conversion rate(%) Example 1 98.5% Example 2 96.0% Example 3 95.3% Comparative Example 1 73.0%

It can be seen from Table 1 that in Examples 1-3, the ovary maturation rate of the parent pseudo female shrimp of Macrobrachium rosenbergii was significantly higher than that of the comparative example. Among them, compared with Comparative Example 1, the maturation rate of Example 1 increased by 34.9%, indicating that the combined use of baicalin, 17α-hydroxyprogesterone and serotonin can effectively promote the maturation of the ovary of the parental pseudo female shrimp, thereby effectively shortening the reproduction time. cycle.

Test Example 2 Survival rate of all male shrimp seedlings

All male shrimp seedlings obtained by the breeding methods of Examples 1 to 3 and Comparative Example 2 were cultivated, and the survival rate was determined according to the following formula. The results are shown in Table 2.

Survival rate = (total number of juveniles - number of dead juveniles)/total number of juveniles × 100%

Table 2 Survival rate of all male Macrobrachium rosenbergii seedlings

group Survival rate (%) Example 1 93.0% Example 2 91.2% Example 3 92.6% Comparative Example 2 83.4% Comparative Example 3 88.4%

It can be seen from Table 2 that the survival rates of all-male M. rosenbergii seedlings obtained by the breeding methods of Examples 1 to 3 of the present invention are all greater than 90%. Compared with Comparative Example 2, the survival rate of Example 1 was increased by 11.5%, indicating that the addition of mannose oligosaccharide was more conducive to the utilization of feed II by all-male M. rosenbergii seedlings, thereby improving the survival rate of seedlings.

Test Example 3 Determination of Selenium Content in Whole Male Shrimp

After culturing the obtained all-male shrimp seedlings for 90 days according to the breeding methods of Examples 1 to 3 and Comparative Example 3, the selenium content of each group of shrimp was detected. The specific data are shown in Table 3.

Table 3 Determination of selenium content in whole male shrimp

group Total selenium content (μg/kg) Example 2 907.8 Example 3 908.2 Comparative Example 3 762.9

It can be seen from Table 3 that the content of selenium in the whole male shrimp meat obtained by the breeding methods of Examples 1 to 3 of the present invention is relatively high. Compared with Comparative Example 3, the total selenium content in the male shrimp meat of Example 1 is increased by 19.1%, It indicated that the combined use of ginsenosides and selenium-enriched yeast could significantly increase the content of selenium in whole male shrimp meat.

The above descriptions are only some embodiments of the present invention, but the embodiments of the present invention are not limited by the above-mentioned embodiments. For those of ordinary skill in the art, any other changes, modifications, substitutions and simplifications made without departing from the spirit and principle of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention. within.

Claims (2)

1. A breeding method of all-male macrobrachium rosenbergii is characterized by comprising the following steps:

s1, breeding the bred giant freshwater prawn parents pseudo-female prawns at the temperature of 26-28 ℃, feeding the giant freshwater prawn parents with a feed I, feeding the giant freshwater prawn parents for 14-20 days until the gonads of the giant freshwater prawn parents are mature, and mating and fertilizing the giant freshwater prawns with strong physique, complete appendages and mature gonads;

s2, after the female and male shrimps are mated and fertilized in the step S1, putting the egg-carrying female shrimps at the temperature of 28-30 ℃, feeding feed II for 16-20 days until the color of the abdominal embryos of the egg-carrying female shrimps is changed from light yellow to light gray or gray, and the embryos grow to daphnia-shaped larvae, injecting seawater, controlling the salinity to be 7-9 per thousand and controlling the water temperature to be 29-32 ℃;

s3, after culturing the full-male macrobrachium rosenbergii daphnia-shaped nauplii obtained in the step S2 for 7-11 days, transferring the cultured full-male macrobrachium rosenbergii into a larva culturing tank, controlling the water salinity in the larva culturing tank to be 1.2-1.6%, controlling the water temperature to be 28-32 ℃, and feeding feed III after three days;

s4, after the all-male macrobrachium rosenbergii larvae are cultured for 20-22 days in the step S3, desalting, transferring the macrobrachium rosenbergii larvae into a seedling raising pool, controlling the water temperature to be 26-28 ℃, and feeding a feed IV;

the feed I in the step S1 comprises the following components in parts by weight: 20-40 parts of soybean meal, 30-50 parts of shrimp shell powder, 1-1.5 parts of betaine, 1-5 parts of magnesium sulfate, 5-10 parts of selenium-enriched yeast and 5-10 parts of an additive;

the additive consists of baicalin, 17 alpha-hydroxyprogesterone and 5-hydroxytryptamine according to the weight ratio of 3:2: 1;

the feed II in the step S2 comprises the following components in parts by weight: 20-30 parts of fish meal, 15-25 parts of bone meal, 10-12 parts of soybean meal, 5-8 parts of mannan oligosaccharide and 10-15 parts of selenium-enriched wheat flour;

the feed III in the step S3 consists of the artemia, the egg custard and the papain in a weight ratio of 3:2: 1;

and step S4, treating the water in the nursery pond through an aquatic product ozone culture system: firstly, filtering water through a microfilter containing sponge and activated carbon, then carrying out sand filtration, directly discharging 1/3 volume of water into a mixing tank through a valve after the sand filtration, carrying out ozone treatment on the residual 2/3 volume of water through an ozone generator, discharging the water subjected to the ozone treatment into the mixing tank through the valve, mixing the water directly discharged into the mixing tank after the sand filtration, adding 5-8 ppm of EDTA and 10-15 ppm of Na₂S₂O₃Aerating the mixed water in an aeration tank for 6-8h, biochemically filtering the aerated water through a biological filter containing nitrobacteria, and discharging the biochemically filtered water into a breeding tank; the water is not treated in the first three days after the full-male macrobrachium rosenbergii larvae are put in, and the water in 1/5-2/5 in the nursery pond is treated every day in the fourth day after the macrobrachium rosenbergii larvae are put in;

the feed IV in the step S4 comprises the following components in parts by weight: 20-40 parts of shrimp meal, 10-30 parts of fairy shrimp, 20-30 parts of selenium-enriched yeast, 1-5 parts of ferment powder and 0.1-0.5 part of ginsenoside.

2. The breeding method of the all-male macrobrachium rosenbergii according to claim 1, wherein the feed IV in the step S4 comprises the following components in parts by weight: 35 parts of shrimp meal, 35 parts of fairy shrimp, 25 parts of selenium-enriched yeast, 4.5 parts of ferment powder and 0.5 part of ginsenoside.