CN107754960B - The micro-fluidic chip of the asymmetric division of double emulsion droplets is realized based on additional flow - Google Patents
The micro-fluidic chip of the asymmetric division of double emulsion droplets is realized based on additional flow Download PDFInfo
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0883—Serpentine channels
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Abstract
The present invention relates to a kind of micro-fluidic chips that the asymmetric division of double emulsion droplets is realized based on additional flow.Feature of present invention is to be bonded to fix with PDMS bottom plate using main body solid structure, and the alignment of double emulsion droplets kernels and shell is maintained using the radially fixed channel of double emulsion droplets, is ejected drop using necking is focused, and so that it is contacted double emulsion droplets divisions fork and divides.Unilateral branch flow resistance is adjusted using branch additional flow, realizes the asymmetric division of double emulsion droplets.The present invention can be in the case where not changing channel geometry, the asymmetric branch pressure generated using unilateral branch additional flow, so that double emulsion droplets are by occurring asymmetric division when Y type microchannel.By above functions, it can provide size controllable double emulsion droplets templates for capsule-type slow releasing pharmaceutical, improve the application range of slow release drug.
Description
Technical field
The present invention relates to a kind of micro-fluidic chips that the asymmetric division of double emulsion droplets is realized based on additional flow.The invention belongs to
In the drop microfluidic art based on micro-fluidic chip.
Background technique
With the continuous development of drug development level, many newtype drugs are developed to cope with different types of disease
Disease, but traditional methods of administration rises rapidly the blood concentration of patient's body in a short time simultaneously quickly
Decline.Over the course for the treatment of, blood concentration be either more than patient maximum tolerated dose again below effective dose, all can not
Obtain the effective treatment effect, can also generate undesirable side effect to patient sometimes.Although frequent low dose of administration can be with one
Determine to solve above situation in degree, but patient is often made to be difficult to receive, has implemented many difficulties.Therefore, it can slowly release
The slow release drug for putting drug ingedient comes into being.
Drop microflow control technique is that a kind of research micro-meter scale microlayer model to grow up in recent years is generated, manipulates and applied
New technology.The technology has the characteristics that stable, easy to operate and applied widely, is widely used to medicament transport, disease at present
The numerous areas such as disease protection, cell research and functional material preparation are the research hotspots of domestic and international each related discipline at present.The skill
Art, which is based on glass micro capillary device, can be generated the good double emulsion droplets of monodispersity, these drops can be later use
The microcapsules that the modes such as polymerize, ultraviolet light solidifies generate provide the template of high quality.In medicament slow release field, thickness is determined
Microcapsules shell patient's body rate of dissolution, by the way that the microcapsules of a variety of shell thickness are injected patient's body, so that different IPs
The microcapsules of shell size are realized in different time points to be discharged in batches, and it is slow with given rate in patient's body to can control drug
Release, maintains effective blood concentration.
Pertinent literature points out that the size of double emulsion droplets is largely dependent upon the geometric parameter of capillary generating means,
Due to the difficulty and complexity of capillary generating means manufacture craft, so that accurately generating different nucleocapsid sizes, especially 100 is micro-
Rice double emulsion droplets templates below also relative difficulty.
Summary of the invention
Based on current background, the present invention is to utilize unilateral branch in the case where not changing channel geometry
The asymmetric branch pressure that additional flow generates, so that double emulsion droplets are by occurring asymmetric division when Y type microchannel.Pass through
Above functions can provide size controllable double emulsion droplets templates for capsule-type slow releasing pharmaceutical, improve answering for slow release drug
Use range.
The present invention provides a kind of micro-fluidic chip that the asymmetric division of double emulsion droplets is realized based on additional flow.This is micro-fluidic
Chip includes double emulsion droplets injection inlets 1, snakelike Pressure channel 2, the double creams of double emulsion droplets movement velocitys adjusting the 3, first son of entrance
Drop outlets 4,5, first pairs, double emulsion droplets division fork emulsion droplets division branch 6, unilateral branch additional flow entrance 7, first
Export snakelike Pressure channel 8, the current stabilization main channel double emulsion droplets of the 9, second son export 10, the radially fixed channel 11 of double emulsion droplets, double
Emulsion droplets division focuses 12, second pairs of emulsion droplets of necking and divides branch 13, the snakelike Pressure channel 14 of second outlet, main body solid knot
Structure 15 and PDMS bottom plate 16.
Specifically, double emulsion droplets injection inlets 1, double emulsion droplets movement velocitys adjust entrance 3, unilateral branch additional flow
Hole configurations based on the double double emulsion droplets outlets 4 of the 10, first son of emulsion droplets outlet of the 7, second son of entrance on solid structure 15.Snake
The snakelike Pressure channel 14 of shape Pressure channel 2, second outlet, the snakelike Pressure channel 8 of first outlet, the radially fixed channel of double emulsion droplets
11,13, first pairs of emulsion droplets division branches 6 of the emulsion droplets division branch of current stabilization main channel 9, second pairs are then on body solid structure 1
Groove structure.
The main body solid structure 15 and PDMS bottom plate 16 are fixed by bonding up and down, and PDMS bottom plate 16 is placed in main body solid
Under structure 15, to supportive body solid structure 15 and it is closed the groove structure on 15 surface of main body solid structure, forms fluid
Flow region.
Double emulsion droplets injection inlets 1 are used for connecting conduit, and double emulsion droplets injection inlets 1 are directly connected to current stabilization main channel
9, double emulsion droplets movement velocitys adjust entrance 3 and connect snakelike Pressure channel 2, and the snakelike access of Pressure channel 2 is in current stabilization main channel 9
Side.
The radially fixed channel 11 of double emulsion droplets has been accessed in the tail portion of the current stabilization main channel 9, radially fixed in double emulsion droplets
The tail portion in channel 11 is that double emulsion droplets divisions focus necking 12 and double emulsion droplets division fork 5.
The two sides at double emulsion droplets divisions fork 5 are second pair of emulsion droplets division 13, first pairs of emulsion droplets of branch respectively
Branch 6 is divided, the snakelike Pressure channel 14 of second outlet has been accessed in second pair of emulsion droplets division 13 tail portion of branch.First pair of emulsion droplets
The snakelike Pressure channel 8 of first outlet has been accessed in division 6 tail portion of branch, in first pair of emulsion droplets division branch 6 and first outlet
Unilateral branch additional flow entrance 7 is connected between snakelike Pressure channel 8.
First pair of emulsion droplets division 6 end of branch is by the double emulsion droplets of snakelike first son of the access of Pressure channel 8 of first outlet
Outlet 4.The snakelike Pressure channel 14 of second outlet, the second son of access are passed through in second pair of emulsion droplets division, 13 end of branch respectively
Double emulsion droplets outlets 10；It the double double emulsion droplets outlets 4 of the 10, first son of emulsion droplets outlet of second son will conduit in succession.
2. using the method for chip as described in the appended claim 1, it is characterised in that:
The double emulsion droplets prepared are inputted into double emulsion droplets injection inlets 1, as fluid flows, double emulsion droplets by conduit
Current stabilization main channel 9 is flowed into snakelike 2 intersection of Pressure channel.On the other hand, fluid adjusts entrance 3 from double emulsion droplets movement velocitys
The micro-fluidic chip that main body solid structure 15 and PDMS bottom plate 16 are fabricated to is flowed into, by snakelike Pressure channel 2, with double emulsion droplets
Enter current stabilization main channel 9 simultaneously.By adjusting uninterrupted of the fluid from double emulsion droplets movement velocitys adjusting entrance 3, control is double
The movement velocity of emulsion droplets adjusts division stability.
Then, double emulsion droplets flow into the end of current stabilization main channel 9, into the radially fixed channel 11 of double emulsion droplets, double lotions
It drips radially fixed channel 11 double emulsion droplets are radially fixed, is divided by double emulsion droplets and focus necking 12, double emulsion droplets are penetrated
Enter double emulsion droplets divisions fork 5 to divide.On the other hand, unilateral branch additional flow entrance 7 accesses additional flow, causes the
Pressure difference inside two pairs of emulsion droplets division branches, 13, first pairs of emulsion droplets division branches 6.Finally, double emulsion droplets are in double lotions
Asymmetric division occurs for drop division fork 5, by adjusting the uninterrupted of unilateral branch additional flow entrance 7, adjusts double lotions
The division ratio of drop.
After completing asymmetric division, the double emulsion droplets of son flow through second pair of emulsion droplets division 13, first pairs of lotion of branch
Drop division branch 6, respectively enters the snakelike Pressure channel 14 of second outlet, the snakelike Pressure channel 8 of first outlet.Finally, from second
Sub double 4 inputs of emulsion droplets outlet of double the 10, first sons of emulsion droplets outlet, flow into external catheter.
More specifically, which includes double emulsion droplets injection inlets 1, snakelike Pressure channel 2, the movement of double emulsion droplets
Speed adjusts the double emulsion droplets outlets 4 of the 3, first son of entrance, double emulsion droplets divide 5, first pairs, fork emulsion droplets division branch 6, single
It is the double emulsion droplets outlets 10 of collateral road additional flow entrance 7, the snakelike Pressure channel 8 of first outlet, of current stabilization main channel 9, second, double
The radially fixed channel 11 of emulsion droplets, the division of double emulsion droplets focus 12, second pairs of emulsion droplets of necking and divide branch 13, second outlet snake
Shape Pressure channel 14, main body solid structure 15, PDMS bottom plate 16.
Specifically, double emulsion droplets injection inlets 1, double emulsion droplets movement velocitys adjust entrance 3, unilateral branch additional flow
Hole configurations based on the double double emulsion droplets outlets 4 of the 10, first son of emulsion droplets outlet of the 7, second son of entrance on solid structure 15.Snake
The snakelike Pressure channel 14 of shape Pressure channel 2, second outlet, the snakelike Pressure channel 8 of first outlet, the radially fixed channel of double emulsion droplets
11,13, first pairs of emulsion droplets division branches 6 of the emulsion droplets division branch of current stabilization main channel 9, second pairs are then on body solid structure 1
Groove structure.Wherein, each structure is the flow region of fluid when micro-fluidic chip works.
The main body solid structure 15 and PDMS bottom plate 16 are fixed by bonding up and down, and PDMS bottom plate 16 is placed in main body solid
Under structure 15, to supportive body solid structure 15 and it is closed the groove structure on 15 surface of main body solid structure, forms fluid
Flow region.
The main body solid structure 15 and PDMS bottom plate 16, which are poured and be heating and curing by dimethyl silicone polymer, to be made,
With the good transparency, it can facilitate and utilize emulsion droplets fragmentation conditions double inside microscopic micro-fluidic chip.
Double emulsion droplets injection inlets 1 are used for connecting conduit, to inject double emulsion droplets of the size uniformity prepared,
Double emulsion droplets injection inlets 1 are directly connected to current stabilization main channel 9, the conveying for double emulsion droplets.Double emulsion droplets movement velocitys are adjusted
Entrance 3 connects snakelike Pressure channel 2, and the snakelike access of Pressure channel 2 is in the side of current stabilization main channel 9, for adjusting double emulsion droplets
Spacing distance and travelling speed.
The radially fixed channel 11 of double emulsion droplets has been accessed in the tail portion of the current stabilization main channel 9, for maintaining in double emulsion droplets
The alignment of core and shell is that double emulsion droplets divisions focus neckings 12 and double in the tail portion in the radially fixed channel 11 of double emulsion droplets
Emulsion droplets divide fork 5, the practical division for double emulsion droplets.
The two sides at double emulsion droplets divisions fork 5 are second pair of emulsion droplets division 13, first pairs of emulsion droplets of branch respectively
Branch 6 is divided, the snakelike Pressure channel 14 of second outlet has been accessed in second pair of emulsion droplets division 13 tail portion of branch.First pair of emulsion droplets
The snakelike Pressure channel 8 of first outlet has been accessed in division 6 tail portion of branch, in first pair of emulsion droplets division branch 6 and first outlet
It is connected to unilateral branch additional flow entrance 7 between snakelike Pressure channel 8, for changing additional flow, causes second pair of emulsion droplets
Divide the pressure difference inside 13, first pairs of emulsion droplets division branches 6 of branch.
Second outlet is passed through in second pair of emulsion droplets division branch, 13, first pairs of emulsion droplets division 6 ends of branch respectively
The double lotions of snakelike double the 10, first sons of emulsion droplets outlet of second son of the access of Pressure channel 8 of snakelike Pressure channel 14, first outlet ooze
Mouth 4.The double double emulsion droplets outlets 4 of the 10, first son of emulsion droplets outlet of second son will conduit in succession, the son pair obtained for export division
Emulsion droplets.
The course of work of the invention is as follows, and the double emulsion droplets prepared are inputted double emulsion droplets injection inlets 1 by conduit,
As fluid flows, double emulsion droplets flow into current stabilization main channel 9 in snakelike 2 intersection of Pressure channel.On the other hand, fluid is from double creams
Liquid drop movement speed adjusts entrance 3 and flows into the micro-fluidic chip that main body solid structure 15 and PDMS bottom plate 16 are fabricated to, by snake
Shape Pressure channel 2 enters current stabilization main channel 9 with double emulsion droplets simultaneously.It is adjusted by adjusting fluid from double emulsion droplets movement velocitys
The uninterrupted of entrance 3 can control the movement velocity of double emulsion droplets, adjust division stability.
Then, double emulsion droplets flow into the end of current stabilization main channel 9, into the radially fixed channel 11 of double emulsion droplets, double lotions
It drips radially fixed channel 11 double emulsion droplets are radially fixed, is divided by double emulsion droplets and focus necking 12, double emulsion droplets are penetrated
Enter double emulsion droplets divisions fork 5 to divide.On the other hand, unilateral branch additional flow entrance 7 accesses additional flow, causes the
Pressure difference inside two pairs of emulsion droplets division branches, 13, first pairs of emulsion droplets division branches 6.Finally, double emulsion droplets are in double lotions
Asymmetric division occurs for drop division fork 5, can be further by adjusting the uninterrupted of unilateral branch additional flow entrance 7
Adjust the division ratio of double emulsion droplets.
After completing asymmetric division, the double emulsion droplets of son flow through second pair of emulsion droplets division 13, first pairs of lotion of branch
Drop division branch 6, respectively enters the snakelike Pressure channel 14 of second outlet, the snakelike Pressure channel 8 of first outlet.Finally, from second
Sub double 4 inputs of emulsion droplets outlet of double the 10, first sons of emulsion droplets outlet, flow into external catheter.It is small due to exterior pressure
Fluctuation can cause to divide the unstable of ratio, and the snakelike Pressure channel 14 of second outlet, the snakelike Pressure channel 8 of first outlet can be used for mentioning
High micro-fluidic chip internal pressure reduces the relative size of exterior slight pressure fluctuation, reduces its influence.
Compared with prior art, the present invention has the advantages that.
1, novel place of the invention is to be different to prepare the double creams of different sizes using the generating means of different dimensional parameters
The double emulsion droplets preparation methods of the tradition of drop, various sizes of double lotions are obtained in the case where not changing device geometric parameter
Drop.
2, novel place of the invention is the divisional mode in conjunction with previous single emulsion drop, is adjusted using branch additional flow single
Collateral road flow resistance realizes the asymmetric division of double emulsion droplets, controls the size of double emulsion droplets.
Detailed description of the invention
Fig. 1 is the three-dimensional perspective that the micro-fluidic chip of the asymmetric division of double emulsion droplets is realized the present invention is based on additional flow
Figure.
Fig. 2 is the top view that the micro-fluidic chip of the asymmetric division of double emulsion droplets is realized the present invention is based on additional flow.
Include in figure double emulsion droplets injection inlets 1, snakelike Pressure channel 2, double emulsion droplets movement velocitys adjust entrance 3,
The double emulsion droplets outlets 4 of first son, 5, first pairs, double emulsion droplets division fork emulsion droplets division branch 6, unilateral branch additional flow
The snakelike Pressure channel 8 of entrance 7, first outlet, the double emulsion droplets outlets 10 of the 9, second son of current stabilization main channel, double emulsion droplets are radially fixed
Channel 11, the division of double emulsion droplets focus necking 12, second pairs of emulsion droplets division branches 13, the snakelike Pressure channel 14 of second outlet,
Main body solid structure 15, PDMS bottom plate 16.
Specific embodiment
The working principle of invention and effect are described further and are verified with reference to the accompanying drawing.
Fig. 1 is the three-dimensional perspective that the micro-fluidic chip of the asymmetric division of double emulsion droplets is realized the present invention is based on additional flow
Figure.The micro-fluidic chip includes main body solid structure 15, double emulsion droplets injection inlets 1, double emulsion droplets movement velocitys adjusting entrance
3, the double emulsion droplets of unilateral double the 10, first sons of emulsion droplets outlet of the 7, second son of branch additional flow entrance export 4, snakelike Pressure channel
2, the snakelike Pressure channel 14 of second outlet, the snakelike Pressure channel 8 of first outlet, the radially fixed channel 11 of double emulsion droplets, double lotions
Drop division focuses necking 12, double emulsion droplets division fork 5, emulsion droplets division branch 13, first pairs of current stabilization main channel 9, second pairs
Emulsion droplets divide branch 6, PDMS bottom plate 16.
Specifically, double emulsion droplets injection inlets 1, double emulsion droplets movement velocitys adjust entrance 3, unilateral branch additional flow
Hole configurations based on the double double emulsion droplets outlets 4 of the 10, first son of emulsion droplets outlet of the 7, second son of entrance on solid structure 15.Snake
The snakelike Pressure channel 14 of shape Pressure channel 2, second outlet, the snakelike Pressure channel 8 of first outlet, the radially fixed channel of double emulsion droplets
11,13, first pairs of emulsion droplets division branches 6 of the emulsion droplets division branch of current stabilization main channel 9, second pairs are then on body solid structure 1
Groove structure.Wherein, each structure is the flow region of fluid when micro-fluidic chip works.
The main body solid structure 15 and PDMS bottom plate 16 are fixed by bonding up and down, and PDMS bottom plate 16 is placed in main body solid
Under structure 15, to supportive body solid structure 15 and it is closed the groove structure on 15 surface of main body solid structure, forms fluid
Flow region.
The main body solid structure 15 and PDMS bottom plate 16, which are poured and be heating and curing by dimethyl silicone polymer, to be made,
With the good transparency, it can facilitate and utilize emulsion droplets fragmentation conditions double inside microscopic micro-fluidic chip.
Double emulsion droplets injection inlets 1 are used for connecting conduit, to inject double emulsion droplets of the size uniformity prepared,
Double emulsion droplets injection inlets 1 are directly connected to current stabilization main channel 9, the conveying for double emulsion droplets.Double emulsion droplets movement velocitys are adjusted
Entrance 3 connects snakelike Pressure channel 2, and the snakelike access of Pressure channel 2 is in the side of current stabilization main channel 9, for adjusting double emulsion droplets
Spacing distance and travelling speed.
The radially fixed channel 11 of double emulsion droplets has been accessed in the tail portion of the current stabilization main channel 9, for maintaining in double emulsion droplets
The alignment of core and shell is that double emulsion droplets divisions focus neckings 12 and double in the tail portion in the radially fixed channel 11 of double emulsion droplets
Emulsion droplets divide fork 5, the practical division for double emulsion droplets.
The two sides at double emulsion droplets divisions fork 5 are second pair of emulsion droplets division 13, first pairs of emulsion droplets of branch respectively
Branch 6 is divided, the snakelike Pressure channel 14 of second outlet has been accessed in second pair of emulsion droplets division 13 tail portion of branch.First pair of emulsion droplets
The snakelike Pressure channel 8 of first outlet has been accessed in division 6 tail portion of branch, in first pair of emulsion droplets division branch 6 and first outlet
It is connected to unilateral branch additional flow entrance 7 between snakelike Pressure channel 8, for changing additional flow, causes second pair of emulsion droplets
Divide the pressure difference inside 13, first pairs of emulsion droplets division branches 6 of branch.
It is double that second pair of emulsion droplets division branch, 13, first pairs of emulsion droplets division 6 ends of branch are respectively connected to the second son
The double emulsion droplets outlets 4 of the 10, first son of emulsion droplets outlet.The double double emulsion droplets outlets 4 of the 10, first son of emulsion droplets outlet of second son will connect
Even conduit, for exporting the double emulsion droplets of son that division obtains.
The course of work of the invention is as follows, and the double emulsion droplets prepared are inputted double emulsion droplets injection inlets 1 by conduit,
As fluid flows, double emulsion droplets flow into current stabilization main channel 9 in snakelike 2 intersection of Pressure channel.On the other hand, fluid is from double creams
Liquid drop movement speed adjusts entrance 3 and flows into the micro-fluidic chip that main body solid structure 15 and PDMS bottom plate 16 are fabricated to, by snake
Shape Pressure channel 2 enters current stabilization main channel 9 with double emulsion droplets simultaneously.It is adjusted by adjusting fluid from double emulsion droplets movement velocitys
The uninterrupted of entrance 3 can control the movement velocity of double emulsion droplets, adjust division stability.
Then, double emulsion droplets flow into the end of current stabilization main channel 9, into the radially fixed channel 11 of double emulsion droplets, double lotions
It drips radially fixed channel 11 double emulsion droplets are radially fixed, is divided by double emulsion droplets and focus necking 12, double emulsion droplets are penetrated
Enter double emulsion droplets divisions fork 5 to divide.On the other hand, unilateral branch additional flow entrance 7 accesses additional flow, causes the
Pressure difference inside two pairs of emulsion droplets division branches, 13, first pairs of emulsion droplets division branches 6.Finally, double emulsion droplets are in double lotions
Asymmetric division occurs for drop division fork 5, can be further by adjusting the uninterrupted of unilateral branch additional flow entrance 7
Adjust the division ratio of double emulsion droplets.
After completing asymmetric division, the double emulsion droplets of son flow through second pair of emulsion droplets division 13, first pairs of lotion of branch
Drop division branch 6, respectively enters the snakelike Pressure channel 14 of second outlet, the snakelike Pressure channel 8 of first outlet.Finally, from second
Sub double 4 inputs of emulsion droplets outlet of double the 10, first sons of emulsion droplets outlet, flow into external catheter.It is small due to exterior pressure
Fluctuation can cause to divide the unstable of ratio, and the snakelike Pressure channel 14 of second outlet, the snakelike Pressure channel 8 of first outlet can be used for mentioning
High micro-fluidic chip internal pressure reduces the relative size of exterior slight pressure fluctuation, reduces its influence.
Claims (2)
1. a kind of micro-fluidic chip for realizing the asymmetric division of double emulsion droplets based on additional flow, it is characterised in that: this is micro-fluidic
Chip includes double emulsion droplets injection inlets, snakelike Pressure channel, double emulsion droplets movement velocitys adjusting entrance, the double emulsion droplets of the first son
Outlet, double emulsion droplets division fork, first pair of emulsion droplets division branch, unilateral branch additional flow entrance, first outlet are snakelike
Pressure channel, current stabilization main channel, the double emulsion droplets outlets of the second son, the radially fixed channel of double emulsion droplets, the division of double emulsion droplets focus
Necking, second pair of emulsion droplets division branch, the snakelike Pressure channel of second outlet, main body solid structure and PDMS bottom plate；
Double emulsion droplets injection inlets, double emulsion droplets movement velocitys adjust entrance, unilateral branch additional flow entrance, the double creams of the second son
Hole configurations based on the double emulsion droplets outlets of drop outlets, the first son on solid structure；Snakelike Pressure channel, second outlet snake
The snakelike Pressure channel of shape Pressure channel, first outlet, the radially fixed channel of double emulsion droplets, current stabilization main channel, second pair of emulsion droplets
Divide branch, first pair of emulsion droplets division branch then based on groove structure on solid structure；
The main body solid structure and PDMS bottom plate are fixed by bonding up and down, and PDMS bottom plate is placed under main body solid structure,
To supportive body solid structure and it is closed the groove structure on main body solid structure surface, forms the flow region of fluid；
Double emulsion droplets injection inlets are used for connecting conduit, and double emulsion droplets injection inlets are directly connected to current stabilization main channel, double creams
Liquid drop movement speed adjusts entrance and connects snakelike Pressure channel, side of the snakelike Pressure channel access in current stabilization main channel；
The radially fixed channel of double emulsion droplets, the tail in the radially fixed channel of double emulsion droplets have been accessed in the tail portion of the current stabilization main channel
Portion is that double emulsion droplets divisions focus necking and double emulsion droplets division fork；
The two sides at double emulsion droplets divisions fork are second pair of emulsion droplets division branch, first pair of emulsion droplets division branch respectively
The snakelike Pressure channel of second outlet has been accessed in road, second pair of emulsion droplets division branch tail portion；First pair of emulsion droplets divides branch tail
Portion has accessed the snakelike Pressure channel of first outlet, first pair of emulsion droplets division branch and the snakelike Pressure channel of first outlet it
Between be connected to unilateral branch additional flow entrance；
First pair of emulsion droplets division branch end is by the snakelike double emulsion droplets outlets of the first son of Pressure channel access of first outlet；Institute
State that the snakelike Pressure channel of second outlet is passed through in second pair of emulsion droplets division branch end respectively, the double lotions of the second son of access ooze
Mouthful；The double emulsion droplets outlet of second son, the double emulsion droplets outlets of the first son will conduits in succession.
2. using the method for chip as described in the appended claim 1, it is characterised in that:
The double emulsion droplets prepared are inputted into double emulsion droplets injection inlets by conduit, as fluid flows, double emulsion droplets are flowed into
Current stabilization main channel and snakelike Pressure channel intersection；On the other hand, fluid adjusts entrance from double emulsion droplets movement velocitys and flows into master
The micro-fluidic chip that body solid structure and PDMS bottom plate are fabricated to enters surely by snakelike Pressure channel with double emulsion droplets simultaneously
Flow main channel；By adjusting uninterrupted of the fluid from double emulsion droplets movement velocitys adjusting entrance, the movement of double emulsion droplets is controlled
Speed adjusts division stability；
Then, double emulsion droplets flow into the end of current stabilization main channel, and into the radially fixed channel of double emulsion droplets, double emulsion droplets are radially solid
Routing is radially fixed by double emulsion droplets, divides by double emulsion droplets and focuses necking, and double emulsion droplets inject double emulsion droplets point
Fork is split to divide；On the other hand, unilateral branch additional flow entrance accesses additional flow, causes second pair of emulsion droplets division
Pressure difference inside branch, first pair of emulsion droplets division branch；Finally, double emulsion droplets occur non-right at double emulsion droplets division fork
Claim division, by adjusting the uninterrupted of unilateral branch additional flow entrance, adjusts the division ratio of double emulsion droplets；
After completing asymmetric division, the double emulsion droplets of son flow through second pair of emulsion droplets division branch, first pair of emulsion droplets division
Branch respectively enters the snakelike Pressure channel of second outlet, the snakelike Pressure channel of first outlet；Finally, from the double emulsion droplets of the second son
Outlet, the double emulsion droplets outlet inputs of the first son, flow into external catheter.
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